| discovery of novel antimycobacterial drug therapy in biofilm of pathogenic nontuberculous mycobacterial keratitis. | the potential of slow-growing mycobacteria to form biofilms in human tissues contributes to the problem of establishing an effective treatment strategy. the purpose of this study was to examine new antibiotic strategies to enhance current treatment options for these infections. | 2017 | 28662943 |
| mycobacterium aquaticum sp. nov., a rapidly growing species isolated from haemodialysis water. | the characterization of five iranian isolates, four from hospital haemodialysis water and one from the sputum of a patient, led to the detection of a novel mycobacterium species. the strains were characterized by mucoid colonies developing in 3-5 days at temperatures ranging from 25 to 37 °c. the biochemical test pattern was unremarkable while the hplc profile of mycolic acids resembled that of mycobacterium fortuitum. the sequences of three major housekeeping genes (16s rrna, hsp65 and rpob) we ... | 2017 | 28829035 |
| efficacy of calcium hypochlorite and ultraviolet irradiation against mycobacterium fortuitum and mycobacterium marinum. | nontuberculous mycobacteria (ntm) cause opportunistic infections with increasing frequency in immunocompromised humans. water is one of the natural sources for transmission of ntm and plays a major role in the epidemiology of ntm infections. this study evaluated the efficacy of calcium hypochlorite and ultraviolet irradiation (uv) to eliminate potentially zoonotic ntm species such as m. marinum and m. fortuitum. | 2017 | 28776533 |
| arsenic exposure impels cd4 commitment in thymus and suppress t cell cytokine secretion by increasing regulatory t cells. | arsenic is globally infamous for inducing immunosuppression associated with prevalence of opportunistic infection in exposed population, although the mechanism remains elusive. in this study, we investigate the effect of arsenic exposure on thymocyte lineage commitment and the involvement of regulatory t cells (treg) in arsenic-induced immunosuppression. male balb/c mice were exposed to 0.038, 0.38 and 3.8 ppm sodium arsenite for 7, 15 and 30 days through oral gavage. arsenic exposure promoted c ... | 2017 | 28769045 |
| prosthetic valve infective endocarditis with mycobacterium fortuitum: antibiotics alone can be curative. | rapidly growing non-tuberculous mycobacteria (rgm) are a rare, often fatal cause of infective endocarditis. although surgery has been the cornerstone of rgm endocarditis therapy, we present the first documented adult case of mycobacterium fortuitum endocarditis cured with antibiotic therapy. | 2017 | 28688832 |
| antimicrobial susceptibility testing and molecular characterization of mycobacterium fortuitum isolates in china. | we performed molecular identification of clinical isolates of mycobacterium fortuitum (m. fortuitum) and conducted drug susceptibility testing to analyze the in vitro susceptibility of clinical m. fortuitum isolates and potential molecular mechanism conferring resistance to fluoroquinolone and macrolide drugs. the results showed that moxifloxacin had the highest in vitro activity against m. fortuitum, and most m. fortuitum isolates were resistant to clarithromycin and linezolid in china. the los ... | 2017 | 28549494 |
| draft genome sequence of mycobacterium boenickei cip 107829. | mycobacterium boenickei is a rapidly growing mycobacterium isolated for the first time from a leg wound in the united states. its 6,506,908-bp draft genome exhibits a 66.77% g+c content, 6,279 protein-coding genes, and 59 predicted rna genes. in silico dna-dna hybridization confirms its assignment to the mycobacterium fortuitum complex. | 2017 | 28473389 |
| septicemia and pneumonia due to mycobacterium fortuitum infection in a patient with extronodal nk/t-cell lymphoma, nasal type: a case report. | mycobacterium fortuitum (m.fortuitum) is one of the rapidly growing nontuberculous mycobacterium (ntm) that is widespread in the environment. m.fortuitum can cause different types of disease including pulmonary disease, lymphadenitis, cutaneous disease, and disseminated disease. however, the infection presenting as septicemia is exceedingly rare. | 2017 | 28471978 |
| mutual interaction enables the mycobacterial plasmid pal5000 origin binding protein repb to recruit repa, the plasmid replicase, to the origin. | the mycobacterium fortuitum plasmid, pal5000, is the most-studied member of a family of plasmids that are found in actinobacteria. its replication is brought about by the combined action of two plasmid-encoded replication proteins, repa and repb. repb has earlier been shown to be a sigma factor homologue that possesses origin-binding activity. the mechanism by which repa functions, and its relationship with repb, if any, has not been explored yet. in this study, we show that repa shares a common ... | 2017 | 28430099 |
