Publications

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correlation of the lymphocyte transformation, leucocyte migration inhibition and the delayed cutaneous hypersensitivity tests in chickens sensitised to mycobacterium tuberculosis. 1979120573
[effect of varying amounts of lysine in the ration on chickens].the effect of varying lysine quantities given in the daily ration to 20 day-old chickens was studied for a 40 day-period. highest body gain (157% as compared to the control group) was observed in group i (0.20% lysine). two and four per cent of lysine in the ration reduced the basic body gain by 69.7% and 66.9% respectively. the level of calcium, blood sugar, carotene and vitamin a rose considerably, while phosphorus and magnesium content was reduced. sgot and sgtp values remained without signif ...1979120632
the erythrocytic entry- and exit-mechanism of aegyptianella pullorum carpano, 1928.the erythrocytic entry- and exit-mechanisms of aegyptianella pullorum were investigated and characterized by scanning (sem) and transmission electron microscopy (tem) using for tem ruthenium red as a marker of the red cell plasmalemma. the scanned aegyptianella preparations produced static evidence of an endocytosis followed by an erythrocytic vesiculation as the possible mode of entrance of initial bodies into erythrocytes. the presence of ruthenium red only coating the membrane around the para ...1979120644
the influence of desoxycorticosteron on the thymus and bursa of fabricius in the chicken. 1979120663
[phospholipid and fatty acid composition of lamellar bodies isolated from chicken lungs at different stages of development].in order to elucidate whether the lamellar body content represents functional surfactant as soon as these bodies appear in lung epithelium during fetal development, lamellar bodies were isolated from lungs of chicken at various developmental stages. their content was analyzed and compared to extra-cellular functionnal surfactant. the results show that this content undergoes gradual changes in the phospholipid/protein ratio, as well as in the relative phosphatidylcholine amount. thus, in the chic ...1979120788
respiratory function of blood of the avians.individual blood samples were collected from wing veins of eight adult pigeons, two adult chickens and four adult geese and were analyzed for oxygen dissociation curves by hemo-scan, an instrument measuring po2 and o2 saturation simultaneously. the findings agree with the common belief that avian blood in general has a low affinity for oxygen. blood ph, pco2, concentration of 2,3-dpg, lactic acid, pyruvic acid, red blood cell, hemoglobin and hematocrit were also determined, the related parameter ...1978120802
adaptation of enzyme-linked immunosorbent assay to the avian system.a microplate enzyme-linked immunosorbent assay was developed to detect chicken anti-rovirus antibodies. studies of the parameters which affect the outcome of the assay with avian serum revealed two aspects for a successful assay. first, enzyme-antibody conjugates prepared by the periodate oxidation technique were found to have retained far more immunological activity than conjugates produced by a glutaraldehyde cross-linking. second, the results indicated an unusually high affinity of chicken im ...1979120876
evaluation of a killed rocky mountain spotted fever vaccine in cynomolgus monkeys.a nonhuman primate model of rocky mountain spotted fever infection was developed in cynomolgus monkeys (macaca fascicularis) infected by the subcutaneous route or by aerosol. clinical responses, hematology and serum chemistry values, and pathological findings were similar to those found in humans ill with rocky mountain spotted fever. the clinical model was then used to test the efficacy of a killed rocky mountain spotted fever vaccine grown in chicken embryo cells. monkeys were immunized with v ...1979120877
7-acetylcytochalasin b: differential effects on sugar transport and cell motility.cytochalasin b (cb) is a potent inhibitor of sugar transport and cell motility in animal cells. we have synthesized and characterized the cb derivative 7-acetylcytochalasin b (cbac) and have found that it has differential effects on transport and motile processes in fibroblasts. the derivative inhibited sugar transport in human red cells, 3t3 cells, and chicken embryo fibroblasts at micromolar concentrations, although it was less potent than its parent compound. unlike cb, which causes fibroblas ...1979120908
gravity, metabolic rate and body size of mammals. 1979120950
[pharmacokinetic and microbiological studies of pharmachem erythromycin asparate].anti-microbial activity and pharmokinetics of erythromycin aspartate "pharmachim" (ea) in chickens was studied. the investigations included erythromycin thiocyanat (et) of the company abbott-usa (the preparation gallimycin poultry formula). the studies revealed that ea has an effect on gram-positive microorganisms mainly. it is quickly resorbed by the digestive tract and following the administration of 50 and 100 mg/kg body weight (applied by tube) in doses of 0.14 to 0.8 meg/cm3 it can be detec ...1979120997
[stunning of animals for slaughter. part i. co2-stunning]. 1979121070
