| evidence of parietal amine oxidase activity in solanum torvum sw. stem calli after ralstonia solanacearum inoculation. | calli induced from solanum torvum stem explants were inoculated with ralstonia solanacearum under partial vacuum. all calli showed a hypersensitive response after infiltration. furthermore, amine oxidase activity with aldehyde and h(2)o(2) production was detected in semi-purified cell walls of calli infiltrated by the bacteria. due to its preferential affinity for monoamines, this enzyme is supposed to have monoamine oxidase-like (mao-like) activity. moreover, the presence of hydroxyl radicals i ... | 2009 | 19201615 |
| characterization of a novel 3-hydroxybutyrate dehydrogenase from ralstonia pickettii t1. | we previously reported that the activities of two 3-hydroxybutyrate dehydrogenases (bdh1 and bdh2) were greatly influenced by culture conditions when ralstonia pickettii t1, a strain growing on extracellular poly-3-hydroxybutyrate (phb), was grown on different carbon sources such as 3hb and succinate. in this study, knockout mutants of bdh1 or bdh2 were constructed and characterized under different culture conditions. in addition, a novel bdh (bdh3) was found in bdh2 mutants, and bdh3 was cloned ... | 2009 | 19219638 |
| a transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp. strain wbc-3. | pseudomonas sp. strain wbc-3 utilizes methyl parathion (o,o-dimethyl o-p-nitrophenol phosphorothioate) or para-nitrophenol as the sole source of carbon, nitrogen and energy. a gene encoding methyl parathion hydrolase (mph) had been characterized previously and found to be located on a typical class i composite transposon that comprised is6100 (tnmph). in this study, the transposability of this transposon was confirmed by transposition assays in two distinct mating-out systems. tnmph was demonstr ... | 2009 | 19222584 |
| chimeric enzyme composed of polyhydroxyalkanoate (pha) synthases from ralstonia eutropha and aeromonas caviae enhances production of phas in recombinant escherichia coli. | chimeric enzymes composed of polyhydroxyalkanoate (pha) synthases from ralstonia eutropha (cupriavidus necator) (phac(re)) and aeromonas caviae (phac(ac)) were constructed. phac(re) is known for its potent enzymatic activity among the characterized pha synthases. phacac has broad substrate specificity and synthesizes short-chain-length (scl)/medium-chain-length (mcl) pha. we attempted to create chimeric enzymes inheriting both of the advantageous properties. among eight chimeras, acre12, with 26 ... | 2009 | 19226108 |
| metagenomics revealed a quorum quenching lactonase qlca from yet unculturable soil bacteria. | quorum sensing (qs) is a signal mediated cell-cell communication system that couples bacterial cell density to a synchronized gene expression (fuqua et al., 1994). mostly, in gram negative bacteria qs signals are n-acylhomoserine lactones (nahls) that coordinate important functions such as virulence and pathogenicity. qs signals or the elements involved in their production or perception could be targeted to disrupt qs, a phenomenon called quorum quenching (qq). qq properties (chemicals and enzym ... | 2008 | 19226736 |
| multivalent human blood group abh and lewis glycotopes are key recognition factors for a lfuc>man binding lectin from phytopathogenic ralstonia solanacearum. | ralstonia solanacearum lectin (rsl), that might be involved in phytopathogenicity, has been defined as lfuc>>man specific. however, the effects of polyvalency of glycotopes and mammalian structural units on binding have not been established. in this study, recognition factors of rsl were comprehensively examined with natural multivalent glycotopes and monomeric ligands using enzyme linked lectin-sorbent and inhibition assays. among the glycans tested, rsl reacted strongly with multivalent blood ... | 2009 | 19233247 |
| horizontal gene transfer between ralstonia solanacearum strains detected by comparative genomic hybridization on microarrays. | the plant pathogenic betaproteobacterium ralstonia solanacearum is a complex species in that most of the strains share the common characteristic of being naturally transformable. in this study, we used a new approach based on comparative genomic hybridization (cgh) on microarrays to investigate the extent of horizontal gene transfers (hgts) between different strains of r. solanacearum. recipient strains from phylotypes i, ii and iii were naturally transformed in vitro by genomic dna from the gmi ... | 2009 | 19242532 |
| pathway and evolutionary implications of diphenylamine biodegradation by burkholderia sp. strain js667. | diphenylamine (dpa) is a common contaminant at munitions-contaminated sites as well as at aniline manufacturing sites. little is known about the biodegradation of the compound, and bacteria able to use dpa as the growth substrate have not been reported. burkholderia sp. strain js667 and ralstonia sp. strain js668 were isolated by selective enrichment from dpa-contaminated sediment. the isolates grew aerobically with dpa as the sole carbon, nitrogen, and energy source. during induction of dpa deg ... | 2009 | 19251893 |
| production of short-chain-length/medium-chain-length polyhydroxyalkanoate (pha) copolymer in the plastid of arabidopsis thaliana using an engineered 3-ketoacyl-acyl carrier protein synthase iii. | short-chain-length/medium-chain-length (scl/mcl) polyhydroxyalkanoate (pha) was produced in the plastids of arabidopsis thaliana. phe87thr (f87t) mutated 3-ketoacyl-acyl carrier protein (acp) synthase iii (fabh) from escherichia coli , and ser325thr/gln481lys (st/qk) mutated polyhydroxyalkanoate (pha) synthase (phac1) from pseudomonas sp. 61-3, along with the beta-ketothiolase (phaa) and acetoacetyl-coa reductase (phab) from ralstonia eutropha (cupriavidus necator) genes were introduced into ara ... | 2009 | 19265441 |
| use of a microchip electrophoresis system for estimation of bacterial phylogeny and analysis of no3- reducing bacterial flora in field soils. | phylogenetic estimation method without determination of dna sequence was developed. by this method, fragment length polymorphism separately digested with multiple restriction enzymes was measured using microchip electrophoresis and affiliated with those calculated from corresponding dna sequence in the theoretical database. the phylogenies of 129 no(3)(-) reducing bacteria newly isolated from field soils were estimated by this method, and were compared to those by carbon source utilization profi ... | 2009 | 19270404 |
| diverse protein regulations on pha formation in ralstonia eutropha on short chain organic acids. | organic acids are considered as potential substrates for biosynthesis of polyhydroxyalkaonates. the acids may also be the metabolic inhibitors at moderate concentration levels. in this study, ralstonia eutropha was used to elucidate the protein regulations when the bacterial cells pre-cultivated on glucose were exposed to three representative short chain organic acids, acetic, propionic and levulinic acids. the research compared and examined the proteins that might participate in pha metabolism, ... | 2009 | 19270755 |
