| [helicobacter pylori and nonsteroidal antirheumatics]. | the frequency of occurrence of helicobacter pylori in the antral mucosa was investigated prospectively in a group of 66 patients (17 men, 49 women, mean age 58 +/- 8.4 years) who had been treated with nonsteroidal anti-rheumatic drugs and 33 controls (14 men, 19 women, mean age 60.7 +/- 6.6 years) who had not received these drugs. in the first group the indication for gastroscopy was ingestion of nonsteroidal antirheumatic drugs for at least 8 weeks, irrespective of dyspeptic symptoms (present i ... | 1990 | 2379465 |
| urea protects helicobacter (campylobacter) pylori from the bactericidal effect of acid. | colonization of the stomach with helicobacter (campylobacter) pylori is common in patients with duodenal ulcer disease, which is known for its high acid secretion. although the bacterium is usually isolated by culture of a gastric biopsy specimen, viable organisms may sometimes be found in the acidic gastric juice. it was postulated that urease, by generating ammonia, protected h. pylori from acid. to test this hypothesis, the ph susceptibility of h. pylori, proteus mirabilis, and the urease-neg ... | 1990 | 2379775 |
| antibody to cytotoxin in infection by helicobacter pylori. | gastrointestinal disease and colonization by helicobacter pylori were determined in 36 asymptomatic volunteers and 30 symptomatic individuals undergoing endoscopy and biopsy. serum antibody immunoglobulin g (igg) and iga to h. pylori were measured by enzyme-linked immunosorbent assay. serum antibody to a cytotoxin produced by h. pylori was detected with a neutralization assay. serum igg was 95% predictive of infection by h. pylori, and serum iga was 88% predictive. antibody to the cytotoxin was ... | 1990 | 2380350 |
| use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli. | a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ... | 1990 | 2380381 |
| comparison of elisa antigen preparations alone or in combination for serodiagnosing helicobacter pylori infections. | the immunoglobulin g antibody response to helicobacter pylori was assessed in 78 patients with non-ulcer dyspepsia using five different antigen preparations. all patients were endoscoped and biopsied. the h pylori state was determined histologically on at least two endoscopic biopsy specimens using a modified giemsa stain. the ultracentrifuged cell sonicate, acid glycine extract, and 120 kilodalton protein antigens were specific in diagnosing infection (95-98%), but had only moderate sensitivity ... | 1990 | 2380396 |
| reproducible test for detecting helicobacter pylori in frozen samples. | | 1990 | 2380399 |
| helicobacter pylori-related gastroduodenal disease in children. diagnostic utility of enzyme-linked immunosorbent assay. | to evaluate the accuracy of igg and iga serological tests in establishing a diagnosis of helicobacter (campylobacter) pylori gastric infection, 60 children presenting with chronic abdominal pain were prospectively studied. endoscopic antral biopsies were obtained and analyzed for the presence of h. pylori using three standard methods: culture and identification of bacterial isolates, microscopic examination for morphologically characteristic bacteria, and urease production by the biopsy specimen ... | 1990 | 2384045 |
| animal model of helicobacter pylori infection. | | 1990 | 2386377 |
| helicobacter pylori and gastritis in patients with peptic ulcer and non-ulcer dyspepsia: ethnic differences in singapore. | peptic ulcer occurs with different frequencies in the three main racial groups in singapore. this study aimed firstly to determine the prevalence of helicobacter pylori in peptic ulcer and non-ulcer dyspepsia patients of the different races and secondly, to assess the relation between h pylori, histological gastritis, patient diagnosis, and race. gastric antral biopsy specimens from 1502 patients undergoing gastroduodenoscopy were studied and 892 (59%) were positive for h pylori. h pylori was st ... | 1990 | 2387503 |
| quantitative histological study of mucosal inflammatory cell densities in endoscopic duodenal biopsy specimens from dyspeptic patients using computer linked image analysis. | inflammatory cell counting in endoscopic biopsy sections was carried out on duodenal mucosal samples from defined sites in patients with duodenal ulcer, duodenitis but no ulcer, non-ulcer dyspepsia, and asymptomatic controls using computer linked image analysis. the variables measured included polymorphonuclear and mononuclear cells per mm of superficial epithelium and per mm2 lamina propria. duodenal ulcer crater margin and mucosal biopsy specimens from endoscopically inflamed mucosa in the gro ... | 1990 | 2387505 |
| in vitro surface properties of the newly recognized gastric pathogen helicobacter pylori. | there appears to be a particular association between helicobacter pylori and the gastric antrum, but the mechanisms by which the organism adheres to and colonizes the gastric mucosa are unclear. surface hydrophobicity and surface charge mediate the adherence of other bacterial pathogens to mucosal epithelial cell surfaces. therefore, in this study we characterized both the surface hydrophobicity and the surface charge of 10 h. pylori strains grown in broth culture. four complementary methods wer ... | 1990 | 2387633 |
