| [bacterial ileocecitis: a "new" disease]. | in cases of clinical suspicion of an acute appendicitis sonography uncovers another disease as the real cause of the symptoms in about one-quarter of such cases. bacterial ileocaecitis is most frequently diagnosed (11.6% of n = 786). in special bacteriological stool cultures, yersinia enterocolitica and campylobacter jejuni were identified, whereas salmonella enteritidis was a rarer finding. the typical sonographic manifestation of bacterial ileocaecitis compared against crohn's disease of the i ... | 1991 | 1796286 |
| protection against the lethal effect and arthritogenic capacity of yersinia enterocolitica serovar o:3 for mice. | protection against the intravenous lethal effect of yersinia enterocolitica serovar o:3 for mice can be achieved by oral immunization with bacteria expressing the o:3 lipopolysaccharide (lps). under similar experimental conditions, ca(2+)-dependent cells are more protective than their ca(2+)-independent counterparts, live vaccines are more efficacious than killed ones, and parenteral immunization is more efficient than the oral route. antibodies induced by enzyme-treated lps from an o:3 strain a ... | 1991 | 1797050 |
| virulence of yersinia spp. strains isolated from non-human sources. | 1. strains of yersinia enterocolitica, y. intermedia, y. frederiksenii and y. kristensenii from non-human sources were examined for virulence factors. four of these strains were positive for autoagglutination, three were calcium dependent at 37 degrees c, three produced lipase, five had the ability to bind congo red, six had pyrazinamidase activity, and two had a 42 mda plasmid. 2. one strain (y. enterocolitica 0:5) was lethal for mice and had the ability to invade guinea pig eyes and hela cells ... | 1991 | 1797265 |
| [secondary immunodeficiency in pseudotuberculosis in man and in experiment]. | surgical material from 55 children, from 2 to 15 years of age with an abdominal form of pseudotuberculosis (appendix, lymph nodes, ileum) and the immunogenesis organs (lymphoid tissue of the small intestine, lymph nodes, appendix, spleen and thymus) of 277 rabbits infected with virulent strains of the yersinia pseudotuberculosis i serovar at the dose of 10(8) bacterial bodies were investigated. the results testify to the disturbance of all parts of the immuno-phagocytic system in pseudotuberculo ... | 1991 | 1799314 |
| antibacterial activity of cefpodoxime in comparison with cefixime, cefdinir, cefetamet, ceftibuten, loracarbef, cefprozil, bay 3522, cefuroxime, cefaclor and cefadroxil. | the new oral cephalosporins cefpodoxime, cefixime, cefdinir, cefetamet and ceftibuten demonstrate enhanced activity against enterobacteriaceae susceptible to the established compounds as well (e.g. cefuroxime, cefaclor, cefadroxil). in addition, cefpodoxime, cefixime, cefdinir, cefetamet and ceftibuten include in their spectrum species hitherto resistant to oral cephalosporins (proteus vulgaris, providencia spp., yersinia enterocolitica). besides, the majority of these compounds demonstrate rele ... | 1991 | 1800377 |
| a study of the bacterial and parasitic causes of acute diarrhoea in northern jordan. | the prevalence of bacterial and parasitic diarrhoeagenic agents in 200 patients suffering from acute diarrhoea and reporting to hospitals and health centres in northern jordan, and in controls was determined. one or more bacterial or parasitic enteropathogens was isolated from 79 patients (39.5%). prevalence rates for these pathogens was as follows: enterotoxigenic escherichia coli, 9%; enteropathogenic e. coli, 9%; salmonella spp. 7%; campylobacter spp, 5.5%; yersinia enterocolitica, 4.5%; shig ... | 1991 | 1800560 |
| [functional state of thrombocytes in yersinia infection]. | as many as 94 patients with yersiniosis were examined for the morphofunctional status of platelets (aggregation, disaggregation, endo-exocytosis and platelet forms). the data indicate that platelet from patients with yersiniosis possess a high ability for aggregation and a low ability for disaggregation. according to the experimental and clinical data, emoxipine can restore disturbed function of human platelets. | 1991 | 1801465 |
| [the epidemiological patterns of pseudotuberculosis in khabarovsk territory]. | materials on the epidemiological characterization of pseudotuberculosis at the khabarovsk territory taken as a whole and in individual settlements, as well as on the evaluation of epizootic situation in natural foci of infection and foci created by human activities, are presented. the results of laboratory studies of vegetables, washings from environmental objects, soil specimens, material taken from rodents are demonstrated and the characterization of the virulent properties of isolated yersini ... | 1991 | 1801483 |
| [the heterogeneity of the alveolar macrophages of guinea pigs immunized subcutaneously with a vaccinal strain of yersinia pestis]. | the study of alveolar macrophages obtained from guinea pigs at different periods after the subcutaneous immunization with y. pestis vaccine strain has revealed the heterogeneity of these macrophages in their bactericidal activity with respect to y. pestis and in their resistance to the cytopathic action of this microorganism, as well as in the content of lysosomes and in the expression of receptors on the cell surface. the study has shown that subcutaneous immunization does not lead to the redis ... | 1991 | 1801486 |
| [the use of immunoenzyme analysis in the laboratory diagnosis of plague]. | the possibility of using the enzyme immunoassay (eia) for the early diagnosis of pneumonic plague was studied in experiments on monkeys. eia was shown to be more effective than the passive hemagglutination test. the diagnostic value of blood serum samples was found to be higher than that of nasopharyngeal mucus samples taken from the sick animals. the conclusion on the suitability of eia for the early laboratory diagnosis of this disease was made. | 1991 | 1801488 |
| [an immunoenzyme test system for the detection of antibodies to campylobacter]. | an enzyme immunoassay system for the detection of antibodies to bacteria of the genus campylobacter in human blood serum has been developed. the system is based on the use of ethanol-treated c. jejuni and c. coli whole cells as antigen. the study of sera obtained from healthy donors in this assay has made it possible to establish the value of the tentative diagnostic titer: 320. | 1991 | 1801490 |
| [the adhesive properties of bacteria of intestinal origin]. | differences between strains of nonpathogenic escherichia and lactobacilli, as well as some pathogenic bacteria of enteric origin (escherichia, shigella, campylobacter), in their capacity to adhesion to rat enteric and colonic cells have been shown in vitro. the strains under study have been found to possess more pronounced adhesiveness with respect to colonic cells, which is indicative of their higher receptive capacity in comparison with enteric cells. in the absence of normal microflora lactob ... | 1991 | 1801491 |
