| isolation and characterization of acetylornithine delta-transaminase of wild-type escherichia coli w. comparison with arginine-inducible acetylornithine delta-transaminase. | | 1979 | 112925 |
| transformation of pseudomonas aeruginosa strain pao with bacteriophage and plasmid dna. | a procedure has been developed which allows transformation of p. aeruginosa strain pao with plasmid and bacteriophage dna at a frequency of 10(-6) per recipient cell. the method is similar in outline to that developed for escherichia coli. it involves growing the recipient cells to 3-5 x 10(8) per ml in nutrient broth, washing the cells with 0.1 m mgcl2, resuspending in 0.175 m cacl2 for 20 min, exposing to dna for 1 h and then heat pulsing at 42 degrees c for 1 min. some plasmid markers are exp ... | 1978 | 112956 |
| the effect of phenazine methosulfate-ascorbate on bacterial active transport and adenosine triphosphate formation: inhibition of pseudomonas aeruginosa and stimulation of escherichia coli. | the artificial electron-donor system, phenazine methosulfate (pms) ascorbate, inhibited active transport of glucose by pseudomonas aeruginosa irrespective of whether the incubation systems were in air, flushed with oxygen, or gassed with nitrogen under anaerobic denitrifying conditions. active transport of glucose by p. aeruginosa was also inhibited by reduced 5-n-methyl-phenazonium-3-sulfonate, a membrane-impermeable electron donor. pms-ascorbate caused rapid depletion of intracellular adenosin ... | 1979 | 113071 |
| [in vitro inhibition of trna methyltransferases by queen substance, a pheromone of queen honeybees]. | the queen substance 1, a pheromone of the queen honeybee apis mellifica is an in vitro inhibitor of e. coli b trna methylations. this activity is not specific of the methylase source, as inhibitions have been observed with preparations from queen honeybee ovaries, rat liver or a mouse plasmocytoma 1-adenine methylase. these results, together with preceding ones concerning t, t-farnesyl-acetone 3, are discussed. | 1979 | 113116 |
| uracil incorporation into nascent dna of bacillus subtilis and escherichia coli. | | 1979 | 113168 |
| in vitro efficacy of bay k 4999, a new ureido-penicillin, in combination with aminoglycosides against pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae and proteus strains. | the in vitro effects of bay k 4999 in combination with gentamicin, tobramycin, amikacin sisomicin and netilmicin in bacteriostatic (mic) and bactericidal (mbc) concentrations were compared using the checkerboard dilution technique against 20 different strains of pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae and indole-positive-negative proteus species. on average 63% of bay k 4999-aminoglycoside (ag) combinations inhibited pseudomonas, proteus and klebsiella strains additively ... | 1979 | 113178 |
| a comparison of the in vitro activity of tobramycin and gentamicin against 6,042 clinical isolates. | 6,042 strains of staphylococcus aureus, streptococcus faecalis, proteus mirabilis, escherichia coli, klebsiella sp. and pseudomonas sp. were tested against tobramycin and gentamicin. among all species studies, except pseudomonas sp, gentamicin turned out to be slightly superior to tobramycin. | 1979 | 113179 |
| [expression of deo-operon escherichia coli k-12 genes in the makeup of hybrid plasmid rp4::mu-deo in pseudomonas trifolii and pseudomonas putida]. | | 1979 | 113191 |
| positive control of ilvc expression in escherichia coli k-12; identification and mapping of regulatory gene ilvy. | the construction of a plasmid carrying the ilvc::lacz fusion is described. this plasmid provides a convenient source of template deoxyribonucleic acid for use in an in vitro protein-synthesizing system. we screened strains deleted in regions of the ilv cluster for their ability to support ilvc-dependent beta-galactosidase synthesis. the fact that two deletions prevented beta-galactosidase production indicated that ilv-c expression is under positive control. by use of plasmids carrying the positi ... | 1979 | 113381 |
| regulation of expression of the flagellin gene (hag) in escherichia coli k-12: analysis of hag-lac gene fusions. | previous studies have defined 28 genes necessary for the synthesis of the flagellar apparatus of escherichia coli k-12. this study analyzed the influence of the flagellar genes on the expression of the hag gene (structural gene for flagellin). to this end, a hag::mu d(apr lac) mutant which had the lac genes fused to the promoter of the hag gene was constructed. this allowed the measurement of hag gene expression by detection of beta-galactosidase activity. the following observations were made. ( ... | 1979 | 113385 |
| transposable plasmid deoxyribonucleic acid sequence in pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents. | a 9.1 x 10(6)-dalton transposable deoxyribonucleic acid sequence resides within pseudomonas aeruginosa plasmid r1033 and mediates resistance to gentamicin, streptomycin, sulfamethoxazole, chloramphenicol, and mercuric chloride. transposability was demonstrated in escherichia coli when this sequence, designated tn1696, excised from r1033 and integrated into plasmid pmb8. excision and insertion of tn1696 occurred independently of the host rec phenotype and may involve the 140-base pair, inverted d ... | 1979 | 113388 |
| use of gene fusions to determine a partial signal sequence of alkaline phosphatase. | we have isolated strains of escherichia coli in which an amino-terminal portion of the cytoplasmic enzyme beta-galactosidase is replaced by an amino-terminal portion of the periplasmic enzyme alkaline phosphatase. the synthesis of these hybrid proteins is regulated by inorganic phosphate and they are located in the cytoplasm. one of these proteins was purified, and 14 amino acids of the amino-terminal sequence were determined. the first five amino acids, met-lys-gln-ser-thr, appear to represent ... | 1979 | 113391 |
| purification of bacillus subtilis rna polymerase with heparin-agarose. in vitro transcription of phi 29 dna. | we have devised a new procedure for the purification of highly active preparations of bacillus subtilis rna polymerase holoenzyme. a column of heparin-agarose a-15m is used to rapidly and quantitatively adsorb rna polymerase from the initial crude extract fraction. this affinity procedure obviates the necessity of including nucleic acid precipitation or partitioning steps and allows for rapid separation of rna polymerase from proteolytic activity. the enzyme is further purified by preparative gl ... | 1979 | 113409 |
| fusion of the lac genes to the proximal promoters of the deo operon of escherichia coli k12. | | 1979 | 113489 |
