| natural killer-mediated lysis of some but not all hsv-1- or vsv-infected targets requires the participation of hla-dr-positive accessory cells. | natural killer (nk)-mediated lysis of herpes simplex virus type 1-infected fibroblasts (hsv-fs) has been previously shown to require the co-operation of cd16-positive nk cells and an hla-dr-positive accessory cell population. in contrast, lysis of k562 tumour cells requires the presence of only the leu-11-positive cells. in the current study, targets of different morphologies, both virally infected and non-infected, were tested in an attempt to dissect out which target characteristics determine ... | 1991 | 1851136 |
| role of herpes simplex virus thymidine kinase expression in viral pathogenesis and latency. | herpes simplex virus (hsv) thymidine kinase (tk) expression and the hsv tk gene have been evaluated in studies of gene control, as well as in animal and human studies of viral pathogenesis, including hsv latency. in investigations of the biological role of hsv tk, enzyme expression was noted to be important for hsv infection of nonreplicating cells in culture; and, in experimental animal studies, hsv tk was shown to be important for in vivo latent infection of sensory ganglion neurons. latency i ... | 1991 | 1851146 |
| application of polymerase chain reaction assays to studies of herpes simplex virus latency. | we have amplified herpes simplex virus type 1 (hsv-1) dna sequences from individual latently infected mouse trigeminal ganglia by polymerase chain reaction (pcr) assays. this report presents two useful modifications in the pcr technique. the first involves the use of two sets of closely spaced, oppositely oriented oligonucleotide primers and two rounds of 20-40 pcr cycles, first with the more widely spaced outer primers and then with the internal nested primers. this method enhanced the sensitiv ... | 1991 | 1851147 |
| cell-mediated immunologic responses and recurrent genital herpes in the guinea pig. effects of glycoprotein immunotherapy. | the specific immune alterations associated with hsv recurrences are ill defined although it appears that alterations in cell-mediated immune mechanisms are more likely associated with recurrent disease than humoral immunity. immunization with hsv glycoproteins b and d (gbgd) after primary hsv infection has reportedly reduced the frequency of recurrences but the mechanisms remain unidentified. we therefore evaluated the effects of immunization with cloned gbgd on selected cell-mediated immune res ... | 1991 | 1851194 |
| in vitro photodynamic inactivation of herpes simplex virus with sapphyrins: 22 pi-electron porphyrin-like macrocycles. | the photodynamic inactivation of hsv-1, a virus having a membranous envelope, with both a decaalkyl sapphyrin and its dicarboxy-substituted analog was studied. the decaalkyl sapphyrin was as efficient in the inactivation of hsv-1 on a per macrocycle basis as dhe, whereas the efficiency of the dicarboxy-substituted sapphyrin was approximately two orders of magnitude less. fluorescence studies of sapphyrin's binding to liposomes and vsv suggested that the decaalkylsapphyrin bound monomerically to ... | 1991 | 1851302 |
| modulation by thiols of the merocyanine 540-sensitized photolysis of leukemia cells, red cells, and herpes simplex virus type 1. | this paper reports on the role of endogenous and exogenous thiols in the merocyanine 540 (mc 540)-sensitized photoirradiation of l1210 leukemia cells, human erythrocytes, and human herpes simplex virus type 1. several measures taken to decrease the intracellular content of glutathione enhanced the cells' sensitivity to mc 540-sensitized photoirradiation while stimulation of glutathione biosynthesis or supplementation of the extracellular or extraviral thiol content decreased the photosensitivity ... | 1991 | 1851305 |
| experimental therapy of human glioma by means of a genetically engineered virus mutant. | malignant gliomas are the most common malignant brain tumors and are almost always fatal. a thymidine kinase-negative mutant of herpes simplex virus-1 (dlsptk) that is attenuated for neurovirulence was tested as a possible treatment for gliomas. in cell culture, dlsptk killed two long-term human glioma lines and three short-term human glioma cell populations. in nude mice with implanted subcutaneous and subrenal u87 human gliomas, intraneoplastic inoculation of dlsptk caused growth inhibition. i ... | 1991 | 1851332 |
| detection of herpes simplex virus thymidine kinase and latency-associated transcript gene sequences in human herpetic corneas by polymerase chain reaction amplification. | herpes simplex virus (hsv) latency in sensory ganglion neurons is well documented, but the existence of extraneuronal corneal latency is less well defined. to investigate the possibility of extraneuronal latency during ocular hsv infection, corneal specimens from 18 patients with quiescent herpes simplex keratitis (hsk) were obtained at the time of keratoplasty. polymerase chain reaction (pcr) amplification followed by southern blot hybridization with a radiolabeled oligonucleotide probe was don ... | 1991 | 1851732 |
| (s)-1-(3-hydroxy-2-phosphonyl-methoxypropyl)cytosine in the therapy of thymidine kinase-positive and -deficient herpes simplex virus experimental keratitis. | the phosphonylmethoxyalkyl derivative, (s)-1-(3-hydroxy-2-phosphonyl methoxypropyl)cytosine (hpmpc), was evaluated for its efficacy in the topical treatment of experimental keratitis caused by thymidine kinase-positive (tk+) or thymidine kinase-deficient (tk-) herpes simplex virus type 1 (hsv-1) strains. the hpmpc 0.2% eyedrops were as effective as the reference compound, (e)-5-(2-bromovinyl)-2'-deoxyuridine, (bvdu) 0.2% eyedrops in stimulating the healing of epithelial disease caused by the hsv ... | 1991 | 1851733 |
| affinity purification of active subunit 1 of herpes simplex virus type 1 ribonucleotide reductase exhibiting a protein kinase activity. | herpes simplex virus (hsv) ribonucleotide reductase is formed by the association of two distinct dimeric subunits, r1 and r2. attempts to purify either the hsv holoenzyme or its r1 subunit in their active form have been unsuccessful until now. the c terminus of the r2 protein being involved in the association with r1, the synthetic nonapeptide corresponding to this terminus, impedes the formation of the holoenzyme by competing with r2 for a critical site on r1. based upon these observations, we ... | 1991 | 1851753 |
| activation of gene expression by human herpesvirus 6 is reporter gene-dependent. | infection with human herpesvirus 6 (hhv-6) was found to up-regulate expression of human immunodeficiency virus and human t cell leukaemia virus type i (htlv-i) long terminal repeat sequence (ltr), and herpes simplex virus type 1 (hsv-1) gd chloramphenicol acetyltransferase (cat) constructs transfected into the t cell line, j. jhan. activation by hhv-6 was due to one or more viral proteins produced early in infection and, in the case of the htlv-i ltr, was synergistic to induction mediated by the ... | 1991 | 1851812 |
| binding of herpes simplex virus to cellular heparan sulphate, an initial step in the adsorption process. | it has been suggested that heparan sulphate has a receptor function in the initial phase of the attachment of herpes simplex virus (hsv) to cells. we have studied the influence of glycosaminoglycans on cell adsorption of, and plaque formation by, hsv-1 and hsv-2, with regard to the role of saccharide structure, chain length and charge density. heparin and highly-sulphated heparan sulphate (1.5 sulphate groups/disaccharide unit), but neither chondroitin sulphate nor dermatan sulphate, were found ... | 1991 | 1851813 |
