| henoch-schönlein syndrome associated with human parvovirus b19 primary infection. | we report a case of henoch-schönlein syndrome associated with human parvovirus b19 primary infection. the patient, a 27-year-old filipino woman, presented with an erythemato-papular-purpuric eruption localized to the lower limbs. general symptoms and signs included fever, hypotension, nausea, vomiting, abdominal pain, inguinal lymphadenopathy and polyarthralgia. laboratory examinations showed leukocytosis, increase in total serum iga, proteinuria and haematuria. circulating iga immune complexes ... | 1999 | 10210793 |
| parvovirus b19 infection in pregnancy. | transplacental transmission of human parvovirus b19 (b19 virus) to the fetus is an important cause of intrauterine death, abortion, stillbirth, and nonimmune hydrops fetalis. adverse outcome of pregnancy can occur after symptomatic and asymptomatic maternal infection. only rare cases of congenital malformations and fetal disease in live-born infants have been associated with intrauterine b19 virus infection. laboratory results obtained from paired maternal and fetal cord blood samples indicate t ... | 1998 | 10213893 |
| [viral vasculitis not related to hbv, hcv and hiv]. | the hepatitis b and c viruses (hbv and hcv) and the human immunodeficiency virus (hiv) are the main causes of viral vasculitis in humans. the vascular lesions are classically ascribed to a variable combination of circulating immune complex deposition, cryoglobulinemia, and endothelial cell infection. other viruses that can cause human vasculitis include the herpes viruses and parvovirus b19. the usual clinical manifestations of the viral infection are sometimes overshadowed by the vasculitis, wh ... | 1999 | 10214617 |
| [treatment of viral vasculitis]. | necrotizing vasculitis is a heterogeneous group of systemic diseases characterized by inflammation of blood vessel walls. there is current agreement that viral infections can play a central role in the pathophysiology of systemic vasculitides responsible for a broad spectrum of clinical patterns. the hepatitis b and c viruses (hbv and hcv), the parvovirus b19, the human immunodeficiency virus, the htlv 1, and the cytomegalovirus are the viruses most often implicated. only the hbv and hcv are ass ... | 1999 | 10214619 |
| efficacy of a high purity, chemically treated and nanofiltered factor ix concentrate for continuous infusion in haemophilia patients undergoing surgery. | we have evaluated the haemostatic efficacy of plasma derived, highly purified, solvent-detergent treated factor ix concentrate (nanotiv), to which a nanofiltration step has recently been added to improve safety with regard to parvovirus b19, hepatitis a and other nonlipid enveloped viruses. thirteen surgical procedures, including eight orthopaedic operations, were carried out using continuous infusion of nanotiv in 10 haemophilia b patients (nine severe and one mild). tranexamic acid was used fo ... | 1999 | 10215956 |
| human parvovirus b19-associated disease in bone marrow transplantation. | human parvovirus b19 can persist in immunocompromised patients and may produce severe clinical illness. in this retrospective study the incidence of b19-associated infections in bone marrow transplant patients was investigated. during 1 year 60 patients received bone marrow grafts (eight autografts and 52 allogeneic transplantations). in case of early onset, atypical or steroid-resistant erythrodermia the patients' blood and/or tissue specimens were screened for b19 infection by polymerase chain ... | 1999 | 10219641 |
| [parvovirus b19 infection-induced anemia and leukocytopenia after myomectomy]. | a 38-year woman was hospitalized because of myoma uteri. she underwent myomectomy on september 30, 1997 with 2,000 ml blood loss. no blood transfusion was required, but she received a plasma protein product. on the 14th postoperative day, a complete blood count revealed anemia (hb 9.3 g/dl) and leukocytopenia (1,600/ul). but it did not reveal anemia before the operation. bone marrow smears showed erythroblastopenia with giant proerythroblasts. anti-parvovirus b19 igm antibody were positive in th ... | 1999 | 10222671 |
| chronic parvovirus b19 meningitis in a child with acute lymphocytic leukemia. | | 1999 | 10223704 |
| parvovirus b19 infection associated with myocarditis following adult cardiac transplantation. | a 56-year-old woman underwent an uneventful cardiac transplantation due to dilated cardiomyopathy. one week later the patient developed clinical and histological signs of myocarditis. we report for the first time a case of myocarditis in an adult heart transplant recipient, possibly induced by parvovirus b19, as evidenced by the finding of specific igm in serum and specific dna in the myocardial cells. furthermore, this is the first time parvovirus b19 dna has been observed in the myocardium of ... | 1998 | 10225391 |
| the efficacy of therapeutic plasmapheresis for the treatment of fatal hemophagocytic syndrome: two case reports. | a potentially fatal hemophagocytic syndrome (hps) has been noted in patients with reactive hps. we describe 2 patients with reactive hps treated with a regimen of therapeutic plasmapheresis and evaluate the efficacy of plasmapheresis for fatal hps. case 1 was a 31 year-old woman who had been treated for systemic lupus erythematosus (sle) with corticosteroid hormones and immunosuppressants. she presented with persistent leukopenia and thrombocytopenia with spiking fever. she had an elevated level ... | 1998 | 10227760 |
