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genome characterization of sugarcane yellow leaf virus from china reveals a novel recombinant genotype.sugarcane yellow leaf virus (scylv; genus polerovirus, family luteoviridae) is a recombinant virus associated with yellow leaf disease, a serious threat to sugarcane in china and worldwide. among the nine known scylv genotypes existing worldwide, col, haw, reu, ind, chn1, chn2, bra, cub and per, the last five have been reported in china. in this study, the complete genome sequences (5,880 nt) of gz-gz18 and hn-cp502 isolates from the chinese provinces of guizhou and hainan, respectively, were cl ...201424395076
molecular characterization and phylogenetic analysis of sugarcane yellow leaf virus isolates from china.sugarcane yellow leaf virus (scylv) (genus polerovirus, family luteoviridae), the causal agent of sugarcane yellow leaf disease (yld), was first detected in china in 2006. to assess the distribution of scylv in the major sugarcane-growing chinese provinces, leaf samples from 22 sugarcane clones (saccharum spp. hybrid) showing yld symptoms were collected and analyzed for infection by the virus using reverse transcription pcr (rt-pcr), quantitative rt-pcr, and immunological assays. a complete geno ...201222752564
putative recombination signature and significance of insertion/deletion events in the rna-dependent rna polymerase coding region of sugarcane yellow leaf virus.the 5898 nucleotide single-strand rna genome of sugarcane yellow leaf virus (scylv) contains one long open reading frame, which is translated into a 120.6 kda polyprotein. the sequences of scylv isolates from the two scylv-susceptible cultivars from hawaii had a deletion of 48-51 nt in orf1. scylv from 12 sugarcane hybrid cultivars from different origins were tested by rt-pcr using a specific set of primers, to investigate the genome segment for this deletion. only three cultivars were found not ...201222542996
production and partial characterization of cellulases and xylanases from trichoderma atroviride 676 using lignocellulosic residual biomass.trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. best results were obtained when 3.0 % (w/v) untreated sugarcane bagasse was used (61.3 u ml(-1) for xylanase, 1.9 u ml(-1) for endoglucanase, 0.25 u ml(-1) for fpase, and 0.17 u ml(-1) for β-glucosidase) after 3-4 days fermentation. the maximal enzymatic activity for endoglucanase, fpase, and xylanase were observed at 50-60 °c and ph 4.0-5 ...201323306885
the relation between xyr1 overexpression in trichoderma harzianum and sugarcane bagasse saccharification performance.this work investigates the influence of the positive regulator xyr1 of trichoderma harzianum on the production of cellulolytic enzymes, using sugarcane bagasse as carbon source. constitutive expression of xyr1 was achieved under the control of the strong trichoderma reesei pki1 promoter. five clones with xyr1 overexpression achieved higher xyr1 expression and greater enzymatic productivity when cultivated under submerged fermentation, hence validating the genetic construction for t. harzianum. c ...201728192217
addition of surfactants and non-hydrolytic proteins and their influence on enzymatic hydrolysis of pretreated sugarcane bagasse.poly(ethylene glycol) (peg 4000) and bovine serum albumin (bsa) were investigated with the purpose of evaluating their influence on enzymatic hydrolysis of sugarcane bagasse. effects of these supplements were assayed for different enzymatic cocktails (trichoderma harzianum and penicillium funiculosum) that acted on lignocellulosic material submitted to different pretreatment methods with varying solid (25 and 100 g/l) and protein (7.5 and 20 mg/g cellulose) loadings. the highest levels of glucos ...201727631122
enhanced cellulase production by trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates.the use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. this work evaluates the production of cellulase enzymes using glycerol for high cell density growth of trichoderma harzianum followed by induction with a cellulosic material. firstly, the influence of the carbon source used in the pre-culture step was investigated in terms o ...201626883662
purification and characterization of endo β-1,4-d-glucanase from trichoderma harzianum strain hzn11 and its application in production of bioethanol from sweet sorghum bagasse.an acidophilic-solvent-thermostable endo β-1,4-d-glucanase produced from a potential trichoderma harzianum strain hzn11 was purified to homogeneity by deae-sepharose and sephadex g-100 chromatography with 33.12 fold purification with specific activity of 66.25 u/mg and molecular mass of ~55 kda. the optimum temperature and ph were 60 °c and 5.5 retaining 76 and 85 % of activity after 3 h, respectively. it showed stability between ph 4.5-6.0 and temperature between 50-70 °c indicating thermostabi ...201628330171
mathematical modeling of enzyme production using trichoderma harzianum p49p11 and sugarcane bagasse as carbon source.a mathematical model to describe the kinetics of enzyme production by the filamentous fungus trichoderma harzianum p49p11 was developed using a low cost substrate as main carbon source (pretreated sugarcane bagasse). the model describes the cell growth, variation of substrate concentration and production of three kinds of enzymes (cellulases, beta-glucosidase and xylanase) in different sugarcane bagasse concentrations (5; 10; 20; 30; 40 gl(-1)). the 10 gl(-1) concentration was used to validate t ...201526378961
validation of a novel sequential cultivation method for the production of enzymatic cocktails from trichoderma strains.the development of new cost-effective bioprocesses for the production of cellulolytic enzymes is needed in order to ensure that the conversion of biomass becomes economically viable. the aim of this study was to determine whether a novel sequential solid-state and submerged fermentation method (sf) could be validated for different strains of the trichoderma genus. cultivation of the trichoderma reesei rut-c30 reference strain under sf using sugarcane bagasse as substrate was shown to be favorabl ...201525399068
secretomic survey of trichoderma harzianum grown on plant biomass substrates.the present work aims at characterizing t. harzianum secretome when the fungus is grown in synthetic medium supplemented with one of the four substrates: glucose, cellulose, xylan, and sugarcane bagasse (sb). the characterization was done by enzymatic assays and proteomic analysis using 2-de/maldi-tof and gel-free shotgun lc-ms/ms. the results showed that sb induced the highest cellulolytic and xylanolytic activities when compared with the other substrates, while remarkable differences in terms ...201424593137
transcriptome profile of trichoderma harzianum ioc-3844 induced by sugarcane bagasse.profiling the transcriptome that underlies biomass degradation by the fungus trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. in the present study, the transcriptome of t. harzianum ioc-3844 was analyzed using rna-seq technology. the sequencing generated 14.7 gbp for downstream analyses. de novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. ...201424558413
