| pharmacokinetics of trimethoprim-sulfamethoxazole in children. | the present report extends experience with the use of trimethoprim-sulfamethoxazole (tmp-smx) in children aged 3 months to 10 years. the regimen was tmp (200 mg)--smx (1000 mg)/m-2d given in two equal doses. the drug was easily administered, well tolerated and efficacious in the treatment of a variety of infections in 12 children. a steady state had been achieved by the third dose of medication and accumulation of either component during days 1 through 4 did not occur. serum concentrations of tm ... | 1975 | 1093656 |
| studies of the mechanisms of radiosensitization of bacterial and mammalian cells by diamide. | diamide sensitizes bacterial and mammalian cells to radiation by at least two mechanisms. sensitization of v79-gl1 chinese hamster cells is due mainly to a reduction of a reduction of the survival-curve shoulder, is observed both in oxygen and in hypoxia,and is additive to the sensitization of hypoxic cells by some nitroimidazoles. in contrast, sensitization of the radioresistant organism, micrococcus sodonensis, which has apronounced shoulder, is entirely dose-modifying. in a rapid-mix study us ... | 1975 | 1093986 |
| reversible inactivation of the isocitrate dehydrogenase of escherichia coli ml308 during growth on acetate. | during aerobic growth of escherichia coli ml308 on acetate as sole carbon source, the apparent synthesis of isocitrate dehydrogenase was repressed relative to cultures on other carbon sources, such as glucose, which do not employ the glyoxylate bypass as an anaplerotic sequence. when cells were removed from an acetate medium, or when compounds were added which made the operation of the glyoxylate bypass unnecessary, the activity of isocitrate dehydrogenase rapidly increased 3- to 4-fold but fell ... | 1975 | 1094097 |
| cinoxacin: in vitro antibacterial studies of a new synthetic organic acid. | cinoxacin (compound 64716) is a synthetic organic acid with antibacterial activity against most aerobic gram-negative bacilli. minimal inhibitory concentrations of cinoxacin (agar-dilution method) were determined for 419 strains. escherichia coli was the most susceptible group of organisms. the majority of klebsiella sp., enterobacter sp., proteus sp., and serratia marcescens were inhibited by 8 mug of cinoxacin per ml. pseudomonas aeruginosa and all gram-positive isolates tested were resistant ... | 1975 | 1094949 |
| antibiogram and lipid analysis of a pigmented strain of serratia marcescens and its nonpigmented variants. | antibiograms and lipid analyses of serratia marcescens pigmented strain 08 and its nonpigmented variants are compared. the overall lack of significant differences between pigmented and nonpigmented strains suggests that the role of pigment formation may not be related to antibiotic susceptibility. | 1975 | 1094951 |
| strike-through of moist contamination by woven and nonwoven surgical materials. | a test is described which correlates the stress of stretching surgical gown and drape material with moist bacterial strike-through. by application of this test to a number of woven and nonwoven surgical gown and drape materials, it was found that not all of these materials, either woven or nonwoven, are impermeable to moist contamination for equal periods of time. nonwoven disposable materials now in use range from those which remain impermeable to moist bacterial permeation through all tests wh ... | 1975 | 1094972 |
| evacuated blood-collection tubes--the backflow hazard. | five cases of nosocomial infection caused by serratia marcescens were traced to backflow of blood from nonsterile evacuated blood-collection tubes. the mechanism of backflow was investigated theoretically and the conditions were determined under which backflow can occur. the theory was confirmed by experiments conducted on a simulated venous system and by measurements of the venous pressure in the brachial vein of a patient during application and removal of a tourniquet. various possible solutio ... | 1975 | 1095169 |
| prodigiosin-like pigments. | prodigiosin, the bright red tripyrrole pigment from serratia marcescens, has also been identified in pseudomonas magnesiorubra, vibrio psychroerythrus, and two gram-negative rod-shaped mesophilic marine bacteria not members of the genus serratia. prodigiosin is sometimes bound to proteins; thus, extracts may require acid treatment before isolation of the pigment. higher homologs of prodigiosin have been detected by mass spectroscopy. a mutant strain of s. marcescens produced nor-prodigiosin, in ... | 1975 | 1095305 |
| relation of structure to function in bacterial o-antigens--vii. endotoxicity of 'lipid a'. | | 1975 | 1095471 |
| testing the mutagenic potency of chemical substances in a linear host-mediated assay. i. experimental microbiological basis. | by the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. the microbiological basis for a linear-method is presented in which the population growth of the auxotrophic and substance-induced mutants used int he test can be described mathematically during their logarithmic growth phase with regression lines. | 1975 | 1095922 |
| [letter: risk of contamination in vacuum blood sampling systems]. | | 1975 | 1095945 |
| serratia bacteremia from mean arterial pressure monitors. | | 1975 | 1096683 |