in vitro studies of rickettsia-host cell interactions: ultrastructural changes induced by rickettsia rickettsii infection of chicken embryo fibroblasts.secondary chicken embryo fibroblasts infected with the sheila smith strain of rickettsia rickettsii and grown in monolayer culture undergo rapid morphological alterations. transmission electron microscopic examination of cells at intervals after infection showed several progressive host cell lesions, including widespread dilatation of the rough endoplasmic reticulum and outer nuclear envelope and the accumulation of electron-dense material within the cisternae of intracellular membranes. dilatat ...1979121115
in vivo activation of murine peritoneal macrophages by nocardia water soluble mitogen (nwsm).in vitro nwsm has been shown to be a b cell mitogen and a polyclonal activator. although nwsm has been also shown to be adjuvant active in vivo, motogenicity and polyclonal activation are not observed when this fraction has been administered to mice. in contrast, the experiments reported here demonstrate that after in vivo administration several effects of nwsm on mouse peritoneal macrophages can be observed: nwsm was able to induce an important increase in the ability of peritoneal macrophages ...1979121200
effects of pesticides on the reticuloendothelial system.the information available concerning the effects of compounds utilized as pesticides on functions of the reticuloendothelial system is quite limited. review of selected examples of such studies in this and other reports (3) reveals considerable diversity in terms of species of experimental animal, purity and dose of pesticide, length of exposure, and class of pesticide employed. observations include depression, enhancement, or no significant effect on the selected reticuloendothelial system func ...1979121203
report prepared by the antibiotics in animal feddingstuffs sub-committee. microbiological assay of avoparcin in animal feeds and pre-mixes. 1979121209
[the metabolism of colostomised laying hens with 15n-labelled wheat. 4. 15n-incorporation in the corpuscular components of blood, in the blood plasma and the basic amino acids].three colostomised laying hybrids received over four days a dosage of 672 mg 15n-excess (15n'), 20.3 mg lysine 15n', 23.0 mg histidine-15n' and 66.7 mg arginine-15n' with a ration customary in production. after feeding the same nonlabelled ration for another four days the hens were killed and the n-content of the blood as well as of its fractions (corpuscular components, plasma, free amino acids of the plasma) was determined.1979121232
[in vivo occupation of estrogen receptors by hydroxylated metabolites of tamoxifen].we report that after in vivo administration of (3h) tamoxifen, the cytosol and nuclear estrogen receptor sites of rat uterus and chicken oviduct are mostly occupied by polar metabolites. one of the major metabolites is 4-hydroxy-tamoxifen which we have identified by cocrystallisation withe non radioactive compound and which is known to display a high affinity for the estrogen receptor. in the rat uterus, the proportion of the metabolites versus tamoxifen, increases with time with a maximum at 8 ...1979121266
physicochemical and comparative studies on bile proteins. 1979121295
[microbiological aspects of stored hard-boiled eggs].hard-bioled eggs in the shell were without any pretreatment stored in air at 20 degrees c and 4 degrees c. furthermore the keeping quality of varnish-coated eggs stored in air at 20 degrees c and of untreated eggs stored in 100% carbon dioxide at the same temperature was studied. hard-boiled eggs stored at 4 degrees c and varnish-coated eggs stored at 20 degrees c were of excellent bacteriological quality up to 5 weeks. untreated eggs stored in air at 20 degrees c showed high microbiological con ...1979121493
histopathological and lipid changes in various organs of chicks (gallus domesticus) caused by acute and chronic toxicity of metasytox-r and lebaycid. 1979121503
interaction of benzo[alpha]pyrene diol-epoxide with nuclei and isolated chromatin.chicken erythrocyte chromatin and nuclei were labeled with benzo[alpha]-pyrene (b[alpha]p) diol-epoxide (anti) and digested with micrococcal nuclease to mono- and dinucleosomes. analysis of the distribution of the carcinogen showed that the internucleosomal region bound 3-4 times more carcinogen per unit dna than did nucleosomes. the enhanced binding of the 'ultimate' carcinogen to the internucleosomal region was similar when isolated chromatin or nuclei were used for in vitro labeling. furtherm ...1979121556
[pure preparation of the fluorochrome acriflavine and its physico-chemical and cytochemical behavior]. 1979122374
relation between fluorescent intensity and extinction in quantitative microfluorometry. 1979122377
[determination of aflatoxins and toxicogenic fungi in mixed feed for poultry].the presence of aflatoxins was determined in 155 samples of chicken feeds, as well as their fungal contamination. aflatoxins were detected in 3 of the analized samples; one of them contained 1620 micrograms/kg, a highly dangerous amount for chickens. the amount found in the remaining 2 samples was 30 micrograms/kg. a study of toxigenic fungus showed a prevalence of 69.3% of aspergillus flovus: 72.4% of this percentage corresponded to aflatoxins producing strains. the prevalence of aspergillus oc ...1979122389