| nodular gastritis and helicobacter pylori. | numerous reports have established the association of helicobacter pylori and peptic ulcer disease in adults. recently, this association has also been demonstrated in children. we investigated 14 children and adolescents with recurrent abdominal pain. in six patients, endoscopy revealed gastritis and helicobacter pylori was identified. giemsa stain was more sensitive than culture or urease testing in identifying the bacteria. in four of the six, a nodular appearance of the antral mucosa was obser ... | 1990 | 2388131 |
| [state of local and total humoral immunity in duodenal ulcer with campylobacter pylori infection]. | a total of 83 patients with duodenal ulcer and a varying degree of gastric mucosa contamination with c. pylori were examined. secretory iga was less frequently detectable in the gastric juice of patients with higher level of gastric mucosa contamination with c. pylori and in lower concentrations than in the patients with a lesser c. pylori contamination. healing of duodenal ulcer defects was associated with a decrease of gastric mucosa contamination and elevation of secretory iga content in the ... | 1990 | 2389200 |
| helicobacter pylori and bismuth subnitrate. | | 1990 | 2389738 |
| [does campylobacter helicobacter pylori infection have a clinical relevance? methodologic, epidemiologic and clinical studies]. | the authors searched for campylobacter pylori (cp) in gastric biopsies from 180 patients by means of microbiological culture. warthin-starry staining and urease activity determination. 50 patients with cp-positive antral gastritis were treated with bismuthsubsalicylate 2.4 g per day for 3 weeks, followed by a therapy-free interval of 7-10 days and then a control biopsy was performed. combined results of bacterial culture and histology proved to be mostly reliable. the prevalence of cp in hungary ... | 1990 | 2390166 |
| campylobacter pylori infection in uremic dialyzed patients. eradication of the infection by colloidal bismuth subcitrate. | | 1990 | 2392203 |
| [helicobacter pylori and gastroduodenal pathology in the republic of panama]. | 129 patients, panamanians and north americans, with chronic dyspepsia, were prospectively studied regarding infection by h. pylori and the type of gastroduodenal pathology diagnosed by endoscopy and histology. the rapid urea split test was compared with the culture. the percentage of patients infected by h. pylori increased with age. patients under 30, 37%; 31 to 40, 61%; 41 to 76, 75%. h. pylori was found in 70% of the panamanians and 54% of the americans. h. pylori infected 81%, 75%, 54% and 2 ... | 1990 | 2392579 |
| eradication of helicobacter mustelae from the ferret stomach: an animal model of helicobacter (campylobacter) pylori chemotherapy. | colonization of the ferret stomach by helicobacter mustelae has been suggested as a possible animal model for helicobacter pylori-associated gastroduodenal disease of humans. our study was designed to determine whether antimicrobial chemotherapy could eradicate h. mustelae from ferrets. triple antimicrobial therapy combining amoxicillin, metronidazole, and bismuth subsalicylate was successful in eradicating the organism from 5 of 7 (71%) adult ferrets. despite apparent in vitro susceptibility, n ... | 1990 | 2393285 |
| another important effect of helicobacter pylori infection. | | 1990 | 2394340 |
| [the frequency of detecting campylobacter pylori in biopsies of the gastric mucosa]. | campylobacter pylori were detected in 70% of patients with diseases of the digestive tract. it was found endoscopically that presence of campylobacter pylori is always accompanied by changes of gastric mucosa, mainly in the antral region. there were no specific symptoms characteristic of campylobacter pylori gastritis. there was a close correlation between the presence of campylobacter pylori and activity of the inflammatory process (active gastritis in 73.6% of patients). | 1990 | 2396383 |
| [effect of alcohol and cirrhosis on the presence of helicobacter pylori in the gastric mucosa]. | the aim of this study was to determine whether there was any relationship between alcohol consumption, cirrhosis and helicobacter pylori associated antral gastritis. one hundred and forty-four patients undergoing upper gastrointestinal endoscopy were prospectively included and classified in four groups. the first group of 23 patients had cirrhosis and an alcohol consumption below 80 g per day. the second group of 31 patients had cirrhosis and an alcohol consumption over 80 g per day. the third g ... | 1990 | 2397864 |
| [epidemiology of helicobacter pylori infection]. | the endemic behaviour of helicobacter pylori (h.p., formerly known as campylobacter pylori) among the population of the town (57,000 inhabitants) and rural district (116,000 inhabitants) of landshut was investigated with consideration being given to nationality, socio-economic status, housing and the local supply of drinking water. the study involved 894 patients, whose consecutive gastric biopsies were sent to the pathological institute of the municipal hospital in landshut. the overall h.p. in ... | 1990 | 2398773 |
| ciprofloxacin in the treatment of helicobacter pylori in patients with gastritis and peptic ulcer. | | 1990 | 2401802 |
| helicobacter pylori, peptic ulcer, and cimetidine. | | 1990 | 2402163 |
| effect of abo blood group and secretor status on the frequency of helicobacter pylori antibodies. | duodenal ulcer is associated with such genetic characteristics as blood group o and secretor status. since helicobacter pylori has been proved to be an important factor in the pathogenesis of duodenal ulcer, we wanted to study whether the frequency of h. pylori antibodies would vary in individuals with different blood group antigens. antibodies against h. pylori were determined in 271 blood donors. acid glycine extract from an h. pylori strain was used as antigen in enzyme immunoassay. our resul ... | 1990 | 2402586 |
