| prevalance of chlamydia trochomatis, ureaplasma urealyticum and mycoplasma hominis infections in the unexplained infertile women. | to prospectively investigate the prevalence of chlamydia trachomatis (ct), mycoplasma hominis (mh) and ureaplasma urealyticum (uu) in the cervical canal and pouch of douglas in unexplained infertile women and compare it to healthy controls in the turkish population. | 2007 | 17160569 |
| biochemical and serological characterization of mycoplasma strains isolated from the genital tracts of humans in nigeria. | fifty-five (55) mycoplasma strains isolated from the genital tracts of humans were biochemically characterized using various biochemical tests and also serologically identified by growth inhibition technique using 5 mycoplasma antisera namely m. hominis pg2 1: m. genitalium g37: m. penetrans gtu54 and 2 strains of m. fermentans pg18 (hrc 6-62-s-170 and mb713-501-069). biochemically, 43 (78.2%) strains were identified as mycoplasma hominis, 8 (14.5%) strains as m. fermentans and 4 (7.3%) as m. pe ... | 2006 | 17209306 |
| effect of experimental genital mycoplasmosis on production of matrix metalloproteinases in membranes and amniotic fluid of sprague-dawley rats. | preterm, premature rupture of membranes (pprom) is a dire pregnancy outcome that is frequently associated with infection by the genital mycoplasmas, mycoplasma hominis, ureaplasma parvum, and u. urealyticum. one potential mechanism by which these microorganisms may cause pprom is by increasing the concentration of matrix metalloproteinases (mmps) in the membranes and amniotic fluid. we tested this hypothesis in a well-defined model system of genital infection with m. pulmonis, a natural reproduc ... | 2007 | 17217365 |
| [spontaneous urogenital infection in macaques]. | rates of natural infection of macaques with microorganisms that cause urogenital tract infections (ugt)--chlamydias, mycoplasms, ureaplasms and trichomonades--have been assessed. 198 macaques (rhesus, javan and lapunder) aged from newborn to 25 years (born healthy or from females with complicated pregnancy or labour) were examined. high rate of infection (40%) was observed in healthy macaques by pcr assay. in animals born from females with complicated pregnancy or labour (abortion, complicated l ... | 2006 | 17297890 |
| genital mycoplasmas in non-sexually active young females with vaginal discharge. | | 2007 | 17306803 |
| newest approaches to treatment of pelvic inflammatory disease: a review of recent randomized clinical trials. | treatment of pelvic inflammatory disease (pid) should provide high rates of clinical and microbiological cure for a range of pathogens and should ultimately prevent reproductive morbidity. between 1992 and 2006, 5 randomized clinical trials of moxifloxacin (1 trial), ofloxacin (1 trial), clindamycin-ciprofloxacin (1 trial), and azithromycin (2 trials) treatment among women with mild to moderate pid were found to have clinical cure rates of 90%-97%. trials of ofloxacin and clindamycin-ciprofloxac ... | 2007 | 17342647 |
| potential triggering infections of reactive arthritis. | the aim of the study was to investigate possible triggering infections causing reactive arthritis (rea) of urogenital origin. | 2006 | 17343254 |
| ureaplasma urealyticum and mycoplasma hominis infection in women with urogenital diseases. | the aim of the study was to assess the incidence of ureaplasma urealyticum (u. urealyticum) and mycoplasma hominis (m. hominis) infection in women with urogenital diseases. | 2006 | 17357319 |
| mycoplasma hominis and ureaplasma urealyticum infections in male urethritis and its complications. | the aim of this study was to estimate the incidence of m. hominis and u. urealyticum infections among men with urethritis and its complications. | 2006 | 17357320 |
| pcr versus culture in the detection of vaginal ureaplasma urealyticum and mycoplasma hominis. | | 2007 | 17368648 |
| genital infection in males with idiopathic infertility. | we investigated a group of male patients with idiopathic infertility to determine the presence of genital infection and to identify the pattern of this infection using a specially designed protocol. a prospective study was carried out on 63 patients and 23 controls. we cultured the first voided urine, semen and swabs taken from the anterior urethra of these patients and controls for bacteria, chlamydia. ureaplasma urealyticum and mycoplasma hominis. two techniques were used for chlamydial isolat ... | 1996 | 17372403 |
| the role of mycoplasmas in pregnancy outcome. | mycoplasma hominis was isolated from a bartholin's gland abscess 70 years ago, and ureaplasmas were isolated from the genital tract about 20 years later. subsequently, reports incriminating mycoplasmas in the known adverse outcomes of pregnancy have been legion. without doubt these genital mycoplasmas are able to invoke an inflammatory response and take part in the cascade of events that culminates in preterm birth. their role in this and other conditions is becoming clearer, but controversy rem ... | 2007 | 17374511 |
| prevalence and clinical significance of ureaplasma urealyticum and mycoplasma hominis in the lower genital tract of hiv-1-infected women. | mycoplasma hominis and ureaplasma urealyticum are frequently isolated from the cervical and vaginal tracts of hiv-negative asymptomatic women. published data lack indisputable conclusions and doubts still exist as to whether these mycoplasmas are pathogens or mere co-factors associated with genital infections. we therefore conducted a surveillance study to investigate the prevalence of genital mycoplasmas in hiv-1 positive women (110 patients), attending the outpatient infectious diseases clinic ... | 2006 | 17380088 |
| genital mycoplasmas--morbidity and a potential influence on human fertility. | mycoplasmas comprise a big group of organisms consisting of one hundred eighty species which are found in nature as parasites of humans, other mammals, reptiles, fishes and plants, or living as commensals. the group commonly referred to as genital mycoplasmas comprise species most often found in the genitourinary tract of sexually active adults as common commensal inhabitants, or pathogens which can cause many different infections. the species we are most interested in this work are called: myco ... | 2006 | 17401188 |