| you're the flight surgeon. parvovirus-associated polyarthropathy. | | 1999 | 10233009 |
| severe nonimmune hydrops fetalis and congenital corneal opacification secondary to human parvovirus b19 infection. a case report. | in parvovirus infections in animals, congenital anomalies are seen, but the teratogenic potential in humans seems fairly low. | 1999 | 10319311 |
| [parvovirus-b19 infection in pregnancy: a cause of nonimmune hydrops fetalis]. | | 1999 | 10321268 |
| [parvovirus b19 infection in pregnancy: a cause of non-immune hydrops fetalis]. | | 1999 | 10321269 |
| acute cerebellar ataxia with human parvovirus b19 infection. | a 2 year old boy developed acute cerebellar ataxia in association with erythema infectiosum. during the disease, genomic dna and antibodies against human parvovirus b19 were detected in serum but not in cerebrospinal fluid. parvovirus b19 associated acute cerebellar ataxia might occur due to transient vascular reaction in the cerebellum during infection. | 1999 | 10325764 |
| fetal parvovirus b19 infection associated with myocardial necrosis. | | 1999 | 10327150 |
| chronic pure red cell aplasia caused by parvovirus b19 in aids: use of intravenous immunoglobulin--a report of eight patients. | the optimal management of chronic pure red cell aplasia caused by parvovirus b19 (b19-prca) in patients with aids is unclear. our purpose was to determine the effects of intravenous immunoglobulin (ivig) in the treatment of b19-prca in patients with aids. the patients were eight adults with aids admitted during the period 1993-1997. a diagnosis of b19-prca was made if all the following criteria were met: 1. bone marrow biopsy finding of pure red cell aplasia; 2. detection of parvovirus b19 dna i ... | 1999 | 10331506 |
| precise quantitation of human parvovirus b19 dna in biological samples by pcr. | an application of a quantitative pcr-based method was developed for the detection of human parvovirus b19 dna. the procedure was characterised according to guidelines for the validation of analytical procedures. furthermore, the reliability was demonstrated by the correct quantitation of samples of an international collaborative study. this application might be useful for studies focussed on removal and/or inactivation procedures of human parvovirus b19 as well as for general screening purposes ... | 1998 | 10336265 |
| [case of hereditary spherocytosis complicated by human parvovirus b19-associated hemophagocytosis syndrome and aplastic crisis]. | | 1999 | 10341571 |
| parvovirus b19 and systemic sclerosis. | | 1999 | 10342065 |
| solvent/detergent fresh frozen plasma as primary treatment of acute thrombotic thrombocytopenic purpura. | three patients with acute thrombotic thrombocytopenic purpura (ttp) (one associated with e. coli infection) received as primary treatment pooled fresh frozen plasma (ffp) virus inactivated by the solvent/detergent (sd) method, plus prednisolone. all three patients attained platelet counts of > 50 x 10(9)/l by days 7, 3 and 10, respectively. serum creatinine became corrected in parallel. neurological features were slow to resolve in one patient, and developed de novo during plasma infusion in ano ... | 1999 | 10342071 |
| parvovirus infection mimicking systemic lupus erythematosus in a pediatric population. | to assess the striking similarities of presentation in a pediatric population between human parvovirus b19 (hpv-b19) infection and systemic lupus erythematous (sle). | 1999 | 10342388 |
| human parvovirus b19 infection: its relationship with systemic lupus erythematosus. | the clinical presentation and outcome of four cases of human parvovirus-b19 (hpv-b19) infection, initially diagnosed as systemic lupus erythematosus (sle), were reviewed and compared with similar cases previously reported in the literature. the relationship between hpv-b19 infection and sle is discussed. | 1999 | 10342389 |
| rheumatoid arthritis after human parvovirus b19 infection. | | 1999 | 10343531 |
| parvovirus b19: twenty-five years in perspective. | | 1999 | 10347273 |
| [round table: urticaria in relation to infections]. | 1) to study the clinical and analytic features of infectious disease associated to urticaria in children. 2) to look into the probable etiology of the infectious disease. 3) to determine atopic predisposition and previous urticarial episodes and to rule out the involvement of antibiotics. | 1999 | 10354011 |
| [drug-induced papular-purpuric gloves and socks syndrome]. | the papular-purpuric "gloves and socks" syndrome (ppgss) was first described in 1990 by harms et al. the syndrome is characterised by fever, an itchy erythema with edema, confluent papules and purpura of the hands and feet in a "gloves and socks" distribution. the skin lesions may be associated with purpura and superficial erosions of the oral mucosa. viral infections, in particular parvovirus b19, are the most likely trigger. in the present case ppgss developed after taking trimethoprim/sulfame ... | 1999 | 10354921 |