| differences in helicobacter pylori associated gastritis in the antrum and body of the stomach. | in 1,265 patients with helicobacter pylori (hp)-associated gastritis, the degree of hp colonisation correlated in a highly significant manner with the degree and the activity of gastritis in the antrum and body. the degree of hp colonisation is identical in both body and antrum in 49.3%, the antrum being more markedly colonised in 38.7% and the body in 12.0%. in comparison, the degree and activity of gastritis is more pronounced in the antrum in 71.3% and 72.5%, respectively, the corresponding f ... | 1990 | 2402931 |
| campylobacter pylori and gastroduodenal disease. | campylobacter pylori is a newly described, spiral-shaped, gram-negative bacillus that is oxidase positive, catalase positive, and urease positive and grows slowly in culture. although observed in human tissue at the beginning of the century, it was not cultured until 1982. because there are significant morphological and genetic differences between this organism and other species of campylobacter, it will probably be reclassified in a new genus. current information indicates that the organism pri ... | 1990 | 2404565 |
| [campylobacter pylori, gastritis and ulcer. where are we going now?]. | studies in human volunteers as well as animals clearly indicate that campylobacter pylori is a key component in the development of type b-gastritis as well as in stomach- and duodenal ulcer disease. it seams likely that a better knowledge in how this microbe colonize the stomach mucosa will soon give us new and better possibilities to prevent and treat these diseases and their relapses. | 1990 | 2405351 |
| microbiological aspects of helicobacter pylori (campylobacter pylori). | the human gastric pathogen campylobacter pylori has recently been reclassified as helicobacter pylori, and a related spiral bacterium found in the stomach of ferrets has been designated helicobacter mustelae. the general microbiological features of helicobacter pylori are delineated here, with details of phenotypic differences between helicobacter pylori and helicobacter mustelae; comparisons are made with wolinella succinogenes and campylobacter jejuni. the helicobacter organisms possess an ext ... | 1990 | 2406141 |
| diagnosis of campylobacter pylori infections: the "gold standard" and the alternatives. | both direct and indirect techniques are available for the detection of gastric infections due to campylobacter pylori. these techniques vary in their degree of efficacy, invasiveness, and convenience. they fall into five major categories, based on either the particular characteristic of the c. pylori organism or the manifestation of the c. pylori infection that they detect. the five categories include histologic examination of culture biopsy specimens, serologic examination, rapid urease tests, ... | 1990 | 2406850 |
| effect of oral bismuth subsalicylate on campylobacter pylori and on healing and relapse rate of peptic ulcer. | a controlled, open clinical trial was performed to compare the effect of oral bismuth subsalicylate (600 mg three times daily) with that of cimetidine (800 mg/d) on campylobacter pylori, ulcer healing, and rate of ulcer relapse in 49 patients with peptic ulcer. at the first endoscopic examination (to confirm the diagnosis) and at the second (after 4 weeks of treatment), biopsy specimens were taken from the gastric antrum; the presence of c. pylori in culture and the rate of ulcer healing were de ... | 1990 | 2406852 |
| campylobacter pylori: its link to gastritis and peptic ulcer disease. | bismuth salts have been used to treat acid peptic disease for 150 years. recently, the discovery of campylobacter pylori and the observation that this bacterium is inhibited by bismuth salts have regenerated interest in the antibacterial properties of bismuth. bismuth and heavy metals inhibit growth of all campylobacter species and many enteric anaerobes. bismuth alone cures c. pylori infection in only 30% of patients, but in combination with other broad-spectrum antibiotics, it can achieve a cu ... | 1990 | 2406862 |
| bismuth subsalicylate in the treatment of gastritis due to campylobacter pylori. | fifty patients completed an investigator-blind trial comparing bismuth subsalicylate, erythromycin ethylsuccinate, and placebo matched to the bismuth salt in the treatment of gastritis associated with campylobacter pylori. c. pylori was cleared from 14 (77.8%) of 18 patients given locally active bismuth, from one (6.7%) of 15 patients given erythromycin, and from none of 17 patients given placebo. gastritis resolved in 13 (81%) of 16 patients treated with bismuth but in only three of 13 receivin ... | 1990 | 2406863 |
| epidemiology and pathophysiology of campylobacter pylori infections. | since the first isolation of campylobacter pylori in australia in 1982, this bacterium has been isolated from persons in all parts of the world. although initially recognized in patients with gastrointestinal symptoms, c. pylori can also be isolated from apparently asymptomatic persons. c. pylori infection is infrequent in young children in developed countries; during adulthood c. pylori infection becomes progressively more frequent, a phenomenon that parallels the age distribution of type b gas ... | 1990 | 2406864 |