| antibiotic administration to patients with preterm premature rupture of membranes does not eradicate intra-amniotic infection. | antibiotic administration has become part of the standard of care for patients with preterm premature rupture of membranes (prom). yet, the natural history of intrauterine infection/inflammation during antibiotic therapy remains largely unknown. this study was conducted to determine if antibiotic administration to the mother eradicates intra-amniotic infection and/or reduces the frequency of intra-amniotic inflammation, a risk factor for impending preterm labor/delivery and adverse neonatal outc ... | 2007 | 17437216 |
| rapid and efficient preparation of monoclonal antibodies against 35 kda lipoprotein of mycoplasma penetrans. | to develop a rapid and efficient method for preparing monoclonal antibodies (mab) against 35 kda lipoprotein of mycoplasma penetrans, balb/c mice were injected into the footpads for immunization, and the popliteal lymph nodes were isolated 19 days later for mab-producing hybridomas, from which the mabs against the 35 kda lipoprotein were screened. the identification of the mab against the 35 kda lipoprotein was performed using indirect enzyme-linked immunosorbent assay (elisa) and immunoblotting ... | 2007 | 17451357 |
| treatment of men with urethritis negative for neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum and ureaplasma urealyticum. | some patients with symptomatic non-gonococcal urethritis (ngu) are negative for chlamydia trachomatis, mycoplasmas and ureaplasmas. the optimal antimicrobial chemotherapy for such ngu has not fully been elucidated, though many studies of antimicrobial chemotherapies for c. trachomatis-positive ngu have been performed. we assessed the efficacy of antimicrobial agents that are active against c. trachomatis on non-mycoplasmal, non-ureaplasmal and non-chlamydial ngu (nmnuncngu). | 2007 | 17511725 |
| organisms associated with bacterial vaginosis in nigerian women as determined by pcr-dgge and 16s rrna gene sequence. | bacterial vaginosis (bv) is a condition with diverse etiology. this condition predisposes women to increased susceptibility to sexually transmitted diseases, including human immunodeficiency virus (hiv) infections and preterm birth. the diagnostic methods currently adopted in the evaluation of patient samples for bv are arguably amsel criteria, and nugent score that require microscopy and expert interpretation. these two methods are still subjective. | 2007 | 17594282 |
| presence of mycoplasma and ureaplasma species in the vagina of women of reproductive age. | against the background of genital mycoplasmas being implicated as pathogens in the genital tracts of adult humans, this study was carried out to determine the prevalence of genital mycoplasmas among women of reproductive age attending a tertiary-care hospital in nigeria. | 2007 | 17595988 |
| [the bacterial vaginosis--treatment problems]. | the changes in vaginal ecosystem (decreasing number of h2o2 producing lactobacillus spp. and increasing number of mobiluncus spp., gardnerella vaginalis, atopobium vaginae, prevotella spp., mycoplasma hominis, peptostreptococcus spp.) appears to be a major cause of bacterial vaginosis (bv). bacterial vaginosis is associated with infectious complications in pregnant and non-pregnant women and increasing risk of sti. the preventive role of natural vaginal microflora and problems of bv treatment ar ... | 2007 | 17607971 |
| "candidatus bacilloplasma," a novel lineage of mollicutes associated with the hindgut wall of the terrestrial isopod porcellio scaber (crustacea: isopoda). | pointed, rod-shaped bacteria colonizing the cuticular surface of the hindgut of the terrestrial isopod crustacean porcellio scaber (crustacea: isopoda) were investigated by comparative 16s rrna gene sequence analysis and electron microscopy. the results of phylogenetic analysis, and the absence of a cell wall, affiliated these bacteria with the class mollicutes, within which they represent a novel and deeply branched lineage, sharing less than 82.6% sequence similarity to known mollicutes. the l ... | 2007 | 17630315 |
| evaluation of the mycoplasma duo kit for the detection of mycoplasma hominis and ureaplasma urealyticum from urogenital and placental specimens. | this study compares the mycoplasma duo kit for the detection of genital mycoplasmas with conventional culture using a7 differential agar for the detection of mycoplasma hominis and ureaplasma urealyticum in clinical samples. detection of the mycoplasmas is based on the specific metabolic properties of each organism to hydrolyse either arginine or urea. the mycoplasma duo test showed a significantly higher detection rate than did culture, although many of the culture-negative results may have bee ... | 2007 | 17633140 |
| the alabama preterm birth study: diffuse decidual leukocytoclastic necrosis of the decidua basalis, a placental lesion associated with preeclampsia, indicated preterm birth and decreased fetal growth. | laminar necrosis, a band-like distribution of coagulative necrosis, has been reported at the choriodecidual interface of the free membranes of placentas of women with various adverse neonatal outcomes. our goal in this study was to evaluate the frequency of an equivalent feature in the decidua basalis, diffuse decidual leukocytoclastic necrosis (ddln), a diffuse coagulative necrosis admixed with karyorrhectic debris, in preterm births <32 weeks, and to determine its association with various obst ... | 2007 | 17674243 |
| entry and intracellular location of mycoplasma hominis in trichomonas vaginalis. | the parasite trichomonas vaginalis causes one of the most common non-viral sexually transmitted infections in humans. the coexistence of different sexually transmitted diseases in the same individual is very common, such as vaginal infections by t. vaginalis in association with mycoplasma fermentans or mycoplasma hominis. however, the consequences and behavior of mycoplasma during trichomonad infections are virtually unknown. this study was undertaken to elucidate whether mycoplasmas enter and l ... | 2008 | 17710384 |
