| distribution in the rat central nervous system of acceptor sub-types for dendrotoxin, a k+ channel probe. | dendrotoxin, a snake polypeptide that facilitates the release of neurotransmitters, is a putative ligand for certain voltage-dependent, rapidly-activating k+ channels. using a 125i-labelled derivative, the location of high-affinity acceptors for this toxin in the rat central nervous system was established by quantitative sheet film autoradiography. a widespread distribution of binding sites was observed, with high densities of acceptors being found in most gray matter regions and along nerve tra ... | 1989 | 2725862 |
| one millisecond of light suffices to suppress nighttime pineal melatonin synthesis in rats. | the effect of a single high-intensity light pulse with a duration of 1 ms on nighttime pineal activity of male sprague-dawley rats was investigated. 10 minutes after light exposure pineal n-actyltransferase activity and melatonin content were significantly reduced. these results show that the rat pineal is capable of responding to very short light flashes of high intensity. | 1989 | 2725948 |
| effect of cocaine and lidocaine on the development of kindled seizures. | the effect of a subconvulsive dose of cocaine or lidocaine on the development of kindling was studied in male long-evans rats. animals were divided into three groups and kindled by daily electrical stimulation of the pyriform cortex. fifteen minutes before each stimulation each animal received an intraperitoneal injection of either saline, 20 mg/kg cocaine hydrochloride, or 20 mg/kg lidocaine hydrochloride. following kindling the drug treatment was discontinued and the transfer of kindling to a ... | 1989 | 2727005 |
| [entrapping of hydrophobized plague capsular antigen into large unilamellar liposomes]. | the entrapping of a plague capsular antigen into the liposomes, prepared by the "reverse phase" evaporating method, was investigated. both the native antigen and the antigen modified by palmitoylchloride were used. the entrapping of the modified antigen into the liposomes was greater than that of the native one: 46.1 +/- 5.4% and 3.7 +/- 2.7%, respectively. the highest content of the antigen on the membrane surface was observed when using palmitoylized protein and liposomes with egg lecithin, ch ... | 1989 | 2728118 |
| [thymine- and thymidine-dependence of yersinia pestis mutants with altered thymidylate synthetase]. | thymine- and thymidine-dependent mutants of y. pestis strain ev-76 have been isolated and characterized. obtaining y. pestis thymine-dependent mutants in trimethoprim-containing media with full nutritional value in the presence of thymine and thymidine and the capacity of natural strains from the foci of infection in transcaucasia and mongolia to grow in such media indicate that y. pestis has gene tpp controlling thymidine phosphorylase, but this enzyme is strongly suppressed under normal condit ... | 1989 | 2728696 |
| [yersinia pestis l-forms in rodents and ectoparasites]. | y. pestis l-forms and bacterial forms persist in the body of great gerbils for 40 days. l-forms are poorly phagocytized and can persist in phagocytes for a long time. in guinea pigs immunized with vaccine ev, y. pestis antigen could be detected till day 160. an unstable l-form was isolated from ornithodoros mites 3 years after their experimental infection with y. pestis. bacterial forms persist in mites for 1-3 years. for 5 years y. pestis antigen is regularly detected in a high percentage of mi ... | 1989 | 2728699 |
| quantitation of antegrade and retrograde blood flow in the human aorta by magnetic resonance velocity mapping. | magnetic resonance velocity mapping was used in 24 normal subjects to study two-dimensional velocity profiles in the proximal and mid-ascending aorta, and to quantify both forward and reverse flow. the aortic flow measurements were validated by comparison with left ventricular stroke volume in all subjects and by comparison with pulmonary flow measurements in 12. agreement was good with standard errors of the estimate of 7.8 and 7.1 ml, and correlation coefficients of 0.93 and 0.95, respectively ... | 1989 | 2729051 |
| pathogenesis of naturally acquired bovine respiratory syncytial virus infection in calves: morphologic and serologic findings. | lesions in 32 calves that died or were euthanatized during the course of severe natural infection with bovine respiratory syncytial virus (brsv) are described. all calves had been dyspneic for 1 to 2 days. at necropsy, lesions that could be related to dyspnea included congested and cyanotic mucosae and widespread petechiae. the lungs had various lesions in the cranioventral (cv) and caudodorsal (cd) portions. the cv portion of the lungs was consolidated, firm, and edematous. histologically, the ... | 1989 | 2729714 |
| [study of trimethoprim sensitivity in strains of yersinia pestis isolated from various natural foci of plague]. | the effect of exogenic addition of purines and pyrimidines on trimethoprim sensitivity was studied in 134 vaccinal and virulent strains of the plague causative agent isolated at various periods in 12 natural plague foci. 74 strains proved to be sensitive to low concentrations of trimethoprim in the cultivation media irrespective of the presence or absence of thymine or thymidine in them. in this respect the strains differed from many other bacterial species which in the presence of thymidine or ... | 1989 | 2730213 |
| [inhibitor production, a stable feature of plague pathogen]. | application of the methods developed by the author to the plague causative agent isolated in six natural plague foci provided detection of its ability to synthesize a factor inhibiting the growth of the producing strain and cultures isolated from the same focus. in the strains isolated from these foci the inhibitor presence was stable on the agar consisting of fermentative casein hydrolysate and 0.6 per cent of lithium chloride when the indicator strains were incubated under definite temperature ... | 1989 | 2730214 |
| chemotherapeutic agents, xv. synthesis of 4-amino-3-pyrazolo-1,2,4-triazoles as antimicrobial agents. | 4-amino-3-hydrazino-5-h/methyl-1,2,4-triazole dihydrochlorides (2a,b) were obtained by cyclization of triamino-guanidine-hcl in formic and acetic acids, respectively. condensation of 2a with pyrazol-4-carbaldehydes in ethanol yielded 3a,b. cyclocondensation of 2a,b with dimethylmercaptocyanoacrylonitrile, methyl dimethylmercapto-cyanoacrylate, ethoxymethylenemalononitrile and ethyl ethoxymethylenecyanoacetate individually provided 4a-d and 5a-d, respectively. the hydrazones 6a,b were obtained by ... | 1989 | 2730291 |
| sarcolemmal integrity during ischaemia and reperfusion of the isolated rat heart. | the effects of ischaemia and reperfusion on sarcolemmal permeability were studied in the isolated rat heart using an ionic lanthanum probe technique. detection of increased permeability after exposure to short periods of ischaemia without accompanying reperfusion effects was achieved by employing a substrate-free, low calcium saline solution as vehicle for ionic lanthanum. quantifiable results showed a rapid increase in sarcolemmal permeability in the subendocardium after 15 min of ischaemia, wi ... | 1989 | 2730527 |