| acridine-orange to identify campylobacter pyloridis in formalin fixed, paraffin-embedded gastric biopsies. | | 1986 | 2417073 |
| detection of campylobacter pyloridis in gastric mucosa by phase contrast microscopy. | | 1986 | 2419365 |
| simplified techniques for identifying campylobacter pyloridis. | | 1986 | 2432094 |
| isolation and characterization of campylobacter pyloridis from gastric biopsies. | gastric biopsy specimens were examined microbiologically and histologically for the presence of campylobacter pyloridis. of 51 randomly selected patients, 22 (43%) were found to harbor c. pyloridis in the gastric mucosa. the histologic demonstration of spiral organisms observed by staining with hematoxylin and eosin correlated well with microbiologic isolation of the organisms. there was a strong association (95.5%) between c. pyloridis in the gastric mucosa and histologically defined gastritis. ... | 1987 | 2432774 |
| campylobacter pyloridis gastritis i: detection of urease as a marker of bacterial colonization and gastritis. | a method to detect the presence of campylobacter pyloridis in dyspeptic patients is described. the test procedure involves placing a gastric pinch biopsy into a small amount of a solution containing urea and a ph indicator in the well of a microtiter tray. the method depends on the ability of c. pyloridis to hydrolyse urea and release an alkaline product (ammonia). the "microtiter biopsy urease test" is 100% specific for c. pyloridis and has a 91% sensitivity after 18 h reaction time. seventy-fi ... | 1987 | 2436470 |
| campylobacter pyloridis gastritis ii: distribution of bacteria and associated inflammation in the gastroduodenal environment. | the distribution of campylobacter pyloridis in the stomach and duodenal bulb of two series of patients (n = 53) with dyspeptic symptoms was studied. in all patients where a comprehensive search of the stomach was conducted, c. pyloridis and active chronic gastritis were associated. that is, the presence of this organism anywhere in the stomach may be taken as a marker of active chronic gastritis within that stomach, although not necessarily at the site of bacterial detection. two main patterns o ... | 1987 | 2436471 |
| simple half-gram stain for showing presence of campylobacter pyloridis in sections. | | 1987 | 2440912 |
| rapid staining of campylobacter pyloridis. | | 1987 | 2447004 |
| campylobacter pylori in swedish patients referred for gastroscopy. | campylobacter pylori was isolated more often from patient with peptic ulcers and in an age-related manner in a material of 395 consecutive patients referred for gastroscopy. direct microscopy was done with gram and acridine orange stain and found too insensitive for practical use. all patients were investigated serologically with an enzyme immunoassay which showed excellent correlation with positive cultures for c. pylori. the findings were discussed, and the enzyme immunoassay, with a negative ... | 1988 | 2449900 |
| improved visualisation of mucus penetration by campylobacter pylori using a brown-hopps stain. | | 1988 | 2450896 |
| campylobacter pylori--acridine orange stain and ultraviolet fluorescence. | | 1988 | 2453440 |
| campylobacter pylori detected by indirect immunohistochemical technique. | an immunohistochemical assay for staining c. pylori is described. the method is compared with cultivation of c. pylori and observation of campylobacter-like organisms (clos) in hematoxyline-eosine (he) stained sections. eighteen biopsies from which c. pylori was cultivated but not seen in he stained sections and three culture negative biopsies with clos seen in he stained sections were selected from 331 biopsies including 113 culture positive biopsies. there were agreements between cultivation o ... | 1988 | 2456087 |
| culture of the organisms and histochemical identification. | the detection of campylobacter pylori in the upper gastrointestinal tract has important implications for the pathogenesis and treatment of peptic disease. c. pylori is best isolated from biopsies using blood agar or chocolate blood agar incorporating a selective supplement. plates should be incubated under microaerophilic conditions for five days. a gram's stain of a biopsy smear provides a more rapid result but the organism may be patchy in its distribution. of the histochemical methods used, t ... | 1988 | 2458624 |
| n-acetylneuraminyllactose-binding fibrillar hemagglutinin of campylobacter pylori: a putative colonization factor antigen. | campylobacter pylori is the causative agent of gastritis and possibly of peptic and duodenal ulcers in adults. histological observations show c. pylori attached to gastric epithelium as well as in the mucus layer of the stomach. we found that clinical isolates of c. pylori possess a cell-bound hemagglutinin detectable with human erythrocytes (all phenotypes tested) and those of a variety of animal species. the c. pylori hemagglutinin is antigenic, heat sensitive, and destroyed by pronase and pap ... | 1988 | 2459065 |
| [comparison of methods for the identification of campylobacter pylori in gastric biopsies of patients with dyspepsia]. | campylobacter pylori has been associated with gastro-duodenal inflammatory disease. ninety-five adults with dyspepsia were examined for the presence of c. pylori in the gastric antrum and near gastric or duodenal ulcers (when present) by means of culture, gram and acridine orange stains, and urease activity of biopsies. c. pylori was identified from 51 out of 67 patients with chronic gastritis, from 9 out of 9 patients with duodenal ulcer, and from 8 out of 10 patients with gastric ulcer. acridi ... | 1987 | 2459724 |