| [analysis of detection and antimicrobial resistance of pathogens in prostatic secretion from 1186 infertile men with chronic prostatitis]. | to investigate the distribution and the antimicrobial resistance of the bacteria, mycoplasma and chlamydia trachomatis isolated from the prostatic secretion of infertile men with chronic prostatitis, and to provide clinicians with grounds for choosing antibiotic agents. | 2007 | 17725308 |
| [special features of chronic cystitis with leukoplasional changes of a mucous environment women's urinary bladder]. | | 2007 | 17726981 |
| the role of mycoplasma genitalium and ureaplasma urealyticum biovar 2 in postgonococcal urethritis. | there are few studies on coinfection with genital mycoplasmas and ureaplasmas in men with gonococcal urethritis (gu). the role of these species in postgonococcal urethritis (pgu) is poorly understood. thus, we conducted a study to determine the prevalence of coinfection with genital mycoplasmas and ureaplasmas among men with gu and to assess the role of these pathogens in pgu. | 2007 | 17806051 |
| ureaplasma urealyticum and mycoplasma hominis susceptibility to antimicrobial agents. | | 2007 | 17855195 |
| a case of mycoplasma hominis septic arthritis postpartum. | a 17-year-old woman presented 1-week post caesarean section with septic arthritis which was unresponsive to first-line empirical antimicrobial therapy. cultures of the hip aspirate revealed mycoplasma hominis as the causative organism. m. hominis-associated septic arthritis is extremely rare in the absence of underlying joint abnormalities or immunosuppression. strikingly four of the 26 cases reported have been pregnancy-associated. | 2007 | 17892899 |
| [mycoplasmas in non-gonococcal urethritis]. | the paper presents data on the etiological role of different types of genital mycoplasmas, such as u. urealyticum (u), m. hominis (mh), m. genitalium (mg), in the development of non-gonococcal urethritis (ngu). a hundred and twenty patients with acute and chronic urethritis were examined. a control group comprised clinically healthy males. polymerase chain reaction and cultural tests more frequently revealed genital mycoplasmas in patients with acute and chronic urethritis than in the controls, ... | 2007 | 17915486 |
| [frequency of detection of ureaplasma urealyticum and mycoplasma hominis in cervical canal and the douglas pouch of infertile and fertile women]. | the group of organisms commonly referred to as genital mycoplasmas comprise species most often found in genitourinary tract of sexually active adults as common commensal inhabitants, or pathogens which can possibly cause many different pathologies like: non-gonococcal urethritis, bacterial vaginosis, cervicitis, endometritis or pelvic inflammatory disease. the problem of their morbidity and the possible influence they have on human fertility is still not clear. the aim of this study was to find ... | 2007 | 17929414 |
| [mycoplasma pericarditis]. | | 2007 | 17953036 |
| ureaplasma urealyticum, ureaplasma parvum, mycoplasma hominis and mycoplasma genitalium infections and semen quality of infertile men. | genital ureaplasmas (ureaplasma urealyticum and ureaplasma parvum) and mycoplasmas (mycoplasma genitalium and mycoplasma hominis) are potentially pathogenic species playing an etiologic role in both genital infections and male infertility. reports are, however, controversial regarding the effects of these microorganisms infections in the sperm seminological variables. this study aimed at determining the frequency of genital ureplasmas and mycoplasmas in semen specimens collected from infertile m ... | 2007 | 17988404 |
| tetracycline resistance in ureaplasma spp. and mycoplasma hominis: prevalence in bordeaux, france, from 1999 to 2002 and description of two tet(m)-positive isolates of m. hominis susceptible to tetracyclines. | twenty-four of 128 clinical isolates of mycoplasma hominis and 6 of 276 clinical isolates of ureaplasma spp. from bordeaux, france (1999 to 2002), were resistant to tetracycline and harbored the tet(m) gene. for m. hominis, we also found an increase in tetracycline resistance and two tet(m)-positive isolates that were susceptible to tetracyclines. | 2008 | 18025113 |
| [immunologic parameters of monkeys infected by urogenital mycoplasmas]. | in monkeys contained in captivity conditions in open-air cages or in group cages human mycoplasmas are often detected: antigens of mycoplasma hominis in blood serum were revealed in 33.3% of cases, and antibodies to it--in 15.6% of cases. igm to m. hominis were detected more often than igg. in 8 monkeys both types of immunoglobulins were detected. rates of detection of ureaplasma urealyticum antigens and specific antibodies were 43.1% and 31.1% respectively, and igg were found more frequently th ... | 2007 | 18038556 |
| trichomonas vaginalis harboring mycoplasma hominis increases cytopathogenicity in vitro. | the parasite trichomonas vaginalis causes one of the most common non-viral sexually transmitted infections in humans. mycoplasmas are frequently found with trichomonads but the consequences of this association are not yet known. in the present study, the effects of t. vaginalis harboring m. hominis on human vaginal epithelial cells and on mdck cells are described. the results were analyzed by light, scanning and transmission electron microscopy, as well as using cell viability assays. there was ... | 2008 | 18040730 |
| genetic variability of the p120' surface protein gene of mycoplasma hominis isolates recovered from tunisian patients with uro-genital and infertility disorders. | among the surface antigens of mycoplasma hominis, the p120' protein was previously shown to elicit a subtle antibody response and appears to be relatively conserved. to get better insight into the evolution of this protein, we analysed the genetic variability of its surface exposed region in 27 m. hominis isolates recovered from the genital tract of tunisian patients with infertility disorders. | 2007 | 18053243 |
