| the reaction of mouse muscle cells during simultaneous trichinella spiralis and trichinella pseudospiralis infection. | | 1980 | 7246268 |
| recovery of eosinophils from the peritoneal cavity of the guinea pig. | we studied the effects of various conditions on the recovery of eosinophils from the peritoneal cavity of guinea pigs repeatedly lavaged with saline. we compared the effects of ether and halothane on eosinophil production in guinea pigs either lavaged with saline alone or receiving an injection of polymyxin b before saline lavage. with both anesthetics polymyxin b caused a rapid and consistent increase in eosinophil production, although neutrophils were present. with halothane anesthesia, saline ... | 1981 | 7264308 |
| the effects of host sex and hormones on trichinella spiralis in the mouse. | the effects of host's sex and hormones on the number of adult trichinella spiralis in the small intestine, the number of migratory larvae produced in vitro by adult female worms, and the number of muscle larvae per gram of body weight were examined in cd-1 swiss white mice. nongonadectomized (intact) male mice housed greater numbers of adult worms and a greater number of muscle larvae per gram of body weight than did intact female mice. adult female worms isolated from intact male mice deposited ... | 1981 | 7264840 |
| lymphocytotoxicity of mouse sera in the course of experimental infection with trichinella spiralis and trichinella pseudospiralis. | | 1980 | 7272822 |
| reactivity of lymphocytes in germfree and conventional mice infected with trichinella spiralis. | | 1980 | 7272823 |
| scanning electron microscope studies in the small intestine epithelium of germfree and conventional mice after infection with trichinella spiralis. | | 1980 | 7272829 |
| macrophage migration inhibition test in germfree and conventional mice infected with trichinella spiralis larvae. | | 1980 | 7272830 |
| haematological changes evoked by trichinella spiralis as an index of the course of trichinellosis in germfree and conventional mice. | | 1980 | 7272831 |
| immunopathological changes in the kidneys of mice experimentally infected with trichinella spiralis. | | 1981 | 7299149 |
| comparative analysis of mobility and ultrastructure of intramuscular larvae of trichinella spiralis and trichinella pseudospiralis. | the muscle phase of trichinella spiralis and trichinella pseudospiralis was studied by using scanning electron microscope techniques, closed circuit television, and video tape recording. the complete absence of any cyst structure including the pseudocapsule allows t. pseudospiralis to move freely between the muscle layers. its rate of activity, measured as distance moved between two points, was 2.83 mm/min compared to that of the encysted t. spiralis larvae which was 0.237 mm/min. there was an a ... | 1981 | 7299578 |
| chemical attraction in the absence of worm-mediated tactile behavior in trichinella spiralis. | in vitro chemical attraction of trichinella spiralis was studied using as migrators individual male or female worms. both male and female worms exhibited a dose-dependent behavior at target doses of 20 to 80 worms, no significant differences in response at doses of 80 to 200 worms, and an inhibition of movement at doses of 200 to 400 worms. single males were attracted less to a mixture of males and females as the source of pheromone than single males to a female source. single females did not mo ... | 1981 | 7299579 |
| partial purification and characterization of protection-inducing antigens from the muscle larva of trichinella spiralis by molecular sizing chromatography and preparative flatbed isoelectric focusing. | the soluble portion of a large particle fraction which was derived from the muscle larva of t. spiralis was subjected to molecular sizing column chromatography using sephacryl s-200. five major peaks of 280 nm absorbing material were obtained. analysis by immunoelectrophoresis revealed that each peak contained antigens, with the majority of them occurring in peaks 3, 4 and 5. preliminary studies indicated that peak 4(mol. wt range 20 000--10 000) contained protection-inducing antigens. crossed-i ... | 1981 | 7301410 |
| the effect of trichinella spiralis on the rate of clearance of 51cr-labelled sheep red blood cells and 125i-polyvinyl pyrrolidone from the bloodstream of mice. | mice infected for one to eight weeks with trichinella spiralis cleared either 51cr-labelled sheep red blood cells (srbc) or 125i-polyvinyl pyrrolidone (pvp) given intravenously. pvp was cleared more rapidly from the blood of mice infected for one week than from uninfected mice with older infections which behaved in a similar manner to the controls. srbc were consistently cleared faster from the blood of mice infected for one week than from normal mice. if the infections were older than this a mo ... | 1981 | 7310111 |
| characterization of surface antigens of trichinella spiralis infective larvae. | infective larvae of trichinella spiralis were surface-labelled with radioactive iodine, and the products were characterized biochemically and immunochemically. the labelled material was restricted to two basic subunits: a lentil lectin-adherent glycoprotein (gp), mol. wt 47k, and a lentil lectin-nonadherent protein fraction (p), mol. wt 55k. both of these form homologous dimers through as yet unspecified covalent bonds to yield gp90 and p105. gp is further polymerized into higher molecular weigh ... | 1981 | 7322614 |
| trichinella spiralis in rats: in vivo effects of the bile and in vitro action of secretory iga from bile. | the biliary secretion of rats is diverted from the choledoch duct to the bladder. these rats are resuscitated by sub-cutaneous injections of a salt solution and then infected with trichinella spiralis larvae. it is shown that the number of adult worms is increased (+ 66%); as is the female larvae production (+ 51%); the females' length is also increased (+ 25%). 40 days after the infection the number of muscular larvae is considerably increased (+ 79%) in comparison with control rats. secretory ... | 1981 | 7325526 |
| gut propulsion in mice infected with trichinella spiralis. | previous investigators have shown that trichinella spiralis increases intestinal motility and propulsion. we report here that primary infection with t. spiralis in the mouse increased gut propulsion, measured by the movement of nonabsorbable chromatography beads, on day 5 after infection but not 9 days after infection. both cortisone acetate, an anti-inflammatory agent, and lomotil, which reduced gut motility, could suppress the increase in gut propulsion seen 5 days after infection. the results ... | 1981 | 7328463 |
