| isolation and characterization of ca2+-dependent modulator protein from the marine invertebrate renilla reniformis. | an acidic, low molecular weight (18 400--19 100) protein capable of activating porcine brain phosphodiesterase in the presence of calcium has been purified 2700-fold from the anthozoan coelenterate, renilla reniformis. the protein has physical, spectral, and chemical properties similar to those of modulator proteins isolated from mammalian species. amino acid composition studies reveal no significant differences between the renilla and mammalian modulator proteins. for example, we observed 1 mol ... | 1979 | 33694 |
| enzymatic resynthesis of the hydrolyzed peptide bond(s) in ribonuclease s. | | 1979 | 33699 |
| application of the ph-jump method to the titration of tyrosine residues in bovine alpha-lactalbumin. | a stopped-flow technique has been developed for the zero-time spectrophotometric titration of tyrosine residues in the purely native or in the purely alkaline denatured state of alpha-lactalbumin that undergoes an alkaline conformational transition in the ph region of tyrosine ionization. the progressive absorption change at 298 nm caused by a ph jump from neutral ph is shown to result from the change in ionization of the tyrosine residues brought about by a first-order process of the conformati ... | 1979 | 33704 |
| glycosylation of kappa-casein. i. localization and characterization of sialyltransferase in bovine mammary gland. | a sialyltransferase (cmp-n-acetylneuraminate:d-galactosyl-glycoprotein n-acetylneuraminyltransferase, ec 2.4.99.1) which attaches n-acetylneuraminic acid to the terminal end of the carbohydrate chain of kappa-casein was found to be concentrated in golgi apparatus-enriched fractions of bovine mammary gland. maximum sialyltransferase activity was obtained at ph 5.5 and 37 degrees c in the presence of 1 mm dithiothreitol and triton x-100. a km of 0.19 mg asialo-kappa-casein/ml (0.01 mm) was obtaine ... | 1979 | 33713 |
| calcium-dependent cyclic nucleotide phosphodiesterase. inhibition of basal activity by heat-stable factors from rat cerebrum. | the boiled supernatant fraction from rat cerebrum contained factors which inhibited the basal activity of a ca2+-dependent phosphodiesterase from rat cerebrum. two inhibitory fractions were isolated by deae-cellulose or sephadex chromatography and were deemed proteins, based on their sensitivity to trypsin digestion. the inhibitory fractions eluted from deae-cellulose columns prior to the ca2+-dependent activator protein. the inhibitory factors, unlike the activator protein, were stable to heat ... | 1979 | 33721 |
| [hereditary cleft lips (cheilognathoschisis) combined with cryptorchism in holstein-friesian cattle (author's transl)]. | | 1979 | 33790 |
| use of ca--egta buffers for transposing ca2+ efflux into ph changes. application to rod outer segment membrane vesicles. | | 1979 | 33833 |
| proceedings of the international embryo transfer society. fifth annual conference - 14-16 january, 1979. | | 1979 | 33864 |
| repulsive van der waals forces. i. complete dissociation of antigen-antibody complexes by means of negative van der waals forces. | | 1979 | 33892 |
| identification and observation of alkyl proton resonances of the amino-terminal residues of bovine neurophysins. evidence for conformational differences between neurophysin-i and neurophysin-ii. | analysis of the 220 mhz proton magnetic resonance spectra of bovine neurophysins-i and -ii and of the effects of ph and succinylation of these spectra has allowed identification of the -ch3 proton resonances of the amino-terminal alanine of both proteins and of the -ch3 resonances of methionine-2 of neurophysin-ii. the alanine -ch3 resonance of neurophysin-i is a sharp doublet at all ph values between 1 and 10.5 indicating relatively few restrictions on its mobility. by contrast, the -ch3 resona ... | 1979 | 33930 |
| studies of the interaction of bovine neurophysin-ii with [1-hemi-d-(3-13c)cystine] oxytocin and [1-hemi-(3-13c)cystine] oxytocin. | | 1979 | 33931 |
| ph dependency of kinetic parameters and reaction mechanism of beef liver catalase. | the ph-dependence of the kinetic parameters in h2o2 decomposition by beef liver catalase was investigated. at ph 7.0, the ternary complex (ess) decomposition rate was about 100 times faster than ess formation (42 microm h2o2), and the value of the michaelis constant was 0.025 m. from ethanol competition experiments, two different proton dissociation constants of the enzyme (pke1 = 5.0, pkes2 = 5.9) were obtained for the binding of first and second h2o2 molecules. another pka value (pkes1) of 4.2 ... | 1979 | 33977 |
| selective extraction of marker enzymes of bovine milk fat globule membrane by nonionic detergents. | large amounts (66-97%) of marker enzymes such as alkaline phosphatase, 5'-nucleotidase, phosphodiesterase i, and gamma-glutamyl transpeptidase of bovine milk fat globule membrane (mfgm) were selectively solubilized by nonionic detergents such as triton x-100, tween 20, nonidet p-40, liponox nck, and emulgen 109-p. on the other hand, the extractability of mfgm protein with these detergents was less than 50%. judging from the recovery of total activity, it is likely that alkaline phosphatase, phos ... | 1979 | 33979 |
