| study of high density escherichia coli fermentation for production of porcine somatotropin protein. | recombinant e. coli strains and culture conditions were studied for the fermentation expression of porcine somatotropin (pst) inclusion bodies under the control of a pl promoter. our objective was to achieve high cell density together with a high level of recombinant protein expression. improved fermentation conditions included oxygen enrichment, yeast extract (ye) effect, optimal specific growth to switch on gene expression, and feeding strategies. to maintain a low residual glucose concentrati ... | 1991 | 1809195 |
| [the role of a secondary immunodeficiency in the pathogenesis of different forms of dysentery]. | | 1991 | 1810052 |
| [the treatment of protracted forms of flexner dysentery]. | the clinical efficacy of sodium nucleinate (sn) combined with splenin and quercetin given for lingering flexner's dysentery in a group of 43 patients was compared with that of routine therapy used in a group of the same number of patients. it has been established that the above drug combination favours the elimination of the pathological symptoms of dyspepsia, the arrest of bacterial isolation and normalization of the immune status. provided the effect of the combined drug treatment is insuffici ... | 1991 | 1810053 |
| [humoral and cellular immunity in patients with acute dysentery]. | | 1991 | 1810054 |
| [the functional status of the endocrine pancreas in patients with acute intestinal infections]. | ria was used to measure the content of insulin, c-peptide and glucagon in blood plasma of 84 patients with acute dysentery and food toxicoinfections. the studies were carried out in the acute disease period and before the discharge from hospital. the data obtained indicate that despite the appreciable differences in the disease pathogenesis, the pathophysiological mechanisms influencing pancreatic function are similar in many respects. the changes are characterized by an increase in plasma insul ... | 1991 | 1810055 |
| serotonin immunoreactivity in the intermediate lobe of the rat pituitary. | immunocytochemical staining for serotonin (5-ht) in paraffin-embedded sections of rat pituitary resulted in the localization of reactive nerve fibres and cell bodies in the intermediate lobe. immunostaining was also found in the anterior and posterior lobes. labelled nerve fibres appear to enter the intermediate lobe from the neural lobe through the interlobular spaces. these fibres are relatively scarce and lightly stained. neuroglandular contacts were identified between varicose nerve endings ... | 1991 | 1810536 |
| [antigenic analysis and identification of serotype for an arguable strain of shigella]. | a strain of shigella, 51331, was isolated abroad in 1935, and stored in the center for medical culture collection, beijing. since the serotype of this strain was arguable in china. it was identified as sh. flexneri var. x with antigenic analysis. as agglutinin absorption technic was used in preparing diagnostic factor serum, it appears that the culture of strain 51331 could be agglutinated by shigella antiserum flexner type 3 (sh. flexneri) made in china and england. the present author suggested ... | 1991 | 1814048 |
| synthesis and siderophore activity of albomycin-like peptides derived from n5-acetyl-n5-hydroxy-l-ornithine. | n5-acetyl-n5-hydroxy-l-ornithine (1), the key constituent of several microbial siderophores, has been synthesized in 23% yield overall from n-cbz-l-glutamic acid 1-tert-butyl ester (6) derived from l-glutamic acid. reduction of 6 to 7 and treatment with n-[(trichloroethoxy)carbonyl]-o-benzylhydroxylamine (8), and diethyl azodicarboxylate and triphenylphosphine followed by deprotection produced the protected n5-acetyl-n5-hydroxy-l-ornithine derivatives 11 and 12 in large quantities (10-20 g). fol ... | 1991 | 1825849 |
| synthesis and siderophore and antibacterial activity of n5-acetyl-n5-hydroxy-l-ornithine-derived siderophore-beta-lactam conjugates: iron-transport-mediated drug delivery. | n5-acetyl-n5-hydroxy-l-ornithyl-n5-acetyl-n5-hydroxy-l-ornithyl-n5-acety l- n5-hydroxy-l-ornithine, the functionally instrumental component of the albomycins and ferrichromes, has been incorporated as a "carrier" substructure into both carbacephalosporin and oxamazin type beta-lactam antibiotics. the previously synthesized protected version of this tripeptide (14) was coupled with various beta-lactam analogues 17, 19, 24, and 25 to give protected conjugates 21, 22, 26, and 27. final deprotection ... | 1991 | 1825850 |
| suppression of vascular injury at the arthus reaction site by a complement inhibitor, 5,5',5''-(1,3,6-naphthalene-triyl-tris[sulfonylimino])-tris (1,3-benzene disulfonic acid) hexasodium salt. | a novel polyanionic complement inhibitor 5,5,5''-(1,3,6-naphthalene-triyl-tris[sulfonylimino])-tris(1 ,3-benzene- disulfonic acid) hexasodium salt (compound iib) was tested for its ability to suppress vascular injury at the site of the arthus reaction (ar). in control animals in which ar was evoked without drug treatment, venules at ar sites ranged from normal (arbitrarily defined as stage i) to destroyed (stage v). between these two ends of the spectrum were venules with an accumulation of cell ... | 1991 | 1828755 |
| localization of interleukin-1 receptor antagonist mrna in rat brain. | interleukin-1 (il-1) is an inflammatory peptide hormone, with potent neuroendocrine effects. il-1 stimulates the central nervous system production of corticotropin-releasing hormone (crh), growth hormone (gh), thyroid-stimulating hormone (tsh), and somatostatin, and inhibits the secretion of prolactin and luteinizing hormone (lh). interleukin-1 receptor antagonist (il-1ra) is a novel cytokine, recently purified, characterized, and cloned. il-1ra is a pure endogenous antagonist of il-1:il-1 funct ... | 1991 | 1829036 |
| localization of interleukin-1 receptor antagonist mrna in rat brain. | interleukin-1 (il-1) is an inflammatory peptide hormone, with potent neuroendocrine effects. il-1 stimulates the central nervous system production of corticotropin-releasing hormone (crh), growth hormone (gh), thyroid-stimulating hormone (tsh), and somatostatin, and inhibits the secretion of prolactin and luteinizing hormone (lh). interleukin-1 receptor antagonist (il-1ra) is a novel cytokine, recently purified, characterized, and cloned. il-1ra is a pure endogenous antagonist of il-1:il-1 funct ... | 1991 | 1829036 |
| [the manifestations of the epidemic process in shigellosis and their theoretical interpretation]. | this work, based on the retrospective analysis of shigellosis morbidity among organized groups of adults, as well as the whole population of the city, demonstrates the manifestations of the epidemic process. water supply was common in the city, while water consumption was autonomous. the organized groups of adults did not use the products of the local milk-processing factory. the following facts were established. the dynamics of morbidity in flexner's dysentery showed the change of dominating va ... | 1991 | 1830443 |