| migratory response of soil bacteria to lyophyllum sp. strain karsten in soil microcosms. | in this study, the selection of bacteria on the basis of their migration via fungal hyphae in soil was investigated in microcosm experiments containing lyophyllum sp. strain karsten (dsm2979). one week following inoculation with a bacterial community obtained from soil, selection of a few specific bacterial types was noticed at 30 mm in the growth direction of lyophyllum sp. strain karsten in sterile soil. cultivation-based analyses showed that the migration-proficient types encompassed 10 bacte ... | 2009 | 19286795 |
| enzyme-linked immunosorbent assay detection of ralstonia solanacearum in potatoes: the south african experience. | outbreaks of bacterial wilt caused by ralstonia solanacearum in south africa in the 1980s necessitated the development of a sensitive assay for its detection. in this chapter, the development of an enzyme-linked immunosorbent assay (elisa) for the detection of ralstonia solanacearum including antibody production, the elisa method itself, potato sample preparation for testing, elisa result validation and interpretation, and confirmation of infection are discussed. data showing the drop in the ral ... | 2009 | 19301746 |
| indirect immunofluorescence microscopy for the detection and identification of plant pathogenic bacteria (in particular for ralstonla solanacearum). | immunofluorescence microscopy is a very sensitive serological test which harnesses both the power of antibodies to bind to targets along with the use of the fluorescence microscope to visualise the structures to which they bind. antibody binding is visualised by the fluorescent emission from a marker molecule bound to the antibody. the technique is of particular use in bacteriology and pathology where the location and morphology of the bacterial cells can be viewed along with the location of the ... | 2009 | 19301749 |
| spectroscopic insights into the oxygen-tolerant membrane-associated [nife] hydrogenase of ralstonia eutropha h16. | this study provides the first spectroscopic characterization of the membrane-bound oxygen-tolerant [nife] hydrogenase (mbh) from ralstonia eutropha h16 in its natural environment, the cytoplasmic membrane. the h2-converting mbh is composed of a large subunit, harboring the [nife] active site, and a small subunit, capable in coordinating one [3fe4s] and two [4fe4s] clusters. the hydrogenase dimer is electronically connected to a membrane-integral cytochrome b. epr and fourier transform infrared s ... | 2009 | 19304663 |
| enhanced defense responses in arabidopsis induced by the cell wall protein fractions from pythium oligandrum require sgt1, rar1, npr1 and jar1. | the cell wall protein fraction (cwp) is purified from the non-pathogenic biocontrol agent pythium oligandrum and is composed of two glycoproteins (pod-1 and pod-2), which are structurally similar to class iii elicitins. in tomato plants treated with cwp, jasmonic acid (ja)- and ethylene (et)-dependent signaling pathways are activated, and resistance to ralstonia solanaceraum is enhanced. to dissect cwp-induced defense mechanisms, we investigated defense gene expression and resistance to bacteria ... | 2009 | 19304739 |
| characterization of putative tryptophan monooxygenase from ralstonia solanacearum [corrected]. | the amino-acid sequence of a putative tryptophan monooxygenase (ptmo) from ralstonia solanacearum is homologous with that of proenzyme (propao) of l-phe oxidase (deaminating and decarboxylating) (pao) from pseudomonas sp. p-501 in their overall sequences. ptmo was expressed in e. coli and purified, but had no catalytic activity to oxidize l-phe. by treating ptmo with various proteases, the pronase-treated ptmo (ptmop) showed a relatively high activity to oxidize l-phe, l-trp, l-tyr and l-met. st ... | 2009 | 19304791 |
| screening of strain-specific actinobacillus pleuropneumoniae genes using a combination method. | we describe a three-step method designed to identify distinct antigen-coding genes between two related bacterial genomes by: (a) constructing a subtractive library using representational difference analysis (rda), (b) characterization of gene expression in vitro using a ribosome display system combined with antibody screening and (c) gene recovery and confirmation using rt-pcr and reverse southern hybridization, respectively. to test the efficacy of this strategy we screened the antigen-coding g ... | 2009 | 19318057 |
| mutational analysis of the inducer recognition sites of the lysr-type transcriptional regulator tfdt of burkholderia sp. nk8. | tfdt is a lysr-type transcriptional regulator that activates the transcription of the chlorocatechol degradative gene operon tfdcdef of the chlorobenzoate-degrading bacterium burkholderia sp. nk8. to identify the amino acids involved in the effector recognition by tfdt, a polymerase-chain-reaction-based random mutagenesis protocol was applied to introduce mutations into the tfdt gene. nine types of tfdt mutant bearing a single-amino-acid substitution at positions, lys-129, arg-199, val-226, val- ... | 2009 | 19319522 |
| conversion of volatile fatty acids into polyhydroxyalkanoate by ralstonia eutropha. | the aims of this study were to optimize condensed corn solubles (ccs) as a medium for growth of ralstonia eutropha and to determine the effects of individual volatile fatty acids (vfas) on polyhydroxyalkanoate (pha) production. | 2009 | 19320958 |
| the most-probable-number enumeration of dichlobenil and 2,6-dichlorobenzamide (bam) degrading microbes in finnish aquifers. | in groundwater subsurface deposits and a topsoil from five aquifers having 2,6-dichlorobenzamide (bam) in water, we determined the most-probable-number (mpn) of 2,6-dichlorobenzonitrile (dichlobenil) and metabolite bam degrading microorganisms. dichlobenil and bam were combined nitrogen sources in the mpn tubes, which were scored positive at concentrations <75% after 1 month incubation. aerobic and anaerobic microbes degrading dichlobenil and bam were common in samples in low numbers of 3.6-210 ... | 2009 | 19326247 |
| two type iii secretion system effectors from ralstonia solanacearum gmi1000 determine host-range specificity on tobacco. | the model pathogen ralstonia solanacearum gmi1000 is the causal agent of the bacterial wilt disease that attacks many solanaceous plants and other hosts but not tobacco (nicotiana spp.). we found that two type iii secretion system effector genes, avra and popp1, are limiting the host range of strain gmi1000 on at least three tobacco species (n. tabacum, n. benthamiana, and n. glutinosa). both effectors elicit the hypersensitive response (hr) on these tobacco species, although in different manner ... | 2009 | 19348572 |
| genetic and biochemical analyses of chlorobenzene degradation gene clusters in pandoraea sp. strain mcb032. | pandoraea sp. strain mcb032 was isolated as an emerging chlorobenzene degrader from a functionally stable bioreactor where species succession had occurred. in this study, two gene clusters encoding chlorobenzene metabolic functions have been cloned. within the cbs gene cluster, cbsa and cbsb are similar to the chlorobenzene dioxygenase and the cis-chlorobenzene dihydrodiol dehydrogenase in ralstonia sp. js705 and shown to transform chlorobenzene to 3-chlorocatechol. the clc gene cluster shows st ... | 2009 | 19365620 |
| outbreak of ralstonia pickettii bacteraemia in patients with haematological malignancies and haematopoietic stem cell transplant recipients. | | 2009 | 19376610 |