| cytochrome c1 complexes. | cytochrome c1 forms an active complex with cytochrome c as previously reported (chiang, y. l., kaminsky, l. s., and king, t. e. (1976) j. biol. chem. 251, 29-36). it also forms a complex with cytochrome oxidase with heme ratio of 1:1. this cytochrome c1.oxidase complex has been purified by ammonium sulfate fractionation and is stable in media of high ionic strength (greater than 0.1 m) but dissociates as the ph deviates from neutral. the purified cytochrome c1 aggregates to an oligomer, presumab ... | 1979 | 33986 |
| the effects of bovine albumin on the vascular permeability of the perfused rat mesentery [proceedings]. | | 1978 | 34028 |
| adenosine triphosphate in the bovine chromaffin granule. | 1. ph and potential gradients are generated across the membranes of chromaffin granule 'ghost' by incubating them with mgatp: the inside of the 'ghosts' is positive and acid with respect to the incubation medium. 2. the ph gradient is partially dissipated by inclusion of a substrate for the catecholamine pump, or a mitochondrial uncoupling agent, but is enhanced by reserpine. 3. an imposed ph gradient leads to amine uptake by the 'ghosts': a potential gradient leads to atp uptake. studies with i ... | 1978 | 34031 |
| the maintenance of motility and the surface properties of epididymal spermatozoa from bull, rabbit and ram in homologous seminal and epididymal plasma. | epididymal spermatozoa from bull, rabbit and ram were incubated in homologous epididymal plasma or seminal plasma in a buffered saline-based medium with or without serum albumin. the spermatozoa were either diluted directly into the medium or were washed first. no effect of washing was observed on the subsequent reaction of the cells to the different media. a considerable proportion of the populations of epididymal spermatozoa survived (i.e. continued to exhibit motility) for up to 22 h at 30 de ... | 1979 | 34036 |
| control of glyceraldehyde-3-phosphate dehydrogenase in cardiac muscle. | | 1979 | 34041 |
| ph, titratable acidity & osmolality of human breast milk & some infant milk formulae. | | 1978 | 34062 |
| study of the two pathways for arachidonate oxygenation in blood platelets. | during collagen-induced blood platelet aggregation, arachidonic acid is set free from membrane phospholipids and subsequently converted into 12-hydroxyeicosatetraenoic acid by arachidonate lipoxygenase and into thromboxane a2, 12-hydroxyheptadecatrienoic acid (hete) and malondialdehyde by cyclooxygenase and thromboxane synthase. lipoxygenase and cyclooxygenase have optimal activity at neutral to basic ph, while the thromboxane synthase is ph-independent between 5 and 9. these enzymes are membran ... | 1979 | 34075 |
| [methylviologen photoreduction by chloroplasts]. | the condition of methylviologen photoreduction by chloroplasts was investigated. argon bubbling through the suspension of chloroplasts or degasing in vacuum caused inhibition of methylviologen reduction probably due to the denaturation of chloroplast membranes at the water/air boundary. adding glycerol or bovine serum albumine or removing oxygen from chloroplast suspension with the aid of the oxygen absorbing-systems preserved the activity of chloroplasts. methylviologen photoreduction is inhibi ... | 1978 | 34095 |
| mechanochemical properties of brain clathrin: interactions with actin and alpha-actinin and polymerization into basketlike structures or filaments. | two molar urea (ph 7.5) and column chromatography on sepharose 4b were used to separate clathrin (coat protein) from the membrane of coated vesicles from bovine brain. lytron (polystyrene) particles were used for study of the interaction of clathrin with contractile proteins. muscle g-actin, f-actin, and alpha-actinin were bound by clathrin-coated lytron particles, while no interaction was found when muscle tropomyosin and serum albumin were tested. clathrin molecules dispersed in a solution of ... | 1979 | 34147 |
| digestion, absorption and transport of lipids in ruminant animals. | | 1978 | 34168 |
| [genetically conditioned variability of metabolic profile parameters in dairy cows]. | individual heritability and differences in the concentration of the chemical components of the blood were studied in the dairy cows of the slovak spotted breed. the experiment was performed with 166 cows. the set comprised six groups of half-sisters from three stocks. the differences among the cows were statistically significant (alpha = 0.01) in the majority of the parameters studied: haematocrit, haemoglobin, ph, po2, oxygen saturation of the blood; plasma potassium, phosphorus, magnesium, cal ... | 1978 | 34253 |
| [evaluation and comparison of acid-base findings obtained using the partial and astrup's technic in cattle]. | the aim of the study was to compare the acid-base (ab-) findings in cattle obtained parallelly by the equilibration method after astrup and the partial method (titration determination of total carbon dioxide in blood plasma) and at the same time to determine ph, net acid-base exudate in urine and the specific weight of urine. altogether we made 65 parallel examinations in three groups of cows according to phases of reproduction. in 70.8% of cases correlation between the two results was very good ... | 1979 | 34255 |
