| production of succinic acid from sugarcane molasses supplemented with a mixture of corn steep liquor powder and peanut meal as nitrogen sources by actinobacillus succinogenes. | the potential of using corn steep liquor powder (cslp), peanut meal (pm), soybean meal (sm), cotton meal (cm) and urea as the substitute of yeast extract (ye) as the nitrogen source was investigated for producing succinic acid (sa). actinobacillus succinogenes gxas137 was used as the fermenting bacterium and sugarcane molasses was used as the main substrate. none of these materials were able to produce sa as high as ye did. the cslp could still be considered as a feasible and inexpensive alterna ... | 2015 | 25647487 |
| biotechnological potential of yeast isolates from cachaça: the brazilian spirit. | this study identified phenotypic traits appropriate for biotechnological applications of 118 yeasts isolated from cachaça distilleries. different properties were verified: capacity to use alternative carbon sources; ability to tolerate high concentrations of sucrose, ethanol, methanol, aluminum and zinc as well as different ph values and foam production. pichia guilliermondii and pichia anomala strains were identified as the most promising ones for application in the second-generation biofuel in ... | 2015 | 25540045 |
| hemicellulosic ethanol production by immobilized cells of scheffersomyces stipitis: effect of cell concentration and stirring. | bioconversion of hemicellulosic hydrolysate into ethanol plays a pivotal role in the overall success of biorefineries. for the efficient fermentative conversion of hemicellulosic hydrolysates into ethanol, the use of immobilized cells system could provide the enhanced ethanol productivities with significant time savings. here, we investigated the effect of 2 important factors (e.g., cell concentration and stirring) on ethanol production from sugarcane bagasse hydrolysate using the yeast scheffer ... | 2015 | 25488725 |
| mineral composition of the sugarcane juice and its influence on the ethanol fermentation. | in the present work, we evaluated the mineral composition of three sugarcane varieties from different areas in northeast brazil and their influence on the fermentation performance of saccharomyces cerevisiae. the mineral composition was homogeneous in the different areas investigated. however, large variation coefficients were observed for concentrations of copper, magnesium, zinc and phosphorus. regarding the fermentation performances, the sugarcane juices with the highest magnesium concentrati ... | 2015 | 25248994 |
| ethanol production from agricultural wastes using saccharomyces cerevisiae. | the main objective of this study was production of ethanol from three lignocellulosic biomasses like sugarcane bagasse, rice straw and wheat straw by sacchromyces cervisae. all the three substrates were ground to powder form (2 mm) and pretreated with 3%h2o2 + 2% naoh followed by steaming at 130 °c for 60 min. these substrates were hydrolyzed by commercial cellulase enzyme. the whole fermentation process was carried out in 500 ml erlenmeyer flask under anaerobic conditions in submerged fermentat ... | 2014 | 25242928 |
| mathematical modeling of a continuous alcoholic fermentation process in a two-stage tower reactor cascade with flocculating yeast recycle. | experiments of continuous alcoholic fermentation of sugarcane juice with flocculating yeast recycle were conducted in a system of two 0.22-l tower bioreactors in series, operated at a range of dilution rates (d 1 = d 2 = 0.27-0.95 h(-1)), constant recycle ratio (α = f r /f = 4.0) and a sugar concentration in the feed stream (s 0) around 150 g/l. the data obtained in these experimental conditions were used to adjust the parameters of a mathematical model previously developed for the single-stage ... | 2015 | 25234511 |
| variable flocculation profiles of yeast strains isolated from cachaça distilleries. | in cachaça production, the use of yeast cells as starters with predictable flocculation behavior facilitates the cell recovery at the end of each fermentation cycle. therefore, the aim of this work was to explain the behavior of cachaça yeast strains in fermentation vats containing sugarcane through the determination of biochemical and molecular parameters associated with flocculation phenotypes. by analyzing thirteen cachaça yeast strains isolated from different distilleries, our results demons ... | 2014 | 25209588 |
| isolation of oleaginous yeast (rhodosporidium toruloides) mutants tolerant of sugarcane bagasse hydrolysate. | rhodosporidium toruloides is a lipid-producing yeast, the growth of which is severely suppressed when hydrolysates of lignocellulosic biomass are used as carbon source. this is probably due to the toxic substances, such as organic acids, furans, and phenolic compounds produced during the preparation of the hydrolysates. in order to solve this problem, r. toruloides cultures were subjected to atmospheric room-temperature plasma mutagenesis, resulting in the isolation of mutants showing tolerance ... | 2014 | 25036690 |
| multi-scale structural and chemical analysis of sugarcane bagasse in the process of sequential acid-base pretreatment and ethanol production by scheffersomyces shehatae and saccharomyces cerevisiae. | heavy usage of gasoline, burgeoning fuel prices, and environmental issues have paved the way for the exploration of cellulosic ethanol. cellulosic ethanol production technologies are emerging and require continued technological advancements. one of the most challenging issues is the pretreatment of lignocellulosic biomass for the desired sugars yields after enzymatic hydrolysis. we hypothesized that consecutive dilute sulfuric acid-dilute sodium hydroxide pretreatment would overcome the native r ... | 2014 | 24739736 |
| evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of pichia stipitis and its respiratory deficient. | the use of lignocellulosic residues for ethanol production is limited by toxic compounds in fermenting yeasts present in diluted acid hydrolysates like acetic acid and 2-furaldehyde. the respiratory deficient phenotype gives the cell the ability to resist several toxic compounds. so the aim of this work was to evaluate the tolerance to toxic compounds present in lignocellulosic hydrolysates like acetic acid and 2-furaldehyde in pichia stipitis and its respiratory deficient strains. the respirato ... | 2014 | 24700134 |
| impact of nano-caco3 -ldpe packaging on quality of fresh-cut sugarcane. | in order to evaluate the effects of nano-caco3 -based low density polyethylene (nano-caco3 -ldpe) packaging on the quality of fresh-cut sugarcane, concentrations of o2 and co2 within the packages, overall visual quality (ovq), total bacterial count (tbc), yeast and mould count (ymc), reducing sugar content and total phenolic content, respiration, ethylene production, and the activities of phenylalanine ammonia-lyase (pal), polyphenol oxidase (ppo), peroxidase (pod), acid invertase (ai) and neutr ... | 2014 | 24700113 |
| pseudozyma brasiliensis sp. nov., a xylanolytic, ustilaginomycetous yeast species isolated from an insect pest of sugarcane roots. | a novel ustilaginomycetous yeast isolated from the intestinal tract of an insect pest of sugarcane roots in ribeirão preto, são paulo state, brazil, represents a novel species of the genus pseudozyma based on molecular analyses of the d1/d2 rdna large subunit and the internal transcribed spacer (its1+its2) regions. the name pseudozyma brasiliensis sp. nov. is proposed for this species, with ghg001(t) ( = cbs 13268(t) = ufmg-cm-y307(t)) as the type strain. p. brasiliensis sp. nov. is a sister spe ... | 2014 | 24682702 |