| evaluation of staining methods for identifying campylobacter pylori. | campylobacter pylori has been implicated in the pathogenesis of peptide ulcer disease. the rapid identification of this organism may depend upon histologic diagnosis, because culture methods are complex and require a minimum of seven days in order to identify a negative specimen. the purpose of this study was to determine which stain used to identify this organism was the most cost-effective and easiest to perform and interpret on a routine basis. sixty-one consecutive gastric antral biopsies we ... | 1988 | 2459956 |
| rapid diagnosis of campylobacter pylori by gram's stain. | campylobacter pylori (cp) is implicated as a probable pathogen in gastritis and peptic ulcer disease. a blinded prospective study of 112 subjects evaluated how gram's-stained touch preparations of mucosal biopsies compared with culture, routinely processed hematoxylin and eosin (h and e) and warthin-starry (ws) staining in confirming the presence of cp. at endoscopic examination, two mucosal biopsies were taken from the gastric antrum and two from the fundus of each subject. one biopsy from each ... | 1988 | 2459961 |
| [histologic detection of campylobacter pylori]. | | 1989 | 2466286 |
| [assessment of methods of rapid diagnosis of campylobacter pylori in duodenal ulcers]. | a study of diagnostic value of the campylobacter pylori test as well as microbiological, bacteriological and biochemical findings in 134 patients with gastroduodenal ulcer and 20 healthy persons indicates that association of the campylobacter pylori and biochemical tests are reliable and may be recommended for wide use in practical public health. | 1989 | 2469254 |
| the association of campylobacter pylori with mucosal pathological changes in a population at risk for gastric cancer. | in the study of a group of 178 unselected patients (105 men, 73 women, mean age 44.5 years), from a population at high risk for gastric carcinoma, who presented with chronic dyspepsia, a minimum of 8 gastric and oesophageal biopsy specimens were taken during upper gastro-intestinal endoscopy, and examined histologically and histochemically for the presence of campylobacter pylori and other pathological lesions. gastric colonisation by c. pylori was found in 75% of men and 68.4% of women. in 90% ... | 1989 | 2470157 |
| colloidal bismuth subcitrate (de-nol) inhibits degradation of gastric mucus by campylobacter pylori protease. | there is increased awareness that infection with campylobacter pylori could be a major factor in the pathogenesis of gastric disease. here, we present evidence that the extracellular protease elaborated by this bacteria, which causes degradation of gastric mucus, is inhibited by an antiulcer agent, colloidal bismuth subcitrate (cbs; de-nol). the study was conducted with c. pylori cultured from antral mucosal biopsy specimens of patients undergoing gastroscopy. the grown colonies of bacteria were ... | 1989 | 2470250 |
| receptor-mediated adherence of campylobacter pylori to mouse y-1 adrenal cell monolayers. | an in vitro adherence assay was developed to study the adherence of campylobacter pylori to mammalian cells. strains of c. pylori were isolated from individuals with gastritis. these strains possessed the fibrillar n-acetylneuraminyllactose(neuraminlactose)-binding hemagglutinin (nlbh) and were found to adhere to monolayers of mouse y-1 adrenal cells. adherence was rapid, prevented by pretreatment of the y-1 cells with clostridium perfringens neuraminidase, and blocked by the neuraminlactose-con ... | 1989 | 2473033 |
| campylobacter pylori infection in biopsy specimens of gastric antrum: laboratory diagnosis and estimation of sampling error. | campylobacter pylori infection was sought in 382 consecutive patients referred for upper gastrointestinal endoscopy. five antral biopsy specimens were taken from each patient: one was inserted into a clo-test to detect the urease activity of c pylori, two were sent for histological analysis where multiple sections were stained by the warthin-starry silver method, and two were sent for microbiological evaluation by gram stain and culture. a patient was deemed to be infected when c pylori was cult ... | 1989 | 2474579 |
| [a method of determining campylobacter pylori in the gastric mucosa in peptic ulcer and chronic gastritis]. | an original method is described for the detection of campylobacter pylori in gastric mucosa biopsy specimens in chronic gastritis and gastroduodenal ulcers. the method is based on the detection (by a special test) of extremely high urease activity of c. pylori. the method has been employed in examinations of 211 patients. the results and prospects for the use of this technique for the detection of c. pylori in patients with gastroduodenal diseases are discussed. | 1989 | 2474707 |
| [detection of campylobacter pylori in children with chronic gastritis]. | examinations of the gastric mucosa biopsy specimens from 38 children suffering from chronic gastritis have revealed campylobacter bacteria in 21 (55.3%). studies of the cultural, morphologic, and biochemical characteristics of the isolated strains have identified them as c. pylori. | 1989 | 2474708 |