| a comparative study of trichinella spiralis (owen, 1835) and trichinella pseudospiralis (garkavi, 1972). | | 1981 | 7328464 |
| the effects of host sex on enteric response to infection with trichinella spiralis. | the effects of host sex on enteric inflammatory response to primary infection with trichinella spiralis were examined. enteritis in female mice was greater than that in male mice on days 7, 9 and 11 postinfection (pi) and similar to that in male mice by day 13 pi. male and female mice given daily injections of 33 or 66 mg/kilo of cortisone acetate exhibited significantly reduced enteritis on days 7, 9 and 11 pi below that seen in uninjected, male and female mice. on days 7, 9 and 11 pi larviposi ... | 1981 | 7328465 |
| [growth and fertility of trichinella spiralis developed from larvae treated with noviben and panacur in vivo]. | | 1981 | 7336738 |
| evidence, nature, and implications of the constitutive resistance to trichinella spiralis in gallinaceous birds. | white leghorn chickens and mice were orally infected with trichinella spiralis larvae and their digestive tracts were examined for parasites at postinfection hours (pih) 5 and 10, and at postinfection days (pid) 1, 2, 4, 6, and 8. evacuated feces were examined at pih 5 and 10 and at pid 1, and blood was examined at pid 4, 6, and 8. with the exception of trichinae in the digestive tract (9 at pih 5 and 2 at pih 10), parasites were not recovered from chickens. all mice had abundant parasites in th ... | 1981 | 7337291 |
| intestinal mast cell response in thymectomised and normal mice infected with trichinella spiralis. | in nih mice, expulsion of trichinella spiralis from the small intestine and increase in intestinal mast cells were each dependent on the presence of t-lymphocytes. both changes were deficient in thymectomised mice but could be largely restored by reconstitution of thymectomised mice with syngenetic mesenteric lymph node cells. in both nih and balb/c mice the majority of the increased number of mast cells occurred within the intestinal epithelium. in nih mice increase in the number of intestinal ... | 1981 | 7344269 |
| host defense mechanisms against trichinella spiralis infection in the mouse: eosinophil-mediated destruction of newborn larvae in vitro. | | 1980 | 7350232 |
| mebendazole therapy of parenteral trichinellosis. | mebendazole was highly effective against the helminth parasite trichinella spiralis in mice subjected to a 3-day course of treatment during the invasive and encystment phases of experimental trichinellosis. when treatment began either 2 or 4 weeks after the mice were inoculated with parasites, the number of larvae developing in the host musculature was greatly reduced by twice-daily oral administration of 3.125, 6.25, or 12.5 milligrams of mebendazole per kilogram of body weight. | 1980 | 7355285 |
| survival of trichinella spiralis larvae in deep-frozen wolf tissue. | | 1980 | 7363256 |
| trichinella spiralis: comparison with an arctic isolate. | | 1980 | 7364011 |
| surface morphology of trichinella spiralis by scanning electron microscopy. | the surface morphology of larval and adult trichinella spiralis was studied by scanning electron microsocopy (sem) of fixed, dried, and metal-coated specimens. the results are compared with those found earlier by various investigators using light and transmission electron microscopy. some morphological features reported here are revealed uniquely by sem. these include the pores of the cephalic sense organs, the character of secondary cuticular folds, variations of the hypodermal gland cell openi ... | 1980 | 7365639 |
| chemical attraction in the genus trichinella. | chemical attraction in the genus trichinella was studied in vitro. male adult worms of trichinella spiralis, t. spiralis var. pseudospiralis, and trichinella sp. isolate (polar bear isolate) were attracted to females of their own isolates. the intensity of heterosexual chemical attraction between t. spiralis and the trichinella sp. isolate was similar, whereas t. spiralis var. pseudospiralis males were attracted less by either t. spiralis or the trichinella sp. isolate females. female worms of a ... | 1980 | 7365640 |
| studies on in vitro larvaposition by adult trichinella spiralis. | | 1980 | 7365641 |
| trichinella spiralis infection in animals genetically selected for high and low antibody production. studies on intestinal pathology. | specific antibody response and histopathological changes in the small intestine were studied in mice genetically selected for high and low antibody production (hl and ll, respectively) after oral infection with 50 muscle larvae of trichinella spiralis. specific antibody response, measured during the first 20 days after infection in the passive cutaneous anaphylaxis assay and the immunofluorescence test was high in hl and low in ll mice. the response of macrophages, intestinal mast cells, globule ... | 1980 | 7372360 |
| immunity to trichinella spiralis in irradiated mice. | | 1980 | 7372390 |
| trichinella thrives in both oxidative and glycolytic human muscle fibres. | in a deltoid biopsy from a severe case of human trichinosis the larvae of trichinella spiralis did not exhibit any predilection for any particular fibre type. type i and type ii fibres were involved with a frequency proportionate to their distribution in the muscle sampled. | 1980 | 7373326 |
| [evaluation of helminth intradermal tests in comparison to coproovoscopic surveys]. | 1600 subjects connected with mining were divided into 6 groups and examined because of intestinal parasites. there were: surface miners underground miners, retired miners and two groups of school-children up to 14 years old and from 14-17 years old, and persons not connected with the miners' work. the one control group consisted of 150 persons with negative coproscopical investigations, the other consisted of 112 persons, who were clinically healthy. the coproscopical investigations, morphologic ... | 1980 | 7377624 |
| hypereosinophilia in rats with trichinella spiralis infections. | | 1980 | 7378271 |