a novel promising trichoderma harzianum strain for the production of a cellulolytic complex using sugarcane bagasse in natura.brazil is a major producer of agro-industrial residues, such as sugarcane bagasse, which could be used as raw material for microbial production of cellulases as an important strategy for the development of sustainable processes of second generation ethanol production. for this purpose, this work aimed at screening for glycosyl hydrolase activities of fungal strains isolated from the brazilian cerrado. among 13 isolates, a trichoderma harzianum strain (l04) was identified as a promising candidate ...201324349958
understanding the cellulolytic system of trichoderma harzianum p49p11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-l-arabinofuranosidase.supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. this study investigated which enzymes were complementary to the enzyme set of trichoderma harzianum in the degradation of sugarcane bagasse. specific activities of t. harzianum extract on different substrates were compared with the extracts of penicillium echinulatum and trichoderma reesei, and two commercial cellulase preparations. comp ...201323391738
experimental mixture design as a tool to enhance glycosyl hydrolases production by a new trichoderma harzianum p49p11 strain cultivated under controlled bioreactor submerged fermentation.this work investigates the glycosyl hydrolase (gh) profile of a new trichoderma harzianum strain cultivated under controlled bioreactor submerged fermentation. the influence of different medium components (delignified steam-exploded sugarcane bagasse, sucrose, and soybean flour) on gh biosynthesis was assessed using experimental mixture design (emd). additionally, the effect of increased component concentrations in culture media selected from the emd was studied. it was found that that a mixed c ...201323265822
blue native-page analysis of trichoderma harzianum secretome reveals cellulases and hemicellulases working as multienzymatic complexes.plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergistically for polysaccharide degradation. here, we present evidence that the fungus trichoderma harzianum, cultivated in medium containing the agricultural residue sugarcane bagasse, is ...201222744980
high-yield endoglucanase production by trichoderma harzianum ioc-3844 cultivated in pretreated sugarcane mill byproduct.the low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. in the present paper, the filamentous fungus trichoderma harzianum ioc-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. this fungal strain produced high contents of endoglucanase acti ...201021048871
efficient isolation of anthraquinone-derivatives from trichoderma harzianum ets 323.anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from trichoderma harzianum ets 323. the fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. structure of chrysophanol was solved by x-ray diffraction and pachybasin by nmr spectra. about 233+/-13 mg of pure chrysophanol and 773+/-40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7+/ ...200717067682
production of a generic microbial feedstock for lignocellulose biorefineries through sequential bioprocessing.lignocellulosic materials, mostly from agricultural and forestry residues, provide a potential renewable resource for sustainable biorefineries. reducing sugars can be produced only after a pre-treatment stage, which normally involves chemicals but can be biological. in this case, two steps are usually necessary: solid-state cultivation of fungi for deconstruction, followed by enzymatic hydrolysis using cellulolytic enzymes. in this research, the utilisation of solid-state bioprocessing using th ...201728013134
process optimization and production kinetics for cellulase production by trichoderma viride vkf3.microbial cellulases are the enzymes widely studied due to their enormous applications in biochemical industry. among 12 fungal isolates isolated from mangrove plant debris and soil sample collected from valanthakad mangroves, kerala, india, 3 of them were found to exhibit cellulolytic activity. among them, the most potent isolate which exhibited maximum cellulolytic activity was identified as trichoderma viride vkf3 [gene bank accession number- jx683684.1] based on colony morphology, microscopi ...201424600546
enhanced cellulase production by trichoderma viride in a rotating fibrous bed bioreactor.filamentous fungi are widely used to produce cellulase, but how the fermentation conditions affect their production is not well known. in this study, cellulase production by trichoderma viride in submerged fermentations with free cells in a stirred-tank reactor (str) and immobilized cells in a rotating fibrous-bed bioreactor (rfbb) were investigated. compared to free-cell fermentation, immobilized-cell fermentation gave 35.5% higher fpase activity and 69.7% higher saccharification yield of sugar ...201323428816
first report of trypanosoma vivax outbreak in dairy cattle in são paulo state, brazil.this is the first description of a trypanosoma vivax outbreak in the state of são paulo (municipality of lins). fever, jaundice, decreased milk production, weight loss, profuse diarrhea, abortion, anemia, leukocytosis and hyperfibrinogenemia were observed in the affected animals. thirty-one cows and calves died out of a total of 1080 in the herd. three cows showed neurological symptoms like dysmetria, ataxia, muscle weakness, ptyalism, lymph node enlargement and submandibular edema. flagellated ...201322832751
surface polysaccharides and quorum sensing are involved in the attachment and survival of xanthomonas albilineans on sugarcane leaves.xanthomonas albilineans, the causal agent of sugarcane leaf scald, is a bacterial plant pathogen that is mainly spread by infected cuttings and contaminated harvesting tools. however, some strains of this pathogen are known to be spread by aerial means and are able to colonize the phyllosphere of sugarcane before entering the host plant and causing disease. the objective of this study was to identify the molecular factors involved in the survival or growth of x. albilineans on sugarcane leaves. ...201625962850
the gyrase inhibitor albicidin consists of p-aminobenzoic acids and cyanoalanine.albicidin is a potent dna gyrase inhibitor produced by the sugarcane pathogenic bacterium xanthomonas albilineans. here we report the elucidation of the hitherto unknown structure of albicidin, revealing a unique polyaromatic oligopeptide mainly composed of p-aminobenzoic acids. in vitro studies provide further insights into the biosynthetic machinery of albicidin. these findings will enable structural investigations on the inhibition mechanism of albicidin and its assessment as a highly effecti ...201525599532
breaking dogmas: the plant vascular pathogen xanthomonas albilineans is able to invade non-vascular tissues despite its reduced genome.xanthomonas albilineans, the causal agent of sugarcane leaf scald, is missing the hrp type iii secretion system that is used by many gram-negative bacteria to colonize their host. until now, this pathogen was considered as strictly limited to the xylem of sugarcane. we used confocal laser scanning microscopy, immunocytochemistry and transmission electron microscopy (tem) to investigate the localization of x. albilineans in diseased sugarcane. sugarcane plants were inoculated with strains of the ...201424522883
