| zoonotic transmission of reassortant porcine g4p[6] rotaviruses in hungarian pediatric patients identified sporadically over a 15 year period. | genotype g4p[6] rotavirus a (rva) strains collected from children admitted to hospital with gastroenteritis over a 15 year period in the pre rotavirus vaccine era in hungary were characterized in this study. whole genome sequencing and phylogenetic analysis was performed on eight g4p[6] rva strains. all these rva strains shared a fairly conservative genomic configuration (g4-p[6]-i1/i5-r1-c1-m1-a1/a8-n1-t1/t7-e1-h1) and showed striking similarities to porcine and porcine-derived human rva strain ... | 2013 | 23792183 |
| [genetic diversity of rotavirus group a: correlation between g3 type and severity of the infection. valencia, venezuela]. | genetic and antigenic rotavirus (rv) variabilities may have implications in the severity of the infection caused by these agents; however the studies are not conclusive. for that purpose, the mean severity scores of diarrhea episodes caused by rv types g1 and g3 were compared, at ciudad hospitalaria "dr. enrique tejera" in valencia, venezuela, between 2001-2005. rv were identified by elisa, g and p types by rt-pcr. the severity of infection was determined using the ruuska-vesikari system and the ... | 2013 | 23781711 |
| rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative pcr. | reverse transcription-real-time polymerase chain reaction (rt-qpcr) for the vp6 gene was used to study group a rotavirus shedding in children with symptomatic and asymptomatic rotavirus infection. sequential stool samples (n = 345) from 10 children with rotavirus associated diarrhea and from five children (n = 161) with asymptomatic rotavirus infection were collected over a period of 2 months. a rt-qpcr assay on the samples using a rotavirus vp6 plasmid standard demonstrated high reproducibility ... | 2013 | 23775335 |
| human g9p[8] rotavirus strains circulating in cameroon, 1999-2000: genetic relationships with other g9 strains and detection of a new g9 subtype. | group a rotaviruses (rv-a) are the leading cause of viral gastroenteritis in children worldwide and genotype g9p[8] is one of the five most common genotypes detected in humans. in order to gain insight into the degree of genetic variability of g9p[8] strains circulating in cameroon, stool samples were collected during the 1999-2000 rotavirus season in two different geographic regions in cameroon (southwest and western regions). by rt-pcr, 15 g9p[8] strains (15/89=16.8%) were identified whose gen ... | 2013 | 23770141 |
| simultaneous detection of group a rotavirus in swine and rat on a pig farm in brazil. | this study investigated the occurrence of rotavirus in porcine and rattus norvegicus, at the same time, on a pig farm in the city of jaguariúna, são paulo, brazil. swine (n = 21) and rat (n = 6) fecal samples were analyzed by nested rt-pcr assay. rotavirus occurred in seven porcine and two rat samples. a total of three pig and one rat samples were further submitted to genetic sequencing. the partial nsp5 gene phylogeny showed that all strains were segregated in the genotype h1. these results poi ... | 2013 | 23766702 |
| one year survey of human rotavirus strains suggests the emergence of genotype g12 in cameroon. | in this study the emergence of rotavirus a genotype g12 in children <5 years of age is reported from cameroon during 2010/2011. a total of 135 human stool samples were p and g genotyped by reverse transcriptase pcr. six different rotavirus vp7 genotypes were detected, including g1, g2, g3, g8, g9, and g12 in combinations with p[4], p[6] and p[8] vp4 genotypes. genotype g12 predominated in combination with p[8] (54.1%) and p[6] (10.4%) genotypes followed by g1p[6] (8.2%), g3p[6] (6.7%), g2p[4] (5 ... | 2013 | 23765785 |
| phylogenetic analyses of the vp4 and vp7 genes of porcine group a rotaviruses in sao paulo state, brazil: first identification of g5p[23] in piglets. | this study determined the group a rotavirus occurrence in pig farms from 7 different cities in são paulo state, brazil. out of 143 samples, 70 tested positive. sequence analyses of 37 strains indicated that the strains had the g3, g5, g9, and p[6], p[13]/p[22]-like, and p[23] genotypes. | 2013 | 23761160 |
| molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients. | to describe the genotypic variability of group a rotavirus (rva) found in immunosuppressed and non-immunosuppressed pediatric patients treated at the hospital de clínicas da universidade federal do paraná (hc-ufpr), curitiba, paraná. | 2013 | 23684457 |
| whole-genomic analysis of g3p[23], g9p[23] and g3p[13] rotavirus strains isolated from piglets with diarrhea in thailand, 2006-2008. | group a rotavirus (rva) is the most common cause of severe acute viral gastroenteritis in humans and animals worldwide. this study characterized the whole genome sequences of porcine rvas, 2 g3p[23] strains (cmp40/08 and cmp48/08), 1 g9p[23] strain (cmp45/08), and 1 g3p[13] strain (cmp29/08). these strains were collected from diarrheic piglets less than 7weeks of age in 4 pig farms in chiang mai, thailand, in 2008. the vp7-vp4-vp6-vp1-vp2-vp3-nsp1-nsp2-nsp3-nsp4-nsp5 genes of cmp40/08 and cmp48/ ... | 2013 | 23681022 |
| recombinant monovalent llama-derived antibody fragments (vhh) to rotavirus vp6 protect neonatal gnotobiotic piglets against human rotavirus-induced diarrhea. | group a rotavirus (rva) is the leading cause of severe diarrhea in children. the aims of the present study were to determine the neutralizing activity of vp6-specific llama-derived single domain nanoantibodies (vhh nanoabs) against different rva strains in vitro and to evaluate the ability of g6p[1] vp6-specific llama-derived single domain nanoantibodies (vhh) to protect against human rotavirus in gnotobiotic (gn) piglets experimentally inoculated with virulent wa g1p[8] rotavirus. supplementati ... | 2013 | 23658521 |
| review of group a rotavirus strains reported in swine and cattle. | group a rotavirus (rva) infections cause severe economic losses in intensively reared livestock animals, particularly in herds of swine and cattle. rva strains are antigenically heterogeneous, and are classified in multiple g and p types defined by the two outer capsid proteins, vp7 and vp4, respectively. this study summarizes published literature on the genetic and antigenic diversity of porcine and bovine rva strains published over the last 3 decades. the single most prevalent genotype combina ... | 2013 | 23642647 |
| feline origin of rotavirus strain, tunisia, 2008. | in tunisia in 2008, an unusual g6p[9] rotavirus, rva/human-wt/tun/17237/2008/g6p[9], rarely found in humans, was detected in a child. to determine the origin of this strain, we conducted phylogenetic analyses and found a unique genotype constellation resembling rotaviruses belonging to the feline ba222-like genotype constellation. the strain probably resulted from direct cat-to-human transmission. | 2013 | 23631866 |
| impact of rotavirus vaccine on rotavirus genotypes and caliciviruses circulating in french cattle. | group a rotaviruses are a leading cause of neonatal calf diarrhoea worldwide and prevention of this disease includes vaccination against these viruses. in order to highlight the potential selection of rotavirus genotypes due to immune pressure driven by vaccination, the aim of this study was to compare group a rotavirus genotypes circulating in french diarrhoeic calves in rotavirus vaccinated herds (g6p[5] vaccine) with those in non-vaccinated herds during one calving season in 2010. this study ... | 2013 | 23579256 |