| a strong and fortuitous case of dyspnea. | a 48 year-old man with no past medical history was sent to our emergency department (ed); from a primary care clinic for hypertensive urgency of 200/130. the man reported an intermittent non-productive cough of approximately one year's duration and worsening dyspnea on exertion and orthopnea over the last month with lower extremity swelling. of note, he emigrated from honduras twenty years ago. blood pressure normalized with administration of lasix in the ed. physical exam revealed rales in lung ... | 2017 | 28414672 |
| environmental screening of aeromonas hydrophila, mycobacterium spp., and pseudocapillaria tomentosa in zebrafish systems. | health monitoring systems are developed and used in zebrafish research facilities because pathogens of danio rerio such as aeromonas hydrophila, mycobacterium spp., and pseudocapillaria tomentosa have the potential to impair animal welfare and research. the fish are typically analyzed post mortem to detect microbes. the use of sentinels is a suggested way to improve the sensitivity of the surveillance and to reduce the number of animals to sample. the setting of a pre-filtration sentinel tank ou ... | 2017 | 29286459 |
| amplification of hsp 65 gene and usage of restriction endonuclease for identification of non tuberculous rapid grower mycobacterium. | the rapid grower mycobacteria have emerged as significant group of human pathogen amongst the runyon group iv organisms that are capable of causing infection in both the healthy and immunocompromised hosts. study aimed to identification of species amongst rapid grower non tuberculous mycobacterial isolates by polymerase chain reaction - restriction enzyme analysis (pra). analysis and comparison of results with standard biochemical tests. | 2018 | 29332650 |
| antibiotic resistance in mycobacterium abscessus and mycobacterium fortuitum isolates from malaysian patients. | rapidly growing mycobacterial species (rgm) are increasingly being recognized as the cause of various superficial and deep infections in humans. two of the species most frequently isolated from clinical specimens are mycobacterium abscessus and mycobacterium fortuitum. both species are associated with antibiotic resistances that may complicate therapy. this paper describes the pattern of resistance to five antibiotics commonly prescribed for rgm infections, in m. abscessus and m. fortuitum isola ... | 2017 | 29171453 |
| antibiofilm activity of nanoemulsions of cymbopogon flexuosus against rapidly growing mycobacteria. | rapidly growing mycobacteria (rgm) are opportunistic microorganisms that can cause both local and disseminated infections. when in biofilm, these pathogens become highly resistant to antimicrobials used in clinical practice. composed abundantly of polymeric substances, biofilms delay the diffusion of antimicrobials, preventing the drug from penetrating the deeper layers and having an effective action. therefore, the search for new and alternative therapeutic options has become of fundamental imp ... | 2017 | 29122674 |
| molecular mechanisms of intrinsic streptomycin resistance in mycobacterium abscessus. | streptomycin, the first drug used for the treatment of tuberculosis, shows limited activity against the highly resistant pathogen mycobacterium abscessus we recently identified two aminoglycoside-acetylating genes [aac(2') and eis2] which, however, do not affect susceptibility to streptomycin. this suggests the existence of a discrete mechanism of streptomycin resistance. m. abscessus blastp analysis identified mab_2385 as a close homologue of the 3″-o-phosphotransferase [aph(3″)] from the oppor ... | 2018 | 29061744 |
| characterization of non-tuberculous mycobacterium from humans and water in an agropastoral area in zambia. | the non-tuberculous mycobacteria include those mycobacterium species that are not members of the mycobacterium tuberculosis complex, the causative agent of pulmonary tuberculosis and mycobacterium leprae. in zambia, non-tuberculous mycobacteria are gaining recognition as pathogens of public health significance. however, there is scanty information on the isolation and speciation of these organisms for better patient management, consequently reducing the burden of these infections. given the abov ... | 2018 | 29310592 |
| lipase-catalyzed kinetic resolution of novel antitubercular benzoxazole derivatives. | novel benzoxazole derivatives were synthesized, and their antitubercular activity against sensitive and drug-resistant mycobacterium tuberculosis strains (m. tuberculosis h37 rv, m. tuberculosis sp. 210, m. tuberculosis sp. 192, mycobacterium scrofulaceum, mycobacterium intracellulare, mycobacterium fortuitum, mycobacterium avium, and mycobacterium kansasii) was evaluated. the chemical step included preparation of ketones, alcohols, and esters bearing benzoxazole moiety. all racemic mixtures of ... | 2017 | 29274237 |
| non-tuberculosis mycobacterium speciation using hplc under revised national tb control programme (rntcp) in india. | non-tuberculous mycobacteria (ntm) are ubiquitous in nature. the data on prevalence of ntm under the rntcp is scarce. many ntm species have clinical significance, and hence their identification and speciation are important. | 2018 | 28990320 |