| [the structure of the population morbidity with specific intestinal infections in areas with different intensities in the development of the epidemic process and an evaluation of its manifestations]. | the authors analyze the morbidity structure in five enteric infections (typhoid fever, dysentery caused by shigella flexneri and shigella sonnei, hepatitis a, and hepatitis e (non a, non b) with the fecal/oral mechanism of the agent transmission) in three towns of turkmenia and in the town of novomoskovsk, tula province. the incidence of s. sonnei dysentery was found higher in novomoskovsk and that of the rest enteric infections under study in turkmenia. the incidence of typhoid fever and hepati ... | 1991 | 1836925 |
| ascaris in the common bile duct: diagnosis by ultrasound and endoscopic retrograde cholangiopancreatography (ercp) and extraction by balloon catheter. | this is a case report of ascaris worm in the common bile duct in a 61 year old lady. ultrasound diagnosis was initially made based on the presence of linear hyperreflective foci within the common bile duct. ercp confirmed a single worm within the duct. balloon extraction was then successfully carried out following papillotomy. this case illustrates the value of ultrasound in the definitive diagnosis of biliary ascariasis and the therapeutic role of ercp. | 1991 | 1839928 |
| ascaris in the common bile duct: diagnosis by ultrasound and endoscopic retrograde cholangiopancreatography (ercp) and extraction by balloon catheter. | this is a case report of ascaris worm in the common bile duct in a 61 year old lady. ultrasound diagnosis was initially made based on the presence of linear hyperreflective foci within the common bile duct. ercp confirmed a single worm within the duct. balloon extraction was then successfully carried out following papillotomy. this case illustrates the value of ultrasound in the definitive diagnosis of biliary ascariasis and the therapeutic role of ercp. | 1991 | 1839928 |
| genetic analysis of the enterobactin gene cluster in shigella flexneri. | the genes for transport and synthesis of the phenolate siderophore enterobactin are present on the chromosomes of both ent+ and ent- clinical isolates of shigella flexneri. to determine why ent- s. flexneri isolates fail to express a functional enterobactin system, the structure and expression of enterobactin genes were examined. several alterations may be responsible for the inability of s. flexneri to express enterobactin. (i) the mrna levels produced from the entc and fepb genes were not dere ... | 1991 | 1846151 |
| molecular epidemiology of shigella infections: plasmid profiles, serotype correlation, and restriction endonuclease analysis. | plasmid isolation was used to refine the epidemiologic analysis for 168 shigellosis cases in pima county, ariz. plasmids of less than 20 kb were used for comparison of plasmid profiles. plasmid patterns for each species were distinct. a total of 57 of 74 (77%) shigella flexneri strains could be placed into seven plasmid patterns, 70 of 79 (89%) shigella sonnei strains could be placed into seven patterns, 12 shigella boydii strains could be placed into six patterns, and each of 3 shigella dysente ... | 1991 | 1847148 |
| interstitial chromosomal deletion within 4q11-q13 in a human hepatoma cell line. | southern blot analysis revealed the presence of an aberrant albumin gene as well as a normal one in a human hepatoma cell line, huh-7. a genomic sequence carrying this altered gene was isolated and characterized. this clone contains a 3-kbp 3' segment of the albumin gene linked to a non-albumin sequence at intron 11. the non-albumin sequence is assigned to chromosome 4q12-q13 by in situ hybridization. this indicates an interstitial deletion of a chromosomal segment within 4q11-q13 because the al ... | 1991 | 1847664 |
| kinetic analysis of transport and opioid receptor binding of [3h](-)-cyclofoxy in rat brain in vivo: implications for human studies. | [3h]cyclofoxy (cf: 17-cyclopropylmethyl-3,14-dihydroxy-4,5-alpha-epoxy-6-beta-fluoromorp hinan) is an opioid antagonist with affinity to both mu and kappa subtypes that was synthesized for quantitative evaluation of opioid receptor binding in vivo. two sets of experiments in rats were analyzed. the first involved determining the metabolite-corrected blood concentration and tissue distribution of cf in brain 1 to 60 min after i.v. bolus injection. the second involved measuring brain washout for 1 ... | 1991 | 1847702 |
| virulence-associated chromosomal loci of shigella flexneri identified by random tn5 insertion mutagenesis. | shigellae are the causative agents of bacillary dysentery and are capable of invading epithelial cells, multiplying therein and spreading into adjacent cells. to identify genes on the chromosome associated with the virulence phenotype, 9114 independent tn5 insertion mutants were isolated in a virulent strain of shigella flexneri. by using an in vitro assay for intercellular spread or an animal infection model, the serény test, 50 chromosomal tn5 mutants with reduced virulence were identified. th ... | 1991 | 1849606 |
| evidence that the asparagine 322 mutant of the lactose permease transports protons and lactose with a normal stoichiometry and accumulates lactose against a concentration gradient. | the single asparagine 322 mutant of the lactose permease was made by constructing a hybrid plasmid which contained the amino-terminal coding sequence from the wild-type permease gene and the carboxyl-terminal coding sequence from a previously characterized double mutant permease which contained an asparagine residue at position 322. since histidine at position 322 has been postulated to be critically involved with h+ transport and the active accumulation of sugars, the ability of the asn-322 mut ... | 1991 | 1849889 |
| structure and function of the maize spm transposable element. | the spm's primary transcript is alternatively spliced to give at least 4 different transcripts which code for proteins with extensive overlapping domains. two of these proteins, the tnpa and tnpd gene products, are necessary and sufficient to promote dspm transposition in tobacco. moreover, the tnpa gene product is also sufficient to suppress the basal level of expression observed in some dspm-disrupted genes, and probably to activate the expression of inactive elements and of other dspm-disrupt ... | 1991 | 1851643 |
| miniature manipulator with microelectrode for chronic recording of single units from freely-moving animals. | we report a simple recording and stimulating system, a miniature manipulator with a microelectrode (mmm) that can be mounted on animal's skull. the system consists of a miniature manipulator and a microelectrode which are used in one body. several mmms can be implanted simultaneously into the brain as the part of the manipulator is small. with this device, recording of single units from several different cortices is possible from awake and unrestricted animals. | 1991 | 1851979 |
| photoaffinity labeling of rat type i iodothyronine deiodinase. | the photoreactive compound p-nitrophenyl-2-diazo-3,3,3-trifluoropropionate (pal) was coupled to [125i]rt3, t4, or t3 and incubated with liver and kidney microsomes of hypo-, hyper-, or euthyroid rats to identify the type i iodothyronine deiodinase. various substrates or inhibitors of the enzyme, including rt3, t4, t3, 6-n-propylthiouracil (ptu), and iopanoic acid, were used as competitors to establish the specificity of protein labeling. the pal derivatization enhanced the behavior of t4 and t3 ... | 1991 | 1855452 |
