| cholera old and new. | | 1977 | 327479 |
| a bactericidal effect for human lactoferrin. | streptococcus mutans and vibrio cholerae, but not escherichia coli, were killed by incubation with purified human apolactoferrin. concentrations of lactoferrin below that necessary for total inhibition resulted in a marked reduction in viable colony-forming units. this bactericidal effect was contingent upon the metal-chelating properties of the lactoferrin molecule. | 1977 | 327545 |
| recent developments in diarrheal diseases. | diarrheal diseases result from two different processes: toxin elaboration by pathogens such as vibrio cholerae and some strains of escherichia coli and invasion of tissue, eg, by shigellae and salmonellae. intestinal motility serves as a normal cleansing mechanism of the intestine, and drugs that decrease this motility may facilitate replication of pathogens and their attachment to or invasion of the intestinal tissue. therapy should not be aimed at suppressing the symptom of diarrhea. it is now ... | 1977 | 329247 |
| [role of antibiotics in the ecology of cholera vibrios]. | | 1977 | 329747 |
| [disinfecting effect of fixatives in histoenzymatic analysis of vibrio cholerae]. | data are presented on the disinfecting action of fixative fluids on the cholera causative agents in the freshly frozen sections from the tissues and organs of animals infected with cholera. the results obtained offered a possibility of drawing a conclusion on the harmlessness of the histoenzymatic studies on the material infected with cholera vibrio. | 1977 | 331762 |
| [trial of the toxins of the sonne microbe and cholera vibrio in an isolated loop of mouse small intestine]. | enterotoxic properties of neuro-and endotoxins of sonne dysentery bacillus, of cholerogen, and bowen's cholera vibrio endotoxin were determined on a model of an isolated loop of the mouse small intestine. in definite doses all the mentioned preparations could induce dilatation of the isolated loop of the mouse small intestine. in case of sonne bacillus neurotoxin this property correlated with the toxicity of the preparation for the animals and failed to depend on the virulence and biochemical re ... | 1977 | 331786 |
| the problems of oral immunisation. | | 1977 | 332136 |
| identification and treatment with a long-acting sulphonamide of 15 carriers of vibrio cholerae el tor serotype ogawa, during the 1973 cholera epidemic in naples. | during the cholera epidemic in 1973, nine contact carriers of vibrio cholerae el tor serotype ogawa were identified in a family from naples as well as six others coming from different parts of the city or its surroundings. all the subjects were admitted to the quarantine ward of the 'cotugno hospital' (naples) in which they remained continuously for 14 or 16 days. during this time these bacteriologically confirmed carriers were treated orally with a long-acting sulphonamide (sulphamethoxypyrazin ... | 1977 | 332466 |
| microtiter solid-phase radioimmunoassay for detection of escherichia coli heat-labile enterotoxin. | the development of a microtiter solid-phase radioimmunoassay for the detection of vibrio cholerae enterotoxin and heat-labile escherichia coli enterotoxin is described. the test is based on the immunological similarity between v. cholerae toxin and e. coli heat-labile toxin. the assay is easy to perform, quantitative, and at least as sensitive and specific as the y-1 adrenal cell system. | 1977 | 332626 |
| algorithms in the diagnosis and management of exotic diseases. xxvi. cholera. | | 1977 | 333039 |
| bismuth subsalicylate inhibits activity of crude toxins of escherichia coli and vibrio cholerae. | the inhibition of the activity of enterotoxins by bismuth salts was assessed in laboratory models. in the y-1 adrenal cell tissue culture system, a commercial preparation containing bismuth subsalicylate reduced the activity of crude toxin from vibrio cholerae by 10(4)-fold as compared with the activity of controls. similar results were obtained with use of the adult rabbit ligated intestinal loop model. the preparation failed to affect crude escherichia coli or cholera toxin activity once these ... | 1977 | 335003 |
| systemic effect of vibrio cholerae and escherichia coli enterotoxins on plasma glucose and alkaline phosphatase. | | 1977 | 336525 |
| enzyme-linked immunosorbent assay for detection of escherichia coli heat-labile enterotoxin. | the development of an enzyme-linked immunosorbent assay (elisa) for the detection of heat-labile escherichia coli enterotoxin is described. the assay, which is based on the immunological similarity between vibrio cholerae toxin and heat-labile e. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. the elisa system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat ... | 1977 | 336638 |
| cross-neutralization reactions of escherichia coli and vibrio cholerae enterotoxins as studied by rabbit skin inoculation test. | neutralization of enterotoxins of vibrio cholerae 569 b and escherichia coli 10407 by antitoxins to v. cholerae 569 b, e. coli 334, 408-3 and 10407 was studies by intradermal inoculation test in the rabbit. neutralization of v. cholerae enterotoxin by homologous as well as heterologous antisera of e. coli was observed, except that there was no neutralization of the enterotoxin by antiserum to e. coli 408-3 enterotoxin. neutralization of e. coli enterotoxin to a varied extent by homologous as wel ... | 1977 | 337760 |