| the nature of transcription selectivity of bacteriophage spo1-modified rna polymerase. | escherichia coli and bacillus subtilis rna polymerase have almost identical transcription specificities on bacteriophage spo1 dna when assayed in a coupled transcription-translation cell free system. spo1-modified b. subtilis rna polymerase has altered transcription specificity. it is shown that rifampicin-inhibited e. coli rna polymerase can completely block transcription of spo1 dna by rifampicin resistant b. subtilis enzyme, whereas it has no effect on transcription by spo1-modified b. subtil ... | 1979 | 113644 |
| different specific activities of the monomeric and oligomeric forms of plasmid dna in transformation of b. subtilis and e. coli. | (1) the low residual transforming activity in preparations of monomeric, supercoiled, circular (ccc) forms of the plasmids pc194 and phv14 could be attributed to the presence in such isolates of a small number of contaminating multimeric molecules. (2) e. coli derived preparations of phv14, as in vitro recombinant plasmid capable of replication in both e. coli and b. subtilis, contain oligomeric forms of plasmid dna in addition to the prevalent monomeric ccc form. the specific transforming activ ... | 1979 | 113646 |
| sequence analysis of cloned cdna encoding part of an immunoglobulin heavy chain. | the recombinant plasmid ph21-1 consists of mouse-derived complementary dna (cdna) in the e. coli plasmid pmb9. the mouse insertion has been completely sequenced, and encodes the ch3 domain and half the ch2 domain of the immunoglobulin gamma1 heavy chain. the predicted amino acid sequence differs at several positions from that previously published for this protein. the pattern of codon usage resembles that in some other eukaryotic messenger rnas. a computer program has been used to predict the op ... | 1979 | 113776 |
| fluorescent anti-colonic and e. coli antibodies in ulcerative colitis. | with the indirect immunofluorescence technique using sections of colon from guenon and rat, sera from patients with ulcerative colitis yielded positive staining of goblet cells. eight sera, thus defined positive, two negative, and five from hospital controls, were applied to agar preparations of 12 different e. coli strains, normally found in human bacterial diseases and e. coli 0 14. with e. coli 0 group antigens, 2, 8 and 14 positive fluorescence reactions were regularly found in sera where po ... | 1978 | 113868 |
| [comparison of bactericidal effects of four aminoglycoside antibiotics: amikacin, gentamicin, kanamycin and tobramycin (author's transl)]. | the authors have studied on 20 bacterial strains (5 pseudomonas aeruginosa, 5 escherichia coli, 5 klebsiella and 5 serratia) the bactericidal kinetics of 4 aminoglycoside antibiotics: amikacin, gentamicin, kanamycin and tobramycin. the antibiotic concentrations used for this work were 1.5 times the mic for each strain previously measured in a liquid medium. the action of the aminoglycoside antibiotics shows three phases. the third phase describes a part of less susceptible bacterial population. ... | 1979 | 114083 |
| transfer of r factors to and between genetically marked sublines of rhizobium japonicum. | plasmids r1822 and prd1 of the p-1 incompatibility group, for which rhizobium japonicum had not previously been shown to serve as host, were introduced into a strain of r. japonicum. acquisition of r68 and r68.45 plasmids by this rhizobium was equivocal. transfer of r1822 from pseudomonas aeruginosa and of prd1 from escherichia coli to r. japonicum was unambiguous, because the exconjugants subsequently cotransferred the three r-factor resistance determinants (kanamycin, tetracycline, and penicil ... | 1979 | 114109 |
| escherichia coli k-12 glycogen synthase: ability to use udpglucose and adp glucose as glucosyl donors in the absence of added primer. | | 1979 | 114117 |
| effect of growth conditions on the synthesis of nitrate reductase components in chlorate resistant mutants of escherichia coli k 12. | | 1979 | 114178 |
| studies of ciliated epithelia of the human genital tract. 3: mucociliary wave activity in organ cultures of human fallopian tubes challenged with neisseria gonorrhoeae and gonococcal endotoxin. | quantative determinations of the mucociliary activity of human fallopian tube epithelium maintained as organ cultures were performed using a light beam reflex method. in non-infected organ cultures the mucociliary wave (mcw) frequency slowly decreased during the first 54 hours of culture maintenance. in organ cultures experimentally infected with fresh isolates of neisseria gonorrhoeae producing t1/t2 colonies the mcw frequency either decreased to subnormal values or completely ceased whereas in ... | 1979 | 114195 |
| nuclear magnetic resonance studies of the binding of trimethoprim to dihydrofolate reductase. | the resonances of the aromatic protons of trimethoprim [2,4-diamino-5-(3',4',5'-trimethoxybenzyl)pyrimidine] in its complexes with dihydrofolate reductases from lactobacillus casei and escherichia coli cannot be directly observed. their chemical shifts have been determined by transfer of saturation experiments and by difference spectroscopy using [2',6'-2h2]trimethoprim. the complex of 2,4-diamino-5-(3',4'-dimethoxy-5'-bromobenzyl)pyrimidine with the l. casei enzyme has also been examined. at ro ... | 1979 | 114205 |
| interaction of divalent cations with beta-galactosidase (escherichia coli). | although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only mg2+ and mn2+ actually did activate. the apparent activation by the other divalent metals was shown to be due to mg2+ impurities. calcium did not activate, but experiments suggested that it did bind. other divalent metals which were studied failed to bind. the dissociation constants for mg2+ and mn2+ were 2.8 x 10(-7) and 1.1 x 10(-8) m, respectively, and in each case one ion bound per mo ... | 1979 | 114210 |
| purification of the messenger ribonucleic acid for the lipoprotein of the escherichia coli outer membrane. | the mrna for the lipoprotein of the escherichia coli outer membrane has been purified to 85% homogeneity. the purification procedure involved phenol extraction, nacl extraction, gel filtration on sephadex g-100 and sephadex g-200, and reversed-phase column chromatography on rpc-5. the purity of the final product was estimated to be 85% by analysis of the ribonuclease t1 fingerprint of the mrna. the purified mrna was able to direct the synthesis of cross-reactive material with antilipoprotein ser ... | 1979 | 114211 |
| the ineffectiveness of analogs of d-galactal as competitive inhibitors of, and substrates for, beta-d-galactosidase from escherichia coli. | 2,6-anhydro-3-deoxy-aldehydo-d-lyxo-hept-2-enose (7) and 2,6-anhydro-3-deoxy-d-lyxo-hept-2-enitol (8) were synthesized as half-chair analogs of d-galactal (1). as 1 is a strong inhibitor of, as well as a substrate for, beta-d-galactosidase from escherichia coli, the same properties were expected for 7 and 8; however, both were ineffective. this result, together with those of other authors, allows speculative conclusions on the tight binding of 1 to the enzyme only, when water or an alcohol is bo ... | 1979 | 114299 |
