| inhibition of host- and bacteria-derived proteinases by natural anthocyanins. | santos j, la vd, bergeron c, grenier d. inhibition of host- and bacteria-derived proteinases by natural anthocyanins. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01372.x. © 2011 john wiley & sons a/s background and objectives: host- and bacteria-derived proteinases are considered to play critical roles in periodontitis progression. this study investigated the ability of a blackcurrant extract and its major anthocyanins (cyanidin-3-o-glucoside, cyanidin-3-o-rutinoside and delphinidin-3-o ... | 2011 | 21517858 |
| bacterial diversity in persistent periapical lesions on root-filled teeth. | the purpose of this study was to analyze the bacterial diversity in persistent apical lesions on root-filled teeth by using culture-independent molecular methods. | 2009 | 21523213 |
| porphyromonas gingivalis-dendritic cell interactions: consequences for coronary artery disease. | an estimated 80 million us adults have one or more types of cardiovascular diseases. atherosclerosis is the single most important contributor to cardiovascular diseases; however, only 50% of atherosclerosis patients have currently identified risk factors. chronic periodontitis, a common inflammatory disease, is linked to an increased cardiovascular risk. dendritic cells (dcs) are potent antigen presenting cells that infiltrate arterial walls and may destabilize atherosclerotic plaques in cardiov ... | 2010 | 21523219 |
| preterm low birthweight and the role of oral bacteria. | preterm and low birthweight (ptlbw) continues to be a major cause of mortality and morbidity across the world. in recent years, maternal periodontal disease has been implicated as a risk factor for adverse pregnancy outcomes. there is conflicting evidence to support such an outcome as illustrated by descriptive, case control and randomised controlled trials involving pregnant women from across the world, using different measurement tools to determine the level of periodontal disease. whilst cons ... | 2010 | 21523222 |
| differential effects of periopathogens on host protease inhibitors slpi, elafin, scca1, and scca2. | secretory leukocyte peptidase inhibitors (slpi), elafin, squamous cell carcinoma antigen 1 and 2 (scca1 and scca2) are specific endogenous serine protease inhibitors expressed by epithelial cells that prevent tissue damage from excessive proteolytic enzyme activity due to inflammation. to determine the effects of various periopathogens on these protease inhibitors, we utilized human gingival epithelial cells (gecs) challenged with cell-free bacteria supernatants of various periopathogens porphyr ... | 2010 | 21523231 |
| prevalence of sos-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons. | abstract: | 2011 | 21529368 |
| chey3 of borrelia burgdorferi is the key response regulator essential for chemotaxis and forms a long-lived phosphorylated intermediate. | spirochetes have a unique cell structure: these bacteria have internal periplasmic flagella subterminally attached at each cell end. how spirochetes coordinate the rotation of the periplasmic flagella for chemotaxis is poorly understood. in other bacteria, modulating flagellar rotation is essential for chemotaxis, and phosphorylation-dephosphorylation of the response regulator chey plays a key role in regulating this rotary motion. the genome of the lyme disease spirochete borrelia burgdorferi c ... | 2011 | 21531807 |
| dendritic cells, antibodies reactive with oxldl, and inflammation. | periodontitis appears to promote chronic inflammatory diseases, including atherosclerosis, but relevant mechanisms need clarification. oral bacteria induce antibodies that bind not only bacteria, but also oxldl. porphyromonas gingivalis and aggregatibacter actinomycetemcomitans induce remarkable igg responses that are dominated by igg2, and igg2 is ifn-γ-dependent and is promoted by dendritic cells (dcs). ldl-reactive antibodies induced by p. gingivalis and a. actinomycetemcomitans include anti- ... | 2011 | 21531918 |
| the diguanylate cyclase, rrp1, regulates critical steps in the enzootic cycle of the lyme disease spirochetes. | rrp1 is the sole c-di-gmp-producing protein (diguanylate cyclase) of borrelia burgdorferi. to test the hypothesis that rrp1 regulates critical processes involved in the transmission of spirochetes between ticks and mammals, an rrp1 deletion mutant (b31-δrrp1) and a strain that constitutively produces elevated levels of rrp1 (b31-ov) were constructed. the strains were assessed for progression through the enzootic cycle using an ixodes tick/c3h-hej mouse model and tick immersion feeding methods. b ... | 2011 | 21542866 |
| effect of periodontopathogen lipopolysaccharides and proinflammatory cytokines on cd46, cd55, and cd59 gene/protein expression by oral epithelial cells. | membrane-anchored complement regulatory proteins (crps), including cd46, cd55, and cd59, protect host cells from complement attack. in the present study, we investigated whether periodontopathogen lipopolysaccharide and proinflammatory cytokines modulate crp gene/protein expression in human oral epithelial cells. the lipopolysaccharide of treponema denticola and tannerella forsythia were the most potent for increasing the gene expression of cd55 and cd59, and to a lesser extent cd46, after a 48- ... | 2011 | 21545652 |
| streptococcus mutans strains recovered from caries-active or caries-free individuals differ in sensitivity to host antimicrobial peptides. | antimicrobial peptides (amps) are among the repertoire of host innate immune defenses. in the oral cavity, several amps are present in saliva and have antimicrobial activities against oral bacteria, including streptococcus mutans, a primary etiological agent of dental caries. in this study, we hypothesized that unique s. mutans strains, as determined by dna fingerprinting from sixty 13-year-old subjects with or without experience of caries, would have different susceptibilities to α-defensins-1- ... | 2011 | 21545696 |
| treponema denticola pure is a bacterial air carboxylase. | de novo purine biosynthesis proceeds by two divergent paths. in bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (air) is converted to 4-carboxy-air (cair) by two enzymes: n(5)-carboxy-air (n(5)-cair) synthetase (purk) and n(5)-cair mutase (class i pure). in animals, the conversion of air to cair requires a single enzyme, air carboxylase (class ii pure). the cair carboxylate derives from bicarbonate or co(2), respectively. class i pure is a promising antimicrobial target. class i an ... | 2011 | 21548610 |