apolipoproteins and lipoprotein families in chicken embryos and egg yolk.1. turkey anti-lp-a and anti-lp-b cross react strongly with whole adult chicken serum. 2. similar reactions occur with whole chicken embryo sera (10-21 days of incubation) and egg yolk fluid. 3. ultracentrifugation of sera from 14-day old chicken embryos, after reaction with anti-lp-b, reveal complete precipitation of the vldl and ldl of embryo serum (indicating that adult-type lp-b is the major apolipoprotein of these density classes). 4. all of the chicken embryo adult-type apo-lp-a is in the ...1977122563
purification and partial characterization of high and low activity carbonic anhydrase isoenzymes from malaclemys terrapin centrata.1. high activity (ca c) and low activity (ca b) carbonic anhydrase isoenzymes have been purified from turtle erythrocytes. 2. the two isoenzymes differed in co2 hydration specific activity by 36-fold. 3. the low activity isoenzyme contained one half-cystine residue, whereas the high activity isoenzyme contained four half-cystines and required a reducing environment to maintain activity. both isoenzymes contained zinc. 4. molecular weights of 28,500 and 30,400 daltons were established for the low ...1979122575
penicillamine treatment of hereditary avian muscular dystrophy. 1977122684
distribution of neutral and acid alpha-glucosidases of granule fractions from chicken heterophil leucocytes. 1978122755
transplantation of lymphoid cells into immunodeficient chickens. dissociation in the reconstitution of b and t cell functions. 1975122790
in-vitro investigation of a new neuromuscular relaxant, ah8165.ah8165 was compared with other neuromuscular relaxants in an in-vitro rat phrenic nerve-diaphragm preparation. concentrations of 6-10 mug/ml ah8165 produced progressive decreases in strength of concentration. ah8165 was 0.1 times as potent as d-tubocurarine, and its effects were more rapidly reversed by washing. the times to recovery from 90 per cent blockade were the same for succinylcholine and ah8165, but the time to recovery from 50 per cent blockade was shorter for succinylcholine than for ...1975123133
[polychlorodibenzo-p-dioxins (author's transl)].polychlorodibenzo-p-dioxins (chlorodioxins) appear most frequently by heating of chlorophenols in alkaline solution specially when producing organic derivatives. consequently chlorodioxins may contaminate several industrial chemical products largely used, for instance, as pesticides. occasionally, chlorodioxins are also synthesized from chlorophenols or their derivatives in situ during industrial processes. chlorodioxins are the cause of chloracne and chick edema which killed thousands of broile ...1975123665
[the epidemiologic significance of tuberculosis mycobacteria of the avian type].reaction to intradermal injection of tuberculin from mycobacteria tuberculosis of the avian type proved to be negative in all the 505 students examined who never came in contact with fowl farms. of 738 local residents it was found to be positive in 23.8% and of 320 workers of fowl farms--in 54.5% of cases. in response to the injection of koch tuberculin and ppd-l the number of positive reactions in all the mentioned groups constituted 69-80%. a conclusion was drawn on the epidemiological danger ...1975123686
hybrid troponin reconstituted from vertebrate and arthropod subunits. 1975124018
[experiment on the isolation of tumor viruses from cell cultures of human neoplasms by inoculation into chicks (author's transl)]. 1975124216
ca-2+ transport and assembly of protein particles in sarcoplasmic membranes isolated from normal and dystrophic muscle. 1975124261
further miniaturization and automation of in vitro lymphocyte cultures.a microtechnique (10 mul culture volume) for in vitro lymphocyte cultures is described, which, compared with current miniaturized techniques, permits a four to 20-fold reduction of both medium volume and cell numbers. furthermore, two alternative procedures for harvesting and washing radioisotope labelled cells are described. the first, making use of a conventional harvesting device, collects cells on glass fibre filters in a two to three-fold shorter time than that needed for the current semi-a ...1975124480
on the specificity of the histochemical technique for sarcoplasmic reticular adenosine triphosphatase: a light and electron microscopic study.the specificity of the histochemical localization of the calcium activated adenosine triphosphatase (atpase) activity of the sarcoplasmic reticulum (sr) at ph 7.4 was studied using a calcium-citro-phosphate technique. the latter involves the splitting of atp by atpase producing phosphate ions which then react with calcium and citrate to form an insoluble reaction product. this reaction product was detected by both light and electron microscopy. light microscopic examination showed a darkly stain ...1975124715
[the progenesis of the trematode prosotocus confusus (loss, 1894)--an amphibian parasite]. 1975124842