| ethanol fermentation from molasses at high temperature by thermotolerant yeast kluyveromyces sp. iipe453 and energy assessment for recovery. | high temperature ethanol fermentation from sugarcane molasses b using thermophilic crabtree-positive yeast kluyveromyces sp. iipe453 was carried out in batch bioreactor system. strain was found to have a maximum specific ethanol productivity of 0.688 g/g/h with 92 % theoretical ethanol yield. aeration and initial sugar concentration were tuning parameters to regulate metabolic pathways of the strain for either cell mass or higher ethanol production during growth with an optimum sugar to cell rat ... | 2014 | 24682264 |
| selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast rhodosporidium toruloides ncyc 921 using flow cytometry. | the present work studied low-cost carbon sources for carotenoid and lipid production using the yeast rhodosporidum toruloides ncyc 921. carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in r. toruloides batch cultivations. carob pulp syrup containing a total sugar concentration of 75 g l(-1) induced the highest total fatty acid productivity (1.90 g l(-1)h(-1)) and the highest carotenoid productivity (9.79 μg l(-1)h(-1)). flow cytometric anal ... | 2014 | 24650616 |
| effect of feeding strategies on lipid production by lipomyces starkeyi. | the aim of this study was to produce microbial oil from lipomyces starkeyi dsm 70296 grown in hemicellulose hydrolysate (h-h). glucose and xylose were used for batch, fed-batch, repeated fed-batch, and continuous cultures, and h-h was tested at continuous culture. the highest cell and lipid concentrations of 85.4 and 41.8g/l, respectively, were obtained using repeated fed-batch strategy. continuous culture with dilution rate of 0.03h(-1) presented the highest overall cell (0.443g/g) and lipid yi ... | 2014 | 24556374 |
| solving ethanol production problems with genetically modified yeast strains. | the current world demand for bioethanol is increasing as a consequence of low fossil fuel availability and a growing number of ethanol/gasoline flex-fuel cars. in addition, countries in several parts of the world have agreed to reduce carbon dioxide emissions, and the use of ethanol as a fuel (which produces fewer pollutants than petroleum products) has been considered to be a good alternative to petroleum products. the ethanol that is produced in brazil from the first-generation process is opti ... | 2013 | 24516432 |
| exploration of potential baker's yeast from sugarcane juice: optimization and evaluation. | the present study was carried out to explore baker's yeasts strains from sugarcane juice to assess its potential in laboratory scale production of breads. collected juice samples were processed for isolation and identification of yeast strains based on standard cultural, morphological and biochemical characteristics. among the six isolated strains, four (designated as s1, s2, s5 and s6) were identified as saccharomyces cerevisiae and the rests (designated s3 and s4) were as s. rouxii. when asses ... | 2013 | 24505984 |
| an organic acid-tolerant haa1-overexpression mutant of an industrial bioethanol strain of saccharomyces cerevisiae and its application to the production of bioethanol from sugarcane molasses. | bacterial contamination is known as a major cause of the reduction in ethanol yield during bioethanol production by saccharomyces cerevisiae. acetate is an effective agent for the prevention of bacterial contamination, but it negatively affects the fermentation ability of s. cerevisiae. we have proposed that the combined use of organic acids including acetate and lactate and yeast strains tolerant to organic acids may be effective for the elimination of principally lactic acid bacterial (lab) co ... | 2013 | 24373204 |
| statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast rhodotorula sp. iip-33 using response surface methodology. | single cell oil production from sugarcane bagasse hydrolysate by oleaginous yeast rhodotorula sp. iip-33 was analyzed using a two stage statistical design approach based on response surface methodology. variables like pentose sugar, (nh4)2so4, kh2po4, yeast extract, ph and temperature were found to influence lipid production significantly. under optimized condition in a shake flask, yield of lipid was 2.1199 g with fat coefficient of 7.09 which also resembled ~99% similarity to model predicted l ... | 2014 | 26034686 |
| new cultive medium for bioconversion of c5 fraction from sugarcane bagasse using rice bran extract. | the use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. however, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioprocesses. this paper evaluates the fermentative performance of scheffersomyces (pichia) stipitis and candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with differe ... | 2014 | 25763056 |
| the fermentation of sugarcane molasses by dekkera bruxellensis and the mobilization of reserve carbohydrates. | the yeast dekkera bruxellensis is considered to be very well adapted to industrial environments, in brazil, usa, canada and european countries, when different substrates are used in alcoholic fermentations. our previous study described its fermentative profile with a sugarcane juice substrate. in this study, we have extended its physiological evaluation to fermentation situations by using sugarcane molasses as a substrate to replicate industrial working conditions. the results have confirmed the ... | 2014 | 24370978 |
| comparative genomic analysis of saccharomyces cerevisiae yeasts isolated from fermentations of traditional beverages unveils different adaptive strategies. | saccharomyces cerevisiae strains are the main responsible of most traditional alcohol fermentation processes performed around the world. the characteristics of the diverse traditional fermentations are very different according to their sugar composition, temperature, ph or nitrogen sources. during the adaptation of yeasts to these new environments provided by human activity, their different compositions likely imposed selective pressures that shaped the s. cerevisiae genome. in the present work ... | 2014 | 24334254 |
| brewer's yeast and sugarcane yeast as protein sources for dogs. | brewer's yeast (by), autolysed sugarcane yeast (ascy) and integral sugar cane yeast (iscy) were studied in two experiments as ingredients for dog diets. in the first experiment, 28 dogs were randomly assigned to four diets; one reference diet and three test diets containing 15% of by, ascy or iscy and 85% of the reference diet (as-fed basis). the digestibilities of the yeasts were calculated by the substitution method. in the second experiment, 35 dogs were randomized to five diets with similar ... | 2014 | 24304448 |
| exploring grape marc as trove for new thermotolerant and inhibitor-tolerant saccharomyces cerevisiae strains for second-generation bioethanol production. | robust yeasts with high inhibitor, temperature, and osmotic tolerance remain a crucial requirement for the sustainable production of lignocellulosic bioethanol. these stress factors are known to severely hinder culture growth and fermentation performance. | 2013 | 24286305 |
| design and optimization of a process for sugarcane molasses fermentation by saccharomyces cerevisiae using response surface methodology. | a statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolated saccharomyces cerevisiae y-39. response surface methodology rsm based on central composite face centered design ccfd was employed to statistically evaluate and optimize the conditions for maximum bioethanol production and study the significance and interaction of incubation period, initial ph, incubation temperature, and molasses concent ... | 2013 | 24222769 |