| comparison of rapid urease tests, staining techniques, and growth on different solid media for detection of campylobacter pylori. | thirty-nine single antral biopsies (phase 1) and 99 sets of six antral biopsies (phase 2) were collected from 132 patients, and 87 (63%) yielded positive cultures for campylobacter pylori. of several primary media tested in phase 1, tryptic soy agar and skirrow agar, each supplemented with 10% whole sheep blood, supported relatively good growth of c. pylori. in phase 2, four of the six biopsies in each set were tested with different urease systems. selective urea agar for rapid identification wa ... | 1989 | 2475524 |
| [diagnostic value of various methods of detection of campylobacter pylori in patients with gastroduodenal pathology]. | | 1989 | 2478756 |
| [a rapid method of diagnosis of gastric and duodenal campylobacter pylori infections]. | | 1989 | 2478757 |
| the prevalence of campylobacter pylori in gastric biopsies from cancer patients. | the prevalence of campylobacter pylori (cp) in gastric biopsies from cancer patients has not been previously studied. we reviewed 112 gastric biopsies from 78 adult patients at a cancer hospital. the patients had a previous history or current diagnosis of gastric or esophageal carcinoma (26 patients), gastric lymphoma (15 patients), gastric stromal tumor (three patients), carcinoid tumor (two patients), nongastroesophageal malignancy (29 patients), or atrophic gastritis with nonhealing ulcer (th ... | 1989 | 2479007 |
| the reliability of urease tests, histology and culture in the diagnosis of campylobacter pylori infection. | the accuracy of various staining techniques for immersion microscopy, of five media for culture and five urease test modalities for the detection of campylobacter pylori infection is reported. it was found that 2% urea unbuffered gel preparation is the most accurate urease test (sensitivity: 89%, specificity: 98%) but a minimal amount of 10,000 cfu/ml is necessary to observe positivity and the sensitivity of urease tests drops to 52% in patients under antimicrobial treatment. for histological di ... | 1989 | 2479086 |
| an endoscopic study of campylobacter pylori in an israeli population. | | 1989 | 2480339 |
| oligonucleotide probe for detection and identification of campylobacter pylori. | we have developed a novel and practical dna-rna hybridization assay for the detection and identification of campylobacter pylori in the gastric mucosa. this technique utilizes a [32p]ddatp-labeled synthetic oligonucleotide probe complementary to a nucleotide sequence present in c. pylori 16s rrna. this probe is very sensitive and reacted with all 23 strains of c. pylori tested. it is also highly specific, since there was no cross-reactivity with the heterologous organisms campylobacter coli, c. ... | 1989 | 2480360 |
| [comparative study of the frequency of campylobacter pylori in duodenal and stomach ulcers and chronic gastritis using the urease test and histologic staining]. | | 1989 | 2480691 |
| [frequency of campylobacter pylori in antral biopsies of patients with indications for upper digestive endoscopy]. | | 1988 | 2484118 |
| [the urease test for the diagnosis of campylobacter pylori infection]. | to evaluate the usefulness of urea media routinely applied in the diagnosis of campylobacter pylori infection, 50 patients with gastroduodenal pathology were studied. two biopsies of antral mucosa were taken; one of them was placed on urea agar base (difco) prepared without agar, and the other in saline for gram staining and culture in selective and enriched media. in 39 patients campylobacter pylori was found by gram staining and/or culture. 38 of them were urease test positive, 24 (61.5%) duri ... | 1989 | 2485236 |
| isolation of campylobacter pyloridis from nigerian patients with gastroduodenal pathology. | recent reports have indicated that campylobacter pyloridis may be a causative factor in upper digestive pathology. gastric and duodenal biopies were taken from 144 patients undergoing upper digestive endoscopy as part of investigation for dyspeptic symptoms at the endoscopy unit of lagos university teaching hospital between october, 1985 and november, 1986 employing acmi gastroduodenoscope model tx-7. the first 50 specimens were placed in 3 ml of brain heart infusion and taken to the microbiolog ... | 1989 | 2486780 |
| campylobacter pylori in upper digestive tract diseases in children. | results of studies on the frequency of the occurrence of the bacteria, campylobacter pylori, in the mucous membrane of the gastric antrum are presented in this paper. the study was carried out on 61 children treated for chronic abdominal pain. the diagnosis was established on the basis of flexible endoscopy and histology of antral biopsies. the presence of campylobacter pylori was determined using the clo-test. the positive clo-test was obtained in 87.5% of children with gastritis, in 75% of chi ... | 1989 | 2489238 |
| [in vitro activity of 16 antimicrobial agents against helicobacter (campylobacter) pylori]. | campylobacter pylori has been associated with the etiology of gastritis and duodenal ulcer. it has been shown that several drugs, among them a variety of antimicrobials, eliminate c. pylori from gastric mucosa at least for a time, resulting in an improvement of the patients' symptoms. the activity of 16 antimicrobials (ampicillin, cefazolin, cefuroxime, cefotaxime, imipenem, aztreonam, tigemonam, erythromycin, vancomycin, nalidixic acid, colistin , norfloxacin, ciprofloxacin, difloxacin, ofloxac ... | 1989 | 2490433 |
| [pathogenicity of campylobacter pylori in the digestive tract]. | | 1989 | 2490635 |
| animal models for campylobacter pylori infection. | | 1989 | 2490934 |
| campylobacter pylori absence in extragastric human intestinal sites and animal stomachs. | | 1989 | 2491306 |