| [constructing a test system for the identification of plague microbe based on genetic probes]. | dna probes for detection of the plague agent yersinia pestis were made on a basis of its three typical extrachromosomal replicons. the recombinant plasmid pbs2 including pbr327 vector and salgi-bspri fragment of the plasmid pfra was constructed. the above fragment is connected with synthesis of y. pestis capsular antigen and it is a 400 bp species-specific dna probe called f1 which is suitable for identification of y. pestis species that bears the 60 mdal plasmid. the dna probes called p1 was ma ... | 1991 | 1802792 |
| characterization of smooth brucella lipopolysaccharides and polysaccharides by monoclonal antibodies. | two mouse monoclonal antibodies (mab) generated to the m antigen of brucella melitensis 16m were analysed. binding profiles of both monoclonals were established by a competitive enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides, o polysaccharides and native hapten polysaccharides from b. melitensis 16m, b. abortus 544 and yersinia enterocolitica o:9. using this assay, significant differences in the reactivity of both antibodies were found with a and m antigens from b ... | 1991 | 1805311 |
| what is the diagnostic value of beta-d-glucuronidase (bdg) activity of bacteria using fluorocult ecd agar for their cultivation? | a total of 1510 strains from 15 genera of gram-negative and gram-positive bacteria were studied. more than 94% of 327 escherichia coli strains showed beta-d-glucuronidase (bdg) activity. seventeen serotypes from 170 e. coli o serogroup representatives were negative. relationship between the existence of bdg positive and negative e. coli strains in the same serogroup or serotype has not been observed. the rate of bdg positivity was 42% among salmonella arizonae strains and 42.2% among shigella st ... | 1991 | 1805501 |
| yersinia enterocolitica infection of a prosthetic knee joint. | yersinia enterocolitica is a very common organism which is mainly associated with gastrointestinal symptoms. it has not previously been associated with early or late infection of a prosthetic joint replacement. we report a case of late infection, seven years after an uncomplicated knee replacement. this case demonstrates the ability of the organism to breach the joint capsule. there may well be an inherent under-diagnosis of y. enterocolitica in infected prostheses due to the difficulty in the p ... | 1991 | 1805923 |
| [anatomoclinical conference at the pitié-salpêtrière hospital. psoas abscess in a 17-year-old male]. | | 1991 | 1807181 |
| adaptational changes of fatty acid composition and the physical state of membrane lipids following the change of growth temperature in yersinia enterocolitica. | yersinia enterocolitica is capable of growing in a broad range of temperatures from 4 to 45 c. how this organism alters its membrane lipids in response to the change of growth temperature is very interesting. the fatty acids of membrane lipids of cells cultured at 5, 15, 25 and 37 c were analyzed and the physical states of these membrane lipids were characterized. the major phospholipids of this bacterium were phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, lysophosphatidylglycerol ... | 1991 | 1808462 |
| [connection of outer membrane protein composition in yersinia pestis cells with intrinsic plasmids]. | a set of isogenic derivatives of yersinia pestis ev strain was obtained including the variants harbouring the different compositions of yersinia own plasmids. the protein profiles of outer membranes of the set of strains were defined. the polyacrylamide gel electrophoresis has shown the small 6.1 md plasmid to code an outer membrane protein with mol mass 29 kda, different from pesticin i, while the heavy 60.0 md plasmid encodes the 15-16 kda polypeptide different from monomers of f1 and t-antige ... | 1991 | 1808507 |
| [alternative localization of the determinant of pathogenicity coded by the yersinia pseudotuberculosis pvm82 plasmid]. | the chromosomal dna regions in yersinia pseudotuberculosis strains occur that are homologous to 25 md dna segment of the plasmid pvm82 encoding the bacterial capability of immunosuppression. the character of the chromosomal dna regions dispersion reacting with the 25 md segment probes is different in epidemiologically hazardous and nonvirulent strains of yersinia pseudotuberculosis. the specific dna regions occur as well as identical ones. the suppression of antibody formation to a number of mai ... | 1991 | 1808508 |
| [new plasmidovars of yersinia pestis isolated in mongolia]. | the plasmid spectres of 122 strains of yersinia pestis isolated in mongolia from patients, wild mammals and arthropods were studied. the populations of three plasmidovars of yersinia pestis were found to be circulating in the natural foci of plague in mongolia. the first plasmidovar harbours three plasmids with mol masses 6, 47, 65 md. the second and third plasmidovars contain the plasmids with mol masses 6, 16, 47, 65 md and 8, 47, 75-80 md. | 1991 | 1808509 |
| [the clinico-allergic indices of yersiniosis patients]. | eighty patients with yersiniosis (40 with intestinal yersiniosis, 40 with pseudotuberculosis) were examined for clinical and allergic characteristics (collection of allergological histories, identification of the most common allergic symptoms of the disease and determination of the index of mast cell degranulation). according to the data obtained, the patients with yersiniosis are admitted to the hospital with an aggravated premorbid condition in the form of body allergization, in whose origin b ... | 1991 | 1810058 |
| [the characteristics of the course of yersinia hepatitis in cholelithiasis patients]. | the paper is concerned with a clinical description of yersiniosis in 12 women who suffered from manifest or uneventful cholelithiasis. the disease started by an attack of biliary colic. the clinical symptomatology was noticed to run in stages during which the signs of calculous cholecystitis vanished while the symptoms of hepatitis persisted. in the acute period, the conservative treatment of the patients was carried out by the infectionist jointly with the surgeon, followed by dispensary observ ... | 1991 | 1810059 |
| [the characteristics of pseudotuberculosis with joint involvement in the presence of chronic opisthorchiasis]. | a study was made of the clinical picture, certain laboratory and immunological findings in pseudotuberculosis patients with involved joints in the presence of opisthorchiasis invasion. the patients suffering from pseudotuberculosis and chronic opisthorchiasis frequently demonstrated icteric sclera, erythema nodosum, skin itch, tenderness in the right hypochondrium, hepatomegaly, liquid stool, leukopenia, eosinophilia, hyperbilirubinemia, activation of aminotransferases. later they showed the dia ... | 1991 | 1810061 |
