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[rayer's studies on the contagion of glanders (1837-1843)].p. rayer (1795-1867) had never thoroughly published his experimental studies on the contagion of glanders. his recently un-earthed hand written papers allow us to depict his experimental approach and its results. he was not the first who transmitted glanders from a patient to horses or donkeys. but he did it systematically with glander secretions from acute and chronic cases. whatever was the disease of the donors the transmitted forms were unpredictably either chronic or acute. his conclusion w ...200212607548
[a case report of a donkey bite injury with avulsion of the eyelid]. 200212616955
sensing and fixation of no2/n2o4 by calix[4]arenes.an approach toward visual detection and chemical utilization of no(2)/n(2)o(4) is proposed, which employs simple calix[4]arenes. exposure of tetra-o-alkylated calix[4]arenes 1 and 2, possessing either a cone or a 1,3-alternate conformation, to no(2)/n(2)o(4), both in chloroform solution and in the solid state, results in deeply colored calixarene-nitrosonium (no(+)) complexes. in the presence of a lewis acid, such as sncl(4), stable calixarene-no(+) complexes 7 and 8 were isolated in a quantitat ...200312617667
biodiversity of domestic livestock in the republic of yemen.this paper describes the domestic livestock of the republic of yemen and aspires to complement earlier sources listing or partially describing 'breeds'. it attempts to cover all species and provide indications of production parameters through a literature review and via field observations made by the author in 1999. information is provided on livestock numbers and the economic importance of animal production. most animals are kept in sedentary mixed crop-livestock production systems; transhumant ...200312636359
[in acute pain, self-medication with ass is reliable]. 200312638447
grade iii mastocytoma in a donkey. 200312638914
phenotypic population characteristics of the enterococci in wastewater and animal faeces: implications for the new european directive on the quality of bathing waters.faecal pollution of recreational bathing waters may derive from point sources of various wastewaters or from more diffuse sources such as run-off of agricultural wastes. the paper describes the application of population similarity studies to the enterococcal flora of various animal faeces and municipal wastewaters as a means of distinguishing human from animal faecal material. a simplified phenotypic testing technique (pheneplate, php) was used to study the fermentation kinetics of eleven carboh ...200312639001
intravenous salbutamol bolus compared with an aminophylline infusion in children with severe asthma: a randomised controlled trial.the relative efficacies of aminophylline and salbutamol in severe acute childhood asthma are currently unclear. a single bolus of salbutamol was compared with a continuous aminophylline infusion in children with severe asthma in a randomised double blind study.200312668792
["screening" in special situations. assessing predictive genetic screening for hereditary breast and colorectal cancer].aims and methods: the aim of this health technology assessment was to analyse the current scientific and genetic counselling on predictive genetic testing for hereditary breast and colorectal cancer. predictive genetic testing will be available for several common diseases in the future and questions related to financial issues and quality standards will be raised. this report is based on a systematic/nonsystematic literature search in several databases (e.g. embase, medline, cochrane library) an ...200312669692
[the cloning and expression of rat insulin-like growth factor i gene].in this study an exons-connecting technique was used to amplify the complete dna sequence encoding the mature peptide of igf-i. two pairs of primers were synthesized, primer a (pa) and primer b (pb) were used to amplify exon 2 coding fragment while primer c (pc) and primer d (pd) for amplifying exon 3 coding fragment. pb was 40 bp, 18 bp at its 5' end was same with the anti-sense of exon 3's 5' end. pc consisted of 41 bp with 22 bp at its 5' end consistent with the sense of the exon 2's 3' end. ...200212693096
[quality of diagnostic procedures and frequency of endoscopically defined diseases of the upper gastrointestinal tract].the aim of this clinical investigation was to register the frequency of endoscopically defined diseases of the upper intestinal tract in a given region (münster and münsterland) within the period of one year (1.8.1999-31.7.2000). furthermore, we tried to get an impression on the quality of the upper intestinoscopies by standardised conditions which had been developed by a steering committee (endoscopists and pathologists). 20 physicians (internal specialists and gastroenterologists) examined non ...200312695936
argininosuccinate synthetase from the urea cycle to the citrulline-no cycle.argininosuccinate synthetase (ass, ec 6.3.4.5) catalyses the condensation of citrulline and aspartate to form argininosuccinate, the immediate precursor of arginine. first identified in the liver as the limiting enzyme of the urea cycle, ass is now recognized as a ubiquitous enzyme in mammalian tissues. indeed, discovery of the citrulline-no cycle has increased interest in this enzyme that was found to represent a potential limiting step in no synthesis. depending on arginine utilization, locati ...200312709047