[effectiveness of spectam in chicken coliform septicemia]. 1975124991
erythrocyte phosphofructokinase deficiency associated with an unstable variant of muscle phosphofructokinase.a case of chronic non-spherocytic hemolytic anemia due to partial erythrocyte phosphofructokinase deficiency (61% of normal) is reported. immunological studies in hemolystates, using anti-muscle and anti-leukocyte phosphofructokinase antisera, seemed to indicate that an isozyme of the muscle type was deficient in the patient. this hypothesis was confirmed by the studies of muscle phosphofructokinase; this enzyme was an unstable and fast variant. there was no deficiency in muscle because of the a ...1975125160
interrelationship of dietary silver with copper in the chick.adding 900 p.p.m. silver (as silver nitrate) to a practical diet for chicks significantly depressed growth, increased wet and dry heart weight to body weight ratios and markedly increased mortality during a four-week experimental period. blood packed cell volume was not affected. supplementing the diet containing silver with 50 p.p.m. copper prevented cardiac enlargement and mortality, but only partially corrected the growth depression. glycogen content of the heart was not affected, but aortic ...1975125418
studies on the fragmented sarcoplasmic reticulum and "natural" tropomyosin of normal and dystrophic chickens. 1975125420
possible serotonergic mediation of tonic immobility: effects of morphine and serotonin blockade.small doses of morphine, within the range of 0.02 mg/kg to 10.15 mg/kg were administered to chickens (gallus gallus) and the effect on the fear-based tonic immobility (ti) response determined. all doses at and above 0.13 mg/kg enhanced the ti response significantly above the levels produced for the 0.2 mg/kg dose and the distilled water control group. administration of a single large dose of p-chlorophenylalanine prevented the morphine-induced enhancement of ti. it was suggested that serotonin s ...1975125432
[sodium reabsorption and the membrane systems of vertebrate kidneys].sodium reabsorption is low in coldblooded animals (the lamprey lampetra fluviatilis, red salmon oncorhynchus nerka, carp cyprinus carpio, frog rana temporaria, tortoise agryonemis horsfieldi) being much higher in warmblooded ones (chicken, pigeon, rat). high level of na transport in warmblooded animals is paralleled by high activity of succinate dehydrogenase as compared with the activity of na,k-atpase. the content of monoenoic fatty acids in the glycerophosphatides from frog and tortoise kidne ...1975125513
pathogenesis of acute avian malaria. iii. antigen and antibody complexes as a mediator of anemia in acute plasmodium gallinaceum infections of chickens.in a study of antigens and antibodies found in malarious chicken blood, antigen activity was found in plasma, and in hypertonic saline eluates of blood cells. a soluble antigen was extracted from parasites liberated from erythrocytes. two classes of antigen were differentiated, one a globulin associated "serum antigen" which was found to show identity with a serum antigen from blood of rats with acute babesia rodhaini infection, and another that was associated with the plasmodium gallinaceum par ...1975125550
comparative studies on 5,5'-dithiobis-(2-nitrobenzioc acid)-treated myosins.myosins prepared from chicken and rabbit fast and slow muscles were treated with 5,5'-dithiobis-(2-nitrobenzoic acid) (nbs2). about half of the thiol groups of the fast muslce myosins reacted with nbs 2, but in slow muscle myosins, only about 10-20% of the thiol groups reacted. this treatment removed 50-60% of the l2 components, nbs2 light chain, from fast muscle myosins, but did not result in specific dissociation of the light chains in slow myscle myosins. the treatment sometimes released l4 c ...1975125749
potassium transport by the isolated perfused kidney.rat kidneys perfused outside of the body with an artificial medium are able to increase their fractional excretion of potassium in response to a rising concentration of potassium in the medium but never show net secretion of potassium. by contrast, isolated perfused kidneys from chronically potassium-loaded rats regularly secrete potassium in excess of the amount filtered. ouabain completely blocks the secretion of potassium by these isolated kidneys, suggesting that na-k-atpase mediates potassi ...1975125766
plaque development by vaccinia-viruses on the chicken chorio-allantois under the influence of cyclophosphamide.the development of vaccinia-virus plaques on the chorioallantois of embryonated eggs was studied under the influence of active cyclophosphamide metabolites. the viruses were inoculated on the 9th or the 13th incubation day. cyclophosphamide was injected in doses of 62.5--500 mug in 0.2 ml nacl into the yolk sac either 7 hrs after the virus inoculation or 3, 6, or 9 hrs in advance. the plaque development was not influenced when cyclophosphamide was given after the virus inoculation. but in treati ...1975125958
