| biotransformation and distribution in blood of cyclosporine and its metabolites. | | 1986 | 3787711 |
| isolation of coxiella burnetii from human sources. | a total of 153 human milk samples were subjected to capillary agglutination test (cat) for determination of c. burnetii antibodies using phase-i cat antigen and 22 (14.37%) samples were found to be positive. twenty two cat positive milk samples were further processed for the isolation of c. burnetii. of seven samples positive in first screening, only four revealed presence of c. burnetii by giving specific seroconversion in guineapigs. similarly, 16 aborted placentas were processed for c. burnet ... | 1986 | 3793389 |
| overlapping deletion in two spontaneous phase variants of coxiella burnetii. | chromosomal dna from the nine mile phase i strain of coxiella burnetii (cb9mic7) was cloned into the cosmid vector phc79. the resulting gene library was probed with a radiolabelled haeiii fragment present in the parental strain but absent from a spontaneously derived nine mile phase ii strain (cb9miic4). the insert, which includes the missing haeiii fragment, was 38.5 kb in length. when dna from this cosmid clone was hybridized to genomic dna of the parental cb9mic7 and its derivative cb9miic4, ... | 1986 | 3794655 |
| [effect of a helminth invasion on the human staphylococcal carrier state]. | | 1986 | 3796454 |
| [q fever]. | | 1987 | 3803237 |
| perturbation of differentiated functions in vivo during persistent viral infection. iii. decreased growth hormone mrna. | lymphocytic choriomeningitis virus (lcmv) persistent infection that results from the inoculation of c3h/st newborn mice causes growth hormone (gh) deficiency and associated disease characterized by both reduced weight and serum glucose levels. molecular analysis of pituitary nucleic acids shows gh deficient mice have, on average, fivefold reduced levels of gh mrna although the histopathology of such gh producing cells is normal. northern blots indicate that the length of gh mrna is comparable in ... | 1987 | 3811236 |
| sheep-associated outbreak of q fever, idaho. | between feb 1 and aug 31, 1984, an outbreak of 18 symptomatic cases of q fever occurred in idaho; these numbers represent an increase over the three cases reported in 1982 and the five reported in 1983. four of the patients in the outbreak required hospitalization for two to five weeks; there were no fatalities. eight of the cases had documented q fever hepatitis, and one had pneumonia. all 18 of the 1984 cases for whom information was available were epidemiologically linked to visiting or worki ... | 1987 | 3813754 |
| rem-sleep deprivation-induced increase in ethanol intake: role of brain monoaminergic neurons. | the ethanol intake of long-evans male rats was recorded before, during and after deprivation of rapid eye movement (rem) sleep produced with the flowerpot technique modified by using a cuff pedestal and electrified grid floor instead of water. ethanol intake increased significantly during rem-sleep deprivation. a rebound decrease in ethanol drinking was then observed during the rem-rebound phase immediately after the termination of rem-sleep deprivation. because rem-sleep deprivation has been re ... | 1986 | 3814349 |
| effects of pentachlorophenol on the in vitro and in vivo antibody response. | the effects of pentachlorophenol (pcp) on selected parameters reflecting immunocompetence of female b6c3f1 mice were measured following subchronic exposure (14 d) and direct exposure in culture. daily exposure was by gastric intubation of 10, 30, or 100 mg/kg of technical-grade pcp (pcp-t), or 100 mg/kg of ec-7, a pcp preparation purified to reduce contamination (pcp-p), or 100 mg/kg of the vehicle, corn oil. there were no effects on the antibody responses of spleen-cell suspensions from either ... | 1987 | 3820337 |
| neutrophil kinetics in o2-exposed rabbits. | hyperoxic injury results in an influx of polymorphonuclear leukocytes (pmn) into the lung. to better understand the role of the pmn in this injury, kinetic studies were used to assess the survival of pmns in the circulation. the rate of deposition of pmns in the lungs of rabbits exposed to hyperoxia was also examined. the half-lives (t1/2) of [3h]thymidine-labeled pmns in the circulation in rabbits exposed to air or to 95% o2 for less than or equal to 48 h varied between 3.9 and 4.5 h. after 72 ... | 1986 | 3827992 |
| diagnosis of chlamydial infection in the female urogenital tract, and treatment with doxycycline. | microbiologic diagnostic tests (comprising phase-contrast microscopy, fungal cultures, staining cytology, and immunofluorescence microscopy with monoclonal antibodies against chlamydia trachomatis) were performed in 100 women with recurrent urogenital infections. the incidence and localization of chlamydia trachomatis are reported. the therapeutic efficacy of systemic treatment with doxycycline in this group was evaluated. all of the 25 patients in whom treatment and treatment results could be s ... | 1986 | 3829087 |
| influence of portocaval anastomosis (pca) on tissue po2 of renal cortex and skeletal muscle in rats with liver cirrhosis. | | 1985 | 3832875 |
| studies on the pathogenicity of nocardia species inoculated into mice intraperitoneally. | | 1985 | 3833726 |
| [changes in the degradation time for acetylcholine receptors in relation to age]. | a 125i-bungarotoxin labelling study of the acetylcholine receptor on the nerve-muscle junctions in the course of aging in the rat is reported. attention is drawn to the fact that aging leads to an appreciable slowing down of the receptor degradation time, whereas no change occurs in its location. | 1985 | 3837673 |
| liposome mediated dna-transfer into mammalian cells. | we have investigated the interaction of mammalian cells with liposome encapsulated dna. tissue cultured mammalian cells were exposed to large, unilamellar phosphatidyl serine liposomes containing dna molecules from different animal cells or prokaryotic organisms. the liposomes bind rapidly to the surface and are taken up by the cells and significant proportion of the encapsulated dna is transported to the nuclei. transient expression of the foreign genetic material could be detected in high perc ... | 1985 | 3837979 |