xanthomonas albilineans ompa1 appears to be functionally modular and both the omc and c-like domains are necessary for leaf scald disease of sugarcane.several ez-tn5 insertions in gene locus xalc_0557 (ompa1) of the sugarcane leaf scald pathogen xanthomonas albilineans xafl07-1 were previously found to strongly affect pathogenicity and endophytic stalk colonization. xalc_0557 has a predicted ompa n-terminal outer membrane channel (omc) domain and an ompa c-like domain. further analysis of mutant m468, with an ez-tn5 insertion in the upstream omc domain coding region, revealed impaired epiphytic and endophytic leaf survival, impaired resistance ...201323758144
genome sequence of xanthomonas sacchari r1, a biocontrol bacterium isolated from the rice seed.xanthomonas sacchari, was first identified as a pathogenic bacterium isolated from diseased sugarcane in guadeloupe. in this study, r1 was first isolated from rice seed samples from philippines in 2002. the antagonistic ability against several rice pathogens raises our attention. the genomic feature of this strain was described in this paper. the total genome size of x. sacchari r1 is 5,000,479 bp with 4315 coding sequences (cds), 59 trnas, 2rrnas and one plasmid.201525931193
draft genome sequence of xanthomonas sacchari strain lmg 476.we report the high-quality draft genome sequence of xanthomonas sacchari strain lmg 476, isolated from sugarcane. the genome comparison of this strain with a previously sequenced x. sacchari strain isolated from a distinct environmental source should provide further insights into the adaptation of this species to different habitats and its evolution.201525792064
detection of puccinia kuehnii causing sugarcane orange rust with a loop-mediated isothermal amplification-based assay.puccinia kuehnii is a fungal pathogen that causes orange rust in sugarcane, which is now prevalent in many countries. at the early stage of disease, it is almost indistinguishable from brown rust, which is caused by puccinia melanocephala. although several pcr assays are available to detect these diseases, the loop-mediated isothermal amplification (lamp)-based assay has been reported to be more economical and easier to perform. under isothermal conditions, dna is amplified with high specificity ...201626837389
puccinia kuehnii (krueg.) butler on sugarcane in india. 195015427344
genetic diversity among puccinia melanocephala isolates from brazil assessed using simple sequence repeat markers.brown rust (causal agent puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. despite its importance, little is known regarding the genetic diversity of this pathogen in the main brazilian sugarcane cultivation areas. in this study, we characterized the genetic diversity of 34 p. melanocephala isolates from 4 brazilian states using loci identified from an enriched simple sequence repeat (ssr) library. the aggressiveness of 3 isolates ...201425299099
pyraclostrobin wash-off from sugarcane leaves and aerobic dissipation in agricultural soil.to mitigate damage from the fungal sugarcane pathogen brown rust (puccinia melanocephala), a section 18 emergency use label was put in place by the united states environmental protection agency (u.s. epa) for the application of pyraclostrobin (trade name headline sc, produced by basf, research triangle park, nc) on sugarcane in 2008. to assess the dynamics of this fungicide in louisiana soil, samples (n = 24) from a non-treated field were spiked with pyraclostrobin (3.1 μg g(-1)) and analyzed in ...201424533668
haplotype structure around bru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars.modern sugarcane cultivars (saccharum spp., 2n = 100-130) are high polyploid, aneuploid and of interspecific origin. a major gene (bru1) conferring resistance to brown rust, caused by the fungus puccinia melanocephala, has been identified in cultivar r570. we analyzed 380 modern cultivars and breeding materials covering the worldwide diversity with 22 molecular markers genetically linked to bru1 in r570 within a 8.2 cm segment. our results revealed a strong ld in the bru1 region and strong assoc ...201222572763
the mating-type locus b of the sugarcane smut sporisorium scitamineum is essential for mating, filamentous growth and pathogenicity.sporisorium scitamineum is the causal agent of sugarcane smut, which is one of the most serious constraints to global sugarcane production. s. scitamineum and ustilago maydis are two closely related smut fungi, that are predicted to harbor similar sexual mating processes/system. to elucidate the molecular basis of sexual mating in s. scitamineum, we identified and deleted the ortholog of mating-specific u. maydis locus b, in s. scitamineum. the resultant b-deletion mutant was defective in mating ...201626563415
identification of putative candidate genes for red rot resistance in sugarcane (saccharum species hybrid) using ld-based association mapping.red rot is a serious disease of sugarcane caused by the fungus colletotrichum falcatum that has a colossal damage potential. the fungus, prevalent mainly in the indian sub-continent, keeps on producing new pathogenic strains leading to breakdown of resistance in newly released varieties and hence the deployment of linked markers for marker-assisted selection for resistance to this disease can fine tune the breeding programme. this study based on a panel of 119 sugarcane genotypes fingerprinted f ...201626961118
draft genome sequence of colletotrichum falcatum - a prelude on screening of red rot pathogen in sugarcane.colletotrichum falcatum, a concealed fungal ascomycete causes red rot, which is a serious disease in sugarcane. it infects economically important stalk tissues, considered as store house of sugar in sugarcane. the study is to find genetic complexities of c. falcatum in establishing this as a stalk infecting pathogen and to decipher the unique lifestyle of this pathogen using ngs technology. we report the draft genome of c. falcatum of about 48.16 mb in size with 12,270 genes. the genome sequence ...201626958090
molecular profiling of systemic acquired resistance (sar)-responsive transcripts in sugarcane challenged with colletotrichum falcatum.red rot disease of sugarcane caused by colletotrichum falcatum is one of the serious constraints affecting the productivity of the crop. the strategy of employing systemic acquired resistance (sar) against red rot yielded consistently good results at field level. however, elucidation of genes involved in the induction of sar continues to be a challenging area of research for a critical understanding of red rot disease resistance in sugarcane. here, temporal expression of 22 putative defense-rela ...201425234394
differential regulation of defense-related gene expression in response to red rot pathogen colletotrichum falcatum infection in sugarcane.red rot is a serious disease of sugarcane caused by the fungus colletotrichum falcatum imposing a considerable economic loss annually in all sugarcane-producing countries. in this study, we analyzed the early resistance response of sugarcane to red rot fungus by comparing the differences between control and inoculated stalk tissues. differential display reverse transcription polymerase chain reaction (dd-rt-pcr) was employed to identify altered expression of genes in disease-resistant cv co 9300 ...201323861092