differential response in cardiomyopathy of chicks and turkeys to furazolidone toxicity.dietary furazolidone (500 ppm or higher) significantly slowed the growth of chicks fed either a conventional or a purified diet and of turkeys fed a conventional diet. deaths were significantly increased in turkeys fed 500 ppm and in chicks fed 700 ppm in the purified diet. ratio of heart to body weight was significantly increased by furazolidone in turkeys, but not in chicks. furazolidone did not induce anemia in either species. turkeys and chicks differed markedly in cardiac response to toxic ...1975126057
extent and topography of the acetylation of calcified chicken-bone collagen.acetic anhydride in anhydrous acetone-triethylamine reacts with decalcified bone collagen in two ways. it completely acetylates the e-amino groups of lysine and hydroxylysine and causes a progressive change in the protein matrix, such that increasing amounts of degraded peptides become soluble in alkaline phosphate solution. the peptides produced have molecular weights ranging from 100,000 to less than 2,000 daltons. the number of peptides obtained indicates that there are about 10 bonds cleaved ...1975126138
regulation of muscle contraction: bindings of troponin and its components to actin and tropomyosin.the bindings of troponin components to actin and tropomyosin has been studied by cosedimentation with actin and affinity chromatography. it is shown that troponin binds to actin and tropomyosin in the presence and absence of calcium but the binding to actin is sensitive to ionic strength. troponin-i + c binds to actin-tropomyosin in the absence of calcium but not to actin or tropomyosin alone. troponin-i binds to actin and the binding is improved in the presence of tropomyosin even though tropon ...1975126151
preparation and properties of vertebrate smooth-muscle myofibrils and actomyosin.a new technique for obtaining a myofibril-like preparation from vertebrate smooth muscle has been developed. an actomyosin can be readily extracted from these myofibrils at low ionic strength and in yields 20 times as high as previously reported. the protein composition of all preparations has been monitored using dodecylsulfate-gel electrophoresis. by this method smooth muscle actomyosin showed primarily only the major proteins, myosin, actin and tropomyosin, while the myofibrils contained, add ...1975126155
a radioisotopic method to measure delayed type hypersensitivity in the mouse. ii. cell transfer studies.sensitized lymphoid cells could transfer to normal non-sensitized (naive) mice by 24-48 h after antigen challenge in the ear, the capacity to incorporate, at the site of antigen deposition, 5-iodo-2'deoxyuridine-125i in amounts significantly above those obtained in control mice. this was associated with a mononuclear cell infiltration in the pinna. in contrast to lymphoid cells, serum antibodies were unable to transfer a 24- or 48 hour ear reaction. the cells responsible were t lymphocytes as de ...1975126212
design and fabrication of two types of artificial tendons for evaluation of surgical techniques.double-loop tendons of fixed and adjustable lengths, and tendons with inner porous tapes both for anastomosis to tendon stumps have been made from readily available materials. by using stainless steel wire and polymer fibers, breakloads have been obtained that range from 20 to 100 lb and elongations have been reduced to less than 2%. pore sizes up to 50 times 250 mu2 were achieved by using woven, knitted, and braided polyester tapes. for all tendons, silastic was used to isolate inner fibers and ...1975126245
membrane transport during development in animals.this brief and necessarily incomplete survey of available evidence on the development of transport systems in animal cells reveals a primitive state of knowledge full of interesting possibilities for future development. the assembly of membrane-bound transport systems during embryonic development provides unique opportunities for approaching questions relating to gene expression, the synthesis and insertion of membrane proteins into phospholipid layers, the composition and structure of transport ...1975126702
[ultrastructural histogenesis of chicken myocardium].the structure of the ventricular myocardium of the chick embryo was studied from the 4th day of incubation until one day after hatching. special attention was paid to the differenciation of myofibrils, their branching at the level of stria z and their relations with desmosomes.1975126792
the onset and progress of transformation of avian slow into fast muscles under neural influence.the slow anterior latissimus dorsi (ald) muscles of newly hatched chickens were transposed and cross0innervated by the mixed, predominantly fast superior brachialis nerve, and investigated 2 to 15 months after the operation. two months after the operation, myosin atpase activity of the cross-innervated ald muscles was still as low as in the control ald, although the ultrastructure and the histochemical atpase activity already showed a mixed fibre-type pattern with a predominance of fast -type fi ...1975127164