| [new methods of laboratory diagnosis of plaque and cholera]. | | 1977 | 339614 |
| enterotoxin antibodies in relation to diarrhoea in swedish soldiers in cyprus. | the development of antibodies to escherichia coli heat-labile enterotoxin (lt) and its relationship to diarrhoea was studied in two swedish battalions (one in the winter, wb, and one in the summer, sb) of the united nations peace-keeping force in cyprus. whereas only 6-15% of the soldiers had detectable levels of anti-lt antibodies in their sera before leaving sweden, as many as 69% of the sb and 43% of the wb soldiers who developed diarrhoea in cyprus acquired significant titres of enterotoxin ... | 1977 | 340076 |
| the agglutination reactions of cholera vibrios. | the present study with 11 strains of vibrio using single-dose and hyperimmune antisera confirmed earlier observations on the cross-reactivity of the flagellar (h) agglutinating antigens of cholera and nag vibrios. the effect of several variables on the agglutinating sensitivity of cell suspensions was determined by measuring the reaction rate in the presence of constant o- or h-antibody. the variables investigated were culture conditions, antigen dilution, reaction temperature, formalin fixation ... | 1977 | 340734 |
| cholera: the relevance of the irrelevant. | | 1977 | 341599 |
| stimulatory effect of vibrio cholerae neuraminidase on the antibody response to various antigens. | | 1978 | 342397 |
| studies on immunologic cross-reactions of enterotoxins from escherichia coli and vibrio cholerae. | | 1977 | 342403 |
| immunological properties of vibrio cholerae lipopolysaccharides. | immunological effects of wall lipopolysaccharide (lps) preparations obtained from vibrio cholerae inaba 569b, ogawa nih 41 and nag 4715 strains by the hot phenol-water procedure were examined in mice. although these lps lack kdo, which are basic components of the core region of most gram-negative lps, they still have potencies as b-cell mitogens, adjuvants, immunosuppressants, polyclonal b-cell activators and phagocytic stimulants for macrophages. the activities of these v. cholerae lps on murin ... | 1977 | 342874 |
| [vibrion variability under the action of antibiotics]. | tetracycline, streptomycin and monomycin resistant variants of the cholera and nag-vibrios were obtained by means of repeated passages on nutrient media with increasing concentrations of the antibiotics (114 variants v. cholerae asiaticae, 1337 variants of v. cholerae eltor and 299 variants of nag-vibrios of the 1st heiberg group). the highest number of the antibiotic resistant variants was obtained under the effect of streptomycin and the resistance level to it was much higher (up to 8000 gamma ... | 1978 | 343710 |
| acid precipitation of clostridium botulinum type c and d toxins from whole culture by addition of ribonucleic acid as a precipitation aid. | the ratios of ribonucleic acid to protein contents of clostridium botulinum type c, d, and e cultures were lower than those of type a, b, and f cultures. addition of ribonucleic acid at 0.4 mg/ml to culture satisfactorily aided acid precipitation of type c and d toxins, but not that of type e toxin. | 1978 | 344224 |
| eosinophil chemotactic factor (ecf). iii. generation in human peripheral leukocytes. | eosinophil chemotactic factor (ecf) can be released from human peripheral leukocytes by an ige-anti-ige reaction, by the calcium ionophore a 23187 and during phagocytosis. supernatants and sonicates of unstimulated cells contain little or no ecf. on stimulation, however, ecf activity increases in the cells and even more so in the supernatants. this holds for purified neutrophils (pmns) as well as for basophil-containing mononuclear cell preparations. these findings contrast with those in lung ho ... | 1978 | 344230 |
| acute gastro-enteritis (a clinical and bacteriological study). | | 1977 | 344805 |
| affinity filters, a new approach to the isolation of tox mutants of vibrio cholerae. | we have devised a novel plate assay method for detecting mutants of vibrio cholerae altered in the production of cholera toxin (tox mutants). colonies replicated from a master plate are grown on the surface of a cellulose filter disc to which ganglioside-albumin conjugates have been attached. toxin secreted by the colonies is tightly bound to the ganglioside filters. after removal of the cells by washing, the bound toxin may be detected by treating the filters with radioactively labeled antibodi ... | 1978 | 345281 |
| antibodies in human serum and milk induced by enterobacteria and food proteins. | ingestion of escherichia coli o83 bacteria by adults resulted in a transient irregular colonization leading to a serum antibody response in only four out of 14 cases examined. in all of three pregnant women, however, iga antibodies against e. coli o83 antigen were released from colostral cells after similar bacterial ingestion although no serum antibody response was noted. the findings indicate a link between the antigenic exposure to the gut and secretory antibodies of the iga class, presumably ... | 1977 | 346319 |
| [complications following sclerosing therapy of hemorrhoids]. | | 1978 | 346331 |