| lindane degradation by candida vitjzn04, a newly isolated yeast strain from contaminated soil: kinetic study, enzyme analysis and biodegradation pathway. | a new yeast strain was isolated from sugarcane cultivation field which was able to utilize lindane as sole carbon source for growth in mineral medium. the yeast was identified and named as candida sp. vitjzn04 based on a polyphasic approach using morphological, biochemical and 18s rdna, d1/d2 and its sequence analysis. the isolated yeast strain efficiently degraded 600 mg l⁻¹ of lindane within 6 days in mineral medium under the optimal conditions (ph 7; temperature 30 °c and inoculum dosage 0.06 ... | 2014 | 24217897 |
| continuous ethanol production from sugarcane molasses using a newly designed combined bioreactor system by immobilized saccharomyces cerevisiae. | continuous ethanol fermentation using polyvinyl alcohol (pva), immobilized yeast, and sugarcane molasses (22 and 35°bx) with 8 g/l urea was run in a combined bioreactor system consisting of three-stage tubular bioreactors in series. the effect of the dilution rate (d) at 0.0037, 0.0075, 0.0117, 0.0145, 0.018, and 0.0282 h(-1) on continuous ethanol fermentation was investigated in this study. the results showed that d had a significant effect on fermentation efficiency, sugar-utilized rate, ethan ... | 2014 | 24164318 |
| sugarcane vinasse: environmental implications of its use. | the inadequate and indiscriminate disposal of sugarcane vinasse in soils and water bodies has received much attention since decades ago, due to environmental problems associated to this practice. vinasse is the final by-product of the biomass distillation, mainly for the production of ethanol, from sugar crops (beet and sugarcane), starch crops (corn, wheat, rice, and cassava), or cellulosic material (harvesting crop residues, sugarcane bagasse, and wood). because of the large quantities of vina ... | 2013 | 24084103 |
| metabolic engineering of saccharomyces cerevisiae for increased bioconversion of lignocellulose to ethanol. | the absence of pentose-utilizing enzymes in saccharomyces cerevisiae is an obstacle for efficiently converting lignocellulosic materials to ethanol. in the present study, the genes coding xylose reductase (xyl1) and xylitol dehydrogenase (xyl2) from pichia stipitis were successfully engineered into s. cerevisae. as compared to the control transformant, engineering of xyl1 and xyl2 into yeasts significantly increased the microbial biomass (8.1 vs. 3.4 g/l), xylose consumption rate (0.15 vs. 0.02 ... | 2012 | 23997337 |
| a simple and effective set of pcr-based molecular markers for the monitoring of the saccharomyces cerevisiae cell population during bioethanol fermentation. | one of the defining features of the fermentation process used in the production of bioethanol from sugarcane feedstock is the dynamic nature of the yeast population. minisatellite molecular markers are particularly useful for monitoring yeast communities because they produce polymorphic pcr products that typically display wide size variations. we compared the coding sequences derived from the genome of the sugarcane bioethanol strain jay270/pe-2 to those of the reference saccharomyces cerevisiae ... | 2013 | 23994268 |
| physiology and gene expression profiles of dekkera bruxellensis in response to carbon and nitrogen availability. | the assimilation of nitrate, a nitrogenous compound, was previously described as an important factor favoring dekkera bruxellensis in the competition with saccharomyces cerevisiae for the industrial sugarcane substrate. in this substrate, nitrogen sources are limited and diverse, and a recent report showed that amino acids enable d. bruxellensis to grow anaerobically. thus, understanding the regulation of nitrogen metabolism is one fundamental aspect to comprehend the competiveness of d. bruxell ... | 2013 | 23959165 |
| conversion of c6 and c5 sugars in undetoxified wet exploded bagasse hydrolysates using scheffersomyces (pichia) stipitis cbs6054. | sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. this stresses the importance of utilizing both c6 and c5 sugars for conversion into ethanol in order to improve the process economics. during processing of the hydrolysate degradation products such as acetate, 5-hydroxymethylfurfural (hmf) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. in the current study, conversion of both glucose and xyl ... | 2013 | 23895663 |
| pseudozyma vetiver sp. nov., a novel anamorphic ustilaginomycetous yeast species isolated from the phylloplane in thailand. | three strains representing one novel yeast species were isolated from the phylloplanes of the vetiver grasses (dmku-lv90 and dmku-lv99(t)) and sugarcane (dmku-sp260) collected in thailand by leaf washing followed by a plating technique. on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and the sequence analysis of the d1/d2 region of the large subunit (lsu) rrna gene and the internal transcribed spacer region (its), the three strains were found to repre ... | 2013 | 23877892 |
| production of bioethanol from fermented sugars of sugarcane bagasse produced by lignocellulolytic enzymes of exiguobacterium sp. vsg-1. | exiguobacterium sp. vsg-1 was isolated from the soil sample and characterized for the production of lignocellulolytic enzymes. production of these enzymes by the strain vsg-1 was carried out using steam-exploded sugarcane bagasse (scb) and found to secrete cellulase, pectinase, mannanase, xylanase, and tannase. the growth and enzyme production were found to be optimum at ph 9.0 and 37 °c. upon steam explosion of scb, the cellulose increased by 42 %, whereas hemicelluloses and lignin decreased by ... | 2013 | 23832861 |
| rim15p-mediated regulation of sucrose utilization during molasses fermentation using saccharomyces cerevisiae strain pe-2. | inherited loss-of-function mutations in the rim15p-mediated stress-response pathway contribute to the high fermentation rate of sake yeast strains. in the present study, we found that disruption of the rim15 gene in ethanol-producing saccharomyces cerevisiae strain pe-2 accelerated molasses fermentation through enhanced sucrose utilization following glucose starvation. | 2013 | 23757382 |
| design and optimization of ethanol production from bagasse pith hydrolysate by a thermotolerant yeast kluyveromyces sp. iipe453 using response surface methodology. | ethanol production from sugarcane bagasse pith hydrolysate by thermotolerant yeast kluyveromyces sp. iipe453 was analyzed using response surface methodology. variables such as substrate concentration, ph, fermentation time and na2hpo4 concentration were found to influence ethanol production significantly. in a batch fermentation, optimization of key process variables resulted in maximum ethanol concentration of 17.44 g/l which was 88% of the theoretical with specific productivity of 0.36 g/l/h. | 2013 | 23710425 |
| wickerhamiella slavikovae sp. nov. and wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates. | two novel yeast species were isolated during three independent studies of yeasts associated with natural substrates in brazil and taiwan. analysis of the sequences of the d1/d2 domains of the large subunit rrna gene showed that these novel species belong to the wickerhamiella clade. the first was isolated from freshwater and a leaf of sugar cane (saccharum officinarum) in brazil and from leaves of wedelia biflora in taiwan. described here as wickerhamiella slavikovae sp. nov., it differs by 56 n ... | 2013 | 23710055 |