| phylogenetic analysis of g1p[6] group a rotavirus strains detected in northeast brazilian children fully vaccinated with rotarix™. | in 2009 the world health organization recommended the use of group a rotavirus (rva) vaccines in all national immunization programs (nips) in order to control severe rva gastroenteritis disease. in brazil, rotarix™ was introduced in the nip in march 2006, and a significant reduction in mortality rates among children ≤ 5 years old was observed, especially in the northern and northeastern brazil. in the current study the 11 gene segments of six brazilian g1p[6] rva strains, isolated in 2009 and 20 ... | 2013 | 23538335 |
| continued circulation of g12p[6] rotaviruses over 28 months in nepal: successive replacement of predominant strains. | rotavirus a causes severe diarrhoea in infants and young children worldwide. the migration pattern (electropherotype) of the double-stranded rna genome upon polyacrylamide gel electrophoresis has been used to define "strains" in molecular epidemiology. in temperate countries, distinct electropherotypes (strains) appear after the annual, off-seasonal interruption of rotavirus circulation. in nepal, rotavirus circulated year-round and an uncommon genotype g12p[6] predominated and persisted, provid ... | 2013 | 23533063 |
| evidence for genetic reassortment between human rotaviruses by full genome sequencing of g3p[4] and g2p[4] strains co-circulating in india. | rotavirus a causes severe diarrhoea in infants and young children worldwide. many unusual combinations of g and p genotypes have been observed in rotaviruses circulating in developing countries. mixed infection of a single individual with more than one strain is a mechanism by which genetic reassortants are formed with unusual g and p combinations. however, few studies have provided direct evidence for the formation of such unusual strains as a result of co-infection of co-circulating strains. h ... | 2013 | 23532829 |
| detection and characterisation of rotaviruses from children less than 5 years hospitalised with acute gastroenteritis in nagercoil. | group a rotavirus continues to be the major cause of severe gastroenteritis in young children in developing countries. in this study, we report the prevalence and genotype of rotaviruses identified from children <5 years of age hospitalised with acute gastroenteritis from nagercoil, tamil nadu from 2007-2010. from the 139 children included in the study, 71 samples (51%) were positive by elisa and 65 samples were positive by pcr-based methods. g1p[8] (44.6%) was the most commonly identified genot ... | 2016 | 23508433 |
| identification of cellular calcium binding protein calmodulin as a regulator of rotavirus a infection during comparative proteomic study. | rotavirus (rv) being the major diarrhoegenic virus causes around 527000 children death (<5 years age) worldwide. in cellular environment, viruses constantly adapt and modulate to survive and replicate while the host cell also responds to combat the situation and this results in the differential regulation of cellular proteins. to identify the virus induced differential expression of proteins, 2d-dige (two-dimensional difference gel electrophoresis) based proteomics was used. for this, ht-29 cell ... | 2013 | 23437200 |
| comparison of different rt-qpcr assays for the detection of human and bovine group a rotaviruses and characterization by sequences analysis of genes encoding vp4 and vp7 capsid proteins. | the aim of this study was to compare the performance of four rt-qpcr assays for the detection of human and bovine group a rotaviruses and to characterize the positive samples by sequence analysis of vp4 and vp7 genes. | 2013 | 23421708 |
| complex evolutionary patterns of two rare human g3p[9] rotavirus strains possessing a feline/canine-like h6 genotype on an au-1-like genotype constellation. | the group a rotavirus (rva) g3p[9] is a rare vp7-vp4 genotype combination, detected occasionally in humans and cats. other than the prototype g3p[9] strain, rva/human- tc/jpn/au-l/1982/g3p3[9], the whole genomes of only two human g3p[9] rva strains and two feline g3p[9] rva strains have been analyzed so far, revealing complex evolutionary patterns, distinct from that of au-1. we report here the whole genomic analyses of two human g3p[9] rva strains, rva/human-tc/chn/l621/2006/g3p[9] and rva/huma ... | 2013 | 23403096 |
| sequence analysis of the whole genomes of five african human g9 rotavirus strains. | the g9 rotaviruses are amongst the most common global rotavirus strains causing severe childhood diarrhoea. however, the whole genomes of only a few g9 rotaviruses have been fully sequenced and characterised of which only one g9p[6] and one g9p[8] are from africa. we determined the consensus sequence of the whole genomes of five african human group a g9 rotavirus strains, four g9p[8] strains and one g9p[6] strain collected in cameroon (central africa), kenya (eastern africa), south africa and zi ... | 2013 | 23369762 |
| detection and genetic diversity of porcine group a rotaviruses in historic (2004) and recent (2011 and 2012) swine fecal samples in ohio: predominance of the g9p[13] genotype in nursing piglets. | epidemiological surveillance of porcine group a rotavirus (rva) strains was conducted in five swine herds in ohio using historical (2004) and recent (2011 to 2012) fecal samples. of the 371 samples examined, 9.4% (35/371) were positive for rva. the rva detection rates increased from 5.9% in 2004 and 8.5% in 2011 to 13.8% in 2012. a total of 23 positive samples were analyzed for rva g and p genotypes. the dominant g-p combination was g9p[13] found in 60.9% of positive samples. the other combinati ... | 2013 | 23363823 |
| changes in anti-group a rotavirus antibody seroprevalence and levels in the western gyeongnam province of korea over 16 years. | to observe how anti-group a rotavirus antibody seropositivity rates and levels have changed in the western region of gyeongnam province, 2,030 serum samples collected at four collection periods (1989-1990, 1994-1995, 1999-2000, and 2004-2005) were tested by enzyme-linked immunosorbent assay for igg, and iga antibodies reacting to recombinant vp6 protein. the seroprevalences exhibit no regular patterns over a 16-yr period. for all four collection periods, the anti-rvp6 igg levels rose steadily du ... | 2013 | 23341712 |
| characterization of human rotaviruses circulating in iraq in 2008: atypical g8 and high prevalence of p[6] strains. | fecal samples from 976 children with gastroenteritis were collected and analyzed for group a rotavirus (rva), in three different cities in iraq between january 2008 and december 2008. rva antigen was detected in 394 (40%) of the samples, and 98 samples were available for further genotype analyses using multiplex rt-pcr and sequence analyses for untypeable strains. the g/p-genotype combination was determined for 69 samples, and 19, 2 and 8 samples remained p-untypeable, g-untypeable and g/p-untyp ... | 2013 | 23340225 |
| evolutionary time-scale of primate bocaviruses. | human bocavirus (hbov) is associated with acute gastroenteritis in humans, occurring mostly in young children and elderly people. four bocavirus genotypes (hbov1-hbov4) have been found so far. since there were no data on the contribution of hbov to gastroenteritis in russia, 1781 fecal samples collected from infants hospitalized with acute gastroenteritis in novosibirsk, russia during one year were tested for the presence of nucleic acids from hbov and three major gastrointestinal viruses (rotav ... | 2013 | 23313830 |
