| the accessory genome of pseudomonas aeruginosa. | pseudomonas aeruginosa strains exhibit significant variability in pathogenicity and ecological flexibility. such interstrain differences reflect the dynamic nature of the p. aeruginosa genome, which is composed of a relatively invariable "core genome" and a highly variable "accessory genome." here we review the major classes of genetic elements comprising the p. aeruginosa accessory genome and highlight emerging themes in the acquisition and functional importance of these elements. although the ... | 2010 | 21119020 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| natural and engineered hydroxyectoine production based on the pseudomonas stutzeri ectabcd-ask gene cluster. | we report on the presence of a functional hydroxyectoine biosynthesis gene cluster, ectabcd-ask, in pseudomonas stutzeri dsm5190(t) and evaluate the suitability of p. stutzeri dsm5190(t) for hydroxyectoine production. furthermore, we present information on heterologous de novo production of the compatible solute hydroxyectoine in escherichia coli. in this host, the p. stutzeri gene cluster remained under the control of its salt-induced native promoters. we also noted the absence of trehalose whe ... | 2010 | 21169432 |
| bacterial communities associated with the lichen symbiosis. | lichens are commonly described as a mutualistic symbiosis between fungi and "algae" (chlorophyta or cyanobacteria); however, they also have internal bacterial communities. recent research suggests that lichen-associated microbes are an integral component of lichen thalli and that the classical view of this symbiotic relationship should be expanded to include bacteria. however, we still have a limited understanding of the phylogenetic structure of these communities and their variability across li ... | 2010 | 21169444 |
| bacterial communities associated with the lichen symbiosis. | lichens are commonly described as a mutualistic symbiosis between fungi and "algae" (chlorophyta or cyanobacteria); however, they also have internal bacterial communities. recent research suggests that lichen-associated microbes are an integral component of lichen thalli and that the classical view of this symbiotic relationship should be expanded to include bacteria. however, we still have a limited understanding of the phylogenetic structure of these communities and their variability across li ... | 2010 | 21169444 |
| identification of the monooxygenase gene clusters responsible for the regioselective oxidation of phenol to hydroquinone in mycobacteria. | mycobacterium goodii strain 12523 is an actinomycete that is able to oxidize phenol regioselectively at the para position to produce hydroquinone. in this study, we investigated the genes responsible for this unique regioselective oxidation. on the basis of the fact that the oxidation activity of m. goodii strain 12523 toward phenol is induced in the presence of acetone, we first identified acetone-induced proteins in this microorganism by two-dimensional electrophoretic analysis. the n-terminal ... | 2010 | 21183637 |
| identification of the monooxygenase gene clusters responsible for the regioselective oxidation of phenol to hydroquinone in mycobacteria. | mycobacterium goodii strain 12523 is an actinomycete that is able to oxidize phenol regioselectively at the para position to produce hydroquinone. in this study, we investigated the genes responsible for this unique regioselective oxidation. on the basis of the fact that the oxidation activity of m. goodii strain 12523 toward phenol is induced in the presence of acetone, we first identified acetone-induced proteins in this microorganism by two-dimensional electrophoretic analysis. the n-terminal ... | 2010 | 21183637 |
| characterization of helicobacter pylori factors that control transformation frequency and integration length during inter-strain dna recombination. | helicobacter pylori is a genetically diverse bacterial species, owing in part to its natural competence for dna uptake that facilitates recombination between strains. inter-strain dna recombination occurs during human infection and the h. pylori genome is in linkage equilibrium worldwide. despite this high propensity for dna exchange, little is known about the factors that limit the extent of recombination during natural transformation. here, we identify restriction-modification (r-m) systems as ... | 2010 | 21219459 |
| characterization of helicobacter pylori factors that control transformation frequency and integration length during inter-strain dna recombination. | helicobacter pylori is a genetically diverse bacterial species, owing in part to its natural competence for dna uptake that facilitates recombination between strains. inter-strain dna recombination occurs during human infection and the h. pylori genome is in linkage equilibrium worldwide. despite this high propensity for dna exchange, little is known about the factors that limit the extent of recombination during natural transformation. here, we identify restriction-modification (r-m) systems as ... | 2010 | 21219459 |
| nifb and nifen protein levels are regulated by clpx2 under nitrogen fixation conditions in azotobacter vinelandii. | the major part of biological nitrogen fixation is catalysed by the molybdenum nitrogenase that carries at its active site the iron and molybdenum cofactor (femo-co). the nitrogen fixation (nif) genes required for the biosynthesis of femo-co are derepressed in the absence of a source of fixed nitrogen. the nifb gene product is remarkable because it assembles nifb-co, a complex cluster proposed to comprise a [6fe-9s-x] cluster, from simpler [fe-s] clusters common to other metabolic pathways. nifb- ... | 2011 | 21231969 |
| pentachlorophenol degradation by pseudomonas stutzeri cl7 in the secondary sludge of pulp and paper mill. | a pentachlorophenol (pcp) mineralizing bacterium was isolated from the secondary sludge of pulp and paper mill and identified as pseudomonas stutzeri strain cl7. this isolate used pcp as its sole source of carbon and energy and was capable of degrading this compound as indicated by stoichiometric release of chloride and biomass formation. p. stutzeri (cl7) was able to mineralize a high concentration of pcp (600 mg/l) than any previously reported pseudomonad with pcp as sole carbon source. as the ... | 2010 | 21235193 |
| solution structure of 4'-phosphopantetheine - gmacp3 from geobacter metallireducens: a specialized acyl carrier protein with atypical structural features and a putative role in lipopolysaccharide biosynthesis. | gmacp3 from geobacter metallireducens is a specialized acyl carrier protein (acp) whose gene, gmet_2339, is located near genes encoding many proteins involved in lipopolysaccharide (lps) biosynthesis, indicating a likely function for gmacp3 in lps production. by overexpression in escherichia coli, about 50% holo-gmacp3 and 50% apo-gmacp3 were obtained. apo-gmacp3 exhibited slow precipitation and non-monomeric behavior by (15)n nmr relaxation measurements. addition of 4'-phosphopantetheine (4'-pp ... | 2011 | 21235239 |