| inverted repeat regions of marek's disease virus dna possess a structure similar to that of the a sequence of herpes simplex virus dna and contain host cell telomere sequences. | the genomic structure of marek's disease virus (mdv) is similar to those of the alphaherpesviruses herpes simplex virus (hsv) types 1 and 2. sequence analysis of the junction region between the long component (l) and the short component (s) revealed the existence of an a-like sequence, similar in structure to the a sequence of hsv-1. further study revealed that the mdv genome contains five copies of the a-like sequence within the long terminal repeat region as well as in the short terminal repea ... | 1991 | 1851854 |
| a 269-amino-acid segment with a pseudo-leucine zipper and a helix-turn-helix motif codes for the sequence-specific dna-binding domain of herpes simplex virus type 1 origin-binding protein. | the ul9 gene of herpes simplex virus (hsv) codes for a dna-binding protein (obp) that interacts sequence specifically with the origin of replication. this protein is essential for hsv dna replication in cultured cells. the ul9 gene was cloned into a plasmid vector downstream of the sp6 rna polymerase promoter. by using in vitro transcription and translation systems, a full-length obp was synthesized. this synthetic protein is recognized by an antiserum generated against the c-terminal decapeptid ... | 1991 | 1851856 |
| expression of the herpes simplex virus 1 alpha transinducing factor (vp16) does not induce reactivation of latent virus or prevent the establishment of latency in mice. | a feature of the cascade regulation of herpes simplex virus 1 gene expression in productive infection is that the first genes to be expressed, the alpha genes, are transactivated by a structural component of the virion designated as the alpha transinducing factor (alpha tif). in this study, we have tested the hypothesis that latent infection of sensory neurons results from the failure of alpha tif, a tegument protein, to be transported from the nerve endings to the nucleus of the sensory neuron. ... | 1991 | 1851865 |
| herpes simplex virus type 1 origin-specific binding protein: oris-binding properties and effects of cellular proteins. | the herpes simplex virus type 1 (hsv-1) origin of replication, oris, contains three highly homologous sequences, sites i, ii, and iii. the hsv-1 origin-binding protein (obp), the product of the ul9 gene, has been shown to bind specifically to sites i and ii. in this study, gel shift analysis was used to characterize interactions between site i dna and proteins in infected and uninfected cell extracts. the formation of two protein-dna complexes, bands a and b, was demonstrated with infected cell ... | 1991 | 1851874 |
| the effect of hsv multiplication rate on antiviral drug efficacy in vitro. | hsv-1 multiplication rates have been shown to vary in different tissues and the rate of multiplication may correlate with susceptibility to antiviral chemotherapy. herpetic stromal keratitis is a necrotizing condition refractive to antiviral therapy and this lack of antiviral efficacy in stromal disease may be the result of very low rates of viral replication in the corneal stromal keratocytes. in this study, we investigated the efficacy of antiviral drugs in an in vitro system in which the viru ... | 1991 | 1852150 |
| differential suppression of host cell protein synthesis and mrna levels in herpes simplex virus-infected endothelial cells. | earlier studies from this laboratory have shown that infection of vascular cells with herpes simplex virus 1 or 2 (hsv-1, hsv-2) results in the differential suppression of extracellular matrix proteins including fibronectin (fn), type iv collagen, thrombospondin (tsp) and factor viii von willebrand protein. the present study was designed to determine whether a correlation exists between suppression of synthesis of specific proteins and their mrna levels. we have measured the steady-state levels ... | 1991 | 1891960 |
| conserved domains of glycoprotein b (gb) of the monkey virus, simian agent 8, identified by comparison with herpesvirus gbs. | the herpesvirus simian agent 8 (sa8) gene which corresponds to the herpes simplex virus (hsv) gene encoding glycoprotein b (gb) was localized, cloned and sequenced. comparison of its deduced amino acid sequence with those of its counterparts in 12 other distinct herpesvirus was used to evaluate their homology and phylogenetic relationship. the results emphasized that sa8 gb is more closely related to those of hsv-1 and -2, and bovine herpesvirus 2 than to the homologous proteins of other herpesv ... | 1991 | 1895066 |
| selective activity and cellular pharmacology of (1r-1 alpha,2 beta,3 alpha)-9-[2,3-bis(hydroxymethyl)cyclobutyl]guanine in herpesvirus-infected cells. | the cycloburtane nucleoside analog (1r-1 alpha,2 beta,3 alpha)-9-[2,3-bis(hydroxymethyl)cyclobutyl]guanine [(r)-bhcg or sq 34,514] was recently synthesized and shown to be the active enantiomer of (+/-)-bhcg (sq 33,054), a potent inhibitor of several strains of herpesviruses [j. med. chem 34:1415-1421 (1991); antiviral res. 13:41-52 (1990)]. in plaque reduction assays, (r)-bhcg was about 1000 times more active than its s-enantiomer on herpes simplex virus type i (hsv-1) and over 200 times more a ... | 1991 | 1896029 |
| delivery of ultraviolet-inactivated 35s-herpesvirus across an osmotically modified blood-brain barrier. | the present studies were undertaken to determine if viral particles can be delivered across the rat blood-brain barrier (bbb). osmotic bbb modification with intracarotid mannitol (25%) was immediately followed by bolus intracarotid administration of 0.5 ml purified, ultraviolet-inactivated, herpes simplex virus type 1 endogenously labeled with 35s-labeled methionine (2.0 x 10(6) cpm, approximately 5 x 10(8) plaque-forming units/ml). after 60 minutes, intravascular virus was cleared by saline per ... | 1991 | 1899695 |
| transforming potential is detectable in arteriosclerotic plaques of young animals. | the carcinogen-treated cockerel is a model for studying the early stages of arteriosclerotic plaque development. carcinogen administration accelerates arteriosclerotic plaque development in cockerels, and transforming elements are present in dna from advanced human plaques. in this study, we asked whether transforming elements could also be detected at early stages of plaque development in cockerels. nih3t3 cells were transfected with dna from plaques isolated from carcinogen-treated cockerels a ... | 1991 | 1905951 |
| alterations in oral microflora and pathogenesis of acute oral infections during remission-induction therapy in patients with acute myeloid leukaemia. | to investigate changes in the aerobic and facultatively anaerobic oral microflora during remission-induction chemotherapy in patients with acute myeloid leukaemia, 10 consecutive patients were studied during a period of 28 days. during antineoplastic treatment, the concentration of microorganisms in saliva doubled from day 0 to day 2, presumably as a result of a concurrent 64% decrease in the salivary flow rate. no changes in the relative proportion of individual microorganisms or acquisition of ... | 1991 | 1909053 |
| viruses and oral cancer. | oral cancer is a disease with a complex etiology. there is evidence for important roles of smoking, drinking, and genetic susceptibility, as well as strong indications that dna viruses could be involved. the herpes simplex virus type 1 has been associated with oral cancer by serological studies, and animal models and in vitro systems have demonstrated that it is capable of inducing oral cancer. papillomaviruses are found in many oral cancers and are also capable of transforming cells to a malign ... | 1991 | 1912147 |