| rotavirus infection among sudanese children younger than 5 years of age: a cross sectional hospital-based study. | in sudan, rotavirus has been one of the important causative agents of diarrhea among children. rotavirus a is well known as the leading cause of diarrhea in young children worldwide. it was estimated to account for 41% of hospitalized cases of acute gastroenteritis among children in sub-saharan africa. this study aimed to determine the prevalence and the common clinical presentations of rotavirus a infection among sudanese children with gastroenteritis seeking management in hospitals. | 2013 | 24711878 |
| group a rotavirus universal mass vaccination: how and to what extent will selective pressure influence prevalence of rotavirus genotypes? | two human group a rotavirus (rva) vaccines are available and highly effective in preventing severe gastroenteritis caused by all commonly circulating human rva genotypes. the effect of universal mass vaccination on the rva genotype distribution is discussed based on the knowledge of complete rva genotype constellations, data from clinical efficacy trials and effectiveness studies, and genotype surveillance data from countries with universal mass vaccination programs. the theoretically predicted ... | 2012 | 23249234 |
| [genetic diversity of group a rotavirus isolates found in western siberia in 2007-2011]. | genetic analysis of group a rotavirus recovered from fecal samples of children admitted to hospitals in novosibirsk and omsk during four epidemic seasons 2007, 2007/2008, 2009/2010, 2010/2011 was performed. a total of 1416 rotavirus isolates were genotyped using multiplex pcr. the isolates of the most common rotavirus genotypes g1p[8], g4p[8], g2p[4], g3p[8] co-circulated in western siberia during 2007-2011. in isolated cases g9p[8], g2p[8], g3p[9], and g4p[6] genotypes were detected. change of ... | 2012 | 23248851 |
| analysis of rotavirus species diversity and evolution including the newly determined full-length genome sequences of rotavirus f and g. | rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. eight different rotavirus species (a-h) have been defined based on antigenicity and nucleotide sequence identities of the vp6 gene. here, the first complete genome sequences of rotavirus f (strain 03v0568) and g (strain 03v0567) with lengths of 18,341 and 18,186bp, respectively, are described. both viruses have open reading frames for rotavirus proteins vp1 to vp7 and nsp1 to nsp5 located at the 11 genome segme ... | 2013 | 23237956 |
| the origin of two rare human p[10] rotavirus strains. | the group a rotavirus (rva) p[10] is a rare genotype of the rva vp4 gene. to date, the whole genome sequence of only a single p[10] rva strain, rva/human-tc/idn/69m/1980/g8p4[10], has been determined, revealing a ds-1-like genotype constellation. whole genomic analyses of p[10] rva strains with other vp7 genotypes are essential to obtain conclusive data on the origin and genetic diversity of the p10] rvas. in the present study, the whole genome of a human g4p[10] rva strain, rva/human-tc/idn/57m ... | 2013 | 23178318 |
| multiplex real-time pcr assays for the detection of group c rotavirus, astrovirus, and subgenus f adenovirus in stool specimens. | group c rotavirus (gcrv), astrovirus (astv), and adenovirus (subgenus f adenov) are etiologic agents of acute nonbacterial gastroenteritis, which often represents community outbreaks. for the efficient detection of gcrv, astv, and subgenus f adenov in stool specimens, a multiplex real-time pcr assay was developed to detect these three viruses simultaneously, with high sensitivity and specificity. in total, 8404 clinical specimens were collected between april 2008 and march 2011 and tested for gc ... | 2013 | 23159674 |
| antibody response in vaccinated pregnant mares to recent g3bp[12] and g14p[12] equine rotaviruses. | both the g3p[12] and the g14p[12] type of equine group a rotavirus (rva) have recently become predominant in many countries, including japan. g3 types are classified further into g3a and g3b. the g3a viruses have been circulating in europe, australia, and argentina, and the g3b viruses have been circulating in japan. however, only an inactivated vaccine containing a single g3bp[12] strain is commercially available in japan. to assess the efficacy of the current vaccine against recently circulati ... | 2012 | 23130609 |
| burden and typing of rotavirus group a in children with acute gastroenteritis in shiraz, southern iran. | human rotavirus is a significant cause of severe gastroenteritis in infants and young children worldwide. in recent years, rotavirus genotyping by rt-pcr has provided valuable information about the diversity of rotaviruses circulating worldwide. the purpose of the present study is to monitor the prevalence of the different g types of rotaviruses circulating in shiraz, southern iran and detect any uncommon or novel types. | 2012 | 23115715 |
| molecular characterization analysis of the outer protein layer (vp7) from human rotavirus a genotype g1 isolate identified in iran: implications for vaccine development. | the full open reading frame of the outer protein layer vp7 from an isolate of human rotavirus identified in 2010 in an iranian child admitted to hospital with gastroenteritis was amplified from a clinical stool specimen and subjected to molecular characterization. genetic and phylogenetic analyses indicated that the analyzed gene falls into the g1 genotype forming a sub-cluster with sequences recently identified in iran and geographically distant countries. such results were confirmed by protein ... | 2012 | 23109009 |
| molecular characterization of group a rotavirus found in elderly patients in ireland; predominance of g1p[8], continued presence of g9p[8], and emergence of g2p[4]. | rotavirus is a major cause of gastroenteritis in young children worldwide. there have been several recent reports concerning rotavirus isolation from adults, particularly in the elderly, presenting with gastroenteritis. in this study, the authors report on rotavirus outbreaks in five separate elderly care facilities between april, and june 2011 in ireland. the following genotypes were detected; g1p[8] (n = 5/11), g2p[4] (n = 2/11), and g9p[8] (n = 2/11). thus, similarities to previous reports we ... | 2012 | 23080510 |
| prevalence of group a genotype human rotavirus among children with dirarrhea in thailand, 2009-2011. | rotavirus is the most common cause of severe diarrhea in infants and young children world-wide, with the highest fatality rate in developing countries. we investigated the presence and seasonal distribution of group a rotavirus infection among thai children. the data will be used for vaccine development. samples were collected from infants and children with acute gastroenteritis or diarrhea admitted to two hospitals between june 2009 and may 2011. group a rotaviruses were detected in 250 (44.5%) ... | 2012 | 23077813 |
| identification of an avian group a rotavirus containing a novel vp4 gene with a close relationship to those of mammalian rotaviruses. | group a rotaviruses (rvas) are an important cause of diarrhoeal illness in humans, as well as in mammalian and avian animal species. previous sequence analyses indicated that avian rvas are related only distantly to mammalian rvas. here, the complete genomes of rva strain 03v0002e10 from turkey (meleagris gallopavo) and rva strain 10v0112h5 from pheasant (phasianus colchicus) were analysed using a combination of 454 deep sequencing and sanger sequencing technologies. an adenine-rich insertion si ... | 2013 | 23052396 |