| identification of mono- or poly-specific monoclonal antibody to porphyromonas gingivalis heat-shock protein 60. | the aim of this study was to define the immunoreactive specificity of porphyromonas gingivalis (p. gingivalis) heat shock protein (hsp) 60 in periodontitis and atherosclerosis. | 2011 | 21556254 |
| diagnostic evaluation of a nanobody with picomolar affinity toward the protease rgpb from porphyromonas gingivalis. | porphyromonas gingivalis is one of the major periodontitis-causing pathogens. p. gingivalis secretes a group of proteases termed gingipains, and in this study we have used the rgpb gingipain as a biomarker for p. gingivalis. we constructed a naive camel nanobody library and used phage display to select one nanobody toward rgpb with picomolar affinity. the nanobody was used in an inhibition assay for detection of rgpb in buffer as well as in saliva. the nanobody was highly specific for rgpb given ... | 2011 | 21569755 |
| metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. | production and chemicals or fuels directly from co(2) is an attractive approach to solving the energy and environmental problems. 1-butanol, a chemical feedstock and potential fuel, has been produced by fermentation of carbohydrates, both in native clostridium species and various engineered hosts. to produce 1-butanol from co(2), we transferred a modified coa-dependent 1-butanol production pathway into a cyanobacterium, synechococcus elongatus pcc 7942. we demonstrated the activity of each enzym ... | 2011 | 21569861 |
| disruption of a type ii endonuclease (tde0911) enables treponema denticola atcc 35405 strain to accept an unmethylated shuttle vector. | the oral spirochete treponema denticola is associated with human periodontal disease. t. denticola atcc 35405 (td(35405)) and 33520 (td(33520)) are two routinely used laboratory strains. compared to td(33520), td(35405) is more virulent but less accessible to genetic manipulations. for instance, the shuttle vectors of td(33520) cannot be transformed into td(35405). the lack of a shuttle vector has been a barrier to study the biology and virulence of td(35405). in this report, we hypothesize that ... | 2011 | 21602384 |
| levels of serum igg specific to the bspa protein of tannerella forsythia are related to the periodontal status. | background: tannerella forsythia is a gram-negative anaerobe implicated in the development of periodontal disease (pd). bspa is a surface expressed and secreted protein, recognized as an important virulence factor of t. forsythia. this study was undertaken to determine if t. forsythia bacterial surface protein a (bspa) induces an antibody response in pd. we hypothesized that serum immunoglobulin g (igg) antibody levels against bspa will correlate with patient's disease. methods: sera were obtain ... | 2011 | 21609257 |
| levels of serum igg specific to the bspa protein of tannerella forsythia are related to the periodontal status. | background: tannerella forsythia is a gram-negative anaerobe implicated in the development of periodontal disease (pd). bspa is a surface expressed and secreted protein, recognized as an important virulence factor of t. forsythia. this study was undertaken to determine if t. forsythia bacterial surface protein a (bspa) induces an antibody response in pd. we hypothesized that serum immunoglobulin g (igg) antibody levels against bspa will correlate with patient's disease. methods: sera were obtain ... | 2011 | 21609257 |
| identification of periodontal pathogens in healthy periimplant sites. | : the aim of this study was to evaluate the presence of periodontopathogens in subgingival periimplant sites in partially edentulous patients using polymerase chain reaction procedures, with regard to areas with clinical and radiographic signs of health and areas presenting periimplant disease. | 2011 | 21613949 |
| riboswitch regulates a thiamine pyrophosphate (tpp) abc transporter of the oral spirochete treponema denticola. | thiamine pyrophosphate (tpp), a biologically active form of thiamine (vitamin b1), is an essential cofactor in all living systems. microorganisms either synthesize tpp via de novo biosynthesis pathways or uptake exogenous thiamine from the environment via specific transporters. the oral spirochete treponema denticola is an important pathogen that is associated with human periodontal diseases. it lacks a de novo tpp biosynthesis pathway and needs exogenous tpp for the growth, suggesting that it m ... | 2011 | 21622748 |
| experimental alveolitis in rats: microbiological, acute phase response and histometric characterization of delayed alveolar healing. | the pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed. | 2011 | 21625744 |
| tlr2 signaling and th2 responses drive tannerella forsythia-induced periodontal bone loss. | periodontal disease (pd) is a chronic inflammation of the tooth-supporting soft tissue and alveolar bone due to infection by a select group of gram-negative microbes, which leads to tooth loss if untreated. because mice deficient in cd4(+) cells are resistant to infection-induced alveolar bone loss, th cells have been implicated in bone-destructive processes during pd. however, the extent to which different th cell subtypes play roles in pathogenesis or host protection remains to be defined and ... | 2011 | 21632710 |
| the pathogenic persona of community-associated oral streptococci. | the mitis group streptococci (mgs) are widespread in the oral cavity and are traditionally associated with oral health. however, these organisms have many attributes that contribute to the development of pathogenic oral communities. mgs adhere rapidly to saliva-coated tooth surfaces, thereby providing an attachment substratum for more overtly pathogenic organisms such as porphyromonas gingivalis, and the two species assemble into heterotypic communities. close physical association facilitates ph ... | 2011 | 21635580 |
| crystallization of the factor h-binding protein, fhbb, from the periopathogen treponema denticola. | treponema denticola is a primary etiological agent of periodontal disease. t. denticola evades complement-mediated killing by binding to the host's factor h (fh), a negative regulator of the alternative complement pathway. the t. denticola fh-binding protein has been identified and designated as factor h-binding protein b (fhbb). crystals of recombinant fhbb were obtained by the hanging-drop vapor-diffusion method using sodium citrate and 0.2 m sodium thiocyanate. fhbb crystals diffracted to 1.8 ... | 2011 | 21636910 |