| trihexyphenidyl enhances physostigmine prophylaxis against soman poisoning in guinea pigs. | the protective effects of simultaneous and continuous administration of physostigmine and trihexyphenidyl against soman-induced toxicity were studied in guinea pigs. not only did trihexyphenidyl reduce physostigmine-induced toxicity when it was administered continuously to the animals along with physostigmine, the combination afforded greater protection from soman lethality than did either agent administered alone. the combination pretreatment also gave better protection against soman-induced bo ... | 1991 | 1855620 |
| prospective hospital based study on persistent diarrhoea. | a total of 383 children aged less than 5 years suffering from acute watery diarrhoea or dysentery were studied in hospital to determine the rate of persistent diarrhoea. altogether 335 (87.5%) recovered within 13 days. only in 48 (12.5%) did the diarrhoea continue for 14 days or more, and they were considered as having persistent diarrhoea. children aged between 7 and 18 months had a significantly increased incidence of persistent diarrhoea. children suffering from grade ii-iv malnutrition const ... | 1991 | 1855686 |
| the role of c5 and t-cell receptor vb genes in susceptibility to collagen-induced arthritis. | collagen-induced arthritis (cia) is a rodent arthritis model in which immunization with heterologous type ii collagen induces an inflammatory polyarthritis. susceptibility to the disease is mediated by major histocompatibility complex (mhc) genes as well as genes at other loci. previous studies of the swr/j mouse strain, which is resistant to cia despite bearing the susceptible h-2q haplotype, have suggested that this resistance is the result of a deletion of t-cell receptor (tcr) vb gene segmen ... | 1991 | 1855815 |
| isolation of mosquito-pathogenic bacillus sphaericus & b. thuringiensis from the root surface of hydrophytes. | attempts were made to isolate b. sphaericus and b. thuringiensis active against mosquito larvae from the root surface of hydrophytes. out of 139 samples processed, 86 b. sphaericus and 23 b. thuringiensis isolates were obtained. sixty two of the b. sphaericus isolates belonged to the serotype h5a5b, 2 to h6 and 22 isolates did not agglutinate with any of the 6 antisera tested. twenty of the b. thuringiensis isolates belonged to the h14 serotype, 1 each to the h10 and h17 serotype(s) and 1 to an ... | 1991 | 1855819 |
| in vitro effect of phyllanthus amarus on hepatitis b virus. | to evaluate the effects of p. amarus on hepatitis b virus (hbv) antigens and hbv-dna, initial ethanolic extract and subsequent fractions of the plants were prepared. the whole plant material was dried, powdered and extracted with alcohol and subsequently fractionated in hexane, chloroform, butanol and finally in water. all the material were tested for in vitro effects on hbsag, hbeag and hbv-dna in serum samples positive for hbv antigens followed by the screening of respective antigens by elisa. ... | 1991 | 1855821 |
| subcellular fractions of rat organs contain nitroreductases which reduce n-nitrodimethylamine to n-nitrosodimethylamine. | the nasal carcinogen n-nitrodimethylamine was reduced to its n-nitroso analogue n-nitrosodimethylamine in vivo. liver-soluble fraction and brain mitochondria contained enzymes capable of reducing this compound. the activity was only partly inhibited by oxygen; nadph and nadh served as cosubstrates. n-nitromethylamine is also carcinogenic and was reduced by liver cytoplasm to a protein binding species (methyldiazohydroxide?). | 1991 | 1855880 |
| temperature-regulated expression of invasion genes in shigella flexneri is controlled through the transcriptional activation of the virb gene on the large plasmid. | the invasion phenotype of shigellae is subject to thermoregulation that is known to be expressed through activation of some invasion (inv) genes such as ipab, ipac, and ipad encoded by the large virulence plasmid of shigella flexneri. the expression of ipa genes is regulated positively by virf through the activation of virb on the plasmid. to identify the mediator for the thermoregulation of the large plasmid, we have studied the effect of temperature on the transcription of virf and virb genes ... | 1991 | 1857209 |
| glucagon-induced changes in plasma thyroid hormone concentrations in healthy dogs resemble "euthyroid sick syndrome". | we recently demonstrated that glucagon infusion induced a decline in t3 and a rise in rt3 in anesthetized dogs. these changes in t3 and rt3 may be attributed, at least in part, to anesthesia itself, since general anesthesia is known to cause lowering of t3 and an elevation in rt3 during the perioperative period. therefore, to eliminate the contribution, if any, of anesthesia to these changes in t3 and rt3, we assessed plasma glucose, t3 resin uptake (t3ru), t4, free t4, t3 and rt3 concentrations ... | 1991 | 1865078 |
| stress modulates calcium mobilization in immune cells. | both acute and chronic restraint stress modulated mitogen-induced increases in cytoplasmic free-calcium concentrations ([ca2+]i) in mouse spleen cells. dual-color analysis of lymphocyte subpopulations demonstrated that acute (2 hour) restraint stress suppressed mitogen-stimulated increases in [ca2+]i in cd4+ t cells, but enhanced [ca2+]i in cd8+ t cells. chronic restraint stress (2 hours daily for up to 21 days) resulted in a significant suppression of mitogen-stimulated increases in [ca2+]i in ... | 1991 | 1865761 |
| [contacts between the cells in bacterial colonies]. | the interaction of cells in microbial colonies has been studied by electron-microscopic techniques. two types of contacts between cells have been found to exist in the colonies of gram-negative bacteria of the genera escherichia, shigella and salmonella: close cell adhesion due to the fusion of cell-wall outer membranes and the formation of intersections consisting of membranous tubules. at the sites of close adhesion the fusion of cytoplasmic and outer membranes have been found to occur in baye ... | 1991 | 1867039 |
| effect of prior infection with virulent shigella flexneri 2a on the resistance of monkeys to subsequent infection with shigella sonnei. | all virulent shigellae have large plasmids. plasmid-associated genes encode the expression of membrane-associated proteins (map), some of which correlate with the ability to invade susceptible epithelial cells. these map are serologically related in all of the shigella serotypes and evoke an antibody response after infection. to determine whether the map have a significant role in protection, 24 monkeys were infected with virulent shigella flexneri 2a. after recovery, one group (with controls) w ... | 1991 | 1869840 |
| virulence phenotype and genetic characteristics of the t32-istrati shigella flexneri 2a vaccine strain. | the t32-istrati strain, which has been used as an oral attenuated shigella flexneri 2a vaccine, has lost the invasive phenotype due to a spontaneous deletion in the shigella virulence plasmid. this deletion has eliminated three plasmid loci (ipabcda, inva and virg) that are necessary for production of a positive sereny test by shigella species. virulence in the sereny test was reconstituted in the t32-istrati strain by the conjugal transfer of an intact 140 m da virulence plasmid from s. flexner ... | 1991 | 1872021 |