| [an experimental study on the primary cell culture of human trigeminal ganglion]. | a primary cell culture of human fetal trigeminal ganglion in vitro was first established. the growth features and morphology of the three types of cells in the trigeminal ganglion were described. the neurons in the culture were identified with the immunoperoxidase technique. a new model for studying the latent infection of herpes simplex virus type 1 in human is provided. | 1991 | 1914711 |
| anti-glycoprotein d monoclonal antibody protects against herpes simplex virus type 1-induced diseases in mice functionally depleted of selected t-cell subsets or asialo gm1+ cells. | passive transfer of a monoclonal antibody (mab) specific for glycoprotein d (gd) is highly effective in preventing the development of herpes simplex virus type 1-induced stromal keratitis. in the present study, we investigated whether animals which had been functionally depleted of t-cell subsets or asialo gm1+ cells would continue to be responsive to mab therapy. balb/c mice were depleted of cd4+, cd8+, or asialo gm1+ cells by treatment with anti-l3t4, anti-lyt 2.2, or anti-asialo gm1 antibodie ... | 1991 | 1920624 |
| binding of general transcription factor tfiib to an acidic activating region. | a central issue in eukaryotic transcriptional regulation is the mechanism by which promoter-specific transcription factors (activators) stimulate transcription. two lines of evidence indicate that the general transcription factor tfiib is a pivotal component in the mechanism by which an acidic activator functions. first, during assembly of the preinitiation complex tfiib binding is a rate-limiting step enhanced by an acidic activator. second, the tfiib activity in a hela cell nuclear extract is ... | 1991 | 1922364 |
| [plants as a source of research in antiviral activity. example of the haemanthus albiflos bulb]. | the antiviral potency of an hydroalcoholic extract from haemanthus albiflos (amaryllidaceae) bulb was investigated. experimentations were conducted on continuous cell lines (bgm, ma 104, hep 2) seeded in microplates. three viruses from the rna group (poliovirus type i, vesicular stomatitis virus type 11 and simian rotavirus sa 11) and two from the dna group (adenovirus type 5, herpes simplex virus type 1) were tested. important reduction in yield of viral infectivity was observed with the rna gr ... | 1991 | 1933468 |
| mhc ii-restricted, cd4+ cytotoxic t lymphocytes specific for herpes simplex virus-1: implications for the development of herpetic stromal keratitis in mice. | herpetic stromal keratitis (hsk) appears to represent an immunopathological reaction in which cd4+ t cells play a prominent role. however, the exact immunopathological mechanism(s) utilized by cd4+ t cells during hsk remains to be elucidated. in this study, the presence of cytotoxic cd4+ t lymphocytes in the cervical and retropharyngeal lymph nodes of balb/c mice experiencing hsk was investigated. after in vitro depletion of cd4+ or cd8+ t cells with specific monoclonal antibodies and complement ... | 1991 | 1934628 |
| simian agent 8--a herpes simplex-like monkey virus. | this review summarizes the most recent information on simian agent 8, a herpes simplex-like monkey virus. the agent has a broad host range and--besides the classical morphogenesis (budding at the internal nuclear membrane)--the virus gets enveloped at all cytoplasmic membranes including the plasma membrane; strikingly it carries a rather prominent tegument. regarding its sequence arrangement sa8 can be grouped to the e-type genomes. it has a g + c-content of 69% and a total dna-homology with hsv ... | 1991 | 1934999 |
| structurally homologous ligand binding of integrin mac-1 and viral glycoprotein c receptors. | three spatially distant surface loops were found to mediate the interaction of the coagulation protein factor x with the leukocyte integrin mac-1. this interacting region, which by computational modeling defines a three-dimensional macromotif in the catalytic domain, was also recognized by glycoprotein c (gc), a factor x receptor expressed on herpes simplex virus (hsv)-infected endothelial cells. peptidyl mimicry of each loop inhibited factor x binding to mac-1 and gc, blocked monocyte generatio ... | 1991 | 1957171 |
| management of genital herpes simplex infection. | herpes simplex infection of the genitals is a common condition, more often due to herpes simplex virus (hsv) type 2 than to type 1 virus. there is a severe first attack followed by mild recurrences which are more common and more frequent after hsv-2 than after hsv-1 genital infection. clinical features with prodrome, vesicles and erosions may be characteristic allowing rapid clinical diagnosis. when possible laboratory confirmation should be attempted. general management includes simple hygiene, ... | 1991 | 1958714 |
| expression of human immunodeficiency virus type 1 gag gene using genetically engineered herpes simplex virus type 1 recombinants. | infectious herpes simplex virus type 1 (hsv-1) recombinants were constructed by inserting the cdna sequence of the human immunodeficiency virus type 1 (hiv-1) gag gene (from nucleotide position 675 [saci] to 3859 [asp718] of the cdna sequences of hiv-1 strain bh-10) within the dna sequences of the bamhi dna fragment b of the genome of an apathogenic hsv-1 strain hfem. this hsv-1 strain possesses a 4.1-kbp deletion within the bamhi dna fragment b between 0.762 and 0.789 map units of the viral gen ... | 1990 | 1962977 |
| regulation of expression of the glycoprotein genes of herpes simplex virus type 1 (hsv-1). | | 1990 | 1963032 |
| pathogenesis and latency of herpes simplex virus type 1 (hsv-1): an ophthalmologist's view of the eye as a model for the study of the virus-host relationship. | | 1990 | 1963036 |
| the t-lymphocyte response to varicella-zoster viral proteins. | | 1990 | 1963047 |
| active hsv-1 immunization prevents the cocarcinogenic activity of hsv-1 in the oral cavity of hamsters. | previous investigations have demonstrated that herpes simplex virus (hsv) increased the oral carcinogenic activity of 7,12-dimethylbenz[a]anthracene (dmba) probably by enhancing the dmba-induced amplification and overexpression of c-erb-b-1 proto-oncogene in hamster buccal pouch epithelium. the present study investigated the effect of active type 1 hsv (hsv-1) immunization on the development of oral cancer induced by hsv-1 and dmba, alone or in combination, in the hamster buccal pouch. the resul ... | 1990 | 1963216 |
| synthesis and antiviral activities of carbocyclic oxetanocin analogues. | 9-cyclobutyladenine (4a), cis- and trans-9-[3- (hydroxymethyl)cyclobutyl]adenine (4b) and 9-[3,3-bis(hydroxymethyl)cyclobutyl]adenine(4d) were prepared from the corresponding cyclobutylamine derivatives (1a, 1b and 1d). guanine congeners (9a, cis- and trans-9b and 9d) and carbocyclic oxetanocin g (1',2'-trans-9f) were also prepared. carbocyclic oxetanocin a(1',2'-trans-4f), the preparation of which we have already published, and g were found to be active against herpes simplex virus (type 1 and ... | 1990 | 1963811 |
| recurrent herpetic keratitis: failure to detect herpes simplex virus infection using the syva microtrak hsv1/hsv2 direct specimen identification/typing test. | a 35-year-old man had developed recurrent herpetic keratitis characterized by dendritic keratitis at intervals of a year. we were able to culture cytopathic agents repeatedly from his lesions by inoculating vero cells. the cultures yielded definitive evidence of a virus that caused a cytopathic effect within 3 days. however, these virus strains could not be identified as herpes simplex virus (hsv) in immunofluorescence assays using the syva microtrak hsv1/hsv2 direct specimen identification/typi ... | 1990 | 1963928 |