| the presence of mycoplasma hominis in isolates of trichomonas vaginalis impacts significantly on dna fingerprinting results. | the genetic characterization of trichomonas vaginalis (protista: trichomonadidae), the causative agent of trichomoniasis in humans, is central to understanding the epidemiology, treatment, drug resistance, and virulence as well as the diagnosis and control of this parasite. various molecular approaches, including dna fingerprinting, have been employed for this purpose, and random amplification of polymorphic dna (rapd) continues to be utilized. however, little attention has been paid to the fact ... | 2008 | 18058131 |
| assessment of chlamydia trachomatis, ureaplasma urealyticum, ureaplasma parvum, mycoplasma hominis, and mycoplasma genitalium in semen and first void urine specimens of asymptomatic male partners of infertile couples. | the purpose of this study was threefold: to compare semen and first void urine (fvu) specimens from asymptomatic infertile men for the detection of chlamydia trachomatis, genital ureaplasma, and genital mycoplasma infections using in-house inhibitor-controlled polymerase chain reaction (pcr)-microtiter plate hybridization assay; to determine the prevalence of those organisms in infertile men in tunisia; and to study the relationship between these bacteria and male infertility. paired urine and s ... | 2008 | 18077823 |
| [study on the pathogens correlated to sexually transmitted diseases in 285 pre-pubertal girls with vulvovaginitis in beijing]. | to study the relationship between vulvovaginitis in pre-pubertal girls and pathogens as chlamydia trachomatis (ct), n. gonorrhoeae (ng), mycoplasma, ureaplasma urealyticum (uu), mycoplasma hominis (mh), m. genitalium (mg), m. fermentans (mf) and m. penetrans (mpe), as well as to find out the proportion of mycoplasma which is correlated to sexually transmitted diseases (std) and aids. methods vulvae swab specimens from 285 pre-pubertal girls with vulvovaginitis (case group) and 128 healthy girls ... | 2007 | 18080562 |
| demographic, clinical and microbiological characteristics of maternity patients: a canadian clinical cohort study. | objective: to determine the demographic, clinical and microbiological characteristics of a representative canadian obstetrical population. design: a one-year cohort study of all maternity patients who were followed to delivery, using detailed patient questionnaires containing more than 60 demographic and clinical variables, and three microbiological evaluations during gestation - first trimester, 26 to 30 weeks, and labour and delivery. outcome measurements included birth weight and gestational ... | 2002 | 18159407 |
| twenty percent of very preterm neonates (23-32 weeks of gestation) are born with bacteremia caused by genital mycoplasmas. | | 2008 | 18166295 |
| maternal infection and risk of preeclampsia: systematic review and metaanalysis. | there are lingering questions regarding the association between maternal infection and preeclampsia. systematic review and metaanalysis was conducted of observational studies that examined the relationship between maternal infection and preeclampsia. forty-nine studies met the inclusion criteria. the risk of preeclampsia was increased in pregnant women with urinary tract infection (pooled odds ratio, 1.57; 95% ci, 1.45-1.70) and periodontal disease (pooled odds ratio, 1.76; 95% ci, 1.43-2.18). t ... | 2008 | 18166297 |
| the alabama preterm birth study: umbilical cord blood ureaplasma urealyticum and mycoplasma hominis cultures in very preterm newborn infants. | this study was undertaken to evaluate the frequency of umbilical cord blood infections with ureaplasma urealyticum and mycoplasma hominis in preterm 23- to 32-week births and to determine their association with various obstetric conditions, markers of placental inflammation, and newborn outcomes. | 2008 | 18166302 |
| detection of a microbial biofilm in intraamniotic infection. | microbial biofilms are communities of sessile microorganisms formed by cells that are attached irreversibly to a substratum or interface or to each other and embedded in a hydrated matrix of extracellular polymeric substances. microbial biofilms have been implicated in >80% of human infections such as periodontitis, urethritis, endocarditis, and device-associated infections. thus far, intraamniotic infection has been attributed to planktonic (free-floating) bacteria. a case is presented in which ... | 2008 | 18166328 |
| perihepatitis and perinephric abscess due to mycoplasma hominis in a kidney transplant patient. | mycoplasma hominis has been incriminated in several genital and extragenital infections. here, we report the first case of perihepatitis associated with a perinephric abscess in a woman who had received a kidney transplant. four months after the transplant, the patient was admitted for perirenal allograft pain, fever, and elevated inflammatory parameters and liver enzyme levels. a renal ultrasonography found a collection of fluid. results of blood and urine analyses were within normal limits. fl ... | 2007 | 18194127 |
| the prevalence of ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis and neisseria gonorrhoeae infections, and the rubella status of patients undergoing an initial infertility evaluation. | to determine the prevalence of positive test for ureaplasma urealyticum (uu), mycoplasma hominis (mh), chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) infections, and their corresponding rubella status when undergoing workup for infertility. | 2008 | 18202910 |
| ureaplasma urealyticum and mycoplasma hominis presence in umbilical cord is associated with pathogenesis of funisitis. | ureaplasma urealyticum (u. urealyticum) and mycoplasma hominis (m. hominis) are known to cause an intrauterine infection for preterm deliveries, but it is not known whether they are actually pathogenically involved in the development of funisitis, chorioamnionitis (cam), and chronic lung disease (cld) in preterm infants. our purpose was to identify u. urealyticum and m. hominis in the umbilical cord, placenta, and tracheal aspirate (ta) or gastric fluid (gf) of preterm infants, and to clarify wh ... | 2007 | 18204300 |
| vaginal il-8 levels are positively associated with candida albicans and inversely with lactobacilli in hiv-infected women. | il-8/cxcl8 is induced during infections, but has not been reported for candida albicans colonization of the female genital tract. cervicovaginal lavage (cvl) samples were collected from 406 hiv-infected women. il-8 levels were evaluated by elisa and compared with levels of c. albicans detected by potassium hydroxide (koh) and pcr. levels of lactobacilli, gardnerella vaginalis and mycoplasma hominis were also determined by pcr. il-8 was significantly higher in samples from women with candida, and ... | 2008 | 18243333 |