| [results of a pilot study of hyaluronidase as an adjunct to cytostatic therapy in malignant diseases]. | 46 patients (17 myelomas, 11 malignant lymphomas, 8 mammary carcinomas, 7 head and neck carcinomas, 2 gastrointestinal carcinomas and 1 ovarian carcinoma) were treated with permease prepared of bovine testes by sanabo. 7500 i.u. were given either intramuscular one hour before cytostatic chemotherapy or intraperitoneally with cytostatic agents. there were 2 cases of local irritation on the site of injection and 1 case of reversible anaphylactoid reaction. results achieved in patients treated with ... | 1985 | 3838606 |
| molsidomine on cardiovascular leukotriene d4 actions. | anaesthetized open-chest dogs were used to study the effects of intracoronary leukotriene d4 (ltd4; 0.5 micrograms/kg) on haemodynamics, the electrocardiogram (ecg), coronary blood flow in the left circumflex artery and coronary resistance in the absence or presence of the antianginal drug molsidomine (500 micrograms/kg i.v.). ltd4 increased left ventricular (lv) filling pressure from 6.5 +/- 3.8 to 14.7 +/- 3.2 mm hg (p less than 0.01), decreased lv dp/dtmax from 2,500 +/- 200 to 1,240 +/- 205 ... | 1985 | 3839292 |
| myosin light chain phosphorylation and isometric twitch potentiation in intact human muscle. | the effects of a single voluntary contraction of the quadriceps muscle group on phosphate incorporation into the phosphorylatable light chains (p-light chains) of fast and slow myosin isolated from the vastus lateralis muscle and potentiation of the electrically stimulated twitch tension was studied in intact human muscle. twitch potentiation was maximal 20 s after the voluntary contraction. thereafter, twitch potentiation declined, but was still significantly higher than pre-contraction values ... | 1985 | 3839303 |
| [study of reproductive pathology in cattle in central africa: survey of chlamydiosis and q fever]. | | 1985 | 3842175 |
| a fatal case of q fever hepatitis in a child. | a two-year-old boy of arabic extraction presented with progressive jaundice and prolonged pyrexia. both igm and igg immunofluorescent antibody titers for q fever were 1:1280. two goats and one cow of the domestic animals owned by the family also had positive antibody titers against q fever. in spite of antibiotic treatment with tetracyclines and chloramphenicol, the hepatic involvement progressed gradually. on the twentieth day of admission the child succumbed from hepatic failure. this child pr ... | 1985 | 3843239 |
| q fever and experimental sheep. from the international council for laboratory animal science. | | 1985 | 3845065 |
| [the last yanomami: tobacco as an energy source and as a cariostatic]. | | 1985 | 3858660 |
| enrichment of human milk lymphocytes by discontinuous density gradient sedimentation. | a simple method is described for the enrichment of lymphocytes from human colostrum and early milk. washed leucocytes from colostrum or milk were suspended in balanced salt solution containing 25% human serum and separated by discontinuous density gradient centrifugation over lymphoprep (1.077 g/ml) and nycodenz (1.070 g/ml). the cell fraction harvested from the interface between these two media contained a mean of 56% lymphocytes (9-92%), as compared to 5.6% (0-17%) in the unseparated samples, ... | 1985 | 3873781 |
| plasmodium falciparum malaria: band 3 as a possible receptor during invasion of human erythrocytes. | human erythrocyte band 3, a major membrane-spanning protein, was purified and incorporated into liposomes. these liposomes, at nanomolar concentrations of protein, inhibited invasion of human erythrocytes in vitro by the malaria parasite plasmodium falciparum. liposomes containing human band 3 were ten times more effective in inhibiting invasion than those with pig band 3 and six times more effective than liposomes containing human erythrocyte glycophorin. liposomes alone or liposomes containing ... | 1985 | 3880920 |
| isolation, experimental transmission, and characterization of causative agent of potomac horse fever. | potomac horse fever, a disease characterized by fever, anorexia, leukopenia, and occasional diarrhea, is fatal in approximately 30 percent of affected animals. the seasonal occurrence of the disease (june to october) and evidence of antibodies to the rickettsia ehrlichia sennetsu in the serum of convalescing horses suggested that a related rickettsia might be the causative agent. such an agent was isolated in cultured blood monocytes from an experimentally infected pony. this intracytoplasmic or ... | 1985 | 3880925 |
| vesicles and cisternae in the trans golgi apparatus of human fibroblasts are acidic compartments. | recently we demonstrated that low-ph compartments can be visualized with the electron microscope using a basic congener of dinitrophenol, 3-(2,4-dinitroanilino)-3'-amino-n-methyldipropylamine (damp), which concentrates in acidic compartments and can be detected by immunocytochemistry with a monoclonal anti-dinitrophenol antibody. we now report that damp also accumulates in cisternae and vesicles associated with the trans face of the golgi apparatus. damp rapidly leaves this compartment when cell ... | 1985 | 3882239 |
| fatal fungemia with arthritic involvement caused by trichosporon beigelii in a bone marrow transplant recipient. | | 1985 | 3882853 |
| lipid peroxidation-induced inhibition of gamma-aminobutyric acid uptake in rat brain synaptosomes: protection by glucocorticoids. | incubation of rat brain synaptosomes with xanthine and xanthine oxidase (x/xo) resulted in an inhibition of gamma-aminobutyric acid (gaba) uptake. the inhibitory effects of x/xo were temperature- and time-dependent, and were characterized by an increased km for gaba and a decreased vmax. inhibition of gaba uptake by x/xo was associated with both the formation of malonyldialdehyde (mda) and conjugated dienes, indicating that lipid peroxidation was involved. studies with catalase, superoxide dismu ... | 1985 | 3882888 |