optimization of β-glucosidase, β-xylosidase and xylanase production by colletotrichum graminicola under solid-state fermentation and application in raw sugarcane trash saccharification.efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. the production of β-glucosidase, β-xylosidase and xylanase by colletotrichum graminicola was optimized using response surface methodology (rsm). maximal production occurred in wheat bran. sugarcane trash, peanut hulls and corncob enhanced β-glucosidase, β-xylosidase and xylanase production, respectively. maximal levels after optimization reached 159.3 ± 12.7 u g-1, 128.1 ...201323364611
use of simple sequence repeat markers for dna fingerprinting and diversity analysis of sugarcane (saccharum spp) cultivars resistant and susceptible to red rot.red rod is an economically important disease of sugarcane caused by the fungus colletotrichum falcatum. we used a simple sequence repeat (ssr)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in pakistan. twenty-one highly polymorphic ssr markers were used for dna fingerprinting and genetic diversity analysis of 20 sugarcane cultivars. these ssr markers were found to be highly robust; we identified 144 alleles, with ...201222614346
unraveling evolutionary relationships among the divergent lineages of colletotrichum causing anthracnose disease in turfgrass and corn.abstract colletotrichum species cause anthracnose diseases on a number of grass hosts and are common inhabitants of many others. they are divided into four species: c. sublineolum is pathogenic to sorghum spp.; c. caudatum is found on c4 grasses such as indiangrass and big bluestem; c. falcatum causes red rot of sugarcane; and c. graminicola sensu lato is a broadly defined species including isolates that attack maize, wheat, oats, and many forage, turf, and amenity grasses of the subfamily pooid ...200618944204
insect and plant-derived mirnas in greenbug (schizaphis graminum) and yellow sugarcane aphid (sipha flava) revealed by deep sequencing.schizaphis graminum (green bug; gb) and sipha flava (yellow sugarcane aphid; ysa) are two cereal aphid species with broad host ranges capable of establishing on sorghum (sorghum bicolor) and several switchgrass (panicum virgatum) cultivars. switchgrass and sorghum are staple renewable bioenergy crops that are vulnerable to damage by aphids, therefore, identifying novel targets to control aphids has the potential to drastically improve yields and reduce losses in these bioenergy crops. despite th ...201727838454
short and long-term antinutritional effect of the trypsin inhibitor apti for biological control of sugarcane borer.plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. in this work, we tested the anti-insect activity of adenanthera pavonina trypsin inhibitor (apti) against diatraea saccharalis larvae, a major insect pest in sugarcane. the addition of 0.1% apti in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. apti was not digested after 60h incubation with ...201424355140
[control discourses and power relations of yellow fever: philadelphia in 1793].1793 yellow fever in philadelphia was the most severe epidemics in the late 18th century in the united states. more than 10% of the population in the city died and many people fled to other cities. the cause of yellow fever in the united states had close relationship with slaves and sugar in philadelphia. sugarcane plantation had needed many labors to produce sugar and lots of africans had to move to america as slaves. in this process, aëdes aegypti, the vector of yellow fever had migrated to am ...201425608507
defense response in non-genomic model species: methyl jasmonate exposure reveals the passion fruit leaves' ability to assemble a cocktail of functionally diversified kunitz-type trypsin inhibitors and recruit two of them against papain.multiplicity of protease inhibitors induced by predators may increase the understanding of a plant's intelligent behavior toward environmental challenges. information about defense mechanisms of non-genomic model plant passion fruit (passiflora edulis sims) in response to predator attack is still limited. here, via biochemical approaches, we showed its flexibility to build-up a broad repertoire of potent kunitz-type trypsin inhibitors (ktis) in response to methyl jasmonate. seven inhibitors (20- ...201424849173
complete chloroplast genome sequences of hordeum vulgare, sorghum bicolor and agrostis stolonifera, and comparative analyses with other grass genomes.comparisons of complete chloroplast genome sequences of hordeum vulgare, sorghum bicolor and agrostis stolonifera to six published grass chloroplast genomes reveal that gene content and order are similar but two microstructural changes have occurred. first, the expansion of the ir at the ssc/ira boundary that duplicates a portion of the 5' end of ndhh is restricted to the three genera of the subfamily pooideae (agrostis, hordeum and triticum). second, a 6 bp deletion in ndhk is shared by agrosti ...200717534593
using exploratory factor analysis of ffq data to identify dietary patterns among yup'ik people.an ffq developed by the center for alaska native health research for studies in yup'ik people includes market foods and subsistence foods such as moose, seal, waterfowl and salmon that may be related to disease risk. because the ffq contains >100 food items, we sought to characterize dietary patterns more simply for use in ongoing pharmacogenomics studies.201423290469
toxicological effects of the waste of the sugarcane industry, used as agricultural fertilizer, on the test system allium cepa.sugarcane is cultivated in tropical countries for sugar and ethanol production. in brazil, this culture is among the most profitable with a production of 658.7 million tons/harvest. sugarcane filter cake (scfc) is a waste rich in organic matter and micronutrients, but also contains toxic metals. as it has been used as fertilizer and there is not enough knowledge about its environmental impacts, this work assessed the genotoxicogenetic effects of raw scfc and associations with soil and sugarcane ...201728104478
adsorption of pharmaceuticals in water through lignocellulosic fibers synergism.the contamination of water from disposal of drugs is an emerging problem due to their consequences on trophic webs. this study evaluated the ability of sugarcane and coconut fiber to reduce water toxicity contaminated by pharmaceuticals. the toxicity of solutions containing pharmaceuticals was studied by bioassay using allium cepa, before and after filtration of contaminated water. the coconut and sugarcane fiber have not been satisfactory in reducing toxicity when tested separately. despite no ...201728002767
allium cepa and tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid.the indiscriminate use of pesticides has become a serious environmental concern. of them, imidacloprid (imi) is one of the most widely used worldwide. in 2010 in brazil, 1.934 tonnes of imi were sold and mainly used for sugarcane crops. several studies have examined the toxicity of imi as well as its possible ecological effects. however, few studies have examined its toxicity at the genetic level. this is one of the biggest challenges for the scientific community, which is concerned about the im ...201525225953
harvest time residues of pendimethalin and oxyfluorfen in vegetables and soil in sugarcane-based intercropping systems.terminal residues of pendimethalin and oxyfluorfen applied in autumn sugarcane- and vegetables-based intercropping systems were analyzed in peas (pisum sativum), cabbage (brassica oleracea), garlic (allium sativum), gobhi sarson (brassica napus), and raya (brassica juncea). the study was conducted in winter season in 2010-2011 and in 2011-2012 at ludhiana, india. pendimethalin at 0.56 kg and 0.75 kg ha(-1) was applied immediately after sowing of gobhi sarson, raya, peas, garlic, and 2 days befor ...201525832010