measurement of spin-correlation parameters ann, ass, and asl at 2.1 gev in proton-proton elastic scattering.at the cooler synchrotron cosy/jülich spin-correlation parameters in elastic proton-proton (pp) scattering have been measured with a 2.11 gev polarized proton beam and a polarized hydrogen atomic beam target. we report results for a(nn), a(ss), and a(sl) for c.m. scattering angles between 30 degrees and 90 degrees. our data on a(ss)--the first measurement of this observable above 800 mev--clearly disagrees with predictions of available pp scattering phase-shift solutions while a(nn) and a(sl) ar ...200312731910
adrenocortical carcinoma in a donkey.an adrenocortical carcinoma was found in an eight-year-old male donkey. the main clinical feature was severe depression and inappetence. the animal died 24 hours after hospitalization. necropsy revealed a large tumor around the anterior pole of the left kidney and metastases to liver, heart and lung. fibrous adhesions were seen between the tumor and the intestinal serosa. the abdominal cavity contained a large amount of red-brown fluid.200312756961
refilling of cortical calcium stores in paramecium cells: in situ analysis in correlation with store-operated calcium influx.this is the first thorough study of refilling of a cortical calcium store in a secretory cell after stimulation in which we combined widely different methodologies. stimulation of dense-core vesicle ("trichocysts") exocytosis in paramecium involves a ca(2+) -influx" superimposed to ca(2+) -release from cortical stores ("alveolar sacs" (ass)). in quenched-flow experiments, membrane fusion frequency rose with increasing [ca(2+)](o) in the medium, from approximately 20-25% at [ca(2+)](o) < or = 0.2 ...200312767896
equine telomeres and telomerase in cellular immortalisation and ageing.to determine the role of telomeres in cellular ageing in equids, we analysed telomere lengths in peripheral blood derived dna samples from a panel of donkeys (equus asinus) ranging from 2 to 30 years of age. the average telomere lengths ranged from 7 to 21 kbp and a statistically significant inverse correlation between telomere lengths and donor age was demonstrated. similarly, telomere lengths in primary fibroblasts isolated from a horse (equus equus) demonstrated telomeric loss with in vitro a ...200312782419
diagnosis and treatment of arterial steal syndromes in liver transplant recipients.arterial steal syndromes (asss) after orthotopic liver transplantation (olt) are characterized by arterial hypoperfusion of the graft caused by shifting of blood flow into the splenic or gastroduodenal artery. despite their potentially devastating consequences, such as ischemic biliary tract destruction or graft failure, asss have received little attention to date. we report the incidence, diagnosis, and treatment of ass among 1,250 consecutive olts. ass was observed in 69 patients (5.9%). all t ...200312783401
effectiveness of carbohydrate-restricted diet and arginine granules therapy for adult-onset type ii citrullinemia: a case report of siblings showing homozygous slc25a13 mutation with and without the disease.case 1 is a 37-year-old japanese man who was admitted to this hospital with a disturbance of consciousness. a diagnosis of adult-onset type ii citrullinemia was made by dna analysis of slc25a13 (851del4/851del4) and measurement of hepatic ass activity (2% of control value). after regaining consciousness, case 1 was started on a dietary therapy, revealing that hypertriglyceridemia and ketogenesis impairment deteriorated on a low-protein diet and ameliorated on a carbohydrate-restricted (high-prot ...200312787807
thoracic intervertebral disc protrusion in a donkey. 200312790240
the prevalence and transmission to exotic equids (equus quagga antiquorum, equus przewalskii, equus africanus) of intestinal nematodes in contaminated pasture in two wild animal parks.wild equids maintained in large enclosures may suffer from helminth diseases because common hygiene practices have only limited effects on parasite populations. weekly monitoring of helminth prevalences and pasture infestation was performed for 1 yr in several extensive maintenance systems of two wildlife parks with similar climates to determine when veterinary intervention to control parasites would be useful. we also sought evidence of natural immunogenic reactions among herds of chapman zebra ...200112790423
[local therapy with capsaicin or ass in chronic pain].to provide a brief review of the current state of topical treatment with capsaicin or acetylsalicylic acid (asa) for therapy of chronic pain syndromes.199712799806
the chemotherapy of rodent malaria. lxi. drug combinations to impede the selection of drug resistance, part 4: the potential role of 8-aminoquinolines.the influence of combinations containing the blood schizontocides chloroquine (cq) or mefloquine (mef), together with the 8-aminoquinolines (8aq) primaquine (pq) or the new, long-acting compound, tafenoquine (taf), on the rate of selection of resistance to the individual compounds was examined using the asexual, intra-erythrocytic stages in rodent malaria models. the two main procedures used were a 'serial technique' (st) and the '2%- relapse technique' (2%rt). the st provided evidence for the c ...200312803854