| inhibition of glucagon release of isolated islets of langerhans by monoclonal antibodies. | the presence of islet cell cytoplasmic antibodies (ica) and islet cell surface antibodies (icsa) at the time of diagnosis of type 1 (insulin-dependent) diabetes mellitus has been taken as evidence that autoimmune mechanisms are involved in the pathogenesis of the disease. the demonstration that icsa in the presence of complement are preferentially lytic for beta-cells may be important in defining the role of these autoantibodies in the pathogenesis of type 1 diabetes. because of the polyclonalit ... | 1985 | 3886411 |
| a mutagen precursor in chinese cabbage, indole-3-acetonitrile, which becomes mutagenic on nitrite treatment. | after treatment with nitrite, chinese cabbage showed direct-acting mutagenicity on salmonella typhimurium ta100 inducing 3100 revertants per g. one of the mutagen precursors that became mutagenic after nitrite treatment was isolated, and identified as indole-3-acetonitrile. after treatment with nitrite, 1 mg of indole-3-acetonitrile induced 17 400 revertants of ta100 and 21 000 revertants of ta98 without s9 mix. | 1985 | 3889600 |
| seroepidemiology of q fever among domestic animals in nova scotia. | we used the indirect microimmunofluorescence test to determine the presence of antibodies in sheep, cattle, goat, cat, and dog sera to phase i and ii coxiella burnetii antigens in nova scotia. only 6.7 per cent of the 329 sheep tested had antibodies to phase ii antigen and none had antibodies to phase i. of 29 goats tested, 7 per cent and 3.5 per cent had antibodies to phase ii and phase i antigens, respectively. in contrast, 23.8 per cent of the 214 cattle tested had antibodies to phase ii anti ... | 1985 | 3890569 |
| a possible role for natural killer cells in providing protection against plasmodium berghei in early stages of infection. | beige mutant mice, which are deficient in natural killer (nk) cells, exhibited a significantly higher parasitaemia than the parental c57bl/6 strain between days 4 and 10 after infection with plasmodium berghei. within 8-12 days after infection 70% of beige mice were dead but no deaths occurred in the parental strain until day 16. the median survival time of the beige mice (10 days) was significantly lower than that of the parental strain (22 days). it appears that nk cells may be protective in t ... | 1985 | 3891603 |
| [clinical aspects observed during an epidemic of 415 cases of q fever]. | the clinical findings during a major epidemic of q-fever which affected 415 people in the val de bagnes (valais, switzerland) in the autumn of 1983 are reported. q-fever symptoms were evident in 191 cases but inconspicuous or absent in 224 cases. the symptoms most frequently reported were prolonged high fever, headaches, severe exhaustion, loss of appetite, cough and myalgia. amongst disorders which accompany acute q-fever, pneumonia and granulomatous hepatitis are very frequent, while myoperica ... | 1985 | 3892664 |
| current diagnosis of infective endocarditis. | an analysis was made of 91 cases of infective endocarditis (ie) with regard to causative organisms and their sensitivities to various antibiotics, the clinical features of the disease, the laboratory test results and other items were important in establishing a diagnosis of ie. the number of cases of ie has shown a tendency to increase in recent years, particularly in the number of elderly patients, and the ratio of total cases consisting of prosthetic valve endocarditis (pve) has shown a sharp ... | 1985 | 3894710 |
| q fever in infancy: a review of 18 cases. | infection with coxiella burnetti (q fever) was diagnosed in 18 children younger than 3 years of age in the netherlands during a 16-month period. the diagnosis was confirmed serologically by means of a complement-fixation test and immunofluorescence for igm determination. a summary of the clinical, hematologic, serologic and epidemiologic features is given. four children had relapsing episodes of fever during several months. the problem of childhood infection with c. burnetii, particularly in rel ... | 1985 | 3895176 |
| thioredoxin. | | 1985 | 3896121 |
| thioredoxin. | | 1985 | 3896121 |
| immunofluorescence serology. a tool for prognosis of q-fever. | the indirect immunofluorescence antibody test is currently the method of choice for q-fever laboratory diagnosis. it permits the detection of igg-, igm-, and iga-specific antibodies against the two phases of coxiella burnetii. sera from 20 cases of c. burnetii infection have been examined. only total igg against phase ii were detected in cryptic infections. in acute q-fever cases, the appearance of total igg antibodies against phase i was a sign of aggravation, while igm titers remained low. in ... | 1985 | 3896627 |
| evaluation of the complement fixation and indirect immunofluorescence tests in the early diagnosis of primary q fever. | a comparison was made of the performance of the commonly used complement fixation test and the more recently developed indirect immunofluorescence test in the early diagnosis of q fever. the 303 sera tested were from 181 patients who contracted q fever during an outbreak in switzerland in 1983. specific igm antibodies were detected by the immunofluorescence test in 53% and 89% of sera obtained during the first and second week respectively after onset of illness. with the complement fixation test ... | 1985 | 3899636 |
| selective detection of 3-deoxymannooctulosonic acid in intact lipopolysaccharides by spin-echo 13c nmr. | the 3-deoxy-d-mannooctulosonic acid (kdo) region of lipopolysaccharides (lps) from the heptoseless mutant salmonella minnesota r595 and inner core and heptoseless mutants derived from escherichia coli k12 was studied by 13c nmr spectroscopy. a spin-echo spectral editing technique was employed for the selective detection of the quaternary anomeric carbon of ketosidically linked kdo. only two quaternary carbon resonances attributable to kdo were detected in the anomeric carbon spectral region of e ... | 1985 | 3902810 |