involvement of biogenic amines in drug-induced aggressive pecking in chicks.pairs of neonate chicks were administered psychoactive agents and pecks against each other were recorded during 4-hr test sessions. the first experiment assessed where drug-induced aggressive pecking could serve as a useful anti-depressant screening model. although pecking was induced by tricyclics, d-amphetamine, and l-dopa, ineffective agents included a mao inhibitor (pargline) and a tricyclic indole antidepressant (iprindole). these data cast doubt on the validity of the chick pecking model a ...1975127189
[pseudomonas aeruginosa--the causative agent of infection in birds].pseudomonas aeruginosa was isolated from the viscera of diseased and dead birds (young and adult chickens) as well as from 9 poultry houses on seven farms. its typing was performed on the basis of the blue-green coloration of the cultures, their hemolytic activity, the pleasant odour of lime-tree, the growth in culturing at 42 degrees c, the presence of the pyocianin pigment, and the positive results of the cytochromoxidase test after gaby and hadley and the biochemical test after kovacs. the pa ...1975127412
heteroagglutinins and spontaneous rosette-forming cells in obese strain (os) chickens.sera of os chickens with spontaneous autoimmune thyroiditis (sat) and of normal white leghorn (nwl) controls, aged 4-20 weeks, were studied for the presence of heteroagglutinins to sheep (srbc) and rabbit red blood cells (rrbc). significantly higher frequency and titers of srbc- and rrbc-agglutinins were found in os as compared to nwl birds, the difference being more accentuated with rrbc. the number of spontaneously rosette-forming peripheral blood lymphocytes (rfc) with srbc and rrbc was deter ...1975127457
cytological changes and antigen capture in chicken spleen during the secondary immune response to homologous and cross-reacting antigens.it was demonstrated by autoradiography using 125i-labeled antigens that in the secondary response to bovine serum albumin (bsa), a considerable amount of antigen is localizing in the germinal centers (gc) of chicken spleen prior to the onset of antibody production. a similar early antigen capture, although of a lesser degree, was also noted when birds originally immunized with bsa were injected with human serum albumin (hsa) on a second occasion. early antigen localization in gcs was scarcely de ...1975127461
[pathogenesis of neural lesions in marek's disease. i. allergic skin reaction against myelin of the peripheral nerves (author's transl)].23 chickens affected with marek's disease were tested by the allergic skin reaction for delayed hypersensitivity against myelin and myelin preparations of peripheral nerves. 19 animals proofed to be positive in the skin test, yet there was no correlation with the intensity of the nerve affections. 4 animals with severe mononuclear neural infiltrations didn't show any allergic skin reaction. it could be demonstrated that in marek's disease lymphocytes are sensitized against components of the peri ...1975127473
the immunological specificity of myosins from cross-striated muscles as revealed by quantitative microcomplement fixation and enzyme inhibition by antisera.the immunological properties of myosins, especially their muscle-type, class and species specificity, are still controversial. it is the opinion of the authors that the lack of agreement might at least in part be due to the use of contaminated myosins as immunogens and inappropriate methods. we, therefore, purified myosins to a very high degree (approximately 99%) and induced antisera in guinea-pigs. studies of quantitative microcomplement fixation and enzyme-inhibition by antisera yielded the f ...1975127617
using the hml1 strain of histomonas meleagridis in evaluating chemotherapeutants in chicks.ten-day-old white leghorn cockerels were inoculated per cloaca with 500,000 culture forms of the hml1 strain of histomonas meleagridis. birds dying on the 6th and 7th days and birds necropsied 7 days postinoculation had prominent cecal and liver lesions indicative of severe histomoniasis. dose responses were obtained with three 1,2-disubstituted 5-nitroimidazoles blended into the feed at various concentrations, demonstrating the utility of this virulent strain in chemotherapy studies.1975128345
purification and structural analysis of myosins from brain and other non-muscle tissues. 1975128633
enzymatic basic for dde-induced eggshell thinning in a sensitive bird. 1976128701
[control of the dust content of the air in poultry houses]. 1975128879
[epizootiology of the acute form of marek's disease]. 1975128883
[influence of an antiandrogen, cyproteron acetate, on the morphogenesis of the epididymis in the chick].morphogenesis of the epididymis, which takes place in the chick, during the two months after hatching, is inhibited by the antiandrogenic drug, cyproteron acetate, which acts as strongly as castration. this fact is a good argument for our conception of sexual differentiation after which epididymis is induced by testosterone.1975129199
[early teratogen effect of neutral desoxyribonuclase on young chick embryo].neutral dna-ase acts as a general teratogen when given to young chicken blastoderms. the rate of the differents abnormalities varies according to the dose given and the embryos' stage. the frequency of the different defects reaches a maximum value with increasing doses it falls generally down with more important doses. dmso added to the enzyme solution changes the susceptibility of the embryos.1975129211