| antigen sharing of salmonella typhi non-flagellar proteins with other salmonellae and some shigellae and escherichia coli. | cathodal moving protein components were identified in agarose gel electrophoresis of the veronal buffer extract of a non-motile strain of s. typhi (8393, colindale). rabbit antiserum was raised against these cationic proteins; it had both agglutinating and precipitating activity. a total of 80 salmonella strains belonging to serogroups a, b, c1, c2, d, e1 and e2 including 26 s. typhi and 10 s. paratyphi a were tested against this antiserum in a slide agglutination test; all strains were agglutin ... | 1991 | 1874550 |
| different patterns of behavior produced by haloperidol, pentobarbital, and dantrolene in tests of unconditioned locomotion and operant responding. | three motor-impairing drugs with different putative mechanisms of action (haloperidol 0.00, 0.075, 0.15, 0.30 mg/kg ip; pentobarbital 0.00, 4.5, 9, 12 mg/kg ip; and dantrolene 0.00, 5, 7.5, 10 mg/kg ip) produced strikingly similar patterns of dose-dependent attenuation in unconditioned locomotor behavior. however, the same drugs and doses produced highly divergent patterns of disruption when tested using different groups of rats in a food-rewarded operant task, which included both response initi ... | 1991 | 1876659 |
| potentials of shigella flexneri y strain tsf21 as a candidate vaccine against shigellosis: safety, immunogenicity and protective efficacy in bonnet monkeys. | a thymine-requiring and temperature-sensitive mutant of shigella flexneri y was tested in bonnet monkeys for safety, immunogenicity and protective efficacy. a dose of 10(11) cells when fed orally mimicked natural infection in having invaded epithelial cells, but was otherwise clinically non-reactogenic. animals immunized with two oral doses, each dose consisting of 1 x 10(11) mutant bacteria, were fully protected when challenged, with respect to the lack of any clinical symptoms or detectable hi ... | 1991 | 1878259 |
| a case of shigellosis caused by shigella dysenteriae type 1 and shigella flexneri type 5b. | shigella dysenteriae type 1 and shigella flexneri type 5b strains were isolated as causative agents of bacterial dysentery in a patient having visited south-east asia. both strains are a rare finding for bulgaria. s. dysenteriae 1 strains have not been isolated since 1962, and there were only single isolates of s. flexneri 5b. the strains were of the same antibiotic resistance patterns. conjugation experiments showed that resistance is determined by transferrable r-plasmids having identical char ... | 1991 | 1880406 |
| [shigella endotoxin protein--its electrophoretic and serological properties]. | the electrophoretic analysis of lipid a-associated protein (lap), obtained from s. sonnei, in polyacrylamide gel in the presence of sodium dodecyl sulfate and urea has revealed the heterogeneity of the preparation; it has found to contain three main components with molecular weights of 43, 38 and 18 kd and some minor components with molecular weights of 49, 45 35, 30, 29, 27, 5, 21 and 14 kd. the electrophoretic mobility of the main protein components in the isolated preparation of lap coincides ... | 1991 | 1882599 |
| [the action of ribosomal preparations on nonspecific resistance to bacterial infection and on early tolerance to endotoxic shock]. | ribosomal preparations from shigella flexneri and shigella sonnei, introduced parenterally into mice, enhance their resistance to infection with the causative agents of typhoid fever and staphylococci. this effect is considerably less pronounced than that produced by the preparation of homologous lipopolysaccharide isolated by boivin's method. after the administration of ribosomes nonspecific resistance to bacterial infective agents lasts for a short time. ribosomal preparations do not enhance t ... | 1991 | 1882607 |
| effects of lactational status on forage intake, digestibility, and particulate passage rate of beef cows supplemented with soybean meal, wheat middlings, and corn and soybean meal. | sixteen mature, lactating (453 kg) and 16 nonlactating (487 kg) hereford and angus x hereford cows were used to determine effects of different dietary supplements and lactational status on forage intake, digestibility, and particulate passage rate. supplement treatments and amounts fed (kg/d) were as follows: control, 0; and equal daily amounts of cp from soybean meal (sbm), 1.36; wheat middlings (wm), 3.41; or a blend of corn and soybean meal (corn-sbm; 22% corn and 76% sbm), 3.41. cows were fe ... | 1991 | 1885374 |
| effects of lactational status on forage intake, digestibility, and particulate passage rate of beef cows supplemented with soybean meal, wheat middlings, and corn and soybean meal. | sixteen mature, lactating (453 kg) and 16 nonlactating (487 kg) hereford and angus x hereford cows were used to determine effects of different dietary supplements and lactational status on forage intake, digestibility, and particulate passage rate. supplement treatments and amounts fed (kg/d) were as follows: control, 0; and equal daily amounts of cp from soybean meal (sbm), 1.36; wheat middlings (wm), 3.41; or a blend of corn and soybean meal (corn-sbm; 22% corn and 76% sbm), 3.41. cows were fe ... | 1991 | 1885374 |
| [use of immunofluorescence method in the diagnosis and study of pathogenesis of acute dysentery]. | | 1991 | 1887317 |
| [pathology of the gastrointestinal tract in patients with reactive arthritis and its possible role in the pathogenesis of the disease]. | | 1991 | 1887410 |
| ompb (osmo-regulation) and icsa (cell-to-cell spread) mutants of shigella flexneri: vaccine candidates and probes to study the pathogenesis of shigellosis. | genetic and molecular data now available on the pathogenic properties of shigella flexneri allow rational design of live attenuated vaccine strains. the genes required at given steps of the infection process can be selectively mutated to impair the bacterium's capacity to interact with intestinal epithelial cells and/or survive within intestinal tissues in general. we have tested two mutations in s. flexneri serotype 5a (m90t) which, alone or in combination, have yielded promising results when e ... | 1991 | 1887672 |
| [the determination in saliva of iga antibodies to shigella ribosomes for the diagnosis of dysentery]. | the levels of antiribosomal antibodies to shigella ribosomes in serum and saliva samples from 38 dysentery patients (15 s. sonnei cases and 23 s. flexneri cases), 14 patients with salmonellosis and 136 healthy adults were determined in elisa with ribosomes from s. sonnei r-mutant used as solid-phase antigen. high levels of "normal" antiribosomal iga, igg and igm antibodies were revealed in the sera of healthy persons while the level of salivary iga antibodies was very low. in dysentery infection ... | 1991 | 1887713 |
| [an immunomorphological study of the lymphoid organs in the experimental administration of virulent and avirulent shigella flexneri 2a strains]. | | 1991 | 1887717 |