| structural and functional studies of the escherichia coli phenylacetyl-coa monooxygenase complex. | the utilization of phenylacetic acid (pa) in escherichia coli occurs through a hybrid pathway that shows features of both aerobic and anaerobic metabolism. oxygenation of the aromatic ring is performed by a multisubunit phenylacetyl-coenzyme a oxygenase complex that shares remote homology of two subunits to well studied bacterial multicomponent monooxygenases and was postulated to form a new bacterial multicomponent monooxygenase subfamily. we expressed the subunits paaa, b, c, d, and e of the p ... | 2011 | 21247899 |
| application of nitroarene dioxygenases in the design of novel strains that degrade chloronitrobenzenes. | widespread application of chloronitrobenzenes as feedstocks for the production of industrial chemicals and pharmaceuticals has resulted in extensive environmental contamination with these toxic compounds, where they pose significant risks to the health of humans and wildlife. while biotreatment in general is an attractive solution for remediation, its effectiveness is limited with chloronitrobenzenes due to the small number of strains that can effectively mineralize these compounds and their abi ... | 2009 | 21261918 |
| isolation and characterization of "pseudomonas andersonii" from four cases of pulmonary granulomas and emended species description. | "pseudomonas andersonii" is a gram-negative bacillus initially isolated from a granulomatous lung lesion. novel species status has not been validated for this single strain. we report four additional cases of pulmonary granuloma involving p. andersonii and further characterize the organism. these patients had pulmonary nodules that were surgically resected and which grew p. andersonii on routine culture. mycobacterium avium complex was concomitantly isolated in two cases, and fungal structures w ... | 2011 | 21270229 |
| role of microbial exopolymeric substances (eps) on chromium sorption and transport in heterogeneous subsurface soils: i. cr(iii) complexation with eps in aqueous solution. | chromium (iii) binding by exopolymeric substances (eps) isolated from pseudomonas putida p18, pseudomonas aeruginosa p16 and pseudomonas stutzeri p40 strains were investigated by the determination of conditional stability constants and the concentration of functional groups using the ion-exchange experiments and potentiometric titrations. spectroscopic (exafs) analysis was also used to obtain information on the nature of cr(iii) binding with eps functional groups. the data from ion-exchange expe ... | 2011 | 21272912 |
| rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis. | we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ... | 2011 | 21289149 |
| biodegradation mechanism of biphenyl by a strain of pseudomonas stutzeri. | the mechanism of biphenyl biodegradation by pseudomonas stutzeri was studied. growth curves on biphenyl were measured, along with dissolution kinetics of biphenyl and production of biosurfactants by the bacterium. biphenyl was supplied as pure crystals (the crystal biphenyl), adsorbed to chromosorb g (the chromosorb g biphenyl) or xad-4 particles (the xad-4 biphenyl). no lag phase was observed on the crystal biphenyl, while this period lasted 3.5-6.0 hours on the chromosorb g biphenyl. the linea ... | 2011 | 21337247 |
| c-type lectin in chlamys farreri (cflec-1) mediating immune recognition and opsonization. | c-type lectins are a superfamily of ca(2+) dependent carbohydrate-recognition proteins that play significant diverse roles in nonself-recognition and clearance of invaders. though they are well characterized in vertebrates, the study of the potential function and mechanism of c-type lectins in invertebrate immunity is still in its infancy. | 2011 | 21347232 |
| root hairs play a key role in the endophytic colonization of olive roots by pseudomonas spp. with biocontrol activity. | the use of indigenous bacterial root endophytes with biocontrol activity against soil-borne phytopathogens is an environmentally-friendly and ecologically-efficient action within an integrated disease management framework. the earliest steps of olive root colonization by pseudomonas fluorescens picf7 and pseudomonas putida picp2, effective biocontrol agents (bcas) against verticillium wilt of olive (olea europaea l.) caused by the fungus verticillium dahliae kleb., are here described. a gnotobio ... | 2011 | 21347721 |
| molecular epidemiology of a pseudomonas aeruginosa hospital outbreak driven by a contaminated disinfectant-soap dispenser. | pseudomonas aeruginosa infection represents a main cause of morbidity and mortality among immunocompromised patients. this study describes a fatal epidemic of p. aeruginosa that occurred in a hematology unit in italy. | 2011 | 21359222 |
| multiple roles of component proteins in bacterial multicomponent monooxygenases: phenol hydroxylase and toluene/o-xylene monooxygenase from pseudomonas sp. ox1. | phenol hydroxylase (ph) and toluene/o-xylene monooxygenase (tomo) from pseudomonas sp. ox1 require three or four protein components to activate dioxygen for the oxidation of aromatic substrates at a carboxylate-bridged diiron center. in this study, we investigated the influence of the hydroxylases, regulatory proteins, and electron-transfer components of these systems on substrate (phenol; nadh) consumption and product (catechol; h(2)o(2)) generation. single-turnover experiments revealed that on ... | 2011 | 21366224 |
| neisseria meningitidis is structured in clades associated with restriction modification systems that modulate homologous recombination. | molecular data on a limited number of chromosomal loci have shown that the population of neisseria meningitidis (nm), a deadly human pathogen, is structured in distinct lineages. given that the nm population undergoes substantial recombination, the mechanisms resulting in the evolution of these lineages, their persistence in time, and the implications for the pathogenicity of the bacterium are not yet completely understood. based on whole-genome sequencing, we show that nm is structured in phylo ... | 2011 | 21368196 |
| characterisation of the putative effector interaction site of the regulatory hbpr protein from pseudomonas azelaica by site-directed mutagenesis. | bacterial transcription activators of the xylr/dmpr subfamily exert their expression control via ¤â(54)-dependent rna polymerase upon stimulation by a chemical effector, typically an aromatic compound. where the chemical effector interacts with the transcription regulator protein to achieve activation is still largely unknown. here we focus on the hbpr protein from pseudomonas azelaica, which is a member of the xylr/dmpr subfamily and responds to biaromatic effectors such as 2-hydroxybiphenyl. w ... | 2011 | 21379585 |
| non-identity-mediated crispr-bacteriophage interaction mediated via the csy and cas3 proteins. | studies of the escherichia, neisseria, thermotoga, and mycobacteria clustered regularly interspaced short palindromic repeat (crispr) subtypes have resulted in a model whereby crisprs function as a defense system against bacteriophage infection and conjugative plasmid transfer. in contrast, we previously showed that the yersinia-subtype crispr region of pseudomonas aeruginosa strain ucbpp-pa14 plays no detectable role in viral immunity but instead is required for bacteriophage dms3-dependent inh ... | 2011 | 21398535 |