| effects of the host immune response on the longevity, fecundity and position in the intestine of trichinella spiralis in mice. | in female nih strain mice, expulsion of a primary infection of the nematode trichinella spiralis began on day 8 and was virtually complete by day 14 of infection. in secondary and tertiary infections, the number of larvae which established in the intestine was normal, but expulsion began on day 6 and was complete on day 10. in a primary infection the shedding of larvae by female worms began on day 5, reached its peak on days 6--7, began to decrease on day 8 and was minimal by day 10. in secondar ... | 1980 | 7383709 |
| immunologically mediated, non-specific interactions between the intestinal phases of trichinella spiralis and nippostrongylus brasiliensis in the mouse. | interactions between infections of trichinella spiralis and nippostrongylus brasiliensis were studied in the nih strain of mouse which is known to react strongly to t. spiralis. the course of n. brasiliensis infection in this strain of mouse is described and expulsion is shown to be accelerated in immunized mice and inhibited in cortisone-treated mice. there was no evidence of inter-specific competition between the two species of worm in concurrent infections; the number and location of adults o ... | 1980 | 7383710 |
| phospholipase b levels in fecal pellets from mice infected with trichinella spiralis, hymenolepis nana, and schistosoma mansoni. | fecal pellets of mice infected with trichinella spiralis, hymenolepis nana, and schistosoma mansoni have been found to contain high levels of phospholipase b activity. the rise, time course and decline of the enzymatic content of the pellets correlate with the known patterns of intestinal injury and reaction due to the parasites or their eggs. treatment with drugs (thiabendazole, niclosamide, niridazole) which are effective in suppressing the infection also prevents the rise, or causes an early ... | 1980 | 7386719 |
| eimeria nieschulzi and trichinella spiralis: analysis of concurrent infection in the rat. | | 1980 | 7389855 |
| systemic anti-inflammatory effect associated with enteric trichinellosis in the rat. | the hypothesis tested was that enteric parasites that induce local inflammation in their host suppress inflammatory reactions at distant sites. a technique was developed to demonstrate, quantitatively, such an anti-inflammatory component. the systemic antiphlogistic effect produced by the intestinal stages of trichinella spiralis was studied and compared with that caused by dexamethasone, a synthetic adrenocortical steroid. inflammation was measured (dry weight, protein content, myeloperoxidase ... | 1980 | 7391884 |
| intestinal distribution of trichinella spiralis in rats. | intestinal distribution of trichinella spiralis was studied in mature and suckling sprague-dawley rats. position of trichinella along the small intestine was defined by a median value for the population. a range, 90% of total population, gave a relatively precise determination of microhabitat width. the normal position of trichinella following an oral infection is in the duodenum, but position of trichinella in mature rats is more anterior than position of trichinella in suckling rats. intestina ... | 1980 | 7391890 |
| [frequency and significance of parasitic infections in refugees from vietnam (author's transl)]. | in the course of the medical care which is provided for the refugees from southeast asia 100 persons were tested for intestinal parasites (helminths and protozoa). in addition serum samples were obtained from 72 of these and tested in several tests (cft, fat, iha and sft) using antigens of entamoeba histolytica, leishmania donovani, toxoplasma gondii, schistosoma spp., fasciola hepatica, echinococcus spp., trichinella spiralis and litomosoides carinii. in 49 persons infections with one, two or ... | 1980 | 7395245 |
| requirement for two discrete stimuli for induction of the intestinal rapid expulsion response against trichinella spiralis in rats. | rats subjected to a 7-day abbreviated enteral infection with trichinella spiralis subsequently reject more than 90% of a challenge infection within 24 h. this process is known as rapid expulsion. in these experiments parabiotic rats were used to examine the factors that establish rapid expulsion in the intestine. induction with low to moderate doses of worms required exposure to two separate stimuli. these initiated different responses; one was readily transferred between parabiotic rats, wherea ... | 1980 | 7399701 |
| rapid expulsion of trichinella spiralis: coinduction by using antigenic extracts of larvae and intestinal stimulation with an unrelated parasite. | the injection of cell-free extracts of larval trichinella spiralis with freund complete adjuvant immunized rats against a challenge infection with t. spiralis. protection was expressed by a reduction of adult worms in the intestines 8 days after a challenge infection. the quantitatively more important rapid expulsion reaction could also be produced by immunization with antigen, provided immunized rats were also given an intestinal priming infection with adult trichinella spiralis or an unrelated ... | 1980 | 7399702 |
| concurrent infections of trichinella spiralis and toxoplasma gondii in mice. | concurrent infections with two parasites: a nematode, trichinella spiralis, and a protozoon. toxoplasma gondii, were investigated. antibody production (total immunoglobulin and igm) was similar in double and single infections. however, the number of toxoplasma cysts in the brains of mice infected with trichinella and challenged 1-6 weeks later with toxoplasma was higher than in mice infected with toxoplasma alone, while mice infected with toxoplasma and challenged 4-14 days later with trichinel ... | 1980 | 7415416 |
| preliminary studies on the course of a trichinella spiralis infection in athymic, nude rats. | | 1980 | 7420247 |
| distribution of trichinella spiralis in muscles of the mouse. | | 1980 | 7420250 |
| changing proteins on the surface of a parasitic nematode. | most of the organisms of the phylum nematoda are free living, but some are animal or plant parasites of major importance to man. during their life cycle all nematodes undergo a series of moults in which they shed an external cuticle, consisting of an outermost membrane-like layer of unknown composition and a series of fibrillar layers similar to collagens. because of this structure, the cuticle has been viewed as an acellular exoskeleton with rather inert molecular components. however, observati ... | 1980 | 7422005 |