| causative role of yersinia and other enteric pathogens in the appendicular syndrome. | in 2,861 consecutive patients undergoing appendicectomy for clinically suspected appendicitis an enteric pathogen was isolated from the appendix in almost 7% using an optimal combination of culture media. the pathogenic yersinia enterocolitica serotypes 03 and 09 predominated (3.6%), followed by campylobacter and nontyphoid salmonella. the same pathogen was isolated from the stool in 72.5% of patients with a culture-positive appendix and in 84.1% of those positive for a pathogenic yersinia. conv ... | 1991 | 1810725 |
| first isolation of yersinia enterocolitica serotype o:8 in japan. | a 4-year-old japanese boy was infected with yersinia enterocolitica serotype o:8, h:befiv, biotype 1b, phage type xz, restriction endonuclease analysis of plasmid dna type b, restriction endonuclease analysis of chromosomal dna type 8. he presented with acute gastroenteritis with elevated body temperature (40 degrees c), rigor, and shivers. the diagnosis was septicemia. this is apparently the first report of this serotype from a human infection outside north america. | 1991 | 1812854 |
| [comparative analysis of the 5'-terminal nucleotide sequence and central domains of 16s rrna of yersinia pestis and escherichia coli]. | partial nucleotide sequence of 16s rrna (16-989 nn.) of plague agent (yersinia pestis) was determined after sequencing of cloned cdna fragments. the comparison of y. pestis 16s rrna sequence with that of e. coli shows a number of point sequence variation due to base changes. the base changes are found in 16s rrna secondary structure regions that are localized on the surface of 30s ribosome subunit (hairpins 6 and 18) as well as in the regions that bind the proteins s8, s15, s16 and s20. these pr ... | 1991 | 1813805 |
| [bacteria of the genus yersinia in chickens for human consumption and in balanced bird food]. | yersinia was investigated in 50 skin samples of chicken carcasses from retail shops and 65 samples of balanced food for domestic fowl. enrichments were performed in saline phosphate buffer 0.067 m, ph 7.6 and post-enriched in 0.5% koh. subcultures were performed in salmonella-shigella agar and macconkey agar. isolates were identified through biochemical, serological and lysotyping methods. the following biovar (b), serovar (o) and phagovar (lis) were isolated from chickens: y. enterocolitica (fi ... | 1991 | 1815265 |
| beta-d-glucuronidase (bdg) activity of gram-negative bacteria. | bdg is an inducible enzyme that is encoded by the uida gene in escherichia coli. genetic sequences of this gene are present in most if not all e. coli strains regardless of the bdg phenotype. expression of bdg activity can be influenced by lactose-induced catabolite repression or genetic mutations. salmonella, shigella and yersinia strains frequently exhibit positive bdg reaction. bdg activity of strains belonging to genus edwardsiella, serratia, yersinia, vibrio, erwinia, alcaligenes, acinetoba ... | 1991 | 1817425 |
| [oral infection by y. enterocolitica 0:8. studies of immunization in a murine model]. | the evaluation and mechanism of protection against y. enterocolitica o:8 using different immunization schedules. we immunize 3 groups of 20 mice each against an oral lethal dose of y. enterocolitica o:8 using: 1) whole cells, 2) hyperimmune sera, and 3) lps and common antigen. animals immunized using whole live cells that produce common antigen (o:3 +v and ip614) developed the highest degree of protection, with an overall survival rate of 70% and 60% respectively. similar results were obtained u ... | 1991 | 1822150 |
| [specific proteolysis by fibrinolysin-coagulase from yersinia pestis of yersinia pseudotuberculosis outer membrane proteins coded by the ca(2+)-dependence plasmid]. | the pesticinogenicity 9.5 kb plasmid from yersinia pestis strain ev76 has been marked by the kanamycin phosphotransferase gene inserted into psti site and designated pp3. the obtained plasmid pp3 determines the synthesis of 45 kd pesticin, alpha and beta-forms of fibrinolysin coagulase (37 and 35 kd) and the 29, 19 and 13 kd proteins in escherichia coli mini cells. when transferred into yersinia pseudotuberculosis strain 6933 the plasmid causes the proteolysis of outer membrane proteins. the 150 ... | 1991 | 1827173 |
| [infectious risks in patients with renal failure on hemodialysis. importance of iron overload and deferoxamine]. | | 1991 | 1828579 |
| [axillary lymphadenitis due to yersinia pseudotuberculosis]. | | 1991 | 1830152 |
| yersinia pseudotuberculosis isolation from cockatoo. | | 1991 | 1830763 |
| survival of yersinia enterocolitica in soil and water. | yersinia enterocolitica o3, o4, o5a, o5b, o6, o6, 30, o9 and o13 and yersinia intermedia were examined to clarify their survival in natural soil, river water and well water. the o3 strain disappeared most rapidly from soil at both temperature of 4 degrees c and 20 degrees c and from river water at 20 degrees c. although the o5b and o9 strains disappeared before the o3 strain did from river water at 4 degrees c, the o9 strain survived as long as non-virulent strains did in soil at 4 degrees c. th ... | 1991 | 1830776 |
| [pseudotuberculosis in the krasnodar territory. the clinico-epidemiological characteristics of its morbidity and the biological properties of its strains]. | group cases of pseudotuberculosis, first registered at health-restoring institutions of the health resort of gelendzhik (the krasnodar territory), are described. the etiology of these cases of the disease was established on the basis of clinico-epidemiological data, specific seroconversion in 66% of the examined patients and the isolation of yersinia pseudotuberculosis strains from the feces of four patients. the combination of signs observed in the isolated strains, viz. the presence of plasmid ... | 1991 | 1831948 |
| evidence that yersinia ruckeri possesses a high affinity iron uptake system. | this work represents the first evidence of the presence of an iron uptake system siderophore mediated in the bacterial fish pathogen yersinia ruckeri. a group of 20 strains representative of this species, with different serotype and origin were examined. all of them were able to grow at high concentrations (from 0.7 to 1.1 mm) of the iron chelator edda. although the y. ruckeri isolates failed to cross-feed the indicator strains for enterobactin and aerobactin production, the chemical tests revea ... | 1991 | 1832113 |