| the validation and utility of a quantitative one-step multiplex rt real-time pcr targeting rotavirus a and norovirus. | rotavirus (rov) and norovirus (nov) are the main causes of viral gastroenteritis. currently, there is no validated multiplex real-time pcr that can detect and quantify rov and nov simultaneously. the aim of the study was to develop, validate, and internally control a multiplex one-step rt real-time pcr to detect and quantify rov and nov in stool samples. pcr sensitivity was assessed by comparing amplification against the current gold standard, enzyme immunoassay (eia), on stool samples from 94 i ... | 2013 | 23046990 |
| rotavirus genotypes in malaysia and universal rotavirus vaccination. | group a rotavirus (rv-a) genotypes isolated in malaysia was studied to estimate the effectiveness of a universal rv-a vaccination in malaysia. a simple mathematical model was used, with input from a two-year, two-center, prospective study on hospitalization of rv-a gastroenteritis (rvge) in young children, published data on rv-a hospitalizations and genotypes, mortality on childhood ge and published genotype-specific efficacy data on two rv-a vaccines. assuming a 95% vaccine coverage, the overal ... | 2012 | 23022710 |
| frequency of group a rotavirus with mixed g and p genotypes in bovines: predominance of g3 genotype and its emergence in combination with g8/g10 types. | the present study describes the genotypic distribution of rotaviruses (rvs) in an indian bovine population with unexpectedly higher proportions of g3 alone or in combination of g8/g10. pcr-genotyping confirmed that 39.4% (13/33) of the prevalent rvs were the g3 type while 60.6% (20/33) were dual g3g10 or g3g8 types. p typing revealed that 93.9% (31/33) of the samples were p[11] while 6.1% (2/33) possessed a dual p[1]p[11] type. sequence analysis of the vp7 gene from g3 strains viz. b-46, 0970, a ... | 2012 | 23006956 |
| the first identification of rare human group a rotavirus strain g3p[10] with severe infantile diarrhea in eastern india. | during an ongoing surveillance for diarrheal pathogens, an unusual human group a rotavirus strain g3p[10] (rva/human-wt/ind/mcs60/2011/g3p[10]) was detected in a stool sample of a 14 months old girl child with acute diarrhea in kolkata, eastern india. the vp7 nucleotide sequence of this strain revealed a close phylogenetic relationship to the prototype g3 strain au-1 and australian feline strain cat2, whereas, the vp4 gene segment was closely related to the g8p[10] rotavirus 69m from indonesia. ... | 2012 | 22981998 |
| rotavirus in diarrheal children in rural burkina faso: high prevalence of genotype g6p[6]. | group a rotavirus (rva) is the most common cause of severe gastroenteritis in young children globally, and responsible for a significant number of deaths in african countries. while vaccines are available, trials have shown a lesser efficacy in africa. one of the reasons could be the prevalence and/or emergence of unusual or novel rva strains, as many strains detected in african countries remain uncharacterized. in this study, we characterized rva positive specimens from two remote rural areas i ... | 2012 | 22964045 |
| equine g3p[3] rotavirus strain e3198 related to simian rrv and feline/canine-like rotaviruses based on complete genome analyses. | equine group a rotavirus (rva) strains are the most important cause of gastroenteritis in equine neonates and foals worldwide, and g3p[12] and g14p[12] are epidemiologically the most important genotypes. the genotype constellation of an unusual argentinean g3p[3] rva strain (rva/horse-wt/e3198/2008/g3p[3]) detected in fecal samples of a diarrheic foal in 2008 was shown to be g3-p[3]-i3-r3-c3-m3-a9-n3-t3-e3-h6. each of these genotypes has been found typically in feline and canine rva strains, and ... | 2013 | 22959604 |
| rotavirus genotyping in gastroenteritis cases of an infantile population from western brazilian amazonia. | during the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the western brazilian amazon. | 2012 | 22930050 |
| discovery and molecular characterization of a group a rotavirus strain detected in an argentinean vicuña (vicugna vicugna). | the wild vicuña (vicugna vicugna) is one of the four species of native south american camelids (sacs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. serological data have indicated the presence of group a rotaviruses (rva) specific antibodies in all 4 members of the sac, and so far, rva has been detected from alpacas, llamas and guanacos. a total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in jujuy ... | 2013 | 22877519 |
| detection of avian group d rotavirus using the polymerase chain reaction for the vp6 gene. | group d rotaviruses (rvs-d) have been documented in birds and, while they may be common in these animals, few molecular studies are available for this specific group. in this study, specific primers for the gene that encodes for the rvs-d vp6 protein were designed and used in a reverse transcription polymerase chain reaction (rt-pcr). thirty pools of samples were tested by polyacrylamide gel electrophoresis (page) yielding a 30% (9/30) positivity. these pools were subjected subsequently to rt-pc ... | 2012 | 22820073 |
| full genome analysis of group b rotaviruses from western india: genetic relatedness and evolution. | to date, full-genome sequences of only seven human group b rotavirus (rvbs) strains have been described. such data on more rvbs are necessary to establish the evolutionary relationship and ecological features of rvbs from different geographical regions. the present study was aimed at determining the full-length sequences of all 11 genes of 13 human rvb strains detected during 1995-2010 in sporadic and outbreak cases of acute gastroenteritis from four different cities of western india. this study ... | 2012 | 22815276 |
| prevalence and factors associated with group a rotavirus infection among children with acute diarrhea in mwanza, tanzania. | rotavirus infections frequently cause acute gastroenteritis in humans and are the most important cause of severe dehydrating diarrhea in young children in both developed and developing countries. | 2012 | 22706194 |
| genotype constellation and evolution of group a rotaviruses infecting humans. | numerous rotavirus group a (rva) strains with distinct g-genotype and p-genotype combinations have been described infecting humans worldwide. however, the increasing amount of complete rva genome data which have become available, suggest that only rva strains with 2 discrete genotype constellations have been successful in sustaining infection of humans worldwide over longer periods of time. those genotype constellations have been designated i1-r1-c1-m1-a1-n1-t1-e1-h1 and i2-r2-c2-m2-a2-n2-t2-e2- ... | 2012 | 22683209 |
| epidemiology of rotavirus diarrhea in children under 5 years in northern cameroon. | rotavirus still remains the major cause of diarrhea in children below 5 years. no data on rotavirus epidemiology is available in the northern regions of cameroon. we aimed to determine the prevalence of group a rotavirus (rva) in children below 5 years with diarrhea in two regions of northern cameroon (north west and far north regions) so as to improve our knowledge on the burden of rotavirus disease for imminent introduction of a rotavirus vaccine. | 2012 | 22655107 |