| altered lysophospholipase b responsiveness in lactating mice infected with intestinal nematode parasites. | lactating and nulliparous outbred swiss (cf-1 strain) mice were infected at 12-16 weeks of age with nippostrongylus brasiliensis or trichinella spiralis. lysophospholipase b levels in the intestinal tissue and faecal pellets were greatly in infected lactating mice in contrast to infected nulliparous mice. correlated with these depressions in lysophospholipase levels were markedly reduced numbers of bone-marrow eosinophils in infected lactating mice. although the peak levels of lysophospholipase ... | 1980 | 7422360 |
| study of the kinetics of globule leucocytes in the intestinal epithelium of rats after single or double infection with trichinella spiralis. | in a previous study we suggested that intestinal globule leucocytes (gl) in the mouse represent a cell population independent of the intestinal mast cells (imc). in this paper we examined the interdependence of imc and gl in the rat and also the kinetics of gl in the intestinal epithelium. for this purpose rats received a single or double infection with the nematode trichinella spiralis. after the double infection worms were rapidly expelled. the numbers of imc and gl were related to the degree ... | 1980 | 7426383 |
| regional blood flow and the localization of lymphoblasts in the small intestine of the mouse. ii. the effects of a primary enteric infection with trichinella spiralis. | the localization of 125i-udr-labelled mesenteric lymph node cells in the small intestine and the fraction of the cardiac output delivered to this organ in mice has been examined. concurrent measurements of these two phenomena in normal animals showed that there was a significant correlation between the localization of lymphoblasts and the distribution of regional blood flow along the small intestine. in mice undergoing enteric infection with trichinella spiralis, however, the nature of the conne ... | 1980 | 7461718 |
| concurrent infections of hymenolepis diminuta and trichinella spiralis in the rat intestine. | the intestinal distribution of trichinella spiralis and hymenolepis diminuta in rats was studied in single and concurrent infections. results showed that the location of t. spiralis was not significantly changed in concurrent infections, but higher populations of t. spiralis in single and concurrent infections resulted in a posterior shift in position. trichinella spiralis affected h. diminuta distribution significantly by causing a posterior shift in the position of scoleces and biomass and a l ... | 1980 | 7463246 |
| the nonrandom, negative binomial distribution of experimental trichinellosis in rabbits. | the distribution of parasite populations in their hosts has been the subject of a number of mathematical analyses and it has been found that these distributions are generally nonrandom and overdispersed. data on the recovery of trichinella spiralis in the laboratory from 371 outbred rabbits were examined. the distribution of their parasite burden was overdispersed and best fit by a negative binomial frequency distribution. the intensity of the infections was almost totally independent of the dos ... | 1980 | 7463249 |
| [ultrastructural study of oogenesis in trichinella spiralis (author's transl)]. | the ultrastructural study of oogenesis in t. spiralis makes it possible to subdivide the oocytic growth into 2 phases: a first phase is characterized by an intense nucleolar activity and the transfer of nuclear material to the cytoplasm; a second phase during which the cytoplasmic mass increases and energy reserves (glycogen, lipids) appear. the oocyte does not contain any protein vitellus. | 1980 | 7469309 |
| effect of trichinella spiralis infection on the experimental amebic liver abscess in hamsters. | the development of experimental amebic liver abscess in hamsters was inhibited by a previous infection with the nematode parasite trichinella spiralis, such that liver abscesses were present in 26% of hamsters with t. spiralis infection and in 80% of t. spiralis-free controls. the protective effect of t. spiralis infection is believed to be exerted through a non-specific activation of cellular immunity, with macrophages as probable effector cells. | 1980 | 7469639 |
| detection of trichinella spiralis muscle larvae in naturally infected horses. | human trichinellosis outbreaks related to horsemeat consumption have been reported in france and italy in recent years. in order to determine if trichinella is present in horses slaughtered at an abattoir in the state of mexico, diaphragm muscle tissue samples (22-37 g) from 80 horses were examined by artificial digestion. four of these samples had larvae that were characterized as trichinella sp. by morphological criteria and as trichinella spiralis by the polymerase chain reaction. | 1995 | 7472874 |
| [the distribution of trichinella nativa, t. nelsoni and t. pseudospiralis in eurasia]. | a hundred and forty-six isolates of trichinella from various regions of eurasia have been identified by the crossbreeding method (1 male + 1 female). three independent species of trichinella: t. nativa, t. nelsoni, and t. pseudospiralis were found to parasite on the animals of this continent. the former two are dominant species of different territories. t. nativa occupies the whole continental zone to the north and the north-east of the isotherm minus 5-6 degrees c in january and t. nelsoni popu ... | 1995 | 7476675 |
| [the synthesis and study of the acute toxicity and anthelmintic activity of new 2-methylthiobenzimidazoles]. | several derivatives of 2-methylthiobenzimidazoles were synthesized and assayed for anthelmintic activity. following these studies, 2-methylthio-5-(1-chloronaphthyloxy-2)-6-chlorobenzimidazole (g-1557) has been selected as a tichinellacide for detailed investigations. | 1995 | 7476681 |
| purification and preliminary characterization of a protease from the excretion-secretion products of trichinella spiralis muscle-stage larvae. | a protease from excretion-secretion products of trichinella spiralis muscle-stage larvae was purified by continuous elution electrophoresis. the state of purification was analyzed electrophoretically using one- and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. the purified enzyme was shown to be a single polypeptide with an estimated molecular mass of 35 kda and isoelectric point of 6.2. following purification, the enzyme activity was measured by hydrolysis of gelati ... | 1995 | 7483238 |