| ultrasonic pretreatment and acid hydrolysis of sugarcane bagasse for succinic acid production using actinobacillus succinogenes. | immense interest has been devoted to the production of bulk chemicals from lignocellulose biomass. diluted sulfuric acid treatment is currently one of the main pretreatment methods. however, the low total sugar concentration obtained via such pretreatment limits industrial fermentation systems that use lignocellulosic hydrolysate. sugarcane bagasse hemicellulose hydrolysate is used as the carbon and nitrogen sources to achieve a green and economical production of succinic acid in this study. sug ... | 2013 | 23649828 |
| detoxification of lignocellulosic hydrolysates using sodium borohydride. | addition of sodium borohydride to a lignocellulose hydrolysate of norway spruce affected the fermentability when cellulosic ethanol was produced using saccharomyces cerevisiae. treatment of the hydrolysate with borohydride improved the ethanol yield on consumed sugar from 0.09 to 0.31 g/g, the balanced ethanol yield from 0.02 to 0.30 g/g, and the ethanol productivity from 0.05 to 0.57 g/(l×h). treatment of a sugarcane bagasse hydrolysate gave similar results, and the experiments indicate that so ... | 2013 | 23567704 |
| in situ microscopy: a perspective for industrial bioethanol production monitoring. | this work reviews the state-of-the-art in image-based in situ methods with regard to their potential use for fermentation of saccharomyces cerevisiae in sugarcane wine. the integration of real time information from fermentation tanks in the control strategies has high potential to promote better fermentative performance. while several image-based techniques for the measurement of cell concentration have been established, a reliable and consistent viability measurement still remains a challenging ... | 2013 | 23524154 |
| the influence of nitrate on the physiology of the yeast dekkera bruxellensis grown under oxygen limitation. | a previous study showed that the use of nitrate by dekkera bruxellensis might be an advantageous trait when ammonium is limited in sugarcane substrate for ethanol fermentation. the aim of the present work was to evaluate the influence of nitrate on the yeast physiology during cell growth in different carbon sources under oxygen limitation. if nitrate was the sole source of nitrogen, d. bruxellensis cells presented slower growth, diminished sugar consumption and growth-associated ethanol producti ... | 2013 | 23440690 |
| stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation. | efforts were made to utilize the waste/by-product of two agro-process industries namely pressmud from sugar processing industries and spent yeast from distilleries manufacturing ethanol from cane molasses, for the production of microbial invertase. our experimentation indicated that these two sources could be ideally utilized for the production of invertase through solid substrate fermentation (ssf). ssf with spent yeast had given highest specific activity of 430 u/mg in 72 h of fermentation. in ... | 2012 | 23420549 |
| repeated batch cell-immobilized system for the biotechnological production of xylitol as a renewable green sweetener. | the present paper studies the biotechnological production of xylitol using sugarcane bagasse hydrolysate in a repeated batch fermentation system with immobilized cells of candida guilliermondii fti20037. immobilized cell system is considered as an attractive alternative to reuse the well-grown and adapted yeast cells in a new fresh fermentation media, without the need of the inoculum stage. in this work, seven repeated batches were performed in a fluidized bed bioreactor using immobilized cells ... | 2013 | 23397324 |
| transcriptional profiling of brazilian saccharomyces cerevisiae strains selected for semi-continuous fermentation of sugarcane must. | brazil played a pioneering role in the global establishment of the sugarcane bioethanol industry. the bioethanol fermentation process currently used in brazil is unique due to the acid wash and recycling of yeast cells. two, industrially adopted, wild yeast strains, cat-1 and pe-2, have become the most widely used in brazil. how these strains respond to the unique fermentation process is poorly understood. the improved performance of cat-1 and pe-2 is hypothesised to be related to enhanced stres ... | 2013 | 23360418 |
| lime pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse. | sugarcane bagasse was subjected to lime (calcium hydroxide) pretreatment and enzymatic hydrolysis for second-generation ethanol production. a central composite factorial design was performed to determine the best combination of pretreatment time, temperature, and lime loading, as well as to evaluate the influence of enzymatic loadings on hydrolysis conversion. the influence of increasing solids loading in the pretreatment and enzymatic hydrolysis stages was also determined. the hydrolysate was f ... | 2013 | 23334836 |
| ultra-structural mapping of sugarcane bagasse after oxalic acid fiber expansion (oafex) and ethanol production by candida shehatae and saccharomyces cerevisiae. | diminishing supplies of fossil fuels and oil spills are rousing to explore the alternative sources of energy that can be produced from non-food/feed-based substrates. due to its abundance, sugarcane bagasse (sb) could be a model substrate for the second-generation biofuel cellulosic ethanol. however, the efficient bioconversion of sb remains a challenge for the commercial production of cellulosic ethanol. we hypothesized that oxalic-acid-mediated thermochemical pretreatment (oafex) would overcom ... | 2013 | 23324164 |
| evaluation of novel xylose-fermenting yeast strains from brazilian forests for hemicellulosic ethanol production from sugarcane bagasse. | bioconversion of hemicellulosic hydrolysates into ethanol with the desired yields plays a pivotal role for the overall success of biorefineries. this paper aims to evaluate the ethanol production potential of four native strains of scheffersomyces shehatae (syn. candida shehatae) viz. s. shehatae br6-2ai, cg8-8by, pt1-1basp and br6-2ay, isolated from brazilian forests. these strains were grown in commercial d-xylose-supplemented synthetic medium and sugarcane bagasse hemicellulose hydrolysate. s ... | 2013 | 28324336 |
| rice bran extract: an inexpensive nitrogen source for the production of 2g ethanol from sugarcane bagasse hydrolysate. | selection of the raw material and its efficient utilization are the critical factors in economization of second generation (2g) ethanol production. fermentation of the released sugars into ethanol by a suitable ethanol producing microorganism using cheap media ingredients is the cornerstone of the overall process. this study evaluated the potential of rice bran extract (rbe) as a cheap nitrogen source for the production of 2g ethanol by scheffersomyces (pichia) stipitis nrrl y-7124 using sugarca ... | 2013 | 28324330 |
| improvement of ethanol production by ethanol-tolerant saccharomyces cerevisiae uvnr56. | ethanol tolerance is one of the important characteristics of ethanol-producing yeast. this study focused on the improvement of ethanol tolerance of saccharomyces cerevisiae nr1 for enhancing ethanol production by random uv-c mutagenesis. one ethanol-tolerant mutant, uvnr56, displayed a significantly improved ethanol tolerance in the presence of 15% (v/v) ethanol and showed a considerably higher viability during ethanol fermentation from sugarcane molasses and sugarcane molasses with initial etha ... | 2013 | 25674412 |