| in vivo dna cross-linking by cyclophosphamide: comparison of human chronic lymphatic leukemia cells with mouse l1210 leukemia and normal bone marrow cells. | alkaline elution was done on a variety of cells following cyclophosphamide (cy) treatment in vivo. cells used were l1210 leukemia, normal mouse bone marrow, and peripheral blood cells obtained from a patient with chronic lymphatic leukemia (cll). endpoints used were determination of single strand breaks, dna-dna interstrand and dna-protein cross-links. after treatment of mice with cy (4 mg/mouse), low levels of single strand breaks were observed in both l1210 leukemia and cdf1 normal bone marrow ... | 1989 | 2731167 |
| igg and igm responses in high and low antibody-selected lines of chickens. | kinetics of igg and igm as measured by 2-mercaptoethanol-resistant (mer) and susceptible (mes) antibodies to sheep erythrocytes, respectively, were determined as correlated responses in lines of chickens selected for high (ha) and low (la) antibody response to sheep erythrocytes. primary response patterns for total, mer, and mes antibody differed according to the genetic line. total antibody increased rapidly, peaked, and persisted at moderate levels in line ha, whereas both peak and persistency ... | 1989 | 2732455 |
| reflex regulation of the sphincter of oddi by intrapancreatic ductal pressure in the japanese monkey. | japanese monkey has a common ampullary channel. by a continuous injection of sterile saline (1 ml/min) into the pancreatic main duct, the pancreatic main ductal pressure increased from 8.4 +/- 1.0 (mean +/- sd) to a plateau level (16-25 mmhg) in 5 japanese monkeys. the frequency and amplitude of the slow wave in electromyography of the sphincter of oddi (so) were significantly decreased from 14 to 2/min and 0.06 to 0.04 mv (p less than 0.01), and the frequency of the spike component significantl ... | 1989 | 2732530 |
| [the mutagenic effect of polymorphonuclear leukocytes on yersinia pestis]. | as the result of in vitro experiments, y. pestis auxotrophic mutants have been obtained under the influence of polymorphonuclear lymphocytes obtained from guinea pigs, previously immunized with y. pestis strain ev. the mutagenic effect has been found to occur at minute 45 of phagocytosis. the control treatment of the bacteria with the lysate of neutrophils, homologous serum, penicillin (antibiotic-influenced selection) has not been found to lead to the appearance of auxotrophicity. these data su ... | 1989 | 2735164 |
| expression of the low calcium response in yersinia pestis. | pathogenic yersiniae undergo an established low calcium response (lcr) at 37 degrees c in ca2+-deficient media characterized by restricted growth with synthesis of lcr plasmid-encoded virulence functions. the latter include outer membrane peptides (yops) known to undergo pst plasmid-mediated post-translational degradation in yersinia pestis but not in enteropathogenic yersiniae lacking this plasmid. salient yops of y. pestis are shown here to be either maintained in the steady state or to exist ... | 1989 | 2739560 |
| glycaemic effects of traditional european plant treatments for diabetes. studies in normal and streptozotocin diabetic mice. | twelve plants used for the traditional treatment of diabetes mellitus in northern europe were studied using normal and streptozotocin diabetic mice to evaluate effects on glucose homeostasis. the plants were administered in the diet (6.25% by weight) and/or as decoctions or infusions in place of drinking water, to coincide with the traditional method of preparation. treatment for 28 days with preparations of burdock (arctium lappa), cashew (anacardium occidentale), dandelion (taraxacum officinal ... | 1989 | 2743711 |
| alternative routes for the establishment of surface polarity during compaction of the mouse embryo. | during the process of compaction, mouse 8-cell blastomeres flatten upon each other and polarize along an axis perpendicular to cell contacts. if the process of flattening is prevented, polarization can still occur, but does so in a lower proportion of cells than for control populations, and without the normal contact-directed orientation. we compared contact-directed and noncontact-directed processes to see if they involve common mechanisms. in nonflattened cells, surface polarization was favore ... | 1989 | 2744237 |
| [physiological remineralization of artificially decalcified monkey dentin under adhesive composite resin restoration]. | this study was planned to investigate the remineralization potential of the inner carious dentin layer preserved under adhesive resin restoration in comparison with that under cement filling. dentin caries models were artificially produced in vivo with acid on the class v cavity floors of 25 bilateral tooth pairs from 3 monkeys. thirteen cavity pairs were filled with adhesive composite and twelve pairs with polycarboxylate cement, 120 days and immediately before sacrificing the monkeys. the sect ... | 1989 | 2746106 |
| conditions required for detection of specimen-specific se-i secondary electrons in an analytical sem. | an analytical sem equipped with an above-the-lens detector, an in-the-lens specimen stage and a high brightness lab6 emitter was used to produce a specimen-specific, secondary electron-i (se-i) signal for recording edge brightness contrast with high intensity on small particles at high magnification (200,000). the se-i edge brightness contrast produced from 20-40 nm colloidal gold on silicon wafers was useful for estimating instrument resolution since the edge brightness is the sum of the se-i s ... | 1989 | 2746638 |
| [results of screening of plasmids of yersinia pestis strains from various regions of endemic central-asian plague focus]. | the results of the plasmid screening of yersinia pestis strains isolated from four autonomous focuses on the northern border of the central asian zone of plague natural focality are presented. the plasmid profile of yersinia pestis strains from the focuses is characterized as stable and independent of the source and time of strain isolation. the peculiar characteristic of the strains isolated in tuva is the presence of an "additional" 15-16 md plasmid in those strains. | 1989 | 2747698 |
| resiniferatoxin, a phorbol-related diterpene, acts as an ultrapotent analog of capsaicin, the irritant constituent in red pepper. | resiniferatoxin is an extremely irritant diterpene present in the latex of several members of the genus euphorbia. its mechanism of action has been shown to be clearly distinct from that of the structurally related phorbol esters. since resiniferatoxin possesses a 4-hydroxy-3-methoxyphenyl substituent, a key feature of capsaicin, the major pungent ingredient of plants of the genus capsicum, we examined the ability of resiniferatoxin to induce typical capsaicin responses. we report here that trea ... | 1989 | 2747924 |
| effects of dobutamine and acetylcholine on perfused hearts isolated from streptozocin-induced diabetic rats. | in order to study the responsiveness of the diabetic heart to autonomic agents, effects of dobutamine (dob) and acetylcholine (ach) on perfused hearts isolated from streptozocin (streptozotocin, stz)-induced diabetic rats and insulin-treated diabetic rats were evaluated. male sprague-dawley rats, weighing 180-210 g, were divided into control (c) group, diabetes mellitus (dm) group, and diabetes mellitus treated with insulin (dmi) group. c group was injected with buffered vehicle. dm and dmi grou ... | 1989 | 2751733 |