| power plays: iron transport and energy transduction in pathogenic vibrios. | the vibrios are a unique group of bacteria inhabiting a vast array of aquatic environments. many vibrio species are capable of infecting a wide assortment of hosts. some of these species include v. parahaemolyticus, v. alginolyticus, v. vulnificus, v. anguillarum, and v. cholerae. the ability of these organisms to utilize iron is essential in establishing both an infection in their hosts as well as surviving in the environment. bacteria are able to sequester iron through the secretion of low mol ... | 2011 | 21399938 |
| evidence that wapb is a 1,2-glucosyltransferase of pseudomonas aeruginosa involved in lipopolysaccharide outer core biosynthesis. | pseudomonas aeruginosa is an important opportunistic pathogen infecting debilitated individuals. one of the major virulence factors expressed by p. aeruginosa is lipopolysaccharide (lps), which is composed of lipid a, core oligosaccharide (os), and o-antigen polysaccharide. the core os is divided into inner and outer regions. although the structure of the outer core os has been elucidated, the functions and mechanisms of the glycosyltransferases involved in core os biogenesis are currently unkno ... | 2011 | 21441506 |
| acetogens and acetoclastic methanosarcinales govern methane formation in abandoned coal mines. | in abandoned coal mines, methanogenic archaea are responsible for the production of substantial amounts of methane. the present study aimed to directly unravel the active methanogens mediating methane release as well as active bacteria potentially involved in the trophic network. therefore, the stable-isotope-labeled precursors of methane, [(13)c]acetate and h(2)-(13)co(2), were fed to liquid cultures from hard coal and mine timber from a coal mine in germany. guided by methane production rates, ... | 2011 | 21460109 |
| genomic analysis of the type vi secretion systems in pseudomonas spp.: novel clusters and putative effectors uncovered. | bacteria encode multiple protein secretion systems that are crucial for interaction with the environment and with hosts. in recent years, attention has focused on type vi secretion systems (t6sss), which are specialized transporters widely encoded in proteobacteria. the myriad of processes associated with these secretion systems could be explained by subclasses of t6ss, each involved in specialized functions. to assess diversity and predict function associated with different t6sss, comparative g ... | 2011 | 21474537 |
| nirk and nirs nitrite reductase genes from non-agricultural forest soil bacteria in thailand. | the genetic heterogeneity of the nitrite reductase gene (nirk and nirs) fragments from denitrifying prokaryotes in a non-agricultural forest soil in thailand was investigated using soil samples from the plant germplasm-royal initiation project area in kanchanaburi province, thailand. soil bacteria were screened for denitrification activity and 13 (from 211) positive isolates were obtained and further evaluated for their ability to reduce nitrate and to accumulate or reduce nitrite. three species ... | 2011 | 21475726 |
| nirk and nirs nitrite reductase genes from non-agricultural forest soil bacteria in thailand. | the genetic heterogeneity of the nitrite reductase gene (nirk and nirs) fragments from denitrifying prokaryotes in a non-agricultural forest soil in thailand was investigated using soil samples from the plant germplasm-royal initiation project area in kanchanaburi province, thailand. soil bacteria were screened for denitrification activity and 13 (from 211) positive isolates were obtained and further evaluated for their ability to reduce nitrate and to accumulate or reduce nitrite. three species ... | 2011 | 21475726 |
| engineering of polyhydroxyalkanoate (pha) synthase phac2(ps) of pseudomonas stutzeri via site-specific mutation for efficient production of pha copolymers. | the site-specific mutagenesis for pha synthase phac2(ps1317) from pseudomonas stutzeri 1317 was conducted for optimizing production of short-chain-length and medium-chain-length polyhydroxyalkanoates (scl-mcl pha). recombinant ralstonia eutropha phb-4 harboring double mutated phac2 ( ps1317 ) gene (phac2 ( ps ) qkst) produced 42 wt.% pha content in the cell dry weight (cdw) with 93 mol% 3-hydroxybutyrate (hb) as monomer in the pha copolymer. compared to that of wild-type phac2 ( ps1317 ), the hi ... | 2011 | 21509565 |
| complete genome sequence of the nitrogen-fixing and rhizosphere-associated bacterium pseudomonas stutzeri strain dsm4166. | we present here the analysis of the whole genome sequence of pseudomonas stutzeri strain dsm4166, a diazotrophic isolate from the rhizosphere of a sorghum nutans cultivar. to our knowledge, this is the second one to be sequenced in p. stutzeri. the availability and analysis of the genome provides insight into the evolution of the nitrogen fixation property, and identification of rhizosphere competence traits required in interactions with host plants. | 2011 | 21515765 |
| insights into the different dioxygen activation pathways of methane and toluene monooxygenase hydroxylases. | the methane and toluene monooxygenase hydroxylases (mmoh and tmoh, respectively) have almost identical active sites, yet the physical and chemical properties of their oxygenated intermediates, designated p*, h(peroxo), q, and q* in mmoh and tomoh(peroxo) in a subclass of tmoh, tomoh, are substantially different. we review and compare the structural differences in the vicinity of the active sites of these enzymes and discuss which changes could give rise to the different behavior of h(peroxo) and ... | 2011 | 21517016 |
| bioinformatic characterization of the trimeric intracellular cation-specific channel protein family. | trimeric intracellular cation-specific (tric) channels are integral to muscle excitation-contraction coupling. tric channels provide counter-ionic flux when calcium is rapidly transported from intracellular stores to the cell cytoplasm. until recently, knowledge of the presence of these proteins was limited to animals. we analyzed the tric family and identified a profusion of prokaryotic family members with topologies and motifs similar to those of their eukaryotic counterparts. prokaryotic memb ... | 2011 | 21519847 |
| geobacter sulfurreducens cytochrome c peroxidases: electrochemical classification of catalytic mechanisms. | bacterial cytochrome c peroxidase (ccp) enzymes are diheme redox proteins that reduce hydrogen peroxide to water. they are canonically characterized by a peroxidatic (called l, for "low reduction potential") active site heme and a secondary heme (h, for "high reduction potential") associated with electron transfer, and an enzymatic activity that exists only when the h-heme is prereduced to the fe(ii) oxidation state. the prereduction step results in a conformational change at the active site its ... | 2011 | 21524102 |