| [prevalence of anti-yersinia enterocolitica antibodies in an open population of the city of rome]. | this work shows the results of a seroepidemiological study of a group of 691 apparently healthy individuals: 246 males and 445 females from a rome out-patient consulting center. 72 individuals (10.42%) were found positive with an antibody titer from 1:20 to 1:40. the percentages regarding the sexes and also those regarding the age groups did not show significant differences. the results of the research demonstrate a limited diffusion of yersinia enterocolitica infection in the area under scrutin ... | 1991 | 1834405 |
| [yersinia enterocolitica septicemia associated with idiopathic hemochromatosis and deferoxamine therapy. a case]. | yersinia enterocolitica septicaemia is an infrequent condition. we report a case in which septicaemia developed after an infusion of desferrioxamine in a man with idiopathic haemochromatosis. according to the literature, the association of idiopathic haemochromatosis, liver disease and desferrioxamine therapy is extremely favourable to the development of yersinia septicaemia because iron intake is known to enhance the virulence of this organism, and desferrioxamine, acting like a chelator, enabl ... | 1991 | 1835044 |
| [severe yersinia enterocolitica infections in kidney failure patients under dialysis]. | | 1991 | 1836634 |
| [synthesis, secretion, and proteolytic degradation of diphtheria toxin in escherichia coli]. | the recombinant plasmids have been constructed encoding the synthesis of a full-sized diphtheria toxin from its own or pr, pl-promoters of bacteriophage lambda in escherichia coli cells. the high level constitutive synthesis of toxin results in slow cell growth and plasmid elimination. the toxin was mainly detected in the periplasm, partially in the membrane and to a less extent in the cytoplasm and culturing medium. the dimeric form of toxin was found in the cytoplasm. participation of toxin b- ... | 1991 | 1836836 |
| the yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the alpha 5 beta 1 integrin receptor. | the yersinia pseudotuberculosis invasin protein promotes bacterial penetration into mammalian cells by binding to several beta 1 chain integrins. we show here that proteins containing the cell-binding domain of invasin bind to the fibronectin receptor alpha 5 beta 1 isolated from human placenta and immobilized on a filter membrane. two forms of the receptor, each having a molecular weight of about 290,000, were immunodepleted by monoclonal antibodies specific for the beta 1 subunit or the alpha ... | 1991 | 1837020 |
| [fleas of the eastern kyzyl kum and their epizootic significance]. | the fauna of fleas in the east kisil-kum includes 24 species. different physicogeographic regions of sands are characterized by different spectra and number of species of fleas. the greatest share in collections is composed by xenopsylla gerbilli caspica--the parasite of rhombomys opimus. in autumn the other parasite of this rodent, coptopsylla lamellifer, is a mass species. all the most significant and long changes in the number of these species are associated with changes in the density of the ... | 1991 | 1839698 |
| [the hepatitis form of yersiniosis]. | | 1991 | 1839752 |
| genetic analysis of homology between the virulence plasmids of salmonella dublin and yersinia pseudotuberculosis. | two segments within the virulence region of salmonella dublin plasmid psdl2 that were homologous to regions on yersinia pseudotuberculosis plasmid pib1 were located with regard to the four known genes (vsda, vsdb, vsdc, and vsdd) of psdl2. one segment mapped upstream of vsda within an insertion element related to is630 of shigella sonnei; the second was confined to a 45-bp sequence containing an inverted repeat between vsdc and vsdd. on pib1, both areas were located in an intergenic region upstr ... | 1991 | 1840573 |
| the use of dacron plates for dot enzyme-linked immunosorbent assay (dot-elisa). | dacron (polyethylenetherephthalate) is proposed as a matrix for dot-elisa procedures, as an alternative to nitrocellulose. plates of dacron were partially hydrazinolyzed and hydrazide groups introduced were converted to azide groups. the derivative dacron-antigen was covalently linked on to the plates through these azide groups. the derivative dacron-antigen was exaustively washed according to crook and antigen was still fixed onto the plates. protein f1a purified from yersinia pestis was used a ... | 1991 | 1842438 |
| yersinia pseudotuberculosis infection as a cause of arthritis in children. | | 1991 | 1845401 |
| a salmonella typhimurium virulence protein is similar to a yersinia enterocolitica invasion protein and a bacteriophage lambda outer membrane protein. | the phop-phoq-regulated pagc locus is essential for full virulence and survival within macrophages of salmonella typhimurium. the protein product, dna sequence, and transcript of pagc were determined. the pagc locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell invasion protein of yersinia enterocolitica and the lom-encoded protein of bacteriophage lambda. the similarity of pagc and ail to lom leads us to hypothesize that lom is a virulence p ... | 1991 | 1846140 |
| influence of interleukin 6 on the function of secondary cultures of human thyrocytes. | interleukin 6 has been suggested as second mediator of the effects of interleukin 1 in some cell systems. interleukin 1 has previously been shown to inhibit the function of human thyrocytes in secondary cultures. we have therefore studied the influence of interleukin 6 (10(-1)-5.10(7) u/l) on the function of thyroid cells. recombinant interleukin 6 slightly inhibited the production of camp, but failed to influence the production of thyroglobulin or the dna content. endotoxins (lipopolysaccharide ... | 1991 | 1851359 |
| screening blood donors for gastrointestinal illness: a strategy to eliminate carriers of yersinia enterocolitica. | recent reports of fatal transfusion-associated yersinia enterocolitica sepsis prompted a study of the feasibility of adding a question to the routine donor health history as a method of reducing this risk. in three american red cross blood centers, 11,323 donors were asked one of two questions about gastrointestinal symptoms during their health history screenings. affirmative responses were obtained from 0.6 or 4.0 percent of the donors, depending on how the question was asked. in one center, mo ... | 1991 | 1853442 |
| slaughter pigs and pork as a source of human pathogenic yersinia enterocolitica. | pathogenic yersinia enterocolitica strains (serogroups 0:3;0:9 and 0:5.27) were isolated from 36 (42%) of 86 porcine tonsils, 8 (20%) of 40 tongues, 17 (17%) of 100 rectal swabs and from 4 (1%) of 400 pork samples. pathogenic yersinia strains were not isolated from samples of 210 pig carcasses and from 20 samples of porcine head meat. these results confirm that pigs are an important reservoir of pathogenic y. enterocolitica. however, contamination of carcasses during the slaughtering process wit ... | 1991 | 1854605 |