| real-time pcr detection of enteric viruses in source water and treated drinking water in wuhan, china. | a total of 48 water samples were collected from six water treatment plants in wuhan and analyzed by real-time pcr assay for viral identification of enterovirus (ev), rotavirus group a (rva), human adenovirus (hadv) as well as human adenovirus subgroup f (hadvf) during the period from december 2010 to october 2011. hadv, hadvf, and rva were all positively detected in the samples of source water and treated drinking water. ev could be found in 46 % (11/24) of all the source water samples, but only ... | 2012 | 22645016 |
| selection and evolutionary analysis in the nonstructural protein nsp2 of rotavirus a. | rotavirus a is the leading cause of acute gastroenteritis in infants and young children worldwide. the nonstructural protein 2 (nsp2) plays essential roles in the replication cycle of rotavirus and may play a role in protective immunity against rotavirus disease. using a bayesian approach, we measured the mutation rate of genotype n1 nsp2 gene sequences. the n1 genotype is the main nsp2 genotype associated with rotavirus strains causing severe disease, and was found to have a high mutation rate ... | 2012 | 22610044 |
| whole-genomic analysis of a human g1p[9] rotavirus strain reveals intergenogroup-reassortment events. | group a rotavirus (rva) strain k8 (rva/human-tc/jpn/k8/1977/g1p[9]) was found to have wa-like vp7 and nsp1 genes and au-1-like vp4 and nsp5 genes. to determine the exact origin and overall genetic makeup of this unusual rva strain, the remaining genes (vp1-vp3, vp6 and nsp2-nsp4) of k8 were analysed in this study. strain k8 exhibited a g1-p[9]-i1-r3-c3-m3-a1-n1-t3-e3-h3 genotype constellation, not reported previously. the vp6 and nsp2 genes of strain k8 were related closely to those of common hu ... | 2012 | 22592265 |
| the unpredictable diversity of co-circulating rotavirus types in europe and the possible impact of universal mass vaccination programmes on rotavirus genotype incidence. | this article reviews the incidence of group a rotavirus (rv) types isolated from children with acute gastroenteritis (age) in european countries during the last 5-10 years, with the aim of establishing an overview of rv diversity before the introduction of universal mass vaccination (umv) programmes against rv disease in most european countries. consistent with findings from previous surveys, a high degree of diversity of co-circulating rv types exists in different locations of europe, and over ... | 2012 | 22579864 |
| comparison of two eukaryotic systems for the expression of vp6 protein of rotavirus specie a: transient gene expression in hek293-t cells and insect cell-baculovirus system. | the vp6 protein of rotavirus a (rva) is a target antigen used for diagnostic assays and also for the development of new rva vaccines. we have compared the expression of vp6 protein in human embryonic kidney (hek293-t) cells with results obtained using a well-established insect cell-baculovirus system. the recombinant vp6 (rvp6) expressed in hek293-t cells did not present degradation and also retained the ability to form trimers. in the insect cell-baculovirus system, rvp6 was expressed at higher ... | 2012 | 22576283 |
| repeated circulation over 6 years of intergenogroup mono-reassortant g2p[4] rotavirus strains with genotype n1 of the nsp2 gene. | human rotavirus a, a major cause of acute diarrhoea in infants and young children worldwide, contains the genome of 11 segments of double-stranded rna, which is grouped into three major genotype constellations: the wa genogroup (g1-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1), the ds-1 genogroup (g2-p[4]-i2-r2-c2-m2-a2-n2-t2-e2-h2), and the au-1 genogroup (g3-p[9]-i3-r3-c3-m3-a3-n3-t3-e3-h3). recently, a g2p[4] strain detected in kenya in 1982 was reported to be a nonstructural protein 2 (nsp2) gene mono-re ... | 2012 | 22575821 |
| genotypic linkages of gene segments of rotaviruses circulating in pediatric patients with acute gastroenteritis in thailand. | rotavirus is a major cause of morbidity and mortality of infants and young children with diarrhea throughout the world. in thailand, extensive studies of rotavirus infections have been reported continually and rotavirus diarrhea remains a common illness. to monitor the epidemiological situation of rotavirus in chiang mai, thailand, surveillance of rotavirus circulating in pediatric patients was conducted. a total of 160 fecal specimens collected from children hospitalized with diarrhea were test ... | 2012 | 22564696 |
| analysis of the excretion dynamics and genotypic characteristics of rotavirus a during the lives of pigs raised on farms for meat production. | to determine the excretion dynamics and genotypic characteristics of rotavirus a (rva), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. a total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day intervals. seventy-one samples (17.5%) were positive for rva by reverse transcription-pcr designed to detect the vp7 and vp4 genes. at least 13 combinations of 5 g (g2, g4, g5, g9, an ... | 2012 | 22493330 |
| assessment of gastroenteric viruses frequency in a children's day care center in rio de janeiro, brazil: a fifteen year study (1994-2008). | this 15-year study aimed to determine the role of the main viruses responsible for acute infantile gastroenteritis cases in a day care center in the city of rio de janeiro, brazil. from 1994 to 2008, 539 fecal samples were obtained from 23 outbreaks as well as sporadic cases that occurred in this period. the detection of rotavirus group a (rva), norovirus (nov) and astrovirus (astv) was investigated both by classical and molecular methods of viral detection. rva was detected by enzymatic immune ... | 2012 | 22448271 |
| comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in australia. | compare real-time reverse transcription polymerase chain reaction (qrt-pcr), a commercially available enzyme-linked immunosorbent assay (elisa) and lateral flow immunochromatography assay (lat) for the detection of rotavirus and coronavirus in faecal samples collected from diarrhoeic calves. | 2012 | 22443326 |
| evidence for the porcine origin of equine rotavirus strain h-1. | equine group a rotavirus (rva) strain h-1 (rva/horse-tc/gbr/h-1/1975/g5p9[7]) was found to have vp4, vp6-7, nsp1 and nsp4 genes of porcine origin. in order to obtain conclusive information on the exact origin and evolution of this unusual equine strain, the remaining six genes (vp1-3, nsp2-3 and nsp5 genes) of strain h-1 were analyzed in the present study. by whole genomic analysis, strain h-1 exhibited a porcine rva-like genotype constellation (g5-p[7]-i5-r1-c1-m1-a8-n1-t1-e1-h1), different fro ... | 2012 | 22437009 |
| full genome characterization of a porcine-like human g9p[6] rotavirus strain isolated from an infant in belgium. | interspecies transmissions of group a rotavirus (rva) strains among animals and humans are thought to take place frequently. during a rva surveillance study in belgium we isolated an unusual g9p[6] rva strain, rva/human-wt/bel/be2001/2009/g9p[6], from a 1month old boy, which did not cluster with other g9 or p[6] strains isolated in belgium. in this study we sequenced and characterized the complete genome of this unusual g9p[6] strain be2001. phylogenetic analyses of all 11 genes revealed a uniqu ... | 2012 | 22430049 |
| genetic variation in the vp7 gene of rotavirus g1p[8] strains isolated in vietnam, 1998-2009. | group a rotavirus genotype g1p[8] is the most common strain affecting humans around the world over the past few decades. in this study, we examined genetic variation in the vp7 gene of rotavirus g1p[8] strains, detected in children of four major cities of vietnam during three different rotavirus seasons: 1998-1999, 2007-2008 and 2008-2009 in order to assess the evolution of the virus over 11 years. fecal samples (n=73) from children hospitalized for gastroenteritis caused by g1p[8] rotavirus wer ... | 2012 | 22387966 |