| cellular and transcriptional response of pseudomonas stutzeri to quantum dots under aerobic and denitrifying conditions. | pseudomonas stutzeri was exposed to quantum dots (qds) with three different surface coatings (anionic polymaleic anhydride-alt-1-octadecene (pmao), cationic polyethylenimine (pei), and carboxyl qds) under both aerobic and anaerobic (denitrifying) conditions. under aerobic conditions, toxicity (assessed per growth inhibition) increased from pmao to carboxyl to pei qds. the positive charge of pei facilitated direct contact with negatively charged bacteria, which was verified by tem analysis. both ... | 2011 | 21526814 |
| prevalence of sos-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons. | abstract: | 2011 | 21529368 |
| analysis of the activation mechanism of pseudomonas stutzeri cytochrome c peroxidase through an electron transfer chain. | the activation mechanism of pseudomonas stutzeri cytochrome c peroxidase (ccp) was probed through the mediated electrochemical catalysis by its physiological electron donor, p. stutzeri cytochrome c-551. a comparative study was carried out, by performing assays with the enzyme in the resting oxidized state as well as in the mixed-valence activated form, using cyclic voltammetry and a pyrolytic graphite membrane electrode. in the presence of both the enzyme and hydrogen peroxide, the peak-like si ... | 2011 | 21547574 |
| culture-independent analysis of bacterial fuel contamination provides insights into the level of concordance with the standard industry practice of aerobic cultivation. | bacterial diversity in contaminated fuels has not been systematically investigated using cultivation-independent methods. the fuel industry relies on phenotypic cultivation-based contaminant identification, which may lack accuracy and neglect difficult-to-culture taxa. using industry practice aerobic cultivation, 16s rrna gene sequencing and strain genotyping, a collection of 152 unique contaminant isolates from 54 fuel samples was assembled, and demonstrated a dominance of pseudomonas (21%), bu ... | 2011 | 21602386 |
| patchwork assembly of nag-like nitroarene dioxygenase genes and 3-chlorocatechol degradation cluster for the pathway evolution of 2-chloronitrobenzene catabolism in pseudomonas stutzeri zwlr2-1. | pseudomonas stutzeri zwlr2-1 utilizes 2-chloronitrobenzene (2cnb) as a sole source of carbon, nitrogen and energy. to identify genes involved in this pathway, a 16.2-kb dna fragment containing putative 2-chloronitrobenzene dioxygenase genes was cloned and sequenced. of the products from the 19 open reading frames that resulted from this fragment, cnbac and cnbad exhibited striking identities to the respective α- and β- subunits of the nag-like ring-hydroxylating dioxygenases involved in the meta ... | 2011 | 21602392 |
| comparative activities of tigecycline and other tetracyclines against non-fermenting gram-negative bacilli (excluding acinetobacter spp.). | tigecycline, a glycylcycline, is a semisynthetic derivative of minocycline with a broad spectrum of activity against aerobic and anaerobic bacteria (2, 12, 16).… | 2011 | 21606223 |
| the aftermath of the western australian melioidosis outbreak. | abstract. melioidosis became a notifiable disease in western australia (wa) 2 years after the west kimberley melioidosis outbreak. two cases of melioidosis caused by the outbreak genotype of burkholderia pseudomallei (national collection of type cultures [nctc] 13177) occurred in 1998 and 1999 in persons who visited the outbreak location at the time. no other infections caused by the outbreak strain have been recorded in wa since that time, despite an average of four culture-positive cases per y ... | 2011 | 21633018 |
| a sira-like gene, sira2, is essential for 3-succinoyl-pyridine metabolism in the newly isolated nicotine-degrading pseudomonas sp. hzn6 strain. | a novel nicotine-degrading pseudomonas sp. strain, hzn6, was isolated from a pesticide-wastewater treatment facility in hangzhou. the strain could grow on nicotine as its sole source of carbon, nitrogen, and energy. the strain's main intermediate metabolites were determined to be pseudooxynicotine, 3-succinoyl-pyridine (sp), and 6-hydroxy-3-succinoyl-pyridine (hsp). a tn5 transposon mutant was generated in which the degradation pathway was blocked at the sp. a 4,583-bp dna fragment flanking the ... | 2011 | 21637938 |
| the burden of invasive early-onset neonatal sepsis in the united states, 2005-2008. | background:: sepsis in the first 3 days of life is a leading cause of morbidity and mortality among infants. group b streptococcus (gbs), historically the primary cause of early-onset sepsis (eos), has declined through widespread use of intrapartum chemoprophylaxis. we estimated the national burden of invasive eos cases and deaths in the era of gbs prevention. methods:: population-based surveillance for invasive eos was conducted in 4 of the centers for disease control and prevention's active ba ... | 2011 | 21654548 |
| genomic analysis reveals versatile organisms for quorum quenching enzymes: acyl-homoserine lactone-acylase and -lactonase. | microbial virulence and their resistance to multiple drugs have obliged researchers to look for novel drug targets. virulence of pathogenic microbes is regulated by signal molecules such as acylated homoserine lactone (ahl) produced during a cell density dependent phenomenon of quorum sensing (qs). in contrast, certain microbes produce ahl-lactonases and -acylases to degrade qs signals, also termed as quorum quenching. mining sequenced genome databases has revealed organisms possessing conserved ... | 2011 | 21660112 |
| defining the pseudomonas genus: where do we draw the line with azotobacter? | the genus pseudomonas has gone through many taxonomic revisions over the past 100 years, going from a very large and diverse group of bacteria to a smaller, more refined and ordered list having specific properties. the relationship of the pseudomonas genus to azotobacter vinelandii is examined using three genomic sequence-based methods. first, using 16s rrna trees, it is shown that a. vinelandii groups within the pseudomonas close to pseudomonas aeruginosa. genomes from other related organisms ( ... | 2011 | 21811795 |
| defining the pseudomonas genus: where do we draw the line with azotobacter? | the genus pseudomonas has gone through many taxonomic revisions over the past 100 years, going from a very large and diverse group of bacteria to a smaller, more refined and ordered list having specific properties. the relationship of the pseudomonas genus to azotobacter vinelandii is examined using three genomic sequence-based methods. first, using 16s rrna trees, it is shown that a. vinelandii groups within the pseudomonas close to pseudomonas aeruginosa. genomes from other related organisms ( ... | 2011 | 21811795 |
| protein secretion systems in pseudomonas aeruginosa: an essay on diversity, evolution, and function. | protein secretion systems are molecular nanomachines used by gram-negative bacteria to thrive within their environment. they are used to release enzymes that hydrolyze complex carbon sources into usable compounds, or to release proteins that capture essential ions such as iron. they are also used to colonize and survive within eukaryotic hosts, causing acute or chronic infections, subverting the host cell response and escaping the immune system. in this article, the opportunistic human pathogen ... | 2011 | 21811488 |