comparison between brazilian agro-wastes and activated carbon as adsorbents to remove ni(ii) from aqueous solutions.this research was performed to find an alternative, low-cost, competitive, locally available and efficient adsorbent to treat nickel (ni) containing effluents. for this purpose, several brazilian agro-wastes like sugarcane bagasse (scb), passion fruit wastes (pfw), orange peel (op) and pineapple peel (pp) were compared with an activated carbon (ac). the adsorbents were characterized. effects of fundamental factors affecting the adsorption were investigated using batch tests. kinetic and equilibr ...201627232408
interaction of polyphenol oxidase of solanum tuberosum with β-cyclodextrin: process details and applications.polysaccharides differing in structure and chemical nature were screened for their ability to bind non-covalently with polyphenol oxidase (ppo) from potato (as a model) and their effect on enzyme activity. all the polysaccharides selected inhibited the ppo but β-cyclodextrin showed maximum inhibition under optimum conditions. process details for the inhibition of ppo were studied with respect to concentration of β-cyclodextrin, temperature, ph, and time. higher inhibition constant and lower half ...201526187193
cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials.according to the cost of carbon substrate for producing biopolymer of polyhydroxyalkanoates (phas) still has a barrier to extend in an industrial scale. the aim of this work was to evaluate the defined phas media containing the agricultural raw materials of pineapple and sugarcane to produce phas by bacillus strain. batch fermentation was carried out in flask scale to compare the efficiency of defined media using statistical methodology. the defined medium 8 of pineapple was achieved the highest ...201526185927
utilization of agricultural residues of pineapple peels and sugarcane bagasse as cost-saving raw materials in scenedesmus acutus for lipid accumulation and biodiesel production.the aim of this study is to optimize the lipid accumulation in microalgae by using two agricultural residues of pineapple peels and sugarcane bagasse as low-cost organic carbon sources. green microalgae scenedesmus acutus was isolated and selected for cultivation. effects of three initial sugar concentrations and the stage for adding sugar during cultivation on biomass and lipid production were investigated. the results clearly showed that two-stage cultivation is more suitable than one-stage. t ...201424817554
the influence of total solids content and initial ph on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes.hydrogen is a valuable clean energy source, and its production by biological processes is attractive and environmentally sound and friendly. in méxico 5 million tons/yr of agroindustrial wastes are generated; these residues are rich in fermentable organic matter that can be used for hydrogen production. on the other hand, batch, intermittently vented, solid substrate fermentation of organic waste has attracted interest in the last 10 years. thus the objective of our work was to determine the eff ...201323732191
rna interference of endochitinases in the sugarcane endophyte trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.the sugarcane root endophyte trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including n-acetyl-ß-d-glucosaminidases, chitobiosidases and endochitinases. we used targeted gene deletion and rna-dependent gene silencing strategies to disrupt n-acetyl-ß-d-glucosaminidase and endochitinase activities of the ...201223110120
occupational exposure to pesticides and parkinson's disease: a systematic review and meta-analysis of cohort studies.to systematically review available cohort studies and estimate quantitatively the association between occupational exposure to pesticides and parkinson's disease (pd).201222698719
chemical shift assignments of the canecystatin-1 from saccharum officinarum.cystatins are cysteine proteases inhibitors that are widely distributed among insects, mammalians and plants. here we report the complete resonance assignment of canecystatin-1 from saccharum officinarum obtained by heteronuclear multidimensional high-resolution nuclear magnetic resonance spectroscopy. the consensus chemical shift index was calculated and showed the presence of one α-helix (residues 27-43) and three β-strands (residues 48-74, 78-89 and 94-104), a secondary structure pattern that ...201322696137
in vivo genotoxicity evaluation of atrazine and atrazine-based herbicide on fish carassius auratus using the micronucleus test and the comet assay.atrazine is a selective triazine herbicide used to control broadleaf and grassy weeds mainly in corn, sorghum, sugarcane, pineapple, and other crops, and in conifer reforestation planting fields. it has been showed that atrazine is one of the most frequently detected pesticides in agricultural streams and rivers, over the past two decades. although the toxic properties of atrazine are well known, the data on the genotoxic effects of atrazine on aquatic organisms are rather scarce. thus, in the p ...201121443921
screening of trichoderma isolates as a biological control agent against ceratocystis paradoxa causing pineapple disease of sugarcane.in this study, dual culture, poison agar, and direct methods were used to assess the ability of trichoderma virens imi-392430, t. pseudokoningii imi-392431, t. harzianum imi-392432, t. harzianum imi-392433, and t. harzianum imi-392434 to control ceratocystis paradoxa, which causes the pineapple disease of sugarcane. the highest percentage inhibition of radial growth (pirg) values were observed with t. harzianum imi-392432 using two dual culture methods, 63.80% in method i and 80.82% in method ii ...200923983548
biofibers from agricultural byproducts for industrial applications.lignocellulosic agricultural byproducts are a copious and cheap source for cellulose fibers. agro-based biofibers have the composition, properties and structure that make them suitable for uses such as composite, textile, pulp and paper manufacture. in addition, biofibers can also be used to produce fuel, chemicals, enzymes and food. byproducts produced from the cultivation of corn, wheat, rice, sorghum, barley, sugarcane, pineapple, banana and coconut are the major sources of agro-based biofibe ...200515629854
tropgene-db, a multi-tropical crop information system.tropgene-db, is a crop information system created to store genetic, molecular and phenotypic data of the numerous yet poorly documented tropical crop species. the most common data stored in tropgene-db are information on genetic resources (agro-morphological data, parentages, allelic diversity), molecular markers, genetic maps, results of quantitative trait loci analyses, data from physical mapping, sequences, genes, as well as the corresponding references. tropgene-db is organized on a crop bas ...200414681435
sugarcane workers: morbidity and mortality.sugarcane is, after pineapple, the largest agricultural industry in hawaii. there have been reports that this industry poses certain health hazards. to investigate this possible hazard in hawaii, the relationship of employment on a sugarcane plantation to total mortality, the development of definite coronary heart disease (chd), stroke, cancer, lung cancer and certain risk factors were examined in men of japanese ancestry participating in the honolulu heart program. after 18 years of follow-up, ...19938300403