[adsorption of lymphocytes by cells infected by vaccinia virus].the interaction of chicken lymphocytes with vaccinia-infected cells was examined hep-2 cells infected with the virus adsorbed chicken lymphocytes. the phenomenon was inhibited by anti-vaccinia serum. lymphocytes prepared from a chicken possessing red blood cells insensible to the hemagglutinin of vaccinia virus were also adsorbed. lymphocytes were adsorbed in similar degree at 37 degrees c and at room temperature.1975129247
[on the incidence of mite eggs in human stools (author's transl)]. 1975129296
the effect of endotoxin and human dental plaque on the respiratory rate of bone cells. 1975129554
[veterinary and sanitary measures and the prevention of poultry diseases]. 1975129919
[natural foci of ornithosis in kazakhstan].an investigation (clinical, immunological and epidemiological) of the workers of various fowl-farms was carried out in various regions of kazakhstan for a number for years; the sera of domestic fowl and birds was studied as well. on the basis of examination of 6264 persons the greatest percentage of ornithosis was revealed among the workers of the slaughter house and the shop for the processing of the fowl. examination of 4749 sera of bird blood showed that the highest incidence of ornithosis wa ...1975130041
immunological comparison of normal, regenerating, and cultured corneal cells.two types of corneal cell membrane receptors are described, one occurs on the surface of only regenerating or cultured cells, that is, actively metabolizing and/or proliferating ones. the other type of corneal membrane receptors occurs on the cell surface independent of the metabolic state, e.g. on excised resting cells. in contrast to the restricted ontogenic specificity of the metabolically dependent corneal cell membrane antigens, the metabolically independent cell surface antigens were found ...1975130113
variation of oviductal luman atpase with the ovulatory cycle of the hen. 1976130171
fine structure of the avian muscle spindle capsule.the capsule of the muscle spindle from the anterior and posterior latissimus dorsi muscles of the adult domestic chicken has been studied with the electron microscope. as in other species, two distinct portions of the spindle capsule are distinguished: an outer capsule and an inner capsule. the outer capsule is structurally similar to and continuous with the perineural epithelium. outer capsule cells are noted by the abundance of pinocytotic vesicles and a network of 6-7-nm microfilaments. the d ...1976130210
natural anti-ovalbumin agglutinins in adult chickens.sera from inbred spf white leghorn chickens at 6 weeks or 19-22 months of age did not have anti-oa agglutinins as determined by the passive ha reaction; 2-day-old chicks also lacked the anti-oa activity. inbred chickens kept in spf isolators for 6 weeks, then transferred to a conventional environment for 7 weeks, produced anti-oa agglutinins. inbred white leghorn adult chickens conventionally reared had a high anti-oa agglutinin titer. it appeared that natural anti-oa agglutinin s are elaborated ...1976130635
cytochemical studies on sarcolemma: na+, k+-adenosine triphosphatase and adenylate cyclase.two highly lead-sensitive atpases, na+,k+-atpase and adenylate cyclase, can be demonstrated cytochemically by the lead precipitation technique in briefly prefixed tissue, provided that the free pb2+ concentration in the incubation medium is kept below 0.1 mm by a heavy metal chelator. under conditions suitable for na+,k+-atpase activity precipitation of final reaction product (lead phosphate) at the sarcolemma of cardiac muscle is abolished by 0.1-1mm ouabain. in contrast, reaction product depos ...1976130656
a relationship between ca2+ sensitivity and phosphorylation of gizzard actomyosin. 1976130907
a comparative study of skeletal and cardiac tropomyosins: subunits, thiol group content and biological activities.the subunit composition, the thiol group content and the biological activities of cardiac tropomyosins (tm) of various animal species were compared. cardiac tm from small animals such as rabbit, guinea-pig, rat and dog contain 2 sh/mole and were resolved into one band on sds and acid urea electrophoresis and into two bands on alkaline urea electrophoresis. chicken cardiac tm likewise gave one band and it contains 4 sh/mole. in contrast pig, sheep and human cardiac tm contain respectively 2.6, 2. ...1976131298
[development of germ cells in chick ovarian medulla].ovaries from chick embryos and chicken have been investigated with a view to the evolution of germ cells in the medullary. they can enter meiosis and reach pachytene. they seem to be eliminated by the way of the lacunas. some observations can be utilized in a discussion about the initiation of the meiosis and the evolution of the ovocytes.1975131621
