| predictive value of respiratory syncytial virus-specific ige responses for recurrent wheezing following bronchiolitis. | to determine whether the magnitude of the respiratory syncytial virus (rsv)-specific ige response at the time of an episode of rsv bronchiolitis in infancy accurately predicts the development of subsequent wheezing episodes, we observed 38 infants prospectively from the time of an episode of infantile bronchiolitis through 48 months of age. peak rsv-ige titers were measured at the time of the bronchiolitis episode using an elisa procedure. notation was made of both the number of subsequent wheez ... | 1986 | 3772657 |
| human respiratory syncytial virus glycoprotein g expressed from a recombinant vaccinia virus vector protects mice against live-virus challenge. | recombinant vaccinia virus vectors were constructed which expressed the major surface glycoprotein g of human respiratory syncytial (rs) virus. the biological activity of the g protein expressed from these vectors was assayed. inoculation of rabbits with live recombinant virus induced high titers of antibody which specifically immunoprecipitated rs virus g protein and was capable of neutralizing rs virus infectivity. immunization of mice by either the intranasal or the intraperitoneal route with ... | 1986 | 3773052 |
| gianotti-crosti syndrome associated with infections other than hepatitis b. | although the gianotti-crosti syndrome (gcs) is regularly associated with hepatitis b infection elsewhere, in north america that association is rarely made. accordingly, we studied nine children with acral, symmetrical eruptions typical of gcs for evidence of other infections. all were negative for hepatitis b surface antigen. viral cultures were done in nine patients, and viruses isolated in two. one patient with a respiratory prodrome had respiratory syncytial virus (rsv) isolated, and a second ... | 1986 | 3773143 |
| the use of eye-nose goggles to control nosocomial respiratory syncytial virus infection. | we evaluated an eye-nose goggle to determine its usefulness in reducing nosocomial rsv infection in patients and staff members on our infant ward. during a community outbreak of rsv in 1984, infection was assessed by biweekly routine viral cultures on all ward personnel and patients and also by seroconversion in personnel. for three weeks staff members wore the goggles; two (5%) adults and one (6%) child acquired nosocomial infection. during the subsequent three-week study period, goggles were n ... | 1986 | 3773177 |
| respiratory syncytial virus surveillance in various regions across canada. | | 1987 | 3791104 |
| infections due to respiratory syncytial virus in canada. | | 1987 | 3791105 |
| age related igg subclass response to respiratory syncytial virus fusion protein in infected infants. | respiratory syncytial virus (rsv) fusion protein was purified by immunoaffinity chromatography using a mouse monoclonal antibody coupled to affi-gel 10. the fusion protein was homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and free of other detectable viral or cellular protein. the purified fusion protein was used in a quantitative enzyme-linked immunosorbent assay (elisa) to measure the age-related antibody response to this protein in infected infants. the f ... | 1986 | 3791688 |
| effect of breast feeding on responses of systemic interferon and virus-specific lymphocyte transformation in infants with respiratory syncytial virus infection. | in order to elucidate the mechanisms of breast-feeding-induced resistance to respiratory syncytial virus (rsv) infection, groups of breast-fed and bottle-fed infants with this infection were tested at the onset of illness and then again 1 and 2 weeks later for the presence of interferon (ifn) as well as of virus-specific lymphocyte transformation (ltf) activity. alpha-ifn was detected in nine out of ten breast-fed infants, while it was found in only 11 out of 21 bottle-fed subjects. the rate of ... | 1987 | 3794673 |
| virus-specific ige and igg4 antibodies in serum of children infected with respiratory syncytial virus. | | 1987 | 3794891 |
| the pediatrician's role in influenza control. | influenza is the most important of acute respiratory illnesses that cause persons to seek medical care. for infants some other viruses such as respiratory syncytial and parainfluenza viruses share in the assault but influenza is the only respiratory virus for which we have tools for prevention. we need to use these tools effectively to build a wall of protection around vulnerable patients. the fact that influenza vaccine does not have the same efficacy as other pediatric vaccines should not disc ... | 1986 | 3797294 |
| respiratory syncytial virus and acute lower respiratory infections in benin city, nigeria. | | 1986 | 3797309 |
| expression of the fusion protein of human respiratory syncytial virus from recombinant vaccinia virus vectors and protection of vaccinated mice. | vaccinia virus (vv) recombinants were constructed that contained full-length cdna copies of the fusion (f) protein gene of human respiratory syncytial (rs) virus. the f protein gene was placed next to the strong early-late vv 7.5-kilodalton promoter and was located within the vv thymidine kinase (tk) gene. full-length recombinant transcripts that initiated at both the tk and the 7.5-kilodalton promoters accumulated in cells early in infection, and one or more of these transcripts was translated ... | 1987 | 3806789 |
| possible association of mycoplasma and viral respiratory infections with bacterial meningitis. | the presence of viral infection was evaluated in 160 children older than three months with bacterial meningitis who were admitted to children's medical center or parkland memorial hospital, dallas, tx, between october 1979 and march 1982. results were compared with a single serologic specimen in 138 children without meningitis. a recent history of upper respiratory infection was obtained from 60% of patients, including 10/13 with pneumococcal, 9/16 with meningococcal, and 77/131 with haemophilus ... | 1987 | 3812456 |