| peptic ulcer: from epidemiology to cause. | any postulate that attempts to explain the aetiology of peptic ulcer must take into consideration a number of established facts. (i) at the beginning of the 20th century there was a steep rise in the incidence of peptic ulcers. over the past 2 decades, trends have shown a significant decline in some western countries, such as the united kingdom, and a significant rise in certain asian countries, such as hong kong and singapore. (ii) there are marked geographical variations in incidence (for exam ... | 1989 | 2491356 |
| [campylobacter pylori and the gastric mucosa. apropos of the detection of campylobacter pylori in 12 patients with gastric mucosa lesions]. | the authors report the results of systematic research on campylobacter pylori during high endoscopy. of the 60 patients examined, 12 were found to have the germ in their antral, gastric mucosa. all these patients had a history of chronic gastritis. they recall the role of this germ in creating chronic gastritis, and evoke the hypothesis that the mucosa becomes fragile facilitating the appearance of an ulcer. | 1989 | 2491383 |
| campylobacter pylori gastritis: long term results of treatment with amoxycillin. | to evaluate the efficacy of amoxycillin in eradicating campylobacter pylori, endoscopic biopsy specimens were taken from the antral mucosa of 40 children with gastritis before, immediately after, and (in 30 patients) three months after treatment. immediately after treatment 34 patients (85%) no longer had the organism in the mucosa, and the gastritis had healed in 23 (58%). three months later the infection had recurred in 22 of 30 patients (73%), and the gastritis had relapsed in all of them. si ... | 1989 | 2495776 |
| biotypes of campylobacter pylori isolated in gastroduodenal biopsies. | | 1989 | 2498104 |
| use of the mouse for the isolation and investigation of stomach-associated, spiral-helical shaped bacteria from man and other animals. | spiral-helical shaped bacteria other than campylobacter pylori have been shown to infect the human stomach. the characteristic helical morphology of these bacteria appears to be similar to that of bacteria found in the stomachs of many other animal species. early reports on gastric bacteria suggested that rodents may be useful for investigation and isolation of stomach-associated bacteria. therefore, anaesthetised mice were given, through a stomach tube, a heavy suspension of a spiral-helical ba ... | 1989 | 2498521 |
| dna-dna hybridization incompatibility of campylobacter pylori with other campylobacter and wolinella species. | dna-dna hybridization in solution was used to characterize 23 human isolates of campylobacter pylori. the 23 isolates showed dna affinity with the type strain (nctc 11637). the relative binding ratios varied between 0.83 and 1. type strains of c. coli (nctc 11366), c. jejuni (nctc 11351), c. laridis (nctc 11352), c. sputorum subsp. sputorum (atcc 35980), wolinella recta (nctc 11489) and w. succinogenes (atcc 29543) showed relative binding ratios less than 0.01 compared to the c. pylori type stra ... | 1989 | 2499346 |
| seroepidemiological study of the immune response to campylobacter pylori in potential risk groups. | to gain more understanding of the epidemiology of campylobacter pylori infection, the immune response to the organism was studied in the following selected potential risk groups: endoscopy staff (n = 45), dental staff (n = 58), orphanage children (n = 24), psychiatric patients (n = 58), and family contacts of campylobacter pylori-infected patients (n = 55). the frequency of an igg and iga antibody response in the different groups was determined by the immunoblot method and compared with that in ... | 1989 | 2506018 |
| influence of media supplements on growth and survival of campylobacter pylori. | experiments were designed to determine the role of heme and the importance of other factors in the growth of campylobacter pylori. campylobacter pylori strains were tested for their ability to synthesize porphyrin, for their ability to grow and be maintained on basal medium and basal medium supplemented with blood or blood products, and for the influence of bovine serum albumin and catalase on viability. results indicated that campylobacter pylori does not require heme as a source of porphyrin. ... | 1989 | 2506019 |
| haemolytic activity of campylobacter pylori. | the haemolytic activity of several clinical and reference strains of campylobacter pylori was determined using cell-free preparations of broth-grown organisms and human, horse, guinea pig, rabbit and sheep erythrocytes. significant levels of haemolysis were produced only when the cell-free preparations were concentrated tenfold. however, three of 14 strains still gave haemolysis values of less than 50% when tested with guinea pig erythrocytes. significant haemolytic activity could not be demonst ... | 1989 | 2506037 |
| lactobacillus acidophilus inhibits growth of campylobacter pylori in vitro. | campylobacter pylori has been implicated as a causative factor in acid-peptic disease. lactobacillus acidophilus is known to inhibit the growth of pathogens in the human gastrointestinal tract. we recovered c. pylori from gastric antral biopsies of seven patients with acid-peptic disease; the isolates were then cultured in brucella broth. the effect of l. acidophilus (cultured in deman-rogosa-sharpe broth) on the growth of c. pylori was tested by a mixed culture technique. l. acidophilus inhibit ... | 1989 | 2511224 |