| expression cloning of yersinia enterocolitica o:3 rfb gene cluster in escherichia coli k12. | the genes of yersinia enterocolitica serotype o:3 (yeo3) that determine the synthesis of the o-side-chain of the lipopolysaccharide, the rfb region, were cloned into plasmid pbr322. the o-side-chain of yeo3 was expressed by the clone both in escherichia coli and salmonella typhimurium indicating that the entire rfb region was included in the clone. it was shown by restriction mapping, deletion analysis and transposition mutagenesis that about 10.4 kilobase pairs of dna was essential for the synt ... | 1991 | 1857198 |
| the effect of growth temperature on the biosynthesis of yersinia enterocolitica o:3 lipopolysaccharide: temperature regulates the transcription of the rfb but not of the rfa region. | the rfb region of yersinia enterocolitica o:3 (yeo3) that determines the synthesis of the o-side chain of the lipopolysaccharide was cloned and expressed in escherichia coli k12 previously. the clone did not show the in vitro temperature variation in o-side chain expression known for yeo3, instead analogous o-side-chain was produced at 25 degrees c and at 37 degrees c and both were similar to that produced by yeo3 at 25 degrees c. in northern blot analysis marked reduction in the amount of rfb-s ... | 1991 | 1857201 |
| the surface-located yopn protein is involved in calcium signal transduction in yersinia pseudotuberculosis. | the low-calcium response (lcr) is strongly conserved among the pathogenic yersinia species and is observed when the pathogen is grown at 37 degrees c in ca(2+)-depleted medium. this response is characterized by a general metabolic downshift and by a specific induction of virulence-plasmid-encoded yop genes. regulation of yop expression is exerted at transcriptional level by a temperature-regulated activator and by ca(2+)-regulated negative elements. the yopn gene was shown to encode a protein (f ... | 1991 | 1857212 |
| [the effect of yersinia pestis ev76 6 md plasmid on the composition of outer membrane proteins of yersinia pseudotuberculosis ypiii]. | on the basis of yersinia pseudotuberculosis strain ypiii the isogenic variants containing the different combinations of 47 md plasmids from yersinia pestis or yersinia pseudotuberculosis cells with the 6 md pyp plasmid from yersinia pestis ev (intact or having impaired the pla gene determining the synthesis of plasmocoagulase). the degradation of the secreted proteins encoded by the 47 md plasmids of yersinia pestis and yersinia pseudotuberculosis in the cells harbouring the 6md pyp plasmid has ... | 1991 | 1857369 |
| [detection of virulence markers in clinical strains of yersinia]. | the dispersion of plasmid pyv associated virulence markers in 474 yersinia strains isolated from people has been studied. the ability to autoagglutination, calcium dependence of growth and the specific antigens were identified in 157 strains of traditionally pathogenic yersinia enterocolitica serovars 03, 09, yersinia pseudotuberculosis serovar i. they were not found in 223 strains of other 12 serovars of yersinia enterocolitica, in 40 strains of yersinia frederiksenii, yersinia kristensenii, ye ... | 1991 | 1857370 |
| [the effect of a plague pathogen plasmid on lethality and immunogenicity]. | on the model of yersinia transconjugants (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) carrying the conjugative cointegrates containing the 47 and 65 md plasmids from yersinia pestis the data were obtained on the different affects of the latter plasmids on the lethality and immunogenicity conferred by the host bacterial cells. the plasmid effects were drastically different during bacterial infection in mice or guinea pigs. the possibility of appearance of the recombinan ... | 1991 | 1857372 |
| [the effect of the bacterial genome on the expression and behavior of the plasmid determining the ca2+-dependence of the plague pathogen]. | the conjugative cointegrate containing the 47 md plasmid of yersinia pestis has been transferred into the strains of the different yersinia (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) and escherichia coli ca. there appeared in the populations of recombinant yersinia under the conditions of ca2+ deficit at 37 degrees c the cells coming into the stasis stage or dying. it was shown on the model of yersinia enterocolitica that bacterial lethality might be prevented by exc ... | 1991 | 1857373 |
| [the heterogeneity of collection strains of yersinia pseudotuberculosis and their molecular biological properties]. | the capacity of y. pseudotuberculosis strains for disassociation with the appearance of s- and p-forms has been studied. strains 852 and 9547 show high stability in s-forms, their conversion into r-forms occurring at 40-42 degrees c. strain 6953 shows pronounced polymorphism and instability of its associations at different growth temperatures. strain 9532 exists in s- and r-forms which retain their stability during numerous subculturings at different growth temperatures and prolonged storage. th ... | 1991 | 1858469 |
| [the nonspecific changes in the immune system to the administration of the membrane-ribosomal fraction of the causative agent of pseudotuberculosis]. | nonspecific changes in different elements of the immune system of animals under the action of y. pseudotuberculosis membrane-ribosomal fraction (mrf) with high protective potency have been studied for the purpose of the analysis of the preparation for immunological safety. mrf has been shown to produce no changes in humoral immune response to antigens of different nature or to enhance this response, to produce no essential effect on the intensity of the reaction of delayed hypersensitivity to sh ... | 1991 | 1858471 |
| analysis of virc, an operon involved in the secretion of yop proteins by yersinia enterocolitica. | upon incubation at 37 degrees c in the absence of ca2+ ions, pathogenic yersiniae release large amounts of pyv plasmid-encoded proteins called yops that are involved in pathogenesis. yersinia enterocolitica also expresses an outer membrane protein that is considered an adhesin and called yada (previously called p1 or yopa). the production of yops is coordinately regulated by a 20-kb region of the plasmid referred to as the ca2+ dependence region and containing at least four loci called vira, vir ... | 1991 | 1860816 |
| [structure of the o-specific polysaccharide chain of the yersinia pseudotuberculosis lipopolysaccharide (serovar ii c)]. | an o-specific polysaccharide has been isolated on mild acid hydrolysis of lipopolysaccharide from yersinia pseudotuberculosis serovar iic and shown to consist of abequose, d-mannose and 2-acetamido-2-deoxy-d-galactose residues in the ratio 0.8:3:1. from the results of acid hydrolysis, 13c nmr, methylation and periodate oxidation studies the structure of the repeating unit of the o-specific polysaccharide is deduced as follows: (formula; see text) | 1991 | 1863285 |