| american academy of pediatrics committee on infectious diseases: ribavirin therapy of respiratory syncytial virus. | | 1987 | 3822659 |
| serologic survey of viral antibodies in the peruvian alpaca (lama pacos). | sera from more than 100 alpacas (lama pacos) from the peruvian southern sierra were examined for antibodies to 8 viruses known to infect other domestic animals. on the basis of these serologic findings and previously published serologic or clinical data, it is now known that the alpaca can be infected with the following viruses: parainfluenza-3, bovine respiratory syncytial virus, bovine herpesvirus-1, bluetongue virus, border disease virus, influenza a virus, rotavirus, rabies virus, vesicular ... | 1987 | 3826854 |
| fatal pneumonia in an adult due to respiratory syncytial virus. | respiratory syncytial virus (rsv) infections in adults are generally mild, and no fatalities in uninstitutionalized adults have been reported. to our knowledge, we document herein the first case of fatal rsv pneumonia in a previously healthy elderly woman living at home, in whom complement fixation titers against rsv rose from less than 1:8 to reach 1:256 at death. cytoplasmic inclusions characteristic of rsv were detected at autopsy. | 1987 | 3827469 |
| purification and characterization of the respiratory syncytial virus fusion protein. | the fusion protein of respiratory syncytial virus was purified by affinity chromatography using a monoclonal antibody. under various conditions the protein was recovered as a 145k dimer or a 70k monomer. the 70k monomer was composed of disulphide-linked fragments of 48k and 23k. polyclonal rabbit serum produced to the dimerized fusion protein neutralized virus but did not inhibit fusion, while rabbit serum to the 2-mercaptoethanol-treated dimerized protein neutralized virus and inhibited fusion ... | 1985 | 3838336 |
| the envelope-associated 22k protein of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polytranscript. | we recently determined that respiratory syncytial virus (strain a2) encodes a fourth unique envelope-associated virion protein that has molecular weight of approximately 24,000, as estimated by gel electrophoresis. the nucleotide sequence of the mrna encoding this novel protein has now been determined from five cdna clones, including three that contain the complete mrna sequence. the complete mrna sequence is 957 nucleotides, exclusive of polyadenylate, and contains two partially overlapping ope ... | 1985 | 3838351 |
| giant cell pneumonia due to respiratory syncytial virus. | | 1985 | 3838449 |
| nucleotide sequence of the respiratory syncytial virus phosphoprotein gene. | a cdna library was prepared from poly(a)+ rna extracted from respiratory syncytial (rs) virus-infected hep-2 cells. a recombinant plasmid, prsp68, encoding the rs virus phosphoprotein gene was identified by translation in vitro of hybrid-selected mrna. the cloned cdna insert of 1 kb was sequenced and a polypeptide of 241 amino acids with a molecular weight of 27 166 was deduced from the sequence. the protein was relatively rich in polar amino acids and devoid of both cysteine and tryptophan. a s ... | 1985 | 3839520 |
| correct sequence for the major nucleocapsid protein mrna of respiratory syncytial virus. | a nucleotide sequence for the mrna of the major nucleocapsid (n) protein gene of respiratory syncytial virus was reported previously (n. elango and s. venkatesen, 1983, nucleic acids res. 11, 5941-5951). however, we have been unable to confirm part of this sequence as n mrna-specific and suggest that the published sequence represents that of an aberrant chimeric transcript. here we present an alternative sequence for the n mrna and provide data supporting its authenticity. the corrected n mrna s ... | 1985 | 3839952 |
| nucleotide sequence of the g protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. | the major surface glycoprotein (g) of human respiratory syncytial (rs) virus has an estimated mature mr of 84,000-90,000. among a library of cdna clones prepared from rs virus mrnas, we identified clones that hybridized to a message that encoded a mr 36,000 polypeptide that was specifically immunoprecipitated with anti-g antiserum. the amino acid sequence of the g protein backbone was determined by nucleotide sequence analysis of several of the cdna clones. it contains a combination of structura ... | 1985 | 3858865 |
| respiratory syncytial virus and heart block--cause and effect? | a child aged 3 years who developed a respiratory syncytial virus infection is described. his admission was complicated by the development of an irregular pulse. he initially had a variable first or second degree heart block with transient electrocardiographic evidence suggestive of pericarditis and/or myocarditis and later developed complete heart block. he has remained asymptomatic for the past four years. a search for other causes of the heart block proved negative. it is tempting to suggest a ... | 1985 | 3859266 |
| fatal respiratory syncytial virus infection in severe combined immunodeficiency syndrome. | we encountered two cases of fatal respiratory syncytial virus infection in infants. both patients had severe anatomic abnormalities in the thymus, probably due to severe combined immunodeficiency syndrome. | 1985 | 3877455 |
| seroprevalence of respiratory syncytial virus in free-ranging bighorn sheep. | sera from 187 (42%) of 447 free-ranging bighorn sheep (ovis canadensis) in 9 western states had antibody titers to respiratory syncytial virus (rsv). titers were from 1:5 to 1:640, as determined by the standard virus neutralization test. results of the survey indicated that rsv is widespread in free-ranging bighorn sheep populations and that rsv may be an important factor in the bighorn sheep pneumonia-complex. | 1985 | 3878355 |