| effects of photoactivated titanium dioxide nanopowders and coating on planktonic and biofilm growth of pseudomonas aeruginosa. | we exploited the ability of photocatalytic titanium dioxide (tio(2) ) as an agent for the biofilm control. two photocatalytic systems were investigated: a 3g/l suspension of tio(2) nanopowder in demineralised water and glass slides coated with a tio(2) thin film, achieved by sol-gel deposition. a running protocol for the photoactivation of tio(2) was set up using the dye rhodamine b. the microorganisms studied were pseudomonas stutzeri, pseudomonas aeruginosa and a bacillus cereus-group as plank ... | 2011 | 21790617 |
| rapid concentration and molecular enrichment approach for sensitive detection of escherichia coli/shigella in potable water samples. | in this work, we used a rapid, simple, and efficient concentration and recovery procedure combined with a dna enrichment method (dubbed crename), that we coupled to an escherichia coli/shigella-specific real-time pcr (rtpcr) assay targeting the tuf gene, to sensitively detect e. coli/shigella in water. this integrated method was compared to the usepa culture-based method 1604 on mi agar in terms of analytical specificity, ubiquity, detection limit, and rapidity. none of the 179 non-e. coli/shige ... | 2011 | 21764965 |
| ligands of thermophilic abc transporters encoded in a newly sequenced genomic region of thermotoga maritima msb8 screened by differential scanning fluorimetry. | the chromosome of thermotoga maritima strain msb8 was found to have a 8,870 bp region that is not present in its published sequence. the isolate that was sequenced by the institute for genomic research (tigr) in 1999 is apparently a laboratory variant of the isolate deposited at the deutsche sammlung von mikroorganismen und zellkulturen (dsm 3109) in 1986. this newly sequenced region from the dsmz culture was located between tm1848 (cbp, cellobiose phosphorylase) and tm1847 (the 3' end of a trun ... | 2011 | 21764944 |
| microfluidic fluorescence in situ hybridization and flow cytometry (µflowfish). | we describe an integrated microfluidic device (µflowfish) capable of performing 16s rrna fluorescence in situ hybridization (fish) followed by flow cytometric detection for identifying bacteria in natural microbial communities. the device was used for detection of species involved in bioremediation of cr(vi) and other metals in groundwater samples from a highly-contaminated environmental site (hanford, wa, usa). the µflowfish seamlessly integrates two components: a hybridization chamber formed b ... | 2011 | 21755095 |
| a new family of bacterial condensins. | condensins play a central role in global chromatin organization. in bacteria, two families of condensins have been identified, the mukbef and smc-scpab complexes. only one of the two complexes is usually found in a given species, giving rise to a paradigm that a single condensin organizes bacterial chromosomes. using sequence analysis, we identified a third family of condensins, mksbef (mukbef-like smc proteins), which is broadly present in diverse bacteria. the proteins appear distantly related ... | 2011 | 21752107 |
| a specialized aspartokinase enhances the biosynthesis of the osmoprotectants ectoine and hydroxyectoine in pseudomonas stutzeria1501. | the compatible solutes ectoine and hydroxyectoine are widely produced by bacteria as protectants against osmotic and temperature stress. l-aspartate-beta-semialdehyde is used as the precursor molecule for ectoine/hydroxyectoine biosynthesis that is catalyzed by the ectabcd enzymes. l-aspartate-beta-semialdehyde is a central intermediate in different biosynthetic pathways and is produced from l-aspartate by aspartokinase (ask) and aspartate-semialdehyde-dehydrogenase (asd). ask activity is typica ... | 2011 | 21725014 |
| virulence determinants, drug resistance and mobile genetic elements of laribacter hongkongensis: a genome-wide analysis. | | 2011 | 21711902 |
| characterization of pseudomonas stutzeri nt-i capable of removing soluble selenium from the aqueous phase under aerobic conditions. | pseudomonas stutzeri strain nt-i was isolated from the drainage wastewater of a selenium refinery plant. this bacterium efficiently reduced selenate to elemental selenium without prolonged accumulation of selenite under aerobic conditions. strain nt-i was able to reduce selenate completely at high concentrations (up to 10mm) and selenite almost completely (up to 9mm). in addition, higher concentrations of selenate and selenite were substantially reduced. activity was observed under the following ... | 2011 | 21676651 |
| genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere. | the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ... | 2011 | 21686169 |
| co-expression of an organic solvent-tolerant lipase and its cognate foldase of pseudomonas aeruginosa cs-2 and the application of the immobilized recombinant lipase. | the genes of cs-2 lipase and its cognate foldase were cloned from pseudomonas aeruginosa cs-2. a stop codon was not found in the lipase gene. the amino acid sequence deduced from the lipase gene from p. aeruginosa cs-2 showed 97.8%, 71.3%, and 71.2% identity with lipases from p. aeruginosa lst-03, p seudomonas mendocina ymp, and pseudomonas stutzeri a1501, respectively. the co-expression of cs-2 lipase and its cognate foldase of p. aeruginosa cs-2 in e scherichia coli bl21 (de3) resulted in the ... | 2011 | 21720839 |
| n2o binding at a [4cu:2s] copper-sulphur cluster in nitrous oxide reductase. | nitrous oxide (n(2)o) is generated by natural and anthropogenic processes and has a critical role in environmental chemistry. it has an ozone-depleting potential similar to that of hydrochlorofluorocarbons as well as a global warming potential exceeding that of co(2) 300-fold. in bacterial denitrification, n(2)o is reduced to n(2) by the copper-dependent nitrous oxide reductase (n(2)or). this enzyme carries the mixed-valent cu(a) centre and the unique, tetranuclear cu(z) site. previous structura ... | 2011 | 21841804 |
| kinetics of styrene biodegradation by pseudomonas sp. e-93486. | the research into kinetics of styrene biodegradation by bacterial strain pseudomonas sp. e-93486 coming from vtt culture collection (finland) was presented in this work. microbial growth tests in the presence of styrene as the sole carbon and energy source were performed both in batch and continuous cultures. batch experiments were conducted for initial concentration of styrene in the liquid phase changed in the range of 5-90-ágôçëm(-3). the haldane model was found to be the best to fit the kine ... | 2011 | 21833566 |
| kinetics of styrene biodegradation by pseudomonas sp. e-93486. | the research into kinetics of styrene biodegradation by bacterial strain pseudomonas sp. e-93486 coming from vtt culture collection (finland) was presented in this work. microbial growth tests in the presence of styrene as the sole carbon and energy source were performed both in batch and continuous cultures. batch experiments were conducted for initial concentration of styrene in the liquid phase changed in the range of 5-90-ágôçëm(-3). the haldane model was found to be the best to fit the kine ... | 2011 | 21833566 |