| [vaginal colonization by genital mycoplasmas in pregnant and non-pregnant women]. | to compare vaginal colonization by genital micoplasmas in pregnant and non pregnant women and to determine the association between pregnancy and colonization by these microorganisms, samples of exocervix an endocervix from pregnant (n = 80) and non pregnant (n = 65) women, from two health centers of maracaibo, zulia state, venezuela were processed. the mycoplasma-lyo kit (biomérieux laboratories) was used for the culture and identification of genital micoplasmas. in pregnant women, prevalences o ... | 2007 | 18277432 |
| placental histological inflammation and reproductive tract infections in a low risk pregnant population in latvia. | to investigate the correlation of reproductive tract infections (rti) and endogenous vaginal flora at first antenatal consultation with placental histological inflammation. | 2008 | 18307078 |
| reduced susceptibility to tetracyclines is associated in vitro with the presence of 16s rrna mutations in mycoplasma hominis and mycoplasma pneumoniae. | | 2008 | 18356155 |
| [bacteriological finding in the urethra in men with and without non-gonococcal urethritis]. | non-gonococcal urethritis (ngu) is a very common sexually transmitted disease. the etiology of the disease is complex and not completely solved. the aim of this study was to determine the bacteriological finding in the urethra in men with and without non-gonococcal urethritis. | 2007 | 18357907 |
| [the frequency of some infections in patients with coronary artery disease]. | the paper is dedicated to associations between infective agents and coronary artery disease (cad). the study design included examination of a random sample of non-organized population (389 adults aged 25 to 64 years). using immune-enzyme assay, igm and igg antibodies to seven infective and parasite agents (chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, trichomonas vaginalis, candida albicans, opisthorchis felineus, and giardia lamblia), as well as the level of ige were measur ... | 2008 | 18368789 |
| oppa, the ecto-atpase of mycoplasma hominis induces atp release and cell death in hela cells. | in the facultative human pathogen mycoplasma hominis, which belongs to the cell wall-less mollicutes, the surface-localised substrate-binding domain oppa of the oligopeptide permease was characterised as the main ecto-atpase. | 2008 | 18394151 |
| gardnerella vaginalis and lactobacillus sp in liquid-based cervical samples in healthy and disturbed vaginal flora using cultivation-independent methods. | our objective was to determine the morphotype of the adherent bacteria in liquid-based cytology (lbc) in smears with healthy and disturbed vaginal flora. and to use pcr technology on the same fixed cell sample to establish dna patterns of the 16s rna genes of the bacteria in the sample. thirty samples were randomly selected from a large group of cervical cell samples suspended in a commercial coagulant fixative "(boonfix)." pcr was used to amplify dna of five bacterial species: lactobacillus aci ... | 2008 | 18418885 |
| incidence and antimicrobial susceptibilities of genital mycoplasmas in outpatient women with clinical vaginitis in athens, greece. | the incidence and antimicrobial susceptibilities of ureaplasma urealyticum and mycoplasma hominis, isolated from vaginal and endocervical swabs collected from 369 outpatient women, were determined. | 2008 | 18424791 |
| correlations of selected vaginal cytokine levels with pregnancy-related traits in women with bacterial vaginosis and mycoplasmas. | the aim of this study was to examine correlations between vaginal inflammatory cytokines (il-1alpha, il-1beta, il-6 and il-8) and pregnancy-related traits (gestational age, birth-weight, bmi, weight gain during pregnancy and vaginal ph). differences in correlation coefficients were examined among bacterial vaginosis (bv) status and the presence or absence of mycoplasmas. a total of 105 women between the 22nd and 34th week of pregnancy were enrolled in this study. there was a strong negative corr ... | 2008 | 18436310 |
| post-operative mediastinitis, pleuritis and pericarditis due to mycoplasma hominis and ureaplasma urealyticum with a fatal outcome. | post-sternotomy mediastinitis, although infrequent, is a potentially life-threatening complication of cardiac surgery. we report an unusual case of mycoplasma hominis and ureaplasma urealyticum post-surgical mediastinitis with persistent pleural and pericardial effusion. clinical manifestations and response to therapy are described, and the difficulties of establishing the diagnosis are discussed. | 2008 | 18436601 |
| [serum detection of human urogenital mycoplasma]. | the authors compared the detection rate of dna of urogenital mycoplasmas (ureaplasma spp. and m. hominis) in the urogenital tract (ugt) and serum samples by polymerase chain reaction. testing the smears and serum samples from the same patients (n = 112) showed that ureaplasma was more frequently detected in the ugt smears than in the serum samples. there was the same trend towards m. hominis although the difference was not so significant. therefore, the detection of these microorganisms in ugt i ... | 2008 | 18450085 |
| recombinant plasmid constructs expressing gene for antimicrobial peptide melittin for the therapy of mycoplasma and chlamydia infections. | in view of growing number of pathogenic microbial strain resistant to routine antibiotics, antimicrobial peptides become promising agents for the therapy of infectious diseases. we studied in vivo effects of melittin, an antimicrobial peptide expressed in a recombinant plasmid vector, on infection with urogenital pathogens chlamydia trachomatis, mycoplasma hominis, and mycoplasma gallisepticum. we obtained recombinant plasmid constructs, where melittin gene is under the control of tetracycline-d ... | 2007 | 18457054 |