| survey of desert bighorn sheep in california for exposure to selected infectious diseases. | from february 1983 to june 1985, 188 desert bighorn sheep (ovis canadensis nelsoni, = 161 and oc cremnobates, = 27) from 18 herds in 17 mountain ranges and one captive herd were caught, marked, and had blood, fecal, and nasal mucus samples collected. nasal swab specimens were cultured bacteriologically and virologically specifically for parainfluenza-3 (pi-3) virus. bacterial flora differed from herd to herd. pathogenic pneumophilic bacteria (eg, pasteurella sp) seldom were found. parainfluenza- ... | 1985 | 3878356 |
| the 1a protein gene of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polycistronic transcript. | the 1a mrna is the smallest mrna of human respiratory syncytial (rs) virus and encodes a single protein of approximate molecular weight 9500 as estimated previously by gel electrophoresis. the nucleotide sequence of the 1a mrna, determined from several full-length cdna clones, is reported. the 1a mrna consists of 405 nucleotides, exclusive of poly(a), with relatively long nontranslated regions at the 5' and 3' ends (84 and 126 nucleotides, respectively). the sequences at the 5' and 3' termini of ... | 1985 | 3879976 |
| monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions. | we developed five monoclonal antibodies against respiratory syncytial virus. three of these (23a3, 12a4, and 18b2) were used in an indirect fluorescent antibody test, and the results were compared with those of a similar indirect fluorescent test with commercial anti-respiratory syncytial virus serum. the results obtained with antibody 18b2 and commercial anti-respiratory syncytial virus serum were identical, whereas with antibodies 23a3 and 12a4 the incidence of positive identifications was aro ... | 1985 | 3882752 |
| the development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence. | twelve clones of murine hybridoma cells secreting antibody specific for respiratory syncytial (rs) virus were classified into four groups on the basis of their pattern of staining of unfixed rs virus-infected hep-2 cells in an indirect immunofluorescence test. three of the groups reacted with virus antigens present on the membrane of the cells, whilst the fourth group failed to stain most live cells, suggesting specificity for an antigen expressed internally. representative monoclonals from the ... | 1985 | 3884736 |
| respiratory syncytial virus. brief review. | | 1985 | 3885919 |
| effect of in vitro inoculation of bovine respiratory syncytial virus on bovine pulmonary alveolar macrophage function. | viruses may predispose the respiratory tract to the development of secondary bacterial pneumonia by impairing functions of alveolar macrophages. the effects of bovine respiratory syncytial virus (brsv) on selected functions of bovine pulmonary alveolar macrophages (pam) were examined in vitro. alveolar macrophages were obtained from nonsedated cattle, using a polypropylene tube passed intranasally into the lung. the pam lavaged from the lung were allowed to adhere to glass coverslips or plastic ... | 1985 | 3890630 |
| management of bronchiolitis. | the incidence, etiology, epidemiology, clinical presentation, treatment, prognosis, and prevention of bronchiolitis are discussed with a critical evaluation of the available studies on various therapeutic approaches. bronchiolitis is a lower respiratory-tract viral infection that affects 6-10% of all children below two years of age. respiratory syncytial virus (rsv) is the usual pathogen. the symptoms range from mild wheezing to severe respiratory distress. an infected child usually has a fever, ... | 1985 | 3891201 |
| properties of a respiratory syncytial virus isolated from a sheep with rhinitis. | a virus isolated from a yearling cross-bred ewe was identified as respiratory syncytial virus (rsv) by indirect immunofluorescence and by virus neutralization with bovine rsv antisera. the virus caused a mild conjunctivitis in 3-month-old lambs when inoculated alone. although clinical signs of pneumonia were not observed, there was gross and microscopic evidence of pulmonary inflammation in the lungs of lambs inoculated with either the sheep rsv isolate alone or in conjunction with pasteurella h ... | 1985 | 3893243 |
| quantification of respiratory syncytial virus polypeptides in nasal secretions by monoclonal antibodies. | an indirect enzyme-linked immunosorbent assay (elisa) which uses monoclonal antibody as solid-phase immunosorbent was developed to measure specific polypeptides of respiratory syncytial virus (rsv). the assay was used to examine 43 nasopharyngeal (np) aspirates from rsv-positive infants that had been examined previously for rsv by culture, direct immunofluorescence, and polyclonal antibody elisa. frozen np aspirates were serially diluted and examined for the 66k mol. wt. fusion glycoprotein (f), ... | 1985 | 3894575 |
| practical recommendations for the detection of pediatric respiratory syncytial virus infections. | in our private clinic-hospital setting, respiratory syncytial virus (rsv) was isolated from infants more frequently and sooner from nasal washes (84%; 4.2 days) than from throat swabs (45%; 5.5 days) or nasopharyngeal swabs (39%; 5.7 days). immunofluorescence of nasal wash cells identified 72% of the infants with virus isolations from nasal washes in less than one day. we therefore recommend the combination of isolation and immunofluorescence on nasal wash specimens for optimal detection of rsv- ... | 1985 | 3897268 |
| development of cell-mediated cytotoxic activity in the respiratory tract after experimental infection with respiratory syncytial virus. | the development of natural killer cell and other antibody-independent cellular cytotoxic response to rsv were studied in splenic and pulmonary mononuclear effector cells obtained from groups of 6-week-old cotton rats after subcutaneous (sc) or intranasal (in) immunization with live virulent or ultra-violet (uv) inactivated respiratory syncytial virus (rsv). no virus-induced cytotoxic activity was observed after sc immunization with live virus or in inoculation of inactivated non-infectious virus ... | 1985 | 3899431 |