| characteristics of gastric secretion in suckling calves. | in the investigations on five calves with fistulae in the abomasum and oesophagus the secretion of gastric juice as well as its content of proteolytic enzymes and hydrogen ions were measured. it was found that the gastric juice of calves possessed a fairly high proteolytic activity (about 6 up/l) and hydrogen ion level already on the 3rd day of life. sham feeding produced two different types of gastric secretion response, depending on the age of calves. in the first week of life sham feeding cau ... | 1978 | 34313 |
| presence of a lipolytic factor in the gastric juice of calves. | in experiments performed on 5 calves the lipolytic activity of pure gastric juice obtained from the abomassum was investigated during the first 2 weeks of life. it was found that the gastric juice of suckling calves possesses the ability of releasing free fatty acids from all triglycerides present in the milk. the highest activity was observed, however, using monoglyceride--tween 80 as a substrate. the optimum ph for the lipolytic reaction approached 3.0. | 1978 | 34314 |
| minoxidil in refractory hypertension. effects on blood pressure, plasma volume and muscle blood flow. | | 1979 | 34316 |
| the role of the colostrum in relation to immunity and survival in the newborn ruminant and pig. a seminar held in the institut national agronomique de paris-grignon, 78850 thiverval-grignon, france. april 25, 26 and 27th 1978. | | 1978 | 34350 |
| milk ph, acid base status, and growth in babies. | metabolic acidosis is common in babies fed cows' milk-based formulae. therefore the effects of adding alkaline salts (sodium and potassium citrate) to a demineralised whey formula were studied in vitro and in 26 low birthweight babies fed on the formula or formula plus citrate. the alkali altered the ph and titratable acidity to a value nearer human milk but it increased the buffering capacity to a value further away. this may effect the bacterial flora of the intestine. the babies fed on formul ... | 1978 | 34363 |
| purification and properties of four species of lysyl oxidase from bovine aorta. | lysyl oxidase of bovine aorta was resolved into four enzymically active species by elution from deae-cellulose with a salt gradient in 6m-urea, consistent with purification results obtained with enzyme of other tissues [stassen (1976) biochim. biophys. acta438, 49-60]. in the present study, each of the four peaks of activity was purified to apparent homogeneity by subsequent chromatography on gel-filtration media in 6m-urea. each enzyme is eluted as a species with mol.wt. approx. 30000 under the ... | 1979 | 34386 |
| interaction of dinitrophenyl groups bound to bovine serum albumin with univalent fragments of anti-dinitrophenyl antibody. | two lysine residues of bovine serum albumin reacted with 1-fluoro-2,4-dinitrobenzene with apparent second-order rate constants approx. 500-times greater than those observed in similar reactions with low-molecular-weight lysine derivatives. a series of dinitrophenyl (dnp)-bovine serum albumins were prepared and their ability to bind univalent fragments of anti-dnp antibody was measured by fluorescence-quenching titrations. compared with the dnp group of the free hapten, 6-n-dnp-aminohexanoate, th ... | 1979 | 34387 |
| properties of the cupric sites in bovine superoxide dismutase studied by nuclear-magnetic-relaxation measurements. | proton nuclear-relaxation rates have been measured as a function of frequency, temperature, ph and cyanide concentration in aqueous solutions of superoxide dismutase from bovine erythrocytes. the results show that, whereas for ph less than or equal to 9 only one water molecule is bound to each cu2+ ion, at higher ph a second co-ordination site for oh- becomes available; it is proposed that this involves cleavage of the bond between cu2+ and the histidine residue that bridges to zn2+. | 1979 | 34390 |
| ph dependence of magnesium ion binding to prothrombin fragment 1 and gamma-carboxyglutamic acid-containing peptides via 25mg nmr. | | 1979 | 34393 |
| tyrosine hydroxylase:--examination of conditions influencing activity in pheochromocytoma, adrenal medulla and striatum. | | 1979 | 34406 |
| fluorescence studies of native and modified neurophysins. effects of peptides and ph. | the effect of neurophysin-hormone interaction on the environment of the single tyrosine of bovine neurophysin (tyr-49) and on that of the tyrosine of oxytocin and vasopressin was studied by fluorescence; tyrosine-free peptides were used to determine effects on tyr-49, and acetylated neurophysin was used to determine effects on the hormone tyrosine. binding increases the fluorescence intensity of tyr-49 by 130% while the fluorescence of the hormone tyrosine is almost completely quenched. correlat ... | 1979 | 34422 |