| comparative studies of penicillin-binding proteins in pseudomonas aeruginosa and escherichia coli. | penicillin-binding proteins in pseudomonas aeruginosa were compared with those of escherichia coli. these in p. aeruginosa were found exclusively in the cytoplasmic membrane fraction (fraction soluble in sodium n-lauroyl sarcosinate). sodium dodecyl sulfate/acrylamide gel electrophoresis of the proteins bound to [14c]penicillin g resulted in the separation of six major bands and several minor bands. the proteins in these bands are referred to as proteins 1a, 1b, 2, 3, 4 and 5 in order of increas ... | 1979 | 114388 |
| acidic ribosomal proteins from eukaryotic cells. effect on ribosomal functions. | precipitation of saccharomyces cerevisiae ribosomes by ethanol under experimental conditions that do not release the ribosomal proteins can affect the activity of the particles. in the presence of 0.4 m nh4cl and 50% ethanol only the most acidic proteins from yeast and rat liver ribosomes are released. at 1 m nh4cl two more non-acidic proteins are lost from the ribosomes. the release of the acidic proteins causes a small inactivation of the polymerizing activity of the particles, additional to t ... | 1979 | 114391 |
| fluorescent derivatives of yeast trnaphe. | the preparation of four fluorescent derivatives of trnaphe (yeast) and their characterization by chemical, spectroscopic, and biochemical methods is described. the derivatives are prepared by replacing wybutine (position 37 in the anticodon loop) or nabh4-reduced dihydrouracil (positions 16/17 in the hu loop) with ethidium or proflavine; they are isolated by reversed-phase chromatography (rpc-5). all trnaphe-dye derivatives are aminoacylated by yeast phenylalanyl-trna synthetase to at least 80% ... | 1979 | 114393 |
| action of bacterial endotoxin and lipid a on mitochondrial enzyme activities of cells in culture and subcellular fractions. | escherichia coli o127:b8 lipopolysaccharide (lps), prepared by the westphal procedure, caused a marked decrease in the activities of mitochondrial malate dehydrogenase, succinate dehydrogenase, and adenylate kinase in african green monkey kidney (vero) cells and primary cultures of mouse liver cells within 2 h after exposure to 10 micrograms of lps/ml of culture medium. these three enzyme activities leaked into the supernatant fraction, and cytochrome oxidase activity was lost from the mouse liv ... | 1979 | 114491 |
| conformational changes of yeast trnaphe and e. coli trna2glu as indicated by different nuclease digestion patterns. | the susceptibility of yeast trnaphe and escherichia coli trna2glu to digestion by nucleases tl and sl are examined in a variety of environments, and the results are interpreted in view of the available three-dimensional structural information. significant differences are found in the digestion pattern of the two trnas using the guanosine-specific tl nuclease. in particular, differences are seen due to varying the type of salts in the environment. however, the sl nuclease results on the two trnas ... | 1979 | 114514 |
| [effect of combination of hetacillin, cephapirin and amikacin (author's transl)]. | with the investigations of the effect of combination of hetacillin, cephapirin and amikacin, following experimental results were obtained; 1. effect of combination of amikacin, cephapirin and hetacillin was differed depending upon the genus and species of bacteria. however, in general, in the cases showing effective results there was a relation in the concentration of these antibiotics. with strains of escherichia coli amikacin and cephapirin was cooperative, but not with other combinations and ... | 1979 | 114683 |
| studies on egg disinfection. | various concentrations of alkyldimethylbenzyl ammonium chloride (qac), na2co3, and ethylenediaminetetracetic acid (edta) were tested for antimicrobial activity singly and in combination against escherichia coli, arizona hinshawii, and pseudomonas aeruginosa. bactericidal activity of the reagents were evaluated in embryonating eggs, trypticase soy broth, and a medium containing lecithin. toxicity of the chemicals was assayed in embryonating eggs. an appraisal was made of an egg-washing solution c ... | 1979 | 114994 |
| [effectiveness of environmental disinfection with formalin aerosol]. | the effectiveness was tested of preventive disinfection of pig-fattening houses with formalin, applied as aerosol with the swingfog sn 100 apparatus. the effectiveness of this treatment was evaluated according to the proportion of smears containing lactoso-positive micro-organisms, expressed as a percentage of the total number of smears, and according to the reduction of contamination with e. coli and st. aureus on wooden or aluminium carriers. the effectiveness was found to be good and the trea ... | 1979 | 115138 |
| nitroblue tetrazolium (nbt) reduction by bacteria. some properties of the reaction and its possible use. | all the s. albus, e. coli and p. aeruginosa strains examined reduced nitroblue tetrazolium (nbt) to dark blue formazan. the amount of formazan produced was proportional to the number of bacteria. under the same growth conditions, an equal number of bacteria of various strains produced different amounts of formazan. however, there were statistically verified differences in the nbt-reduction between the three species examined. the nbt-reduction took place in all phases of growth but was most inten ... | 1979 | 115226 |
| bacterial plasmids: autonomous replication and vehicles for gene cloning. | the use of recombinant dna techniques in the analysis of the structure and replication of bacterial plasmids has provided much information on the properties of these genetic elements and has led to the construction of plasmid elements that are potentially very useful as gene cloning vehicles in escherichia coli and other gram-negative bacteria. the genetic and molecular properties of plasmids mini-f, cole1, and rk2 are described with particular emphasis on the origin and direction of replication ... | 1979 | 115636 |
| effects of apramycin, a novel aminoglycoside antibiotic on bacterial protein synthesis. | 1. the novel aminoglycoside antibiotic apramycin is shown to be a potent inhibitor of protein synthesis in bacteria both in vivo and in vitro. 2. in cell-free systems from escherichia coli programmed with poly(u), apramycin induces translation errors, as assayed by incorporation of leucine, isoleucine and serine, although this effect occurs only to a limited extent. 3. apramycin inhibits the translocation step of protein synthesis both in vivo, in protoplasts of bacillus megaterium, and in vitro ... | 1979 | 115690 |
| enzyme-linked immunosorbent assay for bovine immunoglobulin subclass-specific response to brucella abortus lipopolysaccharides. | an enzyme-linked immunosorbent assay was developed to follow the bovine response, by immunoglobulin class and subclass, to defined smooth and rough lipopolysaccharides (lps) of brucella abortus. binding to smooth lps of immunoglobulin g1 (igg1) and igg2 in sera from brucella-infected animals was significantly greater than binding in sera from normal uninfected animals. competition or steric blocking among igm, igg1, and igg2 for binding sites on smooth lps was shown to occur. binding of igm to b ... | 1979 | 115797 |