| simultaneous infection with multiple serotypes of shigellae in a patient. | we isolated three different serotypes of shigella on admission from a patient with dysentery as well as a shigella-like organism and campylobacter jejuni upon follow-up. the patient produced serum antibodies to all three serotypes of shigellae. | 1991 | 1889185 |
| [changes in virulence of enterobacteriaceae in the presence of sub-inhibitory levels of antibiotics]. | the effect of subinhibitory concentrations of antimicrobial agents such as chloramphenicol, tetracycline, rifampicin and nalidixic acid on various factors of virulence in shigella spp. and enteroinvasive colon bacilli was studied. it was shown that under such conditions changes took place in various events of the interaction with the epithelial cells, i.e. in adhesion, penetration, intracellular multiplication and transfers between contacting cells. the total effect of chloramphenicol resulted i ... | 1991 | 1892427 |
| shigella flexneri infection: a histopathologic study of colonic biopsies in monkeys infected with virulent and attenuated bacterial strains. | macaca fascicularis monkeys were orally infected with live virulent shigella flexneri wild-type strains of either serotype y (s. flexneri sfl1), 2a (s. flexneri m4243) or 1b (s. flexneri sfl27). clinical signs of shigellosis varied from mild watery diarrhea (sfl1) to dysentery (m4243, sfl27), with a fatal outcome in two monkeys (sfl27). colonoscopy showed slight pathologic changes in monkeys infected with sfl1, and pronounced changes in monkeys infected with sfl27. in colonic biopsies the most s ... | 1991 | 1892634 |
| cytogenesis in the monkey retina. | time of cell origin in the retina of the rhesus monkey (macaca mulatta) was studied by plotting the number of heavily radiolabeled nuclei in autoradiograms prepared from 2- to 6-month-old animals, each of which was exposed to a pulse of 3h-thymidine (3h-tdr) on a single embryonic (e) or postnatal (p) day. cell birth in the monkey retina begins just after e27, and approximately 96% of cells are generated by e120. the remaining cells are produced during the last (approximately 45) prenatal days an ... | 1991 | 1894769 |
| effects of interleukin-6, interleukin-2, and tumor necrosis factor alpha on transferrin release from sertoli cells in culture. | recent studies demonstrate that several cytokines are potent modulators of steroid release from the testis. in an attempt to determine whether these agents may influence other types of secreted substances, we used plaque assays to measure the effect of interleukin-6 (il-6), interleukin-2 (il-2), and tumor necrosis factor alpha on transferrin (tf) release from sertoli cells in culture. because sertoli cells from different parts of the tubule respond differently to modulatory factors, we used cult ... | 1991 | 1905226 |
| evidence that inhibin plays a major role in the regulation of follicle-stimulating hormone secretion in the fully adult male rhesus monkey (macaca mulatta). | in the juvenile male rhesus monkey in which an adult-like pattern of endocrine activity in the pituitary-testicular axis is imposed by pulsatile stimulation with exogenous gnrh, administration of inhibin antiserum elicits a marked and selective hypersecretion of fsh. this finding suggests that in the monkey, testicular inhibin plays a major role in the postpubertal regulation of this gonadotropin. the purpose of the present study was to confirm this view more directly. to this end, 10 adult male ... | 1991 | 1905228 |
| susceptibility of pseudomonas aeruginosa and escherichia coli biofilms towards ciprofloxacin: effect of specific growth rate. | methods of cell culture which enable the control of specific growth rate and expression of iron-regulated membrane proteins within gram-negative biofilms were employed for various clinical isolates of pseudomonas aeruginosa taken from the sputum of cystic fibrosis patients and of a laboratory strain of escherichia coli. susceptibility towards ciprofloxacin was assessed as a function of growth-rate for intact biofilms, cells resuspended from the biofilms and also for newly formed daughter cells s ... | 1991 | 1905285 |
| umbilical cord occlusion stimulates breathing independent of blood gases and ph. | the role of umbilical cord occlusion in the initiation of breathing at birth was investigated by use of 16 unanesthetized fetal sheep near full term. artificial ventilation with high-frequency oscillation was used to control fetal arterial blood gas tensions. at baseline, pco2 was maintained at control fetal values and po2 was elevated to between 25 and 50 torr. in the first study on six intact and four vagotomized fetuses, arterial pco2 and po2 were maintained constant during two 30-min periods ... | 1991 | 1905291 |
| measurement of human cerebral monoamine oxidase type b (mao-b) activity with positron emission tomography (pet): a dose ranging study with the reversible inhibitor ro 19-6327. | eight normal subjects (3 females and 5 males) were studied using intravenous l-[11c] deprenyl and positron emission tomography. in a single blind study one subject received tracer alone, one subject received an oral pre-dose of 20 mg of l-deprenyl and 6 subjects received oral pre-doses of 10 to 50 mg of a novel reversible mao-b inhibitor (ro 19-6327). dynamic pet scans beginning 12 h after the oral dose were collected over 90 min and arterial blood was continuously sampled. data analysis was mod ... | 1991 | 1906004 |
| capsaicin inhibits airway hyperresponsiveness but not lipoxygenase activity or eosinophilia after repeated aerosolized antigen in guinea pigs. | to evaluate the role of tachykinins in airway hyperresponsiveness following repeated aerosolized antigen challenge in guinea pigs, we treated 12 guinea pigs with capsaicin (105.6 mg cumulative dose given subcutaneously over 5 days) after sensitization to ovalbumin (oa) and before three repeated oa aerosol challenges per wk for 4 to 5 wk. ten guinea pigs received identical oa sensitization and challenges without capsaicin treatment, and four of eight nonsensitized controls received capsaicin foll ... | 1991 | 1907116 |
| studies on the pathogenicity of listeria monocytogenes. | the characterization of mutants of listeria monocytogenes with reduced virulence properties is described. reduction in the amount of the extracellular protein p60 (encoded by the ipa gene) leads to cell filaments with impaired invasiveness. mutants which cannot synthesize listeriolysin are still invasive but unable to survive within phagocytic cells. one type of listeriolysin-negative mutants is defective in the synthesis of a positive regulatory element prfa which coordinately regulates the lis ... | 1991 | 1908834 |
| intercellular spread of shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin. | the spread of shigella flexneri in a monolayer of infected henle and hela cells was studied by using immunofluorescence and electron microscopy. infected cells produced numerous bacterium-containing membranous protrusions up to 18 microns in length that penetrated adjacent cells and were subsequently phagocytosed. fluorescence staining of actin and vinculin in infected cells with phalloidin and monoclonal antibody to vinculin, respectively, demonstrated that the protrusions containing the bacter ... | 1991 | 1910001 |