| coupling of proteolytic quenching and high-performance liquid chromatography to enzyme reactions. application to bovine pancreatic ribonuclease. | | 1989 | 2753946 |
| treatment of acute traumatic internal carotid artery occlusion with extracranial-to-intracranial arterial bypass: case report. | ligation of the cervical internal carotid artery resulted in an acute neurological deficit in the dominant hemisphere of a 35-year-old man who suffered a penetrating injury to the neck. regional cerebral hypoperfusion was suspected because the ischemic symptoms occurred while the patient was fully heparinized. immediate institution of a barbiturate coma, volume expansion, and placement of a high-flow extracranial-intracranial arterial bypass graft led to rapid recovery of hemispheric function. h ... | 1989 | 2755586 |
| effect of dietary aluminum on calcium and phosphorus metabolism and performance of laying hens. | the effect was studied of feeding dietary al as aluminum sulfate on calcium and phosphorus metabolism and performance of single comb white leghorn laying hens. in trial 1, graded levels of al (0, .05, .10, or .15%) were added to a corn-soybean meal layer diet containing 3.3% ca and .5% available p. each diet was fed for 28 days to six groups of six individually caged hens. adding .15% al to the diet significantly (p less than .05) reduced feed intake, egg production, body weight, tibia breaking ... | 1989 | 2755896 |
| efficacy of fertilization in artificially inseminated turkey hens. | research was conducted to develop an artificial insemination protocol optimizing the use of spermatozoa by turkey breeder hens. large white turkey hens were inseminated on days 14 and 17 postphotostimulation with 200 million spermatozoa from one male phenotype to fill the oviductal storage sites. artificial inseminations were then performed weekly for 20 wk with different spermatozoa numbers of another male phenotype. fertility and phenotype of each poult were determined at hatch to ascertain wh ... | 1989 | 2755899 |
| experimental transplantation gliomas in the adult cat brain. 3. regional biochemistry. | experimental brain tumours were produced in adult cats by stereotactic xenotransplantation of the rat glioma clone f98. regional atp, glucose and lactate were measured after 2-4 weeks on coronal cryostat sections by substrate-induced bioluminescence, potassium content was imaged by the histochemical sodium cobaltinitrite method, and regional ph by incubating cryostat sections with the fluorescent ph-indicator umbelliferone. the regional biochemical alterations were correlated with magnetic reson ... | 1989 | 2756853 |
| thin-layer chromatographic scanner, spectrophotometric and high-performance liquid chromatographic methods for the determination of colchicine. | one high-performance liquid chromatographic (hplc) and two thin-layer chromatographic (tlc) methods are proposed for the determination of colchicine in crude drugs and pharmaceutical preparations. the tlc scanner method is based on measurement of the absorbance of the separated colchicine spot; alternatively, after scraping the spot from the plate and elution the absorbance can be measured spectrophotometrically. the hplc assay was carried out isocratically on a reversed-phase column using meoh- ... | 1989 | 2757228 |
| age changes in the crystallinity of bone mineral and in the disorder of its crystal. | the crystallinity of bone mineral and disorder of the crystal at different ages were measured by the x-ray diffraction method of ruland. measurements were made on femoral mid-diaphyses of wistar rat from 2 weeks to 1 year of age. for a given animal age, the crystallinity of bone mineral increases with age, while the overall disorder of the crystal does not vary within experimental accuracy. the increase in the crystallinity with age is attributed to an increase in crystallite size, a decrease in ... | 1989 | 2758062 |
| auditory and visual neurons in the cat's superior colliculus selective for the direction of apparent motion stimuli. | in the cat, cells of the superior colliculus (sc) and the tectofugal pathways of the visual system are highly selective for the direction of a moving visual stimulus. deep layer units of sc in addition respond to auditory and somatosensory stimuli, but the proportion of such non-visual cells is usually found to be much lower than that of visual cells. we recorded the responses of 174 cells in the sc to sequentially presented, localized visual and/or auditory stimuli that produced the sensation o ... | 1989 | 2758330 |
| extended spectrum cephalosporins and clostridium difficile. | there is little information about how commonly the newer cephalosporins cause diarrhoea due to clostridium difficile. in this study of 111 patients with c. difficile-associated diarrhoea, 106 had received antimicrobial agents in the four weeks before detection of c. difficile. the relative risk for each antimicrobial agent was greatest with clindamycin, followed by cefotaxime, cephamandole and ceftriaxone. there was no statistically significant difference in risk between the cephalosporins evalu ... | 1989 | 2759933 |
| analysis of the yopa gene encoding the yop1 virulence determinants of yersinia spp. | the yop proteins of yersinia are important virulence determinants. the yop1 protein sequences of yersinia pestis, yersinia pseudotuberculosis, and two yersinia enterocolitica serotypes, 0:3 and 0:8, deduced from the nucleotide sequences of the corresponding yopa genes, were compared. most differences were found in the hydrophilic domains of the proteins, whereas the hydrophobic domains were conserved. the amino acid sequences revealed a signal sequence 25 amino acids long. no cysteine residues w ... | 1989 | 2761389 |
| cardiac adaptations to aortic constriction in adult and aged rats. | myocardial function in vitro and myosin heavy chains (mhcs) were studied in control and hypertrophied hearts of adult (9-10 mo) and aged (25-28 mo) female fischer 344 rats 7 days after aortic constriction. aortic constriction increased left ventricular mass to 110 and 112% of the control values of 484 +/- 12 and 617 +/- 18 (se) mg in adult and aged rats, respectively. after aortic constriction, there was a significant age-related difference in the adaptation of peak pressure development in vitro ... | 1989 | 2764145 |
| experimental atherosclerosis and oxygen free radicals. | oxygen free radicals are known to produce cellular injury by peroxidation of phospholipids in the cell membrane. these free radicals might damage the endothelial cell and thus set the stage for atherosclerosis. the authors studied the effect of high-cholesterol diets on the genesis of atherosclerosis and lipid peroxidation products, malondialdehyde (mda) in rabbits. the animals were divided into four groups each comprising 5 rabbits, on the basis of their diets. group i, control diet; group ii, ... | 1989 | 2764311 |
| effects of co-administration of di(2-ethylhexyl)phthalate and testosterone on several parameters in the testis and pharmacokinetics of its mono-de-esterified metabolite. | the administration of 1 g/kg di(2-ethylhexyl)phthalate (dehp) or 5 mg/kg testosterone for 1 week did not affect the testicular and prostatic gland weights in rats. however, co-administration of dehp and testosterone induced severe testicular atrophy accompanied by a decrease of zinc concentration in the testis and reduction of the activity of testicular specific lactate dehydrogenase isozyme. these changes were similar to the results of high dose administration of dehp alone. values of biologica ... | 1989 | 2764717 |