| genetic heterogeneity of the immunogenic viral capsid protein region of human parvovirus b19 isolates obtained from an outbreak in a pediatric ward. | whereas human parvovirus b19 commonly infects children and causes erythema infectiosum, it causes more severe diseases when it infects adults. in order to examine whether different clinical outcomes of b19 infection can be ascribed to the viral genetic heterogeneity, we have determined the nucleotide sequence of highly immunogenic portions of the b19 genome obtained from six patients with various clinical manifestations in a single outbreak. our observations demonstrated that although the b19 se ... | 1999 | 10359091 |
| prenatal diagnosis of parvovirus b19-induced hydrops fetalis by chemiluminescence in situ hybridization. | parvovirus b19 can be transmitted transplacentally from the infected mother to the fetus during pregnancy, and hydrops fetalis, abortion, or stillbirth can result. in our study we explored the use of chemiluminescence in situ hybridization to detect b19 dna on cord blood cells, amniotic fluid cells, and pleuric fluid cells from several cases of hydrops fetalis. b19 dna was detected by using digoxigenin-labeled probes immunoenzymatically visualized with the chemiluminescent adamantil-1,2-dioxetan ... | 1999 | 10364606 |
| correlation between cytomegalovirus infection and raynaud's phenomenon in lupus nephritis. | relationships between viruses and autoimmune diseases such as systemic lupus erythematosus (sle) are still elusive. recent reports demonstrated the association of some viral infections with peculiar clinical events in the general population, such as cytomegalovirus (cmv) with arterial damage and parvovirus b19 (pv-b19) with hematologic abnormalities. we planned to look for this kind of viral imprinting in sle, hypothesizing that traces of specific features of some viral infections might be found ... | 1999 | 10364707 |
| the role of parvovirus b19 in the pathogenesis of giant cell arteritis: a preliminary evaluation. | to determine whether parvovirus b19 dna is more likely to be present in the temporal arteries of patients with giant cell arteritis (gca) than in the temporal arteries of control subjects. | 1999 | 10366119 |
| all cases of intrauterine fetal death should be evaluated for parvovirus b19 viral deoxyribonucleic acid. | | 1999 | 10368510 |
| clinical and laboratory findings in immunocompetent patients with persistent parvovirus b19 dna in bone marrow. | the clinical relevance of parvovirus b19 dna persistence in bone marrow was examined in 10 immunocompetent individuals undergoing examinations for unexplained fever, arthralgia or chronic leukopenia. common causes of these symptoms had been ruled out and bone marrow aspiration was indicated at this stage of investigation. in addition to morphological analysis of the bone marrow, a test for b19 dna was performed with 2 nested pcrs. five of these 10 selected patients had detectable b19 dna in thei ... | 1999 | 10381211 |
| surveillance of congenital rubella in great britain. rubella can be mistaken for parvovirus b19 infection. | | 1999 | 10390479 |
| human parvovirus b19 infection in multiple sclerosis. | the association of human parvovirus b19 (pvb19) infection with the development of several systemic autoimmune diseases has been confirmed in other studies. to determine if there is any association of pvb19 infection with multiple sclerosis (ms), we studied the prevalence of serum anti-pvb19 igg and igm by an enzyme-linked immunosorbent assay and pvb19-specific dna in the cerebrospinal fluid (csf) by the polymerase chain reaction method in a total of 46 patients during exacerbation or remission o ... | 1999 | 10394046 |
| prevalence of antibodies to human parvovirus b19 nonstructural protein in persons with various clinical outcomes following b19 infection. | persistent infections with human parvovirus b19 (b19) associated with debilitating chronic disease have been described, although evidence linking b19 to these more unusual clinical outcomes has been inconclusive. recent reports have suggested that the development of antibodies to the b19 nonstructural protein (ns1) following b19 infection might be linked to development of severe arthropathy and chronic infection. to confirm these findings, the c-terminal region of the ns1 protein was expressed f ... | 1999 | 10395869 |
| acute pure red cell aplasia associated with allopurinol therapy. | several investigators have reported patients with acute pure red cell aplasia (prca) caused by anticonvulsants, antibiotics, or antithyroid agents. allopurinol is known to be a causative agent of aplastic anemia, but there have been few reports of acute prca induced by allopurinol. we describe here a 15-year-old boy who suffered from anemia 6 weeks after initiation of allopurinol therapy; his anemia immediately improved after cessation of the drug. his bone marrow showed severe erythroid hypopla ... | 1999 | 10398315 |
| the simian parvoviruses. | autonomous parvoviruses with tropism for erythroid cells have recently been reclassified in a new genus, erythrovirus. although b19 is the type member, and presently the only internationally accepted member of the erythrovirus genus, we have identified three new simian viruses, all of which have the molecular features of parvoviruses, and are highly tropic for erythroid progenitor cells. this review describes the identification of these new animal parvoviruses and summarises current knowledge of ... | 1997 | 10398485 |