| complete genome sequence of treponema paraluiscuniculi, strain cuniculi a: the loss of infectivity to humans is associated with genome decay. | treponema paraluiscuniculi is the causative agent of rabbit venereal spirochetosis. it is not infectious to humans, although its genome structure is very closely related to other pathogenic treponema species including treponema pallidum subspecies pallidum, the etiological agent of syphilis. in this study, the genome sequence of treponema paraluiscuniculi, strain cuniculi a, was determined by a combination of several high-throughput sequencing strategies. whereas the overall size (1,133,390 bp), ... | 2011 | 21655244 |
| diversity and plasticity of the intracellular plant pathogen and insect symbiont, 'candidatus liberibacter asiaticus', revealed by hyper variable prophage genes with intragenic tandem repeats. | 'candidatus liberibacter asiaticus' (las) is a psyllid-transmitted, phloem-limited alpha proteobacterium, and the most prevalent species of liberibacter associated with a devastating worldwide citrus disease known as huanglongbing (hlb). two related and hyper variable genes (hyvi and hyvii) were identified in the prophage regions of the psy62 las genome. sequence analyses of the hyvi/hyvii genes in 35 las dna isolates collected globally revealed hyvi gene contains up to12 nearly identical tandem ... | 2011 | 21784907 |
| evaluation of local drug-delivery system containing 2% whole turmeric gel used as an adjunct to scaling and root planing in chronic periodontitis: a clinical and microbiological study. | to compare the effect of experimental local-drug delivery system containing 2% whole turmeric (gel form) as an adjunct to scaling and root planing (srp) with the effect achieved using srp alone by assessing their respective effects on plaque, gingival inflammation, bleeding on probing pocket depth, relative attachment levels and trypsin-like enzyme activity of "red complex" microorganisms, namely, bacteroides forsythus, porphvromonas gingivalis and treponema denticola. | 2011 | 21772719 |
| detection of periodontal markers in chronic periodontitis. | the aim was to compare the detection frequency of periodontopathogens by using the pado test 4.5 and checkerboard dna-dna hybridization technique in chronic periodontitis patients.thirty patients with chronic periodontitis were tested cross-sectionally with dna/rna oligogenomic probe method (iai pado test 4.5) and dna/dna whole genomic probe (checkerboard) method. samples were taken by two paper points at the deepest site in each of the four quadrants and pooled into one sample for each of the t ... | 2011 | 21769304 |
| characterization of novel ompa-like protein of leptospira interrogans that binds extracellular matrix molecules and plasminogen. | leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease of human and veterinary concern. the identification of novel proteins that mediate host-pathogen interactions is important for understanding the bacterial pathogenesis as well as to identify protective antigens that would help fight the disease. we describe in this work the cloning, expression, purification and characterization of three predicted leptospiral membrane proteins, lic10258, lic12880 (lp30) and lic12 ... | 2011 | 21755014 |
| getting the measure of syphilis: qpcr to better understand early infection. | objectives until recently, pcr had been used to detect but not quantify treponema pallidum. to understand infection kinetics of this uncultivable organism, a real-time pcr assay was developed to quantify 47 kda membrane lipoprotein gene dna (tpp47). methods assay specificity was determined against dna from humans, skin organisms and sexually transmitted pathogens. tpp47 dna (nichols strain) was used to construct a standard curve for t pallidum quantification. blood and ulcer samples were ... | 2011 | 21752804 |
| caloric restriction and chronic inflammatory diseases. | oral diseases (2011) a reduction in calorie intake [caloric restriction (cr)] appears to consistently decrease the biological rate of aging in a variety of organisms as well as protect against age-associated diseases including chronic inflammatory disorders such as cardiovascular disease and diabetes. although the mechanisms behind this observation are not fully understood, identification of the main metabolic pathways affected by cr has generated interest in finding molecular targets that could ... | 2011 | 21749581 |
| caloric restriction and chronic inflammatory diseases. | oral diseases (2011) a reduction in calorie intake [caloric restriction (cr)] appears to consistently decrease the biological rate of aging in a variety of organisms as well as protect against age-associated diseases including chronic inflammatory disorders such as cardiovascular disease and diabetes. although the mechanisms behind this observation are not fully understood, identification of the main metabolic pathways affected by cr has generated interest in finding molecular targets that could ... | 2011 | 21749581 |
| clinical and microbiological results 12 months after scaling and root planing with different irrigation solutions in patients with moderate chronic periodontitis ?a pilot randomized trial. | aim: the aim of this study was to determine in a randomized trial the impact on treatment outcome after 12 months of different subgingival irrigation solutions during scaling and root planing (srp). materials and methods: 51 adult volunteers with generalized chronic periodontitis were treated by full mouth srp using 0.9 % sodium chloride, 0.12 % chlorhexidine digluconate or 7.5 % povidone-iodine for subgingival irrigation during srp. prior to srp, after three and twelve months probing depth (pd) ... | 2011 | 21749169 |
| analysis of the peri-implant microbiota in 90 dental implants and its relationship to crevicular fluid volume. | objective: to evaluate the presence within the peri-implant sulcus of tannerela forsythia (tf), porphyromonas gingivales (pg), treponema denticola (td) and aggregatibacter actinomycetemcomitans (aa), and relate these bacteria to the peri-implant crevicular fluid volume (picfv). material and method: a prospective and cross-sectional clinical case series study was made. for the measurement of crevicular fluid, use was made of the periotron® 8000 (proflow incorporated. new york, usa), measuring the ... | 2011 | 21743411 |