| [broncho-alveolar lavage in hiv-1 seropositive children]. | sixteen bronchoalveolar lavages (bal) were performed in 15 children with hiv1 seropositivity, 12 of them being infected by hiv1. bal was performed during episodes of acute pneumonitis with respiratory distress (group i: three cases) or without severity (group ii: five cases), or in the presence of asymptomatic radiological pulmonary abnormalities (group iii: seven cases). a specific diagnosis of infection was obtained in five cases of acute pneumonitis and 12 micro-organisms were identified by b ... | 1990 | 1963941 |
| antiviral activity of an extract of cordia salicifolia on herpes simplex virus type 1. | a partially purified extract (col 1-6) from whole plant of cordia salicifolia showed an inhibitory effect on herpes simplex virus type 1 (hsv-1). the activity of col 1-6 on different steps of hsv-1 replication in hela cells was investigated. under single-cycle replication conditions, col 1-6 exerted a greater than 99.9% inhibition in virus yield when added to the cells 3 h or 1.5 h before infection, and even when added 8 h after infection the extract still caused a greater than 99% inhibition. t ... | 1990 | 1963951 |
| the effect of indomethacin, prostaglandin e2 and interferon on the multiplication of herpes simplex virus type 1 in human lymphoid cells. | the control of latency and reactivation of herpesvirus (hsv) infection is poorly understood. the activation of latent hsv is usually associated with a local or systemic rise in prostaglandins (pgs). it is possible that pgs may act indirectly by suppressing the inhibitory effect of interferon (ifn) on hsv replication. ifn has also been shown to decrease the number of herpetic recurrences and to speed up the healing of lesions. we investigated the effect of indomethacin (ind: a non-steroid anti-in ... | 1990 | 1964373 |
| incidence of herpes simplex virus type-1 and type-2 from patients with primary (first-attack) genital herpes in sheffield. | in a study to determine the herpes simplex virus (hsv) type isolated from patients with primary (first-attack) genital herpes and to identify any clinical or epidemiological features that might influence the reported incidence of any particular viral type, there was almost an equal incidence of hsv-1 and hsv-2 isolated from women (48% versus 52%). in the case of women, the initial clinical diagnosis was correct in 97% with hsv-1 and 95% with hsv-2 infection. amongst men, hsv-2 was isolated more ... | 1990 | 1964600 |
| clinical and laboratory study of first episode genital herpes in singapore. | a prospective study of the epidemiology, clinical aspects and laboratory features of first episode genital herpes was carried out in middle road hospital, singapore between june 1986 and december 1987. a total of 68 patients were studied. there were more women than men in the study population with a female to male ratio of 7.5 to 1. the peak incidence of first episode genital herpes was between 20 and 29 years of age. specimens from 63 patients yielded positive culture for herpes simplex virus ( ... | 1990 | 1964601 |
| [antiviral activity of commercial immunoglobulin preparations]. | immunoglobulin preparations for intravenous use of five different firms--biotest, hoechst, merieux, sandoz, wwss--were used for the study. antibody level for epstein-barr, cytomegalia, herpes simplex, varicella-zoster and measles viruses was determined in these preparations stored at 4 degrees c and in order to determine their stability they were tested after incubation at 37 degrees c and 61 degrees c. the influence of immunoglobulin (bioglobulin and sandoglobulin) on mouse survival infected wi ... | 1990 | 1964995 |
| rapidly progressive acute retinal necrosis secondary to herpes simplex virus, type 1. | a systemically healthy 22-year-old man presented with unilateral acute retinal necrosis (arn) that featured diffuse retinal whitening throughout the posterior retina, exudative retinal detachment, and a visual acuity of no light perception. diagnostic vitrectomy revealed necrotic retinal cells containing intranuclear inclusions visible with light microscopy. on electron microscopy, viral particles consistent with a herpes family virus were detected. culture of the vitrectomy specimen showed herp ... | 1990 | 1965022 |
| influence of hyperthermia on experimental viral infections in vitro. | the effects of hyperthermia (39 and 41 c) on virus multiplication in vitro (hsv-1, vsv on vero cells and emc on l929 cells) have been explored. the cells were infected with hsv-1, vsv or emc viruses and heated to 39 or 41 c before, during or after the viral infection. the titer of the virus show that temperature of 41 c acts as an inhibitory agent on hsv-1 and vsv virus replication in vero cells. | 1990 | 1965095 |
| effect of 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil against herpes simplex virus type 1 infection in immunosuppressed mice. | 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-arau) reduced the mortality rates of (i) 7-week-old, cyclophosphamide-treated, immunosuppressed mice (cyp mice) intraperitoneally infected with a moderately virulent strain of herpes simplex virus type 1 and (ii) 4-week-old cyp mice infected with a less virulent strain at doses of 20 and 50 mg/kg of body weight twice daily and 5 mg/kg, respectively. the degree of efficacy of bv-arau was equivalent to that of acyclovir in 4-week-old cyp mice ... | 1990 | 1965108 |
| male-specific beta-cell dysfunction and diabetes resulting from increased expression of a syngeneic mhc class i protein in the pancreata of transgenic mice. | it is well established that insulin-dependent diabetes (iddm) is an autoimmune disease with a strong genetic link to the hla locus. it is less well understood, however, how the destruction of the insulin-producing beta cells is effected and why neighboring non-beta islet cells are spared. also incompletely explained are the observations that, unlike other autoimmune diseases such as multiple sclerosis, iddm does not preferentially affect females, the incidence of the disease is highest among you ... | 1990 | 1965148 |
| inducible expression of herpes simplex virus type 1 glycoprotein c in nih 3t3 cells. | antibodies directed against the glycoprotein c (gc) of herpes simplex virus type 1 (hsv-1) are known to be mainly hsv-1-specific, whereas antibodies against other major hsv-1 glycoproteins cross-react with hsv-2 antigens. to investigate the immunological features of gc-1, the gene encoding gc-1 was isolated and cloned from dna of cells infected with hsv-1. the 3.6 kbp sali fragment r of hsv-1 dna was modified in order to place the gene under transcriptional control of the glucocorticoid dependen ... | 1990 | 1965287 |
| antiviral potencies of bv-arau and related nucleoside analogues against varicella-zoster virus in different cell lines. | 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-arau) and nine other antiherpesviral nucleoside analogues were compared for their potencies against four strains of varicella-zoster virus (vzv) on three different cell lines: hel cells, vero cells, and ms cells established from a human malignant schwannoma. in contrast to the activity against herpes simplex virus type 1 previously reported, bv-arau showed extremely marked antiviral activity against vzv even on vero cells. ed50, 50% plaque r ... | 1990 | 1965323 |