| human parvovirus b19: relevance in internal medicine. | infection by the human parvovirus b19 can lead to several clinical manifestations which are relevant in internal medicine. these include aplastic crisis in chronic haemolytic anaemias, exanthemathous disease and arthropathy, mainly in women, and chronic anaemia in the immunocompromised host. after initial replication, probably in the respiratory tract, the virus enters its target cells in the bone marrow, erythroid precursor cells, through its receptor, the blood group p antigen. viral replicati ... | 1999 | 10399450 |
| three-dimensional structure of aleutian mink disease parvovirus: implications for disease pathogenicity. | the three-dimensional structure of expressed vp2 capsids of aleutian mink disease parvovirus strain g (advg-vp2) has been determined to 22 a resolution by cryo-electron microscopy and image reconstruction techniques. a structure-based sequence alignment of the vp2 capsid protein of canine parvovirus (cpv) provided a means to construct an atomic model of the advg-vp2 capsid. the advg-vp2 reconstruction reveals a capsid structure with a mean external radius of 128 a and several surface features si ... | 1999 | 10400786 |
| novel human erythrovirus associated with transient aplastic anemia. | erythrovirus (formerly parvovirus) b19 causes a wide range of diseases in humans, including anemia due to aplastic crisis. diagnosis of b19 infection relies on serology and the detection of viral dna by pcr. these techniques are usually thought to detect all erythrovirus field isolates, since the b19 genome is known to undergo few genetic variations. we have detected an erythrovirus (v9) markedly different from b19 in the serum and bone marrow of a child with transient aplastic anemia. the b19 p ... | 1999 | 10405389 |
| parvovirus b19 infection in thoracic organ transplant recipients. | clinical manifestations of parvovirus b19 infection in immunocompromised patients are mostly reported as acute or chronic hematologic disorders. more recently, respiratory or renal involvement has been described. | 1999 | 10405890 |
| intrauterine anemia due to parvovirus b19: successful treatment with intravenous immunoglobulins. | | 1999 | 10406919 |
| the role of parvovirus b19 infection in childhood acute lymphoblastic leukemia. | the authors hypothesized that parvovirus b19 with its hematotropic effects has the potential to precipitate varying forms of cytopenia in patients prior to or at the diagnosis of acute lymphoblastic leukemia (all). consequently, and in view of the increasing number of cases reported, this retrospective study evaluated, for the first time, the possible role of parvovirus b19 infection in pediatric patients suffering from all, by investigating the frequency and clinical relevance of this infection ... | 1999 | 10407869 |
| viral arthritis. | identifying viral infections related to rheumatic syndromes and understanding the pathophysiologic mechanisms by which they cause disease are crucial steps to furthering our understanding of the pathogenesis of rheumatic disease. many common viral infections can induce autoantibody formation. parvovirus b19 (b19) can cause acute arthritis, and occasionally chronic arthropathy, in infected adults. persistent b19 infection can be found in synovium of some patients. antibodies reactive with b19 epi ... | 1999 | 10411381 |
| parvovirus b19 infection associated with acute hepatitis and a purpuric exanthem. | | 1999 | 10417538 |
| [prenatal diagnosis of viral infections. a two year study in strasbourg]. | we report here the results of a 2-year study on the prenatal diagnosis of viral infections in strasbourg. this screening was carried out by virus isolation, by pcr assay, or by detection of igm fetal antibody for 98 pregnant women at risk of transmitting one of the viruses that causes fetal disease such as parvovirus b19 (b19), herpesviruses [cytomegalovirus (cmv), varicella-zoster virus, herpes simplex virus] and rubella virus. a viral etiology was proven in 7 out 98 cases: pcr applied to b19 d ... | 1999 | 10418033 |
| role of parvovirus b19 infection in childhood idiopathic thrombocytopenic purpura. | although parvovirus b19 exhibits a strong tissue-tropism for erythroid progenitor cells leading to anaemia, several case reports indicate that parvovirus b19 infection may also cause the development of thrombocytopenia. despite recent studies, the frequency and clinical relevance of this association have remained questionable. consequently, and in view of the paucity of evidence regarding a viral aetiology for idiopathic thrombocytopenic purpura (itp), we examined the role of parvovirus b19 in 4 ... | 1999 | 10419244 |
| seroprevalence of anti-human parvovirus b19 antibodies in patients attending a centre for sexually transmitted diseases. | the aim of this study was to establish the serological prevalence of anti-human parvovirus b19 (hp-b19) antibodies in a group of 321 patients attending a centre for sexually transmitted diseases (stds) and epidemiologically examine whether this virus may also be sexually transmitted. for this purpose, the serum prevalence of anti-hp-b19 evaluated in std patients (39%) was compared with that of 164 healthy blood donors (10%, p < 0.001), using commercially available elisa methods detecting the ant ... | 1999 | 10423735 |
| the transfusion-associated transmission of parvovirus b19. | | 1999 | 10425692 |