| group a rotavirus genotypes circulating prior to implementation of a national immunization program in estonia. | group a rotaviruses (rva) are a major cause of acute gastroenteritis in children ≤ 5 y worldwide which could be prevented with two recently introduced vaccines - monovalent rotarix (live-attenuated g1p[8] strain) and pentavalent rotateq (human-bovine reassortant containing serotypes g1, g2, g3, g4 and p[8]). prior to implementation of vaccines into national immunization program we aimed to describe rva genotype distribution in hospitalized children aged < 5 y in estonia during 2007-2008. a total ... | 2012 | 22370511 |
| a large outbreak of enteritis in goat flocks in marmara, turkey, by g8p[1] group a rotaviruses. | group a rotaviruses are regarded as major enteric pathogens of large ruminants, while their impact on the health of small ruminants is not well documented. we report the detection of group a rotavirus from a large outbreak of enteritis that occurred in two goat flocks in the town of kırklareli, marmara region, turkey, in 2007. the disease was observed in young kids, with high morbidity and mortality rates, but not in adult animals. rotavirus antigen was detected in the stools of the examined ani ... | 2012 | 22367501 |
| phylogenetic analysis of the rotavirus genotypes originated from children < 5 years of age in 16 cities in south korea, between 2000 and 2004. | the purpose of this study was to examine the diversity of the g and p types of human rotavirus strains isolated in south korea during 2000 to 2004. | 2012 | 24159485 |
| predominance of rotavirus genotype g6p[11] in diarrhoeic lambs. | out of 500 faecal samples from lambs with diarrhoea in jammu and kashmir, india, 66 (13.2%) were positive for group a rotavirus (garv) by the latex agglutination test (lat). electropherotyping by rna-polyacrylamide gel electrophoresis revealed the typical garv 4-2-3-2 migration pattern in 49/66 (74.2%) samples. fifty-two samples (10.4%) were positive by reverse transcription-pcr. g6 was the predominant g genotype (25/52; 48.07%), followed by g10 (19/52; 36.54%) whereas, the predominant p genotyp ... | 2012 | 22230710 |
| genomic diversity among group a rotaviruses from diarrheic children, piglets, buffalo and cow calves of madhya pradesh. | diarrheal disease continues to be a global health problem, particularly among young ones in developing nations. amongst several viral and non-viral agents associated with diarrhea, group a rotavirus has been recognized as the major etiological agent of childhood gastroenteritis in human infants as well as several animal species throughout the world. during this study, a total of 181 diarrheic stool samples collected from children, piglets, buffalo and cow calves of madhya pradesh, central india ... | 2010 | 23100812 |
| detection of human rotavirus in hospitalized diarrheic children in central india. | during the present study, group a human rotaviruses were detected among diarrheic children using polyacrylamide gel electrophoresis (page) technique, with a typical rna migration pattern of 4:2:3:2, suggestive of group a rotavirus. during the study, a total of 46 fecal samples collected from hospitalized children with acute diarrhea as well as children inhabiting nearby animal farms with history of presence of animal rotaviruses on the farms were processed for detection of human rotavirus. out o ... | 2007 | 23100693 |
| igy antibodies protect against human rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model. | group a rotaviruses are the most common cause of severe, dehydrating diarrhea in children worldwide. the aim of the present work was to evaluate protection against rotavirus (rv) diarrhea conferred by the prophylactic administration of specific igy antibodies (ab) to gnotobiotic piglets experimentally inoculated with virulent wa g1p[8] human rotavirus (hrv). chicken egg yolk igy ab generated from wa hrv hyperimmunized hens specifically recognized (elisa) and neutralized wa hrv in vitro. suppleme ... | 2012 | 22880110 |
| rotavirus inhibits ifn-induced stat nuclear translocation by a mechanism that acts after stat binding to importin-α. | the importance of innate immunity to rotaviruses is exemplified by the range of strategies evolved by rotaviruses to interfere with the ifn response. we showed previously that rotaviruses block gene expression induced by type i and ii ifns, through a mechanism allowing activation of signal transducer and activator of transcription (stat) 1 and stat2 but preventing their nuclear accumulation. this normally occurs through activated stat1/2 dimerization, enabling an interaction with importin α5 tha ... | 2014 | 24814927 |
| molecular detection of viruses in kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses. | this is the first country-wide surveillance of bat-borne viruses in kenya spanning from 2012-2015 covering sites perceived to have medium to high level bat-human interaction. the objective of this surveillance study was to apply a non-invasive approach using fresh feces to detect viruses circulating within the diverse species of kenyan bats. we screened for both dna and rna viruses; specifically, astroviruses (astvs), adenoviruses (advs), caliciviruses (calvs), coronaviruses (covs), flaviviruses ... | 2017 | 28393313 |
| phylogenetic analysis of vp1 and rdrp genes of brazilian aichivirus b strains involved in a diarrhea outbreak in dairy calves. | aichivirus b has been reported worldwide in calves and adult cattle with and without diarrhea. the aim of this study was to describe the molecular characteristics of the rdrp and vp1 genes of aichivirus b strains identified as the most frequent etiologic agent in a neonatal diarrhea outbreak in a high-production brazilian dairy cattle herd. preliminary laboratory analysis ruled out important enteropathogens (cryptosporidium spp; eimeria spp., e. coli f5, and bovine coronavirus). fecal samples fr ... | 2017 | 28849283 |
| expression of bovine rotavirus vp8 and preparation of igy antibodies against recombinant vp8. | group a rotavirus is the leading cause of acute gastroenteritis in cattle and swine. although, vaccination against this virus is an effective strategy for prevention, additional strategy to control disease is necessary. egg yolk immunoglobulin (igy)-based passive immunization could be a better option in preventing this disease. bovine rotavirus (brv) is group a rotavirus and possesses a genome of 11 segments of double-stranded rna. the outer layer of capsid is composed of two proteins (vp7 and v ... | 2017 | 28523920 |
| development and validation of monoclonal antibody-based antigen capture elisa for detection of group a porcine rotavirus. | porcine rotavirus-a (porva) is one of the common causes of mild to severe dehydrating diarrhea, leading to losses in weaning and postweaning piglets. a rapid, highly specific, and sensitive antigen-capture enzyme-linked immunosorbent assay (ac-elisa) was developed for detection of porva, by using vp6 (a highly conserved and antigenic protein of group-a rotavirus)-directed rabbit polyclonal antibodies (capture antibody) and murine monoclonal antibodies (detector antibody). the detection limit of ... | 2017 | 28414586 |