| combinatorial design and virtual screening of potent anti-tubercular fluoroquinolone and isothiazoloquinolone compounds utilizing qsar and pharmacophore modelling. | the virulence of tuberculosis infections resistant to conventional combination drug regimens cries for the design of potent fluoroquinolone compounds to be used as second line antimycobacterial chemotherapeutics. one of the most effective in silico methods is combinatorial design and high throughput screening by a ligand-based pharmacophore prior to experiment. the combinatorial design of a series of 3850 fluoroquinolone and isothiazoloquinolone compounds was then screened virtually by applying ... | 2018 | 29347843 |
| natural history of mycobacterium fortuitum pulmonary infection presenting with migratory infiltrates: a case report with microbiological analysis. | presence of mycobacterium fortuitum in respiratory tracts usually indicates mere colonization or transient infection, whereas true pulmonary infection occurs in patients with gastroesophageal disease. however, little is known about the diagnostic indications for true m. fortuitum pulmonary infection and the natural history of the disease. | 2018 | 29291713 |
| mycobacterium fortuitum infection at umbilical hernioplasty site. | non tuberculous mycobacteria (ntm) are a group of rapidly growing mycobacteria and are generally considered to be of low virulence. of late, there has been an increase in incidence of infections due to these organisms. among them, mycobacterium fortuitum, m. chelonae and m. abscessus are the common species which have been identified. though they are occasionally implicated in pulmonary infections, ntm are very commonly associated with cutaneous infections, especially surgical site infections. id ... | 2017 | 29207758 |
| high rates of nontuberculous mycobacteria isolation from patients with presumptive tuberculosis in iran. | nontuberculous mycobacteria (ntm) can cause disease which can be indistinguishable from tuberculosis (tb), posing a diagnostic and therapeutic challenge, particularly in low- and middle-income settings. we aimed to investigate the mycobacterial agents associated with presumptive clinical pulmonary tb in iran. a total of 410 mycobacterial isolates, obtained between march 2014 and january 2016, from 7600 clinical samples taken from consecutive cases of presumptive diagnosis of tb were identified. ... | 2018 | 29188063 |
| [co-infection by mycobacterium fortuitum and mycobacterium tuberculosis in splenic abscesses in a patient with hiv]. | patients with hiv are susceptible to mycobacterium infection. in the case of fast-growing mycobacteria, the group to which mycobacterium fortuitum (m. fortuitum) belongs, infections have been described in the skin, lungs, lymph nodes and disseminated disease. we present the case of a 43-year-old male patient with pre-diagnosis of hiv in antiretroviral therapy, which comes as a fever, asthenia, weight loss and chronic diarrhea. abdominal tomography is performed and hepatosplenomegaly is evidenced ... | 2017 | 29177396 |
| recurrent mycobacterium fortuitum insulin injection site abscesses. | | 2017 | 29168906 |
| frequency of mycobacterium bovis and mycobacteria in primary immunodeficiencies. | susceptibility to mycobacterial diseases is observed in some primary immunodeficiency diseases. in this study, we aimed to evaluate mycobacterial infections in primary immunodeficiency diseases. | 2017 | 29062247 |
| biological evaluation of diphenyleneiodonium chloride (dpic) as a potential drug candidate for treatment of non-tuberculous mycobacterial infections. | novel drug discovery against non-tuberculous mycobacteria is beset with a large number of challenges including the existence of myriad innate drug resistance mechanisms as well as a lack of suitable animal models, which hinders effective translation. in order to identify molecules acting via novel mechanisms of action, we screened the library of pharmacologically active compounds against non-tuberculous mycobacteria to identify such compounds. | 2017 | 28961864 |
| implantable cardiac defibrillator infections: the emerging importance of mycobacterium fortuitum. | infection is a clinically relevant complication associated with intracardiac devices. atypical mycobacteria, particularly mycobacterium fortuitum, have been increasingly implicated in cardiovascular implantable electronic device (cied) infections. we present a case of m. fortuitum cied infection in a patient with ischaemic cardiomyopathy occurring approximately 3 weeks after insertion. the recognition and adequate treatment, including device removal, tissue sampling and the determination of anti ... | 2017 | 28928258 |
| articular sinus tract with mycobacterium fortuitum osteomyelitis after anterior cruciate ligament reconstruction: a case report. | three weeks after transphyseal anterior cruciate ligament reconstruction with a hamstring autograft, a fourteen-year-old boy presented with fevers, joint effusion, and elevated inflammatory markers. cultures from an operative debridement grew mycobacterium fortuitum, for which he received six months of antibiotics. continued drainage prompted magnetic resonance imaging, which revealed osteomyelitis of the tibial tunnel. | 2017 | 29252811 |