| ralstonia pickettii-induced ataxia in immunodeficient mice. | we report here the characterization of an asymmetric ataxia syndrome (head tilt and circling, with death in the most severe cases) demonstrated by profoundly immunodeficient mice housed at the institut curie spf facility. the immune system of the affected mice had been genetically modified so that they were deficient in both b and t cells. extensive bacteriologic, parasitic, serologic, and histopathologic analysis of the affected animals and their healthy controls led us to identify ralstonia pi ... | 2009 | 19389312 |
| new mobile genetic elements in cupriavidus metallidurans ch34, their possible roles and occurrence in other bacteria. | cupriavidus metallidurans strain ch34 is a beta-proteobacterium that thrives in low concentrations of heavy metals. the genetic determinants of resistance to heavy metals are located on its two chromosomes, and are particularly abundant in the two megaplasmids, pmol28 and pmol30. we explored the involvement of mobile genetic elements in acquiring these and others traits that might be advantageous in this strain using genome comparison of cupriavidus/ralstonia strains and related beta-proteobacte ... | 2009 | 19390985 |
| 16s rrna gene-based phylogenetic microarray for simultaneous identification of members of the genus burkholderia. | for cultivation-independent and highly parallel analysis of members of the genus burkholderia, an oligonucleotide microarray (phylochip) consisting of 131 hierarchically nested 16s rrna gene-targeted oligonucleotide probes was developed. a novel primer pair was designed for selective amplification of a 1.3 kb 16s rrna gene fragment of burkholderia species prior to microarray analysis. the diagnostic performance of the microarray for identification and differentiation of burkholderia species was ... | 2009 | 19396938 |
| impact of biocide treatments on the bacterial communities of the lascaux cave. | the lascaux cave contains a remarkable set of paintings from the upper palaeolithic. shortly after discovery in 1940, the cave was modified for public viewing and, in 2001, was invaded by a fusarium solani species complex. benzalkonium chloride was used from 2001 to 2004 to eliminate the fungal outbreak. in this study, we carried out a sampling in most of the cave halls and galleries. sequence analysis and isolation methods detected that the most abundant genera of bacteria were ralstonia and ps ... | 2009 | 19404600 |
| [comparison between agar dilution and three other methods for determining the susceptibility of 228 clinical isolates of non-fermenting gram-negative rods]. | susceptibility testing for non-fermenting gram-negative rods (nfgnr) is problematic; valid methods are needed for this purpose. | 2009 | 19406523 |
| identification of novel ralstonia solanacearum type iii effector proteins through translocation analysis of hrpb-regulated gene products. | the hrp type iii secretion system (ttss) is essential for the pathogenicity of ralstonia solanacearum on host plants. hrp ttss is a specialized secretion system that injects virulence proteins, the so-called type iii effector proteins, into plant cells. in r. solanacearum, the expression of hrp ttss-related genes is regulated by an arac-type transcriptional activator, hrpb. we have identified 30 hrpb-regulated hpx (hrpb-dependent expression) genes and three well-known hrpb-regulated genes, popa, ... | 2009 | 19406897 |
| isolation and characterization of a phorate degrading bacterium. | to study the degradation of phorate by a bacterium isolated from phorate-contaminated sites. | 2009 | 19413758 |
| a probable aculeacin a acylase from the ralstonia solanacearum gmi1000 is n-acyl-homoserine lactone acylase with quorum-quenching activity. | the infection and virulence functions of diverse plant and animal pathogens that possess quorum sensing systems are regulated by n-acylhomoserine lactones (ahls) acting as signal molecules. ahl-acylase is a quorum quenching enzyme and degrades ahls by removing the fatty acid side chain from the homoserine lactone ring of ahls. this blocks ahl accumulation and pathogenic phenotypes in quorum sensing bacteria. | 2009 | 19426552 |
| microbial production of 4-hydroxybutyrate, poly-4-hydroxybutyrate, and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by recombinant microorganisms. | 4-hydroxybutyrate (4hb) was produced by aeromonas hydrophila 4ak4, escherichia coli s17-1, or pseudomonas putida kt2442 harboring 1,3-propanediol dehydrogenase gene dhat and aldehyde dehydrogenase gene aldd from p. putida kt2442 which are capable of transforming 1,4-butanediol (1,4-bd) to 4hb. 4hb containing fermentation broth was used for production of homopolymer poly-4-hydroxybutyrate [p(4hb)] and copolymers poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [p(3hb-4hb)]. recombinant a. hydrophila ... | 2009 | 19434404 |
| biosorption equilibria of binary cd(ii) and ni(ii) systems onto saccharomyces cerevisiae and ralstonia eutropha cells: application of response surface methodology. | present study investigated the biosorption of cd(ii) and ni(ii) from aqueous solution onto saccharomyces cerevisiae and ralstonia eutropha non-living biomass. biomass inactivated by heat and pretreated by ethanol was used in determination of optimum conditions. the important process parameters, such as initial solution ph (2-8), initial ni(ii) concentration (11-42 mg/l), initial cd(ii) concentration (11-42 mg/l), and biomass dosage (0.2-4.7 g/l) were optimized using design of experiments (doe). ... | 2009 | 19443115 |
| ralstonia pickettii meningitis in a child with hydrocephalus. | | 2009 | 19449287 |
| interactions between hyphosphere-associated bacteria and the fungus cladosporium herbarum on aquatic leaf litter. | we investigated microbial interactions of aquatic bacteria associated with hyphae (the hyphosphere) of freshwater fungi on leaf litter. bacteria were isolated directly from the hyphae of fungi from sedimented leaves of a small stream in the national park "lower oder," germany. to investigate interactions, bacteria and fungi were pairwise co-cultivated on leaf-extract medium and in microcosms loaded with leaves. the performance of fungi and bacteria was monitored by measuring growth, enzyme produ ... | 2009 | 19452199 |
| virus-induced gene silencing reveals the involvement of ethylene-, salicylic acid- and mitogen-activated protein kinase-related defense pathways in the resistance of tomato to bacterial wilt. | bacterial wilt (bw), caused by ralstonia solanacearum, is a devastating vascular disease of tomato worldwide. however, information on tomato's defense mechanism against infection by this soil-borne bacterium is limited. in this study, virus-induced gene silencing (vigs) was employed to decipher signaling pathways involved in the resistance of tomato to this pathogen. defined sequence fragments derived from a group of genes known or predicted to be involved in ethylene (et) and salicylic acid (sa ... | 2009 | 19470092 |