| q-fever. the liver and bone marrow pathology. | eighteen liver and seven bone marrow biopsies from 44 patients with clinically and serologically proven q-fever seen during a recent outbreak were studied. highly distinctive fibrin-ring granulomas were found in seven liver and four bone marrow specimens. lipid or nonspecific granulomas often containing neutrophils and variable numbers of giant cells were noted in 13 livers and seven bone marrows. a wide variety of nongranulomatous histological changes, frequently including steatosis and nonspec ... | 1985 | 3904493 |
| immunoglobulin responses in acute q fever. | knowledge of the development of different classes of antibody during the course of acute q fever is important to the clinician for interpreting a patient's serological test results. in the present study, the appearance of antibodies to coxiella burnetii phases i and ii was determined for a period of 1 year. a total of 683 sera from 191 patients with symptomatic q fever were evaluated by the complement fixation and indirect immunofluorescence (immunoglobulins m and g [igm, igg]) tests. these pati ... | 1985 | 3908466 |
| [juvenile periodontitis. i: etiopathogenetic aspects]. | | 1985 | 3911054 |
| [sonography of a mesenteric lymphangioma]. | the ultrasonic findings of a large mesenteric lymphangioma in a man of 62 years of age are reported. haemorrhage in some of the cystic spaces of the lymphangioma had caused an acute abdomen. | 1985 | 3911408 |
| treatment of plasma cell neoplasm with recombinant leukocyte a interferon and human lymphoblastoid interferon. | thirty cases of plasma cell neoplasms (24 multiple myeloma, one plasma cell leukemia, and three primary macroglobulinemia) were treated with two kinds of highly purified alpha-interferons, recombinant human leukocyte interferon (rifn-alpha a) (16 cases) and human lymphoblastoid interferon (hlbi) (14 cases). partial remission (pr) was obtained in two of 16 evaluable cases treated with rifn-alpha a and in two of 12 evaluable cases treated with hlbi. if minor response (mr) was included, responses w ... | 1985 | 3917376 |
| treatment of septic arthritis due to mycobacterium kansasii. | | 1985 | 3919820 |
| [differential affinity of pathogenic species of microorganisms for a set of lectins detectable by the sandwich method using fluorescein isothiocyanate (fitc)]. | the qualitative differences in the affinity of concanavalin a (con a), wheat-germ agglutinin (wga) and phaseolus vulgaris lectin to the surface of 10 microbial strains inducing various diseases in humans and agricultural animals have been demonstrated by means of the indirect immunofluorescence tests. enterobacteria, coxiella burnetii and bacillus anthracis have been found to possess pronounced affinity to con a and wga, while rickettsia prowazekii, francisella tularensis and brucella abortus, a ... | 1985 | 3920844 |
| [preliminary studies on the occurrence of rickettsiae of the spotted fever group in rhipicephalus sanguineus captured in suburban areas]. | in the urban periphery of rome, two sub-zones could be distinguished, characterized by uncultivated steppeland and by small shacks with large quantities of domestic refuse, respectively. rhipicephalus sanguineus appeared to be typical of this second sub-zone. r. sanguineus adults and nymphae were captured in different seasonal periods and were checked for the prevalence of rickettsiae of the spotted fever (sf) group. moreover, larvae from hatched eggs, laid by captured r. sanguineus females, wer ... | 1985 | 3924079 |
| the urinary excretion of bacterial amino-acid metabolites by rats fed saccharin in the diet. | administration of a diet containing 7.5% saccharin to adult male rats for 40 days caused a three- to four-fold increase in the daily excretion of indican and rho-cresol. indican is formed from indole which is a microbial metabolite of tryptophan, whilst rho-cresol is formed by the gut flora from tyrosine. the excretion of phenol, which is also a microbial metabolite of tyrosine, was abolished by saccharin administration for 40 days. analysis of urines collected at 13, 18 and 24 months during a t ... | 1985 | 3924804 |
| pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells. | pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ... | 1985 | 3925713 |
| the effects of aerosols of carbamylcholine, serotonin and propranolol on the ventilatory response to co2 in guinea pigs and comparison with the effects of histamine and sulfuric acid. | a new method is introduced to recognize the acute pulmonary effect of airborne contaminants in guinea pigs. the method avoids anaesthesia and invasive techniques and requires minimal restraint of the animal. to recognize the acute pulmonary effect, the inspiratory volume of the animal was increased by continuous inhalation of a mixture containing 10% co2 in 18% o2 and 72% n2. once a plateau increase in inspiratory volume was reached, the aerosol to be studied was added to this mixture and its ef ... | 1985 | 3925716 |
| alterations in rat liver microsomal enzymes following exposure to carbaryl and malathion in combination. | | 1985 | 3929702 |
| graft rejection in a congenic panel of rats with defined immune response genes for class i antigens. ii. quantitative aspects of ir gene function in a full-haplotype mismatch. | previous studies have shown that the ir-gene-controlled rejection of rl tissues by c/u responder and non-rejection by c low responders does not extend to tissues expressing a full a haplotype mismatch. however, antibody responses and liver graft rejection are both defective in low responders, even across a full haplotype barrier. we have therefore used a titrated adoptive transfer assay to search for quantitative differences in the responsiveness of c and c/u animals to a organ grafts. we first ... | 1985 | 3931317 |