| presence of chlamydia, mycoplasma, ureaplasma, and other bacteria in the upper and lower genital tracts of fertile and infertile populations. | the genital mycoplasmas (mycoplasma hominis and ureaplasma urealyticum) and chlamydia trachomatis have been implicated as possible etiologic factors in infertility. their role in patients with infertility needs to be further defined. | 1993 | 18475323 |
| amniotic fluid glucose concentration: a marker for infection in preterm labor and preterm premature rupture of membranes. | amniotic fluid gram stain and culture have been utilized as laboratory tests of microbial invasion of the amniotic cavity. the gram stain of amniotic fluid has a low sensitivity in the detection of clinical infection or microbial invasion of the amniotic cavity, and amniotic fluid culture results are not immediately available for management decisions. glucose concentration is used to diagnose infection in other sites such as cerebrospinal fluid. | 1994 | 18475339 |
| genital mycoplasmas in placental infections. | the involvement of the genital mycoplasmas ureaplasma urealyticum and mycoplasma hominis in complications of pregnancy has remained controversial especially because these microorganisms are frequent colonizers of the lower genital tract. recovery of bacteria from the placenta appears to be the sole technique to represent a true infection and not vaginal contamination. therefore, we investigated the presence of genital mycoplasmas, aerobic and anaerobic bacteria, and fungi in human placentas and ... | 1994 | 18475351 |
| perihepatic adhesions: another look. | the objective of our study was to determine if pelvic inflammatory disease (pid) was the only cause of perihepatic adhesions. | 1995 | 18475406 |
| chronic endometritis and positive mycoplasma cultures: is there a correlation? | this study was undertaken to assess the impact of mycoplasma strains (mycoplasma hominis or ureaplasma urealyticum) on the development of chronic endometritis. | 1995 | 18475413 |
| in vitro susceptibility of ureaplasma urealyticum and mycoplasma hominis isolates in argentina. | our goal was to determine the in vitro susceptibility of ureaplasma urealyticum and mycoplasma hominis isolates to several antibiotics in argentina. | 1995 | 18476048 |
| significance of genital mycoplasmas in pelvic inflammatory disease: innocent bystander! | our objective was to determine the role of mycoplasma hominis and ureaplasma urealyticum in pelvic inflammatory disease (pid). | 1996 | 18476105 |
| role of genital mycoplasmas in bacteremia: should we be routinely culturing for these organisms? | objective: the purpose of this study was to examine the role of the genital mycoplasmas mycoplasma hominis and ureaplasma urealyticum as causes of bacteremia in a tertiary referral obstetrical, gynecological, and neonatal intensive care facility, over a period of 12 years from 1983 to 1994 inclusively.methods: all clinically significant blood cultures were reviewed and the percentage of septicemic episodes for genital mycoplasmas was compared to the total isolation rate, including conventional b ... | 1996 | 18476120 |
| molecular quantification of gardnerella vaginalis and atopobium vaginae loads to predict bacterial vaginosis. | bacterial vaginosis (bv) is a poorly detected public health problem that is associated with preterm delivery and for which no reliable diagnostic tool exists. | 2008 | 18513147 |
| prevalence of mycoplasma genitalium, mycoplasma hominis and chlamydia trachomatis among danish patients requesting abortion. | the aim of the study was to determine lower genital tract carriage rate of mycoplasma genitalium (m. genitalium) and to compare it to the carriage rates of mycoplasma hominis (m. hominis ) and chlamydia trachomatis (c. trachomatis) among 102 women requesting termination of pregnancy at the horsens hospital in denmark. real-time pcr was used for the detection of bacterial dna, and the presence of antibodies to the three microorganisms was determined by elisa and immunoblotting. real-time pcr dete ... | 2008 | 18570048 |
| quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems. | transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ... | 2008 | 18579722 |
| detection of chlamydia trachomatis and mycoplasma hominis, genitalium and ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria. | chlamydia trachomatis and mycoplasma hominis, mycoplasma genitalium, and ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological diagnosis. to determine the occurrence of chlamydia trachomatis, mycoplasma hominis, mycoplasma genitalium, and ureaplasma urealyticum in patients with sterile pyuria. | 2008 | 18614434 |
| [preterm premature rupture of membranes and ureaplasma urealyticum]. | the purpose of this study was to evaluate the prevalence of the maternal lower genital tract colonization by ureaplasma urealiticum and mycoplasma hominis in patiens with preterm premature rupture of the membranes. | 2008 | 18646667 |
| mycoplasma hominis wound infection after a vascular allograft. | cases of mycoplasma hominis infections after allograft are rare. we report a case of m. hominis wound infection after a vascular allograft. the allograft was positive before having any contact with the recipient, and our investigation suggests that m. hominis may have been transmitted from the donor to the recipient. it is not clear, however, whether specific diagnosis of m. hominis should be performed on tissue before grafting in order to prevent such donor-to-host transmission. | 2008 | 18649944 |
| comparative in vitro activities of the investigational fluoroquinolone dc-159a and other antimicrobial agents against human mycoplasmas and ureaplasmas. | the in vitro susceptibilities of 151 unique clinical isolates of mycoplasma pneumoniae, mycoplasma hominis, mycoplasma fermentans, mycoplasma genitalium, and ureaplasma species to dc-159a, an investigational fluoroquinolone, in comparison with those to other agents were determined. macrolides were the most active agents against m. pneumoniae and m. genitalium, whereas clindamycin was most active against m. hominis. dc-159a mics were <or=0.5 microg/ml for all mycoplasma species and <or=4 microg/m ... | 2008 | 18663020 |