| pathogenesis and stress related, as well as metabolic proteins are regulated in tomato stems infected with ralstonia solanacearum. | a comparative proteome analysis was initiated to systematically investigate the physiological response of tomato (solanum lycopersicum) to infection with ralstonia solanacearum, causal agent of bacterial wilt. plants of the susceptible tomato recombinant inbred line nhg3 and the resistant nhg13 were either infected or not infected with r. solanacearum and subsequently used for proteome analysis. two-dimensional isoelectric focussing/sodium dodecyl-sulphate polyacrylamide gel electrophoresis (2-d ... | 2009 | 19482482 |
| specific and sensitive detection of ralstonia solanacearum in soil with quantitative, real-time pcr assays. | the aim of this study was to develop a sensitive and an effective method suitable for large-scale detection and quantification of ralstonia solanacearum in soil. | 2009 | 19486215 |
| dissection of bacterial wilt on medicago truncatula revealed two type iii secretion system effectors acting on root infection process and disease development. | ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease, which colonizes susceptible medicago truncatula via the intact root tip. infection involves four steps: appearance of root tip symptoms, root tip cortical cell invasion, vessel colonization, and foliar wilting. we examined this pathosystem by in vitro inoculation of intact roots of susceptible or resistant m. truncatula with the pathogenic strain gmi1000. the infection process was type iii secretion system depe ... | 2009 | 19493968 |
| an alternative succinate (2-oxoglutarate) transport system in rhizobium tropici is induced in nodules of phaseolus vulgaris. | the rhizobial dcta permease is essential for the development of effective nitrogen-fixing bacteroids, which was correlated with its requirement for growth on c(4)-dicarboxylates. a previously described dcta mutant of rhizobium tropici ciat899, strain ga1 (dcta), however, was unexpectedly still able to grow on succinate as a sole carbon source but less efficiently than ciat899. like other rhizobial dcta mutants, ga1 was unable to grow on fumarate or malate as a carbon source and induced the forma ... | 2009 | 19502401 |
| stable expression of the chlorocatechol dioxygenase gene from ralstonia eutropha nh9 in hybrid poplar cells. | the cbna gene encoding chlorocatechol dioxygenase from the soil bacterium ralstonia eutropha nh9 under the control of a modified cauliflower mosaic virus 35s promoter was introduced into a hybrid poplar (populus tremula x p. tremuloides). integration of the cbna gene in transgenic poplar was confirmed by pcr and genomic southern blot analysis. expression of the cbna gene was analyzed by western blot analysis. transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muco ... | 2009 | 19502754 |
| rrs1 and rps4 provide a dual resistance-gene system against fungal and bacterial pathogens. | colletotrichum higginsianum is a fungal pathogen that infects a wide variety of cruciferous plants, causing important crop losses. we have used map-based cloning and natural variation analysis of 19 arabidopsis ecotypes to identify a dominant resistance locus against c. higginsianum. this locus named rch2 (for recognition of c. higginsianum) maps in an extensive cluster of disease-resistance loci known as mrc-j in the arabidopsis ecotype ws-0. by analyzing natural variations within the mrc-j reg ... | 2009 | 19519800 |
| ralstonia solanacearum encounters an oxidative environment during tomato infection. | ralstonia solanacearum genes that are induced during tomato infection suggested that this pathogen encounters reactive oxygen species (ros) during bacterial wilt pathogenesis. the genomes of r. solanacearum contain multiple redundant ros-scavenging enzymes, indirect evidence that this pathogen experiences intense oxidative stress during its life cycle. over 9% of the bacterium's plant-induced genes were also upregulated by hydrogen peroxide in culture, suggesting that oxidative stress may be lin ... | 2009 | 19522559 |
| comparative proteomic analysis of bacterial wilt susceptible and resistant tomato cultivars. | to investigate the molecular mechanisms of bacterial resistance in susceptible and resistant cultivars of tomato, a proteomic approach was adopted. four cultivars of tomato were selected on the basis of their response to bacterial (pseudomonas solanacearum) inoculation wherein cultivar roma and riogarande, and cultivar pusa ruby and pant bahr were considered as resistant and susceptible cultivars, respectively. proteins were extracted from leaves of 3-week-old seedlings of the four cultivars and ... | 2009 | 19524626 |
| mechanistic model for the reclamation of industrial wastewaters using algal-bacterial photobioreactors. | a mechanistic model describing the steady-state biodegradation of inhibitory pollutants by algal-bacterial consortia in enclosed chemostat photobioreactors was developed. the modelwasthenvalidated againstexperimentaldataonsalicylate removal by a chlorella sorokiniana/ralstonia basilensis consortium cultivated without external o2 supplied in an enclosed chemostat photobioreactor under various conditions of photon flux radiation, salicylate inlet concentrations, temperatures, and hydraulic retenti ... | 2009 | 19534135 |
| novel denitrifying bacterium ochrobactrum anthropi yd50.2 tolerates high levels of reactive nitrogen oxides. | most studies of bacterial denitrification have used nitrate (no3-) as the first electron acceptor, whereas relatively less is understood about nitrite (no2-) denitrification. we isolated novel bacteria that proliferated in the presence of high levels of no2- (72 mm). strain yd50.2, among several isolates, was taxonomically positioned within the alpha subclass of proteobacteria and identified as ochrobactrum anthropi yd50.2. this strain denitrified no2-, as well as no3-. the gene clusters for den ... | 2009 | 19542343 |
| altered composition of ralstonia eutropha poly(hydroxyalkanoate) through expression of pha synthase from allochromatium vinosum atcc 35206. | the class iii poly(hydroxyalkanoate) synthase (phas) genes (phac and phae) of a photosynthetic bacterium, allochromatium vinosum atcc 35206, were cloned, sequenced and expressed in a heterologous host. pcr coupled with a chromosomal gene-walking method was used to clone and subsequently sequence the contiguous phac (1,068 bps) and phae (1,065 bps) genes of a. vinosum atcc 35206. blastp search of protein databases showed that the gene-products of phac and phae are different (<66% identities) from ... | 2009 | 19557308 |
| comparative large-scale analysis of interactions between several crop species and the effector repertoires from multiple pathovars of pseudomonas and ralstonia. | bacterial plant pathogens manipulate their hosts by injection of numerous effector proteins into host cells via type iii secretion systems. recognition of these effectors by the host plant leads to the induction of a defense reaction that often culminates in a hypersensitive response manifested as cell death. genes encoding effector proteins can be exchanged between different strains of bacteria via horizontal transfer, and often individual strains are capable of infecting multiple hosts. host p ... | 2009 | 19571308 |