identification of 16 novel mutations in the argininosuccinate synthetase gene and genotype-phenotype correlation in 38 classical citrullinemia patients.classical citrullinemia (ctln1), a rare autosomal recessive disorder, is caused by mutations of the argininosuccinate synthetase (ass) gene, localized on chromosome 9q34.1. ass functions as a rate-limiting enzyme in the urea cycle. previously, we identified 32 mutations in the ass gene of ctln1 patients mainly in japan and the united states, and to date 34 different mutations have been described in 50 families worldwide. in the present study, we report ass mutations detected in 35 additional ctl ...200312815590
setaria equina infection of turkish equines: estimates of prevalence based on necropsy and the detection of microfilaraemia.necropsies on 43 horses, 35 donkeys and two mules slaughtered in ankara, turkey, revealed that 12 (15%) of the equines harboured adult setaria equina. when blood samples were checked for microfilariae, using knott's method and a combination of membrane filtration followed by histochemical staining for acid phosphatase (ap), only three (4%) of the animals were found to be microfilaraemic. when stained for ap, the s. equina microfilariae exhibited diffuse red staining over the entire body, includi ...200312831525
seasonal fluctuation of parasitic infestation in donkeys (equus asinus) in oodi village, kgatleng district, botswana.during the period march to september 2000, a study was conducted in oodi village, kgatleng district, botswana, to investigate the seasonal fluctuation of internal, external and blood parasites of donkeys. twelve adult donkeys were randomly selected from a farmer with a herd of 15 donkeys. monthly visits were made to the farmer when the donkeys were examined for parasites. the only ectoparasites recovered from the donkeys were instars of various tick species. the most prevalent tick was rhipiceph ...200312836744
heparin and calcium ions dramatically enhance antithrombin reactivity with factor ixa by generating new interaction exosites.blood coagulation factor ixa has been presumed to be regulated by the serpin, antithrombin, and its polysaccharide activator, heparin, but it has not been clear whether factor ixa is inhibited by the serpin with a specificity comparable to that for thrombin and factor xa or what determinants govern this specificity. here we show that antithrombin is essentially unreactive with factor ixa in the absence of heparin (k(ass) approximately 10 m(-1) s(-1)) but undergoes a remarkable approximately 1 mi ...200312846563
developmental dynamics of the bronchial (airway) and air sac systems of the avian respiratory system from day 3 to day 26 of life: a scanning electron microscopic study of the domestic fowl, gallus gallus variant domesticus.the lung buds were first conspicuous on day 3 of embryogenesis. they fused on day 4 and the common growth divided into left and right primordial lungs on day 5. progressively, the lungs elongated, diverged, and advanced towards the respective dorsolateral aspects of the body wall, reaching their definitive topographical locations in the coelomic cavity on day 6. on day 7, they rotated, attached onto the ribs, gradually started to slide into them, and were deeply inserted by day 8. the primary br ...200312856178
in vitro infection of nonendothelial cells by ehrlichia ruminantium.the welgevonden stock of ehrlichia ruminantium was propagated in eight nonendothelial cell cultures derived from different animal species, both ruminants and nonruminants. the origins of the cells were: bovine fetal testis (bft), cat ovary (coc), donkey fibroblasts (dfc), sheep fibroblasts (e(2)), horse testis (htc), lamb fetal testis (lft), mouse connective tissue (l), and african green monkey kidney (vero). four cell culture types (bft, e(2), lft and vero) required supplementation of the mediu ...200312860692
[expression and immunogenicity of equine infectious anemia virus membrane protein gp90].membrane protein gp90 of china equine infectious anemia virus (eiav) vaccine strain (dlv) and its parental wild type ln strain were expressed with bac-to-bac baculovirus expression system and balb/c mice were inoculated with purified protein, thereby to explore the availability of protein for differential diagnosis and potential for preparing genetically engineered vaccine.200312870025
[perioperative management of a patient with sneddon syndrome--a case report].sneddon's syndrome is a rare combination of generalised livedo reticularis and cerebrovascular accidents. its clinical presentation varies widely and its aetiology is still not known. 60 to 80% of patients are female. first symptoms of the syndrome are mostly repetitive cerebral strokes, but reduced perfusion of the skin, seen as blue or red-brown mottling, precedes the strokes. the vascular disease is generalised and often accompanied by arteriosclerosis, systemic arterial hypertension, valvula ...200312872540