| isolation and partial characterization of intermediate filament protein (skeletin) from cow heart purkinje fibres. | the intermediate filament protein skeletin from cow heart purkinje fibres was purified to homogeneity by a selective extraction procedure and gel chromatography in the presence of sodium dodecyl sulphate. monospecific antibodies were obtained by immunisation of rabbits with the sodium dodecyl sulphate-skeletin complex, and rocket electrophoresis made it possible to quantify the concentration of protein. the skeletin monomer has a molecular weight of 55 000. amino acid analysis revealed that skel ... | 1979 | 34442 |
| in vitro phosphorylation of a purified preparation of bovine corpus striatal tyrosine hydroxylase. | | 1978 | 34498 |
| in vitro degradation of enkephalin: evidence for cleavage at the gly-phe bond. | | 1978 | 34499 |
| thyrotropin binding to cultured lymphocytes and thyroid cells. | the tsh-binding properties of human lymphocytes in continuous culture were studied and compared to those of bovine and human thyroid cells in primary culture. both lymphocytes and thyroid cells had maximal tsh-binding capacity at ph 5.2. at ph 7.4, thyroid cells bound 15% but lymphocytes bound only 3% of the amount bound at ph 5.2. at 37 c, maximal binding of [125i]iodo-tsh to lymphocytes was reached within 60--90 min and maximal binding to thyroid cells was reached within 15--20 min. tsh bindin ... | 1978 | 34508 |
| binding of rat and bovine thyrotropin to specific sites in rat thyroid tissue. | | 1978 | 34509 |
| investigations on the activation of bovine prochymosin. | activation of prochymosin at ph below 2.5 results in formation of the active enzyme pseudochymosin by proteolytic cleavage of the bond 27--28. pseudochymosin is 15 amino acid residues longer than chymosin. it is the final activation product at low ph, whereas chymosin is formed by activation between ph 4 and 5. pseudochymosin is converted to chymosin when it is brought to ph 5.5. our present knowledge does not allow quantitative evaluation of the possible reactions involved in formation of pseud ... | 1979 | 34519 |
| alkaline phosphatase from bovine mammary tissue: purification and some molecular and catalytic properties. | | 1979 | 34540 |
| molecular forms of alkaline phosphatase in bull seminal plasma--i. isolation and characterization of two forms. | | 1979 | 34541 |
| transglutaminase-catalysed labelling of cytochrome c and cytochrome c oxidase [proceedings]. | | 1979 | 34544 |
| adrenodoxin reductase.adrenodoxin complex. flavin to iron-sulfur electron transfer as the rate-limiting step in the nadph-cytochrome c reductase reaction. | | 1979 | 34608 |
| some effects of the administration of endotoxin in mice. specific cleavage of serum albumin by an acid protease and the generation of amyloid serum component. | endotoxin has been shown to induce amyloidosis in mice and to result in the appearance in serum of large amounts of amyloidrelated protein (saa). after injection of 300 mug lipopolysaccharide escherichia coli, saa behaves as an acute phase reactant with levels reaching a peak of >600 mug/ml at 18-22 h and returning to base line (<50 mug/ml) by 48 h in each of four strains tested; only the endotoxin-resistant c3h/hej strain showed a smaller response. lesser, though significant, elevations were al ... | 1979 | 34628 |
| use of the butaclamol template in a search for antipsychotic agents with lessened side effects. | a number of molecular similarities between the antipsychotic agents butaclamol and clozapine were noted. based on the premise that this was a strong indicator of a common mechanism of action (i.e., binding at the antagonist state of the dopamine receptor), a research approach was described. three simplified analogues (4,8, and 12a) of butaclamol which still retained the molecular functionalities of the parent structure were synthesized and tested in the haloperidol receptor assay. 1-(5-methyl-10 ... | 1979 | 34721 |
| concurrent daily infection of calves with fasciola hepatica and ostertagia ostertagi. | three groups of calves were infected daily with either 1500 ostertagia ostertagi larvae, 20 fasciola hepatica metacercariae, or 1500 o ostertagi plus 20 f hepatica metacercariae. weekly measurements were taken of calf weight, faecal egg output, plasma concentrations of albumin, plasma activities of sorbitol dehydrogenase, gamma glutamyl transpeptidase and pepsinogen and standard haematological indices. calves were killed either 10 or 21 weeks after daily infections began. f hepatica infection ha ... | 1978 | 34850 |
| some biochemical parameters for qualification of bull semen. | since the macroscopical and microscopical examination of bull semen does not in all cases appear to give conclusive indications concerning the fertilizing capacity, an investigation of some biochemical parameters was undertaken. it was shown that the following biochemical examination could furnish some more information about the quality of the semen: (1) determination of the phenylalanine- alpha-ketoglutarate transminase activity both in the seminal plasma and in the whole semen. a high transmin ... | 1979 | 34902 |