| what do we know about the yeast strains from the brazilian fuel ethanol industry? | the production of fuel ethanol from sugarcane-based raw materials in brazil is a successful example of a large-scale bioprocess that delivers an advanced biofuel at competitive prices and low environmental impact. two to three fed-batch fermentations per day, with acid treatment of the yeast cream between consecutive cycles, during 6-8 months of uninterrupted production in a nonaseptic environment are some of the features that make the brazilian process quite peculiar. along the past decades, so ... | 2013 | 23271669 |
| use of different extracts of coffee pulp for the production of bioethanol. | coffee is one of the most important agricultural products in brazil. more than 50 % of the coffee fruit is not used for the production of commercial green coffee and is therefore discarded, usually ending up in the environment. the goal of this work was to select an efficient process for obtaining coffee pulp extract and to evaluate the use of this extract in bioethanol production. the effects of heat treatment and trituration on the yield and composition of the extract were investigated by meas ... | 2013 | 23269634 |
| yamadazyma siamensis sp. nov., yamadazyma phyllophila sp. nov. and yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in thailand and taiwan. | four strains representing three novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (dmku-rk254(t)), corn leaves (dmku-rk548(t)), bean leaves (k129) in thailand and hengchun pencilwood leaves (trb1-1(t)) in taiwan. on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the d1/d2 region of the large subunit (lsu) rrna gene, the internal transcribed spacer (its) region, the actin gene (act1) and ... | 2013 | 23208204 |
| increase in ethanol production from sugarcane bagasse based on combined pretreatments and fed-batch enzymatic hydrolysis. | enzymatic hydrolysis of pretreated sugarcane bagasse was performed to investigate the production of ethanol. the sugarcane bagasse was pretreated in a process combining steam explosion and alkaline delignification. the lignin content decreased to 83%. fed-batch enzymatic hydrolyses was initiated with 8% (w/v) solids loading, and 10 fpu/g cellulose. then, 1% solids were fed at 12, 24 or 48 h intervals. after 120 h, the hydrolysates were fermented with saccharomyces cerevisiae ufpeda 1238, and a f ... | 2013 | 23201527 |
| an alkaline thermostable recombinant humicola grisea var. thermoidea cellobiohydrolase presents bifunctional (endo/exoglucanase) activity on cellulosic substrates. | humicola grisea var. thermoidea is a deuteromycete which secretes a large spectrum of hydrolytic enzymes when grown on lignocellulosic residues. this study focused on the heterologous expression and recombinant enzyme analysis of the major secreted cellulase when the fungus is grown on sugarcane bagasse as the sole carbon source. cellobiohydrolase 1.2 (cbh 1.2) cdna was cloned in pichia pastoris under control of the aox1 promoter. recombinant protein (rcbh1.2) was efficiently produced and secret ... | 2013 | 23054694 |
| genetic characterization and construction of an auxotrophic strain of saccharomyces cerevisiae jp1, a brazilian industrial yeast strain for bioethanol production. | used for millennia to produce beverages and food, saccharomyces cerevisiae also became a workhorse in the production of biofuels, most notably bioethanol. yeast strains have acquired distinct characteristics that are the result of evolutionary adaptation to the stresses of industrial ethanol production. jp1 is a dominant industrial s. cerevisiae strain isolated from a sugarcane mill and is becoming increasingly popular for bioethanol production in brazil. in this work, we carried out the genetic ... | 2012 | 22892884 |
| acid black 48 dye biosorption using saccharomyces cerevisiae immobilized with treated sugarcane bagasse. | the textile industry consumes large quantities of water and chemicals, especially in dyeing and finishing processes. textile dye adsorption can be accomplished with natural or synthetic compounds. cell immobilization using biomaterials allows the reduction of toxicity and mechanical resistance and opens spaces within the matrix for cell growth. the use of natural materials, such as sugarcane bagasse, is promising due to the low costs involved. the aim of the present study was to evaluate the use ... | 2012 | 22864427 |
| the consequences of lactobacillus vini and dekkera bruxellensis as contaminants of the sugarcane-based ethanol fermentation. | this work describes the effects of the presence of the yeast dekkera bruxellensis and the bacterium lactobacillus vini on the industrial production of ethanol from sugarcane fermentation. both contaminants were quantified in industrial samples, and their presence was correlated to a decrease in ethanol concentration and accumulation of sugar. then, laboratory mixed-cell fermentations were carried out to evaluate the effects of these presumed contaminants on the viability of saccharomyces cerevis ... | 2012 | 22842986 |
| identification of amino acids important for substrate specificity in sucrose transporters using gene shuffling. | plant sucrose transporters (suts) are h(+)-coupled uptake transporters. type i and ii (suts) are phylogenetically related but have different substrate specificities. type i suts transport sucrose, maltose, and a wide range of natural and synthetic α- and β-glucosides. type ii suts are more selective for sucrose and maltose. here, we investigated the structural basis for this difference in substrate specificity. we used a novel gene shuffling method called synthetic template shuffling to introduc ... | 2012 | 22807445 |
| metschnikowia saccharicola sp. nov. and metschnikowia lopburiensis sp. nov., two novel yeast species isolated from phylloplane in thailand. | five strains representing two novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (dmku-rk16, dmku-rk24, dmku-rk198 and dmku-rk500(t)) and rice leaf (dmku-rk277(t)) by an enrichment technique. on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the d1/d2 domain of the large subunit (lsu) rrna gene and the internal transcribed spacer region, the five strains were assigned to be two novel spec ... | 2012 | 22791018 |
| study of sugarcane pieces as yeast supports for ethanol production from sugarcane juice and molasses using newly isolated yeast from toddy sap. | a repeated batch fermentation system was used to produce ethanol using saccharomyces cerevisiae strain (ncim 3640) immobilized on sugarcane (saccharum officinarum l.) pieces. for comparison free cells were also used to produce ethanol by repeated batch fermentation. scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of ... | 2012 | 22783132 |
| pretreatment of sugarcane bagasse with nh4oh-h2o2 and ionic liquid for efficient hydrolysis and bioethanol production. | an efficient pretreatment method using nh(4)oh-h(2)o(2) and ionic liquid (il) was developed for the recovery of cellulose from sugarcane bagasse (scb). the regenerated scb from the combined pretreatment exhibited significantly enhanced enzymatic digestibility with an efficiency of 91.4% after 12h of hydrolysis, which was 64% higher than the efficiency observed for the regenerated scb after the individual nh(4)oh-h(2)o(2) pretreatment. 1-allyl-3-methylimidazolium chloride ([amim]cl) dissolved the ... | 2012 | 22728201 |