[sleen enhancement reactions in chick embryo after spleen homografting: autohistoradiography after labeling of graft with tritiated thymidine].the use as a graft involving an embryonic spleen enhancement of the spleen of an embryo previously grafted (with adult spleen) allowed a very high labelling (3h-thymidine) of the graft and therefore the study of ingrafted cells behaviour: a) on the chorioallantois membrane, b) on the host spleen. in the last case, only a very little number of labelled cells was observed and the whole results confirm the hypothesis of an active participation of the embryo in the case of such an haemopoietic splee ...1975131624
[spleen enhancement reactions in chick embryo after homografting adult chicken spleen fragments: changes in spleen cell population in relation to rate of spleen enhancement].a method using the comparison between the modifications of spleen weight and on spleen smears visible modifications of the distribution into categories of cells which are classified by the mean of their maturation degree, suggested that these both types of modifications are bound. therefore, the classic cytological study seems to be a really good tool for analysing the effects of this kind of grafting on the haemopoietic organs, the weight modifications of which are difficult to measure. on the ...1975131631
characterization of a t-lymphocyte inhibitor in the serum of tumour-bearing mice.sera from mice with large tumours from a variety of tissue types and sources have been shown to contain substances capable of suppressing the proliferative response of normal mouse lymphocytes to concanavalin a (con a), bacterial endotoxin (lps) and allogeneic cells. the present paper deals with studies on the nature of these inhibitory materials using mainly a methylcholanthrene-induced rhabdomyosarcoma in dba/2j mice. it was found that a material responsible for inhibition of the con a respons ...1976131782
myosin-linked calcium regulation in vertebrate smooth muscle. 1976131863
[100,000,000 eggs per year]. 1976132002
hypothermic effect of cocaine in rats.cocaine injected intraperitoneally into rats resulted in a dose-dependent hypothermia. intracerebral injection of smaller doses also produced a fall in body temperature. in rabbits and guinea-pigs, cocaine produced hyperthermia, in mice and chicks it produced hypothermia while inconsistent changes were produced in goats. pre-treatment of rats with 6-hydroxydopamine, alpha-methyl-m-tyrosine or haloperidol significantly antagonized the cocaine hypothermia. pre-treatment of the rats with either hyo ...1975132013
isolation and properties of platelet myosin light chain kinase.a protein kinase which phosphorylates the 20 000-dalton light chain of platelet myosin has been isolated from human blood platelets and purified approximately 600-fold. elution of a 7.5% polyacrylamide gel following electrophoresis of the partially purified enzyme yielded a single peak of kinase activity which could be aligned with a protein band on a stained gel. assuming a globular shape, a native molecular weight of 83 000 (+/- 10%) was determined by gel filtration on bio-gel p-200. the kina ...1976132187
isolation and partial characterization of chick brain synaptic plasma membranes.an isolation procedure for synaptic plasma membranes from whole chick brain is reported that uses the combined flotation-sedimentation density gradient centrifugation procedure described by jones and matus (jones, d. h. and matus, a. i. (1974) biochim. biophys. acta 356, 276-287) for rat brain. the particulate of the osmotically shocked and sonicated crude mitochondrial fraction was used for a flotation-sedimentation gradient step. four fractions were recovered from the gradient after 30 min cen ...1976132194
[incidence of early excision of the retina on the development of the optic pedicle in the chicken].the retina cutting of 4 days old chick embryos determine the disappearing of the pedicle optic cells. absence of the optic fibers result in a absence of the glial cells differentiation.1975132253
[an attempt to cultivate the agent connected with the antigen of hepatitis b in chicken embryos (author's transl)]. 1976132298
[pharmacological characteristics of a new curariform compound from a series of naphthalene-peri-tetracarboxylic acid derivatives].experiments staged with rabbits, cats, frogs and chicken have proved tetracarboxylic acid dibenzosulphoethylate-bis (para-piperidinophenol)-diimidonaphthalene-1, 4, 5, (see article) to be capable of totally blocking the neuro-muscular conduction in a dose of 0.5 mg/kg and to block 20 per cent of it in a dose of 0.3 mg/kg. the action of the compound on the arterial pressure is half as great as that of d-tubocurarine. a pharmacological analysis conducted on the musculus rectus abdominus of the fro ...1975132362
functional and structural roles of sarcoplasmic reticulum protein components. 1975132367
effects of long-term increased gravitational load on intrafusal fibers on the avian muscle spindle. 1976132986
syngamus trachea: infections produced by prenatal inoculations of chicken embryos. 1976133028
characterization of the active site of platelet myosin in comparison to smooth and skeletal muscle myosin. 1976133634
the role of matrix vesicles in calcification. 1976133751
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