| diabetes and periodontitis. | the main aim of this review is to update the reader with practical knowledge concerning the relationship between diabetes mellitus and periodontal diseases. exclusive data is available on the association between these two chronic diseases till date. articles published on this relationship often provide the knowledge of definitions of diabetes mellitus and periodontal diseases, prevalence, extent, severity of periodontal disease, complications of diabetes along with the possible underlying mechan ... | 2010 | 21731243 |
| microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment. | abstract objective: to evaluate changes in subgingival microbiota before and during the leveling and alignment orthodontic stage using the polymerase chain reaction (pcr) method. materials and methods: thirty orthodontic patients (17 females and 13 males; aged 16.7 -¦ 6.5-áy) were included in this study. subgingival microbial samples were taken from the disto-buccal gingival crevice of the left upper central incisors, the left lower central incisors, the mesio-buccal gingival crevice of the left ... | 2011 | 21827233 |
| [prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis]. | helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori. | 2011 | 21688602 |
| clinical, microbiologic, and immunologic factors of orthodontic treatment-induced gingival enlargement. | the aim of this study was to investigate the microbiologic and immunologic factors related to orthodontic treatment-induced gingival enlargement (ge). | 2011 | 21724088 |
| comparative genome analysis and genome-guided physiological analysis of roseobacter litoralis. | roseobacter litoralis och149, the type species of the genus, and roseobacter denitrificans och114 were the first described organisms of the roseobacter clade, an ecologically important group of marine bacteria. both species were isolated from seaweed and are able to perform aerobic anoxygenic photosynthesis. | 2011 | 21693016 |
| alzheimer's disease - a neurospirochetosis. analysis of the evidence following koch's and hill's criteria. | abstract: it is established that chronic spirochetal infection can cause slowly progressive dementia, brain atrophy and amyloid deposition in late neurosyphilis. recently it has been suggested that various types of spirochetes, in an analogous way to treponema pallidum, could cause dementia and may be involved in the pathogenesis of alzheimer's disease (ad). here, we review all data available in the literature on the detection of spirochetes in ad and critically analyze the association and causa ... | 2011 | 21816039 |
| synergistic virulence of porphyromonas gingivalis and treponema denticola in a murine periodontitis model. | chronic periodontitis is characterized by the destruction of the tissues supporting the teeth and has been associated with the presence of a subgingival polymicrobial biofilm containing porphyromonas gingivalis and treponema denticola. we have investigated the potential synergistic virulence of p. gingivalis and t. denticola using a murine experimental model of periodontitis. an inoculation regime of four intra-oral doses of 1 +ù 10(10) p. gingivalis cells induced significant periodontal bone lo ... | 2011 | 21729244 |
| Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy. | BACKGROUND: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two. OBJECTIVE: To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples. MATERIALS AND METHODS: Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normot ... | 2011 | 21830010 |
| Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA). | The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated. | 2011 | 21711368 |
| filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by porphyromonas gingivalis. | filifactor alocis, a gram-positive anaerobic rod, is one of the most abundant bacteria identified in the periodontal pockets of periodontitis patients. there is a gap in our understanding of its pathogenicity and ability to interact with other periodontal pathogens. to evaluate the virulence potential of f. alocis and its ability to interact with porphyromonas gingivalis w83, several clinical isolates of f. alocis were characterized. f. alocis showed nongingipain protease and sialidase activitie ... | 2011 | 21825062 |
| the rela/spot homolog (rsh) superfamily: distribution and functional evolution of ppgpp synthetases and hydrolases across the tree of life. | rela/spot homologue (rsh) proteins, named for their sequence similarity to the rela and spot enzymes of escherichia coli, comprise a superfamily of enzymes that synthesize and/or hydrolyze the alarmone ppgpp, activator of the "stringent" response and regulator of cellular metabolism. the classical "long" rshs rel, rela and spot with the ppgpp hydrolase, synthetase, tgs and act domain architecture have been found across diverse bacteria and plant chloroplasts, while dedicated single domain ppgpp- ... | 2011 | 21858139 |
| polymicrobial periodontal pathogen transcriptomes in calvarial bone and soft tissue. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia are consistently associated with adult periodontitis. this study sought to document the host transcriptome to a p. gingivalis, t. denticola, and t.forsythia challenge as a polymicrobial infection using a murine calvarial model of acute inflammation and bone resorption. mice were infected with p. gingivalis, t. denticola, and t. forsythia over the calvaria, after which the soft tissues and calvarial bones were excised. a muri ... | 2011 | 21896157 |
| deciphering morphological determinants of the helix-shaped leptospira. | leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiology of these pathogens. in this study, we showed that peptidoglycan sacculi from leptospira spp. retain the helical shape of intact cells. interestingly, the distribution of muropept ... | 2011 | 21926230 |
| distinct and complex bacterial profiles in human periodontitis and health revealed by 16s pyrosequencing. | periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. with advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. we used 454 sequencing of 16s rrna genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. amplicons from both the v1-2 and v4 regions of the 16s gene were sequenced, yielding 1 393 57 ... | 2011 | 22170420 |
| distinct and complex bacterial profiles in human periodontitis and health revealed by 16s pyrosequencing. | periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. with advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. we used 454 sequencing of 16s rrna genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. amplicons from both the v1-2 and v4 regions of the 16s gene were sequenced, yielding 1 393 57 ... | 2011 | 22170420 |