| antibiotic resistance patterns of clinical isolates of serratia marcescens. | antimicrobial susceptibility patterns of 102 clinical isolates of serratia marcescens from three medical centers were studied by using disk sensitivity and agar dilution methods. the least resistance was demonstrated against gentamicin, nalidixic acid, chloramphenicol, and sulfisoxazole, all of which inhibited more than 80% of the strains. cephalothin was completely ineffective, and more than 90% of strains were resistant to ampicillin and tetracycline. as demonstrated by the agar dilution metho ... | 1975 | 1096799 |
| plasmodium berghei: immunologic enhancement of antigen by adjuvant addition. | | 1975 | 1097263 |
| infrared scattering: a method for evaluating the mass and size of bacteria. | in the realm of biophysics and biochemistry, light scattering is used extensively to evaluate the molecular weight and size of macromolecules and particles in suspension. bacteria scatter strongly but are too large for the conventional procedures. by extending the wavelength lambda of the incident radiation into the infrared, we show that the effective size of the bacteria (relative to lambda) is reduced, and the usual zimm plot measurements and procedure can be applied to evaluate the molecular ... | 1975 | 1098063 |
| refractive index increment meaasurement for bacterial suspensions. | | 1975 | 1098064 |
| functional role of antibody against "core" glycolipid of enterobacteriaceae. | antibodies against the "core" glycolipid of enterobacteriaceae (2-keto, 3-deoxyoctonate-lipid a) have been associated with protection against the sequelae of gram-negative rod bacteremia. to investigate the nature of this protection, dogs and rabbits were immunized with purified glycolipid prepared by phenol-chloroform-petroleum ether extraction of the "re" mutant of salmonella minnesota 595 and opsonophagocytic and bactericidal tests were carried out using lapine peritoneal granulocytes and ser ... | 1975 | 1099119 |
| arginine gene cluster of serratia marcescens. | biochemical and genetic studies on the arginine-requiring auxotrophs derived from a serratia marcescens strain were carried out. the arg mutants were classified into seven biochemical groups based on their growth response to five precursors of arginine biosynthesis and enzyme deficiency. reciprocal transduction tests among these arg mutants divided them into three linkage groups, and the fine mapping in each of the groups by two- or three-point crosses revealed the following arrangement of loci. ... | 1975 | 1099287 |
| activity of minocycline against acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and serratia marcescens. | the activity of minocycline and tetracycline against 23 isolates of acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and 178 strains of serratia marcescens was determined by disk and microdilution methods. the results indicate that minocycline is highly active against this species of acinetobacter, all but one strain being inhibited by 0.007 mug of the antibiotic per ml. tetracycline was also active, though to a lesser degree, against a. calcoaceticus. of the 178 strains of ... | 1975 | 1099982 |
| [the influence of the antivitamine pyrithiamine on the metabolism of the thiamin-autotrophic serratia marcescens]. | a new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. the method uses a mutant of lactobacillus fermenti (atcc 9338) stimulated by pyrithiamine. by this specific method it is found that serratia marcescens is able to consume and to phosphorylate the antivitamine (presumably to pyrithiamine monophosphate). the uptake of the analog influences neither the generation time of serratia marcescens nor the biomass of t ... | 1975 | 1100010 |
| studies on polysaccharides from serratia marcescens. ii. on the structures of serratigen and serratimannan. | | 1975 | 1100283 |
| effects on specific antibodies on the catalytic activity of l-asparaginase from serratia marcescens and escherichia coli. | rabbit antisera against highly purified l-asparaginase from serratia marcescens and from escherichia coli showed up to 60% inhibition of the catalytic amidohydrolysis of l-asparagine when combined with the homologous enzyme. this inhibition was diminished somewhat against the heterologous enzyme. kinetic studies in the presence of these antisera showed an increased kmapp for both homologous and heterologous enzymes using l-asparagine as substrate. in contrast, kinetic studies employing the poor ... | 1975 | 1100609 |
| growth potential of cottonseed culture media for various clinically significant aerobic bacteria. | enzymatic hydrolysates of various cottonseed flours were prepared with the proteolytic enzymes bromelain, ht-200, pronase, and trypsin. the growth of various aerobic bacteria of clinical significance in these hydrolysates was compared to that obtained with a standard casein-soybean peptone culture medium, trypticase soy. the generation times of the majority of bacteria grown in the bromelain cottonseed flour hydrolysate were shorter than that obtained with the standard control broth. a bromelain ... | 1975 | 1100668 |
| interferon production in tortoise peritoneal cells. | | 1975 | 1100778 |
| amoxycillin and ampicillin. a comparative study of in vitro sensitivity and induced morphological alterations in serratia marcescens. | in vitro antibacterial activities of ampicillin and amoxycillin were compared against pigmented and non-pigmented strains of serratia marcescens. ampicillin appeared more effective than amoxycillin; three-fourths of all strains consistently exhibited an ampicillin minimum inhibitory concentration (mic) of at least one tube less than that recorded for amoxycillin. complete cross resistance was not observed as has previously been inferred. further, greater bactericidal activity was demonstrated wi ... | 1975 | 1100890 |