| the nature of the damage to escherichia coli dna induced by gamma-irradiation. | quantitative studies of the number of gamma-induced single-strand breaks (ssbs) and enzyme-labile sites (elss) were performed using the model of col e1 plasmids, which undergo transition from the covalently closed form (ccf) into the open circular form (ocf) during gamma-irradiation of the plasmid-bearing strain e. coli jc 411. by adding 0.5 medta the repair endonucleases of the cell, which effect the transition of elss into ssbs during and after gamma-irradiation, were totally inhibited. it was ... | 1979 | 115807 |
| effects of low ampicillin concentrations on penicillin sensitive and beta-lactamase producing strains of neisseria gonorrhoeae. | the effects of therapeutic concentrations of ampicillin on non-beta-lactamase and beta-lactamase producing strains of neisseria gonorrhoeae were studied. a small but significant fraction of bacteria in a gonococcal population was found to respond in a bacteriostatic rather than a bactericidal way upon ampicillin treatment. in agreement with this was the finding of morphologically unaltered cells in the scanning electron microscope after ampicillin exposure. ampicillin treatment of beta-lactamase ... | 1979 | 115831 |
| restriction enzyme analysis of bacillus subtilis ribosomal ribonucleic acid genes. | the organization of the ribosomal ribonucleic acid (rrna) genes (rdna) of bacillus subtilis was examined by cleaving the genome with several restriction endonucleases. the rdna sequences were assayed by hybridization with purified radioactive rrna's. our interpretation of the resulting electrophoretic patterns is strengthened by an analysis of a fragment of b. subtilis rdna cloned in escherichia coli. the results indicated that there are eight rrna operons in b. subtilis. each operon contains on ... | 1979 | 115842 |
| transcriptional regulation of escherichia coli k-12 major outer membrane protein 1b. | eleven independent insertion mutations were isolated that prevented expression of major outer membrane protein 1b. seven of the mutations were mucts insertions located at ombp. these ompb::mucts strains fell into two phenotypic classes with regard to expression of proteins 1a and 1b. the remaining four mutants were comprised of one tn5 and three mucts insertions mapping at par. the mucts insertions at par were used to construct fusions of the lac operon to the par promoter. expression of beta-ga ... | 1979 | 115844 |
| function of modified nucleosides 7-methylguanosine, ribothymidine, and 2-thiomethyl-n6-(isopentenyl)adenosine in procaryotic transfer ribonucleic acid. | to elucidate subtle functions of transfer ribonucleic acid (trna) modifications in protein synthesis, pairs of trna's that differ in modifications at specific positions were prepared from bacillus subtilis. the trna's differ in modifications in the anticodon loop, the extra arm, and the tuc loop. the functional properties of these species were compared in aminoacylation, as well as in initiation and peptide bond formation, at programmed ribosomes. these experiments demonstrated the following. (i ... | 1979 | 115845 |
| organization of transfer and ribosomal ribonucleic acid genes in bacillus subtilis. | the structural relationship between the transfer ribonucleic acid (trna) and the ribosomal rna (rrna) genes of bacillus subtilis has been studied by restriction endonuclease analysis of total chromosomal deoxyribonucleic acid (dna) and characterization of dna fragments cloned in escherichia coli. the dna sequences encoding rrna and trna were assayed by hybridization to radioactive rna. the results support the conclusion that the trna genes are interspersed between and closely linked to the rrna ... | 1979 | 115849 |
| nucleotide sequence of formylmethionine trna from an extreme thermophile, thermus thermophilus hb8. | the nucleotide sequence of formylmethionine trna from an extreme thermophile, thermus thermophilus hb8, was determined by a combination of classical methods using unlabeled samples to determine the sequences of the oligonucleotides of rnase t1 and rnase a digests and a rapid sequencing gel technique using 5'-32p labeled samples to determine overlapping sequences. formylmethionine trna from t. thermophilus is composed of two species, trnaf1met and trnaf2met. their nucleotide sequences are almost ... | 1979 | 115855 |
| enzyme immunoassay of pancreatic glucagon at the picogram level using beta-d-galactosidase as a label. | an enzyme immunoassay of pancreatic glucagon was established by using e. coli beta-d-galactosidease [ec 3.2.1.23] as a marker. in order to increase the sensitivity of the immunoassay, different peptides obtained from glucagon fragments were used to produce the enzyme conjugate and the immunogen. antiserum n6e raised against c-terminal fragment peptide (15-29) could be diluted to more than 1 : 100,000 in the assay and was highly specific for pancreatic glucagon. the antiserum reacted well with th ... | 1979 | 115858 |
| a novel peptidoglycan-associated lipoprotein found in the cell envelope of pseudomonas aeruginosa and escherichia coli. | protein h, one of the major outer membrane proteins pseudomonas aeruginosa, shows an interesting interaction with the peptidoglycan layer of the cell. it is retained by peptidoglycan after extraction of the cell envelope with sds solution at 35 degrees c. a protein of the same molecular weight (21,000) as protein h was found in the peptidoglycan-associated fraction of escherichia coli k-12 prepared under the same conditions. this protein, designated here as protein 21k, was purified from the cel ... | 1979 | 115860 |
| site of action of rnase i on the 50 s ribosome of escherichia coli and the association of the enzyme with the partially degraded subunit. | the 50 s ribosome of escherichia coli is partially degraded by rnase i in presence of a high concentration of mg2+ (10 to 20 mm); the partially degraded subunit becomes resistant to the further action of rnase i. the latter remains latent in association with the subparticle as in case of 30 s ribosome (neu, h.c., and heppel, l.a. (1954) proc. natl. acad. sci. u.s.a. 51, 1267-1274). as a result of nucleolytic action, 23 s rna is degraded to a smaller size and four proteins (l4, l10, l7/l12) are r ... | 1979 | 115862 |
| purification and characterization of hydroperoxidase ii of escherichia coli b. | the second heme-containing hydroperoxidase isozyme (hp-ii) has been isolated from aerobic cultures of escherichia coli b. the protein exists as a stable tetramer of subunits of equal size, with a combined molecular weight of 312,000. the heme spectrum of hp-ii is unusual, in that it exhibits two absorbance maxima at 407 and 591 nm; the alkaline pyridine hemochromogen spectrum shows maxima at 425, 559, and 609 nm. hp-ii differs in several respects from the hp-i isozyme previously reported (claibo ... | 1979 | 115870 |