| calcium dependence of effects of endothelin on rat mesenteric microvessels. | we investigated the calcium dependence of the effects of endothelin (et) on resistance vessels (less than 300 microns lumen diameter) from the mesenteric vascular bed of the rat, mounted on a wire myograph. et-1 induced a potent sustained contraction with an ed50 of 12 nmol/l. the response to et-3 and big et at the maximum concentrations used (100 nmol/l) was less than 40% of that to et-1, with an estimated ed50 of 45 nmol/l. relaxation of the et-1-induced contraction was slow, and resulted in a ... | 1991 | 1913326 |
| biosynthesis of isochorismate in klebsiella pneumoniae: origin of o-2. | the shikimate metabolites are key precursors to a large number of natural products, including aromatic amino acids. chorismic acid is an important branch point in the biosynthetic pathway to aromatic amino acids. chorismic acid is also unique among natural products since it is the only compound known to undergo an enzymatic claisen rearrangement. a metabolite of chorismic acid, isochorismic acid, first observed in aerobacter aerogenes differs in its chemical structure by the location of the hydr ... | 1991 | 1913340 |
| relationship of fever magnitude to rate of serious bacterial infections in infants aged 4-8 weeks. | we correlated the height of fever with underlying infectious etiology in 683 consecutive febrile infants aged four to eight weeks who received outpatient evaluation for sepsis during a five-year period. the relative number of infants with fever was inversely proportional to fever height, as 51% had a temperature 38.1-38.9 degrees c, 45% had a temperature 39-39.9 degrees c, and 4% had a temperature greater than or equal to 40 degrees c [hyperpyrexia]. there were 34 cases of serious bacterial infe ... | 1991 | 1914347 |
| screening of children for enteric bacterial pathogens in the outborn neonatal ward in lagos, nigeria. | babies, on admission into a neonatal ward at the lagos university teaching hospital, had their rectal swab specimens examined bacteriologically and screened for enteric bacterial pathogens over a one-year-period at two-week intervals. it was found that on the average there were 3 (9.68%) enteric bacterial pathogens out of an average of 31 admissions at each screening period. the enteric bacterial pathogens isolated included: non-typhoid salmonellae, which accounted for 55 (80.88%) isolates out o ... | 1991 | 1915794 |
| 140-mda plasmids of escherichia coli and shigella flexneri 5 do not influence phagocytosis. | enteroinvasive escherichia coli (eiec) and shigella flexneri possessing a 140-megadalton (mda) plasmid are capable of invading intestinal epithelial cells and causing dysentery. to determine if this plasmid affected phagocytosis of the organisms by leukocytes, we studied the in vitro phagocytosis of isogenic pairs of eiec and s. flexneri 5 which differed only in the presence or absence of the 140-mda plasmid. in addition five eiec strains containing 140-mda plasmids as well as one non-enteroinva ... | 1991 | 1921856 |
| nucleotide sequence and phylogeny of a chloramphenicol acetyltransferase encoded by the plasmid pscs7 from staphylococcus aureus. | the nucleotide sequence of the chloramphenicol acetyltransferase gene (cat) and its regulatory region, encoded by the plasmid pscs7 from staphylococcus aureus, was determined. the structural cat gene encoded a protein of 209 amino acids, which represented one monomer of the enzyme chloramphenicol acetyltransferase (cat). comparisons between the amino acid sequences of the pscs7-encoded cat from s. aureus and the previously sequenced cat variants from s. aureus, staphylococcus intermedius, staphy ... | 1991 | 1929326 |
| reactive modifications of the autonomous time structure in the human organism. | the spectrum of biological rhythms exhibits characteristic principles of biological time structure which also rule the functional behaviour. with increasing period lengths the rhythms become increasingly complex. in the long-wave section the rhythmic functions find their corresponding cycles in the environment, whereas the shorter waves represent only endogenous autonomous rhythms, which maintain an internal time order by means of frequency- and phase-coordination. under resting conditions and i ... | 1991 | 1932773 |
| effect of a nonpeptide angiotensin ii receptor antagonist, dup 753, on angiotensin-related water intake in rats. | the effect of peripheral administration of the nonpeptide angiotensin ii-1 (aii) receptor blocker, dup 753, on the dipsogenic responses to peripherally administered angiotensins i, ii, and iii was tested. in all cases, dup 753 significantly inhibited the drinking response, whether administered 15 or 45 minutes prior to administration of the dipsogen. these results suggest that the drinking responses to angiotensins i, ii, and iii are mediated by aii-1 receptors. they also suggest that either aii ... | 1991 | 1933441 |
| a new sensitive method for determining endotoxin in whole blood. | we developed a microplate method for determining endotoxin in whole blood with endospecy, an endotoxin-specific chromogenic limulus test reagent. the factors in blood that would interfere with the test were successfully removed by exposing samples to 0.66 mol/l hno3 containing 0.25% triton x-100. recoveries of various endotoxins spiked into whole blood of humans and experimental animals were almost complete, and were not enhanced by sample dilution. normal endotoxin concentration in human whole ... | 1991 | 1934509 |
| development of antisera to detect virulent yersinia enterocolitica, shigella and escherichia coli enteroinvasive. | | 1991 | 1935085 |
| molecular epidemiology of plasmid patterns in shigella flexneri types 1-6. | a total of 123 drug-resistant and drug-sensitive shigella flexneri types 1-6, and their escherichia coli k12 transconjugants were used for plasmid profile analysis by agarose gel electrophoresis. resistance factors (r-factors) were further characterized by incompatibility testing. the overall distribution of small plasmids in s. flexneri showed that a cryptic plasmid of about 4.6 kb was found in all serotypes, and a plasmid of about 4.2 kb was found in serotypes 1-4. shigella flexneri types 2, 4 ... | 1991 | 1936154 |
| small-animal model to measure efficacy and immunogenicity of shigella vaccine strains. | the development of a small-animal model to test the protective efficacy and immunogenicity of a vaccine strain against shigellosis would greatly facilitate the evaluation of potential vaccine candidates. in guinea pigs, the ability of shigellae to invade and multiply within the corneal epithelium, causing keratoconjunctivitis, closely mimics the invasion process in the intestinal epithelium (b. sereny, acta microbiol. acad. sci. hung. 4:367-376, 1957). the serum response of animals recovering fr ... | 1991 | 1937767 |
| inhibition of chemotaxis of neutrophil leukocytes to interleukin-8 by endotoxins of various bacteria. | the effects of endotoxins from various bacteria (escherichia coli, klebsiella pneumoniae, vibrio cholerae, shigella flexneri, salmonella typhosa, and pseudomonas aeruginosa) on chemotaxis of neutrophil leukocytes to formyl peptide and interleukin-8 were tested in an improved chemotaxis assay involving a "sparse-pore" polycarbonate (nuclepore) membrane in a boyden-type chamber. the possible chemotactic activity of the endotoxins themselves were tested by the same technique. in addition, the effec ... | 1991 | 1937782 |