| horizontal gene exchange in environmental microbiota. | horizontal gene transfer (hgt) plays an important role in the evolution of life on the earth. this view is supported by numerous occasions of hgt that are recorded in the genomes of all three domains of living organisms. hgt-mediated rapid evolution is especially noticeable among the bacteria, which demonstrate formidable adaptability in the face of recent environmental changes imposed by human activities, such as the use of antibiotics, industrial contamination, and intensive agriculture. at th ... | 2011 | 21845185 |
| metabolic engineering of aeromonas hydrophila 4ak4 for production of copolymers of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoate. | a mutant termed aeromonas hydrophila aklf was constructed by deleting acetic acid pathway related genes pta and acka in a. hydrophila 4ak4. accumulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) in a. hydrophila aklf was increased by 47% from 2.11 to 3.10g/l associated with a reduction on acetic acid formation compared with a. hydrophila 4ak4 when lauric acid was used as carbon resource. a. hydrophila aklf harboring pvgab encoding vitreoscilla hemoglobin, +¦-ketothiolase and acet ... | 2011 | 21680176 |
| emerging agents to combat complicated and resistant infections: focus on ceftobiprole. | antimicrobial resistance is a global concern. over the past few years, considerable efforts and resources have been expended to detect, monitor, and understand at the basic level the many different facets of emerging and increasing resistance. development of new antimicrobial agents has been matched by the development of new mechanisms of resistance by bacteria. current antibiotics act at a variety of sites within the target bacteria, including the cross-linking enzymes in the cell wall, various ... | 2010 | 21694889 |
| within-genome evolution of repins: a new family of miniature mobile dna in bacteria. | repetitive sequences are a conserved feature of many bacterial genomes. while first reported almost thirty years ago, and frequently exploited for genotyping purposes, little is known about their origin, maintenance, or processes affecting the dynamics of within-genome evolution. here, beginning with analysis of the diversity and abundance of short oligonucleotide sequences in the genome of pseudomonas fluorescens sbw25, we show that over-represented short sequences define three distinct groups ... | 2011 | 21698139 |
| regulation and function of versatile aerobic and anaerobic respiratory metabolism in pseudomonas aeruginosa. | pseudomonas aeruginosa is a ubiquitously distributed opportunistic pathogen that inhabits soil and water as well as animal-, human-, and plant-host-associated environments. the ubiquity would be attributed to its very versatile energy metabolism. p. aeruginosa has a highly branched respiratory chain terminated by multiple terminal oxidases and denitrification enzymes. five terminal oxidases for aerobic respiration have been identified in the p. aeruginosa cells. three of them, the cbb(3)-1 oxida ... | 2011 | 21833336 |
| surface-induced intramolecular electron transfer in multi-centre redox metalloproteins: the di-haem protein cytochrome c(4) in homogeneous solution and at electrochemical surfaces. | intramolecular electron transfer (et) between transition metal centres is a core feature of biological et and redox enzyme function. the number of microscopic redox potentials and et rate constants is, however, mostly prohibitive for experimental mapping, but two-centre proteins offer simple enough communication networks for complete mapping to be within reach. at the same time, multi-centre redox proteins operate in a membrane environment where conformational dynamics and et patterns are quite ... | 2008 | 21694431 |
| expression of the nos operon proteins from pseudomonas stutzeri in transgenic plants to assemble nitrous oxide reductase. | nitrous oxide (n(2)o) is a stable greenhouse gas that plays a significant role in the destruction of the ozone layer. soils are a significant source of atmospheric n(2)o. it is important to explore some innovative and effective biology-based strategies for n(2)o mitigation. the enzyme nitrous oxide reductase (n(2)or), naturally found in soil bacteria, is responsible for catalysing the final step of the denitrification pathway, conversion of n(2)o to dintrogen gas (n(2)). to transfer this catalyt ... | 2011 | 21938458 |
| heterotrophic nitrification and aerobic denitrification by the bacterium pseudomonas stutzeri yzn-001. | a strain yzn-001 was isolated from swine manure effluent and was identified as pseudomonas stutzeri. it can utilise not only nitrate and nitrite, but also ammonium. the strain had the capability to fully remove as much as 275.08 mg l(-1) no(3)(-)-n and 171.40 mg l(-1) no(2)(-)-n under aerobic conditions. furthermore, at 30°c, the utilization of ammonium is approximately 95% by 18 h with a similar level removed by 72 h and 2 weeks at 10 and 4°c, respectively. triplicate sets of tightly sealed ser ... | 2011 | 21911288 |
| sequence of the hyperplastic genome of the naturally competent thermus scotoductus sa-01. | many strains of thermus have been isolated from hot environments around the world. thermus scotoductus sa-01 was isolated from fissure water collected 3.2 km below surface in a south african gold mine. the isolate is capable of dissimilatory iron reduction, growth with oxygen and nitrate as terminal electron acceptors and the ability to reduce a variety of metal ions, including gold, chromate and uranium, was demonstrated. the genomes from two different thermus thermophilus strains have been com ... | 2011 | 22115438 |
| A new sesquiterpenoid from the rhizomes of Homalomena sagittifolia. | A new sesquiterpenoid, 1a,4ß,7ß-eudesmanetriol (1), was isolated together with the known compounds 1ß,4ß,7ß-eudesmanetriol (2) and oplopanone (3) from the rhizomes of Homalomena sagittifolia. The structures of these compounds were determined by extensive spectral analyses. The compounds 1 and 2 inhibited growth of Pseudomonas stutzeri with a MIC value of 117?µM when evaluated for antibacterial activity using the minimum concentration assay. Both these compounds showed remarkable activities again ... | 2011 | 21999629 |
| Complete genome sequence of the type strain Pseudomonas stutzeri CGMCC 1.1803. | Here we report the complete genome sequence of Pseudomonas stutzeri strain CGMCC 1.1803 (equivalent to ATCC 17588), the type strain of P. stutzeri, which encodes 4,138 open reading frames on a 4,547,930-bp circular chromosome. The CGMCC 1.1803 genome contains genes involved in denitrification, benzoate/catechol degradation, chemotaxis, and other functions. | 2011 | 21994926 |
| Low Efficiency of Homology-Facilitated Illegitimate Recombination during Conjugation in Escherichia coli. | Homology-facilitated illegitimate recombination has been described in three naturally competent bacterial species. It permits integration of small linear DNA molecules into the chromosome by homologous recombination at one end of the linear DNA substrate, and illegitimate recombination at the other end. We report that homology-facilitated illegitimate recombination also occurs in Escherichia coli during conjugation with small non-replicative plasmids, but at a low frequency of 3×10(-10) per reci ... | 2011 | 22194937 |
| identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization sepsityper™ and time of flight mass spectrometry. | matrix-assisted laser desorption ionization time of flight mass spectrometry (maldi-tof ms) is a novel method for the direct identification of bacteria from blood culture broths. we evaluate for the first time, the performance of the maldi sepsityper™ kit and ms for the identification of bacteria compared to standard phenotypic methods using the manufacturer's specified bacterial identification criteria (spectral scores ≥1.700-1.999 and ≥2.000 indicated identification to genus and species level, ... | 2011 | 21858058 |
| Technoeconomic assessment of phenanthrene degradation by Pseudomonasstutzeri CECT 930 in a batch bioreactor. | Polycyclic aromatic hydrocarbons (PAHs) are among the most persistent pollutants that accumulate in natural environment mainly as a result of anthropogenic activities. Therefore, the improvement of the available bank of microbial resources and information is crucial to the proper management of PAHs-polluted sites and effluents. In this work, Pseudomonasstutzeri CECT 930 was selected for aerobically degrading an aqueous effluent containing phenanthrene (PHE). Maximum PHE degradation of 90% was ob ... | 2012 | 22074903 |
| Bacillus Strains Most Closely Related to Bacillus nealsonii Are Not Effectively Circumscribed within the Taxonomic Species Definition. | Bacillus strains with >99.7% 16S rRNA gene sequence similarity were characterized with DNA:DNA hybridization, cellular fatty acid (CFA) analysis, and testing of 100 phenotypic traits. When paired with the most closely related type strain, percent DNA:DNA similarities (% S) for six Bacillus strains were all far below the recommended 70% threshold value for species circumscription with Bacillus nealsonii. An apparent genomic group of four Bacillus strain pairings with 94%-70% S was contradicted by ... | 2011 | 22046187 |
| Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy. | Fixation ability of five common fixation solutions, including 2.5% glutaraldehyde, 10% formalin, 4% paraformaldehyde, methanol/acetone (1:1), and ethanol/acetic acid (3:1) were evaluated by using atomic force microscopy in the present study. Three model bacteria, i.e., Escherichia coli, Pseudomonas putida, and Bacillus subtilis were applied to observe the above fixation methods for the morphology preservation of bacterial cells and surface ultrastructures. All the fixation methods could effectiv ... | 2011 | 21881891 |
| characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat. | diazotrophic bacteria were isolated from the rhizosphere of field-grown triticum aestivum, hordeum vulgare, and avena sativa grown in various regions of greece. one isolate, with the highest nitrogen-fixation ability from each of the eleven rhizospheres, was selected for further characterisation. diazotrophic strains were assessed for plant-growth-promoting traits such as indoleacetic acid production and phosphate solubilisation. the phylogenies of 16s rrna gene of the selected isolates were com ... | 2011 | 21887633 |
| the potential for reductive mobilization of arsenic [as(v) to as(iii)] by osbh(2) (pseudomonas stutzeri) and osbh(5) (bacillus cereus) in an oil-contaminated site. | microbial reduction of arsenate [as(v)] plays an important role in arsenic (as) mobilization in aqueous environments. in this study, we investigated reduction of arsenate by different bacterial isolates such as osbh(1) (gu329913), osbh(2) (gu329914), osbh(3) (gu329915), osbh(4) (gu329916) and osbh(5) (gu329917), isolated from the oil sludge of a sewage treatment plant operated by the china petroleum refinery company in kaohsiung, southern taiwan. bacterial strains of pure culture were identified ... | 2011 | 21879856 |
| The effects of solid media type on routine identification of bacterial isolates using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. | Background: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis is a rapid method for the identification of bacteria based upon the comparison of protein profiles. Factors that may alter profiles include culture conditions and presence of exogenous substances and could hinder identification. Methods: Bacterial isolates identified using conventional methods were cultured on various media and identified using the MALDI Biotyper system (Bruker Dalton ... | 2011 | 22162546 |
| Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506. | Phosphite dehydrogenase (PtxD), which catalyzes the nearly irreversible oxidation of phosphite to phosphate with the concomitant reduction of NAD(+) to NADH, has great potential for NADH regeneration in industrial biocatalysts. Here, we isolated a soil bacterium, Ralstonia sp. strain 4506, that grew at 45°C on a minimal medium containing phosphite as the sole source of phosphorus. A recombinant PtxD of Ralstonia sp. (PtxD(R4506)) appeared in the soluble fraction in Escherichia coli. The purified ... | 2011 | 22197497 |
| assessment of denitrifying bacterial composition in activated sludge. | the abundance and structure of denitrifying bacterial community in different activated sludge samples were assessed, where the abundance of denitrifying functional genes showed nirs in the range of 10(4)-10(5), nosz with 10(4)-10(6) and 16s rrna gene in the range 10(9)-10(10) copy number per ml of sludge. the culturable approach revealed pseudomonas sp. and alcaligenes sp. to be numerically high, whereas culture independent method showed betaproteobacteria to dominate the sludge samples. comamon ... | 2011 | 21868215 |
| engineering the hyperthermophilic archaeon pyrococcus furiosus to overproduce its cytoplasmic [nife]-hydrogenase. | the cytoplasmic hydrogenase (shi) of the hyperthermophilic archaeon pyrococcus furiosus is a nadp(h)-dependent hetero-tetrameric enzyme that contains a nickel-iron catalytic site, flavin and six iron-sulfur clusters. it has potential utility in a range of bioenergy systems in vitro but a major obstacle in its use is generating sufficient amounts. we have engineered p. furiosus to overproduce shi utilizing a recently developed genetic system. in the over-expression oe-shi strain, transcription ... | 2011 | 22157005 |
| engineering the hyperthermophilic archaeon pyrococcus furiosus to overproduce its cytoplasmic [nife]-hydrogenase. | the cytoplasmic hydrogenase (shi) of the hyperthermophilic archaeon pyrococcus furiosus is a nadp(h)-dependent hetero-tetrameric enzyme that contains a nickel-iron catalytic site, flavin and six iron-sulfur clusters. it has potential utility in a range of bioenergy systems in vitro but a major obstacle in its use is generating sufficient amounts. we have engineered p. furiosus to overproduce shi utilizing a recently developed genetic system. in the over-expression oe-shi strain, transcription ... | 2011 | 22157005 |