| effect of vibrio cholerae neuraminidase on the phagocytosis of e. coli by macrophages in vivo and in vitro. | the mechanisms by which vibrio cholerae neuraminidase vcn acts as an adjuvant is unclear. we have studied the effect of vcn on the phagocyotsis of e. coli 0111 k58 by macrophages in vivo and in vitro. (1) using and in vivo intraperitoneal clearance test we have shown that prior injection of vcn (50 units/mouse) into the peritoneal cavity of mice enhances the clearance of the bacteria as efficiently as an optimal amount of opsonizing antiserum. we have also shown that the enhancement of intraperi ... | 1978 | 346477 |
| ["the relationship between bacteriology and epidemiology" - and what became of it]. | | 1978 | 347260 |
| the mechanism of depressed cardiac output on positive end-expiratory pressure (peep). | | 1978 | 347615 |
| [autoflora of the skin as an indicator of the body's immunobiological reactivity]. | | 1978 | 347690 |
| pharmacological and biochemical evidence for the dopamine agonistic effect of bromocriptine. | | 1978 | 347864 |
| prevention of implantation by [d-trp6-lh-rh in the rat: comparative study with the effects of large doses of hcg on pregnancy. | effect of large doses of [d-trp6]-lh-rh and hcg on implantation of fertilized ova was examined in the rat. subcutaneous administration of 6ug[d-trp6]-lh-rh per day from days 1 to 5 of pregnancy by an implanted minipump completely prevented the implantation of fertilized ova associated with a dramatic reduction of plasma progesterone on days 4 and 8, with a slight reduction of estradiol on day 4. there was no balooning of the uterus in the exploratory laparatomy on days 8 and 14. administration o ... | 1977 | 350578 |
| pattern of haemagglutination and haemolysis tests in v. cholerae biotype el tor recently isolated from howarah hospital. | | 1977 | 350772 |
| ribitol and d-arabitol catabolism in escherichia coli. | in escherichia coli c, the catabolism of the pentitols ribitol and d-arabitol proceeds through separate, inducible operons, each consisting of a dehydrogenase and a kinase. the ribitol operon is induced in response to ribulose, and the d-arabitol operon is induced in response to d-arabitol. each operon is under negative control. the genes of the ribitol and d-arabitol operons are very closely linked and lie in a mirror image arrangement, rtlb-rtla-rtlc-atlc-atla-atlb, between metg and his on the ... | 1978 | 350825 |
| selective inhibition of klebsiella aerogenes growth on pentoses by pentitols. | selective inhibition of growth by pentitols was observed when klebsiella aerogenes m-7 which could not utilize pentitols was grown on pentoses. d-arabitol inhibited the growth on d-arabinose as a sole carbon source, but had no effect on the growth on l-arabinose, d-xylose, and d-ribose. similarly, l-arabitol inhibited the growth on d-arabinose and l-arabinose, ribitol inhibited the growth on d-arabinose and l-arabinose, and xylitol inhibited the growth on d-xylose. from the following reasons, we ... | 1978 | 350845 |
| analogs of the dnab gene of escherichia coli k-12 associated with conjugative r plasmids. | the dnab266(am) mutation in escherichia coli k-12 is an amber mutation such that strains carrying this mutation are not viable in a sup+ strain. with five different r plasmids, it has been possible to construct viable r+ derivatives of this amber mutant and show that the plasmids themselves do not carry amber suppressors. this is interpreted as evidence for the presence of dnab analog genes associated with these plasmids. plasmid-positive strains carrying these genes often showed some degree of ... | 1978 | 350846 |
| electron microscope heteroduplex studies of sequence relations among plasmids of escherichia coli: structure of f100, f152, and f8 and mapping of the escherichia coli chromosomal region fep-supe-gal-attlambda-uvrb. | the genetic and physical structures of commonly used f-prime factors carrying the galactose region of the escherichia coli chromosome were analyzed. deletions in the chromosomal dna sequences in the f-prime factors were found to be frequent events. a genetic method was developed to reconstruct the original f-prime factors from deletion variants. heteroduplex analysis of the reconstructed f-prime factors confirmed the derivation of the f-prime factors f100 and f152, from the same hfr, and finally ... | 1978 | 350847 |
| rich culture medium for the radiochemical labeling of proteins and nucleic acids. | yeast extract was treated with tyrosine decarboxylase and used to prepare a rich, complex medium virtually free of tyrosine. the medium supported maximal growth rates for escherichia coli prototrophs, as well as for defined and undefined auxotrophs. it has made possible the efficient radiochemical labeling of cells growing optimally in complex medium and the characterization of mutants with undefined requirements. similarly prepared media may be useful for the study of fastidious organisms and o ... | 1978 | 350856 |
| n10-formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in escherichia coli. | glycinamide ribotide transformylase from escherichia coli was obtained free of n5,n10-methenyltetrahydrofolate cyclohydrolase activity by deae-cellulose chromatography. in reaction mixtures containing this enzyme preparation in potassium maleate buffer, ph 7.2, no detectable interconversion of n5,n10-methenyltetrahydrofolate occurred. upon addition of glycinamide ribotide, n-formylglycinamide ribotide was formed when n10-formyltetrahydrofolate was present; no formylation occurred in the presence ... | 1978 | 350869 |