| transcription termination in the escherichia coli ribosomal rna operon rrnc. | the distal portion of the escherichia coli ribosomal rna operon rrnc has been analyzed to determine (a) the transcription boundaries of an operon containing rrna genes distal to rdna, and (b) the sequence elements which dictate efficient termination of rna synthesis. i have sequenced a 505-base pair dna restriction fragment containing the genes which specify 5 s rrna, trna1asp, and trnatrp, in that order. segments of a spacer dna separate these genes: the 5 s-trna1asp and trna1asp-trnatrp spacer ... | 1979 | 115888 |
| analysis of rna secondary structure by photochemical reversal of psoralen crosslinks. | aminomethyltrioxsalen (amt), a psoralen, is known to cause interstrand crosslinks in double stranded nucleic acids. we have demonstrated the photochemical reversal of this reaction, and have used this result to develop a method for identification of specific sequences which are adjacent because of rna secondary structure formation. e. coli 5s rrna is used as a model system. we isolated and characterized a product that is derived from the stem region of 5s rna. | 1979 | 116192 |
| control features within the rpljl-rpobc transcription unit of escherichia coli. | gene fusions constructed in vitro have been used to examine transcription regulatory signals from the operon which encodes ribosomal proteins l10 and l7/12 and the rna polymerase beta and beta' subunits (the rpljl-rpobc operon). portions of this operon, which were obtained by in vitro deletions, have been placed between the ara promoter and the lacz gene in the gene-fusion plasmid pmc81 developed by m. casadaban and s. cohen. the effect of the inserted dna segment on the expression of the lacz g ... | 1979 | 116224 |
| promoter mutations in the transfer rna gene tyrt of escherichia coli. | the dna sequences for nine independent promoter mutants of a tyrosine trna gene, tyrt of escherichia coli, are reported. the nine mutations involve six transitions, two transversions, and one deletion. they are located at four different sites in the first 30 base pairs preceding the start point of transcription. the changes found are: a t.a to a.t transversion at position -8 (two mutants); a t.a to c.g transition at position -8 (three mutants); a t.a to c.g transition at position -13 (two mutant ... | 1979 | 116232 |
| [examinations on the behaviour of grampositive and gramnegative bacteria on aluminium foil (author's transl)]. | the survival times of staph. aureus, strept. faecalis, e. coli, klebs. pneumoniae, ser. marcescens and p. aeruginosa were found out on aluminium foil. it was examined whether there exists a dependence on the initial colony count and in mixed cultures a mutual influence of the several species of bacteria. the longest survival times were found among the grampositive cocci. ser. marcescens and klebs. pneumoniae could no more be indicated after 19 days in case of initial colony count of log 7.0/cm2; ... | 1979 | 116442 |
| prolactin and colibacillus-induced fluid transport in ligated intestinal segments. | two experiments, using the ligated intestinal segment technique, were conducted to determine whether the pituitary hormone prolactin (prl) could reduce escherichia coli-induced fluid loss into the small intestine of 2- to 3-week-old pigs. inoculation of 10(6) to 10(8) enteropathogenic e coli organisms into ligated jejunal segments caused a significant accumulation of luminal fluid within 12 hours. in the first experiment, intraluminal inoculation with 0.5 mg of ovine prl along with the bacteria ... | 1979 | 116577 |
| competition of beta-lactam antibiotics for the penicillin-binding proteins of pseudomonas aeruginosa, enterobacter cloacae, klebsiella aerogenes, proteus rettgeri, and escherichia coli: comparison with antibacterial activity and effects upon bacterial morphology. | the competition of a number of beta-lactam morphogenic probes for the penicillin-binding proteins (pbps) of pseudomonas aeruginosa, enterobacter cloacae, klebsiella aerogenes, proteus rettgeri, and escherichia coli has been studied. the results indicate that the various gram-negative bacteria have similar, but not identical, pbp patterns and that the individual proteins probably perform similar morphogenic functions as in e. coli k-12. comparison of the 50% binding concentrations of the compound ... | 1979 | 116592 |
| [mutations in the purine nucleoside phosphorylase (pup) gene of escherichia coli k-12 characterized by leaky damage to enzymatic activity and a pleiotropic effect]. | the phenotype of earlier obtained mutants air38 and air6 is caused by leaky mutations of the structural gene for purine nucleoside phosphorylase (pup). these mutants are unable to grow on the medium with inosine as the only carbon source in the presence of thymidine. in contrast to ordinary leaky mutations, air38 and air6 are dominant in heterozygotes. when the strain f' with mutations air38 or air6 on episomes was used for conjugational matings with f- reca (pup+), recombinants with unexpected ... | 1979 | 116903 |
| [riboflavin auxotrophs of escherichia coli]. | escherichia coli riboflavin auxotrophs having a different level of riboflavin requirement were isolated. this auxotrophic mutations are located near cysb93 and trpa62 markers. the complementary effect of bacillus subtilis riboflavin operon linked with ppr1 hybrid plasmid with rib8-1 and rib1-1 mutations was obtained. | 1979 | 116904 |
| synthesis of proteins coded by plasmid vectors of pcv series (apr, tcr) and their recombinant derivatives (pdm) in e. coli minicells. | polypeptide synthesis directed by vector plasmids of pcv series conferring ampicillin and tetracycline resistance (apr, tcr) and by recombinant plasmids (pdm) have been analyzed using the minicell system. it has been found that a polypeptide of 34 000 daltons is responsible for the tcr phenotype and regulated from the promoter near the hindiii site. cloning of dna fragments into hindiii site allowed to conclude that dna from drosophila melanogaster contains nucleotide sequences which may act as ... | 1979 | 116907 |
| [epidemiology of hospital-acquired urinary tract infections (author's transl)]. | among hospital-acquired infections, urinary tract infections are the widest spread. the most common pathogenic agents are e. coli, enterococci and proteus mirabilis. indwelling bladder catheters account for about 70% of infections. suprapubic urine drainage can reduce the incidence of urinary tract infections by approximatively 50%. | 1979 | 117318 |