| reduction of airborne microorganisms by filtering recycled air in a chick hatcher. | an experimental chick hatcher designed to filter recycled ventilation air was tested for its effectiveness in reducing the number of viable airborne microorganisms. chicks in a filtered hatcher and a control hatcher (no filter) were artificially contaminated with serratia marcescens as ventilation air was recycled in the hatchers for twelve hours. the number of viable s. marcescens particles in the filtered air of the conditioning chamber was less than detectable. the number of viable airborne s ... | 1975 | 1101244 |
| the role of the nurse epidemiologist in infection control and continuing education. | a university affiliated veterans administration hospital had an increase in the number of serratia isolates. an epidemiologic investigation, including personnel and environmental prevalence study, helped correct errors in technique, management and judgment. a nurse epidemiologist prevented a cluster of infections from becoming an epidemic. | 1975 | 1101420 |
| relation of beta-lactamase activity to antimicrobial susceptibility in serratia marcescens. | one-hundred clinical isolates of serratia marcescens were tested for susceptibility to cephalothin, carbenicillin, ticarcillin, ampicillin, and cefoxitin. the majority of the 100 isolates (>/=70%) were susceptible to carbenicillin, ticarcillin, and cefoxitin; less than one-half were susceptible to ampicillin; none were susceptible to cephalothin. ten isolates from the 100 organisms tested were selectively assayed for their beta-lactamase activity. enzyme activity was measured using either iodome ... | 1975 | 1101819 |
| effect of surfactants on antibiotic resistance. | the effectiveness of surfactants as potentiators of antibiotic activity on several resistant strains of bacteria, selected from clinical sources and laboratory collections, was studied using a tube dilution assay. bacterial strains included members of the enterobacteriaceae and staphylococci. cetyltrimethylammonium bromide (ctab), tween 80 (tw80), a mixture of n-alkyldimethyl betaines (l14), and alpha-(2,4,5-trichlorophenoxy) propionic acid (tcp) were tested in combination with pencillin g (peng ... | 1975 | 1101823 |
| potent combinations of beta-lactam antibiotics using the beta-lactamase inhibition principle. | several penicillins known to be stable to enterobacterial beta-lactamases were tested in combination with beta-lactamase-sensitive penicillins and cephalosporins in a turbidimetric system. nafcillin was found to be the best beta-lactamase inhibitor amongst agents presently available commercially, but the related, new semi-synthetic penicillin brl 1437 (2-isopropoxy-1-naphthylpenicillin) was consistently found to be superior. using 103 ampicillin-resistant coliform bacilli and antibiotic levels a ... | 1975 | 1102266 |
| [contaminated infusions as cause of nosocomial serratia marcescens septicaemia in children (author's transl)]. | at the university children's clinic at munich 25 cases of serratia marescens septicaemia (mainly bacteriocin types 18 and 44) occurred within one year, predominantly in newborns and infants. almost all of the children were seriously ill from an underlying illness or had been operated on. two thirds had received antibiotics before the septicaemia occurred but they were ineffective. of a total of nine antibiotics tested against 51 serratia marcescens strains, nalidixic acid (99% sensitivity) and a ... | 1975 | 1102282 |
| antitumor activity of polysaccharides from serratia marcescens. | a lipopolysaccharide (serratigen) and a polysaccharide (serratimannan) were isolated from serratia marcescens, red strain no. 51. they were different from any other polysaccharides previously reported. the antitumor activity of these polysaccharides was determined. serratimannan showed 63% tumor inhibition and serratigen 38%, at a dose of 150 mg/kg, against solid tumor of sarcoma-180 using icr mice. the fraction obtained by removal of proteins from the crude extract by the sevag method showed a ... | 1975 | 1102378 |
| [antibody detection in infections with serratia marcescens (author's transl)]. | a report is given on the detection by agglutination and precipitation of antibodies against homologous strains in infections with serratia marcescens. the results of the investigation indicate that antibody formation is likely if invasive forms are present. the agglutination titers were between 1:40 and 1:640. it can be presumed that, in addition to the patient's antibodies against the o antigen, antibodies are also formed against certain antigens with the character of ferments or toxins. | 1975 | 1102453 |
| enumeration of high numbers of bacteria using hydrophobic grid-membrane filters. | printing a wax grid on a conventional membrane filter yields a device functioning as a most probable number apparatus (mpn), used at a single dilution but with a very large number of growth compartments (e.g., 3,650). by restraining the lateral spread and confluence of colonies, the hydrophobic grid-membrane filter (hgmf) allows growth- or colony-forming units (gu) to be resolved at levels far above those which produce an uncountable lawn on a conventional membrane filter. it also eliminates the ... | 1975 | 1103728 |