| formation of hydroxy derivatives, aldehydes, and nitrite from n-nitrosomethyl-n-amylamine by rat liver microsomes and by purified cytochrome p-450 iib1. | the metabolism was examined of the esophageal carcinogen n-nitrosomethylamylamine (nmaa) by liver microsomes and slices from adult male sprague-dawley rats. hydroxylation at c-2 to c-5 of the amyl group to give stable hydroxy-nmaas was studied by gas chromatography-thermal energy analysis to determine the products. microsomal metabolism produced mainly 4-hydroxy-nmaa, proceeded optimally in 100 mm phosphate at ph 7.4, and showed no sex differences. induction by phenobarbital (pb) and 3-methylcho ... | 1989 | 2766298 |
| endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium. | the inhibitory effects of the endothelium against ergonovine-induced contraction were examined in isolated porcine coronary arteries under normal conditions and after endothelial regeneration. endothelium-dependent responses were examined in vitro in normal yorkshire pigs (n = 16) and in pigs that had undergone balloon endothelium removal of the left anterior descending coronary artery (lad) 4 weeks before the study (n = 10). the presence of a complete endothelial lining was confirmed histologic ... | 1989 | 2766515 |
| kinetics of myoglobin release and prediction of myocardial myoglobin depletion after coronary artery reperfusion. | to better define the usefulness of blood myoglobin measurements in evaluating the effectiveness of attempted thrombolysis, we studied the kinetics of myoglobin entry into and removal from the circulation after coronary artery reperfusion and the relation between directly measured depletion of myocardial myoglobin after coronary occlusion and reperfusion and the amount of depletion predicted from plasma myoglobin concentration-time curves. initially, canine myoglobin was administered to 11 dogs b ... | 1989 | 2766516 |
| treatment of childhood bacterial meningitis with ceftriaxone once daily: open, prospective, randomized, comparative study of short-course versus standard-length therapy. | fifty-two children were included in this study to evaluate and compare short- versus standard-length ceftriaxone therapy for bacterial meningitis. the duration of the short-course regimens was 4, 6 and 7 days for neisseria meningitidis, hemophilus influenzae and streptococcus pneumoniae, respectively. the standard-length regimens were twice as long. on the basis of a computer-generated randomization list, 26 children were assigned either to the short- or to the standard-treatment regimen. ceftri ... | 1989 | 2766869 |
| use of dna hybridizations probes for detection of the plague bacillus (yersinia pestis) in fleas (siphonaptera: pulicidae and ceratophyllidae). | the detection of active plaque in nature relies primarily on demonstration of the etiologic agent of the disease. yersinia pestis, in the flea vectors and susceptible mammalian hosts. a live animal assay is currently used for identification of a y. pestis virulence antigen that is not expressed in the flea. we have found that dna hybridization probes specific for y. pestis, used in very simple sample preparation schemes, allow detection of y. pestis in three species of fleas as well as tissues o ... | 1989 | 2769715 |
| [a young man with pneumonia with a torpid course]. | | 1989 | 2772353 |
| community-acquired pneumonia requiring hospitalization: 5-year prospective study. | we studied all patients with community-acquired pneumonia who were admitted to our 800-bed adult acute care hospital from 1 november 1981 to 15 march 1987. the 719 patients had a mean age of 63.2 years; 18% were admitted from nursing homes, and 18% required ventilatory assistance as part of the therapy for pneumonia. patients with nursing home-acquired pneumonia were significantly older; had a higher mortality (40% vs. 17%); were more likely to be admitted in january; were less likely to complai ... | 1989 | 2772465 |
| pseudomonas cepacia typing systems: collaborative study to assess their potential in epidemiologic investigations. | to determine the utility of available pseudomonas cepacia typing systems for confirming the relatedness of isolates, we applied these methods to isolates associated with previously investigated nosocomial outbreaks. we compared chromosome analysis, serologic reactions, biochemical tests, bacteriocin production and susceptibility, and antimicrobial susceptibility in their ability to determine outbreak relatedness. chromosome analysis, serologic reactions, and biochemical tests were each demonstra ... | 1989 | 2772466 |
| reconstruction of the four major ligaments in an unstable knee joint after dislocation by solvent-preserved human fascia lata transplantation. a case report. | once the opportunity for primary repair of injured knee ligaments after traumatic dislocation has been lost, ligamentous reconstruction is difficult using only autogenic tissues because of the risk of loss of function at the donor site, so other substitutes are needed. the four major ligaments in the unstable knee of a 35-year-old man were reconstructed by solvent-preserved human fascia lata three months after traumatic open dislocation. the clinical results were satisfactory. arthroscopic exami ... | 1989 | 2774879 |
| suppression of antibody responses to the acetylcholine receptor by natural antibodies. | some relationships between "natural" physiological autoreactivity and antibody responses against the acetylcholine receptor (achr) were investigated. collections of unselected monoclonal antibodies were derived from nonmanipulated newborn mice of susceptible (balb.b) and resistant (balb/c) mouse strains, and screened for reactivity with heterologous achr. five in 200 balb/c but none in 200 balb.b clones were found reactive. four of these anti-achr antibodies were analyzed for connectivity to nin ... | 1989 | 2776827 |
| the mrna for alpha 1-acid glycoprotein is deadenylated during stimulation by dexamethasone. | | 1989 | 2780624 |
| opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony. | | 1989 | 2780954 |
| induction of cytochromes p450iie1 and p450iib1 by secondary ketones and the role of p450iie1 in chloroform metabolism. | it has been shown previously that the potentiation of chloroform-induced hepatotoxicity by linear secondary ketones increases with the carbon-chain length. the present work examines the possibility that this potentiation is due to the induction of p450iie1. the metabolism of chloroform, as measured using headspace gas chromatography, in the presence of microsomes from acetone-treated rats was elevated threefold compared to controls. inclusion of monoclonal antibody against p450iie1 inhibited the ... | 1989 | 2781561 |
| modulation of il-2-induced human b cell proliferation in the presence of human 50-kda b cell growth factor and il-4. | several human t cell derived factors capable of stimulating human b cells to synthesize dna have been previously described. one such factor exhibits an apparent m.w. of 50,000 da and has been termed 50-kda-b cell growth factor (bcgf). in this report, we show that a human b cell proliferation pathway based on the sequential action of anti-mu antibody, 50-kda-bcgf and il-2 is inhibited in the presence of human ril-4. although il-4 itself is capable of triggering b cell dna synthesis as measured at ... | 1989 | 2783328 |