| isolation and typing of herpes simplex viruses in the infections of mouth, respiratory and genital tracts. | a total of 60 herpes simplex virus (hsv) isolates obtained from individuals with genital herpes lesions in the mouth or respiratory illness were typed in this study. the hsv isolation rate in genital herpes was 31%. the typing was done by a direct fluorescent monoclonal antibody technique using commercial reagents. all the 60 vero cell grown isolates were successfully typed without any indeterminate results. nine isolates were typed as hsv 1 and 51 as hsv 2. the technique was easy to perform and ... | 1990 | 1965431 |
| serum and cerebrospinal fluid herpes simplex virus type 1 immunoglobulin g and m titers in four cases of herpes simplex encephalitis. | herpes simplex virus type 1 (hsv-1) igg and igm elisa titers were serially determined in serum and cerebrospinal fluid (csf) samples from 4 patients with hsv-1 encephalitis during a follow-up period of 1-26 months. in 3 out of 4 patients hsv-1 igm titers raised in csf during the acute phase of disease, thus allowing differentiation between primary and reactivated forms of hsv-1 encephalitis. hsv-1 igg titers showed a sharp elevation earlier in serum than in csf. specific igg index documented a l ... | 1990 | 1966065 |
| specific activation of b-cell clones within the central nervous system in course of herpes simplex encephalitis. | total and virus-specific immunoglobulin (ig) g oligoclonal bands were studied in paired serum and cerebrospinal fluid (csf) of four patients with herpes simplex virus type 1 (hsv-1) encephalitis. we used the isoelectric focusing in agarose gel, a sensitive technique for protein separation, followed by passive transfer of proteins on nitrocellulose paper and specific immunostaining. oligoclonal bands were observed in serum and csf of all patients. hsv-1-specific oligoclonal igg bands were present ... | 1990 | 1966066 |
| antibody to herpes simplex virus type 1 in peptic ulcer patients. | it has been postulated that herpes simplex virus type 1 may be a causative factor of duodenal ulcer. serum antibody titres to herpes simplex virus type 1 in duodenal and gastric ulcer patients were compared with race-, sex- and age-matched controls. no differences in antibody titres could be demonstrated between duodenal ulcer and gastric ulcer patients and their respective controls, between gastric ulcer and duodenal ulcer patients or between acute and convalescent sera in either gastric or duo ... | 1990 | 1966589 |
| prophylactic oral acyclovir in outbreaks of primary herpes simplex virus 1 infection. | | 1990 | 1970099 |
| herpes simplex virus glycoprotein d is recognized as antigen by cd4+ and cd8+ t lymphocytes from infected mice. characterization of t cell clones. | several previous reports have described the surprising inability to detect murine ctl specific for glycoprotein d (gd), one of the important protective immunogens of hsv. using slight variations of published procedures, we were able to show that the immune response to hsv in infected mice includes the generation of ctl specific for gd. c3h/ouj (h-2k) mice were infected by injection in the hind footpads with purified hsv-1. lymphocytes from draining lymph nodes were then isolated and shown to pro ... | 1990 | 1973187 |
| the presence of infectious virus but not conventional antigen can exacerbate graft-versus-host reactions. | previous reports have demonstrated that the introduction of virus (mcmv, hsv-1) concurrent with a graft-versus-host reaction (gvhr) limited to a class i mhc disparity can result in the enhancement of gvhr-associated phenotypic (changes in cd4/cd8 ratio) and functional (inability to produce secondary antibody responses) alterations including the augmentation of in situ natural killer (nk) and donor anti-host cytotoxic t-cell activity. in the present study, we investigated whether immunogens other ... | 1990 | 1975119 |
| detection of viral dna and activation of latent herpes simplex virus in the rabbit neural tissue. | both trigeminal ganglia, brain stem, and cornea from rabbits with established latent herpes simplex virus type 1 (hsv-1) infection were examined by explantation and by spot blot hybridization using strain 17 kpn i fragments i, d, and h and the dna extracted from above mentioned tissues. correlation between positive hybridization and reactivation of infectious virus in the cultured explants was documented by enhanced hybridization with the dna extracts from explanted ganglion samples. in addition ... | 1990 | 1975973 |
| the effect of alpha blockade on iontophoresis-induced ocular shedding of latent hsv-1 w in different host animals. | previous studies demonstrated that non-specific beta blockade promoted ocular shedding of latent hsv-1 in the mouse and rabbit iontophoresis models. the present study examined the effect of topical alpha blockers, thymoxamine and corynanthine, on reactivation and induced ocular shedding of latent hsv-1 w in different host animals. latent trigeminal ganglionic infection was established in balb/c mice and new zealand rabbits following corneal inoculation with hsv-1 w strain, and later confirmed by ... | 1990 | 1980451 |
| herpes simplex virus antigens and inflammatory cells in oral lesions in recurrent erythema multiforme. immunoperoxidase and autoradiographic studies. | herpes simplex virus (hsv) antigens were sought in 15 biopsy specimens from both lesional mucosa and clinically healthy looking oral mucosa between attacks in patients with erythema multiforme (em). four of the eight biopsy specimens obtained from lesional em mucosa stained positively with hsv-1-and/or hsv-2-specific antisera applied in direct immunoperoxidase staining. of the 16 tissue specimens used as controls, two displayed positive staining with hsv-1 and/or hsv-2. five of the seven biopsy ... | 1990 | 1980974 |
| spread of herpes simplex virus (hsv) strains sc16, ang, angpath and its glyc minus and glye minus mutants in dba-2 mice. | herpes simplex virus type 1 (hsv-1) strains sc16, ang, its pathogenic variant angpath and the mutants ang-pathgc18 glycoprotein c (glyc) negative and angpathi2-4 (glye negative) were compared for their ability to spread in dba-2 mice after peripheral inoculation. virus infectivity assay in 9 organs at days 2, 3, 4, 5, 6, and 10 post-infection (p.i.) and morphologic examinations (immunofluorescence, pap staining) showed the following: sc16, ang, and angpath spread first (days 2-3 p.i.) by haemato ... | 1990 | 1981441 |
| latency competence of herpes simplex virus strains ang, angpath and its gc and ge minus mutans. | the latency competence of herpes simplex virus type 1 (hsv-1) strains sc16, kos, ang, angpath and its mutants angpathgc18 (gc minus, spontaneous point mutation), kosgc39 (gc minus deletion), angpathi2-4 (ge minus deletion), and angpathgci-8 (ge and gc minus double mutan) was compared and dba/2 mice. while the latent sc16 and kos reactivated spontaneously in explanted homolateral trigeminal ganglion fragments coming from velaz dba/2 mice, methylation inhibitor 5-azacytidine (5-azac) was required ... | 1990 | 1981458 |
| [52d cologne dermatology meeting of the cologne university dermatology clinic 24 january 1990]. | patients with the following diagnoses were presented: pyoderma gangraenosum in a patient with myelodysplastic syndrome passing into an acute myelomonocytic leukemia and specific cutaneous infiltration, primary genital infection with herpes simplex virus, type 1 (hsv-1) in an adult patient, pellagroid, sweet's syndrome with follicular involvement, sweet's syndrome in a patient with cancer of the breast, lichen amyloidosus, angiolymphoid hyperplasia with eosinophilia, darier's disease 1. associate ... | 1990 | 1982379 |