| blocking of pseudomonas aeruginosa and ralstonia solanacearum lectins by plant and microbial branched polysaccharides used as food additives. | pseudomonas aeruginosa antibiotic resistance prompted the search for glycodecoys that would block its lectin-dependent adhesion to human cells. we have used the lectins of this pathogen, pa-il (galactophilic leca) and pa-iil (fucophilic lecb), and two additional pathogenic bacterial lectins, cv-iil (fucophilic, of chromobacterium violaceum) and rs-iil (mannophilic, of ralstonia solanacearum), for assaying the pathogenic lectin-blocking abilities of some plant and microbial polysaccharidic food a ... | 2009 | 19572540 |
| metagenomics reveals diversity and abundance of meta-cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air-sparging bioremediation. | the extradiol dioxygenase diversity of a site highly contaminated with aliphatic and aromatic hydrocarbons under air-sparging treatment was assessed by functional screening of a fosmid library in escherichia coli with catechol as substrate. the 235 positive clones from inserts of dna extracted from contaminated soil were equivalent to one extradiol dioxygenase-encoding gene per 3.6 mb of dna screened, indicating a strong selection for genes encoding this function. three subfamilies were identifi ... | 2009 | 19575758 |
| tolerance of the ralstonia eutropha class i polyhydroxyalkanoate synthase for translational fusions to its c terminus reveals a new mode of functional display. | here, the class i polyhydroxyalkanoate synthase (phac) from ralstonia eutropha was investigated regarding the functionality of its conserved c-terminal region and its ability to tolerate translational fusions to its c terminus. male, the maltose binding protein, and green fluorescent protein (gfp) were considered reporter proteins to be translationally fused to the c terminus. interestingly, phac remained active only when a linker was inserted between phac and male, whereas male was not function ... | 2009 | 19581473 |
| overexpression of the soybean gmerf3 gene, an ap2/erf type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco. | a new member of the ap2/erf transcription factor family, gmerf3, was isolated from soybean. sequence analysis showed that gmerf3 contained an ap2/erf domain of 58 amino acids and two putative nuclear localization signal (nls) domains. it belonged to a group iv protein in the erf (ethylene response factor) subfamily as typified by a conserved n-terminal motif [mcggai(i/l)]. expression of gmerf3 was induced by treatments with high salinity, drought, abscisic acid (aba), salicylic acid (sa), jasmon ... | 2009 | 19602544 |
| highly sensitive and selective gold(i) recognition by a metalloregulator in ralstonia metallidurans. | a merr family metalloregulatory protein cupr selectively responds to gold stress in ralstonia metallidurans. a distorted trigonal geometry appears to be used by cupr to achieve the highly sensitive (k(d) approximately 10(-35) m) and selective recognition of gold(i). | 2009 | 19606897 |
| [acute bacterial prostatitis by ralstonia pickettii: clinical and epidemiological considerations of excepcional observation]. | | 2009 | 19615483 |
| investigation of shell disease in map turtles (graptemys spp.). | nineteen map turtles (graptemys spp.) maintained under natural conditions were investigated because of chronic shell abnormalities. animals were evaluated using a novel shell scoring system that divided the 54 scutes into six regions, with each region scored for lesion extent and severity, and summated to produce a total shell disease score (tsds). complete blood counts and various biochemistry analytes (total protein, albumin, globulin, urea, uric acid, 25-hydroxycholecalciferol, phosphorus, an ... | 2009 | 19617474 |
| [a community acquired pneumonia case caused by ralstonia pickettii]. | ralstonia pickettii, formerly known as burkholderia pickettii, is a non-fermentative gram-negative bacillus. it is emerging as an opportunistic pathogen both in the hospital setting and in the environment, leading to outbreaks especially in the intensive care units. the available literature revealed two case reports of pneumonia associated with r. pickettii in adults. in this report, a case of pneumoniae due to r. pickettii, in a patient with chronic obstructive pulmonary disease was presented. ... | 2009 | 19621622 |
| homotaurine metabolized to 3-sulfopropanoate in cupriavidus necator h16: enzymes and genes in a patchwork pathway. | homotaurine (3-aminopropanesulfonate), a natural product and an analogue of gaba (4-aminobutyrate), was found to be a sole source of nitrogen for cupriavidus necator (ralstonia eutropha) h16, whose genome sequence is known. homotaurine nitrogen was assimilated into cell material, and the quantitative fate of the organosulfonate was sulfopropanoate, which was recovered in the growth medium. the first scalar reaction was shown to be inducible homotaurine:2-oxoglutarate aminotransferase, which rele ... | 2009 | 19648235 |
| [ralstonia picketti in chronic obstructive pulmonary disease exacerbation]. | | 2010 | 19656611 |
| genetic diversity and host range variation of ralstonia solanacearum strains entering north america. | each year, large volumes of ornamental and food plant propagative stock are imported into the north america; occasionally, ralstonia solanacearum is found systemically infecting this plant material. in this study, 107 new r. solanacearum strains were collected over a 10-year period from imported propagative stock and compared with 32 previously characterized r. solanacearum strains using repetitive polymerase chain reaction (rep-pcr) element (box, eric, and rep) primers. additional strain compar ... | 2009 | 19671009 |
| specific genes from the potato brown rot strains of ralstonia solanacearum and their potential use for strain detection. | ralstonia solanacearum is the agent of bacterial wilt infecting >200 different plant species covering >50 botanical families. the genus r. solanacearum can be classified into four phylotypes and each phylotype can be further subdivided into sequevars. the potato brown rot strains of r. solanacearum from phylotype iib, sequevar 1 (iib1), historically known as race 3, biovar 2 strains, are responsible for important economic losses to the potato industry and threaten ornamental crop production worl ... | 2009 | 19671014 |
| essential role of the hprk gene in ralstonia eutropha h16. | ralstonia eutropha h16 possesses an incomplete phosphoenolpyruvate (pep):sugar phosphotransferase system (pts) composed of ei, hpr, eiia(ntr) (ptsn) and eiia(man) (ptsm). we could show that in vitro the incomplete pts phosphorylation cascade is partially functional. hpr becomes phosphorylated by pep and ei, and transfers the phosphoryl group to eiia(ntr), but only extremely slowly to eiia(man). components of this system have previously been shown to regulate the metabolism of polyhydroxybutyrate ... | 2009 | 19672046 |
| identification, biosynthesis, and characterization of polyhydroxyalkanoate copolymer consisting of 3-hydroxybutyrate and 3-hydroxy-4-methylvalerate. | in this study, we found that ralstonia eutropha strain phb(-)4 expressing the polyhydroxyalkanoate (pha) synthase 1 (phac1(ps)) from pseudomonas sp. 61-3 synthesized a pha copolymer containing a 3-hydroxybutyrate (3hb) and small amounts of 3-hydroxy-4-methylvalerate (3h4mv) and 3-hydroxyvalerate (3hv) from fructose as a sole carbon source. 3h4mv is a monomer unit that has hitherto not been reported as a naturally occurring component of phas. to increase the 3h4mv fraction in pha copolymers, the ... | 2009 | 19681605 |