| [epidemiology of hospital-acquired infections in surgery (author's transl)]. | the incidence of hospital-acquired infections in surgery fluctuates between 1.5 and 29%, the average infection is 5%. the most frequent hospital infections are: urinary tract infections, wound infections, respiratory tract infections, sepsis and infections of the skin. escherichia coli and staphylococcus aureus are most commonly isolated from the nosocomial infections. hospital infections in surgery are of endogenous origin, i.e. from the organisms of the body itself (e. g. wound infections afte ... | 1979 | 117331 |
| [synthesis of l-aspartic acid by escherichia coli and pseudomonas fluorescens as related to the cultivation conditions]. | the capacity of the cultures escherichia coli str. 85, 113, bc, c and k-12 and pseudomonas fluorescens str. 1 to synthesize l-aspartic acid from fumarate and ammonium ions was studied. e. coli str. 85 was shown to synthesize the largest amounts of aspartic acid. the cultivation conditions which helped to increase the activity several times were selected. the product of fumarate amination by ammonium ions was identified and found to be l-isomer of aspartic acid with an angle of rotation of [alpha ... | 1979 | 117446 |
| [aanalysis of resistance and its transferability in microflora from primary meat production]. | resistance spectra and their transferability were studied in g- strains isolated from the primary production of meat by the method after bohus (1974). out of the set of strains, 93% were resistant, and out of these, 65% were multiresistant. thirty-five per cent of the resistant strains were r plasmid donors. of the two recipients, the laboratory strain of e. coli ihe nx was significantly better. among the 21 determined resistance spectra, the following were the most frequent: tc, smtcsu, and tca ... | 1979 | 117592 |
| [survival of bacteria on zinc-coated and aluminum materials]. | the survival of st. aureus. e. coli, and of salmonellae was tested without any protein error and with it on new galvanized and with disinfection disturbed plate and compared with the survival of these microorganisms on new aluminous plate which was considered to be inert. the investigation by means of the impression method lasted for seven days. the growth of the microorganisms was evaluated according to the index of their survival. a distinct oligodynamic effect of the galvanized plate was prov ... | 1979 | 117598 |
| adhesion and locomotion of human leukocytes in vitro; importance of protein coating; effect of lidocain, ethanol and endotoxin. | the adhesion of leukocytes to glass beads in protein-free media was quantitatively high and not dependent on divalent cations. addition of plasma, albumin or gelatin in increasing concentrations gradually reduced leukocyte adhesion, which then became increasingly dependent on divalent cations. heat inactivation of plasma did not affect leukocyte adhesion. leukocyte migration in glass capillary tubes, which was dependent on a heat labile plasma factor, was promoted by each of the proteins listed ... | 1979 | 117672 |
| effect of divalent cation concentrations on the antibiotic susceptibilities of nonfermenters other than pseudomonas aeruginosa. | the effects of supplementing mueller-hinton broth with calcium and magnesium on the minimal inhibitory concentrations (mics) of eight aminoglycosides, colistin, tetracycline, and carbenicillin for 11 nonfermenters other than pseudomonas aeruginosa were studied and compared with the effects for escherichia coli and p. aeruginosa. mics were simultaneously performed in unsupplemented mueller-hinton broth and mueller-hinton broth supplemented to contain 5 mg of calcium and 2.5 mg of magnesium per dl ... | 1979 | 117746 |
| inhibitory effects of carcinogenic mycotoxins on deoxyribonucleic acid-dependent ribonucleic acid polymerase and ribonuclease h. | fourteen mycotoxins were tested for inhibitory effects on ribonucleic acid polymerase of rat liver and escherichia coli and nuclear ribonuclease h of rat liver and tetrahymena pyriformis. these enzymes were strongly inhibited by (-)-luteoskyrin, (+)-rugulosin, patulin, and pr toxin. | 1979 | 117749 |
| affinity labeling of a reactive sulfhydryl residue at the peptidyl transferase p site in drosophila ribosomes. | an affinity label has been prepared that is specific for the p site of a eucaryotic peptidyl transferase, that of drosophila melanogaster. it has the sequence c-a-c-c-a-(ac[3h]leu) with a mercury atom added at the c-5 position of all three cytosine residues (referred to as the mercurated fragment). this label is an analogue of the 3' terminus of n-acetylleucyl-trna. the mercurated fragment binds specifically to the p site of peptidyl transferase. it participates fully in peptide bond formation a ... | 1979 | 117829 |
| studies on the primary structure of escherichia coli 23s rrna. iii. nucleotide sequence of the t1 rnase digestion products containing one or no uridine residue. | | 1979 | 117844 |
| [protein kinase activity in escherichia coli]. | when growing e. coli in a minimal medium, at least four proteins from the soluble fraction and one ribosome-associated protein are found phosphorylated at the level of their threonine and serine residues. | 1979 | 117936 |
| requirement for ribosomal protein bm-l11 in stringent control of rna synthesis in bacillus megaterium. | a spontaneously occurring thiostrepton-resistant mutant of bacillus megaterium has been shown to yield ribosomes lacking protein bm-l11, a protein immunologically related to escherichia coli ribosomal protein l11. here we have demonstrated that the mutant strain has acquired the relaxed phenotype and is unable to synthesise guanosine tetraphosphate and pentaphosphate in vivo. ribosomes from the mutant strain are unable to support the synthesis of these two compounds in vitro, but this deficiency ... | 1979 | 118006 |
| modification of the guanine in the invariant sequence 5' ccg44aac3' of the escherichia coli 5 s rna in solution by kethoxal. | | 1979 | 118053 |
| mutagenic and dna-damaging effects of n-alkyl-n-(alpha-acetoxyalkyl)nitrosamines, models for metabolically activated n,n-dialkylnitrosamines. | mutagenic and dna-damaging effects of a series of n,n-dialkylnitrosamines monosubstituted at the alpha-carbon with an acetoxyl group were tested in salmonella typhimurium, escherichia coli, and bacilus subtilis in the absence of metabolic activation system. the compounds comprised 8 n-alkyl-n-(acetoxymethyl)nitrosamines (alkyl=methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, or tert-butyl) and n-butyl-n-(1-acetoxybutyl)nitrosamine. all the compounds, except one with a tert-butyl gro ... | 1979 | 118074 |
| construction and characterization of e. coli promoter-probe plasmid vectors. i. cloning of promoter-containing dna fragments. | derivatives of the escherichia coli drug-resistance plasmid pbr316 have been constructed which act as molecular probes for promoter-containing dna restriction fragments from various prokaryotic genomes. the plasmids, designated pbrh1 and pbrh3b, contain a unique ecori restriction site located within the promoter for the tetracycline resistance (tcr) gene. this site was created by the insertion of a chemically synthesized octanucleotide, containing the ecori cleavage sequence, into the hindiii si ... | 1979 | 118083 |