| campylobacter pylori, duodenal ulcer disease, and gastrin. | | 1989 | 2511976 |
| effect of varying ph on the susceptibility of campylobacter pylori to antimicrobial agents. | the susceptibility of 22 clinical isolates of campylobacter pylori to eight antimicrobial agents was studied under varying ph conditions. macrolides (erythromycin, dirythromycin), clindamycin and to a lesser extent quinolones lost efficacy at lowered ph. the activity of ampicillin and metronidazole remained relatively stable throughout the ph range tested. the effect of ph an antimicrobial efficacy may warrant consideration when selecting an antibiotic to clear campylobacter pylori in vivo, sinc ... | 1989 | 2512133 |
| antral helicobacter pylori, hypergastrinaemia, and duodenal ulcers: effect of eradicating the organism. | | 1989 | 2514864 |
| review article: antibiotics and the gut. | antibiotics have an important place in the management of gastrointestinal disease. recent studies have demonstrated efficacy in acute bacterial gastroenteritis caused by salmonellae and campylobacteriaceae, shigellae and enterotoxigenic strains of e coli (etec). tetracycline remains effective in cholera. antibiotic resistance is widespread amongst the enteric pathogens and can quickly spread during epidemics of infective diarrhoeas. it is important that antibiotics are reserved for the treatment ... | 1989 | 2518846 |
| campylobacter pylori infection and gastric mucosal lesions in patients with chronic liver diseases. | | 1989 | 2519092 |
| [isolation of campylobacter pylori from gastric biopsies in people from the south of chile]. | | 1989 | 2519366 |
| nsaids: new approaches to limiting gastropathy. | an extensive literature search on non-steroidal anti-inflammatory drug (nsaid)-induced gastropathy in rheumatic conditions has been carried out. a reduced incidence of gastropathy has been observed among newly developed nsaids such as etodolac and the non-acidic nabumetone. an alternative prophylactic therapy to avoid nsaid-induced gastroduodenal mucosal damage which has been successfully tested in several trials is co-medication with the prostaglandin analogue misoprostol. the cytoprotective ag ... | 1989 | 2525273 |
| [frequency of campylobacter pylori in apparently healthy and asymptomatic venezuelans]. | we evaluated 21 healthy asymptomatic venezuelan volunteers free of risk factors for gastritis with upper gastrointestinal endoscopy, and four antral biopsies: a)2 for histological examinations, b) urease test, c) culture and gram coloration. 13 out of 21 subjects were positive for campylobacter pylori (61.30%) all of them had chronic gastritis and in the negative cases 7 were considered histologically normal. urease test was positive in 12/13 with a sensitivity of 92% and 100% specificity. frequ ... | 1989 | 2535448 |
| [comparison of urease test (clotest) and histology in the diagnosis of campylobacter pylori]. | campylobacter pylori has been implicated in the pathogenesis of peptic ulcer, non-ulcer dyspepsia and chronic gastritis. c. pylori produces large amounts of urease which has been used as a biochemical support to identify the microorganism. in this study, we described the use of a rapid urease test (clotest) to detect c. pylori. in 46 consecutive patients, biopsy of fundus and antrum were obtained for histology and clotest. all specimens showed chronic gastritis. in 22 patients (48%) c. pylori wa ... | 1989 | 2535449 |
| seroepidemiology of campylobacter pylori infection in various populations. | campylobacter pylori infection has been recognized as being strongly associated with chronic gastritis and duodenal ulceration, but the prevalence of c. pylori infection in a normal population is not known. a serological survey was conducted in four countries with different geographical and socioeconomic status, in a randomly chosen population as representative as possible, by using an enzyme-linked immunosorbent assay (elisa) with a sonic extract of two strains as the antigen. the test had a sp ... | 1989 | 2549098 |
| in-vitro susceptibility of campylobacter pylori to quinolones. | | 1989 | 2551872 |
| duodenal ulcer and campylobacter pylori. | | 1989 | 2563492 |
| duodenal ulcer relapse after eradication of campylobacter pylori. | | 1989 | 2563535 |
| routine cleaning and the elimination of campylobacter pylori from endoscopic biopsy forceps. | the effect of routine cleaning in removing campylobacter pylori from the biopsy forceps of endoscopes has been examined in a series of 50 patients. campylobacter pylori was isolated from the biopsies of 15 of the patients, while one of the 50 biopsy forceps washings yielded the organism after routine cleaning. this study suggests that there is a small chance of transmitting c. pylori by endoscopic equipment if cleaning is the only method of decontamination adopted. | 1989 | 2564022 |
| amoxycillin plus tinidazole for campylobacter pylori gastritis in children: assessment by serum igg antibody, pepsinogen i, and gastrin levels. | 32 children (mean age 12 years, range 6-18) with non-specific abdominal pain and campylobacter pylori positive gastritis received a six week course of daily oral amoxycillin (50 mg/kg) and tinidazole (20 mg/kg). before treatment and one month after stopping treatment, endoscopic biopsy samples were taken from the antral mucosa and serum c pylori igg antibody, pepsinogen i, and gastrin levels were measured in fasting blood samples. one month after treatment 30 children (94%) were cleared of c pyl ... | 1989 | 2564507 |