| bruising and muscle ph with mixing groups of cattle pre-transport. | the effects on bruising and muscle ph of mixing cows and steers at loading, and 96 hours before loading, for transport to slaughter were examined. a second experiment investigated the effect on bruising of mixing two strange groups of steers at loading, 24 and 96 hours before loading. all cattle were hornless bos indicus crossbreds from the same property in north queensland. the bruise score, bruise trim weight and muscle ph of mixed cows and steers did not differ significantly from that of thos ... | 1979 | 34919 |
| [effect of the ph of the medium on the morphology of bp cell cultures and their sensitivity to the foot-and-mouth disease virus]. | | 1979 | 34920 |
| [acid-base equilibrium in mother and fetus during labor and the resistance of newborn calves]. | investigated were a total of 59 cows presenting normal processes, of giving birth as well as dystocia, the time of delivery lasting 2, 10, 24, and 36 hours. seventeen cows with dystocia were treated intravenously with 500 cm3 of a 4 per cent solution of sodium bicarbonate and 40 g glucose. it was found that the changes in the ph values, alkali reserves, glycogen, and the peroxydase activity of the neutrophiles in the mothers and fetuses were dependent on the duration of parturition. cases of dif ... | 1978 | 34922 |
| [comparative study of the antiviral properties of histones of animal and plant origin]. | the antiviral properties of histones of animal (thymus) and plant (french beans) origin were studied in plants and with a plant virus, tobacco mosaic virus (tmv). histones of the thymus and french beans were shown to be able to inhibit tmv reproduction. the antiviral properties of histones were found to depend on their concentration, ph, and to be determined by the modes of their introduction into leaves. the manifestation of the antiviral properties of histones seems to require not only their d ... | 1978 | 34928 |
| differential properties of human chorionic gonadotrophin and human luteinizing hormone binding to plasma membranes of bovine corpora lutea. | plasma membranes of bovine corpora lutea contain common receptor sites for [125i]human chorionic gonadotrophin (hcg) and [125i]human luteinizing hormone (hlh) to which hlh binds with 4-fold lower affinity than hcg. the presence of additional sites for hlh was indicated by the lack of saturation of [125i]hlh binding as compared to [125]hcg and lower degree of inhibition of binding by 830 pm of unlabelled hcg, when [125i]hlh instead of [125i]hcg was used. differences in [125i]hcg and [125i]hlh bin ... | 1979 | 34964 |
| a simple and sensitive fluorescence assay for tyrosine hydroxylase activity. | | 1979 | 35037 |
| purification and partial characterization of beef liver gluconolactonase. | | 1979 | 35106 |
| biosynthesis of keratan sulfate: purification and properties of a galactosyltransferase from bovine cornea. | | 1979 | 35107 |
| purification and molecular and enzymic properties of mitochondrial nadh dehydrogenase. | | 1979 | 35108 |
| evaluation of biological activity of tfx (polfa) a product obtained from calf thymus. | | 1978 | 35128 |
| antigenic and immunogenic properties of gamma-glutamyl-transferase preparations. | incubation time of human kidney gamma-glutamyl-transferase (ggt) with specific antiserum and precipitate formation caused no effect on inhibition of the enzyme activity. in the obtained precipitate ggt activity was independent of the amount of antiserum and contained 30% of the initial enzyme activity. after reaction of the serum with human liver ggt or with the urine enzyme a precipitate formation and decrease in the enzyme activity was noted. in the serum incubated with ggt isolated from anima ... | 1978 | 35129 |
| [comparative investigations of the binding of perazine dimalonate (pdm) to human and bovine serum albumin (author's transl)]. | | 1979 | 35132 |
| [glucose, lactate and actual ph values in cows and calves during and immediately following birth]. | | 1979 | 35149 |
| the adsorption of proteins and protein--dodecyl sulphate complexes on n-(3-carboxypropionyl)aminodecyl-sepharose. | equilibrium and kinetic aspects of the binding of several proteins to n-(3-carboxypropionyl)aminodecyl-sepharose, an amphiphilic ampholytic adsorbent, were studied at 22 degrees c, ph 7.0, i 0.10--0.12. in the absence of detergents scatchard plots are linear for human haemoglobin and soya-bean trypsin inhibitor, but non-linear for bovine serum albumin, which is also adsorbed more tightly than the other two proteins. the introduction [corrected] of 3.5mm-sodium dodecyl sulphate causes dramatic in ... | 1979 | 35151 |
| kinetic studies of dogfish liver glutamate dehydrogenase. | initial-rate studies were made of the oxidation of l-glutamate by nad+ and nadp+ catalysed by highly purified preparations of dogfish liver glutamate dehydrogenase. with nad+ as coenzyme the kinetics show the same features of coenzyme activation as seen with the bovine liver enzyme [engel & dalziel (1969) biochem. j. 115, 621--631]. with nadp+ as coenzyme, initial rates are much slower than with nad+, and lineweaver--burk plots are linear over extended ranges of substrate and coenzyme concentrat ... | 1979 | 35153 |