| serratia arthritis in heroin users. | septic arthritis due to serratia species was seen in four users of intravenous heroin. in all cases, the organism was cultured from joint aspirates. both the clinical presentation and the involvement of the sacro-iliac and knee joints were notably similar to the pseudomonas septic arthritis encountered in other heroin users. all four patients responded satisfactorily to therapy although one had a residual flexion contracture of the knee. twelve previously reported cases of serratia arthritis are ... | 1975 | 1104628 |
| mediation systems in bacterial lipopolysaccharide-induced hypotension and disseminated intravascular coagulation. i. the role of complement. | we have studied the role of complement in lipopolysaccharide (lps)-induced hypotension and disseminated intravascular coagulation (dic) by comparing the effects of injection of three preparations of lps from e. coli 0111:b4, s. minnesota re595, and s. marcescens. injections of nonlethal doses of these lps preparations into normal rabbits produced decreases in mean arterial blood pressure during a 5-h period. when rabbits treated with cobra venom factor (cof) to deplete c3 were injected with the ... | 1975 | 1104750 |
| first reported successful management of serratia marcescens bacteremia after open heart surgery in a child. | a 7 and one-half yr-old girl developed bacteremia from s. marcescens following debanding of the pulmonary artery and closure of multiple ventricular septal defects with a dacron patch and multiple teflon pledgets. the site of entry was probably a radial arterial catheter left in place for 8 days. infection was eradicated by a combination of gentamicin and carbenicillin over a 4-wk period. of 12 cases of postoperative serratia bacteremia in adults following valve replacement, only four survived. ... | 1975 | 1104800 |
| letter: serratia vertebral osteomyelitis in narcotic addicts. | | 1975 | 1106280 |
| serratia marcescens endocarditis: a regional illness associated with intravenous drug abuse. | from 1969 to 1974, 19 cases of serratia marcescens endocarditis were observed in the san francisco bay area. seventeen patients were intravenous drug users, and serratia caused 14% of all addict-associated endocarditis in san francisco. serratia strains were nonpigmented and had typical antibiotic sensitivities, except that 9 of the isolates exhibited colonial variation, with each variant having different antibiotic sensitivities. aortic or mitral valves were involved in 13 patients, and heart f ... | 1976 | 1106290 |
| physical properties of l-asparaginase from serratia marcescens. | purified l-asparaginase from serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. a subunit molecular weight of 31,500 +/- 1,500 was estimated for the enzyme after treatment with sodium dodecyl sulfate and urea and electrophoresis on polyacrylamide gels; a similar value was obtained by high-speed sedimentation equilibrium in ... | 1976 | 1107330 |
| host resistance to serratia marcescens infection: serum bactericidal activity and phagocytosis by normal blood leukocytes. | serratia marcescens strains isolated from clinical specimens can be divided into those which are sensitive or resistant to the bactericidal activity of normal human serum. serum bactericidal activity is heat labile, cation dependent, and is absorbable by whole, serum-sensitive serratia or ethanol-insoluble extracts of these organisms. bacteremic serratia infection is invariably caused by the serum-resistant strains. serum resistant serratia are ingested and killed by normal human leukocytes and ... | 1976 | 1107450 |
| ascending cholangitis diagnosed by percutaneous hepatic aspiration. | | 1976 | 1107502 |
| carbenicillin in acute renal failure. | three septicaemic patients with acute renal failure required carbenicillin. septicaemia was caused by pseudomonas in 2 patients and by serratia marcescens in the third. therapy in the first 2 patients was complicated by massive gastro-intestinal and uterine bleeding. septicaemia in the third patient was initially uncontrolled owing to inadequate serum levels of carbenicillin, despite increased dosage as renal function improved. the problems and indications for the use of carbenicillin in renal f ... | 1975 | 1108235 |
| biochemical and serological characteristics of serratia marcescens isolated from various clinical and environmental sources. | | 1975 | 1108841 |
| tryptophan biosynthetic pathway in the enterobacteriaceae: some physical properties of the enzymes. | several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. five different patterns were noted. differences in the anthranilate synthetase (as) and phosphoribosylanthranilate transferase (prt) defined these patterns. in all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and g-200 and thus probably ... | 1975 | 1116988 |
| activity of three aminoglycosides and two penicillins against four species of gram-negative bacilli. | three aminoglycoside antibiotics and two penicillins were compared for their in vitro activity against 60 isolates of serratia, pseudomonas, proteus mirabilis, and indole-positive proteus sp. testing was done by the agar dilution method using mueller-hinton broth solidified with 1.5% agar. the activity of amikacin, aminodeoxybutirosin, and gentamicin against proteus and pseudomonas, as related to their peak blood levels, showed no significant differences. amikacin was the most active against ser ... | 1975 | 1137369 |