photosynthetic pathway and biomass energy production.the current interest in locating new or alternative sources of energy has focused attention on solar energy capture by crops that can be subsequently utilized as a substitute for fossil fuels. the very high productivity of sugarepane and the fact that it accumulates sugars that are directly fermentable to alcohol may have caused seemingly less productive crops to be overlooked. we show here that recoverable alcohol from achievable commercial yields of pineapple can actually equal that of sugarca ...197917729660
evaluation of in vitro and in vivo effects of semipurified proteinase inhibitors from theobroma seeds on midgut protease activity of lepidopteran pest insects.we have characterized in vitro and in vivo effects of trypsin inhibitors from theobroma seeds on the activity of trypsin- and chymotrypsin-like proteins from lepidopteran pest insects. the action of semipurified trypsin inhibitors from theobroma was evaluated by the inhibition of bovine trypsin and chymotrypsin activities determined by the hydrolysis of n-benzoyl-dl-arginine-p-nitroanilide (bapa) and n-succinyl-ala-ala-pho-phe p-nitroanilide (s-(ala)2prophe-pna). proteinase inhibitor activities ...201222806759
moisture source and diet affect development and reproduction of orius thripoborus and orius naivashae, two predatory anthocorids from southern africa.the effect of moisture source and diet on the development and reproduction of the pirate bugs, orius thripoborus (hesse) and orius naivashae (poppius) (hemiptera: anthocoridae) was examined in the laboratory. both species had been collected in and around sugarcane fields in south africa. supplementing eggs of the flour moth ephestia kuehniella (zeller) (lepidoptera: pyralidae) with a green bean pod as a moisture source yielded better nymphal survival and faster development, as compared with free ...201222935002
effects of food processing and fibre content on the digestibility, energy intake and biochemical parameters of blue-and-gold macaws (ara ararauna l. - aves, psittacidae).considering the increased incidence of obesity and metabolic diseases in caged psittacines, the effect of fibre and food processing was evaluated in the blue-and-gold macaw. four food formulations (0%, 7%, 14% and 21% of sugarcane fibre) processed by pelleting or extrusion were studied, resulting in eight diets. to study digestibility, 48 macaws housed in pairs in cages was used in a block design. subsequently, diets containing 0% or 21% sugarcane fibre, pelleted or extrude was fed for 4 months ...201423627662
expression of arabidopsis bax inhibitor-1 in transgenic sugarcane confers drought tolerance.the sustainability of global crop production is critically dependent on improving tolerance of crop plants to various types of environmental stress. thus, identification of genes that confer stress tolerance in crops has become a top priority especially in view of expected changes in global climatic patterns. drought stress is one of the abiotic stresses that can result in dramatic loss of crop productivity. in this work, we show that transgenic expression of a highly conserved cell death suppre ...201626872943
plant transcriptomics and responses to environmental stress: an overview.different stresses include nutrient deficiency, pathogen attack, exposure to toxic chemicals etc. transcriptomic studies have been mainly applied to only a few plant species including the model plant, arabidopsis thaliana. these studies have provided valuable insights into the genetic networks of plant stress responses. transcriptomics applied to cash crops including barley, rice, sugarcane, wheat and maize have further helped in understanding physiological and molecular responses in terms of ge ...201526440096
the core root microbiome of sugarcanes cultivated under varying nitrogen fertilizer application.diazotrophic bacteria potentially supply substantial amounts of biologically fixed nitrogen to crops, but their occurrence may be suppressed by high nitrogen fertilizer application. here, we explored the impact of high nitrogen fertilizer rates on the presence of diazotrophs in field-grown sugarcane with industry-standard or reduced nitrogen fertilizer application. despite large differences in soil microbial communities between test sites, a core sugarcane root microbiome was identified. the sug ...201626032777
reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes.polyhydroxyalkanoates (phas) are bacterial carbon storage polymers used as renewable, biodegradable plastics. pha production in plants may be a way to reduce industrial pha production costs. we recently demonstrated a promising level of peroxisomal pha production in the high biomass crop species sugarcane. however, further production strategies are needed to boost pha accumulation closer to commercial targets. through exogenous fatty acid feeding of arabidopsis thaliana plants that contain perox ...201424944109
induced over-expression of atdreb2a ca improves drought tolerance in sugarcane.drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and, in some cases, yield losses caused by drought are nearly 50%. dreb proteins play vital regulatory roles in abiotic stress responses in plants. the transcription factor dreb2a interacts with a cis-acting dre sequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in arabidopsis thaliana. in the present study, we evaluated the effec ...201424656336
circadian rhythms of sense and antisense transcription in sugarcane, a highly polyploid crop.commercial sugarcane (saccharum hybrid) is a highly polyploid and aneuploid grass that stores large amounts of sucrose in its stem. we have measured circadian rhythms of sense and antisense transcription in a commercial cultivar (rb855453) using a custom oligoarray with 14,521 probes that hybridize to sense transcripts (ss) and 7,380 probes that hybridize to antisense transcripts (as).we estimated that 32% of ss probes and 22% as probes were rhythmic. this is a higher proportion of rhythmic prob ...201323936527
enzymatic activity and proteomic profile of class iii peroxidases during sugarcane stem development.class iii peroxidases are present as large multigene families in all land plants. this large number of genes together with the diversity of processes catalyzed by peroxidases suggests possible functional specialization of each isoform. however, assigning a precise role for each individual peroxidase gene has continued to be a major bottleneck. here we investigated the enzyme activity and translational profile of class iii peroxidases during stem development of sugarcane as a first step in the es ...201222551762
dissecting the genome of the polyploid crop oilseed rape by transcriptome sequencing.polyploidy complicates genomics-based breeding of many crops, including wheat, potato, cotton, oat and sugarcane. to address this challenge, we sequenced leaf transcriptomes across a mapping population of the polyploid crop oilseed rape (brassica napus) and representative ancestors of the parents of the population. analysis of sequence variation and transcript abundance enabled us to construct twin single nucleotide polymorphism linkage maps of b. napus, comprising 23,037 markers. we used these ...201121804563