| zinc(ii) binding to apo-(bovine erythrocyte superoxide dismutase). | the binding of zinc(ii) ions to apo-(bovine erythrocytes superoxide dismutase) was studied by 1h n.m.r. spectroscopy. two zinc(ii) ions bind to each subunit of the apoenzyme, and the first has a binding constant at least an order of magnitude larger than the second. the nature of the spectral changes that occur on binding the first zinc(ii) ion are interpreted in terms of a change in the structure of the protein around the active site to one very similar to that of the holoenzyme, thus pre-formi ... | 1979 | 35154 |
| effects of h+ and total carbonate concentrations on blood calcium ion level and influence of albumin. an in vitro study [proceedings]. | | 1978 | 35178 |
| ph dependence of solvent proton relaxation in carbonic anhydrase solutions: paramagnetic and diamagnetic effects. | | 1979 | 35220 |
| relaxation studies of enzymes: rapid isomerization in deoxyribonuclease i. | temperature-jump relaxation studies in deoxy-ribonuclease i were carried out at 10 degrees c and [i] = 0.1 m. the single observed relaxation time, which varied from 10(-4) to 10(-5) s, was characterized as a function of enzyme concentration, ph, and indicator concentration. the concentration and ph dependences of the relaxation time are in quantitative agreement with a mechanism involving an isomerization of the enzyme coupled to a rapid proton ionization process. the best fit forward and revers ... | 1979 | 35222 |
| hydrophobic interactions of dna with long-chain amines. | | 1979 | 35254 |
| diastereoisomeric glucuronides of oxazepam. isolation and stereoselective enzymic hydrolysis. | oxazepam glucuronide isolated from swine urine by previously published methods was separated into its diastereoisomers by ion-exchange chromatography on a preparative scale. quantitative high-performance liquid chromatography was used to monitor the separation. the two isomers were obtained in analytically pure form and then characterized by elemental analysis, oxazepam content, mass spectrometry, ultraviolet spectroscopy, optical rotation and optical rotatory dispersion-circular dichroism. the ... | 1979 | 35325 |
| kinetic analysis of the interaction of the phosphatidylcholine exchange protein with unilamellar vesicels and multilamellar liposomes. | the mode of action of the phosphatidylcholine exchange protein from bovine liver has been studied by using unilamellar vesicles and multilamellar liposomes both of which membranes contain phosphatidylcholine and phosphatidic acid. the protein-mediated exchange of phosphatidylcholine between vesicles and liposomes fit the kinetic model presented in a previous study [v.d. besselaar et al. (1975) biochemistry, 1j, 1852]. kinetic analysis of the rates of exchange indicate that the apparent dissociat ... | 1979 | 35349 |
| crosslinking of alpha-crystallin with bisimidoesters. evidence for polyamidine formation at ph8 from an increase in positive charges on the polypeptide chains. | | 1979 | 35368 |
| enzymology of butyrate formation by butyrivibrio fibrisolvens. | butyrivibrio fibrisolvens is a major butyrate-forming species in the bovine and ovine rumen. the enzymology of butyrate formation from pyruvate was investigated in cell-free extracts of b. fibrisolvens d1. pyruvate owas oxidized to acetylcoenzyme a (coa) in the presence of coa.sh and benzyl viologen or flavin nucleotides. the bacterium uses thiolase, beta-hydroxybutyryl-coa dehydrogenase, crotonase, and crotonyl-coa reductase to form butyryl-coa from acetyl-coa. reduction of acetoacetyl-coa to b ... | 1979 | 35524 |
| biotransformation of cholesterol to cholestane-3beta,5alpha,6beta-triol via cholesterol alpha-epoxide (5alpha,6alpha-epoxycholestan-3beta-ol) in bovine adrenal cortex. | | 1979 | 35533 |
| detection and characterization of circulating immune complexes by ultracentrifugation. technical aspects. | | 1979 | 35570 |
| type i interferon induced in mice by infection with trypanosoma equiperdum. | type ii interferon is known to be produced in mice infected with trypanosoma cruzi. therefore, interferon production was investigated in mice infected with trypanosoma equiperdum. interferon was found in the sera of mice infected with t. equiperdum from day 1 after infection until death on day 5. the maximal level of interferon appeared before parasitemia was detected, a finding showing that the endogenous interferon probably had no significant influence on the course of infection. unexpectedly, ... | 1979 | 35574 |
| acetoacetate and d-(-)-beta-hydroxybutyrate as precursors for sterol synthesis by calf oligodendrocytes in suspension culture: extramitochondrial pathway for acetoacetate metabolism. | | 1979 | 35588 |
| effects of magnesium and fluoride on ion exchange and acid resistance of enamel. | labial surfaces of bovine incisor enamel, after weak acid demineralization, were exposed for 24 h in solutions that contained trace levels of calcium as 45ca, 0.4 mm naf, and 1.0 mm mgcl2 at ph 7.0. the solutions approached saturation with apatites in the absence of naf, and saturation with fluorapatites in the presence of naf. naf greatly increased the exchange of 45ca. mgcl2 decreased 45ca exchange, but had no effect on f- exchange in the surface minerals. mgcl2 decreased, while naf increased ... | 1979 | 35595 |