| effects of nitrogen dioxide and 3-methylcholanthrene on pulmonary enzymes. | guinea pig lung phenol-o-methyltransferase, catechol-o-methyltransferase, and benzpyrene hydroxylase activities were examined after nitrogen dioxide and 3-methylcholanthrene treatment. while benzypyrene hydroxylase activity was enhanced by 3-methylcholanthrene, none of the pulmonary enzyme activities was altered after exposure to either 40 or 70 ppm of nitrogen dioxide for 2 hr. | 1975 | 1151721 |
| proceedings: the effect of tris on the response of various isolated, mammalian preparations to nerve and drug stimulation. | | 1975 | 1151799 |
| proceedings: effects of histamine, 2-methylhistamine and 4-methylhistamine on blood pressure and vascular resistance in the cat. | | 1975 | 1151865 |
| [a case of parieto-pulmonary acquired shunt presenting with hemoptysis (author's transl)]. | the authors report the case of a 52 year old man with arteriovenous shunt developing from the 7th left intercostal artery, revealed by hemoptysis and proved by intercostal selective arteriography. the patient had a past history of serofibrinous pleurity 29 years previously and had undergone paracentesis at this level. the origin of this shunt is then discussed. | 1975 | 1151917 |
| [effect of an oxygen-vitamin complex on general and immunological reactivity of irradiated animals]. | | 1975 | 1153721 |
| a simple and economical method for marking the egg r-wave on film in cinefluorography. | a new method for marking the r-wave of the egg on ciné film was developed. the system utilizes the absence of one control pulse from the camera immediately after r-wave. the missing pulse causes one unexposed frame on the film, which in turn acts as a marker. the equipment costs about 500 fmk. (similar to $132.00). | 1975 | 1153749 |
| protein-energy interrelationships during dietary restriction: effects on tissue nitrogen and protein turnover. | young adult female rats were fed diets containing either 50 or 0% lactalbumin at levels of 2, 4 or 6 g diet/day for 2, 8, and 16 days. there was no other protein in the diet. tissue nitrogen and loss of radioactivity from tissues labeled with 14c- and 3h-glutamate were measured. in a second study, similar rats were fed graded levels of lactalbumin at food intake levels of 3, 5, or 7 g/day. change in tissue nitrogen varied with the tissue, the time of observation, and the severity of the food res ... | 1975 | 1165856 |
| structural similarity of the membrane envelopes of rhizobial bacteroids and the host plasma membrane as revealed by freeze-fracturing. | the freeze-fracture technique was used to study the host plasma membrane and the membrane envelope of bacteroids in rhizobial root nodules of three host-rhizobium combinations. in all three combinations studied, the membrane envelopes of bacteroids are structurally similar to their host plasma membrane. however, the membrane appears to be reversed, because the number and arrangement of particles in the outer fractured face (face a, concave) and in the inner fractured face (face b, convex) of the ... | 1975 | 1168633 |
| agarose beads as matrices for proteins in cytophotometric investigations of immunohistoperoxidase procedures;. | quantitative aspects of direct immunohistoperoxidase procedures were studied in a model system consisting of agarose beads to which antigens or antibodies had been coupled. it could be proven that the final amount of reaction product resulting from the histoperoxidase reaction with 3,3-diaminobenzidine-tetra hcl in a bead was linearly related to the volume of the beads and to the staining time. this implies that protein-coupled agarose beads are a suitable model for the study of stoichiometric a ... | 1975 | 1168666 |
| binding of antigen by immunocytes. ii. effect of specific ig on antigen binding by mopc 315 cells. | the effect of specific immunoglobulin (ig) on specific binding of antigen to cells has been studied in a model system consisting of nurine myeloma cells (mopc 315), mopc 315 serum, and dnp conjugates. mopc 315 serum, which has iga specific for dnp, specifically inhibited the binding of dnp conjugates to these cells. using this model it was found that cells have a marked advantage over free specific ig in binding multivalent antigen molecules and retaining them in a bound state. cells were able t ... | 1975 | 1168694 |
| derivative absorption spectrophotometry of native proteins. | | 1975 | 1169006 |
| interference by manganous, cobaltous, and mercuric chlorides with the lowry protein determination. | | 1975 | 1169009 |
| incorporation of n-acetylglucosamine into the slime sheath of the cellular slime mould dictyostelium discoideum. | | 1975 | 1169163 |
| specificity of antisera raised by steroid ring-b oxime--bovine serum albumin conjugates. | | 1975 | 1169174 |
| transcellular ion route in rabbit gallbladder. electric properties of the epithelial cells. | the intracellular potential in gallbladder epithelial cellsis about minus 59 mv with respects to both mucosal and serosal media. it is a diffusion potential mainly due to k+;na+ conductance seems to be very low. entrance of cl- into cellsappears to be coupled with na+ a neutral carrier and exit towards blood side seems to be due to a nacl neutral pump. | 1975 | 1171431 |
| use of bile-esculin agar for rapid differentiation of enterobacteriaceae. | bile-esculin agar has been used for several years for the presumptive identification of group d streptococci. all members of the enterobacteriaceae family will also grow on this medium, but only certain ones can hydrolyze esculin to 6,7-dihydroxycoumarin, which reacts with iron to produce a characteristic blackening of the medium. one thousand and six cultures from clinical specimens representing 20 genera were isolated and identified. heavy inocula from fresh pure culture isolates on heart infu ... | 1975 | 1176613 |