| assay method for vibrio cholerae and escherichia coli enterotoxins by automated counting of floating chinese hamster ovary cells in culture medium. | as chinese hamster ovary (cho) cells on plastic proliferate, many cells float off into the medium instead of piling up after they form a monolayer. fewer cells were floating in the medium when cho cells were incubated with cholera toxin at a concentration as low as 10 pg/ml. the toxin increased the adhesiveness of the cells forming confluent monolayers so that the floating cells accumulated on the adherent monolayers. on the basis of this finding, a simple, quantitative assay method for cholera ... | 1978 | 350897 |
| influenza as a problem in immunology. | | 1978 | 351051 |
| sequential expression of immunoglobulin on developing mouse b lymphocytes: a systematic survey that suggests a model for the generation of immunoglobulin isotype diversity. | paired immunofluorescent staining with antibodies specific for the major isotypes of mouse immunoglobulin was used to study the ontogenetic expression of diversity of cell surface immunoglobulin. the first b lymphocytes to emerge, derived from cytoplasmic igm+ precursors, express sigm exclusively. between birth and 3 days of age separate populations of sigm+ b lymphocyte acquire a second isotype: sigd, one of the subclasses of sigg, or siga. at 3 days, all splenic b lymphocytes that bear sig or ... | 1978 | 351064 |
| [affinity modification of escherichia coli ribosomes in the region of the mrna-binding center by a heptauridylate analog bearing a chemically active group at the 3' end]. | 4-(n-2-chloroethyl-n-methylamino)-benzaldehyde acetyl derivative (rcl-derivative) of hepatauridylic acid was used to localize the structure organizing the mrna-binding site of ribosomes. this derivative; like a free oligonucleotide, stimulates the binding of [14c]phenylalanyl-trna to ribosomes and effectively alkylates ribosomes, mainly the 30s subunit within the specific complex. the alkylation being completely inhibited by preincubation with polyuridylic acid, suggests that the chemical altera ... | 1978 | 351372 |
| a bacteriological study of some frozen and nonfrozen foods. | over a period of 19 months, a total of 331 food samples were submitted to the food section of the bacteriology division for bacteriological examination. these included 184 samples of frozen seafoods from exporters and 147 samples of fresh, nonfrozen foods from food caterers. the total bacterial count for frozen seafoods ranged from 1 x 10(2) to 2.98 x 10(6) per gm with a mean of 2.14 x 10(5) per gm. coliforms, escherichia coli and staphylococcus aureus were present in 48.9%, 3.3% and 8.2% of the ... | 1977 | 351818 |
| [role of normal microflora in the treatment of vibrio carrier state in rat gnotobionts]. | materials on the study of interactions between vibrio cholera el tor and some indigenous bacteria of human intestinal tract, which proved to be active antagonists in vitro, are presented in this work. experiments were conducted on gnotobiotic rats (fisher), using various combinations and variants of the order of the microorganism administration; there were revealed no antagonistic relations between virbrio cholerae el tor and escherichia coli m-17, lactobacillus fermenti, lactobacillus plantarum ... | 1978 | 352437 |
| [suture of vessels in microsurgery (author's transl)]. | | 1978 | 352482 |
| preparation of acid-fast microscopy smears for proficiency testing and quality control. | a method is presented for preparing smears for proficiency testing and quality control in acid-fast microscopy. the work was prompted by the increased demand for acid-fast bacilli positive smears with characteristic microscopic appearance and among-smear uniformity. | 1978 | 353070 |
| monoamine-containing paraneurons in the sympathetic ganglia of mammals. | 1. a combined method for glyoxylic acid induced catecholamine fluorescence and electron microscopy was introduced in this study of the small intensely fluorescent (sif) or granule-containing (gc) cells of the sympathetic ganglia. 2. adrenergic interneurons were demonstrated in the sympathetic ganglia of the rat and the rhesus monkey. 3. existence of adrenergic paraneurons (neuro-endocrine cells) in the sympathetic ganglia of several mammalian species was suggesed by the following observations: a ... | 1977 | 354578 |
| lymphoblast transformation in rats convalescent from infection with plasmodium berghei. | | 1978 | 355194 |
| a clinical and bacteriological study of acute diarrhoea in adults. | | 1978 | 355830 |
| [analysis of the thermostability of the hybrid dna molecules of microorganisms as a means of increasing the resolution of the molecular hybridization technic]. | a comparative study of termostability of microorganisms dna was performed in order to increase the resolution of the method of molecular hybridization. molecular hybridization was carried out and the curve of hybrid dna duplexes distribution, acccording to termostability of two groups of microorganisms, related to strains echerichia coli b1 and vibrio cholerae eltor 18647, were obtained. it was determined that the form of the curves is specie specific for the microorganisms investigated but ther ... | 1978 | 355865 |