| successful treatment of post-neurosurgical intracranial mycoplasma hominis infection using gatifloxacin. | we describe two cases of intracerebral infection with mycoplasma hominis following neurosurgery, the first after removal of a colloid cyst, the other after a craniotomy following a motor vehicle accident (mva). both infections were successfully treated with parenteral gatifloxacin, with ongoing clindamycin or moxifloxacin for associated osteomyelitis. | 2008 | 18708262 |
| screening for bacterial pathogens in semen samples from infertile men with and without leukocytospermia. | we aimed to study the correlation between leukocyte counts in semen and bacterial pathogens in seminal samples of infertile men, and to establish the minimum leukocyte count associated with significant bacteriospermia. a total of 116 patients who underwent evaluation of fertility were investigated using routine semen analysis according to the guidelines of the who and bacterial pathogens analysis by culture and in-house pcr assay. the overall prevalence of bacteriospermia in semen samples was 56 ... | 2008 | 18727730 |
| activity of moxifloxacin against the urogenital mycoplasmas ureaplasma spp., mycoplasma hominis and mycoplasma genitalium and chlamydia trachomatis. | the activity of moxifloxacin was compared with that of other antimicrobial agents against 54 strains of ureaplasma spp., 54 strains of mycoplasma hominis, 14 strains of mycoplasma genitalium, and 44 strains of chlamydia trachomatis. moxifloxacin inhibited 90% of all isolates at a concentration </=1 mg/l, being the most active compound against c. trachomatis and sharing the highest activity with garenoxacin and gemifloxacin against mycoplasmas. moxifloxacin killed the 30 mycoplasma isolates teste ... | 2008 | 18727805 |
| successful treatment of multiple subdural empyemata caused by mycoplasma hominis in a newborn. | we report a case of neonatal subdural empyema caused by mycoplasma hominis. the infant sustained severe birth-related eye injury and subsequently developed multiple subdural empyemata. this report illustrates the clinical presentation of this unusual infection and the crucial role of neurosurgical intervention and specific antimicrobial therapy for its successful management. | 2009 | 18776733 |
| mycoplasma hominis meningitis in a neonate: case report and review. | despite frequent colonization with mycoplasma hominis, the invasive disease is rare in neonates. this study describes a neonatal case with meningitis in which m. hominis was isolated from a cerebrospinal fluid sample by culture and detected by pcr. the m. hominis infection was confirmed by elevated metabolic inhibition titers against the isolated m. hominis strain and anti-m. hominis antibodies in serum samples. minocycline and moxifloxacin were effective against m. hominis, which caused meningi ... | 2008 | 18790539 |
| [leukemoid reaction in extremely immature preterm infants]. | extremely immature preterm infants rarely present with a leukocytosis exceeding 30,000/microl. the pathogenetic sequence leading to leukemoid reactions in non-malignant diseases remains to be elucidated. potential triggers for leukemoid reactions in premature infants include prenatal corticosteroids, chorioamnionitis and funisitis or systemic infection. in the two-year period from 2006 to 2007 all infants with a gestational age of less than 26 weeks were screened for leukocytosis. among our case ... | 2008 | 18956273 |
| multiplex pcr for the detection of urogenital pathogens in mothers and newborns. | two duplex pcr assays were established for the detection of c. trachomatis (ct), n. gonorrhoeae (gc), m. hominis (mh), and u. urealyticum (uu). these assays were used on clinical specimens obtained from women with premature rupture of membrane or post partum fever, from preterm infants, as well as from women with uneventful pregnancies and their babies delivered vaginally at term. the analytical sensitivity of the duplex pcr assays with internal controls incorporated is 7.0, 19.0, 5x10(3) and 7x ... | 2007 | 19105324 |
| congenital and opportunistic infections: ureaplasma species and mycoplasma hominis. | there is strong evidence from clinical and experimental animal studies that ureaplasmas can invade the amnionic sac and induce an inflammatory response resulting in chorioamnionitis, preterm labor and neonatal lung injury. the ability of ureaplasma spp. and mycoplasma hominis to cause pneumonia, bacteremia, and meningitis in newborns can no longer be questioned. the association of ureaplasma spp. with bronchopulmonary dysplasia has been supported by the majority of observational studies, but pro ... | 2009 | 19109084 |
| ureaplasma parvum or mycoplasma hominis as sole pathogens cause chorioamnionitis, preterm delivery, and fetal pneumonia in rhesus macaques. | the authors assess causal, cellular and inflammatory links between intraamniotic infection with ureaplasma parvum or mycoplasma hominis and preterm labor in a nonhuman primate model. long-term catheterized rhesus monkeys received intraamniotic inoculations of clinical isolates of ureaplasma parvum serovar 1, m hominis, media control or physiological saline. genital mycoplasmas were quantified in amniotic fluid (af) and documented in fetal tissues by culture and pcr. in association with elevated ... | 2009 | 19122105 |
| [duration of preservation of viable cells, dna, and antigens of mycoplasma hominis and ureaplasmas in human serum at 37 degrees c]. | how long the viable cells of m. hominis, ureaplasma spp., u. urealyticum, u. parvum, and their antigens retained in human serum at 37 degrees c was investigated. m. hominis cells were shown to hold their viability within 12 days with a gradual titer drop, the antigens being also detected within 12 days whereas intracellular and extracellular dnas were seen within 40 days (an observation time). under the same conditions, ureaplasma cells died after 24 hours, their antigens were disrupted followin ... | 2008 | 19140410 |
| [results of assessing the quality of chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum dna by polymerase chain reaction in 2006-2007)]. | analyzing the results of detection of c. trachomatis, m. hominis, and u. urealyticum dna by the participants of the federal external quality assessment system in 2006-2007 indicated that the share of the fluorescent-probe amplification technique both in the real-time mode and by the end-point tended to increase. the above tendency coincided with the other--the higher proportion of laboratories detecting m. hominis and u. urealyticum at low concentrations (as low as 10(3) copies/ml) although no d ... | 2008 | 19140411 |