| demonstration of amitriptylene in blood levels following a single oral dose in human. | | 1975 | 1178677 |
| prostaglandin e1 and f2alpha specific binding in bovine corpora lutea: comparison with luteolytic effects. | preliminary characterization indicated the presence of separate prostaglandin (pg)e1 and (pg)f2alpha binding sites in membrane fractions prepared from bovine corpora lutea. these differ in the rate and temperature dependence of the specific binding. equilibrium binding data indicate the apparent dissociation constants as 1.32 x 10(-9)m and 1.1 x 10(-8)m for pge1 and pgf2alpha, respectively. competition of several natural prostaglandins for the pge1 and pgf2alpha bovine luteal specific binding si ... | 1975 | 1178910 |
| new mechanisms for the biosynthesis and metabolism of 2-keto-l-gulonic acid in bacteria. | l-sorbose is oxidized to 2-keto-l-gulonic acid (kga) via the following sequence of reactions which we call the "sorbosone pathway": l-sorbose in equilibrium l-sorbosone leads to kga. the first step is reversible and is mediated by enzymes found in a soluble fraction obtained from pseudomonas putida atcc 21812. although no cofactor requirements were found for the forward reaction, the reverse reaction clearly required nadh. enzymes for this nadh-dependent synthesis of l-sorbose could be different ... | 1975 | 1182275 |
| estradiol-17beta biosynthesis in cultured granulosa cells from hypophysectomized immature rats; stimulation by follicle-stimulating hormone. | granulosa cells isolated from the ovaries of hypophysectomized immature rats synthesize and secrete estradiol-17beta and estrone when grown for 2 days in monolayer culture in a synthetic medium containing testosterone (0.5 mum) and a highly purified follicle-stimulating hormone (fsh) preparation (0.25 mug/ml). secretion is negligible in the absence of either testosterone or fsh, and a highly purified luteinizing hormone (lh) preparation (0.25 mug/ml) was without significant stimulatory effect. i ... | 1975 | 1183412 |
| cell wall composition of novobiocin-resistant pleiotropic mutant staphylococci. | physically purified cell walls were prepared from selected pleiotropic novobiocin-resistant staphylococcal strains. the quantitative amino acid, amino sugar, and phosphorus contents of these walls are reported. this pleiotype was culturally diagnosed by its inability to support the growth of typing phages, inability to release latent bacteriophage, failure to elaborate coagulase, altered sugar catabolic pattern, and resistance to novobiocin. the strains were divided into two groups on the basis ... | 1975 | 1184576 |
| a vector model of disease for teaching and diagnosis. | | 1975 | 1196157 |
| effect on microorganisms of volatile compounds released from germinating seeds. | volatile compounds evolved from germinating seeds of slash pine, bean, cabbage, corn, cucumber, and pea were evaluated for their ability to support growth of microorganisms in liquid mineral salts media lacking a carbon source. growth of eight bacteria was measured turbidimetrically and of six fungi as dry weight of mycelium. volatiles caused increased growth of pseudomonas fluorescens, bacillus cereus, erwinia carotovora, agrobacterium tumefaciens, a. radiobacter, rhizobium japonicum, mucor muc ... | 1975 | 1201509 |
| effect of level of feeding during lactation on sow and pig performance. | | 1975 | 1206012 |
| [ability of yeast from the genus candida to assimilate sugars and acetic acid from mineral medium and sulfite liquors]. | | 1975 | 1214648 |
| [determination of human placental lactogen in the blood of pregnant women by rcc-hpl-ria kit--experimental studies]. | | 1975 | 1240044 |
| [concentration of antibody to arboviruses in mouse immune ascitic fluid]. | a method for increasing titers of antibody in mouse immune ascitic fluids by twostep concentration of defibrinized ascitic fluids by means of the cryomethod and lyophilization has been developed. antibody titers increased 8-32-fold. | 1975 | 1240698 |
| induction of a coupling defect in rats during inhibition of tyrosine dehalogenase. | we have reported earlier that administration of 3-nitro-l-tyrosine (mnt), 8 mm in drinking water, to rats receiving a low iodine diet (lid) results in greater tsh secretion, larger goiters, and more rapid uptake and release of radioiodine than lid alone, and ultimately may produce hypothyroidism. these findings have been confirmed, and hypothyroidism documented by demonstrating depressed levels of hepatic mitochondrial alpha-glycerophosphate dehydrogenase. also, prolonged treatment with mnt + li ... | 1976 | 1248438 |
| renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system. | reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ... | 1976 | 1248479 |
| [editorial: helsinki declaration.ii]. | | 1976 | 1251534 |
| "hybrid" myosin filaments from smooth and striated muscle. | | 1976 | 1255715 |
| epidemiologic aspects of nosocomial infections. | in a currently ongoing surveillance of hospital-associated infections in two metropolitan hospitals in oklahoma, the results over a four-month period suggest at least tentative principles of host-agent relationships in hospital-associated infections. within the urinary tract, the site with the highest number of total isolations, escherichia coli and klebsiella pneumoniae were the most common pathogens. by age, the under 5 and over 60 year age groups had the highest number of isolations. staphylo ... | 1976 | 1257828 |