| role of the major capsid protein in herpes simplex virus type-1 capsid assembly. | two herpes simplex virus type 1 (hsv-1) temperature-sensitive (ts) mutants with defects in the gene for the major capsid protein, icp5, were examined for their effects on virion capsid assembly. polyacrylamide gel electrophoresis revealed that both mutants were able to synthesize wild-type (wt) levels of icp5 at the nonpermissive temperature. however, the 53 kd capsid protein disappeared concomitant with the appearance of a new, 51 kd species. these results, taken together with ultrastructural a ... | 1990 | 1983175 |
| equine herpes virus 1 and pseudorabies virus resistance to 2'-fluoropyrimidine analogs and to bromovinyldeoxyuridine: implications for dtmp kinase activity. | the 2'-fluoropyrimidine nucleoside analogs 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac). 1(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), and 1(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau) showed higher in vitro activity against herpes simplex virus type 1 (hsv-1), than equine herpesvirus 1 (ehv-1) or pseudorabies virus (prv). comparison of the 50% plaque inhibitory doses for hsv-1 and its mutant mmdur-20 in cell cultures with inhibition c ... | 1990 | 1983184 |
| the cytoplasmic tail of hiv-1 gp160 contains regions that associate with cellular membranes. | the hiv-1 envelope glycoprotein gp160 associates with cellular membranes via a discrete transmembrane domain. unlike other retroviral envelope proteins, however, gp160 also forms a secondary association with the lipid bilayer mediated by one or more regions located in the cytoplasmic tail. we have expressed the full cytoplasmic tail sequence of gp160, as a fusion protein with the hsv-1 glycoprotein d signal sequence, transiently in a human embryonic kidney cell line. our results show that in the ... | 1991 | 1984664 |
| analysis of dna sequences present in complexes of v-myc and cellular dna. | we have isolated cellular dna sequences from the myelocytomatosis virus-transformed quail fibroblastic cell-line (mc29-q8) by indirect immunoprecipitation of v-myc-dna complexes and subsequent cloning of the dna. the v-myc-dna complexes were obtained from isolated nuclei pretreated with 150 mm salt and indirect immunoprecipitation of the p110 gag-myc protein with the igg of a gag-specific monoclonal antibody. a non-specific monoclonal igg was used as control to account for non-specific interacti ... | 1991 | 1992445 |
| colonization of adrenal glands and ovaries of mice by variants of hsv 1 and 2. ii. histopathological, immunohistochemical and in situ hybridization studies. | the herpes simplex virus (hsv)-infected mouse model was used to correlate histopathological lesions in adrenal glands and ovaries with the localisation of viral nucleic acids and viral antigens, employing in situ hybridization and immunohistochemistry. in the adrenals, the lesions were mainly restricted to the zona fasciculata and the zona reticularis, sometimes extending to the medulla. in the ovaries, lesions were detected in follicles and in the stroma. during the course of infection, hsv nuc ... | 1991 | 2018430 |
| ischaemic myelopathy secondary to disseminated intravascular coagulation in aids. | a 39-year-old patient with aids presented with a rapidly progressive myelopathy with a partial brown-séquard syndrome. he died, 9 weeks after onset of the first neurological signs, from diffuse encephalopathy. neuropathological examination revealed multiple, usually small, frequently haemorrhagic, infarcts or various ages and numerous fibrin thrombi in medium and small penetrating vessels and capillaries of the brain and spinal cord, characteristic of disseminated intravascular coagulation. ther ... | 1991 | 2030375 |
| variables predicting oral mucosal lesions in allogenic bone marrow recipients. | one hundred and forty-eight (72%) of 205 allogenic bone marrow transplant recipients developed mucosal lesions in the oral cavity during the aplastic period after transplantation. lesions were most frequent 8 to 9 days after transplantation. factors that correlated with the development of mucosal lesions after univariate and multivariate analysis were a marrow cell dose of less than 3 x 10(8) cells/kg body weight, (p less than 0.0001), prolonged aplastic period (white blood cell count less than ... | 1991 | 2037474 |
| early loss of astrocytes in herpes simplex virus-induced central nervous system demyelination. | immunohistochemistry was used to study herpes simplex virus type 1-induced central nervous system demyelination in the trigeminal root entry zone of mice inoculated with herpes simplex virus type 1 by the corneal route. there was no change in peripheral nervous system myelin as shown by immunostaining for p0 glycoprotein. double immunoperoxidase staining for herpes simplex virus type 1 antigens and glial fibrillary acidic protein showed that most of the infected cells were astrocytes. glial fibr ... | 1991 | 2042945 |
| [amoebic keratitis caused by acanthamoeba sp. may hsv1 infection play a role?]. | many cases of human keratitis with acanthamoeba sp. have a pseudo-herpetic appearance. the authors therefore evaluated the importance of co-infection with this virus in the onset of amoebic cornean lesions. after herpetic and amoebic co-infection rabbits showed severe cornean lesions, the viral aspect of cornea received a specific treatment. the amoebic co-infection progressed unchecked with severe lesions until day 37 p.i., in which trophozoites and cysts were numerous. the possible favourising ... | 1991 | 2050960 |
| synthesis and antiviral activity of 5-heteroaryl-substituted 2'-deoxyuridines. | the synthesis of 5-heteroaryl-substituted 2'-deoxyuridines is described. the heteroaromatics were obtained from three different 5-substituted 2'-deoxyuridines. cycloaddition reaction of nitrile oxides on the 5-ethynyl derivative 1 gave the isoxazoles 4a-e. the thiazole derivatives 14a-c were obtained from the 5-thiocarboxamide 11, while 5-pyrrol-1-yl-2'-deoxyuridine (17) could be synthesized directly from 5-amino-2'-deoxyuridine. the compounds were evaluated for antiviral activity. selective act ... | 1991 | 2061920 |
| nucleic acid related compounds. 65. new syntheses of 1-(beta-d-arabinofuranosyl)-5(e)-(2-iodovinyl)uracil (ivarau) from vinylsilane precursors. radioiodine uptake as a marker for thymidine kinase positive herpes viral infections. | (trimethylsilyl)acetylene was coupled with 1-(2,3,5-tri-o-acetyl-beta-d- arabinofuranosyl)-5-iodouracil to give 1- (2,3,5-tri-o-acetyl-beta-d-arabinofuranosyl)-5-[2-(trimethylsilyl)eth yny l] uracil. lindlar hydrogenation of 4 gave 1-(2,3,4-tri-o-acetyl-beta-d-arabinofuranosyl)-5(z)-[2- (trimethylsilyl)vinyl]uracil. treatment of 5 with iodine monochloride (or sodium iodide/phenyliodine(iii) dichloride) in benzene gave 1-(2,3,5-tri-o-acetyl-beta-d-arabinofuranosyl)-5(e)-(2-iodovinyl)uracil (7), w ... | 1991 | 2067000 |
| efficacy of (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine in various models of herpes simplex virus infection in mice. | the phosphonylmethoxyalkyl derivative (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc) was evaluated for its in vivo efficacy in several model infections for herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and thymidine kinase-deficient (tk-) hsv-1 in mice. in hairless mice infected intracutaneously with hsv-1 or hsv-2, hpmpc completely suppressed all manifestations of the disease (skin lesions, paralysis of the hind legs, and mortality) if it was administered topically at a co ... | 1991 | 2069375 |