[histomorphometric study of bone microstructure of primates and domestic animal with the goal of species identification with reference to the effects of domestication].functional bone microstructure of long limb bones is a function of species-specific biomechanical properties such as locomotion and weight. histomorphometry and statistics were used to identify various primate species (hylobates moloch, pongo satyrus borneensis, pan tr. troglodytes, gorilla g. gorilla, homo sapiens), equid species (equus caballus, equus asinus, equus mulus, equus hemionus kulan, equus ferus przewalskii) and also extinct horses e.g. iron age, medieval and neolithic forms on the m ...200312872544
co-detection of pth/pthrp receptor and tartrate resistant acid phosphatase in osteoclasts.serial sections of rat metaphyses were prepared from paraffin embedded tissue blocks and analyzed in sets of three. the central section was stained for tartrate resistant acid phosphatase (trap) in order to identify osteoclasts, one adjacent section was immunostained with an affinity purified antibody to a 15 amino acid sequence unique to rat pth/pthrp receptor, and the other adjacent section in the set served as an immunostaining control. this allowed each of the 110 osteoclasts examined to be ...200312874824
determination of some micro- and macroelements in preparations made from peppermint and nettle leaves.as, ca, cd, cu, fe, mg, mn, pb and zn were determined by icp-ms and et-aas in preparations made from peppermint and nettle leaves after microwave high pressure digestion with nitric acid. in preparation from nettle leaves the ca content was more than three times higher than in preparations made from peppermint leaves. only very small differences (less than 10%) were observed for all the other elements. in all the samples investigated the content of inorganic elements were below the who limit (wh ...200312889531
recombinant sindbis/venezuelan equine encephalitis virus is highly attenuated and immunogenic.venezuelan equine encephalitis virus (veev) is an important, naturally emerging zoonotic virus. veev was a significant human and equine pathogen for much of the past century, and recent outbreaks in venezuela and colombia (1995), with about 100,000 human cases, indicate that this virus still poses a serious public health threat. the live attenuated tc-83 vaccine strain of veev was developed in the 1960s using a traditional approach of serial passaging in tissue culture of the virulent trinidad d ...200312915543
linguistic processing in idiopathic generalized epilepsy: an auditory event-related potential study.auditory processing of increasing acoustic and linguistic complexity was assessed in children with idiopathic generalized epilepsy (ige) by using auditory event-related potentials (aerps) as well as reaction time and performance accuracy.200312919393
measurements of sulfur in oil using a pressurised wet digestion technique in open vessels and isotope dilution mass spectrometry.graz university of technology has developed a new technique for digesting samples using the well-established high-pressure asher (hpa) from anton paar gmbh (graz, austria). the digestion is performed in semi-open vessels inside a pressurised autoclave. the new hpa equipment consists of a liner for the autoclave, special sample racks and 30-ml digestion vessels made of quartz, covered with ptfe stoppers. the laboratory for isotope dilution and nuclear analysis of the federal institute for materia ...200312925866
cloning and characterization of a gene encoding an immunoglobulin-binding receptor on the cell surface of some members of the family trypanosomatidae.several members of the trypanosomatidae family, when freshly isolated from their mammalian hosts, have immunoglobulins adsorbed to their cell surfaces. however, a significant portion of these antibody molecules is not parasite specific, i.e., the immunoglobulins are bound to the parasite's cell surface molecules via noncognitive interactions. it has been proposed that this noncognitive adsorption of immunoglobulins to the parasite is mediated by an fc-like receptor present in several members of ...200312933849
vertebral body hemangiosarcoma in a 16-year-old miniature sicilian donkey. 200312939060
severe infestation of a she-ass with the cat flea ctenocephalides felis felis (bouche, 1835).a 6-year-old she-ass was found to be severely infested with the cat flea ctenocephalides felis felis. the animal was severely anaemic and after 2 days of recumbency, it succumbed to the flea infestation. the acrimonious anaemia was characterised as normocytic, hypochromic.200312944051
in situ uv-vis detection of the association of water-soluble anionic porphyrin and aromatic bases in aqueous solution at high tempeatures using a capillary flow hydrothermal reactor system.the association between anionic water-soluble porphyrin and aromatic heterocyclic bases has been investigated to demonstrate the usefulness of a new in situ uv-vis monitoring method of hydrothermal reactions. spectral and absorbance changes in the presence of bases were possible to be detected by the new method at temperatures up to 150 degrees c. the association constants (k(ass,1)) were determined from the absorbance-base concentration curves. the logarithmic values of k(ass,1) were proportion ...200312945678
successful non-surgical transfer of horse embryos to mule recipients.mules, hybrids resulting from the mating of a horse mare (equus caballus, 2n = 64) to a jack donkey (e. asinus, 2n = 62), are generally infertile. five horse embryos were transferred non-surgically to two cyclic and one acyclic recipient mules. in the mares and cycling mules, oestrus and ovulation were induced with, respectively, d-cloprostenol and human chorionic gonadotrophin (hcg). the acyclic mule, on the other hand, received oestradiol benzoate when the embryo donor was showing oestrus and ...200312950689