c4gem, a genome-scale metabolic model to study c4 plant metabolism.leaves of c(4) grasses (such as maize [zea mays], sugarcane [saccharum officinarum], and sorghum [sorghum bicolor]) form a classical kranz leaf anatomy. unlike c(3) plants, where photosynthetic co(2) fixation proceeds in the mesophyll (m), the fixation process in c(4) plants is distributed between two cell types, the m cell and the bundle sheath (bs) cell. here, we develop a c(4) genome-scale model (c4gem) for the investigation of flux distribution in m and bs cells during c(4) photosynthesis. c ...201020974891
expression analysis of sugarcane shaggy-like kinase (susk) gene identified through cdna subtractive hybridization in sugarcane (saccharum officinarum l.).identification of genes whose expression enables plants to adapt to any kind of stresses is integral to developing stress tolerance in crop plants. in this study, pcr-based cdna suppression subtractive hybridization technique was used to construct sugarcane salt (nacl) stress specific forward and reverse subtracted cdna library. for this, mrnas were pooled from the shoot and root tissues stressed with nacl (200 mm) for various time intervals (0.5 to 18 h). sequencing the clones from the forward ...201120853012
expression and variability of molecular chaperones in the sugarcane expressome.molecular chaperones perform folding assistance in newly synthesized polypeptides preventing aggregation processes, recovering proteins from aggregates, among other important cellular functions. thus their study presents great biotechnological importance. the present work discusses the mining for chaperone-related sequences within the sugarcane est genome project database, which resulted in approximately 300 different sequences. since molecular chaperones are highly conserved in most organisms s ...200716687190
the plant energy-dissipating mitochondrial systems: depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots.the simultaneous existence of alternative oxidases and uncoupling proteins in plants has raised the question as to why plants need two energy-dissipating systems with apparently similar physiological functions. a probably complete plant uncoupling protein gene family is described and the expression profiles of this family compared with the multigene family of alternative oxidases in arabidopsis thaliana and sugarcane (saccharum sp.) employed as dicot and monocot models, respectively. in total, s ...200616473895
characterization of cenh3 and centromere-associated dna sequences in sugarcane.centromere-specific histone h3 (cenh3) has been used to detect active centromeres, and to analyse the dna sequences closely associated with the centromere, because they localize only in active centromeres and bind directly to the dna. in maize and rice, the centromeric retrotransposons (cr) are shown to be closely associated with their own cenh3 whereas no such association was found in arabidopsis thaliana. in this study, this sort of association was investigated in sugarcane. two expressed sequ ...200515861308
[cloning and sequencing of acc oxidase gene from sugarcane].the plant hormone ethylene is not only responsible for the initiation of fruit ripening, senescence and dormancy but also for regulating many other plant developmental processes, such as seed germination, root initiation, growth, floral differentiation, sex differentiation and responding to environment stresses. one of the rate-limiting steps for ethylene biosynthesizing in plant is catalyzed by 1-aminocyclopropane-1-carboxylate (acc) oxidase. understanding of ethylene expressive pattern in plan ...200312812078
impact of filter mud applications on the germination of sugarcane and small-seeded plants and on soil and sugarcane nitrogen contents.the impact of filter mud (fm) on the germination of sugarcane (saccharum spp.), guar (cyamopsis tetragonoloba), maize (zea mays), groundnut (arachis hypogaea) and sesame (sesamum indicum), and the nitrogen content of soil and sugarcane were investigated. experiments were set up in 2004 at the kenana sugarcane estate, sudan. for this study, 1:0,3:1,1:1,1:3 and 0:1 fm/soil (w/w) mixtures were used. the results showed that fm had no adverse impact on germination. soil organic carbon and total nitro ...200817945488
abiotic limits for germination of sugarcane seed in relation to environmental spread.sugarcane is a vegetatively propagated crop and hence the production of seed and its fate in the environment has not been studied. the recent development of genetically modified sugarcane, with the aim of commercial production, requires a research effort to understand sugarcane reproductive biology. this study contributes to this understanding by defining the abiotic limits for sugarcane seed germination. using seed from multiple genetic crosses, germination was measured under different light re ...201425485029
[differences in the light-activation of nadp-dependent glyceraldehyde-3-phosphate dehydrogenase and of ribulose-5-phosphate kinase between plants containing the calvin and those containing the c4-dicarboxylic acid pathway of photosynthetic carbon reduction].1. preceding experiments had shown that irradiance of intact leaves or of isolated chloroplasts causes a reversible increase in the activity of nadp-gpd (ziegler and ziegler, 1965) as well as of ribulose-5-phosphate kinase (latzko and gibbs, 1969). examination of several species which carry out the calvin type of photosynthetic co2 fixation (vicia faba, spinacia oleracea, nicotiana tabacum, avena sativa) now revealed that the dark level of nadp-gpd activity ranges between 300-400 μmol nadph/mg c ...197124493083
the treatment of jaundice with medicinal plants in indigenous communities of the sub-himalayan region of uttarakhand, india.inspite of tremendous advances made in allopathic medical practices, herbs still play an important role in the management of various liver diseases. a large number of plants and formulations have been claimed to have hepatoprotective activity. jaundice is a symptom, indicative of the malfunctioning of the liver. this paper provides ethnomedicinal information on the plants used to treat jaundice by three important indigenous communities, i.e., nomadic gujjars, tharu and bhoxa of sub-himalayan reg ...201222759701
critical electrolyte concentration of silk gland chromatin of the sugarcane borer diatraea saccharalis, induced using agrochemicals.the sugarcane borer diatraea saccharalis is widely known as the main pest of sugarcane crop, causing increased damage to the entire fields. measures to control this pest involve the use of chemicals and biological control with cotesia flavipes wasps. in this study, we evaluated the insecticides fipronil (frontline; 0.0025%), malathion (malatol bio carb; 0.4%), cipermetrina (galgotrin; 10%), and neem oil (natuneem; 100%) and the herbicide nicosulfuron (sanson 40 sc; 100%) in the posterior region ...201425299111
use of plant residues on growth of mycorrhizal seedlings of neem (azadirachta indica a. juss.).owing to its multiple uses in veterinary medicine, biofertilizers, pest control, etc., the commercial cultivation of neem (azadirachta indica) has been increasing in various countries. the use of arbuscular mycorrhizal fungi (amf) and plant by-products (composted leaves and residues of neem and sugarcane) for the propagation of seedlings can be an efficient alternative to stimulate plant growth, reducing the propagation time and conferring increased tolerance of plants to biotic and abiotic stre ...201225363647