| a monoclonal antibody to human b lymphoblastoid cells activates human and murine t lymphocytes. | a b lymphoblastoid cell line can provide a comitogenic, accessory signal for mitogen-treated t cells. in a study evaluating the antigenic determinant of such cells that mediate this effect, a monoclonal antibody (i57) was raised against the daudi cell line. this antibody was found to interact with a 30-kda protein on these cells and had agonistic properties. it enhanced the b lymphoblastoid accessory cell and interleukin 1 (il-1)-dependent stimulation of pha-treated murine thymocytes. the stimul ... | 1989 | 2783390 |
| alpha 1 chain of chick type vi collagen. the complete cdna sequence reveals a hybrid molecule made of one short collagen and three von willebrand factor type a-like domains. | a cdna library constructed from chick aorta poly(a+) rna in the expression vector pex1 was screened with rabbit polyclonal antisera. additional clones were obtained by dna-dna hybridization with subclones from the most 5'- and 3'-ends. the overlapping clones span 4.6 kilobases and code for the entire alpha 1 (vi) chain. the nucleotide sequence reveals a 3057-base pair open reading frame that codes for 1019 amino acids. analysis of the deduced amino acid sequence predicts that alpha 1 (vi) has on ... | 1989 | 2784434 |
| interleukin 4 acts on both high- and low-density murine b cell subpopulations to induce ige and igg1 synthesis in vitro. | murine b cells were activated for 24 h with either lipopolysaccharide (lps) or polyribosyl-ribitol-phosphate (prp), followed by addition of interleukin 4 (il-4) and the immunoglobulin isotypes secreted into the supernatant quantitated. without il-4, both lps and prp induced mainly igm and igg3 and no ige secretion. addition of il-4 to both lps- and prp-activated cells decreased the igm and igg3 secretion and induced a large igg1 production. in contrast to igg1, only lps-activated cells secreted ... | 1989 | 2785084 |
| mouse fetal thymus lobes cultured in il-2 generate cd3+, tcr-gamma delta-expressing cd4-/cd8+ and cd4-/cd8- cells. | whole, undisrupted, 14-day fetal mouse thymus lobes were liquid-cultured in medium supplemented with 10 u/ml ril-2. the cells that emerged from these fetal thymus lobes grew in an il-2-dependent manner and showed non-mhc-restricted cytolytic activity. by two-color flow microfluorimetric analysis they could be subdivided into cd4-/cd8+ and cd4-/cd8- subpopulations, both of which contained cd3+ cells. northern analysis of total cellular rna revealed full-length transcripts of gamma- and delta-mess ... | 1989 | 2785551 |
| two cases of pneumocystis carinii pneumonia occurred in human immunodeficiency virus infected patients: supplemental treatment with aerosolised pentamidine isethionate. | two cases of pneumocystis carinii pneumonia occurred in human immunodeficiency virus infected patients who were diagnosed by the aspiration-lung-biopsy. they were treated with 4 mg/kg of pentamidine isethionate intravenously administered followed by pentamidine aerosol inhalation. although clinical and laboratory findings were improved by the fifth or seventh day of intravenous therapy, substantial leukocytopenia, from 3,000/cmm to 600/cmm and from 2,700/cmm to 1,000/cmm, occurred. at this point ... | 1989 | 2786100 |
| protection induced against acute bronchitis--the use of human and rat models to determine mechanisms of action of oral immunization with haemophilus influenzae. | | 1989 | 2786462 |
| human t lymphocytes possess a limited in vitro life span. | the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ... | 1989 | 2786475 |
| eimeria tenella and e. acervulina: lymphokines secreted by an avian t cell lymphoma or by sporozoite-stimulated immune t lymphocytes protect chickens against avian coccidiosis. | the role of avian lymphokines as nonspecific immunomodulators of host immunity against the intracellular parasite eimeria was investigated. prophylactic treatment of normal chickens with crude cell-free supernatants obtained from jmv-1 culture, concanavalin a (con a)-stimulated normal spleen cells, or sporozoite-stimulated immune t cells prior to inoculation with e. tenella or e. acervulina conferred significant protection. these crude cell-free culture supernatants also inhibited intracellular ... | 1989 | 2786476 |
| enhanced antitumor effect of combination therapy with interleukin-2 and polyribonucleotides against adenocarcinoma 755. | human recombinant interleukin-2 (il-2) and interferon (ifn) inducers in combination were evaluated for their in vivo antitumor efficacy in relation to s.c.-implanted adenocarcinoma 755 in c57bl/6 mice. two ifn inducers, polyinosinic-polycytidylic acid [poly(i)poly(c)] and polyadenylic-polyuridylic acid [poly(a)poly(u)], induced high ifn production in various tissues for a long time compared to treatment with murine interferon-beta. especially, poly(i)poly(c) at 5 mg/kg, the maximum tolerated dos ... | 1989 | 2786554 |
| inhibition of t cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of t cells to blocking. | synthetic peptides corresponding to sequences 46-62 and 51-62 of mouse lysozyme and 46-61 of hen egg-white lysozyme (hel) were used as competitors in a variety of t cell responses. the competitors, according to their binding specificity for major histocompatibility complex (mhc) were expected to inhibit t cell responses restricted to i-ak, but not those restricted to i-ad, i-ek molecules. in competition experiments with t cell hybridomas, the poor binder i-ed molecule required 10- to 15-fold hig ... | 1989 | 2787751 |
| the colour centre in the cerebral cortex of man. | anatomical and physiological studies have shown that there is an area specialized for the processing of colour (area v4) in the prestriate cortex of macaque monkey brain. earlier this century, suggestive clinical evidence for a colour centre in the brain of man was dismissed because of the association of other visual defects with the defects in colour vision. however, since the demonstration of functional specialization in the macaque cortex, the question of a colour centre in man has been reinv ... | 1989 | 2787893 |
| interleukin 1 in rheumatoid arthritis: potentiation of immune responses within the joint. | specific immunoassays were used to measure il-1 peptides in the serum and synovial fluid of patients with rheumatoid arthritis (ra) and in the serum of age-matched healthy controls. patients with ra had raised levels of both il-1 beta and il-1 alpha in their sera compared to controls. synovial fluid levels of il-1 beta significantly correlated with immunoreactive il-2 and soluble il-2 receptor (sil-2r). in addition, incubation of synovial fluid mnc with human recombinant (hr) il-1 caused a dose- ... | 1989 | 2789325 |
| potentiation by vestibular stimulation of the immobility reflex elicited by clamping in developing and adult rats. | we have previously described (exp. neurol., 97: 315-326, 1987) that clamping of the neck elicits a profound immobility with flexion of limbs and ventroflexion of head and neck ('carrying posture'). we also observed that when a clamped rat pup is carried by the experimenter's hand, such carrying posture was potentiated. in the present paper we observed that in adult rats vestibular stimulation by circular acceleration potentiates the duration of immobility reflex by clamping and intensifies the l ... | 1989 | 2789702 |