| [imitation of an acute exacerbation of established systemic lupus erythematosus caused by parvovirus b19 infection]. | a 26-year-old woman, for 3 years known to have systemic lupus erythematodes (sle), was admitted because of fever, muscle and joint pains as well as nausea and vomiting. maintenance therapy of 50 mg azathioprine, three times daily, had been stopped by the patient several weeks earlier. physical examination was unremarkable except for pale skin and mucosae as well as pain on pressure over muscle groups of the upper arms and thighs and paravertebrally. | 1999 | 10432949 |
| prevalence of parvovirus b19 dna in bone marrow of patients with haematological disorders. | patients with haematological disorders (n = 100) were examined for prevalence of parvovirus b19 dna in the bone marrow and serum, irrespective of b19-related symptoms. b19 dna was studied using 2 nested pcrs and the serum samples were further analysed with b19-specific igg, igm and avidity as well as seroreactivity against linear and conformational epitopes of the b19 vp2 antigen. the latter assays specify whether the igg antibody response represents acute or past b19 infection. b19 dna was dete ... | 1999 | 10447317 |
| cardiorespiratory manifestations of parvovirus b19 infection. | | 1999 | 10451194 |
| parvovirus glomerulonephritis in a patient with sickle cell disease. | a child with sickle cell disease developed glomerulonephritis 10 days following an aplastic crisis induced by human parvovirus b 19 infection. an initial kidney biopsy showed focal proliferative glomerulonephritis, and 1 year later was compatible with focal and segmental glomerulosclerosis. renal tissue, tested by polymerase chain reaction, was positive for parvovirus, while the patient's blood was negative. for the first time a direct relationship has been demonstrated between parvovirus infect ... | 1999 | 10454787 |
| acute parvovirus infection in a heart transplant recipient. | | 1999 | 10456000 |
| the use of monoclonal antibody r92f6 and polymerase chain reaction to confirm the presence of parvovirus b19 in bone marrow specimens of patients with acquired immunodeficiency syndrome. | parvovirus b19 infection is a cause of chronic anemia and red cell aplasia in patients with acquired immunodeficiency syndrome (aids) and in other immunocompromised hosts. anemia in aids patients has a multifactorial etiology, with parvovirus b19 infection being an infrequent but nevertheless treatable cause. therapy with intravenous immune globulin can result in rapid improvement of parvovirus-induced anemia. this treatment is expensive, therefore accurate and rapid confirmation of parvovirus i ... | 1999 | 10458821 |
| standardization of a pcr-elisa in serum samples: diagnosis of active parvovirus b19 infection. | to standardize a pcr assay for the detection of parvovirus b19 dna in serum samples three different sample treatments were evaluated on the basis of the efficiency of recovery, reproducibility, convenience of sample handling, and presence of pcr inhibitors. moreover, the presence of an internal standard competitor as the working reagent at one defined concentration in a competitive pcr-elisa has been suggested as a valid tool to standardize and validate the assay. the results indicated that seru ... | 1999 | 10459163 |
| exonuclease-released fluorescence detection of human parvovirus b19 dna. | background: the 5;-->3;-exonuclease activity of thermus aquaticus (taq) dna polymerase permits polymerase chain reaction (pcr) product detection immediately after amplification using a fluorogenic probe. this approach eliminates the requirement for gel electrophoresis or enzyme immunoassays (eia). the ligonucleotide probe is labeled with a reporter dye at its 5' terminus and a quencher dye at its 3' terminus and is present during dna amplification. the exonuclease cleaves the reporter molecule f ... | 1996 | 10462579 |
| parvovirus b19 infection induces apoptosis of erythroid cells in vitro and in vivo. | intrauterine parvovirus b19 infection is related to non-immune hydrops fetalis, the pathogenesis of which is based on the strict tropism of b19 for erythroid precursor cells and the massive destruction of the infected erythroid cells, although the mechanism of beta19-induced cytotoxicity has not been studied in detail. the purpose of this study is to provide empirical evidence that beta19 induces apoptosis of erythroid cells both in vitro and ill vivo. | 1999 | 10468132 |
| development of a chemiluminescence competitive pcr for the detection and quantification of parvovirus b19 dna using a microplate luminometer. | quantitative pcr of viral nucleic acids can be useful clinically in diagnosis, risk assessment, and monitoring of antiviral therapy. we wished to develop a chemiluminescence competitive pcr (cpcr) for parvovirus b19. | 1999 | 10471640 |
| a new method with general diagnostic utility for the calculation of immunoglobulin g avidity. | the reference method for immunoglobulin g (igg) avidity determination includes reagent-consuming serum titration. aiming at better igg avidity diagnostics, we applied a logistic model for the reproduction of antibody titration curves. this method was tested with well-characterized serum panels for cytomegalovirus, epstein-barr virus, rubella virus, parvovirus b19, and toxoplasma gondii. this approach for igg avidity calculation is generally applicable and attains the diagnostic performance of th ... | 1999 | 10473525 |
| prolonged fever caused by parvovirus b19-induced meningitis: case report and review. | | 1999 | 10476760 |