| cholera and related diarrhoeas ('turista'). | | 1978 | 357986 |
| radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of escherichia coli or vibrio cholerae. | a rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of escherichia coli or vibrio cholerae growing on media solidified with agar. the applicability of this method for isolating tox mutants of e. coli and v. cholerae has been demonstrated. | 1978 | 359590 |
| reovirus-like agent and enterotoxigenic escherichia coli infections in pediatric diarrhea in the philippines. | of 82 children hospitalized with diarrhea in the philippines during january-june 1976, 14 (17%) had infections due to a reovirus-like agent as determined by detection of viral particles in stools by electron microscopy (12 [15%] of 82) and/or by a rise in titer of antibody to the serologically related nebraska calf diarrhea virus (eight [20%] of 39). escherichia coli producing heat-labile enterotoxin were found in six (7%) of 82 ill children and two (4%) of 49 healthy control children, while e. ... | 1978 | 359725 |
| enterotoxicity of the so-called nag vibrios. | | 1978 | 361004 |
| immunological cross-reactivity between a heat-labile enterotoxin(s) of escherichia coli and subunits of vibrio cholerae enterotoxin. | heat-labile enterotoxin from escherichia coli is immunologically related to both subunits, a and b, of cholera enterotoxin as demonstrated by neutralization and immunodiffusion. | 1978 | 361573 |
| effects of injected iron and siderophores on infections in normal and immune mice. | the fate of virulent and avirulent strains of salmonella typhimurium in untreated and iron-injected mice and in transferrin-containing media demonstrated a direct relationship between bacterial virulence and the ability of bacteria to acquire transferrin-bound iron. effects of injected iron on the development of infections with virulent and avirulent bacterial strains were determined in normal and immune mice by determinations of bacterial numbers in tissue homogenates and the mortality of infec ... | 1978 | 365761 |
| ecology, serology, and enterotoxin production of vibrio cholerae in chesapeake bay. | a total of 65 isolates of vibrio cholerae, serotypes other than o--1, have been recovered from water, sediment, and shellfish samples from the chesapeake bay. isolations were not random, but followed a distinct pattern in which salinity appeared to be a controlling factor in v. cholerae distribution. water salinity at stations yielding v. cholerae (13 out of 21 stations) was 4 to 17 0/00, whereas the salinity of water at stations from which v. cholerae organisms were not isolated was less than 4 ... | 1979 | 367273 |
| secretory iga antibodies to enterobacterial virulence antigens: their induction and possible relevance. | 1) milk and salivary s-iga antibodies are via the homing of iga producing cells from the peyer's patches closely connected with antigenic stimuli in the intestine. this explains the presence in human milk of s-iga antibodies against e. coli o and k antigens, v. cholerae and shigella o antigens, e. coli and v. cholerae enterotoxins. these secretory antibodies can be induced by intestinal exposure and boosted by parenteral vaccination. 2) preliminary data suggest that the iga response in the urina ... | 1978 | 369311 |
| adhesion and chemotaxis as determinants of bacterial association with mucosal surfaces. | 1) chemotaxis of cholera vibrios facilitates the association of these bacteria with the mucosal surface. 2) mucosal extracts can block the chemotactic receptors on the bacterial surface and thereby retard the association of chemotactic bacteria with the mucosal surface. 3) chemotactic cholera vibrios grow more efficiently than non-chemotactic mutants in germfree mice and in isolated intestinal loops of rabbits. conversely, non-chemotactic mutants grow more efficiently in infant mice. 4) chemotax ... | 1978 | 369312 |
| [the role of interhuman contacts in the transmission of cholera, epidemics of 1832 and 1854]. | by mapping the evidence of excess mortality month by month for cholera epidemics of 1832 and 1854, which were the most deadly and most typical of their kind in france, the authors are able to provide a kinetic description of the propagation of the asian disease throughout the whole of france. traditionally it has been assumed that hydric anademia explains, for the most part, the paths taken by the disease and its varying intensity, but the importance of direct interhuman contamination is demonst ... | 1978 | 369727 |
| immunological studies on the ammonium sulphate fractions of vibrio cholerae cell lysate. | | 1978 | 369995 |
| [reactogenicity and immunological effectiveness of an oral cholera chemical vaccine in a limited controlled experiment with human revaccination]. | oral cholera chemical vaccine in the doses tested (2 and 3 tablets) proved to be areactogenic, harmless and immunologically effective in a controlled limited trial in 150 volunteers. by the results of titration of specific antitoxins and vibriocidal antibodies in the blood serum, as well as of coproantibodies a dose of 2 tablets was chosen as the optimal one. | 1978 | 371293 |