| human papillomaviruses and genital co-infections in gynaecological outpatients. | high grade hpv infections and persistence are the strongest risk factors for cervical cancer. nevertheless other genital microorganisms may be involved in the progression of hpv associated lesions. | 2009 | 19216747 |
| azithromycin treatment for nongonococcal urethritis negative for chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum, and ureaplasma urealyticum. | some patients with nongonococcal urethritis (ngu) are negative for chlamydia trachomatis, mycoplasmas, and ureaplasmas. the optimal antimicrobial chemotherapy for such ngu has not fully been clarified. we assessed the efficacy of azithromycin for treatment of nonmycoplasmal, nonureaplasmal, nonchlamydial ngu (nmnuncngu). thirty-eight men whose first-pass urine was negative for chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum, and ureaplasma urealyticum were tre ... | 2009 | 19228227 |
| the pediatric infectious disease journal(r) newsletter: march 2009. | | 2009 | 19252427 |
| comparative in vitro susceptibilities of human mycoplasmas and ureaplasmas to a new investigational ketolide, cem-101. | mics were determined for an investigational ketolide, cem-101, and azithromycin, telithromycin, doxycycline, levofloxacin, clindamycin, and linezolid against 36 mycoplasma pneumoniae, 5 mycoplasma genitalium, 13 mycoplasma hominis, 15 mycoplasma fermentans, and 20 ureaplasma isolates. all isolates, including two macrolide-resistant m. pneumoniae isolates, were inhibited by cem-101 at < or = 0.5 microg/ml, making cem-101 the most potent compound tested. | 2009 | 19258276 |
| multiplex pcr testing detection of higher-than-expected rates of cervical mycoplasma, ureaplasma, and trichomonas and viral agent infections in sexually active australian women. | knowing the prevalence of potential etiologic agents of nongonococcal and nonchlamydial cervicitis is important for improving the efficacy of empirical treatments for this commonly encountered condition. we describe four multiplex pcrs (mpcrs), designated vdl05, vdl06, vdl07, and vdl09, which facilitate the detection of a wide range of agents either known to be or putatively associated with cervicitis, including cytomegalovirus (cmv), enterovirus (ev), epstein-barr virus (ebv), varicella-zoster ... | 2009 | 19261782 |
| the significance of chlamydia trachomatis in urethritis and prostatitis - differences in therapeutic approach - croatian experience. | we examined a total of 1014 patients over 18 years of age; 252 with urethritis and 762 with chronic prostatitis syndrome. the mean age of patients with urethritis was 32.7 and with prostatitis syndrome 37.6 years. clinical symptoms of urethritis were present from a few days to several months. in patients with chronic prostatitis syndrome, symptoms were present for at least 3 months. chlamydia trachomatis alone was confirmed in 26 (10%) and in combination with ureaplasma urealyticum in 6 (2%) pat ... | 2009 | 19297275 |
| prevalence of urogenital mycoplasmas and ureaplasmas in women after kidney transplantation. | the prevalence of urogenital mycoplasmas and ureaplasmas in kidney transplant and hemodialyzed patients was studied. | 2009 | 19300187 |
| correlation of mycoplasma with unexplained infertility. | the role of genital mycoplasmas, particularly ureaplasma urealyticum, in the etiology of infertility has been very controversial. an attempt has been made to explore the role of mycoplasma in unexplained infertility. | 2009 | 19322575 |
| a high nugent score but not a positive culture for genital mycoplasmas is a risk factor for spontaneous preterm birth. | this study was performed to evaluate the relationship among the nugent score for the diagnosis of bacterial vaginosis (bv), the results of vaginal fluid culture for genital mycoplasmas, and the subsequent occurrence of preterm birth. | 2009 | 19330704 |
| the expansion of the microbiological spectrum of brain abscesses with use of multiple 16s ribosomal dna sequencing. | background. brain abscess is commonly treated using empirically prescribed antibiotics. thus, a comprehensive study of bacterial organisms associated with brain abscess is essential to define the best empirical treatment for this life-threatening condition. methods. we prospectively compared cultures to single and multiple sequenced 16s ribosomal dna polymerase chain reaction amplifications (by cloning and/or pyrosequencing) of cerebral abscesses in 20 patients from 2 hospitals in marseilles, fr ... | 2009 | 19335164 |
| simultaneous identification of 14 genital microorganisms in urine by use of a multiplex pcr-based reverse line blot assay. | the aim of this study was to develop and evaluate a sensitive method for the simultaneous identification of 14 urogenital potential pathogens. a multiplex pcr-based reverse line blot (mpcr/rlb) assay was developed to detect 14 urogenital pathogens or putative pathogens, namely trichomonas vaginalis, streptococcus pneumoniae, neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma parvum, u. urealyticum, gardnerella vaginalis, haemophilus influenzae, herpes simplex virus type 1 (hsv1) and hsv2, ... | 2009 | 19357202 |
| prevalence of ureaplasma urealyticum and mycoplasma hominis in women with chronic urinary symptoms. | to assess the prevalence of ureaplasma urealyticum and mycoplasma hominis in women experiencing chronic urinary symptoms. | 2009 | 19371925 |
| [two cases of septic arthritis by mycoplasma hominis after total knee replacement arthroplasty]. | mycoplasma hominis has been related with pelvic inflammatory illnesses and postpartum and neonatal infections. extragenital m. hominis infections are rare, but septicemia, septic arthritis, wound infection, meningitis, and other infections in immunocompromised patients have also been described. here we report two cases of septic arthritis caused by m. hominis in patients following total knee replacement arthroplasty. after the surgery, the patients presented with knee pain and clinical signs of ... | 2009 | 19411780 |