| [parvovirus b19 infection]. | in 1975, during blood screening for hepatitis b, cossart et al. discovered the human parvovirus b19 (b19). it is a small, single strand dna virus of the parvoviridae family. this virus is widespread with 40-80% of adults showing evidence of infection. it is found in the respiratory secretions of viraemic patients and direct contact has been suggested as the most likely mode of transmission. parenteral transmission is common during treatment with clotting-factor concentrates, but rarely occurs du ... | 1999 | 10481322 |
| [lung and bone marrow granulomas associated with human parvovirus b19 infection]. | a 56-year-old man was admitted with flu-like symptoms, and his platelet count abruptly decreased. a chest x-ray film showed granular shadows, and lung and bone marrow specimens disclosed non-caseating epithelioid cell granulomas. the patient's serum igm titer for human parvovirus (hpv) b19 was elevated. our conclusion was that hpv b19 must be kept in mind as a possible pathogenic agent of granuloma formation. | 1999 | 10481468 |
| possible interactions between the ns-1 protein and tumor necrosis factor alpha pathways in erythroid cell apoptosis induced by human parvovirus b19. | human erythroid progenitor cells are the main target cells of the human parvovirus b19 (b19), and b19 infection induces a transient erythroid aplastic crisis. several authors have reported that the nonstructural protein 1 (ns-1) encoded by this virus has a cytotoxic effect, but the underlying mechanism of ns-1-induced primary erythroid cell death is still not clear. in human erythroid progenitor cells, we investigated the molecular mechanisms leading to apoptosis after natural infection of these ... | 1999 | 10482630 |
| [hereditary hemoglobin h disease in japanese siblings diagnosed by human parvovirus b19 infection]. | hereditary hemoglobin h (hbh) disease was diagnosed in 2 japanese sisters who presented with aplastic crisis following acute human parvovirus b19 (hpv b19) infection. the proband, an 8-year-old girl, developed persistent fever and pallor, and samples of her peripheral blood showed hypochromic microcytic anemia. other laboratory data were consistent with hemolytic anemia. fever and signs of hypochromic microcytic anemia also developed in her sister 9 days later. cation exchange hplc analysis of t ... | 1999 | 10483135 |
| chemiluminescence western blot assay for the detection of immunity against parvovirus b19 vp1 and vp2 linear epitopes using a videocamera based luminograph. | a chemiluminescent western blot (wb) assay was developed to detect the immune status against linear epitopes of parvovirus b19 structural proteins vp1 and vp2. the chemiluminescent wb assay combined the sensitivity of chemiluminescent substrates and the objective evaluation of the luminescent signal obtained with a new system which consists of a videocamera-based, high-performance, low light level imaging luminograph connected to a personal computer for image analysis. the potential for diagnost ... | 1999 | 10488766 |
| parvovirus b19 and the pathogenesis of rheumatoid arthritis: a case for historical reasoning. | | 1999 | 10501383 |
| possible evolution of human parvovirus b19 infection into erythroleukemia. | | 1999 | 10509050 |
| fetal brain infection with human parvovirus b19. | intrauterine parvovirus b19 infection is known to be one of the causes of hydrops fetalis. however, there are few reports of the pathologic changes in the central nervous system. postmortem examination of a fetus revealed multinucleated giant cells of macrophage/microglia lineage and many small calcifications around the vessels, predominantly in the cerebral white matter. parvovirus b19 genome dna was detected in the nucleus of the multinucleated giant cells and solitary endothelial cells by pol ... | 1999 | 10513695 |
| parvovirus b19 and schönlein-henoch purpura in adults. | | 1999 | 10516501 |
| prevalence of human parvovirus b19 dna and igg/igm antibodies in polish haemophilia patients. | | 1999 | 10516556 |
| parvovirus b19 infections. | infections caused by human parvovirus b19 can result in a wide spectrum of manifestations, which are usually influenced by the patient's immunologic and hematologic status. in the normal host, parvovirus infection can be asymptomatic or can result in erythema infectiosum or arthropathy. patients with underlying hematologic and immunologic disorders who become infected with this virus are at risk for aplastic anemia. hydrops fetalis and fetal death are complications of intrauterine parvovirus b19 ... | 1999 | 10524489 |
| [influence of specific clinical lessons in the dutch journal of medicine on clinical management]. | to establish whether publication of an article propagating a laboratory test leads to measurable increase of the number of requests for that test. | 1999 | 10526616 |
| pancytopenia with hemophagocytosis secondary to parvovirus b19 infection in a family with hereditary spherocytosis. | | 1999 | 10530073 |
| parvovirus b19 infection does not contribute significantly to severe anaemia in children with malaria in malawi. | | 1999 | 10530420 |
| papular-purpuric "gloves and socks" syndrome: polymerase chain reaction demonstration of parvovirus b19 dna in cutaneous lesions and sera. | we report a typical case of papular-purpuric "gloves and socks" syndrome (ppgss) in which primary infection by parvovirus b19 was demonstrated by seroconversion to this virus; parvovirus b19 dna was also identified by polymerase chain reaction (pcr) methods in the sera of the patient and in the cutaneous biopsy specimen, both taken 4 days after the onset of clinical manifestations. to our knowledge, this is the fourth published case in which parvovirus b19 dna has been recovered from the skin by ... | 1999 | 10534650 |