| [characterization of surface antigens of the nematode parasite trichinella spiralis: study of its role in protection mechanisms and their usefulness in the diagnosis of trichinosis]. | among the most important aspects in the study of trichinosis are the development of specific and sensitive diagnostic methods for the detection of the infection by the parasite as well as the characterization of antigens from trichinella spiralis that induce protection in the host. in the context, the characterization of surface stichosome and excretory secretory antigens of t. spiralis muscle larvae has been an important issue due to the high immunogenicity of such components in most hosts so f ... | 1995 | 7493736 |
| [edema of the lower limbs revealing trichinosis identified by duodenal biopsy]. | | 1995 | 7501630 |
| patterns of cytokine mrna in trichinella spiralis infected rats. | | 1995 | 7502935 |
| interleukin 1 beta-induced increase in substance p in rat myenteric plexus. | substance p (sp) is increased in the inflamed intestine of trichinella spiralis-infected rats, but the underlying mechanism is unknown. interleukin 1 beta (il-1 beta) messenger rna and protein is expressed in the longitudinal muscle-myenteric plexus (lm-mp) of this model. thus, the purpose of the study was to examine the ability of human recombinant il-1 beta (hril-1 beta) to increase sp in lm-mp preparations from the intestine of noninfected rats. | 1993 | 7504644 |
| structural and molecular changes in intestinal smooth muscle induced by trichinella spiralis infection. | infection with trichinella spiralis in the rat causes altered intestinal motility and jejunal smooth muscle contractility by day 6 postinoculation. the purpose of this study was to determine structural and molecular changes in the smooth muscle that could account for the functional changes that have been reported. by day 6 postinoculation, there was an increase in thickness of both muscle layers of the jejunum. this increase in mass was accompanied by an increase in total protein content of the ... | 1994 | 7515575 |
| histochemical and morphological studies on trichinella spiralis larvae treated with high hydrostatic pressure. | histochemical and morphological observations were made on trichinella spiralis larvae treated with hydrostatic pressures of 100, 150, 200 and 300 mpa using hematoxylin-eosin (he), periodic acid-schiff (pas) and azan staining. few histochemical changes were observed in he and pas stained larvae after pressurization at 200 mpa and under. however, red staining by azan changed to blue in the anterior stichosome of larvae and skeletal muscle of mice, when the hydrostatic pressure was raised from 150 ... | 1994 | 7520888 |
| intestinal anaphylaxis: radiation-induced suppression. | the gastrointestinal tract is highly sensitive to ionizing radiation. some of the most radiosensitive cells in this system are mast cells and epithelium. this article describes experiments that test the hypothesis that irradiation suppresses mucosal immune responses in which mast cells and epithelium are involved. the hypothesis was tested by examining the impact of ionizing radiation on anaphylactically mediated cl- secretion in jejunum of rats sensitized by trichinella spiralis infection and c ... | 1994 | 7524351 |
| neuroimmune communication in the submucous plexus of guinea pig colon after infection with trichinella spiralis. | enteric neuroimmune communication in gastrointestinal hypersensitivity responses includes antigen detection by mast cells and release of chemical messages to the enteric nervous system. the aim of this study was to analyze the electrical and synaptic behavior of neurons in the colonic submucous plexus during exposure to trichinella spiralis antigen in animals infected earlier with the parasite. | 1994 | 7525397 |
| molecular analysis of antigens targeted by protective antibodies in rapid expulsion of trichinella spiralis. | rapid expulsion is a protective immune mechanism which eliminates as much as 99% of a challenge infection of trichinella spiralis muscle larvae from the gastrointestinal tract of suckling rats. protective monoclonal antibodies (mabs) generated against larval excretory-secretory antigens (esa) specifically recognize a 45-kda glycoprotein, gp45, in addition to a distinct profile of other cross-reactive antigens that are also recognized by non-protective mabs. recent data indicate that protective m ... | 1994 | 7526209 |
| altered neuropeptide content and cholinergic enzymatic activity in the inflamed guinea pig jejunum during parasitism. | we investigated the effects of an enteric infection with the parasitic nematode, trichinella spiralis, on peptidergic and cholinergic neural pathways of the guinea pig jejunum. the content of the enteric neuropeptides, substance p (sp) and vasoactive intestinal peptide (vip), and the activities of the key cholinergic enzymes, acetylcholinesterase (ache) and choline acetyltransferase (chat), were measured and compared in extracts of jejunal muscularis externa (me) obtained from uninfected jejunum ... | 1995 | 7541515 |
| stem cell factor contributes to intestinal mucosal mast cell hyperplasia in rats infected with nippostrongylus brasiliensis or trichinella spiralis, but anti-stem cell factor treatment decreases parasite egg production during n brasiliensis infection. | we assessed the effects of the c-kit ligand, stem cell factor (scf), in the jejunal mucosal mast cell hyperplasia that occurs during infection with the intestinal nematodes, nippostrongylus brasiliensis or trichinella spiralis in rats. compared with vehicle-treated rats, rats treated with scf (25 micrograms/kg/d, intravenous [i.v.] for 14 days) during n brasiliensis infection exhibited significantly higher levels of the rat mucosal mast cell (mmc)-associated protease, rat mast cell protease ii ( ... | 1995 | 7544650 |
| substance-p-mediated intestinal inflammation: inhibitory effects of cp 96,345 and sms 201-995. | the proinflammatory peptide substance p (sp) has been shown to be intimately involved in the local inflammatory processes of trichinella-spiralis-induced murine intestinal inflammation. significant increases in sp, increased myeloperoxidase levels coupled with local morphological deterioration of the jejunum and impaired lymphocyte responses to exogenous sp in vitro have been associated with the model. we have recently determined that the elimination of increased levels of sp via anti-sp antibod ... | 1994 | 7545531 |