| the production of monoclonal antibodies against respiratory syncytial virus and its clinical applications. | the availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (rsv). the components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic rsv vaccine. a number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative det ... | 1985 | 3899483 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. | a new enzyme-linked immunosorbent assay (elisa) respiratory syncytial virus antigen detection kit (ortho diagnostics, inc., raritan, n.j.) was compared with virus culture and with the indirect fluorescent antibody test (fat) by using fresh nasal washings from children with suspected respiratory syncytial virus infection. both uncentrifuged nasal washings and pellets from centrifuged split specimens were examined by elisa. the elisa was considered positive when the optical density was greater tha ... | 1985 | 3902879 |
| ribavirin treatment of respiratory syncytial viral infection in infants with underlying cardiopulmonary disease. | aerosolized ribavirin was evaluated in the treatment of respiratory syncytial virus lower respiratory tract disease in 53 infants, 36 of whom had underlying diseases. of the total infants, 26 were studied in a double-blind, placebo-controlled manner; 14 received ribavirin and 12 received placebo, a water aerosol, for an average of five days. when the infants with bronchopulmonary dysplasia and congenital heart disease treated with ribavirin were compared with those receiving placebo, the treated ... | 1985 | 3903239 |
| immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat. | human convalescent antiserum to respiratory syncytial virus (rsv) administered intraperitoneally to cotton rats prior to rsv challenge provided near-complete protection from pulmonary infection. antiserum given subsequent to viral challenge reduced pulmonary viral titers 100-fold or greater within 24 h. sandoglobulin, a preparation of purified human igg with high titer of anti-rsv neutralizing activity, produced the same effects as convalescent antiserum. sandoglobulin was absorbed rapidly and p ... | 1985 | 3907188 |
| calf pneumonia. | infectious calf pneumonia is a high-mortality pneumonia of housed dairy-type calves. viruses are important etiologic agents and infection with bovine respiratory syncytial virus (rsv) and parainfluenza type 3 virus (pi-3 virus) may result in extensive, and sometimes fatal, lung damage. respiratory viral infections are frequently followed by mycoplasmal and secondary bacterial invasion of the lower respiratory tract, which increases the extent and severity of lung damage. bad housing, particularl ... | 1985 | 3907774 |
| bovine respiratory syncytial virus. | the current knowledge is reviewed in regards to the importance of bovine respiratory syncytial virus in the bovine respiratory disease complex. the epidemiology, clinical disease, pathologic findings, pathogenesis, diagnosis, treatment, and prevention of this viral disease are discussed. | 1985 | 3907775 |
| respiratory syncytial virus infection in immunosuppressed animals: implications in human infection. | neonatal cotton rats were treated with cyclophosphamide parenterally for three weeks before intranasal inoculation of live respiratory syncytial virus (rsv). immunosuppressive therapy resulted in severe depletion of lymphocytes from the peripheral circulation, the spleen, and the thymus. in contrast to normal rats, immunosuppressed animals developed severe pulmonary pathology with marked infiltration of foamy macrophages. persistent degeneration and regeneration of bronchial epithelial cells wer ... | 1985 | 3908616 |
| [immunofluorescent demonstration of respiratory syncytial virus infection in calves]. | specific high-titre bovine conjugated antisera were obtained against respiratory syncytial virus. use was made of the reference strain nomi and the local isolate antonovo/448. the conjugates produced were shown to have equal qualities to those of the belgian conjugate used for comparison. the anti-antonovo/448 conjugated serum was used in the direct immunofluorescent method to demonstrate a respiratory syncytial virus antigen in organs of animals with acute respiratory diseases as well as to con ... | 1985 | 3909628 |
| [acetone-celloidin method for the cytological and immunofluorescent study of cell cultures infected with various viruses]. | a method was worked out for the extraction of a cell monolayer from test tubes with the use of 10 per cent acetone solution of celloidin. this made it possible to carry out parallel cytologic and immunofluorescent studies of cultures infected with various viruses. in contrast to the alcohol-ether celloidin used in histology the use of the acetone solution of celloidin, suggested by us, preserved to a larger extent the viruses of different structure and composition, and, therefore, was shown to b ... | 1985 | 3909629 |
| [structural aspect of the respiratory syncytial virus genome]. | | 1985 | 3914773 |
| human mononuclear phagocytic cell interaction with some paramyxoviridae. | in consideration of the peculiar relationship between paramyxoviridae and immunocompetent system, we studied interactions of measles virus, newcastle-disease virus and respiratory syncytial virus with human monocytes and macrophages differentiated "in vitro". mononuclear cells were challenged with three different paramyxovirus at different multiplicities of infection. infected cells were then checked for expression of specific viral antigens by immunofluorescent test (fa). moreover, we have eval ... | 1985 | 3916331 |
| respiratory syncytial virus polypeptides. iv. the oligosaccharides of the glycoproteins. | the cell-associated glycoproteins of respiratory syncytial (rs) virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). although present in infected cells, there was no vp70 in purified virus. trypsin treatment of infected cells removed 80 to 90% of vp70 as well as its products vgp48 and gp26. this suggested that most of the vp70 in the cell is located on the plasma membrane. the glycoproteins of purified rs virus (gp1, vgp48 and gp26) contain mannose, galactose and fucose as well as g ... | 1985 | 3919149 |