| genotypic and epitope characteristics of group a porcine rotavirus strains circulating in canada. | surveillance of rotavirus a (rva) infections in north america swine populations are limited and not performed over a significant time period to properly assess the diversity of rva strains in swine. the vp7 (g) and vp4 (p) genes of 32 canadian rva strains, circulating between 2009 and 2015 were sequenced, identifying the g3p[13], g5p[7], g9p[7], g9[13], and g9[19] genotype combinations. the canadian rva strains were compared to the rva strains present in the swine prosystems rce rotavirus vaccin ... | 2017 | 28399437 |
| development of a multiplex real-time pcr assay for detection of human enteric viruses other than norovirus using samples collected from gastroenteritis patients in fukui prefecture, japan. | there are many varieties of gastroenteritis viruses, of which norovirus (nov) accounts for over 90% of the viral food poisoning incidents in japan. however, protocols for rapidly identifying other gastroenteritis viruses need to be established to investigate nov-negative cases intensively. in this study, a multiplex real-time pcr assay targeting rotavirus a, rotavirus c, sapovirus, astrovirus, adenovirus, and enterovirus was developed using stool samples collected from gastroenteritis patients b ... | 2017 | 28845896 |
| complex reassortment events of unusual g9p[4] rotavirus strains in india between 2011 and 2013. | rotavirus a (rva) is the predominant etiological agent of acute gastroenteritis in young children worldwide. recently, unusual g9p[4] rotavirus strains emerged with high prevalence in many countries. such intergenogroup reassortant strains highlight the ongoing spread of unusual rotavirus strains throughout asia. this study was undertaken to determine the whole genome of eleven unusual g9p[4] strains detected in india during 2011-2013, and to compare them with other human and animal global rvas ... | 2017 | 28750901 |
| reassortant ds-1-like g1p[4] rotavirus a strains generated from co-circulating strains in vietnam, 2012/2013. | one major mechanism by which rotavirus a (rva) evolves is genetic reassortment between strains with different genotype constellations. however, the parental strains of the reassortants generated have seldom been identified. here, the whole genome of two suspected reassortants, rva/human-wt/vnm/sp127/2013/g1p[4] and rva/human-wt/vnm/sp193/2013/g1p[4], with short rna electropherotypes were examined by illumina miseq sequencing and their ancestral phylogenies reconstructed. their genotype constella ... | 2017 | 28696017 |
| whole genome characterisation of a porcine-like human reassortant g26p[19] rotavirus a strain detected in a child hospitalised for diarrhoea in nepal, 2007. | a rare g26 rotavirus a strain rva/human-wt/npl/07n1760/2007/g26p[19] was detected in a child hospitalised for acute diarrhoea in kathmandu, nepal. the complete genome of 07n1760 was determined in order to explore its evolutionary history as well as examine its relationship to a vietnamese strain rva/human-wt/vnm/30378/2009/g26p[19], the only g26 strain whose complete genotype constellation is known. the genotype constellation of 07n1760 was g26-p[19]-i12-r1-c1-m1-a8-n1-t1-e1-h1, a unique constel ... | 2017 | 28673546 |
| completely genomic and evolutionary characteristics of human-dominant g9p[8] group a rotavirus strains in yunnan, china. | g9p[8] rotavirus a (rva) has been identified as the predominant genotype circulating in yunnan, china. to elucidate the molecular characteristics of its genetic composition at the whole-genome level, the genomes of 12 strains isolated from paediatric patients with diarrhoea were fully sequenced and characterized. eleven of the 12 strains were genotyped as g9-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1, which is closely related to the wa-like genotype 1 constellation. in contrast, one strain was genotyped as ... | 2017 | 28613141 |
| phylogenetic analysis of g1p[8] and g12p[8] rotavirus a samples obtained in the pre- and post-vaccine periods, and molecular modeling of vp4 and vp7 proteins. | reduction in morbimortality rates for acute gastroenteritis (age) by rotavirus a (rva) has been observed after the introduction of vaccines, however the agent continues to circulate. the present study described the genomic characterization of the 11 dsrna segments of two rva samples g1p[8] obtained in the pre- and post-vaccination periods and one of g12p[8] sample (post-vaccine), compared to rotarix™ vaccine. analysis by molecular sequencing of the samples showed that the three samples belonged ... | 2017 | 28606817 |
| detection of uncommon g3p[3] rotavirus a (rva) strain in rat possessing a human rva-like vp6 and a novel nsp2 genotype. | rotavirus is one of the leading causes of acute gastroenteritis in infants and young children. rvas infect not only humans but also a wide range of mammals including rats, which represent a reservoir of several other zoonotic pathogens. due to the segmented nature of the rva genome, animal rva strains can easily adapt to the human host by reassortment with co-infecting human viruses. this study aims to detect and characterize rva in the intestinal content of italian sinantropic rats (rattus ratt ... | 2017 | 28603021 |
| epidemiology of rotavirus infection in children from a rural and urban area, in maputo, southern mozambique, before vaccine introduction. | this study aimed to describe the epidemiology of rotavirus infections in mozambique before vaccine introduction. between february 2012 and september 2013, stool specimens, demographic and clinical data were collected from 384 children <5 years old hospitalized with acute diarrhea in mavalane general hospital and manhiça district hospital, southern mozambique. the samples were tested for rotavirus a using enzyme-linked immunosorbent assay. the overall prevalence of rotavirus infection was 42.4% [ ... | 2017 | 28582541 |
| a universal genome sequencing method for rotavirus a from human fecal samples which identifies segment reassortment and multi-genotype mixed infection. | genomic characterization of rotavirus (rov) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material. | 2017 | 28438140 |
| group a rotavirus associated with encephalitis in red fox. | in 2011, a group a rotavirus was isolated from the brain of a fox with encephalitis and neurologic signs, detected by rabies surveillance in italy. intracerebral inoculation of fox brain homogenates into mice was fatal. genome sequencing revealed a heterologous rotavirus of avian origin, which could provide a model for investigating rotavirus neurovirulence. | 2017 | 28820385 |
| viral metagenomics of six bat species in close contact with humans in southern china. | accumulating studies have shown that bats could harbor various important pathogenic viruses that could be transmitted to humans and other animals. extensive metagenomic studies of different organs/tissues from bats have revealed a large number of novel or divergent viruses. to elucidate viral diversity and epidemiological and phylogenetic characteristics, six pooled fecal samples from bats were generated (based on bat species and geographic regions characteristic for virome analysis). these cont ... | 2018 | 28983731 |
| identification of two novel rotavirus a genotypes, g35 and p[50], from peruvian alpaca faeces. | rotavirus a (rva) alp11b was detected from a neonatal peruvian alpaca presenting with diarrhea, and the alp11b vp7, vp4, vp6, nsp4, and nsp5 genes were sequenced. the partial genotype constellation of this strain, rva/alpaca-wt/per/alp11b/2010, was determined to be g35-p[50]-i13-e16-h6. | 2017 | 28866138 |