| enhanced responsiveness of the coronary blood vessel of the dog heart-lung preparation to pgi2 after chronic chemical sympathectomy. | chronic chemical denervation of the sympathetic nervous system with 6-oh dopamine resulted in an augmentation of the coronary blood flow (cbf) increase inherent in the canine heart-lung preparation, but not in an augmented accumulation of 6-keto pgf1 alpha in the circulating blood. in denervated hlp, 6-keto pgf1 alpha was lower and the vasodilator response to pgi2 larger at the start of the experiments. it was concluded that exaggerated increase in cbf was due to a potentiation of the vasodilato ... | 1985 | 3932732 |
| action of malotilate on reduced serum cholesterol level in rats with carbon tetrachloride-induced liver damage. | the mode of action of malotilate in normalizing serum cholesterol in hypocholesterolemic rats with fatty liver was examined by determination of biosynthesis, catabolism and excretion of cholesterol. fatty liver was produced by subcutaneous injection of ccl4 at the dose of 1 ml/kg into male rats (slc-sd) twice a week for 3 weeks. daily administration of malotilate (100 mg/kg) in rats with hypocholesterolemia resulted in a rapid normalization of lowered serum cholesterol. such a recovery of choles ... | 1985 | 3934440 |
| [chronic q fever infection (q fever endocarditis) with benign monoclonal gammopathy]. | | 1985 | 3935597 |
| labeling of the cytoplasmic domain of the influenza virus hemagglutinin with fluorescein reveals sites of interaction with membrane lipid bilayers. | the hemagglutinin (ha) glycoprotein of influenza virus was labeled in its cytoplasmic domain with fluorescein. reactive amino groups in the external domain were blocked by modification of the intact virus with the membrane-impermeable reagent isethionyl acetimidate. the ha was then solubilized with the detergent octyl glucoside, and the single lysine in the cytoplasmic domain was reacted with fluorescein isothiocyanate. this protocol resulted in the incorporation of 1.3 mol of fluorescein/mol of ... | 1985 | 3937554 |
| characterization of nif regulatory genes in rhodopseudomonas capsulata using lac gene fusions. | translational fusions of the escherichia coli laczya operon to rhodopseudomonas capsulata nif genes were obtained by using mini-mudii1734 [castilho et al., j. bacteriol. 158 (1984) 488-495] inserts into cloned fragments of r. capsulata dna. a lac fusion to the nifh gene, which encodes dinitrogenase reductase, was used to classify nif- mutations occurring in regulatory genes. nine mutations were unable to activate nifhdk transcription. the nine mutations define four nif regulatory genes. three of ... | 1985 | 3938422 |
| allopurinol hypersensitivity syndrome as a cause of hepatic fibrin-ring granulomas. | hepatic fibrin-ring granulomas were found in a 35-yr-old man who developed fever, myalgias, rash, eosinophilia, and abnormal liver function tests 4 wk after the beginning of allopurinol treatment. all clinical and biochemical abnormalities spontaneously resolved within 6 wk after cessation of therapy. there was no evidence for q fever or hodgkin's disease, which are the recognized causes of hepatic fibrin-ring granulomas. it is suggested that allopurinol hypersensitivity might be an additional c ... | 1986 | 3940244 |
| some issues raised on q fever study. | | 1986 | 3940459 |
| heart failure associated with infective endocarditis. a review of 40 cases. | thirty one (78%) of 40 consecutive patients (aged 13-79, mean 44 years) with infective endocarditis had congestive heart failure at presentation. twenty six (65%) had had rheumatic heart disease and 17 (43%) patients had prosthetic valves. eight (20%) patients had undergone dental procedures within three months of presentation. blood cultures were positive in only 22 (55%) of the patients. in nine (41%) of them streptococci of the viridans group were isolated and in seven (32%) patients endocard ... | 1986 | 3942652 |
| gene coding for the late 11,000-dalton polypeptide of the tian tan strain of vaccinia virus and its 5'-flanking region: nucleotide sequence. | a late gene coding for a major structural polypeptide of 11,000 daltons from the tian tan strain of vaccinia virus was cloned. a comparison of the sequences from tian tan and wr strains of vaccinia virus revealed that there were differences in four nucleotides and one amino acid and that there was little homology between the 5'-flanking sequences of the late and the early genes. however, substantial homology was detected between the 5'-flanking sequences of the late genes. | 1986 | 3944848 |
| acute, severe hepatitis due to coxiella burneti infection. | an unusual case of coxiella burneti infection is described in which the patient presented with an "acute abdomen" and marked clinical icterus. this case emphasizes that q-fever may appear as acute hepatitis and illustrates its "characteristic" histopathological lesions which have been recognized only recently. | 1986 | 3945204 |
| chemical modification of sheep-liver 6-phosphogluconate dehydrogenase by diethylpyrocarbonate. evidence for an essential histidine residue. | sheep liver 6-phosphogluconate dehydrogenase is shown to be inactivated by diethylpyrocarbonate in a biphasic manner at ph 6.0, 25 degrees c. after allowing for the hydrolysis of the reagent, rate constants of 56 m-1 s-1 and 11.0 m-1 s-1 were estimated for the two processes. the complete reactivation of partially inactivated enzyme by neutral hydroxylamine, the elimination of the possibility that modification of cysteine or tyrosine residues are responsible for inactivation, and the magnitudes o ... | 1986 | 3948881 |
| characterization of a phase i coxiella burnetii chloroform-methanol residue vaccine that induces active immunity against q fever in c57bl/10 scn mice. | the effect of phase i coxiella burnetii chloroform-methanol residue vaccine (cmrv) on the response of murine splenic lymphocytes to mitogenic and antigenic stimuli was evaluated in c57bl/10 scn endotoxinnonresponder mice with an in vitro lymphocyte proliferation assay. intraperitoneal injection of phase i cmrv resulted in antibody production against phases i and ii antigens. lymphocytes were responsive in vitro to concanavalin a, phytohemagglutinin, pokeweed mitogen, and specific recall antigens ... | 1986 | 3949384 |