| squamous cell carcinoma of the base of the tongue: results of treatment in 115 cases. | between 1976 and 1986, we treated 115 patients (mean age 53.8 years) with base of tongue carcinomas. the staging system used was the uicc tnm classification of 1979. seventy per cent of the tumours were t3 or t4 and 42% had n2 or n3 lymph node. locoregional treatment was irradiation alone (98/115) or surgery and post-operative radiotherapy (17/115). sixty-seven patients received induction chemotherapy. actuarial survival of the entire group at 3 and 5 years was 25 and 23%, respectively, and 3-ye ... | 1989 | 2790426 |
| [determination of nifedipine and its metabolites in plasma using gas chromatography]. | a method was worked out to determine nifedipine and its metabolite in plasma with the use of the packed chromatographic column 3% ov-101 and the detector of electron capture. the adjustment od plasma is simple. it consists in denaturation of plasma proteins and subsequent simple extraction with benzene. denaturation of proteins present in canine plasma is carried out with methanol, where as denaturation of human plasma with ammonium phosphomolybdate. the minimal detectable amount of nifedipine i ... | 1989 | 2790988 |
| [clinical and experimental research on a kidney-tonifying prescription in preventing and treating children's hearing-loss induced by aminoglycoside antibiotic ototoxicity]. | this paper reports 30 cases of hearing-loss induced by aminoglycoside antibiotic ototoxicity treated mainly with kidney-tonifying herbs such as herba epimedii, rhizoma drynariae, rhizoma polygonati, radix polygoni multiflori, magnetitum and rhizoma acori graminei, etc. after three month's treatment, the authors found that 19 cases had improved their hearing more than 15 db, among which there are 8 cases who had improved 15 db and 11 cases 30 db. the patients' hearing of pre-treatment and post-tr ... | 1989 | 2791158 |
| [the effect of intensification of an hiv viral infection with monoclonal antibodies]. | | 1989 | 2791870 |
| regulation of expression of the messenger ribonucleic acid encoding the cytosolic form of phosphoenolpyruvate carboxykinase in liver and small intestine of lactating rats. | the content of p-enolpyruvate carboxykinase enzyme mrna (mrna(pepck)) in the liver and small intestine changes in a coordinate way at various stages of pregnancy, lactation, and weaning periods of rats. on the ninth day of lactation, the accumulation of mrna(pepck) in the liver and small intestine was 82% and 152%, respectively, compared with that in starved control rats. after 18 h of induced weaning, both tissues presented residual levels of mrna(pepck). however, after 60 h of induced weaning, ... | 1989 | 2792001 |
| estradiol-stimulated increases in uterine eosinophils and nuclear type ii estrogen-binding sites are prevented by pertussis toxin. | previous studies from several laboratories have demonstrated that estradiol treatment resulted in an increase in nuclear type ii binding sites. our previous data suggest that this increase was due to the estradiol-stimulated influx of circulating eosinophils. therefore, we suggested that the uterine nuclear type ii estrogen-binding sites were not of uterine origin. in this report we present further evidence to support this hypothesis. treatment of immature rats with estradiol resulted in the sti ... | 1989 | 2792007 |
| thrombocytopenia associated with the induction of neonatal tolerance to alloantigens: immunopathogenic mechanisms. | balb/c mice rendered tolerant to alloantigens by neonatal injection of semi-allogeneic (c57bl/6 x balb/c)f1 spleen cells develop a thrombocytopenia in association with an autoimmune lupus-like syndrome. the possible mechanisms involved in the thrombocytopenia were investigated. the development of thrombocytopenia was first detected at 3 weeks of age coinciding with the start of the other autoimmune manifestations and was always related to a state of tolerance and b cell chimerism. there was a si ... | 1989 | 2792184 |
| biochemical and clinical response of fulminant viral hepatitis to administration of prostaglandin e. a preliminary report. | the effect of pg on patients with fulminant and subfulminant viral hepatitis (fhf) was studied. 17 patients presented with fhf secondary to hepatitis a (n = 3), hepatitis b (n = 6), and non-a, non-b (nanb) hepatitis (n = 8). 14 of the 17 patients had stage iii or iv hepatic encephalopathy (he). at presentation the mean aspartate transaminase (ast) was 1,844 +/- 1,246 u/liter, bilirubin 232 +/- 135 mumol/liter, prothrombin time (pt) 34 +/- 18, partial thromboplastin time (ptt) 73 +/- 26 s, and co ... | 1989 | 2794044 |
| evidence for an agonist-induced, atp-dependent change in muscarinic receptors of intact 1321n1 cells. | the binding of muscarinic agonists, partial agonists and antagonists to muscarinic receptors of intact 1321n1 human astrocytoma cells and of cell lysates was studied. partial agonists and antagonists exhibited similar apparent affinities in intact cell competition binding assays with either the lipophilic radioligand [3h]quinuclidinyl benzilate [( 3h]qnb) or the hydrophilic radioligand [3h]n-methyl scopolamine [( 3h]nms). in contrast, full agonists exhibited markedly lower apparent affinities in ... | 1989 | 2795471 |
| effect of d-ala-2-me-phe-4-gly-ol-5 enkephalin on epinephrine-induced arrhythmias in the rat and the interrelationship to the parasympathetic nervous system. | the purpose of this study was to evaluate the effects of the millimicrons opioid agonist d-ala-2-me-phe-4-gly-ol enkephalin (dago) on catecholamine-induced arrhythmias. arrhythmias were produced, in the rat, by continuous infusion of epinephrine until the development of fatal arrhythmias that were usually ventricular fibrillation. intracerebroventricular (icv) administration of dago, 3 nmol, significantly (p less than 0.05) shifted to the right the relationship between epinephrine and both the o ... | 1989 | 2796596 |
| detection of 3-hydroxy-3-methyloxindole in human urine. | during routine toxicological screening of urine for possible drug overdose, using two-dimensional thin-layer chromatography, an unknown substance was periodically detected that could not be related to any known drug. the substance was mass-isolated from the urine of a schizophrenic patient, who excreted it prolifically and it was chemically identified as 3-hydroxy-3-methyloxindole by mass spectrometry and 1h- and 13c-nmr. the structure was confirmed by synthesis through methylation of isatin. th ... | 1989 | 2796599 |
| a case report of chediak-higashi syndrome complicated with systemic amyloidosis and olivo-cerebellar degeneration. | the histopathological observations in the case of a 24 year old woman with chediak-higashi syndrome are described. there were characteristic features of cytoplasmic giant granules in various cells and lymphohistiocytic infiltration in various tissues. amyloid deposits, which have not been reported previously in human chediak-higashi syndrome, were systemically noted and were immunohistochemically revealed to be aa type protein. another rare complication, olivo-cerebellar degeneration, was observ ... | 1989 | 2798223 |