| correlation of a disease scoring system with arterial po2 values in respiratory syncytial virus infection in calves. | the mean arterial po2 value measured in blood obtained by puncture of the brachial artery of 20 calves with acute clinical signs of a bovine respiratory syncytial virus (brsv) infection was 8.4 +/- 1.9 kpa. the values differed significantly from arterial po2 values of eleven healthy calves (mean 14.2 +/- 1.5 kpa). a disease scoring system is presented based on the type of respiration and the findings on auscultation. a high correlation (r = -0.87) was found between disease scores and arterial po ... | 1985 | 3925615 |
| respiratory syncytial virus outbreak at an institution for the mentally retarded--washington. | | 1985 | 3932832 |
| radiological findings in children with respiratory syncytial virus infection: relationship to clinical and bacteriological findings. | respiratory syncytial virus (rsv) is a frequent cause of bronchiolitis leading to acute admission to hospital in the winter months. a wide range of findings accompanies this disease and the appearances are seldom completely diagnostic. associated bacterial co-infections are common and we have shown an association with atelectasis among patients with pathogenic bacteria in the nasopharynx. | 1986 | 3951895 |
| vapors, viruses, and views. ribavirin and respiratory syncytial virus. | | 1986 | 3953524 |
| seroepizootiologic study of bovine respiratory syncytial virus in a dairy herd. | a 16-month seroepizootiologic study of bovine respiratory syncytial virus (brsv) infection was conducted in a dairy herd. results indicated that antibodies to brsv present in serum from newborn calves were derived through the ingestion of colostrum. this passive immunity in calves became undetectable in an average of 99 days (sd = 36.5; range = 30 to 208 days). two epizootics of respiratory tract disease occurred during the study period, and an association with brsv was demonstrated in both epiz ... | 1986 | 3954198 |
| seroepizootiologic study of bovine respiratory syncytial virus in a beef herd. | a seroepizootiologic study of bovine respiratory syncytial virus in a beef herd was conducted from february 1983 through march 1984. during the study period, 3 separate respiratory tract disease epizootics were recognized in calves after they had been split into 3 groups. bovine respiratory syncytial virus infection was diagnosed in each epizootic on the basis of serologic evidence, postmortem findings, and immunofluorescent examination of lung tissue. additionally, there was serologic evidence ... | 1986 | 3954199 |
| complement activation by respiratory syncytial virus-infected cells. | respiratory syncytial virus (rsv), a major respiratory pathogen of children, has been speculated to cause disease by immunologic mechanisms. although circulating levels of complement (c) are normal during rsv infections, the role of c in respiratory tract secretion is unclear. since epithelial cells of the respiratory tract of children infected with rsv express viral surface antigens, the ability of rsv infected human cells to activate c was studied. rsv infected human cells (hela) were found to ... | 1986 | 3954599 |
| serological and virological investigations of young children with acute respiratory syndrome associated with respiratory syncytial virus. | from january 1979 to march 1979, 341 young children from the metropolitan area of naples, italy, were hospitalized with respiratory virus disease. diagnosis of patients made from virus isolation and seroconversion indicate that the respiratory syncytial virus was a primary cause of this acute respiratory syndrome. | 1985 | 3967475 |
| antibody response to respiratory syncytial virus structural proteins in children with acute respiratory syncytial virus infection. | the purified respiratory syncytial virus (rsv), randall strain contained 10 polypeptides (72,000 molecular weight [72k], 66k, 48k, 42k, 40k, 36k, 30k, 23k, 18k, and 15k), 8 of which proved to be virus specific, and polypeptides 48k and 23k were glycosylated. in addition, a high-molecular-weight (150k), virus-specific glycopolypeptide was immunoprecipitated from rsv-infected cell lysate. the antibody response in human sera serially collected from children with primary rsv infection was mainly dir ... | 1985 | 3973971 |
| monoclonal capture antibody elisa for respiratory syncytial virus: detection of individual viral antigens and determination of monoclonal antibody specificities. | an enzyme-linked immunosorbent assay (elisa) for respiratory syncytial virus (rsv) that employs solid-phase monoclonal antibodies was developed. rsv antigens bound by these monoclonal capture antibodies were detected by addition of a polyclonal bovine antiserum, followed anti-bovine enzyme conjugate and enzyme substrate. the sensitivity and specific of the assay were determined by titrations of the solid-phase antibodies and by antigen titrations with both unpurified rsv-infected cell culture ma ... | 1985 | 3981003 |
| [clinical aspects of acute and chronic respiratory syncytial virus infection in adult polyclinic patients]. | a total of 224 workers and employees engaged at the smolensk flax spinning plant, suffering from acute respiratory diseases, were examined on an outpatient basis. virus etiology of the diseases was established in 84 patients (37.7%) with the aid of the serological tests (hi and cf) and immunofluorescent techniques. respiratory syncytial virus infection was diagnosed in 19 patients (8.5%). as regards the clinical picture the symptoms of intoxication were unmarked. half the patients demonstrated m ... | 1985 | 3983830 |
| [experimental infection of lambs with strains of the bovine respiratory syncytial virus]. | lambs were successfully infected with bovine strains of a respiratory syncytial virus. when the nomi reference strain and the local antonovo strain of the rsv were used i/v and via the trachea the animals reacted on the 3rd to 5th day with dizziness, dyspnea, conjunctivitis, nasal discharges, etc., more strongly manifested with the use of the antonovo strain. the temperature reaction started on the 3rd day and receded on the 7th day, with a second peak on the 8h day. serologically, rsv-antibodie ... | 1985 | 3992914 |
| characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. | a total of 13 respiratory syncytial (rs) virus specific polypeptides were identified by pulse-chase metabolic labeling of infected hep-2 cells. ten of the 13 proteins were shown to be unique. they were the l, g, f (f1, f2), n, p, m, 24k, 14k, 11k and 9.5k proteins. these conclusions were based on peptide mapping and on previous work showing that each of 10 polypeptides are coded for by a unique mrna. the seven largest proteins, l, g, f (f1, f2), n, p, m and 24k were identified clearly as virion ... | 1985 | 3993233 |
| experimental bovine respiratory syncytial virus infection in conventional calves: light microscopic lesions, microbiology, and studies on lavaged lung cells. | conventionally raised male holstein calves, 1 month of age, were infected by intranasal and intratracheal inoculation with bovine respiratory syncytial virus. viral antigen was identified by fluorescence microscopy most commonly in the cytoplasm of tracheal and bronchial epithelial cells 3 to 5 days after inoculation. cytoplasmic viral antigen was identified also in nasal, nasopharyngeal, bronchiolar, and alveolar epithelial cells and in alveolar macrophages. bronchitis and tracheitis, character ... | 1985 | 3994121 |
| experimental bovine respiratory syncytial virus infection in conventional calves: ultrastructural respiratory lesions. | the morphogenesis and repair of airway and alveolar injury induced by bovine respiratory syncytial virus (brsv) was studied ultrastructurally in conventional calves to characterize pulmonary cell types susceptible to viral infection and cytopathologic changes associated with infection. viral nucleocapsids and budding virions were present in tracheal and bronchial ciliated and nonciliated epithelial cells and mucous cells 3, 5, and 7 days after inoculation and in bronchiolar ciliated and noncilia ... | 1985 | 3994122 |
| lobar collapse with respiratory syncytial virus pneumonitis. | in a study of 30 children with uncomplicated respiratory syncytial virus (rsv) pneumonias, a high incidence of lobar collapse (8/30-26%) was noted. this involved the right upper lobe in seven patients and the left upper lobe in one patient. it is probably attributable to anatomical predispositions, sloughing of necrotic epithelium, and stimulation of mucus production. lobar collapse should be considered part of the spectrum of rsv pneumonitis. | 1985 | 4000751 |
| respiratory syncytial virus-specific ige responses following infection: evidence for a predominantly mucosal response. | in order to determine whether ige production occurs predominantly at mucosal or systemic sites, we studied the production of respiratory syncytial virus (rsv)-specific antibody in serum and nasopharyngeal secretions (nps) from 41 patients with rsv infection using an enzyme-linked immunosorbent assay. rsv-ige was found in higher titer in samples of nps than in simultaneously obtained serum specimens at all phases of illness. despite the excess dilution incurred in the collection process, rsv-ige ... | 1985 | 4000767 |
| severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome. | respiratory syncytial virus (rsv) frequently causes bronchiolitis in young infants. rsv infection was established by culture, serologic response, immunofluorescence of secretions or lung tissue or typical histology on pathologic examination of the lungs in 10 patients who were hospitalized at children's medical center, dallas. eight were infants 4 days to 3 months old, one was 9 months and one was 16 months old. seven patients were afebrile but had rapidly progressive diffuse infiltrates on ches ... | 1985 | 4000987 |
| respiratory syncytial virus polypeptides. v. the kinetics of glycoprotein synthesis. | the cell-associated glycoproteins of respiratory syncytial virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). the time course of virus protein synthesis in hela cells revealed that the nucleocapsid protein (vpn41) was the first to appear at 11 h post-infection followed by the appearance of the other viral proteins at 16 h post-infection. pulse-chase experiments with [3h]leucine or [35s]methionine demonstrated that the precursor for vgp48 and gp26 was an uncleaved protein of 70k (v ... | 1985 | 4009147 |
| mrna sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22k structural protein. | an mrna sequence of two human respiratory syncytial viral nonstructural protein genes and of a gene for a 22,000-molecular-weight (22k) protein was obtained by cdna cloning and dna sequencing. sequences corresponding to the 5' ends of the respective transcripts were deduced directly by primer extension and dideoxy nucleotide sequencing of the mrnas. the availability of a bicistronic clone (prsc6) confirmed the gene order for this portion of the genome. contrary to other unsegmented negative-stra ... | 1985 | 4009789 |
| comparison of a newly developed enzyme-linked immunosorbent assay with complement fixation and neutralisation tests for serology of bovine respiratory syncytial virus infections. | an indirect double antibody sandwich enzyme-linked immunosorbent assay (elisa) was developed for the detection and titration of serum antibodies to bovine respiratory syncytial virus (brsv). the elisa was compared with a complement fixation (cf) test and a test for virus neutralising antibody in serum (virus neutralisation [vn] test). testing sera collected in dairy herds revealed the closest correlation between the results of the elisa and the cf test with respect to brsv antibody titres. the v ... | 1985 | 4012035 |
| an outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward. | a small outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward during the period december 1982 to march 1983 is reported. it demonstrates that respiratory syncytial virus infection can cause serious lower respiratory tract infection even in patients without any previous history of chest disease. | 1985 | 4013906 |