| differential diagnosis of granulomatous meningoencephalomyelitis, distemper, and suppurative meningoencephalitis in the dog. | clinical differences were determined between granulomatous meningoencephalomyelitis, distemper, and suppurative meningoencephalitis in the dog. dogs with granulomatous meningoencephalomyelitis had "head" signs on examination, which progressed to profound caudal fossa abnormalities, changes in mental status, and tetraparesis. dogs with distemper had a gradual onset of posterior paresis; tetraparesis and occasional vestibular signs developed later in the course of disease. dogs with suppurative me ... | 1986 | 3949614 |
| differential diagnosis of granulomatous meningoencephalomyelitis, distemper, and suppurative meningoencephalitis in the dog. | clinical differences were determined between granulomatous meningoencephalomyelitis, distemper, and suppurative meningoencephalitis in the dog. dogs with granulomatous meningoencephalomyelitis had "head" signs on examination, which progressed to profound caudal fossa abnormalities, changes in mental status, and tetraparesis. dogs with distemper had a gradual onset of posterior paresis; tetraparesis and occasional vestibular signs developed later in the course of disease. dogs with suppurative me ... | 1986 | 3949614 |
| application of the "filter model" to a risk assessment for vinyl chloride. | the filter model was used to estimate thresholds for the induction of cancer from many dose-response sets for inhalation and ingestion exposure to vinyl chloride for rat and inhalation exposure for mouse. estimates for a variety of end-point combinations were log-normally distributed over about 2 decades from about 1 to 100 ppm for inhalation exposure to rat and 0.1 to 30 ppm for mouse. when the data is transformed to "dose" (milligrams per kilogram body weight per day), the estimates for inhala ... | 1986 | 3950992 |
| adiaspiromycosis in striped skunks in alberta, canada. | pulmonary adiaspiromycosis was diagnosed in seven of 25 striped skunks (mephitis mephitis) in east-central alberta. the infection varied from mild, where only microscopic lesions were seen, to severe, where gross lesions of grayish-white nodules were observed in the lung parenchyma. mild lesions were restricted to the lung, while severe lesions extended to the tracheobronchial and mediastinal lymph nodes. histologically, the lesions were characterized by a centrally located fungal spherule, surr ... | 1986 | 3951052 |
| antigenic variation in the phase i lipopolysaccharide of coxiella burnetii isolates. | coxiella burnetii isolates from a variety of clinical and geographical sources were screened for antigenic variation of lipopolysaccharides (lpss) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis coupled with silver staining or immunoblotting. all isolates from chronic q fever or other sources possessed a phase i-type lps. these lpss appeared to fall into three groups based on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile or on reactivity with rabbit anti-c. b ... | 1986 | 3957431 |
| chronic q fever endocarditis with massive splenomegaly in childhood. | two children with congenital heart disease developed persistent fever, anemia, and hepatosplenomegaly. both were shown to have intracardiac vegetations and evidence of infection with coxiella burnetti. thus, the same clinical manifestations of q fever may develop in both children and adults. | 1986 | 3958826 |
| pharmacokinetics and pharmacodynamics of subcutaneous naltrexone pellets in the rat. | subcutaneous implantation of naltrexone pellets is a standard method of producing chronic blockade of opioid receptors. in the present experiments, rats were treated with two, 30-mg naltrexone pellets and the pharmacokinetics and pharmacodynamics examined. this dosing method produced high plasma naltrexone levels (350 ng/ml) by 1 hr which declined over an implant period of 192 hr (24 ng/ml). approximately 40% of the systemically available naltrexone (15.6 mg) was released in the first 24 hr. a t ... | 1986 | 3958962 |
| pharmacokinetics and pharmacodynamics of subcutaneous naltrexone pellets in the rat. | subcutaneous implantation of naltrexone pellets is a standard method of producing chronic blockade of opioid receptors. in the present experiments, rats were treated with two, 30-mg naltrexone pellets and the pharmacokinetics and pharmacodynamics examined. this dosing method produced high plasma naltrexone levels (350 ng/ml) by 1 hr which declined over an implant period of 192 hr (24 ng/ml). approximately 40% of the systemically available naltrexone (15.6 mg) was released in the first 24 hr. a t ... | 1986 | 3958962 |
| [the systematic evaluation of medical procedures. apropos of the effectiveness of parallel medicine]. | | 1986 | 3961360 |
| synthesis of three 3-c-hydroxymethylpentoses with the d-ribo-, d-xylo- and l-lyxo-configurations. identification of the latter with a monosaccharide isolated from phase i coxiella burnetii lipopolysaccharide. | three 3-c-hydroxymethylpentoses with the d-ribo-, d-xylo and l-lyxo-configurations, were synthesised via nitromethane addition for the first two and 1,3-dithiane addition for the last one, to appropriate 3-ulose derivatives. 3-c-hydroxy-methyl-l-lyxose is identical with a monosaccharide component previously isolated from hydrolysates of the phase i coxiella burnetii lipopolysaccharide. | 1986 | 3962550 |
| suppression of in vitro lymphocyte proliferation in c57bl/10 scn mice vaccinated with phase i coxiella burnetii. | the effect of inactivated phase i and phase ii coxiella burnetii whole cell vaccine (wcv) on the response of murine spleen cells to mitogenic and antigenic stimuli was evaluated in c57bl/10 scn endotoxin nonresponder mice with an in vitro lymphocyte proliferation assay. intraperitoneal injection of phase i wcv into mice resulted in marked and persistent suppression of the proliferative response of spleen cells to concanavalin a, phytohemagglutinin, and pokeweed mitogen. this response was time an ... | 1985 | 3965392 |