| [antibodies against human parvovirus b19 in the madrid community]. | | 1999 | 10535193 |
| one stillborn and one severely hydropic twin due to parvovirus b19 infection; successful outcome of the surviving twin. | | 1999 | 10535353 |
| human parvovirus b19 infection in acute fulminant liver failure. | we previously reported detection of human parvovirus b19 dna in livers from patients requiring transplantation for acute fulminant liver failure. in this study, we used immune adherence pcr (ia-pcr) to bind b19 virions in recipient native liver onto solid phase with specific monoclonal antibodies followed by pcr amplification of virion dna. ia-pcr had sensitivity and specificity similar to conventional pcr. we examined liver tissue from 16 patients with non-a, non-b, non-c, non-e (na-e) acute fu ... | 1999 | 10542021 |
| the prevalence of antibody to human parvovirus b19 in pregnant women in kuwait. | infection with parvovirus b19 during pregnancy is known to be associated with various fetal damage, such as aplastic anemia and hydrops fetalis. our objective was to study the seroprevalence of parvovirus b19 in the obstetric population in kuwait and to compare this with that in the adult population in other regions. blood samples from 1047 pregnant women were used in this prospective study. information regarding patient's age, parity, nationality and symptoms was obtained at the time of collect ... | 1999 | 10546839 |
| acute parvovirus b19 infection in connection with a flare of systemic lupus erythematodes in a female patient. | since its discovery parvovirus b19-infections could be linked to a growing variety of diseases. besides the harmless exanthema erythema infectiosum perferentially observed with b19-infections in childhood a panel of rather serious and also chronic courses that may be associated with anemia, thrombocytopenia, arthritis and others have been described. | 1999 | 10548133 |
| [parvovirus b19 as a cause of acute arthritis]. | parvovirus b19 is known to cause erythema infection (fifth disease), acute and chronic arthritis, aplastic crises in chronic hemolytic anemia, chronic anemia in the immunocompromised host and hydrops fetalis. we present two patients with acute arthritis due to parvovirus infection. both had symmetrical synovitis in wrists and ankles. patient 1 presented with fever and rash before joint symptoms occurred; patient 2 had joint symptoms only. arthritis due to parvovirus is usually self-limited, but ... | 1999 | 10553337 |
| prenatal diagnosis and management of nonimmune hydrops fetalis. | we examined the incidence, aetiological factors and outcome in 40 cases of nonimmune hydrops fetalis (nih) and suggest a rational approach to management. the incidence of nih was 1 in 830 deliveries during the last 10-year period. in spite of extensive antenatal and postnatal investigation no cause could be established in 14 (35%) cases. a probable aetiological factor was found in 65% of cases. these included viral infection (7), cardiovascular (6), twin-to-twin transfusion (3), chromosomal abno ... | 1999 | 10554935 |
| remission of juvenile rheumatoid arthritis after infection with parvovirus b19. | a 22-year-old caucasian woman with a 6 year history of persistently active, systemic onset juvenile rheumatoid arthritis (jra) developed symptoms of headache, dry cough, nausea, vomiting, abdominal pain, diarrhea, and dehydration associated with a high fever, elevated liver enzymes, and lymphopenia. subsequent investigation revealed acute infection with parvovirus b19. following clinical improvement over 10-14 days solely with supportive care, her underlying disease remained in remission for abo ... | 1999 | 10555914 |
| acute-phase-specific heptapeptide epitope for diagnosis of parvovirus b19 infection. | the major capsid protein vp2 of human parvovirus b19, when studied in a denatured form exhibiting linear epitopes, is recognized exclusively by immunoglobulin g (igg) antibodies of patients with acute or recent b19 infection. by contrast, conformational epitopes of vp2 are recognized both by igg of the acute phase and by igg of past immunity. in order to localize the vp2 linear epitope(s) specific for acute-phase igg, the entire b19 capsid protein sequence was mapped by peptide scanning using we ... | 1999 | 10565913 |
| seroprevalence of parvovirus b19 ns1-specific igg in b19-infected and uninfected individuals and in infected pregnant women. | parvovirus b19 is the causative agent of erythema infectiosum in children, but the virus is associated with an increasing range of different diseases. these include acute and chronic arthritis, hydrops fetalis in pregnant women, aplastic anemia, and thrombocytopenia. the host's immune response is directed against the viral structural proteins vp1 and vp2. this study investigated the presence of igg against the viral nonstructural protein ns1 using western blot. serum panels from healthy individu ... | 2000 | 10568763 |
| [12th congress of virology of versailles-le chesnay. 20, 21 may 1999]. | | 1999 | 10577288 |
| there is no association between polymyalgia rheumatica and acute parvovirus b19 infection. | | 1999 | 10577374 |