| the microbiological quality of drinking water supplies of izmir city: the incidence of yersinia enterocolitica. | a total of 100 water samples collected from piped public supplies, wells with and without motor pumps, springs and commercially bottled spring waters were microbiologically examined. in order to determine microbiological quality, aerobic bacterial and coliform counts were estimated and the presence of escherichia coli and yersinia enterocolitica was investigated. of the samples tested, 85 of them met the specifications set by tfr (turkish food regulation) for coliforms, whereas 72 of them met th ... | 1991 | 1863530 |
| yersinia enterocolitica infection with ileal perforation associated with iron overload and deferoxamine therapy. | despite the frequency of gastrointestinal disease caused by yersinia enterocolitica, intestinal perforation is a rare complication of this infection and to date only eight cases have been reported in the english literature. we describe a case of this unusual condition in a 17-year-old male patient with thalassemia intermedia requiring transfusion, who was also taking deferoxamine. the severity of the clinical picture is probably due to the increased virulence of yersinia enterocolitica in the pr ... | 1991 | 1864209 |
| class-specific immune response to yersinia enterocolitica serotype o9 antigens as determined by enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay (elisa) using lipopolysaccharide (s-lps) as the antigen was used to analyze the antibody response in rabbits orogastrically and intravenously infected with virulent (plasmid-bearing) yersinia enterocolitica o9 strains (pyv+) and with the avirulent (plasmid-cured) derivatives (pyv-). a significative response of immunoglobulin g (igg), iga, and igm antibodies against the s-lps antigen was evident in sera from the rabbits orogastrically infected with pyv+ strain ... | 1991 | 1864943 |
| acute mesenteric lymphadenitis due to yersinia pseudotuberculosis lacking a virulence plasmid. | a serotype 4a strain of yersinia pseudotuberculosis lacking the virulence plasmid pyv (pyv- strain) was isolated from the mesenteric lymph nodes but not from the stool or the appendix of a 10-year-old girl with a diagnosis of acute mesenteric lymphadenitis. microscopically, reticulocytic abscess and lymphadenitis were persent in the enlarged mesenteric lymph nodes. antibody against the isolate was detected in the serum. the isolate was negative for the presence of plasmid pyv and plasmid pyv-med ... | 1991 | 1864948 |
| cationic yersinia antigen-induced chronic allergic arthritis in rats. a model for reactive arthritis in humans. | cationic antigens are known to have considerable arthritogenic potential in experimental systems. during a systematic search for suitable, naturally occurring candidates an intracellular protein was isolated from the ribosomal pellet of yersinia enterocolitica 0:3, a bacterial strain associated with reactive arthritis in humans. the protein is highly cationic, contains two 19-kd polypeptide chains linked by a disulfide bond, and reveals a strong tendency for spontaneous aggregation. it is sugges ... | 1991 | 1864972 |
| [immunoglobulin e level in acute yersinia infection]. | igg, ige, iga, igm levels were measured in patients with yersiniosis (generalized or mixed variant). acute period of the disease (week 1-2) was associated with elevated levels of ige in 59.1% of the patients which returned to normal on week 4-6. time course changes in iga, igg and igm concentrations were related to the synthesis of specific antibodies. ige level rise can be considered a defence mechanism contributing to elimination of yersinia antigens from the body. | 1991 | 1865657 |
| [variants in the manifestations of the generalized form of yersiniosis]. | the clinical manifestations of generalized yersiniosis were studied in 61 patients. primary generalized form was observed in 18 patients. predecessors were gastroenteritis, appendicular syndrome. clinical manifestations were polymorphous. all patients revealed fever and exanthema, 5%--catarrhal phenomena, 63.3%--lymphadenitis, 86%--arthralgia, 39.2%--gastrointestinal disorders, 114%--icteric syndrome. in 7.6% the course was protracted with recurrences of fever, exanthema, jaundice, diarrhea, for ... | 1991 | 1866933 |
| the presence of cis-9,10-methylene hexadecanoic acid in yersinia enterocolitica. | the fatty acids of yersinia enterocolitica were investigated by abbas and card. in their report, they stated that the major fatty acids were c16:0, c16:1, c17:0 and c18:1 and branched or cyclopropane side-chain fatty acids could not be detected. we, however, found a moderate amount of cyclopropane side-chain fatty acid. we could determine that this fatty acid was cis-9, 10-methylene hexadecanoic acid by gas-liquid chromatography, gas chromatography-mass spectrometry, silver-nitrate-treated tlc a ... | 1991 | 1870433 |
| [radioimmunological analysis in studying the persistence of the plague antigen in the body of rodents and ectoparasites in natural foci in siberia]. | to study the persistence of y. pestis capsular antigen, or fraction 1 (f1), in the body of less important plague carriers in the mountain altai and transbaikal natural foci, as well as in experimentally infected ticks, the liquid-phase competitive radioimmunoassay (ria) was used for the first time. in this study ria showed, due to its sensitivity, doubtless advantages over traditional methods, such as the passive hemagglutination (pha) test and the antibody neutralization (an) test, and made it ... | 1991 | 1872096 |
| [reactive arthritis caused by yersinia in children. report of 4 cases]. | among a group of children hospitalized for "joint pain", four of the cases of reactive arthritis were due to yersinia infection. these four cases are described. some patients had suggestive clinical symptoms but serologic studies were needed to establish the diagnosis. tissue group studies showed that two children were hla b27 and one was hla b7. reactive arthritis due to yersinia in a form of "enteral arthritis". the prevalence of yersinia arthritis has not been documented. serologic tests are ... | 1991 | 1872525 |
| an evaluation of three commercial fecal transport systems for the recovery of enteric pathogens. | twenty-five isolates, including six strains of shigella species, six strains of salmonella species, five strains of yersinia enterocolitica, six strains of campylobacter jejuni, and two strains of vibrio parahaemolyticus, were inoculated at a concentration of 1.5 x 10(4) colony-forming units/ml into the following transport systems: fekal enteric plus (trend scientific, inc., st. paul, mn), cary blair transport medium (remel, inc., lenexa, ks), and para-pak c & s (meridian diagnostics, inc., cinc ... | 1991 | 1877533 |