| cell cycle distribution patterns and generation times of l929 fibroblast cells persistently infected with coxiella burnetii. | coxiella burnetii established a persistent infection of various cell lines including l929 mouse fibroblasts. although the basis for such persistence is unknown, the phenomenon does require continual growth, proliferation, and maintenance of viability of the host cells. we examined the effect of short- and long-term infection on the host cell's generation time and cell cycle. flow cytometric studies of actively growing normal and infected cells stained with mithramycin or propidium iodide reveale ... | 1985 | 3967922 |
| regulated biosynthesis and divergent metabolism of three forms of hepatic apolipoprotein b in the rat. | studies using rat livers perfused with recycled, serum-containing medium plus [3h]leucine revealed that secreted vldl contain three forms of apolipoprotein b (apob), b-48, b-95, and b-100, all synthesized by the liver. the b-48/(b-95 + b-100) [3h]leucine incorporation ratio ranged from 0.22 to 3.25 with livers of rats fed different diets, and the ratio was positively correlated with the triglyceride secretion rate in most of the livers. generally, as more triglyceride was secreted, a greater pro ... | 1985 | 3973514 |
| [hepatic form of q fever. apropos of a case]. | | 1985 | 3975463 |
| [humoral immune response of goats, rabbits and guinea pigs following vaccination with a q fever vaccine]. | | 1985 | 3985935 |
| activation of branched-chain alpha-ketoacid dehydrogenase complex by alpha-chloroisocaproate in normal and enzyme-deficient fibroblasts. | a method has been developed for the activation of the branched-chain alpha-ketoacid dehydrogenase complex by alpha-chloroisocaproate, an inhibitor of branched-chain alpha-ketoacid dehydrogenase kinase in human cultured skin fibroblasts. the enzyme could be activated by pretreating the cells with alpha-chloroisocaproate before they were disrupted for measurement of the activity. after this treatment, the activity was 2- to 3-fold that of untreated cells (24.8-81.4 pmol/min per mg protein). the en ... | 1985 | 3987057 |
| lipopolysaccharide variation in coxiella burnetti: intrastrain heterogeneity in structure and antigenicity. | we isolated lipopolysaccharides (lpss) from phase variants of coxiella burnetii nine mile and compared the isolated lps and c. burnetii cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. the lpss were found to be the predominant component which varied structurally and antigenically between virulent phase i and avirulent phase ii. a comparison of techniques historically used to extract the phase i antigenic component revealed that the aqueous phase of phenol-wa ... | 1985 | 3988339 |
| [subacute brucellosis with simultaneous seroconversion against coxiella burnetii]. | | 1985 | 3990426 |
| [infection prevention in animal husbandry. a contribution to the improvement of the sanitary consumer protection]. | the scientific and organizational development of an effective prophylaxis against infections in animal husbandry results from the fact that many zoonoses, like salmonellosis, campylobacteriosis, toxoplasmosis, leptospirosis, listeriosis, rickettsiosis (q-fever) and cysticercosis as well as certain important virus infections with regard to meat hygiene cannot be detected during official ante- and postmortem inspection. the cause of these infections is clinically inapparent and leaves no pathologi ... | 1985 | 3993255 |
| myocardial blood flow during cardiac tamponade in dogs with coronary occlusion: effects of isoproterenol. | to determine the effects of cardiac tamponade in dogs with ligation of the left anterior descending coronary artery, fluid was introduced into the pericardial space to raise right and left atrial and pericardial pressures, first to 7 to 9 mm hg and then to 11 to 12 mm hg. normal and ischemic myocardial blood flow fell approximately 20% to 25% during mild tamponade (1.27 +/- 0.16 to 1.00 +/- 0.06 ml/min/gm and 0.52 +/- 0.12 to 0.39 +/- 0.08 ml/min/gm, respectively) and by 50% during moderate tamp ... | 1985 | 3993513 |
| q fever serologic surveillance program for sheep and goats at a research animal facility. | since q fever is a potential risk to personnel working with small ruminants, the serologic status of sheep and goats received at a medical school animal facility for research was evaluated. a total of 104 sheep and 102 goats were subjected to blood sample-collection procedures on arrival, as well as after a 2-week quarantine period, and the sera were tested for q fever specific antibodies by complement-fixation (cf) and microagglutination (ma) tests. the results from the 2 tests were compared an ... | 1985 | 3994098 |
| contact urticaria from diethyl fumarate. | the contact urticariagenic properties of diethyl fumarate were studied using the guinea pig ear swelling assay and by open application on the human upper back skin. diethyl fumarate caused non-immunologic contact urticaria in both human and guinea pig skin, and the reactions exhibited similar dose dependency and timing of maximal response. | 1985 | 3995941 |
| 3-c-branched aldoses in lipopolysaccharide of phase i coxiella burnetii and their role as immunodominant factors. | mild acid hydrolysis with 1% acetic acid (100 degrees c, 15-60 min) of lipopolysaccharide (lps) isolated from coxiella burnetii phase i cells leads to a drastic decrease in its serological reactivity as shown by the passive hemolysis test. this decrease in reactivity occurs parallel or even prior to the cleavage of lps into free lipid a and the polysaccharide moiety. during this mild hydrolysis two unusual sugars (x and y) are released from the lps, which were obtained in pure state by thin-laye ... | 1985 | 3996391 |