| parvovirus b19 infection causing red cell aplasia in renal transplantation on tacrolimus. | parvovirus b19 infection is known to cause chronic anemia in immunocompromised hosts, including organ transplant recipients. most reported cases of parvovirus b19-associated aplastic anemia in renal transplant recipients responded to intravenous immunoglobulin (ivig) infusion. tacrolimus is of special interest; it was proposed to be associated with pure red cell aplasia (prca) on its own because resolution of anemia on withdrawal of tacrolimus was previously observed. interaction between parvovi ... | 1999 | 10585325 |
| simultaneous igm reactivity by eia against more than one virus in measles, parvovirus b19 and rubella infection. | a clinical diagnosis of rash-causing infections is not always possible and reliance has to be placed on serological evidence of infection, especially on the presence of specific immunoglobulin (ig)m. however, despite the use of modern serological methods and validated commercial kits, reports appear in the literature of simultaneous igm reactivity against more than one virus in cases of epstein barr virus, rubella, cytomegalovirus, human parvovirus b19 (hpv b19) and measles infections, all with ... | 1999 | 10588453 |
| prenatal diagnosis of congenital parvovirus b19 infection: value of serological and pcr techniques in maternal and fetal serum. | intrauterine infection with parvovirus b19 (b19) is associated with non-immune hydrops fetalis, miscarriage and stillbirth. accurate laboratory tests for diagnosis of b19 infection are required to exclude other diagnoses. we analysed the diagnostic value of b19 igm antibody testing and polymerase chain reaction (pcr) in the sera from 57 patients and their fetuses with abnormal ultrasonography. viral dna was found in 16 of the 58 fetuses (one twin pregnancy) whereas only 7 of these were tested po ... | 1999 | 10590428 |
| [the role of genetic diagnosis in clinics--from the choice of ordering until reading the data]. | genetic diagnosis is a revolutionary method that makes possible simultaneous viral isolation (detection) and identification. the method is so specific, sensitive, and rapid (non-culture) that leads not only to the diagnosis of viral infection, but also to prediction of the chemotherapy, monitoring during the therapy, and judging the efficacy of the treatment. moreover, it contributes to understanding the disease pathophysiology. the qualitative results are sufficient for diagnosis, but quantitat ... | 1999 | 10590677 |
| efficacy of viral clearance methods used in the manufacture of activated prothrombin complex concentrates: focus on autoplex t. | various methods are described for the elimination of infectious viruses from activated prothrombin complex concentrates (apccs) and for the analysis of the final products (autoplex t and feiba vh). viruses of concern in human plasma-derived products are enveloped (hepatitis b and c, cytomegalovirus, epstein-barr virus, and human immunodeficiency virus [hiv]) and nonenveloped (hepatitis a and parvovirus b19). donated blood used for autoplex t is screened for antihepatitis c, hbsag, anti-hiv types ... | 1999 | 10597384 |
| infection of the erythroid cell line, ku812ep6 with human parvovirus b19 and its application to titration of b19 infectivity. | a human parvovirus b19 (b19) infectivity assay was developed using the erythroid cell line, ku812ep6. ku812ep6 was cloned for high efficiency infection with b19 in vitro, in the presence of erythropoietin by a limiting dilution method from the parent cell line, ku812. b19 was effectively propagated in ku812ep6 and was detected for b19 antigens, vp1 and vp2. the titers of b19 positive sera measured with ku812ep6 cells were in the range of 10(6) to 10(8) tcid50 ml. this ku812ep6 infectivity assay ... | 1999 | 10598082 |
| pattern of parvovirus b 19 infection during different trimesters of pregnancy in kuwait. | aims of this study were to determine the igg and igm seropositivity to parvovirus b19 during the three trimesters of pregnancy. | 1999 | 10598918 |
| a randomized trial of solvent/detergent-treated and standard fresh-frozen plasma in the coagulopathy of liver disease and liver transplantation. | virus inactivation of pooled fresh-frozen plasma (ffp) by the solvent/detergent (sd) method results in a loss of approximately 20 percent of factor viii. this study aimed to assess the efficacy of sd-treated plasma in correcting the coagulopathy associated with liver disease and liver transplantation. | 1999 | 10604250 |
| the spectrum of cutaneous eruptions in 22 patients with isolated serological evidence of infection by parvovirus b19. | | 1999 | 10606079 |
| [parvovirus b19 infection--an insidious chameleon]. | parvovirus b19 is a common source of infection with a seroprevalence of 60-70 per cent in the adult population. the most common manifestation is erythema infectiosum ('fifth disease'), with exanthem, fever and upper airway symptoms in children. the infection can give rise to a multifacetted clinical picture and is probably underdiagnosed, particularly in risk groups (individuals with haemolytic anaemia or immunosuppression, and fetuses). serological diagnosis can now be complemented with the dem ... | 1999 | 10608130 |
| parvovirus b19 infection of bone marrow in systemic sclerosis patients. | to investigate the prevalence of human parvovirus b19 (b19) infection in the bone marrow of systemic sclerosis (ssc) patients. | 1999 | 10609071 |