| outer membrane of pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins. | a number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of pseudomonas aeruginosa. it was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was determined by the solubilization temperature. four of these heat-modifiable proteins had characteristics similar to protein ii of t ... | 1979 | 118160 |
| in vivo methylation of elongation factor tu of escherichia coli. | a protein existing mainly in the supernatant fraction of escherichia coli was found to be methylated by accepting the methyl moiety originating from methionine. the protein was identified as peptide synthesis elongation factor tu (ef-tu) by the following criteria. 1) the methylatable protein separated at the same position as purified ef-tu on two-dimensional gel electrophoresis. 2) the methylatable protein interacted with antiserum specific for ef-tu. amino acid analysis of the methyl-labeled pr ... | 1979 | 118166 |
| the automicrobic system for urines. | an evaluation of the automicrobic system (ams) for urines (vitek systems, inc.) was carried out under the auspices of the product evaluation committee of the college of american pathologists from the period june 1977 through october 1978. data generated during this evaluation indicated that, when comparing the ams methodology to our clinical microbiology laboratory methodology, a 37% time saving could be realized by utilizing the ams. quantitation with the ams showed a 99% correlation with the c ... | 1979 | 118176 |
| virulence factors in bacillus thuringiensis: purification and properties of a protein inhibitor of immunity in insects. | we have previously shown that bacillus thuringiensis subsp. alesti, serotype 3, produces two extracellular inhibitors of the immune system of saturniid pupae (designated inhibitors a and b; edlund et al., 1976). starting from the culture supernatant of a new mutant of b. thuringiensis with a decreased extracellular proteolytic activity, we have now purified immune inhibitor a(ina). the procedure described consists of three steps: ultrafiltration, precipitation with ammonium sulphate and chromato ... | 1979 | 118233 |
| influence of subcellular fractions of mammalian testes on the mutagenic activity of nitrofurans toward escherichia coli; presence of a co-mutagen-like factor. | the activation of nitrofurans to mutagenic intermediates by testicular tissue was investigated. af-2 and nitrofurazone were tested in a microsomal suspension assay with strain e. coli k-12 343/113 as indicator and subcellular fractions from rabbit testes. different mutation patterns were observed in the presence or absence of testicular homogenate, indicating the presence of different mutagenic intermediates. the frequency of arg+ reversion increased proportionally to the homogenate concentratio ... | 1979 | 118378 |
| attenuation in the escherichia coli tryptophan operon: role of rna secondary structure involving the tryptophan codon region. | the secondary structure of the terminated trp leader transcript from escherichia coli was analyzed by rnase t1 partial digestion. base-paired regions were recovered by nondenaturing gel electrophoresis and identified by denaturing gel electrophoresis and fingerprinting. the tandem tryptophan codons in the leader peptide coding region were found to be base paired with a more distal region of the transcript. this and other secondary structures that the trp leader rna can form help explain the phys ... | 1979 | 118451 |
| sodium acetate as a preservative in protein hydrolysate solutions. | the inhibitory effect of sodium acetate on microorganism growth in protein hydrolysate solutions was studied. solutions of 5% protein hydrolysate and 5% dextrose in water (seven parts) and 50% dextose in water (three parts) containing 0, 30, 50 and 90 meq/liter of sodium acetate were inoculated with staphylococcus aureus, escherichia coli, candida albicans and pseudomonas aeruginosa. the number of colony-forming units in the solutions after inoculation was compared with that after incubation for ... | 1979 | 118672 |
| minimal inhibitory concentrations of 34 antimicrobial agents for control strains escherichia coli atcc 25922 and pseudomonas aeruginosa atcc 27853. | the use of control strains of bacteria is important to monitor the accuracy and precision of antimicrobial susceptibility testing. knowledge of the minimal inhibitory concentrations of commonly used organisms would be useful to achieve a degree of inter- as well as intra-laboratory reproducibility. the minimal inhibitory concentrations of 34 antimicrobial agents for control strains escherichia coli atcc 25922 and pseudomonas aeruginosa atcc 27853, as determined by a microdilution method in catio ... | 1979 | 118705 |
| sublethal stress in escherichia coli: a function of salinity. | sublethal stress in escherichia coli was detected in various test media after exposure (in vitro) to seawater of various salinites. stress was measured with an electrochemical detection technique and a beta-galactosidase assay. test media included ec medium, medium a-1, and tryptic soy broth modified to contain lactose for beta-galactosidase assay experiments. stress was defined as the difference between a predicted electrochemical response time calculated for unstarved cells from a standard cur ... | 1979 | 118708 |
| use of lac operon fusions to isolate escherichia coli mutants with altered expression of the fumarate reductase system in response to substrate and respiratory controls. | | 1979 | 118757 |
| [expression of a bacterial gene, cloned in the yeast, saccharomyces cerevisiae]. | vectors allowing cloning of foreign d.n.a. in the yeast saccharomyces cerevisiae have been recently described. we have introduced in this yeast the lac z gene of the bacteria escherichia coli. an active beta-galactosidase, which is absent in the recipient strain, has been detected in transformed yeast. we thus conclude that the bacterial lac z gene is expressed in yeast. we further showed that the enzyme found in the transformed yeast is identical to the bacterial enzyme with respect to size and ... | 1979 | 118813 |
| cyanine dye as monitor of membrane potentials in escherichia coli cells and membrane vesicles. | the fluorescence response of a positively charged cyanine dye: 3,3'-dimethylindodicarbocyanine iodide can be specifically related to the generation in escherichia coli cells and e. coli membrane vesicles of an electrical membrane potential induced either by substrate oxidation or by an artificially imposed potassium diffusion gradient. the energy-dependent quenching of the dye fluorescence correlates well with the known effect on delta phi of: oxidation of various energy sources, external ph and ... | 1979 | 118877 |