| effect of a thymidine kinase inhibitor (l-653,180) on antiviral treatment of experimental herpes simplex virus infection in mice. | thymidine kinase (tk) inhibitors can block the activity of tk-dependent antiviral drugs in vitro. we have examined the ability of the tk inhibitor (+/-)-9-([(z)-2-(hydroxymethyl)cyclohexyl] methyl)guanine (l-653,180) to prevent the therapeutic effect of acyclovir (acv) in experimental herpes simplex virus type 1 (hsv) skin infections of mice. the results showed that acv given in the drinking water prevents, in a dose-dependent way, the evolution of the viral infection, and that l-653,180 can rev ... | 1990 | 2088206 |
| oral herpetic lesions in an edentulous patient. | this article reports a case of oral herpetic lesions that recurred several times over 1 1/2 years in an edentulous 65-year-old woman. the patient was under treatment for complete dentures at the new jersey dental school. a differential diagnosis of mucosal ulcerative lesions is described, along with the laboratory methodology for confirmation of herpes simplex virus type 1 (hsv-1) infection. | 1990 | 2097054 |
| fibronectin involvement following herpes simplex infection of cultured rat astrocytes. | to evaluate the potential impact of a strongly neurotropic virus on the matricial fibronectin (fn) of a given neural cell type, first subculture of rat astrocytes were inoculated with either a low (0.1-0.5) or a high moi (10-100) of herpes simplex virus-type 1 (hsv-1). infected samples, as well as matched controls, were harvested from 12 to 72 h post-inoculation and processed for extracellular fn labeling by the peroxidase-antiperoxidase (pap) method. following low moi, multinucleated cell forma ... | 1990 | 2097577 |
| [hiv-associated periodontal lesions]. | during hiv infection different lesions may occur in the area of the gingiva and/or the periodontium. an increased frequency and severity of periodontal diseases has been observed. different forms of candida albicans infection have been clinically characterized as pseudomembranous, erythematous (atrophic) or hyperplastic form or as papillary variant. while infection with candida albicans may occur frequently in other areas of the oral mucosa, candidiasis of the gingiva seems to be quite rare. due ... | 1990 | 2100235 |
| vectorial apical delivery and slow endocytosis of a glycolipid-anchored fusion protein in transfected mdck cells. | to characterize the mechanisms that determine the apical polarity of proteins anchored by glycosylphosphatidylinositol (gpi), we studied the targeting of a gpi-anchored form of a herpes simplex glycoprotein, gd-1, in transfected mdck cells. using a biotin-based targeting assay, we found that gpi-anchored gd-1 was sorted intracellularly and delivered directly to the apical surface. endocytosis of gpi-anchored gd-1 occurred slowly and preferentially from the apical domain, while transcytosis of th ... | 1990 | 2145577 |
| recombinogenic properties of herpes simplex virus type 1 dna sequences resident in simian virus 40 minichromosomes. | in a previous work, it was demonstrated that the bacterial transposon tn5 is capable of undergoing sequence inversion via recombination between its duplicated is50 elements when replicated by the herpes simplex virus type 1 (hsv-1) origin oris but not by the simian virus 40 (sv40) origin orisv. further analysis of the latter phenomenon indicated that this lack of recombination was the result of topological constraints imposed by the sv40 minichromosome, such that recombination events could be re ... | 1990 | 2152818 |
| protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein b constitutively expressed in human cells with a bk virus episomal vector. | the herpes simplex virus type 1 (hsv-1) glycoprotein b (gb-1) gene, deleted of 639 nucleotides that encode the transmembrane anchor sequence and reconstructed with the extramembrane and intracytoplasmic domains, was cloned under control of the rous sarcoma virus long terminal repeat in the episomal replicating vector prp-rsv, which contains the origin of replication and early region of the human papovavirus bk as well as a cdna for a mutant mouse dihydrofolate reductase that is resistant to meth ... | 1990 | 2152829 |
| expression of the herpes simplex virus type 1 glycoprotein c gene requires sequences in the 5' noncoding region of the gene. | the role of the 5' noncoding region of the herpes simplex virus type 1 glycoprotein c (gc) gene in viral gene expression was investigated with recombinant herpesviruses that contained the bacterial beta-galactosidase gene under the control of the gc promoter-regulatory region. each of these viruses had the same dna sequences from the start of gc transcription upstream to -114 but had variable segments of the downstream 140-base-pair sequence that is between the start of gc transcription and tran ... | 1990 | 2152831 |
| release of rna polymerase from vero cell mitochondria after herpes simplex virus type 1 infection. | infection of vero cells with herpes simplex virus type 1 results in the appearance in soluble extracts of a dna primase activity. the partially purified enzyme, mr, approximately 100,000, is identical in resistance to alpha-amanitin, ph profile, mg2+ dependence, salt sensitivity, and kmatp to the catalytic core of vero cell mitochondrial rna polymerase. moreover, the products synthesized are those expected of an rna polymerase rather than a dna primase. inasmuch as the enzyme is not present in s ... | 1990 | 2152832 |
| characterization of dna-protein complex formation in nuclear extracts with a sequence from the herpes simplex virus thymidine kinase gene. | the biochemical characteristics of complex formation in nuclear extracts from mock-infected and herpes simplex virus (hsv)-infected vero and hela cells with a sequence downstream of and adjacent to the promoter for the hsv thymidine kinase gene were studied using the mobility shift electrophoresis assay. this region is bound by host cell proteins, as evidenced by the formation of complexes after incubation in extracts from mock-infected cells. unique virus-specific complexes form in extracts pre ... | 1990 | 2153138 |
| fluoro carbocyclic nucleosides: synthesis and antiviral activity of 2'- and 6'-fluoro carbocyclic pyrimidine nucleosides including carbocyclic 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil and carbocyclic 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil. | the racemic carbocyclic 2'-fluoroarabinosyl pyrimidine nucleosides 8, 9 (c-fiau), 12, and 13 (c-fmau) and the 2'-fluororibosyl pyrimidine nucleosides 17, 20, and 21 were prepared from their respective protected 2'-fluoro amino diols 5 and 14. the carbocyclic 2'-2'-difluorothymidine analogue 27 was obtained from the protected difluoro amino diol 24 which was prepared from the ketone 23 and (diethylamino)sulfur trifluoride (dast). the chiral carbocyclic 2'-deoxy-6'-fluorouridines 33, 34, 38, and 3 ... | 1990 | 2153201 |
| influence of peptide acylation, liposome incorporation, and synthetic immunomodulators on the immunogenicity of a 1-23 peptide of glycoprotein d of herpes simplex virus: implications for subunit vaccines. | a peptide corresponding to residues 1 to 23 of glycoprotein d of herpes simplex virus type 1 was chemically synthesized and coupled to a fatty acid carrier by standard merrifield synthesis procedures. the resulting peptide-palmitic acid conjugate (acylpeptide) exhibited enhanced immunogenicity in mice as compared with that exhibited by the free form of the peptide. incorporation of the acylpeptide into liposomes further increased the immunogenicity of the peptide, while inclusion of the immunomo ... | 1990 | 2153234 |