| a case report of chediak-higashi syndrome complicated with systemic amyloidosis and olivo-cerebellar degeneration. | the histopathological observations in the case of a 24 year old woman with chediak-higashi syndrome are described. there were characteristic features of cytoplasmic giant granules in various cells and lymphohistiocytic infiltration in various tissues. amyloid deposits, which have not been reported previously in human chediak-higashi syndrome, were systemically noted and were immunohistochemically revealed to be aa type protein. another rare complication, olivo-cerebellar degeneration, was observ ... | 1989 | 2798223 |
| short-term primary culture of rat prostate tumor epithelial cells on reconstituted basement membrane as a useful in vitro model to assess drug action on prostatic cancer: efficacy of the thiazolidinedione derivative cgp 19984. | a model system has been developed that permits short-term culture of rat r3327 prostate adenocarcinoma epithelial cells on a reconstituted basement membrane. growth of prostate tumor cells under these conditions resulted in an enriched epithelial cell population that exhibited an eightfold increase in cell number in 10 days. this model system was used to test the efficacy of the thiazolidinedione derivative cgp 19984, a drug that inhibits luteinizing hormone secretion in vivo. at concentrations ... | 1989 | 2798233 |
| short-term primary culture of rat prostate tumor epithelial cells on reconstituted basement membrane as a useful in vitro model to assess drug action on prostatic cancer: efficacy of the thiazolidinedione derivative cgp 19984. | a model system has been developed that permits short-term culture of rat r3327 prostate adenocarcinoma epithelial cells on a reconstituted basement membrane. growth of prostate tumor cells under these conditions resulted in an enriched epithelial cell population that exhibited an eightfold increase in cell number in 10 days. this model system was used to test the efficacy of the thiazolidinedione derivative cgp 19984, a drug that inhibits luteinizing hormone secretion in vivo. at concentrations ... | 1989 | 2798233 |
| presence of cytoadhesins (iib-iiia-like glycoproteins) on human metastatic melanomas but not on benign melanocytes. | glycoproteins iib and iiia, a heterodimer complex, play a vital role in blood platelet aggregation and are members of a wide family of membrane receptors known as integrins or cytoadhesins. cellular interaction to extracellular matrix (ecm) adhesive proteins is mediated by integrins. certain tumor cells are known to interact with ecm and blood platelets in the process of metastasis. however, it is not known if tumor cells, compared with their normal counterparts, acquire iib-iiia-like receptors ... | 1989 | 2801613 |
| effect of acidic ph on the protein carmin from safflower seed (carthamus tinctorius). | the effect of a decrease in ph on the structural integrity of carmin has been monitored by a variety of biophysical techniques. the protein undergoes initial dissociation up to ph 3.5-4.0 without any significant denaturation. below this ph the process of dissociation and denaturation appears to be simultaneous. further, in the ph range of 2.5-1.6 the protein reassociates to probably a different polymer resulting from possibly, an entropically driven hydrophobic interaction. the process of dissoc ... | 1989 | 2803516 |
| steroidogenesis in liposomal system containing adrenal microsomal cytochrome p-450 electron transfer components. | purified adrenal microsomal p-450c21 and/or p-45017 alpha,lyase were incorporated with purified nadph-cytochrome-p-450 reductase into liposome membranes composed of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine at a molar ratio of 5:3:1. the rate dependences of reduction of liposomal p-450c21 in the fast phase as well as progesterone hydroxylation activities of p-450c21 and p-45017 alpha,lyase on the reductase concentration in the liposome membranes suggested that electron ... | 1989 | 2804111 |
| ultrastructure of globule leucocytes isolated from rat tracheal epithelium. | the ultrastructure of globule leucocytes isolated from rat tracheal epithelium is described. the isolated globule leucocytes appear as rounded mononuclear cells with a diameter of 6 to 8 microns. the often indented nucleus is usually eccentrically located in the cell. the characteristic intracytoplasmic membrane-bound granules vary greatly in electron-density, size, shape, and internal structure. on the basis of their internal structure, four types of granules could be distinguished. they may re ... | 1989 | 2804960 |
| purification of a vasodilator-regulated phosphoprotein from human platelets. | cyclic-nucleotide-elevating vasodilators such as prostaglandin e1, prostacyclin, sodium nitroprusside and endothelium-derived relaxing factor inhibit both contraction of vascular smooth muscle cells and the aggregation of platelets at an early step of the activation cascade. previous studies from this laboratory [waldmann, r., nieberding, m. and walter, u. (1987) eur. j. biochem. 167, 441-448) established that in human platelets cyclic-nucleotide-elevating vasodilators stimulated a pattern of pr ... | 1989 | 2806262 |
| some remarkable effects of thiopeptide and derived linkages on lysozyme release from neutrophils by esters of the chemotactic peptide n-formyl-methionyl-leucyl-phenylalanine (f-met-leu-phe-or). | a variety of recently synthesized analogues of the chemotactic agent f-met-leu-phe-or modified in the backbone were tested for their ability to induce the release of lysozyme from human neutrophils. in sharp contrast to the effects of thiopeptide linkages on the biological activity of leu5-enkephalin as previously reported, the presence of single thioamide bonds at either one of the endo-positions of the chemotactic peptide, abolished activity. thioamide-derived linkages such as amidoximes and c ... | 1989 | 2807625 |
| purification and characterization of the rat pancreatic cholecystokinin receptor. | cholecystokinin (cck) is a peptide hormone that has a variety of physiologically important functions in the gastrointestinal tract, in which distinct high affinity receptors have been identified. we describe here the purification of the digitonin-solubilized rat pancreatic receptor as an initial step in the determination of its primary structure. solubilization of total pancreatic membranes using 1% digitonin resulted in a single class of binding sites with a specific content of 4 pmol/mg as mea ... | 1989 | 2808360 |
| identification and characterization of a cell-surface molecule that is selectively induced on rat lymphokine-activated killer cells. | we have identified a 40- to 45-kda cell-surface molecule designated gp42, that is expressed in high levels by rat lymphokine-activated killer (lak) cells of nk cell origin. gp42 cannot be detected on the precursors of lak cells and is not present on resting or activated t cells. rather, expression of gp42 is selectively induced on nk cells by the high concentrations of ril-2 that are required for the induction of lak activity. although the function of gp42 is not known, the selective nature of i ... | 1989 | 2809220 |