new approaches to overcome tolerance to nitrates.in isolated cells (vascular smooth muscle, endothelium, platelets), perfused hearts, in vivo experiments, conscious instrumented animals, and in human subjects the induction of tachyphylaxis and tolerance to various exogenous no-donors was analyzed. various ways to circumvent tolerance were successfully tested. different nitrovasodilators were associated with different rates and magnitudes of generation of tolerance and reactive oxygen radicals (ros) in all models tested, beginning with petn (pe ...200312975598
some blood values of the southwestern burro (equus asinus asinus). 195212996771
the lethal dose of whole-body tantalum182 gamma irradiation for the burro (equus asinus asinus). 195313038055
[pathology of metabolism of fats; an extraordinary composition of fatty acids of liver glyceride of donkey]. 195213044145
effect of 400 fractional whole body gamma-irradiation in the burro (equus asinus asinus). 195313065497
[blood groups in equidae. iii. blood groups in donkey]. 195313125006
hematological response of the burro (equus asinus asinus) to total body tantalum-182 irradiation. 195413217664
the conglutination phenomenon. x. conglutinin and immuno-conglutinin in guinea-pigs, pigs, donkeys, rats and mice. 195413221820
immunological relation of donkey, mule and horse serum albumin. 195513301490
lungworm disease in ponies; a case report of the respiratory worm parasitism in ponies and a donkey. 195713437696
[on the inactivation of oxytocin in bos taurus, equus caballus, equus asinus, ovis aries, canis familiaris, sus scrofa, lepus cuniculus, cavia cobaya, mus norvegicus albinus, mus musculus, callus gallus and columba livia]. 196113766350
the mortality response of the burro (equus asinus) to a single total-body exposure of gamma radiations from zr95/nb95. 195913839566
coccidial oocysts (eimeria leuckarti) from north american donkeys (asinus asinus). 196213867053
somatic chromosomes of the horse, the donkey and their hybrids, the mule and the hinny. 196213970509
chromosomes of the horse, the donkey, and the mule. 196213994375
[a further contribution to the knowledge of placentas of the perissodactyla order: a full-term placenta of equus asinus l]. 196314042443
ecological change as a factor in renewed malaria transmission in an eradicated area. a localized outbreak of a. aquasalis-transmitted malaria on the demerara river estuary, british guiana, in the fifteenth year of a. darlingi and malaria eradication.in british guiana, the successful eradication of anopheles darlingi and malaria from the coastal areas has caused a very rapid increase in the population and has favoured a considerable social and economic improvement and expansion of both agriculture and industry. housing and industrial developments and the constantly expanding rice cultivation have taken over most of the accessible pasture-lands, displacing the livestock which previously abounded around villages and settlements. mechanization ...196314056265
[eeg and behavioral study of alternating waking and sleeping in the donkey]. 196314081745
comparative examination of carboxylic esterases in sera of horse, donkey and their hybrids. 196414134727
chromosome studies of a donkey-grevy zebra hybrid. 196414171168
sex-linkage of glucose-6-phosphate dehydrogenase in the horse and donkey.distinctly different electrophoretic patterns of red cell glucose-6-phosphate dehydrogenase were resolved from the hemolyzates of horse and donkey erythrocytes. examination of their reciprocal hybrids, mules and hinnies, showed that the red cells of female mules and female hinnies contain both horse and donkey g-6-pd; the male mule with an x chromosome from its horse mother contained pure horse g-6-pd, whereas the male hinny with the donkey x chromosome contained pure donkey g-6-pd. these findin ...196514287603
myopathy in a donkey. 196514331454
a note on the presence of glandular acini and tubules in the bladder and ureter of a donkey. 196514344276
[observations on the morphological elements of the prelymph node pulmonary lymph of some perissodactyla: equus caballus, equus asinus, equus caballus-equus asinus]. 196014441908
the hybridization of donkey and mouse hemoglobins. 196214492238
[pulsimetry].pulsimetry is a non-invasive technique to monitor the saturation of oxygen in the hemoglobin of blood in arteries. initially. this was used by anesthesiologists to control oxygen levels in patients undergoing general anesthesia and in the care of critically ill patients. given its effectiveness to detect precociously situations of hypoxia, its use has rapidly expanded during the decade of the '90s to other units having patients who need a correct measure of their respiratory functions. as with a ...200214506767
endocrinology: nitric oxide-mediated insulin secretion in response to citrulline in islet beta-cells.nitric oxide (no) synthases (noss) are expressed in insulin secreting beta-cells. however, physiologic role of no in insulin release is still controversial. we previously reported that argininosuccinate synthetase (ass) and argininosuccinate lyase (asl), together with nos, constitute the citrulline-argininosuccinate-arginine (cit-as-arg) cycle in beta-cells and that this cycle metabolizes citrulline to produce no and increase cytosolic ca2+ concentration ([ca2+]i) in islet beta-cells.200314508123