| construction of phb and phbv multiple-gene vectors driven by an oil palm leaf-specific promoter. | one of the targets in oil palm genetic engineering programme is the production of polyhydroxybutyrate (phb) and polyhydroxybutyrate-co-valerate (phbv) in the oil palm leaf tissues. production of phb requires the use of phba (beta-ketothiolase type a), phbb (acetoacetyl-coa reductase) and phbc (phb synthase) genes of ralstonia eutropha, whereas bktb (beta-ketothiolase type b), phbb, phbc genes of r. eutropha and tdcb (threonine dehydratase) gene of escherichia coli were used for phbv production. ... | 2009 | 19699761 |
| expression of tfda genes in aquatic microbial communities during acclimation to 2,4-dichlorophenoxyacetic acid. | the role of gene expression during acclimation of aquatic microbial communities was examined by relating transcription of tfda to the degradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d). the tfda gene encodes for a 2,4-d/2-oxoglutarate dioxygenase that transforms 2,4-d to 2,4-dichlorophenol. transcription of tfda, the abundance of tfda genes and 2,4-d degrading populations, and the rate of 2,4-d disappearance were followed in laboratory incubations of two pond water samples that ... | 2002 | 19709228 |
| interactions of earthworms with indigenous and bioaugmented pcb-degrading bacteria. | partial bioremediation of polychlorinated biphenyl (pcb)-contaminated soils has been achieved using bioaugmentation with pcb-degrading bacteria and earthworms. to further study the contribution of earthworms to bioremediation, an experiment was conducted in which the changes in indigenous and bioaugmented pcb-degrading bacteria were analyzed during treatment of contaminated soil using earthworms (pheretima hawayana) alone or in combination with the pcb-degrading bacteria, ralstonia eutrophus and ... | 2002 | 19709253 |
| horizontal transfer of dehalogenase genes on incp1beta plasmids during bacterial adaptation to degrade alpha-halocarboxylic acids. | the diversity of bacterial alpha-halocarboxylic acid (alphaha) dehalogenases from a polluted soil was investigated. polymerase chain reaction (pcr) primers designed to amplify group i and group ii dehalogenase (deh) gene sequences were used to screen bacterial isolates, nine beta-proteobacteria and one gamma-proteobacterium, from soil enrichments. primers successfully amplified deh sequences from all 10 alphaha-utilising isolates. bacteria isolated at 15 or 30 degrees c on chloroacetic acid or 2 ... | 2003 | 19719596 |
| degradation of mixtures of aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbon-degrading bacteria. | the well-characterized toluene-oxidizing bacteria pseudomonas putida paw1, p. putida f1, p. mendocina kr1, burkholderia cepacia g4, b. cepacia js150, and ralstonia pickettii pko1, as well as several strains of bacteria isolated from activated sludge, were examined for their ability to degrade mixtures of aromatic and chloroaliphatic hydrocarbons. collectively, the strains tested were able to transform all of the 14 aromatic hydrocarbons included in the mixtures, with most strains degrading five ... | 2003 | 19719688 |
| effects of compost addition and simulated solarisation on the fate of ralstonia solanacearum biovar 2 and indigenous bacteria in soil. | abstract the effects of compost addition and simulated solarisation of soil on the survival of ralstonia solanacearum biovar 2 strain 1609, as well as on the structure of indigenous soil bacterial communities, were analysed. in addition, effects on the invasion of susceptible test plants by strain 1609 were assessed. in untreated soil in microcosms and the field, strain 1609 showed slow progressive declines, from 10(6)-10(7) to roughly 10(4)-10(5) cfu per g dry soil in around 60 days. when these ... | 2003 | 19719697 |
| interactions with hosts at cool temperatures, not cold tolerance, explain the unique epidemiology of ralstonia solanacearum race 3 biovar 2. | most strains of the bacterial wilt pathogen ralstonia solanacearum are tropical, but race 3 biovar 2 (r3bv2) strains can attack plants in temperate zones and tropical highlands. the basis of this distinctive ecological trait is not understood. we compared the survival of tropical, r3bv2, and warm-temperate north american strains of r. solanacearum under different conditions. in water at 4 degrees c, north american strains remained culturable the longest (up to 90 days), whereas tropical strains ... | 2009 | 19740025 |
| defense mechanisms of solanum tuberosum l. in response to attack by plant-pathogenic bacteria. | the natural resistance of plants to disease is based not only on preformed mechanisms, but also on induced mechanisms. the defense mechanisms present in resistant plants may also be found in susceptible ones. this study attempted to analyze the metabolic alterations in plants of the potato solanum tuberosum l. cv. agata that were inoculated with the incompatible plant-pathogenic bacteria x. axonopodis and r. solanacearum, and the compatible bacterium e. carotovora. levels of total phenolic compo ... | 2009 | 19746266 |
| influence of dna conformation and role of coma and reca on natural transformation in ralstonia solanacearum. | naturally competent bacteria such as the plant pathogen ralstonia solanacearum are characterized by their ability to take up free dna from their surroundings. in this study, we investigated the efficiency of various dna types including chromosomal linear dna and circular or linearized integrative and (or) replicative plasmids to naturally transform r. solanacearum. to study the respective regulatory role of dna transport and maintenance in the definite acquisition of new dna by bacteria, the nat ... | 2009 | 19767847 |
| multiple displacement amplification as a pre-polymerase chain reaction (pre-pcr) to detect ultra low population of ralstonia solanacearum (smith 1896) yabuchi et al. (1996). | to develop a reliable and sensitive protocol for detection of ralstonia solanacearum using mda-pcr (multiple displacement amplification-pcr amplification). | 2009 | 19780955 |
| microbial utilization of the industrial wastewater pollutants 2-ethylhexylthioglycolic acid and iso-octylthioglycolic acid by aerobic gram-negative bacteria. | industrial wastewater from the production of sulfur containing esters and the resulting products of this synthesis, 2-ethylhexylthioglycolic acid (ehtg) and iso-octylthioglycolic acid (iotg), were deployed in this study to enrich novel bacterial strains, since no wastewater and ehtg or iotg degrading microorganisms were hitherto described or available. in addition, nothing is known about the biodegradation of these thiochemicals. the effect of this specific wastewater on the growth behaviour of ... | 2010 | 19789984 |
| a proteomic view of the facultatively chemolithoautotrophic lifestyle of ralstonia eutropha h16. | ralstonia eutropha h16 is an h(2)-oxidizing, facultative chemolithoautotroph. using 2-de in conjunction with peptide mass spectrometry we have cataloged the soluble proteins of this bacterium during growth on different substrates: (i) h(2) and co(2), (ii) succinate and (iii) glycerol. the first and second conditions represent purely lithoautotrophic and purely organoheterotrophic nutrition, respectively. the third growth regime permits formation of the h(2)-oxidizing and co(2)-fixing systems con ... | 2009 | 19798673 |