| identification and characterization of a cell-surface molecule that is selectively induced on rat lymphokine-activated killer cells. | we have identified a 40- to 45-kda cell-surface molecule designated gp42, that is expressed in high levels by rat lymphokine-activated killer (lak) cells of nk cell origin. gp42 cannot be detected on the precursors of lak cells and is not present on resting or activated t cells. rather, expression of gp42 is selectively induced on nk cells by the high concentrations of ril-2 that are required for the induction of lak activity. although the function of gp42 is not known, the selective nature of i ... | 1989 | 2809220 |
| experimentally induced plague infection in the northern grasshopper mouse (onychomys leucogaster) acquired by consumption of infected prey. | in this study, 20 laboratory reared onychomys leucogaster from a parental population that is naturally exposed to plague were each fed a white mouse that had been inoculated with yersinia pestis. three of the 20 o. leucogaster died, four survived with antibody titers against y. pestis and 13 survived with no titer against y. pestis. in contrast, when 20 o. leucogaster from a plague naive parental population were fed infected prey, seven died and 13 survived with no antibody titer against y. pest ... | 1989 | 2810547 |
| [utilization of hemoglobin by the plague microbe]. | hemin and hemoglobin are found to be adequate sources of iron for growth of yersinia pestis. the mechanism of their assimilation is similar and consists of absorbtion of the free intact hem molecule. | 1989 | 2811899 |
| [transcription map of a recombinant plasmid carrying a gene for the synthesis of pesticine i and a protein for pesticine i immunity in the plague microbe]. | molecules of the plasmids pbr322 and prd17 have been compared by electron microscopy technique of r-loops visualization. comparative location of r-loops on the plasmids has been computerized on minicomputer hp9825a due to the program making possible to define the coordinates of the transcription start and direction. the 5.1 kb fragment coding for pesticin i and immunity protein to pesticin i and cloned in pbr322 vector plasmid is flanked by bam hi-ecori sites. five promoter regions and direction ... | 1989 | 2811901 |
| [genetic analysis and simulation of the virulence of yersinia pestis]. | the genetic analysis of y. pestis virulence factors accomplished in the 358 strain isogenic system allowed us to determine a minimal set of known factors providing pathogenicity. the combination of chromosomal marker pgm+ and calcium dependence plasmid (pcad) is shown to be sufficient for preserving the virulence of y. pestis. experimental modelling of virulence in this microorganism by the genetic exchange methods was carried out. the reduced virulence of the strains pgm+ and pcad+ for guinea p ... | 1989 | 2811911 |
| [how innocent is toxocara?]. | we have investigated 234 children (3-6 years of age) in the hague, the netherlands, for the presence of antibodies against toxocara. of these children 26 (11.1%) were seropositive to toxocara. cross-reactions with antibodies against ascaris could be excluded. of the seropositive children 31% had asthma or bronchitis, while this percentage was only 12% for the seronegative children. this might indicate that toxocariasis causes asthma or bronchitis in some children. if further investigations corro ... | 1989 | 2812077 |
| [how innocent is toxocara?]. | we have investigated 234 children (3-6 years of age) in the hague, the netherlands, for the presence of antibodies against toxocara. of these children 26 (11.1%) were seropositive to toxocara. cross-reactions with antibodies against ascaris could be excluded. of the seropositive children 31% had asthma or bronchitis, while this percentage was only 12% for the seronegative children. this might indicate that toxocariasis causes asthma or bronchitis in some children. if further investigations corro ... | 1989 | 2812077 |
| [a case of experimental transmission of the causative agent of plague from ornithodoros tartakovskyi ticks to fleas of the great gerbil]. | biological interrelations between ectoparasites from the colonies of great gerbil were studied experimentally. hungry fleas coptopsylla lamellifer are able to feed on the ticks ornithodoros tartakovskyi infected with plague and in so doing be infected with plague agent. such feeding is identified as "heterovampirism". | 1989 | 2812860 |
| quantitative analysis of protein mixtures by second derivative absorption spectroscopy. | a new method for the quantitative analysis of protein mixtures based on multicomponent analysis of the second derivative near uv spectra is described. using bovine eye lens crystallins, we demonstrate that the technique can provide precise concentrations of closely related proteins within mixtures, under both native and denaturing conditions. we have also successfully used the method to analyze the subunit composition of a heteromultimeric protein aggregate. the method is more rapid and precise ... | 1989 | 2817383 |
| quantitative analysis of protein mixtures by second derivative absorption spectroscopy. | a new method for the quantitative analysis of protein mixtures based on multicomponent analysis of the second derivative near uv spectra is described. using bovine eye lens crystallins, we demonstrate that the technique can provide precise concentrations of closely related proteins within mixtures, under both native and denaturing conditions. we have also successfully used the method to analyze the subunit composition of a heteromultimeric protein aggregate. the method is more rapid and precise ... | 1989 | 2817383 |
| [functional morphology of the maxillo-mandibular apparatus in the miniature swine mini-lewe. 11. discussion of the findings of the metric studies and dentition (conclusion)]. | the graphs showing the dimensions and angles of the skull and jaw indicate that postnatal development trends are basically uniform. comparison with the vietnamese miniature pig shows that the miniature pig mini-lewe has a longer skull. postnatal skull growth is accompanied by a distinct shift in proportions. the sequence of dentition if broadly identical to that of the domestic pig and the pitmann-moore miniature pig. | 1989 | 2817410 |
| use of nude (athymic) mice for the study of hypertrophic scars and keloids: vascular continuity between mouse and implants. | hypertrophic scars and keloids appear to be unique to humans since animals are not known to form these lesions. therefore, in an effort to develop an experimental model for their study, implants of these human lesions were made in nude (athymic) mice (nu/nu) in suprascapular subcutaneous pockets. the implants were recovered from 2 to 246 days. by histological and fine structural parameters all implants remained viable and their morphological character was maintained. selected mice were injected ... | 1989 | 2817436 |
| on the estimation of pulmonary capillary pressure from arterial occlusion. | we have attempted to evaluate some approaches for estimating pulmonary capillary pressure from the transient pressure data obtained from the tip of the swanz-ganz catheter after inflation of the balloon. to this end experiments were carried out in anesthetized dogs in which pulmonary vasoconstriction was induced by infusion of histamine, serotonin, or norepinephrine or by hypoxia. in a group of closed chest dogs, the time course for the fall in the catheter tip pressure toward the wedge pressure ... | 1989 | 2817586 |