| multiple hepatic abscesses due to yersinia enterocolitica. | | 1991 | 1877943 |
| [the specificity of plague antigen fi in pha]. | it were inoculate a total of the 15 type and 13 group leptospira strains to the rattus flavipetus and rattus lose 137 rates in lei zhou peninsula. as the antisera acquired, it has been examined the lyso-agglutinative test in leptospira strains. up to standard serum it was to test for pha of plaque, employing the sensitized blood cell by fi from 4 antiplague institutes. all of tests are negative. in addition to test for 3 immune sera, hemorrhagic fever antisera (immunized animals apodemus agraius ... | 1991 | 1878960 |
| [cloning of the locus encoding synthesis of an outer membrane protein (omp2) of the calcium dependence plasmid of yersinia pestis (lehmann, neumann)]. | the genetic locus of yersinia pestis encoding synthesis of a 46-kda heat-inducible outer membrane protein (omp2) was cloned into pbr322 plasmid. the omp2 was shown to be analogous to previously described yoph and yop2b proteins. the fifth hindiii fragment of 48-mda calcium dependence plasmid pcad358 mediates production of 31- and 28-kda proteins, irrespective of orientation of the insertion. a 31-kda polypeptide seems to correspond to the yopj described elsewhere. the maps of bamhi and hindiii o ... | 1991 | 1879678 |
| distribution of the inva, -b, -c, and -d genes of salmonella typhimurium among other salmonella serovars: inva mutants of salmonella typhi are deficient for entry into mammalian cells. | invasion of intestinal epithelial cells is an essential virulence factor of salmonellae. a group of genes, invabc and invd, that allow salmonella typhimurium to penetrate cultured epithelial cells have previously been characterized (j. e. galán and r. curtiss iii, proc. natl. acad. sci. usa 86:6383-6387, 1989). the distribution of these genes among salmonella isolates belonging to 37 different species or serovars was investigated by southern and colony blot hybridization analyses. regions of hig ... | 1991 | 1879916 |
| health status of salmonids in river systems in natal. iii. isolation and identification of bacteria. | both pathogenic and non-pathogenic bacteria were isolated from fish, both salmonid and non-salmonid, from selected river systems in natal. pasteurella pisicida was isolated for the first time from fish in south africa. the isolation of yersinia ruckeri, aeromonas salmonicida, and edwardsiella tarda were recorded for the first time from fish in natal. a. hydrophila and flexibacter columnaris were found to be widespread throughout the river systems in natal. the streptococcus species which caused ... | 1991 | 1881658 |
| [cyclic nucleotides, prostaglandins and thrombocyte aggregation in rats poisoned with "murine" yersinia pestis toxin]. | y. pestis "mouse" toxin, introduced intraperitoneally into rats in a dose of ld100 [correction of lg100], produces phasic changes in the thrombin-induced aggregation of thrombocytes and the content of prostaglandins and cyclic nucleotides in them. as the result of the damaging action on the endothelium of blood vessels at the initial period of intoxication, the concentration of prostaglandin 6-keto f1 alpha in blood plasma and camp [correction of camr] in thrombocytes sharply decreases, which ca ... | 1991 | 1882634 |
| yersinia enterocolitica biotype 2 serotype o9 septicaemia in a previously fit man, raw goats' milk having been the apparent vehicle of infection: a cautionary tale. | a previously fit 66-year-old man presented with a 2 weeks' history of malaise, fever and vomiting which led to a septicaemic illness. yersinia enterocolitica biotype 2 serotype o9 was isolated from the patient's blood and from raw goats' milk remaining in a bottle after the patient had consumed some of the contents. he also produced antibodies to this serotype. careful history taking, however, revealed that the bottle of milk had been purchased after the patient became ill. milk from the same bo ... | 1991 | 1885916 |
| [uveitis after yersinia enterocolitica infection]. | yersiniosis is a food-born infection. it may cause an acute enterocolitis and trigger an anterior uveitis. we report a case of bilateral panuveitis with chorioretinitis - thus with the involvement of anterior and posterior uvea. because the agglutination titer against the yersinia enterocolitica type 3 was increased, we consider it as the most probable etiology of the panuveitis. a serological test for yersinia should be done in patients with uveitis, who consume food from various sources e.g. t ... | 1991 | 1886382 |
| nucleotide sequence of a new class a beta-lactamase gene from the chromosome of yersinia enterocolitica: implications for the evolution of class a beta-lactamases. | the nucleotide sequence has been determined of a 1400 bp fragment from the chromosome of yersinia enterocolitica containing the gene for beta-lactamase i. an orf of 882 bp was identified, which could code for a polypeptide of 294 amino acids, closely related to other beta-lactamases of molecular class a. amino acids 1-30 could constitute a signal peptide. the mature protein would be 264 amino acids long with a calculated pi of 6.2. alignment of the amino acid sequence of the class a beta-lactama ... | 1991 | 1886608 |
| selective isolation methods for fish pathogens. | | 1991 | 1887270 |
| [the results of the dynamic observation of patients with reactive yersinia arthritis]. | as many as 50 patients with reactive yersinia arthritis were followed up. there were 4 men and 46 women. the mean age of the patients was 31.1 yr. the mean follow-up period was 30.8 months. in 72% of the cases, the disease was preceded by diarrhea. during the first examination, mono-oligoarthritis was detected in 78% of the patients. extra-articular alterations (damage of muscles, ligaments, heart, skin changes, etc) were also recorded. the results of bacteriological analyses were positive in 22 ... | 1991 | 1887403 |
| [the rheumatic complications of pseudotuberculosis in a newly arrived population in siberia]. | | 1991 | 1887407 |
| [the comparative effectiveness of serological and microbiological studies in the etiological diagnosis of reactive arthritis]. | the information content of serological and microbiological studies was studied and compared in 72 patients suffering from enterogenous reactive arthritis. a high level of antibodies to intestinal yersinia was detected in 11.1% of the patients, positive coproculture in 8.3%. only one patient demonstrated at a time a high level of antibodies and isolation of intestinal yersinia coproculture. the causative agent could usually be isolated in patients with the symptoms of enterocolitis. it is assumed ... | 1991 | 1887408 |