| prevalence of lapine rotavirus, astrovirus, and hepatitis e virus in canadian domestic rabbit populations. | lapine rotavirus and astrovirus have been associated with disease in rabbits, and there is strong evidence of zoonotic transmission of lapine hepatitis e virus (hev). outbreaks of enteritis are common on commercial meat farms, resulting in poor welfare, high rabbit mortality, and significant financial losses for rabbit producers. currently, none of these viruses are routinely tested by diagnostic laboratories. in this study, we assessed the prevalence of rotavirus, astrovirus, and hev rna in 205 ... | 2017 | 28888629 |
| identification of group a rotaviruses from zambian fruit bats provides evidence for long-distance dispersal events in africa. | group a rotavirus (rva) is a major cause of diarrhea in children worldwide. although rva infects many animals, little is known about rva in bats. the present study investigated the genetic diversity of rva in zambian bats. we identified rva from two straw-colored fruit bats (eidolon helvum) and an egyptian fruit bat (rousettus aegyptiacus), and analyzed the genome sequences of these strains. genome segments of the rva strains from zambian e. helvum showed 97%-99% nucleotide sequence identity wit ... | 2018 | 29792990 |
| epidemiology and molecular characterization of rotavirus a in fruit bats in bangladesh. | rotavirus a (rva) is the primary cause of acute dehydrating diarrhea in human and numerous animal species. animal-to-human interspecies transmission is one of the evolutionary mechanisms driving rotavirus strain diversity in humans. we screened fresh feces from 416 bats (201 pteropus medius, 165 rousettus leschenaultii and 50 taphozous melanopogon) for rva using rrt-pcr. we detected a prevalence of 7% (95% ci 3.5-10.8) and 2% (95% ci 0.4-5.2) in p. medius and r. leschenaultii, respectively. we d ... | 2020 | 32876756 |
| cynomolgus monkeys (macaca fascicularis) as an experimental infection model for human group a rotavirus. | group a rotaviruses (rva) are one of the most common causes of severe acute gastroenteritis in infants worldwide. rotaviruses spread from person to person, mainly by faecal⁻oral transmission. almost all unvaccinated children may become infected with rva in the first two years of life. the establishment of an experimental monkey model with rva is important to evaluate new therapeutic approaches. in this study, we demonstrated viral shedding and viraemia in juvenile⁻adult macaca fascicularis orall ... | 2018 | 29973483 |
| molecular characterization of group a rotavirus from rhesus macaques (macaca mulatta) at human-wildlife interfaces in bangladesh. | group a rotavirus (rva) is an important cause of diarrhoea in people, especially children, and animals globally. due to the segmented nature of the rva genome, animal rva strains have the potential to adapt to the human host through reassortment with other co-infecting human viruses. macaques share food and habitat with people, resulting in close interaction between these two species. this study aimed to detect and characterize rva in rhesus macaques (macaca mulatta) in bangladesh. faecal sample ... | 2020 | 31765042 |
| whole genome sequence analysis of cell culture-adapted rotavirus a strains from chicken. | rotavirus a (rva) is a major cause of gastroenteritis in humans and mammalian animals, and has also been abundantly detected in avian species. avian rva infection is associated with diarrhea, reduced growth and increased mortality, leading to economic losses in the poultry industry. avian rva forms a unique genetic clade within the whole rva species. however, up to now, only a few full-length avian rva genomes have been published and only a small number of avian rva strains have been adapted to ... | 2020 | 32147474 |
| genetic diversity and zoonotic potential of rotavirus a strains in the southern andean highlands, peru. | interspecies transmission is an important mechanism of evolution and contributes to rotavirus a (rva) diversity. in order to evaluate the detection frequency, genetic diversity, epidemiological characteristics and zoonotic potential of rva strains in faecal specimens from humans and animals cohabiting in the same environment in the department of cusco, peru, by molecular analysis, 265 faecal specimens were obtained from alpacas, llamas, sheep and shepherd children, and tested for rva by rt-pcr. ... | 2019 | 31002476 |
| leptospira spp., rotavirus, norovirus, and hepatitis e virus surveillance in a wild invasive golden-headed lion tamarin (leontopithecus chrysomelas; kuhl, 1820) population from an urban park in niterói, rio de janeiro, brazil. | the world currently faces severe biodiversity losses caused by anthropogenic activities such as deforestation, pollution, the introduction of exotic species, habitat fragmentation, and climate changes. disease ecology in altered environments is still poorly understood. the golden-headed lion tamarin (ghlt, leontopithecus chrysomelas) is an endangered species that became invasive in an urban park in niterói, rio de janeiro, brazil. the initially few invasive ghlt individuals became hundreds, adap ... | 2019 | 30828830 |
| identification of a novel clade of group a rotaviruses in fatally diseased domestic pigeons in europe. | rotaviruses are well-known causative agents of enteric disorders in humans and other mammals, but little is known about their virulence and pathogenic role in pigeons and other birds. starting in summer 2017, a series of outbreaks of an acute disease with high mortalities was reported in domestic pigeons in germany, belgium and denmark. the clinical picture was characterized by diarrhoea, vomiting, hepatic necrosis and sudden fatalities. from these severe outbreaks, we discovered several previou ... | 2019 | 30407742 |
| genotype constellation of a rotavirus a field strain with an uncommon g8p[11] genotype combination in a rotavirus-vaccinated dairy cattle herd. | in this report we describe the genotype constellation of a bovine rotavirus a (rva) strain with an uncommon g8p[11] genotype combination. the rva/cow-wt/bra/y136/2017/g8p[11] strain was classified as g8-p[11]-i2-r5-c2-m2-a3-n2-t9-e2-h3. phylogenetic analysis based on the vp7 gene showed that the y136 strain and a human g8p[1] strain comprise a putative new (vii) lineage for the g8 genotype. in addition, two other genotypes, r5 (vp1) and t9 (nsp3), were identified in the constellation of y136 tha ... | 2020 | 32472289 |
| g and p genotype profiles of rotavirus a field strains circulating in beef and dairy cattle herds in brazil, 2006-2015. | the aim of this retrospective study was to use rt-pcr and nucleotide sequencing analysis to determine the g (vp7 gene) and p (vp4 gene) genotypes of 155 brazilian bovine rotavirus a (rva) wild-type strains detected in diarrheic calves from all brazilian geographical regions from 2006 to 2015. the rva strains evaluated belonged to the g6, g10, p[5], and p[11] genotypes. the g6p[5] genotype was prevalent (65.5%; p < 0.05) in beef, and the g10p[11] (38.4%) and g6p[11] (30.8%) genotypes were more pr ... | 2019 | 31174706 |
| longitudinal surveillance of rotavirus a genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine. | worldwide, neonatal diarrhea is one of the most important health issues affecting dairy calves, and rotavirus a (rva) is one of its primary causes. among the measures to mitigate the risk of diarrhea outbreaks, cow vaccination stands out as one of the most important. however, the immune pressure resulting from routine vaccination may be able to select specific g and p genotypes in rva field strains. this study aimed to determine the frequency and intensity of neonatal diarrhea and the incidence ... | 2019 | 30827398 |