| whole-genome sequencing of the efficient industrial fuel-ethanol fermentative saccharomyces cerevisiae strain cat-1. | the saccharomyces cerevisiae strains widely used for industrial fuel-ethanol production have been developed by selection, but their underlying beneficial genetic polymorphisms remain unknown. here, we report the draft whole-genome sequence of the s. cerevisiae strain cat-1, which is a dominant fuel-ethanol fermentative strain from the sugarcane industry in brazil. our results indicate that strain cat-1 is a highly heterozygous diploid yeast strain, and the ~12-mb genome of cat-1, when compared w ... | 2012 | 22562254 |
| characterization of laccase isoforms produced by pleurotus ostreatus in solid state fermentation of sugarcane bagasse. | laccases are oxidative enzymes linked to biological degradation of lignin. the aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of pleurotus ostreatus. the addition of yeast extract 5 g/l, copper sulfate 150 μm and ferulic acid 2 mm provided highest enzymatic activity (167 u/g) and zymograms i ... | 2012 | 22487128 |
| understanding physiological responses to pre-treatment inhibitors in ethanologenic fermentations. | alcohol-based liquid fuels feature significantly in the political and social agendas of many countries, seeking energy sustainability. it is certain that ethanol will be the entry point for many sustainable processes. conventional ethanol production using maize- and sugarcane-based carbohydrates with saccharomyces cerevisiae is well established, while lignocellulose-based processes are receiving growing interest despite posing greater technical and scientific challenges. a significant challenge ... | 2012 | 22331581 |
| high gravity fermentation of sugarcane molasses to produce ethanol: effect of nutrients. | fermentation efficiency of more than 85% was obtained by high gravity fermentation of 33-34°bx (spec. gravity ≈1.134) molasses medium with certain nutrients, instead of generally employed medium containing ≈16% (w/v) total sugar (spec. gravity ≈1.090) for ethanol fermentation in distilleries to get maximum 80-85% conversion. the fermenting yeast, saccharomyces, has varied capabilities, depending on the species and nutrition for fermenting the high solids medium. the fermentation period was reduc ... | 2010 | 22815577 |
| biosynthesis of invertase by saccharomyces cerevisiae with sugarcane molasses as substrate. | biosynthesis of invertase by saccharomyces cerevisiae 01k32 was inversely proportional to the concentration of sugarcane blackstrap molasses included in the medium. in a fermenter, an intracellular invertase activity of 440 u/g dry cells was obtained. | 1993 | 24420295 |
| production of α-galactosidase by monascus grown on soybean and sugarcane wastes. | extracts of both sugarcane and soybean wastes supported the growth of monascus but sugarcane waste was superior for the production of α-galactosidase. an aqueous extract prepared from 5% (w/v) soybean waste and 7% (w/v) sugarcane waste gave the best result and was superior to the standard peptone/glucose/yeast extract medium. liquid-solid mixtures were slightly less effective. enzyme production could be enhanced by adding raffinose. enzymatic hydrolysis of p-nitrophenyl-α-d-galactoside was optim ... | 1993 | 24420194 |
| cellulase production from a new strain acremonium strictum isolated from the brazilian biome using different substrates. | the objective of the present study was to evaluate the production of cellulolytic enzymes by acremonium strictum isolated from brazilian biome using different substrates. fermentations were initially carried out using commercial substrates, including microcrystalline cellulose (avicel® and servacel®) and carboxymethylcellulose (cmc). this was followed by fermentations performed using lignocellulosic biomass: sugarcane bagasse pretreated at different intensities. the fermentations were carried ou ... | 2013 | 23186655 |
| characterization of a sorghum mosaic virus (srmv) isolate in china. | sorghum mosaic virus (srmv), a causal agent of the destructive sugarcane mosaic disease, has a global presence. an isolate of srmv infecting a commercially-grown sugarcane plant was recovered from the hainan province of china. the virions were visualized by an electron microscope, and the coat proteins (cps) were sequenced by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and tandem mass spectrometry. discrepancies between the cp predicted and actual amino acid sequ ... | 2016 | 26981005 |
| a one-step real-time rt-pcr assay for the detection and quantitation of sugarcane streak mosaic virus. | sugarcane mosaic disease is caused by the sugarcane streak mosaic virus (scsmv; genus poacevirus, family potyviridae) which is common in some asian countries. here, we established a protocol of a one-step real-time quantitative reverse transcription pcr (real-time qrt-pcr) using the taqman probe for the detection of scsmv in sugarcane. primers and probes were designed within the conserved region of the scsmv coat protein (cp) gene sequences. standard single-stranded rna (ssrna) generated by pcr- ... | 2015 | 26185758 |
| transgenic sugarcane resistant to sorghum mosaic virus based on coat protein gene silencing by rna interference. | as one of the critical diseases of sugarcane, sugarcane mosaic disease can lead to serious decline in stalk yield and sucrose content. it is mainly caused by potyvirus sugarcane mosaic virus (scmv) and/or sorghum mosaic virus (srmv), with additional differences in viral strains. rna interference (rnai) is a novel strategy for producing viral resistant plants. in this study, based on multiple sequence alignment conducted on genomic sequences of different strains and isolates of srmv, the conserve ... | 2015 | 25685813 |
| development of a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for the detection of sugarcane mosaic virus and sorghum mosaic virus in sugarcane. | a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detecting sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) in sugarcane. six sets of four primers corresponding to the conserved coat protein gene were designed and tested for each virus. three primer sets designed for detecting scmv and four for detecting srmv were successful in the rt-lamp assay. the effective primer sets were not only specific for their target virus, but also able to ... | 2015 | 25445794 |
| inheritance and segregation of virus and herbicide resistance transgenes in sugarcane. | transgenic sugarcane parents containing multiple copies of herbicide resistance ( bar) and sorghum mosaic virus (srmv) resistance ( hut) genes were crossed with non-transgenic sugarcane varieties. segregation of the transgenes in the progeny was determined using southern blot analysis; herbicide resistance and srmv resistance were assessed using bioassays. the segregation data were used to infer linkage relationships between transgenes in the parent plants. in two of the parents, all transgene i ... | 2002 | 12582639 |
| phenolic content, antioxidant and antibacterial activity of selected natural sweeteners available on the polish market. | seventeen natural sweeteners available on the polish market were screened for total phenolic content, by the folin-ciocalteu method, and for antioxidant activity, using the ferric reducing antioxidant power (frap) assay and the 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) radical cation decolorization assay (abts(·+)). in addition, we analyzed antibacterial activities against staphylococcus aureus strains: both those susceptible and those resistant to methicillin (mrsa). the results of ... | 2013 | 24007486 |