| respiratory tract infections. goals for 1995. | goals to be identified for 1995, a decade hence, in the prevention, diagnosis, and management of respiratory tract infections may conveniently be divided into diagnostic goals and goals in therapy and prophylaxis. major diagnostic goals for bacterial, viral, and mycoplasmal infections of the respiratory tract focus on the development of systems to identify microbial components, such as specific antigens or segments of dna, using monoclonal antibody techniques or dna probes for hybridization. spu ... | 1985 | 4014289 |
| serologic studies of bovine respiratory syncytial virus in minnesota cattle. | serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. serum samples were obtained through the minnesota state-federal brucellosis laboratory and were collected over a 1-year period. results of this study revealed an antibody prevalence of 65.5% to bovine respiratory syncytial virus. the distribution of antibody titers is presented, as well as analysis of titers based on breed, sex, and age of the cattle. | 1985 | 4014838 |
| etiology of community-acquired pneumonia in patients requiring hospitalization. | the etiology of community-acquired pneumonia was studied in 127 patients with roentgenologically verified pneumonia who needed hospitalization. etiology was determined on the basis of a positive blood culture and/or a significant antibody titer increase. streptococcus pneumoniae was the probable etiological agent in 69 patients, nontypeable haemophilus influenzae in five patients, streptococcus pyogenes in two patients, and legionella pneumophila and staphylococcus aureus in one patient each. ev ... | 1985 | 4018066 |
| quantitative aspects of passive immunity to respiratory syncytial virus infection in infant cotton rats. | the amount of passively acquired serum respiratory syncytial virus (rsv)-neutralizing antibodies required to protect the respiratory tract of cotton rats against infection was studied. infant cotton rats were inoculated intraperitoneally with various dilutions of a single pool of sera derived from cotton rats convalescent from rsv infection. after 24 h, these animals were inoculated with rsv intranasally. virus replication in the respiratory tract was suppressed in cotton rats which had a serum ... | 1985 | 4020957 |
| [incidence of respiratory syncytial virus infection in case records of a pediatric general medicine department]. | | 1985 | 4021934 |
| kinetics of synthesis of respiratory syncytial virus glycoproteins. | the synthesis of the two respiratory syncytial (rs) virus glycoproteins (vp66 and vp84) was examined under standard conditions and after treatment with tunicamycin and monensin. the protein backbone for vp66, the fusion protein (f1,2) is cotranslationally glycosylated to form f0, which is cleaved to form f1,2 by 20 min of chase. monensin treatment inhibited the cleavage of f0 over an 80 min chase period, indicating that this occurred late in the transit of f0 through the golgi apparatus or after ... | 1985 | 4031826 |
| the childhood health effects of an improved water supply system on a remote panamanian island. | the incidence of diarrhea, respiratory disease, and skin infections was prospectively determined after the introduction of a system which distributed unlimited quantities of high quality fresh water to each of the 150 housing units on tupile, an island devoid of fresh water located off panama's caribbean coast and inhabited by 1,500 cuna indians. tupile residents used 7.1 liters of water/person/day compared to the 2.3 usage rate of inhabitants on achutupo, the control island. despite ready avail ... | 1985 | 4037183 |
| outbreak of respiratory syncytial virus in france. | a report is given of an outbreak of respiratory syncytial virus infection in a neonatal unit in france. twenty-three of 32 infants were infected (72%) despite infection control procedures. prophylactic administration of non-specific gamma globulins was associated with a significant decrease in infection rate (p less than 0.05). the administration of transfer factor to infected infants was also associated with a significantly lower rate of severe respiratory diseases (p less than 0.05). | 1985 | 4043060 |
| studies of passive immunotherapy for infections of respiratory syncytial virus in the respiratory tract of a primate model. | | 1985 | 4045247 |
| cellular reactivity to respiratory syncytial virus in human colostrum and breast milk. | colostrum and breast-milk samples were taken from 23 mothers between 2 days and 7 weeks postpartum and were examined for the presence of cellular reactivity to respiratory syncytial (rs) virus using a lymphocyte transformation assay. positive responses were detected in nine of the 23 (39%) samples taken at 2-5 days postpartum, but this reactivity was undetectable at 3 weeks. positive responses developed in a further three mothers during the 3-7-week period of lactation, suggesting a response to ... | 1985 | 4045438 |
| severe respiratory disease in dairy cattle in new york state associated with bovine respiratory syncytial virus infection. | severe respiratory disease associated with bovine respiratory syncytial virus (brsv) infection has been identified in dairy cattle in new york state. the cases identified occurred in dairy calves and heifers. the disease was characterized in 4 animals by pathologic changes including interstitial pneumonia, necrotizing bronchiolitis with multinucleated syncytial epithelial cells and interstitial emphysema. brsv antigen was demonstrated in lung samples or was isolated in tissue culture in all 4 ca ... | 1985 | 4053607 |
| an epizootic of respiratory syncytial virus infection in a dairy herd. | respiratory syncytial virus was the cause of a severe epizootic of bovine respiratory tract disease. the virus, isolated from a sick cow during the epizootic, produced cytopathic effect in a bovine turbinate cell line 14 days after it was inoculated. additional support for the diagnosis came from the results of pathologic and serologic examinations. lesions consistently present were severe necrotizing bronchiolitis and epithelial syncytia projecting from bronchiolar and alveolar walls. also, in ... | 1985 | 4055489 |