| Treponema denticola major outer sheath protein induces actin assembly at free barbed ends by a PIP2-dependent uncapping mechanism in fibroblasts. | The major outer sheath protein (Msp) of Treponema denticola perturbs actin dynamics in fibroblasts by inducing actin reorganization, including subcortical actin filament assembly, leading to defective calcium flux, diminished integrin engagement of collagen, and retarded cell migration. Yet, its mechanisms of action are unknown. We challenged Rat-2 fibroblasts with enriched native Msp. Msp activated the small GTPases Rac1, RhoA and Ras, but not Cdc42, yet only Rac1 localized to areas of actin re ... | 2011 | 21901132 |
| Filifactor alocis interactions with gingival epithelial cells. | An association between the gram-positive anaerobe Filifactor alocis and periodontal disease has recently emerged; however, possible pathogenic mechanisms have not been investigated. In this study we examined the responses of primary cultures of gingival epithelial cells (GECs) to infection with F. alocis. Secretion of the pro-inflammatory cytokines interleukin-1ß, interleukin-6 and tumor necrosis factor-a from GECs was stimulated by F. alocis infection. F. alocis also induced apoptosis in GECs t ... | 2011 | 22053964 |
| immune evasion strategies of porphyromonas gingivalis. | porphyromonas gingivalis is strongly correlated with chronic periodontitis. its chronic persistence in the periodontium depends on its ability to evade host immunity without inhibiting the overall inflammatory response, which is actually beneficial for this and other periodontal bacteria. indeed, the inflammatory exudate (gingival crevicular fluid) is a source of essential nutrients, such as peptides and hemin-derived iron. in this review, i discuss how p. gingivalis can promote its adaptive fit ... | 2011 | 22162663 |
| Highly Specific Protease-Based Approach for Detection of Porphyromonas gingivalis in Diagnosis of Periodontitis. | Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides that contain d-amino acids, led to the discovery of five P. gingivalis-specific substrates. Due to the presence of lysine and arginine residues in these substrates, it was hypothesize ... | 2012 | 22075590 |
| s100a2 level changes are related to human periodontitis. | periodontitis is an inflammatory disease, which, when severe, can result in tooth loss, that affects the quality of life. s100a2 was previously identified as a component of gingival crevicular fluid (gcf) via proteome analysis, but it has not been investigated whether s100a2 plays a role in periodontitis. in this study, we analyzed mrna expression of s100a2 in gingival tissues from normal and classified periodontal disease patients and compared it to that of s100a8 and s100a9. quantitative real ... | 2011 | 21922197 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| composition and localization of treponema denticola outer membrane complexes. | the treponema denticola outer membrane lipoprotein-protease complex (dentilisin) contributes to periodontal disease by degrading extracellular matrix components and disrupting intercellular host signaling pathways. we recently demonstrated that prcb, located upstream of and cotranscribed with prca and prtp, encodes a 22-kda lipoprotein that interacts with prtp and is required for its activity. here we further characterize products of the protease locus and their roles in expression, formation, a ... | 2011 | 21986628 |
| Co-expression of Arabidopsis thaliana phytochelatin synthase and Treponema denticola cysteine desulfhydrase for enhanced arsenic accumulation. | Arsenic is one of the most hazardous pollutants found in aqueous environments and has been shown to be a carcinogen. Phytochelatins (PCs), which are cysteine-rich and thio-reactive peptides, have high binding affinities for various metals including arsenic. Previously, we demonstrated that genetically engineered Saccharomyces cerevisiae strains expressing phytochelatin synthase (AtPCS) produced PCs and accumulated arsenic. In an effort to further improve the overall accumulation of arsenic, cyst ... | 2012 | 21915851 |
| pathogenic microbes and community service through manipulation of innate immunity. | the periodontal pathogen porphyromonas gingivalis undermines major components of innate immunity, such as complement, toll-like receptors (tlr), and their crosstalk pathways. at least in principle, these subversive activities could promote the adaptive fitness of the entire periodontal biofilm community. in this regard, the virulence factors responsible for complement and tlr exploitation (gingipain enzymes, atypical lipopolysaccharide molecules, and fimbriae) are released as components of readi ... | 2012 | 21948363 |
| [Role of several periodontopathogenic microorganisms and tlr4 gene Asp299Gly polymorphism in atherosclerosis pathogenesis]. | Establishment of presence of periodonto-pathogenic microorganisms in atherosclerosis plaque and surrounding tissues, and possible relation of development of atherosclerosis and TLR4 gene Asp299Gly polymorphism in ischemic heart disease patients (IHD). | 2011 | 22145357 |
| evidence for graft colonization with periodontal pathogens in lung transplant recipients. | summary bronchiolitis obliterans syndrome (bos) is a major cause of late graft dysfunction in lung transplant recipients. there is increasing evidence that beside alloimmunologic injury also non-alloimmunologic inflammatory conditions may raise the risk of acute and chronic rejection. the oral cavity represents a possible reservoir for pathogenic bacteria due to its close anatomical proximity. in this pilot study, the presence of pathogenic periodontal bacteria in the oral cavity as well as in t ... | 2011 | 22203528 |
| correlation network analysis applied to complex biofilm communities. | the complexity of the human microbiome makes it difficult to reveal organizational principles of the community and even more challenging to generate testable hypotheses. it has been suggested that in the gut microbiome species such as bacteroides thetaiotaomicron are keystone in maintaining the stability and functional adaptability of the microbial community. in this study, we investigate the interspecies associations in a complex microbial biofilm applying systems biology principles. using corr ... | 2011 | 22163302 |
| the relationship of the oral microbiotia to periodontal health and disease. | the oral microbial community represents the best-characterized consortium associated with the human host. there are strong correlations between the qualitative composition of the oral microbiota and clinically healthy or diseased states. however, additional studies are needed to elucidate the mechanisms that define these microbial/host relationships. | 2011 | 22018230 |