| induction and properties of aryl hydrocarbon hydroxylase in bovine pancreatic ducts. | inducibility and characteristics of aryl hydrocarbon hydroxylase (ahh) in cultured bovine pancreatic ducts were studied by the fluorometric method. ahh was present and inducible in all the pancreatic ducts studied when they were exposed to 20 microgram benz[a]anthracene (ba)/ml medium. ahh activity in the control tissue ranged from 1.0 to 3.0 u/mg dna, whereas the activity in the ba-treated tissue was 4.2--28.5 u/mg dna, which resulted in the induction of 5- to 18-fold activity. at 12 hours of b ... | 1979 | 35637 |
| [our experiences with the kiel type heterolog conserved bone graft (author's transl)]. | the authors survey data in the medical literature on kiel type heterolog conserved bones and report their own experiences. forty-nine cases altogether, the authors grafted bone manufactured by the braun firm, in seven patients; a kiel type bone conserved by the method of the authors in forty-two patients; the grafts were used mostly in patients with solitaer bone cysts, in a smaller number in patients with bone cavities following impression fractures, pseudarthrosis and arthrodesis. their result ... | 1978 | 35677 |
| variable factors influencing the isolation of leptospires involving culture ingredients and testing. | | 1975 | 35790 |
| a quantitative mosquito survey of 7 villages in punjab province, pakistan with notes on bionomics, sampling methodology and the effects of insecticides. | a total of 451,337 female and male mosquitoes comprising 43 species in 9 genera were collected during a quantitative survey of 7 suburban and rural villages in the lahore area during 1976 and 1977 using larval, indoor resting, outdoor resting, biting and light trap collections at weekly intervals. culex tritaeniorhynchus was the most abundant species collected comprising 51.8% of the total specimens, followed by cx. quinquefasciatus (16.4%), cx. pseudovishnui (6.8%), an. subpictus (4.8%) and an. ... | 1978 | 35839 |
| [decontamination of hot beef carcases with organic acids (author's transl)]. | the decontamination effect of spraying hot beef carcases with solutions of lactic acid and solutions of the commercial preparation of ph liquid was studied. spraying a 0.5 per cent lactic acid solution only resulted in a significant reduction of aerobic, gram-negative and gram-positive bacterial counts on severely contaminated areas. treatment with a 0.75 per cent solution of lactic acid caused a significant decrease of almost all bacterial counts, whereas a 1 per cent lactic solution produced a ... | 1979 | 35851 |
| skin-piercing blood-sucking moths ii: studies on a further 3 adult calyptra [calpe] sp. (lepid., noctuidae). | 1. of the scarce calyptra minuticornis, c. orthograpta and c. labilis, 51, 24, and 7 adults, respectively, were observed during some 600 night inspections at over 100 sites in 1965--1967 and 1971--1977. 2. hitherto biologically completely unknown, and not recorded before in s.e. asia, the latter two species flew in or near tropical monsoon forests in hilly regions (300--600 m) of n. thailand (c. orthograpta also n. laos). c. minuticornis was found in these and in tropical evergreen and semi-ever ... | 1979 | 35931 |
| the feeding habits and ecology of the tsetse fly glossina morsitans submorsitans newstead in relation to nagana transmission in the gambia. | the source of blood meals from 174 fed glossina morsitans submorsitans newstead, captured in malaise traps in savanna woodland in the gambia, were identified by the precipitin test. warthog accounted for 90% of the meals and single bushbuck and ox feeds were identified. nagana is a major problem in the area, but contact between tsetse and livestock is reduced by restricted grazing. in this situation, warthog, with a ubiquitous distribution, appear to be major maintenance hosts for g. m. submorsi ... | 1979 | 35933 |
| cow red blood cells. ii. stimulation of bovine red cell glycolysis by plasma. | cow red cell glycolysis, which can be stimulated by a variety of purines and pyrimidines, was also found to be elevated by its own plasma. dialyzed or charcoal-treated plasma could no longer stimulate glycolysis, suggesting that the stimulating factors may be purines or pyrimidines. determination of purines or pyrimidines in plasma revealed the presence of xanthine (0.31 mum), hypoxanthine (0.60 mum), and adenosine (0.05 mum), as well as unknown compounds. a physiologic level of hypoxanthine, wi ... | 1979 | 35980 |
| studies of bovine liver glutamate dehydrogenase by analytical affinity chromatography on immobilized amp analogs. | | 1979 | 36042 |
| use of amphiphilic spin labels and whole cell isoelectric focusing to assay charge characteristics of sperm surfaces. | | 1979 | 36045 |
