| establishment of an efficient transformation system for pleurotus ostreatus. | pleurotus ostreatus is widely cultivated worldwide, but the lack of an efficient transformation system regarding its use restricts its genetic research. the present study developed an improved and efficient agrobacterium tumefaciens-mediated transformation method in p. ostreatus. four parameters were optimized to obtain the most efficient transformation method. the strain lba4404 was the most suitable for the transformation of p. ostreatus. a bacteria-to-protoplast ratio of 100:1, an acetosyring ... | 2017 | 29164387 |
| differential expression patterns of pleurotus ostreatus catalase genes during developmental stages and under heat stress. | catalases are ubiquitous hydrogen peroxide-detoxifying enzymes. they participate in fungal growth and development, such as mycelial growth and cellular differentiation, and in protecting fungi from oxidative damage under stressful conditions. to investigate the potential functions of catalases in pleurotus ostreatus, we obtained two catalase genes from a draft genome sequence of p. ostreatus, and cloned and characterized them (po-cat1 and po-cat2). po-cat1 (group ii) and po-cat2 (group iii) enco ... | 2017 | 29160795 |
| development of novel polymorphic est-ssr markers in bailinggu (pleurotus tuoliensis) for crossbreeding. | identification of monokaryons and their mating types and discrimination of hybrid offspring are key steps for the crossbreeding of pleurotus tuoliensis (bailinggu). however, conventional crossbreeding methods are troublesome and time consuming. using rna-seq technology, we developed new expressed sequence tag-simple sequence repeat (est-ssr) markers for bailinggu to easily and rapidly identify monokaryons and their mating types, genetic diversity and hybrid offspring. we identified 1110 potentia ... | 2017 | 29149037 |
| efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports. | different technologies may be used for decolorization of wastewater containing dyes. among them, biological processes are the most promising because they seem to be environmentally safe. the aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (pleurotus ostreatus - bwph, gleophyllum odoratum - dca and polyporus picipes - rwp17). different solid supports were teste ... | 2017 | 29129408 |
| anti-invasive and antiproliferative effects of pleurotus ostreatus extract on acute leukemia cell lines. | currently, mushrooms have been used in traditional and folk medicines for their therapeutic activities, such as antibiotic, antitumor, anti-inflammatory, anticancer, antileukemic and immunomodulatory actions. this investigation evaluates the anti-invasive, antiproliferative and cytotoxic effects of pleurotus ostreatus (pleurotaceae) on leukemia cell lines. | 2018 | 29127762 |
| differential gene expression profiling analysis in pleurotus ostreatus during interspecific antagonistic interactions with dichomitus squalens and trametes versicolor. | this study provided analysis of differentially expressed genes (degs) in pleurotus ostreatus under the interaction with dichomitus squalens and trametes versicolor, which is valuable for exploration on the fungal defence system against stressful condition caused by interspecific antagonistic interaction. our result showed significant upregulation of abundant defence-related genes encoding laccase, manganese peroxidase, aldo-keto reductase, and glutathione s-transferase, which all play important ... | 2017 | 29122174 |
| a role for small secreted proteins (ssps) in a saprophytic fungal lifestyle: ligninolytic enzyme regulation in pleurotus ostreatus. | small secreted proteins (ssps), along with lignocellulose degrading enzymes, are integral components of the secretome of pleurotus ostreatus, a white rot fungus. in this study, we identified 3 genes (ssp1, 2 and 3) encoding proteins that are annotated as ssps and that exhibited of ~4,500- fold expression, 24 hr following exposure to the toxic compound 5-hydroxymethylfurfural (hmf). homologues to genes encoding these ssps are present in the genomes of other basidiomycete fungi, however the role o ... | 2017 | 29109463 |
| heterologous expression and characterization of three laccases obtained from pleurotus ostreatus haucc 162 for removal of environmental pollutants. | chlorophenols (cps), nitrophenols (nps), and sulfonamide antibiotics (sas) are three types of environmental pollutants that are of great concern because of their prevalence and toxicity. in this study, three laccase isoenzymes obtained from pleurotus ostreatus haucc 162 were heterologously expressed and characterized with respect to their ability to degrade cps, nps, and, sas. the three recombinant laccases can efficiently degrade the three types of considered pollutants using a laccase-mediator ... | 2018 | 29100130 |
| antimicrobial, antioxidant and anticancer activities of zinc nanoparticles prepared by natural polysaccharides and gamma radiation. | aqueous dispersed zinc nanoparticles (znnps) were synthesized using natural polysaccherides (chitosan (cs), citrus pectin (cp) and alginate (alg)) using aqueous fermented fenugreek powder (ffp) by pleurotus ostreatus as reducing and stabilizing agent or using gamma irradiation. the synthesized znnps are characterized by ultra violect spectroscopy (uv), transmission electronmicroscopy (tem), dynamic light scattering (dls), x-ray diffraction (xrd), and fourier transform infrared spectroscopy (ft-i ... | 2018 | 29097216 |
| impact of optimised cooking on the antioxidant activity in edible mushrooms. | this study aimed to investigate the effect of four cooking methods with different durations on the in vitro antioxidant activities of five edible mushrooms, namely agaricus bisporus, flammulina velutipes, lentinula edodes, pleurotus ostreatus and pleurotus eryngii. among the raw samples, a. bisporus showed the highest total antioxidant activity (reducing power and radical scavenging), total flavonoid, ascorbic acid and water soluble phenolic contents. short-duration steam cooking (3 min) increas ... | 2017 | 29085153 |