| adaptation of the staphylococcal coagglutination technique for detection of heat-labile enterotoxin of escherichia coli. | protein a-containing staphylococci coated with specific antiserum were tested for heat-labile enterotoxin of escherichia coli. the immunological cross-reactivity of e. coli heat-labile enterotoxin with vibrio cholerae toxin (choleragen) was the basis for sensitizing stabilized suspensions of the cowan i strain of staphylococcus aureus with anticholeragen. unconcentrated culture supernatant fluid containing e. coli heat-labile enterotoxin produced macroscopic agglutination when mixed with sensiti ... | 1979 | 372214 |
| the minimum physiological mobility requirement for man supported on a soft surface. | | 1979 | 372891 |
| [bcg sepsis as a fatal complication of bcg vaccination]. | fatal septicemia with bacillus calmette-guérin (bcg) following bcg vaccination is always caused by an underlying defect of immunologic mechanisms. at the children's hospital, university of berne, two cases of bcg septicemia were recently observed. the clinical picture, course, and pathogenesis of the illness are discussed. the harmless complications of bcg vaccination are summarized, and a review of the literature on fatal disseminated bcg infection stresses the different type of immunologic mec ... | 1979 | 373107 |
| [universal mechanism for regulating blood clotting and fibrinolysis]. | | 1979 | 373283 |
| [comparative evaluation of different globulin preparations and of the means for blood sensitization in obtaining antibody erythrocytic diagnostica]. | in comparing the hemosensitizing activity of various immunoglobulin preparations with different methods of erythrocyte loading the greatest activity and specificity was revealed in igg isolated by means of deae-sephadex a-50. the method of erythrocyte sensitization with the use of alizarin blue indicator was more advantageous in respect to sensitivity and specificity of sh. sonnei and newcastle indication over boyden's, jandl and simmons', bing's methods. | 1979 | 373342 |
| [typing of escherichia coli o and k antigens in patients with urinary tract infections (author's transl)]. | in a long-term investigation in children and adult patients with urinary tract infection, 1403 escherichia coli strains were serotyped and 266 strains were subjected to serological investigation. the most frequently occurring o groups were o6, o8, o2, o18, o4, o75, o1, o22, o7 and o9. l type antigens were predominant among the k antigens. infants in whom pyelonephritis manifested itself for the first time exhibited an above average rate of infection with o2, o4 and o75. a reinfection rate of 84 ... | 1979 | 374279 |
| protein and ribonucleic acid syntheses in heat-damaged and heat-killed escherichia coli. | protein and ribonucleic acid (rna) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. immediately after heating, cells were incubated in growth media containing either [14c]leucine or [3h]uracil. the labeled cells were subsequently treated with penicillin. viable cells were shown to lyse, and the intact nonviable cells were collected by centrifugation. the results showed that after heating, protein and rna synthesis were rein ... | 1979 | 374390 |
| light effects in yeast: evidence for participation of cytochromes in photoinhibition of growth and transport in saccharomyces cerevisiae cultured at low temperatures. | visible light of moderate intensity inhibits growth, respiration, protein synthesis, and membrane transport in bakers' yeast and has a deleterious effect on membrane integrity. the results of this study indicate that these effects require the presence of cytochromes b and a/a(3). the light sensitivities of growth rate and [(14)c]histidine uptake in wild-type rho(+) y185 and d225-5a strains of saccharomyces cerevisiae were compared with those in a variety of mutants lacking cytochrome b or a/a(3) ... | 1979 | 374392 |
| enteric gram-negative rods, 1976. | | 1979 | 375729 |
| [use of india ink for the purpose of the specific detection of pathogenic microorganisms in histological preparations]. | | 1979 | 377872 |
| infectious mononucleosis associated with fatal beta hemolytic streptococcal infection. | | 1979 | 378507 |
| subcutaneous corynebacterium parvum in bladder cancer: a controlled study of its immunological effects. | fourteen out of 26 patients with invasive bladder cancer were randomly assigned to receive weekly subcutaneous injections of corynebacterium parvum (cp) in addition to standard treatment. peripheral blood t lymphocyte percentage, k cell activity, mitogen responsiveness, and monocyte and polymorph leucotaxis were measured at intervals over a period of 1 to 2 years. the only consistent difference between the cp-treatment patients and the controls was a slightly higher level of k cell activity in t ... | 1979 | 380732 |
| delineation of igm-receptor bearing human t and b lymphocytes using a direct plaque forming cell (pfc) assay. | based on the observation that binding of igm cytophilic antibodies to lymphocytes is temperature dependent, a direct plaque forming cell (pfc) assay was developed to detect igm-receptor bearing human peripheral blood t and b lymphocytes. lymphocytes were passively sensitized with igm anti-srbc molecules at 4 degrees c, added to srbc monolayers then incubated at 37 degrees c with guinea pig complement to develop the plaques. the pfc assay has methodological advantages over rosetting methods which ... | 1979 | 381530 |