using the expolinear growth equation for modelling crop growth in year-round cut chrysanthemum.the aim of this study was to predict crop growth of year-round cut chrysanthemum (chrysanthemum morifolium ramat.) based on an empirical model of potential crop growth rate as a function of daily incident photosynthetically active radiation (par, mj m-2 d-1), using generalized estimated parameters of the expolinear growth equation. for development of the model, chrysanthemum crops were grown in four experiments at different plant densities (32, 48, 64 and 80 plants m-2), during different seasons ...200314514517
yeast beta-alanine synthase shares a structural scaffold and origin with dizinc-dependent exopeptidases.beta-alanine synthase (beta as) is the final enzyme of the reductive pyrimidine catabolic pathway, which is responsible for the breakdown of pyrimidine bases, including several anticancer drugs. in eukaryotes, beta ass belong to two subfamilies, which exhibit a low degree of sequence similarity. we determined the structure of beta as from saccharomyces kluyveri to a resolution of 2.7 a. the subunit of the homodimeric enzyme consists of two domains: a larger catalytic domain with a dizinc metal c ...200314534321
visceral leishmaniasis in eastern sudan: parasite identification in humans and dogs; host-parasite relationships.in 1996, an epidemic outbreak of visceral leishmaniasis (vl) started in barbar el fugara, a village in gedarif state (eastern sudan). from 1997 to 2000, regular epidemiological studies were carried out in the human population, as well as in mammals and sand flies. in symptomatic patients, 46/69 lymph node, 6/20 post kala-azar dermal leishmaniasis (pkdl) and 1/4 cutaneous cultures in nnn medium were positive. in 69 dogs, 23/79 lymph node cultures were positive. in other mammals (47 rodents, five ...200314554251
soil amino acid composition quantified by acid hydrolysis and anion chromatography-pulsed amperometry.soil organic n accounts for 95-98% of the total soil n content with amino acids (aas) and amino sugars (ass) identified as the major soil organic n compounds, but traditional 6 m hcl with reflux or sealed digestions for 24 h and various detection systems have accounted for only 30-40% of soil total n content as aa-n. this study compared traditional hcl extraction methodology with methanesulfonic acid (msa) hydrolysis and nonderivatized aa and as quantification by ion chromatography with pulsed a ...200314558773
aging and synaptic changes in the paraventricular hypothalamic nucleus of the rat.the age-related differences in the synapses of the paraventricular hypothalamic nucleus (pvn) were studied with transmission electron microscopy in young (3-month-old) and senescent (25-month-old) wistar rats. the changes in the axodendritic (ads) and axospinous (ass) synapses in the pvn neuropil were determined by morphometry. the majority of synapses were of the ads type. in senescent rats the density of both ads and ass was diminished. the mean area of the ass presynaptic boutons in aged rats ...200314570152
glutamine stimulates argininosuccinate synthetase gene expression through cytosolic o-glycosylation of sp1 in caco-2 cells.glutamine stimulates the expression of the argininosuccinate synthetase (ass) gene at both the level of enzyme activity and mrna in caco-2 cells. searching to identify the pathway involved, we observed that (i) the stimulating effect of glutamine was totally mimicked by glucosamine addition, and (ii) its effect but not that of glucosamine was totally blocked by 6-diazo-5-oxo-l-norleucine (don), an inhibitor of amidotransferases, suggesting that the metabolism of glutamine to glucosamine 6-phosph ...200314570901
[ass and dipyridamole combination. a decisive contribution to secondary prevention after stroke]. 200314579496
[secondary prevention of ischemic insult and transient cerebral ischemia. is ass alone enough?]. 200314579505
antigenic analysis of nonstructural protein (nsp) 4 of group a avian rotavirus strain po-13.in order to analyze the antigenic structure of nonstructural protein (nsp) 4 of group a avian rotavirus strain po-13, 25 monoclonal antibodies (mabs) against nsp4 expressed in escherichia coli were produced. all mabs reacted with nsp4 on western blotting, indicating that they recognized sequential epitopes. to determine the antigenic sites (ass) recognized by the produced mabs, seven truncated nsp4s were expressed in e. coli. western blotting analysis showed that there are at least four major as ...200314584613
[dual platelet inhibition with clopidogrel and ass. who profits from the combination?]. 200314587200
use of magnetic motor-evoked potentials in horses with bilateral hind limb ataxia.to determine the usefulness of magnetic motor-evoked potentials (mmeps) for assessing the integrity of the cervical, thoracic, and thoracolumbar spinal cord in horses with bilateral hind limb ataxia.200314620774