| the effects of calcium ions and ph on bovine prothrombin fragment 1. intrinsic fluroescence studies. | the effects of ph and ca2+ on the intrinsic fluorescence of bovine prothrombin fragment 1 were investigated to deduce the nature of protein functional groups involved in ca2+ binding to fragment 1. from ph values of 9 to 3, increasing the h3o+ concentration results in quenching of the fluorescence of fragment 1. reversible ph-titration curves are obtained which appear to consist of two regions. from ph 4 to ph6.5 a broad titration curve is obtained, whereas from ph6.5 to 9 a more pronounced titr ... | 1979 | 36074 |
| sulfoxidation of chlorpromazine and thioridazine by bovine liver--preferential metabolic pathways. | | 1979 | 36084 |
| studies on the mechanism of drug-binding to melanin. | | 1979 | 36092 |
| [specific binding of perazine, a piperazine side-chain phenothiazine drug, to a serum protein (author's transl)]. | a binding of perazine to a serum protein of 48 000 d was determined by gel filtration. the affinity constant of the perazine-protein complex was found to be 5.42 x 10(6) mol/l corresponding to a specific binding of 70 ng/ml serum. this result may gather clinical relevance with regard to the "cns-bioavailability" and individual response to perazine, the average therapeutic serum concentrations having been shown to range between appr. 50 and 200 ng/ml serum. a specific binding of perazine to human ... | 1979 | 36101 |
| antitumor drug-protein interactions. binding of 1-beta-d-arabinofuranosyl cytosine to albumin and chemically modified albumin. | equilibrium dialysis measurements were carried out to study the binding of 1-beta-d-arabinofuranosyl cytosine (ara-c) to human and bovine serum albumin (hsa, bsa) and to chemically modified albumin. the binding of 4-phenylbutyric acid to hsa was studied, too. binding data were presented as scatchard plots. there are two types of binding sites of different affinity for ara-c both on hsa and bsa. the relatively small value of affinity constant indicates that the pharmacological properties of ara-c ... | 1979 | 36105 |
| the buffering capacity of the internal phase of thylakoids and the magnitude of the ph changes inside under flashing light. | the buffering capacity inside thylakoids is determined and the magnitude of flash-induced ph changes inside is calibrated in the ph range from 6.4 to 8.1. the work is based on flash-induced absorption changes of neutral red in a chloroplast suspension in which the outer phase is strongly buffered by bovine serum albumin. it is shown that neutral red is bound inside thylakoids. the binding can be described by a simple isotherm with an apparent km = 4 microm and satruation at 1 neutral red per 17 ... | 1979 | 36138 |
| the electrogenic nature of adp/atp transport in inside-out submitochondrial particles. | | 1979 | 36139 |
| the isolation of stable cattle rod outer segments with an intact plasma membrane. | a procedure is described to purify and stabilize cattle rod outer segments with an intact plasma membrane. three criteria are applied to assess the integrity of the latter. upon photolysis in these rod outer segments: (1) exogenous atp cannot phosphorylate rhodopsin located in the disk membrane. (2) endogenous cofactors (nadph, nadph-regenerating system) are still available in the rod cytosol and consequently retinol is the final photoproduct of photolysis of rhodopsin. (3) the rod cytosol can m ... | 1979 | 36143 |
| clustering of fatty acids in phospholipid bilayers. | from electrophoresis experiments it is concluded that acidic phospholipids incorporated in liquid crystalline phosphatidylcholine bilayers at neutral ph are randomly distributed. the same is true for spin-labelled fatty acids. in contrast, long chain fatty acids are not fully ionized at neutral ph and appear to be clustered, i.e. they segregate out into patches. only at ph greater than 11 is the fatty acid-cooh group fully ionized and charge repulsion leads to a random distribution of the fatty ... | 1979 | 36148 |
| effects of atp on the intermediary steps of the reaction of the (na+ + k+)-atpase. iv. effect of atp on k0.5 for na+ and on hydrolysis at different ph and temperature. | the ph optimum for (na+ + k+)-atpase (atp phosphohydrolase, ec 3.6.1.3) depends on the combination of monovalent cations, on the atp concentration and on temperature. atp decreases the na+ concentration necessary for half maximum activation, k0.5 for na+ (na+ + k+ = 150 mm), and the effect is ph and temperature dependent. at a low atp concentration a decrease in ph leads to an increase in k0.5 for na+, while at the high atp concentration it leads to a decrease. k0.5 for atp for hydrolysis decrea ... | 1979 | 36159 |
| the sulphatase of ox liver. xxii. further observations on the cerebroside sulphatase activity of sulphatase a. | further studies have been made of the cerebroside sulphatase activity of the sulphatase a (aryl-sulphate sulphohydrolase, ec 3.1.6.1) of ox liver. it is concluded that a cerebroside sulphate-modified form of the enzyme is not produced and that the kinetics of the reaction can be explained by the utilisation of the substrate and accumulation of (so4)2-. the hypothesis is advanced that this difference between the cerebroside sulphatase and arylsulphatase activities arises from non-polar binding of ... | 1979 | 36164 |