| demonstration of igm receptors on human peripheral blood monocytes using a direct plaque forming cell (pfc) assay. | | 1979 | 381531 |
| [vibrio cholerae toxin and "enterotoxinogenic" colibacillus toxins. detection]. | | 1979 | 381796 |
| antibiotics produced by streptomyces olivaceus 142. antifungal properties of antibiotic wr-142 fpg. | antifungal activity of antibiotic wr-142 fpg was evaluated in vitro and in vivo. in vitro, the minimal inhibitory concentration (mic) for candida and rhodotorula was 0.1 microgram/ml (preparation i) and 0.003 microgram/ml (preparation ii). in vivo, experiments were carried out with model of subacute candidiasis in balb mice. the criteria for antifungal activity of wr-142 fpg were based on survival time of infected animals and numbers of live c. albicans cells in the kidneys and spleen of animals ... | 1979 | 383040 |
| studies of yeast phenylalanine-accepting transfer ribonucleic acid backbone structure in solution by phosphorus-31 nuclear magnetic resonance spectroscopy. | approximately 17 diester phosphates from the backbone structure of yeast trnaphe give rise to phosphorus resonances, which are resolved in its 31p nmr spectrum. to localize these diester phosphates within the trna structure, 31p nmr spectra of several chemically or enzymatically modified yeast trnaphe species were recorded. to this end selective modifications were performed in the anticodon, the dhu, and the t psi c loop. modifications, performed in different loop regions, give rise to perturbat ... | 1979 | 383144 |
| age-dependent increase in the formation of prostaglandin i2 by intact and homogenised aortae from the developing spontaneously hypertensive rat. | the prostaglandin i2 biosynthetic capacity of aortae from spontaneously hypertensive rats of various ages (1, 2, 3, 4, and 5 months) was investigated. an age-dependent increase in enzyme activity was observed reaching maximum by three months of age which correlated well with the age- related increase in the systolic blood pressure. these results support our notion that the enhanced aortic synthesis of the potent vasodepressor prostaglandin i2 by the spontaneously hypertensive rat likely represen ... | 1979 | 383156 |
| [investigation on the presence of salmonella in drinking water from water supplies and distribution systems in togo (author's transl)]. | bacteriological drinking water examination of samples from water works and wells as well as from their distribution systems revealed that from 26 samples, where salmonella organisms could be isolated, in 16 cases e. coli- and coliforms could not be found and that in 9 of these cases even the colony count was below 100 colonies per ml at 20 degrees c and 37 degrees c incubation temperature. the common reasons for such contaminations are technical deficiencies, mostly faulty well-heads or broken c ... | 1979 | 384726 |
| importance of medium in demonstrating penicillin tolerance by group b streptococci. | a total of 30 clinical isolates of group b streptococci were studied for penicillin tolerance in vitro. minimal inhibitory and bactericidal concentrations of penicillin were determined simultaneously in three test media which have been used for group b streptococci, tryptose phosphate, mueller-hinton, and todd-hewitt broths, using a logarithmic-phase inoculum of 10(5) colony-forming units per ml. minimal inhibitory concentrations in the three media did not differ significantly. however, minimal ... | 1979 | 384895 |
| importance of medium in demonstrating penicillin tolerance by group b streptococci. | a total of 30 clinical isolates of group b streptococci were studied for penicillin tolerance in vitro. minimal inhibitory and bactericidal concentrations of penicillin were determined simultaneously in three test media which have been used for group b streptococci, tryptose phosphate, mueller-hinton, and todd-hewitt broths, using a logarithmic-phase inoculum of 10(5) colony-forming units per ml. minimal inhibitory concentrations in the three media did not differ significantly. however, minimal ... | 1979 | 384895 |
| [sensitivity of gram-negative microflora to lysozyme]. | sensitivity to lysozyme of the representatives of various species of gramnegative microflora (476 strains) was studied with a new modified procedure, which is more exact and economic as compared to the method of serial dilutions in agar. high resistance of eltor vibrio cholerae and pseudomonas to lysozyme was found. cultures of various sensitivity levels to lysozyme were detected among aeromonas, enteropathogenic e. coli and nag-vibrios. | 1979 | 386935 |
| analysis of mitotic nondisjunction with aspergillus nidulans. | two methods to detect the induction of nondisjunction with a diploid stable strain of a. nidulans are described. the first method gives only qualitative results, while the second method is quantitative and dose-effect curves can be done. some physiological parameters affecting the induction of nondisjunction can also be studied, because either quiescent or germinating conidia can be treated with the drug under test. some agents inducing nondisjunction were also tested for the induction of point ... | 1979 | 387402 |
| [instrument for sampling silt in sanitary-microbiologic studies]. | | 1979 | 387528 |