| progesterone modulation of anaphylactically induced ion transport in uterus and intestine. | the objective of this study was to investigate the role of progesterone in modulating electrophysiological responses to antigenic challenge that are reflective of immune functions in uterine and jejunal mucosae. in the rat-trichinella spiralis, host-parasite system, an enteric infection with a microscopic nematode sensitizes both the jejunum and uterus. electrophysiological correlates of local anaphylaxis or type i hypersensitivity were monitored in vitro throughout the estrus cycle and during p ... | 1995 | 7549500 |
| suppression of transplant immunity in experimental trichinellosis. | skin allograft rejection in balb/c and c57bl/6j mice following experimental infection with 300 larvae of trichinella spiralis or trichinella pseudospiralis was studied. skin grafts from normal c57bl/6j mice were transplanted to infected balb/c mice and vice versa at days 3, 10, 20 and 30 post-infection. the clinical criteria for graft rejection, scarring and graft falling, were followed. the results indicated that t. spiralis and t. pseudospiralis infections induced a significant delay in graft ... | 1995 | 7554818 |
| antibody response to a protease secreted by trichinella spiralis muscle larvae. | in the present study we analyzed the humoral response of trichinella spiralis-infected mice to a 35-kda protease (purified from the excretory-secretory products of t. spiralis muscle larvae) by a western-blot procedure and an enzyme-linked immunosorbent assay (elisa) technique using a panel of postinfection mouse anti-trichinella sera. the results demonstrated that this response was time-dependent and that infected mice could be distinguished from controls. in addition, inhibition assays demonst ... | 1995 | 7567916 |
| prevalence of igg antibodies to trichinella spiralis in dogs in macedonia, northern greece. | an enzyme-linked immunosorbent assay (elisa) was used to detect t. spiralis infection in dogs, using larval t. spiralis excretory-secretory (es) antigen. forty-three (4.3%) dog sera out of 1000 revealed the presence of igg t. spiralis. the positive sera were distributed in three groups; 21 (2.1%) weakly positive, 14 (1.4%) moderately positive, and eight (0.8%) strongly positive. | 1995 | 7571342 |
| short report: an outbreak of trichinosis in navarra, spain. | trichinosis is an infection contracted by ingestion of meat containing viable larvae of the nematode trichinella spiralis. this report concerns an outbreak of infections with this parasite in navarra, spain that was associated with home-prepared pork products. after the detection of a person with trichinosis, a study of all subjects that had ingested meat from the presumably infected pork was carried out. forty-four members of eight families were enrolled in the study. ten had symptoms suggestiv ... | 1995 | 7573704 |
| a study on patients with eosinophilia of suspected parasitic origin. | a study of 53 patients with eosinophilia of suspected parasitic origin was conducted. investigations done for each case included, stool and urine examinations, absolute eosinophilic count and countercurrent immunoelectrophoresis (ciep) using crude antigens of fasciola hepatica, schistosoma mansoni worms, trichinella spiralis and toxocara canis larvae, and hydatid fluid. stool examination revealed six cases with fasciola eggs, two with ascaris lumbricoides and one with s. mansoni eggs. the eosino ... | 1995 | 7602166 |
| a method for isolation and partial purification of trichinella spiralis nurse cells. | invasion of vertebrate muscle cells by larvae of trichinella spiralis is accompanied by redifferentiation of the host myofiber into a novel structure called the nurse cell. the nurse cell protects and nurtures the enclosed parasite during its long stay in host muscle. it is anatomically independent of the surrounding uninfected muscle cells and can be isolated from host tissue by mechanical or enzymatic means. current methods employed for this purpose have yielded only small numbers of nurse cel ... | 1995 | 7623214 |
| [trichinosis outbreak in purranque county, x region, chile. october-november, 1992]. | an outbreak of trichinosis occurred in purranque county, x region, chile, between october and november of 1992, which involved 36 persons. the incubation period, determined by the clinical picture and laboratory assays, fluctuated between 10 and 12 days. myalgias (88.9%) and palpebral oedema (86.1%), were the most important symptoms, followed by fever (44.4%) and headache (33.3%). eosinophils count ranged from two to 42% the first week, and this value raised to 55% the second week of the outbrea ... | 1994 | 7632339 |
| [epidemiology of trichinosis in chile. prevalence study by immunodiagnostic reactions]. | in the period 1983-1994 a series of seroepidemiological surveys, by using immunodiagnostic tests for trichinosis, was carried out in 138 localities and health institutions from the 13 regions of the country. thus, a total of 12,882 randomly selected persons, with different sex and age distribution, were submitted to precipitin test and indirect hemagglutination test for trichinosis. one hundred and ninety one (1.5%) persons resulted positive for trichinosis. higher rates of infection were observ ... | 1994 | 7654290 |
| experimental colitis alters myenteric nerve function at inflamed and noninflamed sites in the rat. | studies in inflammatory bowel disease have shown extensive structural abnormalities in the enteric nervous system of inflamed and noninflamed gut; however, functional correlates are lacking. the aim of this study was to determine the effect of colitis on myenteric nerve function at inflamed and noninflamed sites in rat intestine. | 1995 | 7657099 |
| the effect of flubendazole on the course of trichinella spiralis infection in mice: parasitological study. | this work planned to study the effect of flubendazole against different stages of trichinella spiralis infection in mice. three groups of experimental animals were examined: control group, group i treated three days post infection and group ii treated four week post infection. in group i, flubendazole was shown to be a very potent agent in killing trichinella spiralis adults and larvae, when given orally in a dose of 20 mg/kg for five consecutive days. this is evidenced by the significant reduct ... | 1995 | 7665941 |