| detection of periodontal pathogens in newborns and children with mixed dentition. | we report the age-related prevalence of red complex periodontal pathogens, porphyromonas gingivalis, treponema denticola, and tannerella forsythia, along with four strains of orange complex pathogens. the bacteria present in samples isolated from tongue, cheek, and subgingival sulcus in edentulous newborns and children with mixed dentition were monitored by polymerase chain reaction (pcr). p. gingivalis was not detected in any site of any subject in the two groups tested. however, t. denticola w ... | 2011 | 21928086 |
| a machine-learning approach to identify hydrogenosomal proteins in trichomonas vaginalis. | the protozoan parasite trichomonas vaginalis is the causative agent of trichomoniasis, the most widespread non-viral sexually transmitted disease in humans. it possesses hydrogenosomes - anaerobic mitochondria that generate h(2), co(2), and acetate from pyruvate while converting adp to atp via substrate level phosphorylation. t. vaginalis hydrogenosomes lack a genome and translation machinery, hence they import all their proteins from the cytosol, but to date only 30 imported proteins have been ... | 2011 | 22140228 |
| Identification of iron-regulated genes of Bifidobacterium breve UCC2003 as a basis for controlled gene expression. | Iron is an essential growth factor for virtually all organisms. However, iron is not readily available in most environments and microorganisms have evolved specialized mechanisms, such as the use of siderophores and high-affinity transport systems, to acquire iron when confronted with iron-limiting conditions. In general these systems are tightly regulated to prevent iron-induced toxicity and because they are quite costly to the microbe. Because of this tight regulation we chose to explore the r ... | 2012 | 22179149 |
| the antibacterial activity of ll-37 against treponema denticola is dentilisin-protease independent and facilitated by the major outer sheath protein virulence factor. | host defense peptides are innate immunoeffectors that possess both bactericidal activities and immunomodulatory functions. deficiency in the human ll-37 host defense peptide has been previously correlated with severe periodontal disease. treponema denticola is an oral anaerobic spirochete closely associated with the pathogenesis of periodontal disease. the t. denticola major surface protein (msp), involved in adhesion and in cytotoxicity, and the dentilisin serine protease are key virulence fact ... | 2011 | 22184422 |
| relationship between periodontal diseases and preterm birth: recent epidemiological and biological data. | for ten years, the incidence of preterm birth does not decrease in developed countries despite the promotion of public health programs. many risk factors have been identified including ethnicity, age, tobacco, and infection. however, almost 50% of preterm birth causes remain unknown. the periodontal diseases are highly prevalent inflammatory and infectious diseases of tooth supporting tissues leading to an oral disability. they influence negatively general health worsening cardiovascular disease ... | 2011 | 22132334 |
| salivary levels of antibacterial peptide (ll-37/hcap-18) and cotinine in periodontitis patients. | background: the purpose of this study was to examine the relationship between salivary ll-37 levels and clinical severity in chronic periodontitis (cp) patients. the presence/absence of four periodontopathic bacteria and salivary cotinine levels were also examined in order to assess the impact of these factors on ll-37 production. methods: unstimulated salivary samples were collected from 69 cp patients. salivary concentrations of ll-37 and cotinine were measured by enzyme-linked immunosorbent ... | 2011 | 21942788 |
| characterization and scope of s-layer protein o-glycosylation in tannerella forsythia. | cell surface glycosylation is an important element in defining the life of pathogenic bacteria. tannerella forsythia is a gram-negative, anaerobic periodontal pathogen inhabiting the subgingival plaque biofilms. it is completely covered by a two-dimensional crystalline surface layer (s-layer) composed of two glycoproteins. although the s-layer has previously been shown to delay the bacterium's recognition by the innate immune system, we characterize here the s-layer protein o-glycosylation as a ... | 2011 | 21911490 |
| Porphyromonas gingivalis: a clonal pathogen?: Diversities in housekeeping genes and the major fimbriae gene. | The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been established at www.pubmlst.org/pgingivalis, and data from the database indicate a high degree of genetic diversi ... | 2011 | 22125739 |
| Protease inhibitor levels in periodontal health and disease. | Kretschmar S, Yin L, Roberts F, London R, Flemmig TT, Arushanov D, Kaiyala K, Chung WO. Protease inhibitor levels in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01425.x. © 2011 John Wiley & Sons A/S Background and Objective: Our previous study showed that protease inhibitors were attenuated by the periodontal pathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize that fewer protease inhibitors would be present in more adva ... | 2011 | 22029638 |
| Protease inhibitor levels in periodontal health and disease. | Kretschmar S, Yin L, Roberts F, London R, Flemmig TT, Arushanov D, Kaiyala K, Chung WO. Protease inhibitor levels in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01425.x. © 2011 John Wiley & Sons A/S Background and Objective: Our previous study showed that protease inhibitors were attenuated by the periodontal pathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize that fewer protease inhibitors would be present in more adva ... | 2011 | 22029638 |
| role of sialidase in glycoprotein utilization by tannerella forsythia. | the major bacterial pathogens associated with periodontitis include tannerella forsythia. we previously discovered that sialic acid stimulates biofilm growth of t. forsythia, and that sialidase activity is key to utilization of sialoconjugate sugars and is involved in host-pathogen interactions in vitro. the aim of this work was to assess the influence of the nanh sialidase on initial biofilm adhesion and growth in experiments where the only source of sialic acid was sialoglycoproteins or human ... | 2011 | 21885482 |