| rapid and simple dna extraction method for the detection of enterotoxigenic staphylococcus aureus directly from food samples: comparison of pcr and lamp methods. | the study describes the development of simple and rapid dna extraction method in combination with loop-mediated isothermal amplification (lamp) to detect enterotoxigenic staphylococcus aureus in food samples. | 2012 | 22519925 |
| genome editing in sugarcane: challenges ahead. | genome editing opens new and unique opportunities for researchers to enhance crop production. until 2013, the zinc finger nucleases (zfns) and transcription activator-like effector nucleases (talens) were the key tools used for genome editing applications. the advent of rna-guided engineered nucleases - the type ii clustered regularly interspaced short palindromic repeat (crispr)/cas9 (crispr-associated) system from streptococcus pyogenes holds great potential since it is simple, effective and m ... | 2016 | 27790238 |
| expression analysis of hairpin rna carrying sugarcane mosaic virus (scmv) derived sequences and transgenic resistance development in a model rice plant. | developing transgenic resistance in monocotyledonous crops against pathogens remains a challenging area of research. sugarcane mosaic virus (scmv) is a serious pathogen of many monocotyledonous crops including sugarcane. the objective of present study was to analyze transgenic expression of hairpin rna (hprna), targeting simultaneously cp (coat protein) and hc-pro (helper component-proteinase) genes of scmv, in a model rice plant. conserved nucleotide sequences, exclusive for dag (aspartic acid- ... | 2017 | 28255554 |
| field performance of transgenic sugarcane lines resistant to sugarcane mosaic virus. | sugarcane mosaic disease is mainly caused by the sugarcane mosaic virus (scmv), which can significantly reduce stalk yield and sucrose content of sugarcane in the field. coat protein mediated protection (cpmp) is an effective strategy to improve virus resistance. a 2-year field study was conducted to compare five independent transgenic sugarcane lines carrying the scmv-cp gene (i.e., b2, b36, b38, b48, and b51) with the wild-type parental clone badila (wt). agronomic performance, resistance to s ... | 2017 | 28228765 |
| an atypical thioredoxin imparts early resistance to sugarcane mosaic virus in maize. | sugarcane mosaic virus (scmv) causes substantial losses of grain yield and forage biomass in susceptible maize worldwide. a major quantitative trait locus, scmv1, has been identified to impart strong resistance to scmv at the early infection stage. here, we demonstrate that zmtrxh, encoding an atypical h-type thioredoxin, is the causal gene at scmv1, and that its transcript abundance correlated strongly with maize resistance to scmv. zmtrxh alleles, whether they are resistant or susceptible, sha ... | 2017 | 28216424 |
| molecular variability and distribution of sugarcane mosaic virus in shanxi, china. | sugarcane mosaic virus (scmv) is responsible for large-scale economic losses in the global production of sugarcane, maize, sorghum, and some other graminaceous species. to understand the evolutionary mechanism of scmv populations, this virus was studied in shanxi, china. a total of 86 maize leaf samples (41 samples in 2012 and 45 samples in 2013) were collected from 4 regions of shanxi. | 2016 | 26987060 |
| the complete genome sequences of two naturally occurring recombinant isolates of sugarcane mosaic virus from iran. | sugarcane mosaic virus (scmv) is the most prevalent virus causing sugarcane mosaic and maize dwarf mosaic diseases. here, we presented the first two complete genomic sequences of iranian scmv isolates, nra and zra from sugarcane and maize. the complete genome sequences of nra and zra were, respectively, 9571 and 9572 nucleotides (nt) in length, excluding the 3'-terminal poly(a) tail. both isolates contained a 5'-untranslated region (utr) of 149 nt, an open reading frame of 9192 nt encoding a pol ... | 2016 | 26905544 |
| characterization of sugarcane mosaic virus scmv1 and scmv2 resistance regions by regional association analysis in maize. | sugarcane mosaic virus (scmv) causes one of the most severe virus diseases in maize worldwide, resulting in reduced grain and forage yield in susceptible cultivars. in this study, two association panels consisting of 94 inbred lines each, from china and the u.s., were characterized for resistance to two isolates: scmv-seehausen and scmv-bj. the population structure of both association panels was analyzed using 3072 single nucleotide polymorphism (snp) markers. the chinese and the u.s. panel were ... | 2015 | 26488483 |
| sugarcane elongin c is involved in infection by sugarcane mosaic disease pathogens. | sugarcane (saccharum sp. hybrid) provides the main source of sugar for humans. sugarcane mosaic disease (smd) is a major threat to sugarcane production. currently, control of smd is mainly dependent on breeding resistant cultivars through hybridization, which is time-consuming. understanding the mechanism of viral infection may facilitate novel strategies to breed cultivars resistant to smd and to control the disease. in this study, a wide interaction was detected between the viral vpg protein a ... | 2015 | 26362180 |
| phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus. | protein phosphorylation plays a pivotal role in the regulation of many cellular events. no information is yet available, however, on protein phosphorylation in plants in response to virus infection. in this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (zea mays l.) in response to sugarcane mosaic virus (scmv) infection. based on isotope tags for relative and absolute quantification technology, tio2 enrichment method and lc-ms/ms analysis, we identified ... | 2015 | 25488425 |
| characterization of small interfering rnas derived from sugarcane mosaic virus in infected maize plants by deep sequencing. | rna silencing is a conserved surveillance mechanism against viruses in plants. it is mediated by dicer-like (dcl) proteins producing small interfering rnas (sirnas), which guide specific argonaute (ago)-containing complexes to inactivate viral genomes and may promote the silencing of host mrnas. in this study, we obtained the profile of virus-derived sirnas (vsirnas) from sugarcane mosaic virus (scmv) in infected maize (zea mays l.) plants by deep sequencing. our data showed that vsirnas which d ... | 2014 | 24819114 |
| combined linkage and association mapping reveals candidates for scmv1, a major locus involved in resistance to sugarcane mosaic virus (scmv) in maize. | sugarcane mosaic virus (scmv) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. maize resistance to scmv is associated with two dominant genes, scmv1 and scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. we combined both linkage and association mapping to identify positional candidate genes for scmv1. | 2013 | 24134222 |
| proteomic and phytohormone analysis of the response of maize (zea mays l.) seedlings to sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. control strategies have been developed, but only marginal successes have been achieved. for the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required. | 2013 | 23894637 |
| comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is an important viral pathogen and has caused serious losses in grain and forage yield. to identify candidate scmv resistance proteins and to explore the molecular mechanisms involved in the plant-scmv interaction, we conducted proteomic analyses of leaf samples from resistant and susceptible ecotypes of maize infected with scmv. proteins were analyzed by quantitative two-dimensional differential gel electrophoresis (2d-dige), and 93 protein spots showed statistical ... | 2013 | 23770298 |