| characterization of regulatory element rp3 of regulation of beta-lactamases from ralstonia pickettii. | the two chromosomally encoded beta-lactamases, oxa-22 and oxa-60, from ralstonia pickettii are inducible by beta-lactam molecules. disruption of rp3 abolished induction of both beta-lactamases and the resistance to ph, osmolarity and survival in the stationary phase, suggesting that rp3 might be a global regulator. interactions between rp3, oxa-22 and oxa-60 were investigated at a transcript and protein level using 5'-rapid amplification of cdna ends experiments, real-time reverse transcription ... | 2009 | 19817868 |
| degradation of nitroaromatic compounds: a model to study evolution of metabolic pathways. | although many nitroaromatic compounds have been in nature for only a few decades, bacteria have already evolved the ability to metabolize them. both horizontal transfer of genes and mutagenesis induced under stressful conditions might facilitate evolution of new catabolic pathways. nitrotoluene degradation pathways are supposedly derived from an ancestral naphthalene degradation pathway. the 2-nitrotoluene degradation genes in acidovorax sp. strain js42 are controlled by the transcriptional acti ... | 2009 | 19818019 |
| a dual resistance gene system prevents infection by three distinct pathogens. | colletotrichum higginsianum causes typical anthracnose lesions on the leaves, petioles, and stems of cruciferous plants. inoculation of arabidopsis thaliana ecotype columbia leaves with c. higginsianum results in fungal growth and disease symptoms reminiscent of those induced in other cruciferous plants. we performed map-based cloning and natural variation analysis of 19 a. thaliana ecotypes to identify a dominant resistance locus against c. higginsianum. we found that the a. thaliana rch2 (for ... | 2009 | 19826224 |
| characterization of a nitroreductase with selective nitroreduction properties in the food and intestinal lactic acid bacterium lactobacillus plantarum wcfs1. | nitroreductases reduce nitroaromatic compounds and other oxidants in living organisms, having interesting implications in environmental and human health. a putative nitrobenzoate reductase encoding gene (lp_0050) was recently annotated in the completed dna sequence of lactic acid bacterium lactobacillus plantarum wcfs1 strain. in this research, this l. plantarum gene was cloned and expressed, and the corresponding protein (pnba) was biochemically characterized. this l. plantarum pnba reductase i ... | 2009 | 19827797 |
| an immunocompetent patient presenting with severe septic arthritis due to ralstonia pickettii identified by molecular-based assays: a case report. | ralstonia pickettii is an infrequent pathogen of invasive infections in healthy individuals. the microorganism is supposed to be of relatively low virulence, but can cause infections, mainly of the respiratory tract, in immunocompromised and cystic fibrosis patients. ralstonia pickettii has also been associated with hospital outbreaks related to contamination of products used for medical care and laboratory diagnosis. | 2009 | 19830053 |
| the global virulence regulators vsrad and phca control secondary metabolism in the plant pathogen ralstonia solanacearum. | | 2009 | 19830761 |
| biodegradation of thiocyanate using co-culture of klebsiella pneumoniae and ralstonia sp. | thiocyanate-degrading microbial co-culture was isolated from thiocyanate-contaminated site and tested for thiocyanate degradation potential and thiocyanate-toxicity tolerance and identified as klebsiella pneumoniae and ralstonia sp. by 16s rdna sequencing. the co-culture was able to degrade thiocyanate with degradation rate of 500 mg l(-1)d(-1) at 2,500 mg l(-1) thiocyanate concentration at ph 6.0 and 37 degrees c following thiocyanate hydrolase pathway. the haldane kinetic model elucidates the ... | 2010 | 19838695 |
| reconstructing the evolutionary history of nitrotoluene detection in the transcriptional regulator ntdr. | many toxic man-made compounds have been introduced into the environment, and bacterial strains that are able to grow on them are ideal model systems for studying the evolution of metabolic pathways and regulatory systems. acidovorax sp. strain js42 is unique in its ability to use 2-nitrotoluene as a sole carbon, nitrogen, and energy source for growth. the lysr-type transcriptional regulator ntdr activates expression of the 2-nitrotoluene degradation genes not only when nitroaromatic compounds ar ... | 2009 | 19849778 |
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| stable isotope metabolic labeling with a novel n-enriched bacteria diet for improved proteomic analyses of mouse models for psychopathologies. | the identification of differentially regulated proteins in animal models of psychiatric diseases is essential for a comprehensive analysis of associated psychopathological processes. mass spectrometry is the most relevant method for analyzing differences in protein expression of tissue and body fluid proteomes. however, standardization of sample handling and sample-to-sample variability are problematic. stable isotope metabolic labeling of a proteome represents the gold standard for quantitative ... | 2009 | 19915716 |
| conversion of glycerol to poly(3-hydroxypropionate) in recombinant escherichia coli. | we have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3hp)]. for this, the genes for glycerol dehydratase (dhab1) of clostridium butyricum, propionaldehyde dehydrogenase (pdup) of salmonella enterica serovar typhimurium lt2, and polyhydroxyalkanoate (pha) synthase (phac1) of ralstonia eutropha were expressed in recombinant escherichia coli. poly(3hp) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. the present ... | 2010 | 19933347 |
| a kinetic and thermodynamic understanding of o2 tolerance in [nife]-hydrogenases. | in biology, rapid oxidation and evolution of h(2) is catalyzed by metalloenzymes known as hydrogenases. these enzymes have unusual active sites, consisting of iron complexed by carbonyl, cyanide, and thiolate ligands, often together with nickel, and are typically inhibited or irreversibly damaged by o(2). the knallgas bacterium ralstonia eutropha h16 (re) uses h(2) as an energy source with o(2) as a terminal electron acceptor, and its membrane-bound uptake [nife]-hydrogenase (mbh) is an importan ... | 2009 | 19934053 |
| acrylic acid removal from synthetic wastewater and industrial wastewater using ralstonia solanacearum and acidovorax avenae isolated from a wastewater treatment system manufactured with polyacrylonitrile fiber. | ralstonia solanacearum and acidovorax avenae were isolated from a wastewater treatment system manufactured with polyacrylonitrile fiber. the investigation goal is to elucidate the effectiveness of ralstonia solanacearum and acidovorax avenae in treating acrylic acid from synthetic wastewater and industrial wastewater. the results reveal that ralstonia solanacearum and acidovorax avenae could utilize acrylic acid from synthetic wastewater for growth, when the initial acrylic acid concentration wa ... | 2009 | 19934523 |