| Conservation of the C-type lectin fold for massive sequence variation in a Treponema diversity-generating retroelement. | Anticipatory ligand binding through massive protein sequence variation is rare in biological systems, having been observed only in the vertebrate adaptive immune response and in a phage diversity-generating retroelement (DGR). Earlier work has demonstrated that the prototypical DGR variable protein, major tropism determinant (Mtd), meets the demands of anticipatory ligand binding by novel means through the C-type lectin (CLec) fold. However, because of the low sequence identity among DGR variabl ... | 2011 | 21873231 |
| the effect of spiramycin on porphyromonas gingivalis and other "classic" periopathogens. | in clinical trials, spiramycin has shown additional benefit overscaling and root planing on pocket depth reduction, but its effect on periodontal microbiota was evaluated only by darkfield microscopy. therefore, this study was conducted to determine the effect of spiramycin administration on porphyromonas gingivalis and other periodontopathic bacteria using 16s rarn pcr technique. thirty two non-smoker adults with untreated periodontitis and pocket depth > or = 7 mm. were evaluated to participat ... | 2011 | 22010417 |
| clinical and microbiological evaluation, by real-time pcr, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole. | background: to evaluate the clinical and microbiological changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole, in individuals with aggressive periodontitis (agp). methods: 15 subjects with agp received non-surgical periodontal treatment and 45 days after completion of treatment, were treated with antibiotics. clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment and 1 mon ... | 2011 | 22060046 |
| symptomatic and asymptomatic apical periodontitis associated with red complex bacteria: clinical and microbiological evaluation. | in this study, the association of red complex (rc) bacteria that include treponema denticola, tannerella forsythia and porphyromonas gingivalis with acute, exacerbated or chronic apical periodontitis was evaluated. seventy-one patients with periapical disease were evaluated by clinical examination and microbiological samples obtained from the root canals were analyzed by a polymerase chain reaction assay. twenty-one (29.6%) samples were positive for rc bacteria, with t. ... | 2011 | 22143381 |
| tobacco smoke augments porphyromonas gingivalis-streptococcus gordonii biofilm formation. | smoking is responsible for the majority of periodontitis cases in the us and smokers are more susceptible than non-smokers to infection by the periodontal pathogen porphyromonas gingivalis. p. gingivalis colonization of the oral cavity is dependent upon its interaction with other plaque bacteria, including streptococcus gordonii. microarray analysis suggested that exposure of p. gingivalis to cigarette smoke extract (cse) increased the expression of the major fimbrial antigen (fima), but not the ... | 2011 | 22110637 |
| genetic organisation, mobility and predicted functions of genes on integrated, mobile genetic elements in sequenced strains of clostridium difficile. | clostridium difficile is the leading cause of hospital-associated diarrhoea in the us and europe. recently the incidence of c. difficile-associated disease has risen dramatically and concomitantly with the emergence of 'hypervirulent' strains associated with more severe disease and increased mortality. c. difficile contains numerous mobile genetic elements, resulting in the potential for a highly plastic genome. in the first sequenced strain, 630, there is one proven conjugative transposon (ctn) ... | 2011 | 21876735 |
| Associations of periodontal microorganisms with salivary proteins and MMP-8 in gingival crevicular fluid. | OBJECTIVE: We investigated in subjects with and without periodontitis, the levels of certain salivary proteins and matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF), in relation to the presence of specific periodontal pathogens. METHODS: Clinical parameters were recorded at baseline, in 1985 and in 2009 from 99 subjects; 56 with and 43 without periodontitis (mean age 59.2 ± SD 2.9). Saliva samples collected in 2009 were analysed for salivary albumin, total protein and immunog ... | 2011 | 22103335 |
| competitive selection of lactic acid bacteria that persist in the human oral cavity. | lactic acid bacteria (lab) might offer opportunities as oral probiotics provided candidate strains persist in the mouth. after intake of a mixture of 69 lab, strains of lactobacillus fermentum and lactobacillus salivarius were especially recovered. coaggregation with other microbes is likely not a prerequisite for persistence since l. salivarius strongly coaggregated with typical oral cavity isolates, whereas l. fermentum failed to display this phenotype. | 2011 | 21965405 |
| microbiologic findings 1 year after partial- and full-mouth scaling in the treatment of moderate chronic periodontitis. | to investigate the impact on microbiologic variables of full-mouth scaling (fms) and conventional scaling and root planing (csrp) after 12 months. | 2011 | 21909493 |
| animal models to study host-bacteria interactions involved in periodontitis. | animal models have distinct advantages because they can mimic cellular complexities that occur in humans in vivo and are often more accurate than in vitro studies that take place on plastic surfaces with limited numbers of cell types present. furthermore, cause and effect relationships can be established by applying inhibitors or activators or through the use of genetically modified animals. such gain or loss of function studies are often difficult to achieve in human clinical studies, particula ... | 2012 | 22142960 |
| variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. | objectivethe associations between oral diseases and increased risk of pancreatic cancer have been reported in several prospective cohort studies. in this study, we measured variations of salivary microbiota and evaluated their potential associations with pancreatic cancer and chronic pancreatitis.methodsthis study was divided into three phases: (1) microbial profiling using the human oral microbe identification microarray to investigate salivary microbiota variation between 10 resectable patient ... | 2011 | 21994333 |
| variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. | objectivethe associations between oral diseases and increased risk of pancreatic cancer have been reported in several prospective cohort studies. in this study, we measured variations of salivary microbiota and evaluated their potential associations with pancreatic cancer and chronic pancreatitis.methodsthis study was divided into three phases: (1) microbial profiling using the human oral microbe identification microarray to investigate salivary microbiota variation between 10 resectable patient ... | 2011 | 21994333 |