| antibody responses in the lungs of mice following oral immunization with salmonella typhimurium aroa and invasive escherichia coli strains expressing the filamentous hemagglutinin of bordetella pertussis. | the filamentous hemagglutinin (fha) of bordetella pertussis was expressed in the attenuated aroa mutant of salmonella typhimurium, sl3261, and in a strain of escherichia coli harboring shigella flexneri plasmid pwr110, which encodes bacterial invasiveness for epithelial cells. expression of fha in these strains did not interfere with their ability to invade henle cells. immunoglobulins a and g specific for fha were detected in lung washes of mice following oral immunization with the live recombi ... | 1991 | 1937797 |
| preparation, characterization, and immunogenicity of conjugates composed of the o-specific polysaccharide of shigella dysenteriae type 1 (shiga's bacillus) bound to tetanus toxoid. | the background for developing conjugate vaccines for shigellosis composed of the o-specific polysaccharide (o-sp) bound to a protein is described elsewhere (c. y. chu, r. schneerson, and j. b. robbins, submitted for publication). briefly, there is direct evidence for type (lipopolysaccharide [lps])-specific protection after infection with the wild type or with attenuated strains of shigellae. prospective studies of israeli armed forces recruits show a correlation between preexisting serum immuno ... | 1991 | 1937803 |
| treatment of shigellosis. | | 1991 | 1941398 |
| a new form of autosomal dominant arthrogryposis. | we report a man and his son with congenital limb contractures, limitation of ocular movements, and an electroretinal abnormality. they appear to have an autosomal dominant form of arthrogryposis, distinguishable from other previously classified forms of this disorder. | 1991 | 1941966 |
| [creatinine clearance as the foundation for clinical determination of kidney function in swine]. | comparative examinations of renal function using inulin- and endogenous creatinine-clearance tests in 5 pigs showed nearly identical values of glomerular filtration rate (gfr). based on this relation 79 healthy pigs, weighting 2 kg to 230 kg were investigated using the creatinine-clearance test for determination of normal values of gfr and urine flow rate (vu) as well as renal excretion (e), renal clearance (clr) and fractional excretion (fe) of urea, sodium, potassium, calcium, phosphorus, gluc ... | 1991 | 1948984 |
| [the adhesion of pathogenic microflora on carbon sorbents]. | the adsorbing activity of granulated carbonic sorbents skn and kau, as well as their oxidated forms, containing protogenic carboxylic and phenolic groups with respect to shigella flexneri, salmonella typhimurium, escherichia coli, streptococcus aureus and pseudomonas aeruginosa pathogenic strains has been studied. as shown in this study, the process of interaction between microorganisms and carbonic sorbents has two stages. at the first stage the main role is played by long-distance electrostati ... | 1991 | 1950254 |
| [the dynamics of the formation of an infection focus in the intestines]. | the problem of the stability of the development of shigella population in the intestine, depending on the dose of the infective agent, is analyzed. the agent is reproduced in the intestinal mucosa in short cycles, starting with adhesion and followed by penetration, intracellular multiplication and, finally, emission of free bacteria and hulled tops of villi with shigella intracellular microcolonies. this form of emission maintains high concentration of bacteria and ensures, in case of the adhesi ... | 1991 | 1950279 |
| [the protective properties of the endotoxin protein]. | the isolation and properties of endotoxin protein, or lipid a-associated protein (lap), from shigella sonnei were described earlier (zh. mikrobiol. epidemiol. immunobiol., 1991, no. 4, pp. 11-17, and no. 7). in this report the data on its protective activity are presented. in experiments on mice one nanogram of lap injected i. v. protected 50% of the animals against i. p. challenge with 40 ld50 of virulent s. sonnei. guinea pigs injected s. c. with 10 micrograms of lap were protected against loc ... | 1991 | 1950285 |
| ototoxic effect of potassium canrenoate on the guinea pig cochlea. | potassium canrenoate (pc) is a diuretic with antialdosterone action, reducing the reabsorption of sodium in the efferent renal tubules. this drug has been reported to damage only the marginal cells of the stria vascularis in the cochlea. the perilymphatic space of the guinea pig cochlea was perfused with an artificial perilymph containing 5 x 10(-3) m potassium canrenoate. following the onset of perfusion, the endocochlear dc potential (ep) gradually declined to around 10 mv but did not become n ... | 1991 | 1950534 |
| jaw protruder muscles and condylar cartilage growth in the rat. | many studies have explored the role of the protrusive musculature in promoting growth at the condylar cartilage and the overall lengthening of the lower jaw, with emphasis on the lateral pterygoid muscle (lpm). the largely anteroposterior orientation of the superficial part of the masseter muscle (sm) in the rat suggests that it may also function as a protruder of the lower jaw. accordingly, it is possible that the action of the sm may play a part in the regulation of growth of the condylar cart ... | 1991 | 1951196 |
| epidemiologic patterns of acute diarrhea and endemic shigella infections in children in a poor periurban setting in santiago, chile. | to prepare a field site for evaluating preventive interventions against endemic shigellosis, the authors followed prospectively a cohort of 360 children (90 each of children aged 0-11, 12-23, 24-35, and 36-47 months) in santa julia, a low socioeconomic area in santiago, chile, from november 1986 through april 1989 with twice weekly household visits for diarrheal disease; infants replaced children who reached 60 months of age. coprocultures on 2 consecutive days from children with diarrhea and fr ... | 1991 | 1951266 |
| static muscle contraction reflexly increases adrenal sympathetic nerve activity in rats. | little is known about the mechanisms responsible for activation of sympathoadrenal function during exercise. we hypothesized that sympathoadrenal discharge is activated at the onset of exercise by a reflex arising in the contracting muscle. adrenal sympathetic nerve activity (sna) was recorded during 1 min stimulation of the tibial nerve at two times motor threshold, before and during neuromuscular blockade, in 12 alpha-chloralose-anesthetized rats. static muscle contractions, induced by stimula ... | 1991 | 1951780 |
| dogs as sentinels for lyme disease in massachusetts. | an investigation of the relationship between incident human cases of lyme disease and seroprevalence of antibodies to b. burgdorferi in dogs was undertaken in order to determine whether dogs might serve as sentinels for lyme disease. | 1991 | 1951802 |