| genetic diversity and population structure of sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is one of the causal pathogens of mosaic diseases on sugarcane, maize, sorghum and some other graminaceous species with worldwide distribution. the global genetic diversity and molecular evolution of scmv capsid protein (cp) gene were investigated with the nucleotide sequences available in the genbank database. phylogenetic analyses revealed that scmv isolates clustered in relation to their original hosts, and geographically distinct isolates from maize or sugarcane ... | 2013 | 23116592 |
| genomic variability and molecular evolution of asian isolates of sugarcane streak mosaic virus. | sugarcane streak mosaic virus (scsmv), an economically important causal agent of mosaic disease of sugarcane, is a member of the newly created genus poacevirus in the family potyviridae. in this study, we report the molecular characterization of three new scsmv isolates from china (yn-yz211 and hn-yz49) and myanmar (mya-formosa) and their genetic variation and phylogenetic relationship to scsmv isolates from asia and the type members of the family potyviridae. the complete genome of each of the ... | 2016 | 26973230 |
| genetic structure of populations of sugarcane streak mosaic virus in china: comparison with the populations in india. | sugarcane streak mosaic virus (scsmv) causes mosaic and streak symptoms on sugarcane and sorghum crops, and has a broad host range. scsmv is a member of the genus poacevirus in the family potyviridae.ten scsmv isolates were collected from sugarcane plants showing mosaic and streaking in southern china from 2009-2011. sequence-based phylogenetic and population genetic analyses were conducted using four partial genomic sequences covering the full genomes. these analyses were used to estimate the s ... | 2016 | 26432446 |
| molecular characterization of indian sugarcane streak mosaic virus isolates reveals recombination and negative selection in the p1 gene. | sugarcane streak mosaic virus (scsmv), a member of the genus poacevirus is an important viral pathogen affecting sugarcane production in india. the p1 gene of ten indian isolates was sequenced and compared with previously reported scsmv isolates. comparative sequence analysis revealed a high level of diversity in the p1 gene (83-98% nucleotide sequence identity; 87-100% amino acid sequence identity), and the indian scsmv isolates were found to be the most variable (up to 9% diversity at the amin ... | 2014 | 25225126 |
| molecular variability of sugarcane streak mosaic virus in china based on an analysis of the p1 and cp protein coding regions. | sequences of the protein 1 (p1) and coat protein (cp) coding regions of 22 sugarcane streak mosaic virus (scsmv) isolates were determined. phylogenetic analysis showed that scsmv had at least three major lineages, and the lineages seemed to reflect geographical origin. the sudden expansions of the chinese and indian subpopulations were supported by calculations showing deviations from the neutral equilibrium model for the individual lineages with an overall lack of nucleotide diversity. our stud ... | 2014 | 24178307 |
| identification and validation of sugarcane streak mosaic virus-encoded micrornas and their targets in sugarcane. | plants have developed several defense mechanisms to cope with various pathogens (bacteria, fungi, virus, and phytoplasma). among these, rna interference (rnai)-mediated defense against viral infection was found to be a major innate immune response. as a counter attack strategy against the host defense, viruses produce suppressors of host rnai pathway. micrornas (mirnas) are an abundant class of short (~18-22 nucleotide) non-coding single-stranded rnas involved in rnai pathway leading to post-tra ... | 2014 | 24145912 |
| molecular characterization of indian sugarcane streak mosaic virus isolate. | sugarcane streak mosaic virus (scsmv), a member of the genus poacevirus, family potyviridae, is an important viral pathogen affecting sugarcane cultivation in india. the complete nucleotide sequence of a scsmv isolate from india (scsmv-ind) was determined. the linear, assembled, single-stranded positive-sense rna genome of scsmv-ind was 9,786 nucleotides in length (excluding the poly (a) tail) and encoded a polyprotein of 3,131 amino acid residues. the genome of scsmv-ind shared high degree of s ... | 2013 | 23011777 |
| genetic variability and potential recombination events in the hc-pro gene of sugarcane streak mosaic virus. | sugarcane streak mosaic virus (scsmv), a member of the family potyviridae, is an important viral pathogen affecting sugarcane production in india. the variability in the nucleotide (nt) and amino acid (aa) sequences of helper component proteinase (hc-pro) of scsmv isolates from india was investigated and compared with those of previously published virus isolates from different asian countries. comparison of all of the sequenced virus isolates revealed a high level of diversity in the hc-pro gene ... | 2012 | 22481599 |
| quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, melanaphis sacchari. | yellow leaf caused by sugarcane yellow leaf virus (scylv) is a serious constraint to sugarcane production in india and currently the disease epidemics occur on many of the susceptible varieties under field conditions. studies were conducted on the virus transmission by sugarcane aphid melanaphis sacchari in sugarcane by inoculating virus-free meristem derived from micro- propagated plants of sugarcane cv co 86032 with viruliferous aphids. virus transmission was confirmed through rt-pcr assays an ... | 2015 | 26645033 |
| biology and management of sugarcane yellow leaf virus: an historical overview. | sugarcane yellow leaf virus (scylv) is one of the most widespread viruses causing disease in sugarcane worldwide. the virus has been responsible for drastic economic losses in most sugarcane-growing regions and remains a major concern for sugarcane breeders. infection with scylv results in intense yellowing of the midrib, which extends to the leaf blade, followed by tissue necrosis from the leaf tip towards the leaf base. such symptomatic leaves are usually characterized by increased respiration ... | 2015 | 26424197 |
| molecular evolutionary history of sugarcane yellow leaf virus based on sequence analysis of rna-dependent rna polymerase and putative aphid transmission factor-coding genes. | rna-dependent rna polymerase (rdrp) encoded by orf2 and putative aphid transmission factor (patf) encoded by orf5 of sugarcane yellow leaf virus (scylv) were detected in six sugarcane cultivars affected by yellow leaf using rt-pcr and real-time rt-pcr assays. expression of both genes varied among infected plants, but overall expression of rdrp was higher than expression of patf. cultivar h87-4094 from hawaii yielded the highest transcript levels of rdrp, whereas cultivar c1051-73 from cuba exhib ... | 2014 | 24952671 |
| prospecting sugarcane resistance to sugarcane yellow leaf virus by genome-wide association. | using gwas approaches, we detected independent resistant markers in sugarcane towards a vectored virus disease. based on comparative genomics, several candidate genes potentially involved in virus/aphid/plant interactions were pinpointed. yellow leaf of sugarcane is an emerging viral disease whose causal agent is a polerovirus, the sugarcane yellow leaf virus (scylv) transmitted by aphids. to identify quantitative trait loci controlling resistance to yellow leaf which are of direct relevance for ... | 2014 | 24916990 |