| Treatment of residual pockets with photodynamic therapy, diode laser, or deep scaling. A randomized, split-mouth controlled clinical trial. | The objective of this work was to compare the effects of antimicrobial photodynamic therapy (PDT), diode soft laser therapy (DSL), and thorough deep scaling and root planing (SRP) for treatment of residual pockets. Thirty-two subjects with a history of non-surgical treatment for chronic periodontitis were included. Residual pockets >4 mm and bleeding upon probing were debrided with an ultrasonic device and then subjected to either PDT, DSL, or SRP. Pocket probing depth (PPD), bleeding on probing ... | 2011 | 22105837 |
| human microbiome and hiv/aids. | understanding of the human microbiome continues to grow rapidly; however, reports on changes in the microbiome after hiv infection are still limited. this review surveys the progress made in methodology associated with microbiome studies and highlights the remaining challenges to this field. studies have shown that commensal oral, gut, vaginal, and penile bacteria are vital to the health of the human immune system. our studies on crosstalk among oral and gastrointestinal soluble innate factors, ... | 2011 | 22193889 |
| A New Real-Time PCR Assay for Improved Detection of the Parasite Babesia microti. | Babesiosis is an emerging zoonosis with important public health implications, as the incidence of the disease has risen dramatically over the past decade. Because the current gold standard for detection of Babesia is microscopic examination of blood smears, accurate identification requires trained personnel. Species in the genus cannot be distinguished microscopically, and Babesia can also be confused with the early trophozoite stage (ring forms) of Plasmodium parasites. To allow more accurate d ... | 2011 | 22170915 |
| colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers. | gerodontology 2011; doi: 10.1111/j.1741-2358.2011.00506.x colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers objective: the purpose of this study was to investigate colonisation by periodontopathic bacteria and the sites of colonisation in elderly upper and lower complete denture wearers. we also investigated the relationship between level of oral hygiene and colonisation by periodontopathic bacteria. materials and methods: forty edentulous and 37 dentate ... | 2011 | 21929616 |
| life cycle stage-resolved proteomic analysis of the excretome/secretome from strongyloides ratti--identification of stage-specific proteases. | a wide range of biomolecules, including proteins, are excreted and secreted from helminths and contribute to the parasite's successful establishment, survival, and reproduction in an adverse habitat. excretory and secretory proteins (esp) are active at the interface between parasite and host and comprise potential targets for intervention. the intestinal nematode strongyloides spp. exhibits an exceptional developmental plasticity in its life cycle characterized by parasitic and free-living gener ... | 2011 | 21964353 |
| Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. | Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age?=?4.6±1.2 mo old) and their mothers or primary care givers (mean age?=?30.8±9.5 y ... | 2011 | 21853142 |
| the capsule of porphyromonas gingivalis leads to a reduction in the host inflammatory response, evasion of phagocytosis, and increase in virulence. | periodontal disease is a chronic oral inflammatory disease that is triggered by bacteria such as porphyromonas gingivalis. p. gingivalis strains exhibit great heterogeneity, with some strains being encapsulated while others are nonencapsulated. although the encapsulated strains have been shown to be more virulent in a mouse abscess model, so far the role of the capsule in p. gingivalis interactions with host cells is not well understood and its role in virulence has not been defined. here, we in ... | 2011 | 21911459 |
| molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan. | periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ... | 2011 | 21916371 |
| molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard dna-dna hybridization. | knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. the purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 dna probes for different bacterial species by using the checkerboard dna-dna hybridization (cddh) technique. | 2012 | 22196182 |
| polymicrobial interactions: impact on pathogenesis and human disease. | summary: microorganisms coexist in a complex milieu of bacteria, fungi, archaea, and viruses on or within the human body, often as multifaceted polymicrobial biofilm communities at mucosal sites and on abiotic surfaces. only recently have we begun to appreciate the complicated biofilm phenotype during infection; moreover, even less is known about the interactions that occur between microorganisms during polymicrobial growth and their implications in human disease. therefore, this review focuses ... | 2012 | 22232376 |
| microbial profile of patients with periodontitis compared with healthy subjects. | objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ... | 2012 | 22257880 |
| full-mouth disinfection and systemic antimicrobial therapy in generalized aggressive periodontitis: a randomized, placebo-controlled trial. | aim: the present investigation aimed to analyse clinical and microbiological effects of systemic administration of metronidazole and amoxicillin combined with the one-stage-full-mouth-disinfection protocol (osfmd) in generalized aggressive periodontitis patients (g-agp). materials and methods: thirty-nine systemically healthy patients with g-agp were consecutively included. the test group (n = 19) received amoxicillin-metronidazole combination (500 mg of each, three times a day for 7 days) and t ... | 2012 | 22220822 |
| impact of supragingival therapy on subgingival microbial profile in smokers versus non-smokers with severe chronic periodontitis. | the aim of this study was to assess subgingival microbiological changes in smokers versus non-smokers presenting severe chronic periodontitis after supragingival periodontal therapy (st). | 2012 | 22232720 |
| necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study. | objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ... | 2012 | 22259811 |