| ribosomal protein phosphorylation induced during q fever or by lipopolysaccharide: in vitro translation is stimulated by infected liver ribosomes. | q fever, as well as the lipopolysaccharide prepared from the rickettsial agent coxiella burnetii, stimulates the phosphorylation of guinea pig liver ribosomal protein s6. in vitro mrna and ribosome-dependent rabbit reticulocyte lysate translation systems reconstituted with ribosomes and mrnas from infected animal livers were more active than those with mrnas and ribosomes from uninfected animals. treatment of ribosomes with a ribosomal supernatant phosphatase reduced the in vitro translation act ... | 1985 | 3997241 |
| [enzyme-linked immunosorbent assay (elisa) for the demonstration of igg1, igg2 and igm antibodies in bovine q fever infection]. | by application of igg1-, igg2-, and igm-specific conjugates in an enzyme-linked immunosorbent assay (elisa), dominance of igg1 in natural q-fever infections of cattle could be demonstrated. in contrast, vaccination with an inactivated q-fever vaccine predominantly induced igg2 antibodies. complement fixing activity was detected in positive sera (inactivated at 56 degrees c) in the igg1 fraction only. therefore, with serum samples containing exclusively igm (10%), or igg2 (4%), a serodiagnosis co ... | 1985 | 4002933 |
| high-performance liquid chromatographic method for quantification of cyproheptadine in serum or plasma. | | 1985 | 4008588 |
| [trial of an erythrocyte antigenic diagnostic agent for detecting antibodies to coxiella burnetii]. | a new method for the preparation of q-fever erythrocyte antigenic diagnosticum, adaptable to large-scale production, has been developed, and the diagnosticum thus obtained has been found to be highly specific and sensitive. the combined use of the complement fixation and passive hemagglutination tests enhances the effectiveness of the serological study, as it not only ensures a more complete detection of antibodies to c. burnetii, phase i, in humans and cattle, but also gives more precise indica ... | 1985 | 4013575 |
| [clinico-epidemiological characteristics of q fever in the carpathian region]. | the study of the specific epidemiological and clinical features of q fever revealed the existence of an active focus of infection among humans due to their contacts with agricultural animals in one of the districts of the region. the focus was manifested by group morbidity among the cattle-tending personnel of a dairy farm. the source of this infection was cattle. the infection was transferred mainly through the air. the disease took a moderately severe course. the study of the rickettsial conta ... | 1985 | 4013578 |
| q fever as a possible cause of bilateral optic neuritis. | a 59-year-old farmer developed a bilateral optic neuritis in the course of an infection with coxiella burnetii (q fever), resulting in a lasting unilateral blindness. a complete medical (including neurological) evaluation gave no evidence of other possible causes. a possible source of infection was cattle. | 1985 | 4016056 |
| the role of shed fc receptor in the regulation of lymphocyte response to phytohaemagglutinin (pha). | supernatants from 18-hr cultures of human peripheral blood polymorphonuclear cells (pmn) and adherent cells (ac) inhibited the binding of erythrocyte-antibody complexes (ea) by pmn. the inhibitory activity was lost upon absorption on igg and fc, but not on the f(ab')2 fragment of igg. igg-binding material (shed fc receptor--fcr) was reutilized by pmn and ac which had lost their own fcr in vitro. reutilization was independent of the type of cells from which fcr originated. the fcr-containing mate ... | 1985 | 4018846 |
| changes in circulating immune complex concentrations and antibody titres during treatment of q fever endocarditis. | serum samples from 20 patients with q fever endocarditis were tested for the presence of circulating immune complexes to see whether their concentrations correlated with antibody titres during treatment and whether they could be used to monitor the response to antimicrobial treatment. circulating immune complexes were found in all 20 patients. the concentrations in 15 patients correlated with either or both of the q fever phase 1 and phase 2 antibody titres obtained during treatment. in the othe ... | 1985 | 4019797 |
| hypertension during chronic peripheral and central infusion of angiotensin iii. | recent studies have suggested that the angiotensin ii (ang ii) metabolite [des-asp]ang ii (ang iii) may be nearly equipotent with the parent compound in causing several acute neural responses known to be stimulated by angiotensin peptides (i.e., drinking, augmentation of sympathetic neurotransmission, and centrally mediated pressor responses). because neural actions of ang ii are thought to contribute importantly to the ability of this hormone to cause chronic hypertension, the purpose of the pr ... | 1985 | 4025541 |
| q-fever control in cyprus--recent progress. | | 1985 | 4027560 |
| effects of neonatal castration or treatment with dihydrotestosterone on numbers of neurons in the rat superior cervical sympathetic ganglion. | there is a normal sexual dimorphism in the number of neurons in the rat superior cervical sympathetic ganglion (scg), with adult males having more neurons in this ganglion than females. we confirm this finding here, report that this sex difference is not present at birth and that neonatal castration of males reduces the adult sex difference. these results indicate that perinatal levels of circulating testicular hormones play a role in regulating the numbers of neurons in the scg. treatment of ne ... | 1985 | 4027673 |
| reentrant ventricular arrhythmias in the late myocardial infarction period in the dog. 13. correlation of activation and refractory maps. | isochronal maps of ventricular activation were analyzed in dogs 3-5 days after ligation of the left anterior descending coronary artery utilizing a 64-channel multiplexer. isochronal maps of the effective refractory period were determined from 62 epicardial sites and correlated with the activation maps. the ischemia occurring in the surviving epicardial layer prolonged refractoriness in a spatially nonuniform manner. the resulting pattern of refractoriness on the epicardial surface resembled con ... | 1985 | 4028346 |