| dna relatedness among species of enterobacter and serratia. | species of enterobacter and serratia were examined for deoxyribonucleic acid relatedness to klebsielleae, to atypical erwiniae, and to other members of enterobacteriaceae. deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. strains of enterobacter cloacae formed two separate hybridization groups that correlate with the presence or absence of yellow pigment. pigmented e. cloacae were 75-100% related, but they were only 40-50% re ... | 1976 | 1260520 |
| editorial: brucellosis test developments. | | 1976 | 1265976 |
| kennel cough: vaccination against bordetella bronchiseptica infection. | | 1976 | 1266000 |
| teratologic evaluation of dermally applied zinc pyrithione on swine. | | 1976 | 1273846 |
| radioimmunoassays for free and conjugated trienbolone and for trienbolone acetate in bovine tissue and plasma samples. | a specific, sensitive, precise and accurate radioimmunoassay has been developed for the quantitation of the synthetic anabolic steroid trienbolone acetate (tba) and its major metabolites, free and conjugated trienbolone (tboh) in bovine tissues and plasma. with the extraction procedure described unspecific interference with the antigen-antibody reaction could be ruled out. the assay can significantly detect amounts of more than 40 pg tboh and 70 pg tba. 0.1 - 2.0 g tissue and 0.1 - 1 ml plasma a ... | 1976 | 1273899 |
| [formation of bactericidal substances by cosmarium impressulum (author's transl)]. | 1. an already published test method for detecting bactericidal substances in paper chromatograms was further improved. 2. in cultures of cosmarium impressulum free from bacteria, two bactericidal substances were found in the ether extracts from the algae and two others in the extracts of the culture medium. the are active against some or all bacteria testes (serratia marcescens, pseudomonas fluorescens; aerobacter aerogenes or bacillus pumilus). 3. if the culture medium of cosmarium or another d ... | 1976 | 1275650 |
| [antibody avidity in candidiasis patients]. | the properties of antibodies to candida albicans in healthy persons, candidiasis patients and patients with somatic pathology of nonmycotic genesis were studied. healthy persons were found to have the highest avidity of antibodies at the minimal neutralizing dose of the antigen. in all groups the titers of antibodies were inversely related to their avidity. | 1992 | 1279913 |
| [ammoniacal silver method in alkaline ph range as a technique for detection of morpho-functional features of nerve tissue elements]. | an alkaline range ph 9.1-12.3 instead of values ph 7.0-7.1 was used to localize lysine-rich and arginine-rich nucleoproteins by ammonia silver (as) staining method (black and ansley, 1966) in brain structures of rabbits and rats. this modification of as method made it steady to reveal lysine-rich and arginine-rich nucleoproteins and was able to demonstrate the high variability of protein localization in relation to the balance of essential amino acids in the brain structures with different funct ... | 1992 | 1280174 |
| expression of intermediate filaments and actins in human dental pulp and embryonic dental papilla. | the localization of different cytoskeletal proteins (keratin, vimentin, desmin, actin, and alpha-smooth muscle actin) was examined by immunohistochemistry in normal human adult dental pulp and compared with dental papilla of tooth germs. keratin and actin were localized in enamel organ. vimentin and actin were observed in the dental papilla and in the adult dental pulp. desmin and alpha-smooth muscle actin were present only in the vessel walls. these data are discussed paying particular attentio ... | 1992 | 1280923 |
| use of a biological extract of serratia marcescens to decrease doxorubicin-induced myelosuppression in dogs. | fifteen dogs were given doxorubicin, iv, at a dosage of 30 mg/m2 of body surface. a commercially available biological extract of serratia marcescens (besm) was administered sc to 9 of these dogs (0.04 mg/kg of body weight every third day, n = 2; 0.08 mg/kg every other day, n = 2; and 0.08 mg/kg daily, n = 5), beginning the day after administration of doxorubicin, in an attempt to find an optimal dosage and schedule of administration of besm to reduce the duration and severity of chemotherapy-ind ... | 1992 | 1280926 |
| structure of a neutral glycan from the lipopolysaccharides of reference strains for serratia marcescens serogroups o2 and o3. | serogroups o2 and o3 of serratia marcescens are differentiated by acidic glycans present in the aqueous phase when lipopolysaccharides are extracted from the reference strains by the aqueous-phenol method. the phenolic phases of these extracts from both strains also contain lipopolysaccharides, from which the same neutral glycan is released on milk acid hydrolysis. the neutral glycan has the disaccharide repeating-unit shown, and accounts for the cross-reactions between the two serogroups and al ... | 1992 | 1283379 |
| rapid assessment of bacterial viability by flow cytometry. | the ability of a flow cytometer to rapidly assess microbial viability was investigated using three vital stains: rhodamine 123 (rh123); 3,3'-dihexyloxacarbocyanine iodide [dioc6(3)] and fluorescein diacetate (fda). rh123 was found to clearly differentiate viable from non-viable bacteria. the methodology for staining bacteria with this dye was optimised. rh123 was shown to stain and discriminate several different species of viable bacteria although this was not universal. viable cells of bacillus ... | 1992 | 1283525 |