| artificial salmonella vaccines: o-antigenic oligosaccharide-protein conjugates induce protection against infection with salmonella typhimurium. | outbred mice were vaccinated with various artificial salmonella vaccines and subsequently challenged intraperitoneally with graded doses of virulent salmonella typhimurium. the salmonella vaccines used were: (i) octasaccharide, obtained by hydrolysis of the o-antigenic polysaccharide chain of s. typhimurium strain sh 4809 with phage p22-associated endo-rhamnosidase and covalently linked to either diphtheria toxin or edestine; (ii) purified outer membrane proteins (porins) from s. typhimurium; an ... | 1979 | 387597 |
| synthesis and properties of fluorescent nucleotide substrates for dna-dependent rna polymerases. | a new class of fluorescent nucleotide analogs which contain the fluorophore 1-aminonaphthalene-5-sulfonate attached via a gamma-phosphoamidate bond has been synthesized. both the purine and pyrimidine analogs have fluorescence emission maxima at 460 nm. cleavage of the alpha-beta-phosphoryl bond produces change in both the absorption and fluorescence emission spectra. the fluorescence of the pyrimidine analogs is quenched; cleavage of the alpha-beta-phosphoryl bond of the utp analog produces abo ... | 1979 | 387781 |
| oxygen-dependent cleavage of dna by the 1,10-phenanthroline . cuprous complex. inhibition of escherichia coli dna polymerase i. | | 1979 | 387784 |
| api and minitek systems in identification of clinical isolates of anaerobic gram-negative bacilli and clostridium species. | a comparison of the api and minitek methods of biochemical testing was made on a variety of anaerobic bacteria. although api and minitek results were not compared to more standardized or conventional procedures of identification, multiple repeat testing of the two systems was done on routine clinical isolates and known organisms to determine (i) whether the reactions were reliably consistent, (ii) the ease of reading the two systems with respect to the frequency of questionable results, and (iii ... | 1979 | 387809 |
| primary plate identification of group a beta-hemolytic streptococci utilizing a two-disk technique. | a two-disk system is described which allows primary plate identification of group a beta-hemolytic streptococci. group a beta-hemolytic streptococci could be visualized on primary throat culture plates by using trimethoprim-sulfamethoxazole to inhibit normal flora. in the heavily inoculated area of trypticase soy agar plates containing 5% sheep blood, a 25-microgram/ml trimethoprim-sulfamethoxazole disk was placed contiguous to a 0.04-u bacitracin disk. a total of 259 throat specimens were exami ... | 1979 | 387811 |
| metabolism of arachidonic acid in ionophore-stimulated neutrophils. esterification of a hydroxylated metabolite into phospholipids. | [14c]arachidonic acid incubated with human neutrophils was esterified into phospholipids and triglycerides. stimulation of these labeled neutrophils with ionophore a23187 (2 microm) results in release of [14c]arachidonate from phospholipid and its metabolism to prostaglandin e2 and 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-hete), a lipoxygenase product. the released arachidonate is also metabolized to a polar lipid of unknown composition here disignated compound a. 5-hete was found to be rele ... | 1979 | 387819 |
| serum opsonic activity in rodent malaria: functional and immunochemical characteristics in vitro. | the functional and immunochemical characteristics of serum opsonic activity in rodent malaria were examined in the present study. schizont- and late trophozoite-enriched populations of plasmodium berghei-infected red blood cells (irbc) were isolated on a ficoll density-gradient and used in an in vitro phagocytosis system composed of serum and monolayer cultures of rat peritoneal macrophages. hyperimmune serum augmented the phagocytosis of irbc to a greater degree than did nonimmune serum. when e ... | 1979 | 387873 |
| [anti-tumor effects of picibanil on mouse skin squamous cell cancer (author's transl)]. | | 1979 | 387895 |
| scanning electron microscope observations of microvascular anastomosis in the rat carotid artery. | microvascular anastomosis is gaining increasing significance in the field of replantation and reconstructive surgery. although traumatic interference is minimized by microsurgery, thrombotic occlusions remain the major reason for clinical failure. with the improvement of suturing technique studies were directed towards the causes of failure using light and scanning electron microscopy. our study was focussed on the latter aspect. the left common carotid artery of 55 albino rats was transsected ... | 1979 | 387900 |
| terminal deoxynucleotidyl transferase as a hematopoietic cell marker. | terminal deoxynucleotidyl transferase (tdt) is an intracellular protein characteristic of certain primitive lymphocytes in normal thymus and bone marrow. it can be detected by enzyme assay or immunocytochemical staining. high levels of enzyme and increased numbers of tdt+ cells are found in some lymphoblastic leukemias and lymphomas. analysis for tdt thus provides a useful adjunct in the differential diagnosis of leukemia. | 1979 | 389309 |
| [inductions of adjuvant arthritis in rats and footpad swelling reaction in mice by injection with the purified cell wall fraction from in vitro grown mycobacterium lepraemurium hawaii (author's transl)]. | | 1979 | 389918 |