| cystatin c, a human proteinase inhibitor, blocks replication of herpes simplex virus. | cystatin c is a human cysteine proteinase inhibitor present in extracellular fluids. cystatin c and a tripeptide derivative (z-lvg-chn2) that mimics its proteinase-binding center, were tested for possible antiviral activity against herpes simplex virus type 1 (hsv) and poliovirus type 1. both recombinant cystatin c and z-lvg-chn2 displayed strong inhibitory effects on hsv replication, whereas no significant effect on poliovirus replication was seen. the molar concentration of cystatin c that gav ... | 1990 | 2153254 |
| comparative laboratory diagnosis of experimental herpes simplex keratitis. | we compared two commercially available tests, a direct immunofluorescence assay and an enzyme-linked immunosorbent assay (elisa), to viral isolation in tissue culture for the laboratory diagnosis of untreated and partially treated experimental herpes simplex virus keratitis. new zealand albino rabbits were inoculated bilaterally with herpes simplex virus-1 mckrae strain after corneal scarification. one eye of each rabbit was treated with a 1% trifluorothymidine solution daily, starting on the th ... | 1990 | 2153342 |
| evidence for an involvement of thymidine kinase in the excision repair of ultraviolet-irradiated herpes simplex virus in human cells. | a wild-type strain of herpes simplex virus type 1 (hsv-1:kos) encoding a functional thymidine kinase (tk+) and a tk- mutant strain (hsv-1:ptk3b) were used to study the role of the viral tk in the repair of uv-irradiated hsv-1 in human cells. uv survival of hsv-1:ptk3b was substantially reduced compared with that of hsv-1:kos when infecting normal human cells. in contrast, the uv survival of hsv-1:ptk3b was similar to that of hsv-1:kos when infecting excision repair-deficient cells from a xeroder ... | 1990 | 2153540 |
| viral lower respiratory tract infections in filipino children. | viral causes of acute lower respiratory tract infection were studied prospectively between 15 june and 31 october 1984 in 312 filipino children less than 5 years old living in periurban slums and middle-class housing. the cause was based on viral antigen detection, virus isolation, and antibody assays. there were 131 children (41.2%) who were admitted to the hospital, and 150 (47%) had an infiltrate on chest radiograph. a total of 198 viral infections were confirmed in 162 patients (51.9%), 42.3 ... | 1990 | 2153734 |
| herpes simplex virus type 1 infection of rat astrocytes in primary culture: effects of dibutyryl cyclic amp. | monolayer cultures of primary rat astrocytes grown with or without dibutyryl cyclic amp (dbcamp) for two weeks or longer were infected with round plaque-forming (rd) or syncytia-forming (syn) variants of herpes simplex virus type 1 (hsv-1). infection with hsv-1 did not stimulate synthesis of glial fibrillary acidic protein (gfap) or alter the general organization of the intermediate (glial) filaments in astrocyte cultures. however, the dbcamp-treated astrocytes produced 10- to 100-fold lower tit ... | 1990 | 2153759 |
| infection of cultured central nervous system neurons with a defective herpes simplex virus 1 vector results in stable expression of escherichia coli beta-galactosidase. | we have developed a defective herpes simplex virus (hsv) vector system that permits the introduction of virtually any gene into mammalian central nervous system neurons. the prototype vector, phsvlac, contains a transcription unit that places the escherichia coli lacz gene under the control of the hsv-1 immediate early 4/5 promoter. phsvlac was propagated using the hsv-1 temperature-sensitive mutant ts k as helper virus. infection of rat neurons in primary culture derived from various regions th ... | 1990 | 2153970 |
| mapping of low abundance latency-associated rna in the trigeminal ganglia of mice latently infected with herpes simplex virus type 1. | during herpes simplex virus type 1 (hsv-1) latent infection of the mouse trigeminal ganglion there is limited viral gene expression. the latency-associated transcripts (lat) map approximately to the psti-mlui fragment within the bamhi b and bamhi e fragments (long repeat regions) of the viral genome. additional weak hybridization signals have been detected by in situ hybridization that correspond to transcription from hsv-1 dna fragments adjacent tot he psti-mlui fragment. we mapped the region e ... | 1990 | 2154530 |
| antibody response to herpes simplex virus glycoproteins gb and gd. | the antibody response to herpes simplex virus (hsv) glycoproteins b and d was evaluated using these cloned glycoproteins in an elisa assay and compared to a standard western blotting procedure. the elisa assay appeared to be more sensitive for detecting gb and gd antibodies. antibodies to gb but not gd were detected in the acute sera of patients presenting with their first episode of true primary genital herpes. the geometric mean hsv gb titer (log 2) was also significantly higher than the geome ... | 1990 | 2154542 |
| herpes simplex virus 1 infection upregulates stress protein expression in cultured retinal neurons. | the production of a 57k stress protein (strp) after herpes simplex virus type 1 (hsv-1) infection was examined in cultured neonatal rat retinal cells. strp expression in individual cells was identified using a monoclonal antibody, ti56. indirect immunofluorescence of uninfected retinal cultures showed that approximately 40% of cells expressed neurofilament (nf+) and 5% expressed a low level of strp. following hsv infection the proportion of nf+ cells decreased while the proportion of strp positi ... | 1990 | 2154558 |
| the herpes simplex virus 1 gene for icp34.5, which maps in inverted repeats, is conserved in several limited-passage isolates but not in strain 17syn+. | in a previous study, it was reported that herpes simplex virus 1 (hsv-1) strain f contains a transcribed open reading frame situated in the inverted repeats of the l component between the terminal a sequence and the open reading frame that encodes the alpha 0 gene (j. chou and b. roizman, j. virol. 57: 629-637, 1986). by means of an antibody to repeats of the trimer ala-thr-pro predicted to be specified by the open reading frame, it was shown that the open reading frame specifies a protein (m. a ... | 1990 | 2154589 |
| identification and characterization of the herpes simplex virus type 2 gene encoding the essential capsid protein icp32/vp19c. | we describe the characterization of the herpes simplex virus type 2 (hsv-2) gene encoding infected cell protein 32 (icp32) and virion protein 19c (vp19c). we also demonstrate that the hsv-1 ul38/orf.553 open reading frame (orf), which has been shown to specify a viral protein essential for capsid formation (b. pertuiset, m. boccara, j. cebrian, n. berthelot, s. chousterman, f. puvian-dutilleul, j. sisman, and p. sheldrick, j. virol. 63: 2169-2179, 1989), must encode the cognate hsv type 1 (hsv-1 ... | 1990 | 2154597 |
| localization on the herpes simplex virus type 1 genome of a region encoding proteins involved in adsorption to the cellular receptor. | we have previously shown that aminoglycosides such as neomycin and the polyamino acids polylysine and polyarginine selectively inhibit the binding of herpes simplex virus type 1 (hsv-1) to the cellular receptor, whereas hsv-2 infection is unaffected. in the present study we took advantage of this difference between hsv-1 and hsv-2 by using hsv(-1)-hsv(-2) intertypic recombinants to locate a region on the hsv-1 genome encoding proteins affecting the binding of the virion to the cellular receptor. ... | 1990 | 2154609 |