bioactivity of neem, azadirachta indica, against spittlebug mahanarva fimbriolata (hemiptera: cercopidae) on sugarcane.the effect of neem, azadirachta indica a. juss, on some biological parameters of mahanarva fimbriolata (stil) (hemiptera: cercopidae) was studied in the laboratory by using neemazal-t/s, nimkol-ls, and an aqueous neem seed extract. initially, the lc,, was estimated for nymphs. later, nymphs fed on sugarcane, saccharum officinarum l., roots were sprayed with the respective lc,, for each product. the adults were maintained in cages on sugarcane plants sprayed at the base with the maximum rate reco ...200617195667
centesimal composition and physical-chemistry analysis of the edible mushroom lentinus strigosus occurring in the brazilian amazon.the centesimal composition and the physical and chemical analyses of lentinus strigosus, an edible mushroom occurring in the brazilian amazon and produced in alternative substrates based on wood and agroindustrial residues, were evaluated. for this purpose, the c, n, ph, soluble solids, water activity, protein, lipids, total fiber, ash, carbohydrate, and energy levels were determined. the substrates were formulated from simarouba amara aubl. ("marupá"), ochroma piramidale cav. ex. lam. ("pau-de- ...201324141410
new species and new records of tetranychidae (acarina, prostigmata) from thailand.sampling efforts conducted in several provinces from thailand disclosed three new species of tetranychid mites. two of them belong to the genus tetranychus, namely tetranychus occultaspina sp. nov. and tetranychus truncatissimus sp. nov. and the third species belongs to the genus schizotetranychus, schizotetranychus krungthepensis sp. nov. they were collected on ipomoea aquatica, bambusa multiplex and saccharum officinarum, respectively. new records and new hosts are also mentioned.201424871006
microscopy characterization of silica-rich agrowastes to be used in cement binders: bamboo and sugarcane leaves.agrowastes are produced worldwide in huge quantities and they contain interesting elements for producing inorganic cementing binders, especially silicon. conversion of agrowastes into ash is an interesting way of yielding raw material used in the manufacture of low-co2 binders. silica-rich ashes are preferred for preparing inorganic binders. sugarcane leaves (saccharum officinarum, sl) and bamboo leaves (bambusa vulgaris, bvl and bambusa gigantea, bgl), and their corresponding ashes (sla, bvla, ...201526343378
recent trends in bioethanol production from food processing byproducts.the widespread use of corn starch and sugarcane as sources of sugar for the production of ethanol via fermentation may negatively impact the use of farmland for production of food. thus, alternative sources of fermentable sugars, particularly from lignocellulosic sources, have been extensively investigated. another source of fermentable sugars with substantial potential for ethanol production is the waste from the food growing and processing industry. reviewed here is the use of waste from potat ...201627565674
utilization of vinasses as soil amendment: consequences and perspectives.vinasses are a residual liquid generated after the production of beverages, such as mezcal and tequila, from agave (agave l.), sugarcane (saccharum officinarum l.) or sugar beet (beta vulgaris l.). these effluents have specific characteristics such as an acidic ph (from 3.9 to 5.1), a high chemical oxygen demand (50,000-95,000 mg l(-1)) and biological oxygen demand content (18,900-78,300 mg l(-1)), a high total solids content (79,000 and 37,500 mg l(-1)), high total volatile solids 79,000 and 82 ...201627441131
production of sugarcane bagasse-based activated carbon for formaldehyde gas removal from potted plants exposure chamber.agricultural wastes such as rice straw, sugar beet, and sugarcane bagasse have become a critical environmental issue due to growing agriculture demand. this study aimed to investigate the valorization possibility of sugarcane bagasse waste for activated carbon preparation. it also aimed to fully characterize the prepared activated carbon (bet surface area) via scanning electron microscope (sem) and in terms of surface functional groups to give a basic understanding of its structure and to study ...201526606041
roles of reversible and irreversible aggregation in sugar processing.colloids (1-1000-nm particles) in sugarcane/beet juice originate from non-sucrose impurities (polyphenolic colorants, residual soil, polysaccharides) of the plant materials; additional colloids form during the high temperature processing. colloids are reactive toward aggregation, sorption, desorption, and redox/hydrolysis/thermal transformation reactions. both derjaguin-landau-verwey-overbeek (dlvo; van der waals and electrostatic forces) and non-dlvo (involving hydrophilic colloids) interaction ...201726065543
unintended consequences of bioethanol feedstock choice in china.economic, energy, and environmental impacts of 11 types of bioethanol feedstock in china were evaluated using a mixed-unit input-output life cycle assessment model. corn grain and wheat grain had higher negative economic, energy, and environmental impacts. sweet sorghum, cassava, sugar beet, and sugarcane showed better economic performance but increasing negative energy and environmental impacts. cellulose-based feedstocks in general showed positive economic, energy, and environmental performanc ...201223070140
health impact assessment of liquid biofuel production.bioethanol and biodiesel as potential substitutes for fossil fuels in the transportation sector have been analyzed for environmental suitability. however, there could be impacts on human health during the production, therefore adverse health effects have to be analyzed. the aim of this study is to analyze to what health risk factors humans are exposed to in the production of biofuels and what the size of the health effects is. a health impact assessment expressed as disability adjusted life year ...201322774773
measurement and analysis of the mannitol partition coefficient in sucrose crystallization under simulated industrial conditions.mannitol is a major deterioration product of leuconstoc mesenteroides bacterial metabolism of sucrose and fructose from both sugarcane and sugar beet. the effect of crystallization conditions on the mannitol partition coefficient (k(eff)) between impure sucrose syrup and crystal has been investigated in a batch laboratory crystallizer and a batch pilot plant-scale vacuum pan. laboratory crystallization was operated at 65.5°c (150°f), 60.0°c (140°f), and 51.7°c (125°f) with a 78.0 brix (% refract ...201222632769
sucrose transporters of higher plants.recent advances have provided new insights into how sucrose is moved from sites of synthesis to sites of utilisation or storage in sink organs. sucrose transporters play a central role, as they orchestrate sucrose allocation both intracellularly and at the whole plant level. sucrose produced in mesophyll cells of leaves may be effluxed into the apoplasm of mesophyll or phloem parenchyma cells by a mechanism that remains elusive, but experimentally consistent with facilitated transport or energy- ...201020303321
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