| pseudobacteraemia and contaminated esr bottles. | | 1992 | 1285114 |
| a multicentre trial of cefaclor advanced formulation versus cefaclor in the treatment of acute bronchitis. | two prospective randomized, double-blind, parallel studies were carried out in europe to compare cefaclor advanced formulation (cefaclor af) with cefaclor in the treatment of acute bronchitis caused by susceptible pathogens. a total of 1,321 patients suffering from acute bronchitis confirmed by clinical data and a negative chest x-ray were randomized for treatment in the two multicentre trials. three doses of cefaclor af were tested: 375 mg twice daily and 500 mg twice daily were compared with c ... | 1992 | 1287614 |
| [microbicidal effect of lautercide, a new disinfectant]. | the authors tested the effect of a new disinfectant lautericide produced by qualichem co. neratovice which contains as the active agent acetate amine of coconut acid. for experiments standard methods were used and standard strains for testing of the following microbial strains: st. aureus, e. coli, ps. aeruginosa, proteus vulgaris, serratia marcescens, candida albicans, spores of bacillus subtilis, m. tuberculosis, m. fortuitum, m. avium. as a model virus e. coli bacteriophage ox 174 was used. i ... | 1992 | 1291115 |
| [changes in the content of reduced glutathione in the liver of mice under the action of staphylococcal enterotoxin type a and lipopolysaccharide]. | we investigated the level of reduced glutathione in mouse liver after injection of staphylococcal enterotoxin type a (sea) and serratia marcescens endotoxin (lps). it was shown that sea caused significant glutathione depletion. glutathione level fell more under the combined treatment by both toxins. the data demonstrate a considerable role of antioxidant potential in the mechanism of enhanced sensitivity of animals to the lethal effect produced by lps. | 1992 | 1292697 |
| inhibition of salmonella ferlac by enterobacteria under in-vitro conditions. | interactions of proteus morganii, proteus mirabilis, proteus sp. klebsiella oxaenae and serratia marcescens isolated from vegetable salads of mass feeding systems with salmonella ferlac (a new subgenus vi of salmonella) isolated from a hostel cook's hands and lizard droppings were recorded following in-vitro nephelometric analysis. nephelometric analysis revealed inhibition of s. ferlac by all the tested isolates from fifth hour of mixed culture interaction. k. oxaenae was the strong inhibitor. | 1992 | 1296949 |
| bacteriuria in patients with glomerular diseases. | in a comparative study of significant bacteriuria in an african population, 1.7% of 697 healthy subjects (10 females and 2 males) were found to have positive urine cultures. of these, 5 subjects grew e. coli, 4 klebsiella strains, 2 staphylococcus aureus and 1 serratia marcescens. among 116 patients with glomerular disease, 15.5% (7 males and 11 females) yielded positive cultures. e. coli, staph. aureus and proteus species were commonly isolated organisms. there was a nine fold increase in preva ... | 1992 | 1298612 |
| [keratitis associated with contact lens wear]. | keratitis associated with contact lens wear in 50 eyes (37 patients) was analyzed. the results revealed that lenses of extended-wear induced more cases of keratitis than did lenses of daily-wear. the pathogenetic mechanism was discussed. | 1992 | 1299570 |
| [genes of resistance to aminoglycoside antibiotics in clinical strains of serratia marcescens and the enzyme encoded by them]. | the patterns of aminoglycoside inactivating enzymes were determined by agrp in 31 clinical isolated of serratia marcescens. the results were compared with the data on identification of the aminoglycoside resistance genes by the specific dna probes. it was shown that all the isolates of serratia marcescens contained the aac(6')-ic gene which was not expressed in some isolates. the other detected aminoglycoside inactivating enzymes were the following: aac(3)-v in 17 isolates, ant(2'') in 7 isolate ... | 1992 | 1300924 |
| [the isolation of nucleases sm1 and sm2 in preparative amounts and a study of their antigenicity]. | two enzyme forms of endonuclease (sm 1 and sm 2) strain b10m1 in 60 and 100 mg respectively have been isolated from the culture fluid serratia marcescens. the chromatographic and electrophoretic properties and n-terminal amino acid residues are different for both enzymes. the purification procedure consists of dialysis and ion-exchange chromatography on deae- and phosphocellulose. the yield of nucleases sm1 and sm2 are 14% and 28% respectively. the antigenic differences of nucleases sm1 and sm2 ... | 1992 | 1302504 |
| [the comparative characteristics of the biological and immunomodulating activities of enzyme preparations of microbial origin]. | a number of enzyme preparations of microbial origin such as immunomodulators and immunostimulators have been studied in vitro according to reactions of rosette technique of cell (roc) active and general, adhesion, activation of a complement and also the spontaneous activity and phagocytosis of neutrophils have been determined. the enzyme preparations of microbial origin have been shown to be capable of causing immunomodulation and of influencing the specific and nonspecific immune response. unli ... | 1992 | 1302508 |