| inhibition of neutrophil oxidative metabolism by trichinellosis patient sera. parasite origin or host induction? | the presence of sera factors able to inhibit both neutrophil chemotaxis and phagocytosis was observed in all patients studied at two months from infection caused by trichinella britovi and in most of them after one year. human neutrophils with eosinophils are able to kill t. spiralis newborn larvae in an adcc system and their major cytotoxic mechanism is oxidative metabolism products. we evaluated the effect of trichinellosis sera on neutrophil oxidative burst to determine if neutrophils are aff ... | 1995 | 7675512 |
| proteinases in the excretory/secretory products (es) of adult trichinella spiralis. | adult trichinella spiralis were maintained in vitro using defined media and the material excreted/secreted (es) during this time examined for proteolytic enzyme (proteinase) activity using an azocasein assay and gelatin-substrate gels. several discrete proteinases in the size range 14-100 kda were observed with optimal activity at ph 7.5. the use of a class-differentiating panel of proteinase inhibitors indicated that serine proteinases were predominant although some inhibition was evident in th ... | 1995 | 7675535 |
| characteristics of t lymphocyte cell lines established from nih minipigs challenge inoculated with trichinella spiralis. | mhc restricted, antigen specific, cytokine dependent t-cell lines were established from peripheral blood mononuclear cells (pbmc) of nih minipigs that were challenge inoculated with trichinella spiralis. swine lymphocyte antigen (sla) inbred nih minipigs of the sla a/a (aa) haplotype can be divided into responder (r) and non-responder (nr) phenotypes on the basis of their ability to destroy encysted muscle larval (ml) forms of the parasite t. spiralis. when orally inoculated with 300 infectious ... | 1993 | 7679239 |
| the in vivo role of stem cell factor (c-kit ligand) on mastocytosis and host protective immunity to the intestinal nematode trichinella spiralis in mice. | the role of stem cell factor (scf) in the generation of intestinal mast cell hyperplasia and host protective immunity following helminth infection was investigated using the trichinella spiralis/mouse model. in vivo administration of a monoclonal antibody specific for the receptor for scf (c-kit) was found to completely prevent the generation of intestinal mastocytosis normally observed following t. spiralis infection. this was reflected by markedly reduced intestinal mast cell protease (imcp) l ... | 1993 | 7679484 |
| identification of potential antigenic targets for rapid expulsion of trichinella spiralis. | rat monoclonal antibodies, which have been shown to mediate rapid expulsion of trichinella spiralis in infant rats, were used in immunochemical analyses to identify the antigens targeted in protection. protective antibodies bound to gp45, and to a series of related antigens, in the excretory-secretory products of muscle stage larvae. non-protective antibodies did not bind gp45, but did bind the related species. the latter were shown to be present as aggregates (dimers and multimers) in excretory ... | 1993 | 7681546 |
| altered expression of sodium pump isoforms in the inflamed intestine of trichinella spiralis-infected rats. | we used polymerase chain reaction (pcr) and northern analysis to examine the effect of trichinella spiralis infection on mrna expression of sodium pump isoforms in rat intestine. 28s rna was coamplified as an internal standard, and the abundance of the isoformic mrna was expressed relative to 28s rna. as assessed by pcr and northern analysis, the alpha 1- and beta 1-subunits were expressed in the jejunum and ileum of noninfected control rats. however, in the jejunum the levels were suppressed af ... | 1993 | 7687413 |
| regional content of enteric substance p and vasoactive intestinal peptide during intestinal inflammation in the parasitized ferret. | our aim was to characterize and quantitate changes in two key neuropeptides, substance p (sp) and vasoactive intestinal peptide (vip), that are involved in governing neurally-mediated gastrointestinal (gi) reflex activity during enteric inflammation in the ferret. neuropeptide content was determined by radioimmunoassay of extracts of jejunal, ileal and colonic muscularis externa from uninfected ferrets and ferrets infected with enteric stages of the parasitic nematode, trichinella spiralis. incr ... | 1993 | 7692345 |
| inhibition of murine intestinal inflammation by anti-substance p antibody. | several neuropeptides have recently been shown to affect various aspects of the inflammatory process. among these, the neuropeptide substance p possesses a host of immune modifying actions, which include the enhancement of lymphocyte activity, macrophage function, and neutrophil chemotaxis. the role of substance p during inflamed states has, as yet, not been fully described. here, in t. spiralis-infected mice, we parallel increased levels of substance p both locally, (the gut) and peripherally ( ... | 1993 | 7692913 |
| studies on the rate of selective uptake of amino acids by trichinella larvae in vivo. | groups of c57bl/6j mice, orally infected with 300 larvae each of trichinella spiralis or t. pseudospiralis were injected with [3h]-alanine, tyrosine, tryptophan or glycine. the incorporation of isotope labelled amino acids into larval proteins was measured at 2, 6, and 12 months post-infection. it was shown that there is a significant increase in the in vivo uptake of isotope labelled amino acids with time by the larvae of t. spiralis and t. pseudospiralis. the level of uptake was highest for ty ... | 1994 | 7706672 |
| the response of hamsters to primary and secondary infection with trichinella spiralis and to vaccination with parasite antigens. | the duration of primary infections with t. spiralis was dose-dependent with greater proportional loss of worms from heavily infected hamsters and longer persistence of worms in syngeneic dsn hamsters carrying initially low intensity infections. intestinal worms were lost more rapidly from challenged immunized animals with over 80% loss of established worms by day 6 post infection, but survival of residual worms for a further 2 weeks. hamsters carrying initially more than 140 intestinal worms beg ... | 1994 | 7706673 |