| severe rsv bronchiolitis in an immunocompromised child. | a 9-year-old with immunodeficiency developed a severe, diffuse respiratory illness that necessitated mechanical ventilation. open lung biopsy revealed respiratory syncytial virus (rsv) as the sole pathogen. rsv detection should be included in the differential diagnosis of diffuse lung disease in an immunocompromised child. | 1985 | 4064461 |
| microneutralization test for respiratory syncytial virus based on an enzyme immunoassay. | virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. after an appropriate incubation period, the cells were fixed, and an enzyme-linked immunosorbent assay was performed directly in the microtiter plates for detection of virus. results could be read and recorded automatically, which is especially helpful when running large numbers of tests. | 1985 | 4066916 |
| comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and hep-2 cell culture. | two hundred seventy nasopharyngeal aspirates were tested in duplicate with the ortho diagnostics, inc. (raritan, n.j.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. the test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. the enzyme-linked immunosorbent assay detected seven antigen-positive specimens not among the 71 specimens that were positive for respiratory syncytial virus in cell culture. a blocking test confirmed those specimens as tr ... | 1985 | 4066920 |
| bronchodilator response during acute viral bronchiolitis in infancy. | bronchodilator responsiveness was assessed by measuring specific respiratory conductance before and after inhalation of aerosolized bronchodilator in 50 infants who had acute bronchiolitis due to respiratory syncytial virus infection. thirty per cent of the infants showed an improvement in specific conductance. responders could not be differentiated from nonresponders by family histories of atopy, eosinophil counts, or immunoglobulin levels in blood and nasal secretions. eighty-three per cent of ... | 1985 | 4069797 |
| respiratory syncytial virus envelope glycoprotein (g) has a novel structure. | amino acid sequence of human respiratory syncytial virus envelope glycoprotein (g) was deduced from the dna sequence of a recombinant plasmid and confirmed by limited amino acid microsequencing of purified 90k g protein. the calculated molecular mass of the protein encoded by the only long open reading frame of 298 amino acids was 32,588 daltons and was somewhat smaller than the 36k polypeptide translated in vitro from mrna selected by this plasmid. inspection of the sequence revealed a single h ... | 1985 | 4069997 |
| [bovine respiratory syncytial virus as a pathogen in the respiratory tract diseases of cattle: a clinical and seroepidemiologic study in switzerland]. | | 1985 | 4071019 |
| [bovine respiratory syncytial virus (brsv) in switzerland: a serologic study]. | | 1985 | 4071020 |
| [infections with respiratory syncytial virus in infants and young children]. | | 1985 | 4080005 |
| [respiratory syncytial virus: rapid detection of antigen using monoclonal antibodies]. | | 1985 | 4080006 |
| respiratory syncytial virus pneumonia in friesian calves: physiological findings. | clinical, serological and physiological values were measured during and after naturally occurring respiratory syncytial virus pneumonia in four friesian calves weighing between 120 and 160 kg. clinical and serological findings were in agreement with the description of spontaneous calf pneumonia, associated with respiratory syncytial virus infection. pulmonary function values, recorded one, eight, 15 and 57 days after the clinical onset of the disease, provided good evidence that the clinical syn ... | 1985 | 4081337 |
| inhibitory effects of several antiviral compounds on the replication of respiratory syncytial virus in vitro. | | 1985 | 4083870 |
| spontaneous bovine respiratory syncytial virus infections in calves: arterial blood gas, ph and bicarbonate values. | arterial blood gas values of 12 calves in the acute stage of a bovine respiratory syncytial virus infection were compared with corresponding values of 11 healthy calves. the patients appeared to be severely hypoxic. mean (+/- sd) arterial oxygen tension (pao2) values of patients and healthy calves were 7.7 +/- 1.9 and 14.2 +/- 1.5 kpa, respectively. hypercapnia was present in only three of the diseased calves. these findings are in close agreement with findings in young children with respiratory ... | 1985 | 4090240 |
| morphogenesis of respiratory syncytial virus in a green monkey kidney cell line (vero). | the structure and morphogenesis of respiratory syncytial (rs) virus particles in a green monkey kidney cell line (vero) were examined. infected cells contained dense intracytoplasmic inclusions composed of filamentous structures. in places where inclusion material was associated with membranes, structural modifications were induced. there was a thickening of the membrane and an addition of projections 12 to 15 nm in length. the same changes were most frequently observed after association of isol ... | 1970 | 4100527 |
| interferon and respiratory syncytial virus. | | 1971 | 4105357 |
| late stage synchronization of respiratory syncytial virus replication. | | 1971 | 4106351 |
| the cytochemistry of inclusion bodies in cell cultures into which respiratory syncytial virus has been inoculated. | | 1972 | 4112282 |
| structure of bovine respiratory syncytial virus. | | 1973 | 4121663 |
| morphogenesis and ultrastructure of respiratory syncytial virus. | respiratory syncytial (rs) virus was grown in vero cells and fixed for electron microscopy at various stages of maturation. both filamentous and round or kidney-shaped particles, either with (complete) or without (incomplete) internal structure, were observed. all four morphological forms were identical with respect to their reactivity with ferritin-labeled antibody to rs virus. freezeetching revealed a structural feature apparently unique for rs virus, namely helical striations around the core ... | 1973 | 4128827 |