| [pathology of the gastrointestinal tract in patients with reactive arthritis and its possible role in the pathogenesis of the disease]. | | 1991 | 1887410 |
| [the efficacy of bifidum-containing preparations in enterogenous reactive arthritis]. | analysis is made of the intestinal microflora in 43 patients suffering from reactive arthritides (rea) that developed after intestinal infection. the overwhelming majority of the patients manifested a decrease of the level of the bifidoflora. the disorders of the intestinal microflora were related to the disease activity and standing. bifidumbacterine was found to produce a significant beneficial effect on the course of rea and to cause no serious side effects. according to the preliminary data, ... | 1991 | 1887411 |
| [immune disorders and sensitization to microbial antigens in seronegative spondylitis]. | a study was made of immunity in seronegative spondylarthritis. in ankylosing spondylarthritis, reactive arthritides and psoriatic arthritis, the decrease of t cells, t helpers and t suppressors was identified. patients with ankylosing spondylarthritis and reactive arthritides manifested an increase of the b-lymphocyte count. patients with ankylosing spondylarthritis were demonstrated to be sensitive to klebsiella (52%), those with reactive arthritides to intestinal yersinia (44%). the phenotype ... | 1991 | 1887424 |
| isolation of yersinia enterocolitica in alexandria, egypt. | | 1991 | 1887497 |
| factors promoting acute and chronic diseases caused by yersiniae. | the experimental system constructed with the medically significant yersiniae provides a powerful basic model for comparative study of factors required for expression of acute versus chronic disease. the system exploits the close genetic similarity between yersinia pestis, the etiological agent of bubonic plague, and enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica. y. pestis possesses three plasmids, of which one, shared by the enteropathogenic species, mediates a number ... | 1991 | 1889045 |
| [sepsis caused by yersinia enterocolitica in a female patient with liver cirrhosis]. | as a primarily intestinal pathogen. yersinia enterocolitica (y. e.) may cause generalized infection in patients with malignant and other serious diseases or immunodeficient subjects. in certain conditions, elevated serum and tissue iron concentrations represent an additional risk factor for systemic infection with this opportunistic bacterium. in our patient, y. e. septicemia developed during liver cirrhosis decompensation. clinical signs of infection were alleviated by appropriate antibiotic th ... | 1991 | 1890909 |
| the low-ca2+ response virulence regulon of human-pathogenic yersiniae. | the lcr is thought to function when the yersiniae infect their mammalian host (see fig. 2). at present, all evidence points to roles of the lcr in protecting the bacteria in extracellular niches. we can speculate that early in infection, the yersiniae experience a 37 degrees c extracellular, ca(2+)-containing environment. temperature-induction and down-modulation by ca2+ would occur for lcr genes, resulting in a cytotoxic and weakly anti-phagocytic surface on the bacteria. yersiniae that are tak ... | 1991 | 1890954 |
| systemic yersinia enterocolitica infection associated with iron overload and deferoxamine therapy. | | 1991 | 1892033 |
| aspects of the epidemiology of yersinia enterocolitica: a review. | a review of works concerning different aspects of the epidemiology of human pathogenic yersinia enterocolitica biogroup iv/serogroup o:3 (y. enterocolitica o:3) is given. to investigate the epidemiology of y. enterocolitica o:3 in danish herds of pigs, tonsil swabs from 2218 freshly slaughtered pigs originating from 99 herds were examined. the organism was isolated from 25% of the pigs and from 82% of the herds. no herd management factor could be associated with the presence of the organism. the ... | 1991 | 1892741 |
| modelling the effect of ph, acidulant and temperature on the growth rate of yersinia enterocolitica. | growth of two pathogenic and one environmental serotype of yersinia enterocolitica under acidic conditions and at 4 and 25 degrees c was investigated. at both temperatures the maximum growth inhibitory ph depended on the acidulant used and was in the order acetic greater than lactic greater than citric greater than sulphuric. at the lower temperature the maximum growth inhibitory ph was 0.3-0.5 ph units higher than at 25 degrees c. no difference was observed between the behaviour of pathogenic a ... | 1991 | 1894580 |
| enterotoxigenic escherichia coli associated with infant diarrhoea in galicia, north-western spain. | to assess the role of enterotoxigenic escherichia coli (etec) in infantile diarrhoea, 482 children with diarrhoea and 103 healthy controls, from three localities of galicia, north-western spain, were investigated between 1985 and 1988. rotavirus (37.3%) and salmonella spp. (12.8%) were the most common causal agents, followed by etec (3.9%), campylobacter jejuni (2.3%), shigella spp. (0.9%) and yersinia enterocolitica (0.5%). etec were significantly more frequently isolated from children with dia ... | 1991 | 1895327 |
| identification and cloning of a fur regulatory gene in yersinia pestis. | yersinia pestis is one of many microorganisms responding to environmental iron concentrations by regulating the synthesis of proteins and an iron transport system(s). in a number of bacteria, expression of iron uptake systems and other virulence determinants is controlled by the fur regulatory protein. dna hybridization analysis revealed that both pigmented and nonpigmented cells of y. pestis possess a dna locus homologous to the escherichia coli fur gene. introduction of a fur-regulated beta-ga ... | 1991 | 1898928 |
| travelers' diarrhea among united states military personnel during joint american-egyptian armed forces exercises in cairo, egypt. | a study was conducted of travelers' diarrhea in a united states military population on deployment in cairo, egypt, during july and august 1987. acute diarrhea requiring medical attention developed in 183 (4%) of 4,500 troops. a possible etiologic agent was identified in 49% of all diarrhea cases. enteric pathogens associated with cases of diarrhea included: enterotoxigenic escherichia coli (17% st-producers, 13% lt-producers, and 3% lt/st-producers); shigella (9%); campylobacter spp. (2%); salmo ... | 1991 | 1900113 |
| update: yersinia enterocolitica bacteremia and endotoxin shock associated with red blood cell transfusions--united states, 1991. | from april 1987 through august 1988, four cases of yersinia enterocolitica bacteremia associated with red blood cell (rbc) transfusions were reported to cdc. this report summarizes findings from the investigation of an additional six cases reported from six different states from january 1989 through february 1991. | 1991 | 1900562 |