| [biologic properties of ralstonia solanacearum]. | two methods of estimation of potato cultivars for resistance to the agent of brown bacterial rot (ralstonia solanacearum) were used. the distribution of the most widespread agricultural zoned potato cultivars into groups depending on the degree of their resistance to bacterial quarantine agent, potentially dangerous for ukraine, can be used in the selection of the potato varieties resistant to the agent, and in the development of integrated plant protection systems. r. solanacearum was not detec ... | 2009 | 19938594 |
| novel tn4371-ice like element in ralstonia pickettii and genome mining for comparative elements. | integrative conjugative elements (ices) are important factors in the plasticity of microbial genomes. an element related to the ice tn4371 was discovered during a bioinformatic search of the ralstonia pickettii 12j genome. this element was analysed and further searches carried out for additional elements.a pcr method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight ralstonia pickettii and ralstonia insidiosa strains ... | 2009 | 19941653 |
| enhanced polyhydroxybutyrate (phb) production via the coexpressed phacab and vgb genes controlled by arabinose p promoter in escherichia coli. | to develop an approach to enhance polyhydroxybutyrate (phb) production via the coexpressed phacab and vgb genes controlled by arabinose p(bad) promoter in escherichia coli. | 2010 | 19943886 |
| genetic and phenotypic diversity of ralstonia solanacearum biovar 2 strains obtained from dutch waterways. | a novel set of ralstonia solanacearum biovar 2 isolates was obtained, at several sampling occasions, from dutch waterways, sediment and bittersweet plants and their genetic and phenotypic diversity was investigated. as reference strains, two previously-described strains obtained from diseased potato plants, denoted 1609 (the netherlands) and 715 (bangladesh), were included in the analyses. all novel isolates showed box and gtg5 pcr based genomic profiles similar to those of the reference strains ... | 2010 | 19960250 |
| expression of a synthesized gene encoding cationic peptide cecropin b in transgenic tomato plants protects against bacterial diseases. | the cationic lytic peptide cecropin b (cb), isolated from the giant silk moth (hyalophora cecropia), has been shown to effectively eliminate gram-negative and some gram-positive bacteria. in this study, the effects of chemically synthesized cb on plant pathogens were investigated. the s(50)s (the peptide concentrations causing 50% survival of a pathogenic bacterium) of cb against two major pathogens of the tomato, ralstonia solanacearum and xanthomonas campestris pv. vesicatoria, were 529.6 micr ... | 2010 | 19966019 |
| induction of immune response to the 17 kda ompa burkholderia cenocepacia polypeptide and protection against pulmonary infection in mice after nasal vaccination with an omp nanoemulsion-based vaccine. | burkholderia cepacia complex (bcc) are opportunistic bacteria associated with life-threatening illness in persons with cystic fibrosis. once bcc colonization is established, these antimicrobial-resistant and biofilm-forming bacteria are difficult to eradicate and are associated with increased rates of morbidity and mortality. at present, no vaccines are available to prevent the bcc infection. there is currently a paucity of published information regarding the development of vaccines designed to ... | 2010 | 19967396 |
| unravelling the c3/c4 carbon metabolism in ralstonia eutropha h16. | detailed knowledge about the enzymes responsible for conversion of c(3) and c(4) compounds will be helpful to establish the bacterial strain ralstonia eutropha as platform for the production of biotechnologically interesting compounds. although various studies about these enzymes were accomplished in the past, some contradicting information about the enzyme pattern in this bacterium still exists. to resolve these discrepancies, the c(3) /c(4) metabolism was reinvestigated after the genome sequen ... | 2010 | 20002867 |
| prhg, a transcriptional regulator responding to growth conditions, is involved in the control of the type iii secretion system regulon in ralstonia solanacearum. | the ability of ralstonia solanacearum to cause disease in plants depends on its type iii secretion system (t3ss). the expression of the t3ss and its effector substrates is coordinately controlled by a regulatory cascade, at the bottom of which is hrpb. transcription of the hrpb gene is activated by a plant-responsive regulator named hrpg, which is a master regulator of a wide array of pathogenicity functions in r. solanacearum. we have identified in the genome of strain gmi1000 a close paralog o ... | 2010 | 20008073 |
| a jumbo phage infecting the phytopathogen ralstonia solanacearum defines a new lineage of the myoviridae family. | phirsl1 is a jumbo myovirus stably and lytically infecting the phytopathogenic bacterium ralstonia solanacearum. in this study, we investigate the infection cycle of varphirsl1 and provide a genomic, proteomic and transcriptomic view of this phage. its 231-kbp genome sequence showed many genes lacking detectable homologs in the current databases and was vastly different from previously studied phage genomes. in addition to these orphan proteins, varphirsl1 was found to encode several enzymes tha ... | 2010 | 20034649 |
| epr and mössbauer spectroscopy show inequivalent hemes in tryptophan dioxygenase. | tryptophan 2,3-dioxygenase (tdo) is an essential enzyme in the pathway of nad biosynthesis and important for all living organisms. tdo catalyzes oxidative cleavage of the indole ring of l-tryptophan (l-trp), converting it to n-formylkynurenine (nfk). the crystal structure of tdo shows a dimer of dimer quaternary structure of the homotetrameric protein. the four catalytic sites of the protein, one per subunit, contain a heme that catalyzes the activation and insertion of dioxygen into l-trp. beca ... | 2010 | 20047315 |
| degradation and adsorption characteristics of phb depolymerase as revealed by kinetics of mutant enzymes with amino acid substitution in substrate-binding domain. | extracelluar poly[(r)-3-hydroxybutyrate] (phb) depolymerase (phaz(rpit1)) from ralstonia pickettii t1 adsorbs to phb surface via its substrate-binding domain (sbd) to enhance phb degradation. our previous study combining pcr random mutagenesis with the determination of phb degradation levels of mutant enzymes suggested that ser, tyr, val, ala, and leu residues in sbd are probably involved in the enzymatic adsorption to and degradation of phb. in the present study, the effects of mutations at leu ... | 2010 | 20058938 |
| structural studies on the full-length lysr-type regulator tsar from comamonas testosteroni t-2 reveal a novel open conformation of the tetrameric lttr fold. | lysr-type transcriptional regulators (lttrs) constitute the largest family of regulators in prokaryotes. the full-length structures of the lttr tsar from comamonas testosteroni t-2 and its complex with the natural inducer para-toluensulfonate have been characterized by x-ray diffraction. both ligand-free and complexed forms reveal a dramatically different quaternary structure from that of cbnr from ralstonia eutropha, or a putative lysr-type regulator from pseudomonas aeruginosa, the only other ... | 2010 | 20059681 |