| pathogenicity and antigenic components of excysted giardia lamblia isolated from patients in riyadh, saudi arabia. | giardia lamblia cysts were isolated from patients in riyadh, saudi arabia. cysts and trophozoites (from axenically excysted cysts) were given orally by gavage to mice to establish the pathogenicity of the riyadh isolate. there was no effect of varying the dose of administered parasite on parasite excretion or morbidity. a typical pathologic pattern of giardiasis was demonstrated by histologic methods and electron microscopy. antigenic components of the riyadh isolate were compared with the portl ... | 1991 | 1951852 |
| growth of dengue type 2 virus isolates in human peripheral blood leukocytes correlates with severe and mild dengue disease. | we tested three dengue type 2 (den-2) isolates from children with clinically apparent but mild secondary dengue infections, and 10 isolates from children with moderately severe dengue hemorrhagic fever, and noted significant growth differences in peripheral blood leukocytes, but not in c6/36 cells. we also observed cytopathic effects in c6/36 cells that correlated with disease severity. these preliminary observations suggest the possibility that viral factors, whether surface antigens, attachmen ... | 1991 | 1951875 |
| [cytological examination of urethral and endocervical smears in the diagnosis of infections]. | cytologic studies of smears from 130 women suffering from various diseases, including 61 pregnant patients with gestoses, have shown structural changes of the cells in the smears from the endocervix and urethra, that permitted the diagnosis of mycoplasma infection in 43.8% of patients, chlamydia infection, 52.3%, and herpes simplex in 72.3% of patients; in 63.9% of patients these infections were combined. cytologic studies may be useful in the tentative diagnosis of urogenital infections, includ ... | 1991 | 1951977 |
| [detection of the determinants of tetracycline a, b and c resistance in shigella and salmonella using dna probes]. | genetic determinants of tetracycline resistance of classes a, b and c were detected with dna probes labeled with radioactive phosphorus (32p) in shigella (44 strains) and salmonella (50 strains). it was shown that in the shigella strains the frequency of the tet a gene amounted to 66 per cent, the frequency of the tet b gene was equal to 84 per cent, the frequency of their combination was equal to 50 per cent and the frequency of the tet c gene was nil. in the salmonella strains the frequency of ... | 1991 | 1953181 |
| causes of breast cancer misdiagnosis at physical examination. | 2740 consecutive breast cancers undergoing physical examination were reviewed. ninety-two subclinical cancers detected at mammography were excluded from further evaluation and the study focused on palpable false benign cancers. the sensitivity of physical examination varied according to t category (tis = 0.48, t1 = 0.70, t2 = 0.90, t3 = 0.89, t4 = 0.93), age (20-29 = 0.77, 30-39 = 0.58, 40-49 = 0.75, 50-59 = 0.84, 60-69 = 0.90, greater than 69 = 0.94) and operator (range 0.69-0.89), a significan ... | 1991 | 1956468 |
| the prophenoloxidase system and in vitro interaction of trypanosoma rangeli with rhodnius prolixus and triatoma infestans haemolymph. | the presence of the prophenoloxidase (propo) system in the haemolymph of rhodnius prolixus and triatoma infestans and the role played by trypanosoma rangeli in the in vitro activation of propo were tested. both r. prolixus and t. infestans whole blood preparations showed a very active propo system. the propo cascade of the two insect species were differentially activated by microbial-derived extracts: laminarin was a better activator of t. infestans haemolymph than of r. prolixus blood, and lipo ... | 1991 | 1956701 |
| differences in blood group b-specific mucinase activity between virulent and avirulent shigella flexneri 2a strains. | based on our previous findings we postulate that the production of blood group b-degrading mucinase by shigella flexneri 2a is related to virulence. the virulent s. flexneri 2a strain m4243 produced a blood group b mucinase which decreased the blood group b reactivity of germ-free mouse mucins by a factor of 16 and the b reactivity of human saliva by a factor of 32. avirulent s. flexneri 2a b-1, serologically similar, but not genetically identical to the m4243 strain, failed to degrade the blood ... | 1991 | 1961109 |
| distribution of insertion sequence is200 in salmonella and shigella. | two dna probes for the detection of insertion sequence is200 by either southern blotting or colony hybridization were constructed. one of the probes is a 300 bp ecori-hindiii fragment of is200 cloned onto pbluescript ks(+); the other is a tail-to-tail dimer of the same fragment cloned onto puc19. a survey of the presence of is200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of salmonella spp. examined contained one or more copies of insertion se ... | 1990 | 1964174 |
| h(+)-stimulated release of prostaglandin e2 and cyclic adenosine 3',5'-monophosphoric acid and their relationship to bone resorption in neonatal mouse calvaria cultures. | the addition of protons to the medium of neonatal mouse calvaria cultures stimulated bone resorption and release of calcium into the medium. in addition, added protons significantly increased the release of prostaglandin e2 (pge2) and cyclic adenosine 3',5'-monophosphoric acid (camp) from the bones. indomethacin significantly inhibited the release of calcium, pge2 and camp from proton-treated cultures. the positive control, parathyroid hormone (pth)-treated cultures, also gave rise to bone resor ... | 1990 | 1964815 |
| immunoglobulin profiles and antibacterial antibody levels in 50 patients a long time after posttraumatic splenectomy. | serum immunoglobulin and antibacterial antibody concentrations have been determined in 50 patients 2 to 20 years after posttraumatic splenectomy. the serum levels of igg, iga and igm were found to be significantly greater in the group of posttraumatic splenectomy patients than in that of the healthy controls. the serum ige concentrations did not differ statistically from that of the controls. the serum level of natural antibodies against intestinal bacteria were found to be elevated (shigella 1b ... | 1990 | 1966943 |
| close relationships between neopterin and beta-2-microglobulin levels in intravenous drug abusers. | serum from 36 intravenous drug abusers without acquired immunodeficiency syndrome (aids) or aids-related complex were tested for concentrations of neopterin and beta 2-microglobulin. the seroprevalence of human immunodeficiency virus (hiv) antibody in this group was 50%. previous studies of this group showed that the hiv antibody positive patients had significant increases in hla-dr expression on peripheral blood lymphocytes and increases in serum soluble cd8 antigen. both neopterin and beta 2-m ... | 1990 | 1976599 |
| competitive inhibition of magnesium-induced [3h]n-(1-[thienyl] cyclohexyl)piperidine binding by arcaine: evidence for a shared spermidine-magnesium binding site. | the polyamine competitive antagonist arcaine (1,4-diguanidino-butane) produced complete inhibition of basal [3h]n-(1-[thienyl] cyclohexyl)piperidine ([3h]tcp) binding, with an ic50 value of 4.52 +/- 0.93 microm. arcaine (5 and 10 microm) produced a decrease in the affinity without a significant change in the receptor density of [3h]tcp binding under equilibrium conditions. in addition, arcaine did not alter either n-methyl-d-aspartate-specific [3h] glutamate or strychnine-insensitive [3h]glycine ... | 1990 | 1978243 |