| batch and fixed-bed biosorption of cd(ii) from aqueous solution using immobilized pleurotus ostreatus spent substrate. | to prevent the blockage in a continuous fix-bed system, pleurotus ostreatus spent substrate (poss), a composite agricultural waste, was immobilized into granular adsorbents (iposs) with polymeric matrix, and used to remove cd(ii) from synthetic wastewater in batch experiment as well as in continuous fixed-bed column system. in batch experiment, higher ph, temperature and cd(ii) initial concentration were conducive to a higher biosorption capacity, and the maximum biosorption capacity reached up ... | 2018 | 29080541 |
| antidiabetic activity of ergosterol from pleurotus ostreatus in kk-ay mice with spontaneous type 2 diabetes mellitus. | the number of people with diabetes is increasing rapidly in the world. in the present study, the hypoglycemic activity and potential mechanism of ergosterol (erg), a phytosterol derived from the edible mushroom pleurotus ostreatus are investigated in vitro and in vivo. | 2017 | 29080247 |
| high temperature enhances the ability of trichoderma asperellum to infect pleurotus ostreatus mycelia. | trichoderma asperellum is one of the species which can be isolated from contaminated pleurotus ostreatus cultivation substrate with green mold disease. this study focused on the relationship between high temperature and infectivity of t. asperellum to p. ostreatus. antagonism experiments between t. asperellum and p. ostreatus mycelia revealed that high temperature-treated p. ostreatus mycelia were more easily infected by t. asperellum and covered by conidia. microscopic observation also showed t ... | 2017 | 29073211 |
| effects of pex1 disruption on wood lignin biodegradation, fruiting development and the utilization of carbon sources in the white-rot agaricomycete pleurotus ostreatus and non-wood decaying coprinopsis cinerea. | peroxisomes are well-known organelles that are present in most eukaryotic organisms. mutant phenotypes caused by the malfunction of peroxisomes have been shown in many fungi. however, these have never been investigated in agaricomycetes, which include white-rot fungi that degrade wood lignin in nature almost exclusively and play an important role in the global carbon cycle. based on the results of a forward genetics study to identify mutations causing defects in the ligninolytic activity of the ... | 2017 | 29030267 |
| the identification of transcriptional regulation related gene of laccase poxc through yeast one-hybrid screening from pleurotus ostreatus. | pleurotus ostreatus laccase gene poxc is transcriptionally induced by copper, and several putative mres have been found and confirmed in its promoter region. however, the related transcription factors mediating copper response via mres have not been reported. to isolate mre binding proteins, we carried out yeast one-hybrid screens. reporter genes containing two trimers of the cmre2 and cmre3 element were prepared and introduced into a yeast strain. the yeast was transformed with library cdna tha ... | 2017 | 29029697 |
| correction to: malachite green and crystal violet decolorization by ganoderma lucidum and pleurotus ostreatus supernatant and by rgllcc1 and rpoxa 1b concentrates: molecular docking analysis. | the original version of this article unfortunately contained a mistake. the replacement image of fig. 4 provided by the first corresponding author, aura m. pedroza-rodríguez, is incorrect and that the originally submitted fig. 4 should have been retained. the original article has been corrected. | 2017 | 28990133 |
| lignocellulosic waste valorisation strategy through enzyme and biogas production. | lignocellulosic wastes are generally pre-treated to facilitate the hydrolysis stage during the anaerobic digestion process. a process consisting of solid state fermentation carried out by white rot fungi and anaerobic digestion was evaluated on corn stover to produce ligninolytic enzymes and biogas. the enzyme production was quantified every 3d for a month at 30°c, and three fungal strains and two particle sizes of waste were compared. of the main outcomes, pleurotus eryngii produced the highest ... | 2018 | 28961446 |
| aqueous extracts of wild mushrooms show antimicrobial and antiadhesion activities against bacteria and fungi. | mushrooms represent promising sources of novel bioactive compounds and can be applied as innovative strategies to control microbial contamination and infection via the food chain. we characterized aqueous extracts from 21 wild basidiomycete mushrooms and the cultivated oyster mushroom, pleurotus ostreatus, as putative sources of antimicrobial and antiadhesive compounds. broth microdilutions and adhesion to a polystyrene surface were evaluated on gram-positive and gram-negative bacteria and on fu ... | 2017 | 28960508 |
| nutritional quality of oyster mushroom (pleurotus ostreatus) as affected by osmotic pretreatments and drying methods. | this study was conducted to evaluate the effects of different levels of osmotic pretreatments prior to drying and different drying methods on nutritional quality of dried mushroom slices. the experiment consisted of sun, solar, and oven drying after dipping the slices in salt solutions of 5 and 10% concentrations for 50 minutes, the control being untreated mushroom sample. significant differences in proximate composition were observed between the fresh and dried mushroom samples. the average mea ... | 2017 | 28948016 |
| new lipases by mining of pleurotus ostreatus genome. | the analysis of pleurotus ostreatus genome reveals the presence of automatically annotated 53 lipase and 34 carboxylesterase putative coding-genes. since no biochemical or physiological data are available so far, a functional approach was applied to identify lipases from p. ostreatus. in the tested growth conditions, four lipases were found expressed, with different patterns depending on the used c source. two of the four identified proteins (pleolip241 and pleolip369), expressed in both analyse ... | 2017 | 28945798 |