| ultrastructural study of trichinella spiralis with emphasis on adult male reproductive organs. | the ultrastructure of the reproductive system of adult male trichinella spiralis has been examined, particularly to assist in recent advances such as the localization of target antigens of protective immunity and the mode of immune attack. the male reproductive system consists of a single tube with a hairpin-like bend, composed of a basal lamina, epithelial cells, rachis, circumferential and constrictor muscles, and germinal cells. the organs were surrounded by basal lamina and haemolymph. germi ... | 1994 | 7706686 |
| characterization of the recognition specificity of autoreactive, polyspecific monoclonal antibodies obtained from spleen cells of parasite-infected balb/c mice. | we examined the specificity of four monoclonal antibodies (moabs) obtained from schistosoma japonicum (1d, gao3), trypanosoma cruzi (tslo), and trichinella spiralis (tsy2) infected balb/c mice. all four moabs reacted not only with autologous parasite antigens but also with various heterologous parasite antigens and normal tissues. the antigen recognition pattern seen on western blots was almost identical in 1d and gao3, and in tslo and tsy2. furthermore, certain bands were identical among all fo ... | 1994 | 7708427 |
| effect of three immunostimulants on the resistance against trichinella spiralis infection in mice. (preliminary report). | twenty eight nih mice were divided into four groups of seven animals each. mice in groups were injected intraperitoneally with freund's complete adjuvant (fca), concanavalin a (con a), equimune (ei) and phosphate buffered saline (pbs), respectively, 7 days before infection with 300 trichinella spiralis infective larvae per animal. forty two days after infection all mice were sacrificed and processed by artificial digestion to determine the number of larvae per gram (lpg) of muscle tissue. the me ... | 1995 | 7711455 |
| [the specific and biological action of chemical preparations and their combination with pathogenetic agents in trichinosis]. | the hypersensitizing effect of benzimidazole carbamates (bc), the therapy for trichinellosis, especially in heavily infected patients, determines the necessity of simultaneous administration of glucocorticoids (gc) for the prevention of severe systemic and organ-specific complications. this combination delayed the convalescent period, in severe cases it provokes coagulopathic disorders, infrequently fatal haemorrhages. the combination of bc with nonsteroidal anti-inflammatory drugs (nsaids) sign ... | 1994 | 7715560 |
| investigation of cross-reactions against trichinella spiralis antigens by enzyme-linked immunosorbent assay and enzyme-linked immunoelectrotransfer blot assay in patients with various diseases. | data regarding cross-reactions against trichinella spiralis in humans are scarce and controversial. for this reason, we tested serum samples from patients with typhoid fever, brucellosis, toxoplasmosis, amoebiasis, cysticercosis, trichocephaliasis, ascariasis, and onchocerciasis against an antigenic extract of t. spiralis infective larvae in an enzyme-linked immunosorbent assay (elisa) and an enzyme-linked immunoelectrotransfer blot (eitb) assay. all except one serum sample from the group of pat ... | 1995 | 7719905 |
| detection of a circulating parasitic antigen in acute infections with trichinella spiralis: diagnostic significance of findings. | circulating parasitic antigen was demonstrated in the sera of rats and patients infected with trichinella spiralis. using a dot-immunobinding method, the antigen was detected in the sera of experimental animals from the fourth day of infection and in more than one third of the patients by the end of the third week from infection. the method proved to be simple, sensitive and specific. it should assist an early diagnosis of trichinellosis demonstrating the antigen in some cases with negative or d ... | 1994 | 7727900 |
| trichinella spiralis: cloning and characterisation of two repetitive dna sequences. | digestion of genomic dna of trichinella spiralis with ecori reveals the presence of several families of repetitive dna. two small repeats of 516 and 604 bp, respectively, have been cloned and sequenced. the two repeats are related to each other and to a 1.1-kb repetitive dna. similar sequences are not detected in other species by hybridisation. there are about 270 copies of each small repeat per haploid genome and these are organised in large arrays of > or = 50 kb. the basic repetitive unit app ... | 1995 | 7729484 |
| host resistance to trichinella spiralis infection in rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd). | we have previously shown decreased resistance to trichinella spiralis (ts) infection and reduced parasite antigen-specific responses in b6c3f1 mice exposed to tcdd before infection. the current study was done to characterize the effects of preinfection administration of 1, 10, or 30 micrograms tcdd/kg on host resistance of female f344 rats to ts infection and to examine parasite antigen-specific responses in the spleen and mesenteric lymph nodes of infected animals. tcdd exposure did not affect ... | 1995 | 7737439 |
| [chronic endometriosis caused by trichinella spiralis. report of a case]. | a case of endometrial trichinosis is reported; the patient lived rear cd. delicias, chih., an endemic area for trichinosis. a 38 years old obese, diabetic woman, with high blood pressure, used to eat frequently pork meat and its products; she presented with abnormal genital bleeding, abdominal pain, and pelvic congestion. histerectomy was performed. pathological study of the uterus showed trichinella spiralis larvae with microcalcifications, and lymphocytic infiltration in basal endometrium. as ... | 1995 | 7744289 |
| [an outbreak of trichinosis in huerta del marquesado (the cañete-cuenca basic health area). december 1992 to january 1993]. | a trichinellosis outbreak was investigated (december 1992-january 1993). it occurred in the village of huerta del marquesado among people who consumed sausages made with pork, meat. the aim of this study is to show the consequences of consuming meat without a sanitary control on human health. | 1994 | 7747072 |