inter- and intramolecular fluorescence quenching of organic dyes by tryptophan.steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. especially, the red-absorbing dyes atto 655, atto 680, and the oxazine derivative mr 121 are strongly quenched almost exclusively by tryptophan due to the formation of weak or nonfluorescent ground-state complexes ...200314624626
acquired resistance of horses to amblyomma cajennense (fabricius, 1787) ticks.acquired immunity of horses to larvae, nymphs and adults of the amblyomma cajennense tick was evaluated through three consecutive experimental infestations of tick-bite naïve hosts. data from these infestations were compared to those from field-sensitized horses and donkeys. it was observed that tick-bite naïve horses developed a low level of resistance after two infestations as shown by a significant decrease in larval yield and a tendency for lower engorged weight of nymphs during third infest ...200314637029
recent advances in molecular epidemiology and detection of taylorella equigenitalis associated with contagious equine metritis (cem).in the present review article, recent molecular advances relating to studies with taylorella equigenitalis, as well as the recently described second species of the genus taylorella, namely taylorella asinigenitalis, have been described. molecular genotyping of t. equigenitalis strains by pulsed-field gel electrophoresis (pfge) after digestion with the suitable restriction enzyme(s) enabled the effective discrimination of strains, thus allowing the examination of the scientific mechanism(s) for i ...200314637043
the amino acid sequence of protein aa from a burro (equus asinus).the primary structure of amyloid fibril protein aa of a burro has been determined by edman degradation. the 80 amino acid residue long protein shows strong resemblance to that of other mammalian aa-proteins and differs from equine protein aa at 5 positions: burro/horse positions 20 (q/n), 44 (r,q, k/k,q), 59 (g,l/g,a), 61 (q/e) and 65 (n/r).200314640027
life-threatening hemorrhage from enterotomies and anastomoses in 7 horses.to report our experience with horses that presumptively had severe intraluminal hemorrhage from enterotomy or anastomosis.200314648534
[therapy with nonsteroidal anti-inflammatory drugs].nonsteroidal anti-inflammatory drugs (nsaids) are among the most frequently prescribed drugs worldwide. their analgetic and antiphlogistic effect results from an efficient inhibition of prostaglandin synthesis. the risk of side effects is remarkable. this concerns namely side effects in the gastrointestinal tract and the kidneys. the common nsaids inhibit both isoforms of the cyclooxygenase (cox). the analgetic and antiphlogistic effect results from the inhibition of cox-2, whereas the inhibitio ...200314655005
in-vivo therapeutic efficacy trial with artemisinin derivative, buparvaquone and imidocarb dipropionate against babesia equi infection in donkeys.the therapeutic efficacy of imidocarb, artesunate, arteether, buparvaquone and arteether+buparvaquone combination was evaluated against babesia equi of indian origin in splenectomised donkeys with experimentally induced acute infection. efficacies of these drugs were tested by administering each drug or drug combination to groups of donkeys (having three donkeys each group). one group of donkey was kept as untreated control for comparing the results. parasitaemia, haematology (wbc, rbc, pcv, gra ...200314665744
[blood feeding sources and peridomiciliation of lutzomyia longipalpis (lutz & neiva, 1912) (psychodidae, phlebotominae)].a precipitin test was employed to study the alimentary tract content of lutzomyia longipalpis in the intra- and peridomiciliary environments in the municipality of raposa, maranh o state, a transmission area for visceral leishmaniasis or kala azar. out of 2,240 female sandflies captured, 547 (24.4%) had fed on vertebrate blood, with the following proportions: avian (87.9%); rodent (47.2%); human (42.4%); canine (27.6%); opossum (26.6%); and equine (22.5%). based on a survey of 120 human dwelling ...200314666218
peptide fragments of the dihydropyridine receptor can modulate cardiac ryanodine receptor channel activity and sarcoplasmic reticulum ca2+ release.we show that peptide fragments of the dihydropyridine receptor ii-iii loop alter cardiac ryr (ryanodine receptor) channel activity in a cytoplasmic ca2+-dependent manner. the peptides were ac (thr-793-ala-812 of the cardiac dihydropyridine receptor), as (thr-671-leu-690 of the skeletal dihydropyridine receptor), and a modified as peptide [as(d-r18)], with an extended helical structure. the peptides added to the cytoplasmic side of channels in lipid bilayers at > or = 10 nm activated channels whe ...200414678014
mild citrullinemia in caucasians is an allelic variant of argininosuccinate synthetase deficiency (citrullinemia type 1).citrullinemia is caused by either deficiency of argininosuccinate synthetase (ass, citrullinemia type 1) or a defect of the slc25a13 gene encoding a mitochondrial aspartate-glutamate transporter (citrullinemia type ii). citrullinemia type 1-referred to as classical citrullinemia-is characterized by largely elevated concentrations of citrulline, manifesting with acute hyperammonemic crises predominantly early in life and occurs panethnically. citrullinemia type ii is a rare multisystem-disorder n ...200314680976