| in vitro and in vivo laboratory studies on three hydroxyiminophenylacetyl cephalosporins with particular reference to sk&f 80303, an unusually long-acting cephalosporin. | three cephalosporins with 7-(2-hydroxyiminophenylacetamido) side chains (sk&f 79433, 80000 and 80303), differing in their 3-substituents, exhibited similar broad-spectrum antibacterial activity in vitro against strains of staphylococcus aureus, streptococcus faecalis and various gram-negative bacilli. all three were active in vivo (s.c., mouse) against s. aureus, escherichia coli or klebsiella pneumoniae, but they differed significantly in serum pharmacokinetic profiles. sk&f 80303 produced high ... | 1979 | 118958 |
| comparative bactericidal activities of beta-lactam antibiotics determined in agar and broth media. | comparative bactericidal activities were determined utilizing a relatively large number of test strains, in both agar and broth media, with special reference to the time of exposure of the bacteria to certain beta-lactam antibiotics. it was apparent that the activities increase with time. the concentrations producing a 99.9% kill with cephalothin for escherichia coli, klebsiella sp., and carbenicillin for pseudomonas aeruginosa were higher in broth than in agar. in contrast, those of benzylpenic ... | 1979 | 118959 |
| construction of a colony bank of e. coli containing hybrid plasmids representative of the bacillus subtilis 168 genome. expression of functions harbored by the recombinant plasmids in b. subtilis. | a collection of about 2500 clones containing hybrid plasmids representative of nearly the entire genome of b. subtilis 168 was established in e. coli sk1592 by using the poly(da).poly(dt) joining method with randomly sheared dna fragments and plasmid phv33, a bifunctional vector which can replicate in both e. coli and b. subtilis. detection of cloned recombinant dna molecules was based on the insertional inactivation of the tc gene occurring at the unique bamhi cleavage site present in the vecto ... | 1979 | 119126 |
| comparison of three procedures for isolating dna from bacteria. | three methods employing chloroform-isoamyl alcohol (ci), phenol, or enzymes, were evaluated for isolating dna from escherichia coli, bacillus subtilis, and arthrobacter globiformis. for the amounts of reagents employed at optimum conditions in the ci and phenol procedures, 0.4-0.9 mg of dna/g wet weight of cells was isolated. using the enzymatic procedure, approximately twice as much dna was isolated. dna isolated by the ci procedure contained 0.03-0.09% protein and 0.08-0.12% rna. dna isolated ... | 1979 | 119130 |
| [effect of aerobic and anaerobic germs on the healing of decubitus ulcers]. | bacteriological examinations of decubitus ulcers were performed in 34 geriatric patients. a total of 179 wound swabs were analyzed for aerobic and anaerobic bacteria. the decubitus ulcers were divided into three groups according to wound healing: group a with progressive worsening, group b, stationary, and group c with healing within 10 weeks. the aerobic bacteria isolated from the three groups were significantly different (p less than 0.0001). in group a pseudomonas aeruginosa was isolated in 8 ... | 1979 | 119316 |
| [development of enterococci and coli bacteria in bulgarian yogurt]. | the effect of lactic acid fermentation in bulgarian yoghourt was studied by the development and decline of hygiene level indicating microorganisms--enterococci and coli-bacteriae. it was established that for the first 6 h after production of bulgarian yoghourt, the quantity of enterococci (str. faecalis, resp. str. faecium) increases 5--6 times on the average, after the 12th hour it begins slowly to decrease and the number is reduced two times during storage for 7 days at 7--10 degrees c tempera ... | 1979 | 119346 |
| prolonged shock in the monkey following live e coli organism infusion. | responses of the rhesus monkey to the administration of live escherichia coli organisms during an observation of 0--27 hours were studied. nine monkeys were infused for 30 minutes with live e coli organisms, the dose ranging between 7.6 x 10(9) and 3.0 x 10(11) organisms/kg. three of nine animals survived for 24 hours or longer. nonsurvivors demonstrated significant hypotension, hypoglycemia, and hypoinsulinemia, while survivors showed lesser degrees of physiologic derangement. findings were hep ... | 1979 | 119585 |
| on the biological role of ribosomal protein bm-l11 of bacillus megaterium, homologous with escherichia coli ribosomal protein l11. | | 1979 | 119869 |
| probable mechanism of enzyme evolution: how did ebg of e. coli originate? | a mechanism is proposed for the formation of ebg-evolved beta galactosidase-of e. coli based on the following assumptions: 1. in the presence of lactose, certain proteins being translated bind to their m-rna-ribosome complexes; 2. this binding interferes with the release of m-rna from the bacterial chromosome, marking the gene; 3. thereupon a cytosine specific methylase and methyl cytosine deaminase pair, modify - mutate - the marked gene; 4. the result, after five or so mutations, is a new gene ... | 1979 | 119900 |
| failure of hyperalimentation to enhance survival in malnourished rats with e. coli-hemoglobin adjuvant peritonitis. | | 1979 | 120032 |
| experimental syphilis in the rabbit: passive transfer of immunity with immunoglobulin g from immune serum. | a preparation of immunoglobulin g isolated from a pool of immune sera derived from rabbits with long-term syphilis was shown to possess a high degree of purity as judged by immunodiffusion and protein electrophoresis. the antitreponemal power of the preparation of immunoglobulin g and that of the immune serum pool from which it was derived were found to be equivalent in both the skin protection and the systemic protection test. the observation that neither normal serum nor a pool of serum derive ... | 1979 | 120386 |
| the growth and form of bacterial colonies. | a simple method is described for measuring the profile of bacterial colonies. profiles were determined for colonies of bacillus cereus, escherichia coli and staphylococcus albus of different ages. in spite of differences in cell morphology, the colony profiles had a common basic structure consisting of steeply rising leading edge connected by a ridge to an interior region where height also rose, though less steeply, to a flat or domed centre. the colony mass increased exponentially through part ... | 1979 | 120410 |
| e. coli ribosomal proteins are cross reactive with antibody prepared against chlamydomonas reinhardi chloroplast ribosomal subunit. | antisera prepared against purified chlamydomonas reinhardi small chloroplast ribosomal subunit, judged homogenous by sucrose gradient velocity sedimentation and rna gel electrophoresis was immunologically cross reactive with e. coli ribosomal proteins. the results of three different experimental approaches, namely ouchterlony double diffusion, sucrose gradient velocity sedimentation and two dimensional crossed immunoelectrophoresis indicate that both e. coli ribosomal subunits and the chloroplas ... | 1979 | 120481 |