fixed nucleotide differences on the y chromosome indicate clear divergence between equus przewalskii and equus caballus.the phylogenetic relationship between equus przewalskii and e. caballus is often a matter of debate. although these taxa have different chromosome numbers, they do not form monophyletic clades in a phylogenetic tree based on mtdna sequences. here we report sequence variation from five newly identified y chromosome regions of the horse. two fixed nucleotide differences on the y chromosome clearly display przewalski's horse and domestic horse as sister taxa. at both positions the przewalski's hors ...200314687077
ultrastructure of junction areas between neurons and astrocytes in rat supraoptic nuclei.to determine the ultrastructure of junction areas between neurons and astrocytes of supraoptic nuclei in rats orally administered 30 g/l nacl solution for 5 days.200414695781
purification and crystallization of haemoglobin from donkey (equus asinus).haemoglobin acts as an important protein for oxygen carrier in all living beings. purification of donkey haemoglobin was done using peg 4000 as a separating medium. crystallization was achieved using hanging drop vapor diffusion method using 2.8 m phosphate buffer, ph 6.8. data collection was done using mar345 image plate detector system. the crystals grown under 2.8 m phosphate buffer are monoclinic with space group c2 and cell dimensions a=107.664 a, b=63.084 a, c=54.042 a, and beta=111.747 de ...200414697211
a novel tricolor, dual-fusion fluorescence in situ hybridization method to detect bcr/abl fusion in cells with t(9;22)(q34;q11.2) associated with deletion of dna on the derivative chromosome 9 in chronic myelocytic leukemia.dual-color, dual-fusion fluorescence in situ hybridization (d-fish) can accurately detect and quantify cells with bcr/abl fusion in <1% of 500 nuclei in 80% of patients with chronic myelocytic leukemia (cml) and t(9;22)(q34;q11.2). the remaining patients have one of three forms of atypical d-fish patterns; these patterns have different sensitivities to detect disease. neoplastic cells with one abl, one bcr, and one bcr/abl fusion are particularly problematic, because normal cells with coincident ...200414697634
tetrahydrocortisol-apolipoprotein a-i complex specifically interacts with eukaryotic dna and gcc elements of genes.tetrahydrocortisol stimulates dna and protein biosynthesis in hepatocytes only when it enters the complex with apolipoprotein a-i. tetrahydrocortisol-apolipoprotein a-i (thc-apoa-i) complex specifically interacts with eukaryotic dna isolated from rat liver. in the process of interaction, rupture of hydrogen bonds between the pairs of nitrous bases occurs with the formation of single-stranded dna structures. in such state dna forms complexes with dna-dependent rna-polymerase. the most probable si ...200314698212
slc25a13-knockout mice harbor metabolic deficits but fail to display hallmarks of adult-onset type ii citrullinemia.adult-onset type ii citrullinemia (ctln2) is an autosomal recessive disease caused by mutations in slc25a13, the gene encoding the mitochondrial aspartate/glutamate carrier citrin. the absence of citrin leads to a liver-specific, quantitative decrease of argininosuccinate synthetase (ass), causing hyperammonemia and citrullinemia. to investigate the physiological role of citrin and the development of ctln2, an slc25a13-knockout (also known as ctrn-deficient) mouse model was created. the resultin ...200414701727
incidence and distribution of argininosuccinate synthetase deficiency in human cancers: a method for identifying cancers sensitive to arginine deprivation.argininosuccinate synthetase (ass) was the first of two enzymes to convert citrulline to arginine. this pathway allowed cells to synthesize arginine from citrulline, making this amino acid nonessential for the growth of most mammalian cells. previous studies demonstrated that several human tumor cell lines were auxotrophic for arginine due to an inability to express ass. selective elimination of arginine from the circulation of animals with these tumors is a potentially effective anticancer trea ...200414770441
[conservation and dilution of the horse and donkey; new dilutent with a milk base]. 195114896523
[experimental immunization of the mare against the donkey-antigen, and its development]. 195114926178
accessory spleens: preoperative diagnostics limitations and operational strategy in laparoscopic approach to splenectomy in idiopathic thrombocytopenic purpura patients.the preoperative detection of accessory spleen (as) is still a very important and serious problem. the aim of the study was to assess the reasons for failure and the long-term results of laparoscopic splenectomy (ls) in patients with idiopathic thrombocytopenic purpura (itp).200514968364
detection and nucleotide sequencing of a dna-packaging protein gene of equine gammaherpesviruses.in previous studies, novel putative viral pathogens designated that asinine herpesvirus 4 (ashv4) and asinine herpesvirus 5 (ashv5) were associated with fatal interstitial pneumonia in donkeys (equus asinus). nucleotide sequence analysis of a portion of the dna polymerase gene identified these putative pathogens as herpesviruses and possibly as members of the gammaherpesvirinae subfamily. although similar to equine herpesvirus 2 (ehv2) and equine herpesvirus 5 (ehv5), sequence diversity was obse ...200414974850
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