| natural transformation with synthetic gene cassettes: new tools for integron research and biotechnology. | integrons are genetic elements that can capture and express genes packaged as gene cassettes. here we report new methods that allow integrons to be studied and manipulated in their native bacterial hosts. synthetic gene cassettes encoding gentamicin resistance (aadb) and green fluorescence (gfp), or lactose metabolism (laczy), were made by pcr and self-ligation, converted to large tandem arrays by multiple displacement amplification, and introduced into escherichia coli or pseudomonas stutzeri s ... | 2011 | 21948046 |
| investigation of microbial safety of a full-scale ozonation and biological activated carbon process under high humidity and temperature conditions. | microbial safety of a full-scale ozonation and biological activated carbon (bac) process was investigated by examining pathogens, microbial community and particle counts, with emphasis on the bac effluent. the process is located at south china, where the average humidity and air temperature were 70-80% and 22-24 °c, respectively. a high diversity of microbial community existed on the bac media. three types of dominant bacteria were identified, including chryseobacterium indologenes, bacillus bre ... | 2011 | 22156135 |
| adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers. | in this study, we examined the effect of polychlorinated biphenyls (pcbs) in the presence of natural and synthetic terpenes and biphenyl on biomass production, lipid accumulation, and membrane adaptation mechanisms of two pcb-degrading bacterial strains pseudomonas stutzeri and burkholderia xenovorans lb400. according to the results obtained, it could be concluded that natural terpenes, mainly those contained in ivy leaves and pine needles, decreased adaptation responses induced by pcbs in these ... | 2011 | 22159613 |
| use of biopolymers as solid substrates for denitrification. | the conventional process to remove nitrate from water, the biological denitrification, uses the addition of dissolved organic carbon that has the potential risk to further deteriorate water quality. thus, this work aimed to evaluate the specific denitrification activity of a mixed microbial culture and a pure culture of pseudomonas stutzeri with solid substrates such as polycaprolactone (pcl), polylactic acid (pla), and starch. the highest nitrate reduction activity was obtained with a microbial ... | 2011 | 22173413 |
| dual pathways for copper uptake by methanotrophic bacteria. | methanobactin (mb), a 1217-da copper chelator produced by the methanotroph methylosinus trichosporium ob3b, is hypothesized to mediate copper acquisition from the environment, particularly from insoluble copper mineral sources. although indirect evidence suggests that mb provides copper for the regulation and activity of methane monooxygenase enzymes, experimental data for direct uptake of copper loaded mb (cu-mb) are lacking. uptake of intact cu-mb by m. trichosporium ob3b was demonstrated by i ... | 2011 | 21900235 |
| evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders. | investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ... | 2011 | 22205979 |
| Assessment of a process to degrade metal working fluids using Pseudomonas stutzeri CECT 930 and indigenous microbial consortia. | The development of a novel biological process to treat metal working fluids (MWFs)-containing effluents at bioreactor scale was pursued in this work. The bacteria Pseudomonas stutzeri CECT 930 was investigated for the first time as an alternative agent for MWF degradation. An adequate medium design and mixing and aeration system, as well as an appropriate microorganism proved to be crucial for reaching high levels of degradation by P. stutzeri and by an indigenous consortium (about 70% and 50% o ... | 2011 | 22055314 |
| enrichment and characterization of an autotrophic ammonia-oxidizing archaeon of mesophilic crenarchaeal group i.1a from an agricultural soil. | soil nitrification is an important process for agricultural productivity and environmental pollution. though one cultivated representative of ammonia-oxidizing archaea from soil has been described, additional representatives warrant characterization. we describe an ammonia-oxidizing archaeon (strain my1) in a highly enriched culture derived from agricultural soil. fluorescence in situ hybridization microscopy showed that, after 2 years of enrichment, the culture was composed of >90% archaeal cel ... | 2011 | 22003023 |
| Immobilization of Pseudomonas stutzeri lipase for the transesterification of wood sterols with fatty acid esters. | Lipase from Pseudomonas stutzeri PL-836 was immobilized on hydrophobic supports and evaluated in the transesterification of wood sterols in solvent-free and solvent-containing media. Triton X-100 was used as additive during immobilization in butyl and octadecyl sepabeads increasing enzyme activity yield by 5% and 60%, respectively. Hyperactivation was observed during immobilization in EC octadecyl sepabeads with enzyme activity yield of 200% and protein immobilization yield of 93%. Thermostabili ... | 2011 | 21887523 |
| Effect of carbon source, C/N ratio, nitrate and dissolved oxygen concentration on nitrite and ammonium production from denitrification process by Pseudomonasstutzeri D6. | Pseudomonasstutzeri D6, selectively isolated from activated sludge was used to study NO(2)(-) and NH(4)(+) production from denitrification processes. Changes in carbon type, C/N ratio and oxygen concentration significantly influenced the magnitude of NO(2)(-) and NH(4)(+) accumulation through denitrification. D6 showed a preference for citrate and acetate, which led to the largest quantity of nitrate reduced and which were exhausted most rapidly, with minimal intermediate products accumulation. ... | 2012 | 22074905 |
| nonfermenting gram-negative bacilli infections in a tertiary care hospital in kolar, karnataka. | aim: nonfermenting gram-negative bacilli (nfgnb), which are saprophytic in nature, have emerged as important healthcare-associated pathogens. they exhibit resistance not only to beta lactam and the other groups of antibiotics, but also to carbapenems. this study was undertaken to identify the nonfermenters isolated from various clinical samples, to assess their clinical significance, to know the type of healthcare-associated infections they caused, and to know their anti-microbial sensitivity pa ... | 2009 | 21938252 |
| Cell surface properties of Pseudomonas stutzeri in the process of diesel oil biodegradation. | Pseudomonas stutzeri, isolated from crude oil-contaminated soil, was used to degrade diesel oil. Of three surfactants, 120 mg rhamnolipids 1(-1) significantly increased degradation of diesel oil giving 88% loss after 14 days compared to 54% loss without the surfactant. The system with rhamnolipids was characterised by relatively high particle homogeneity. However, the addition of saponins to diesel oil caused the cells to aggregate (the polydispersity index: 0.542) and the biodegradation of dies ... | 2011 | 22210557 |
| Comparative genomic analysis of Acinetobacter oleivorans DR1 to determine strain-specific genomic regions and gentisate biodegradation. | The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplet ... | 2011 | 21856821 |
| a scalable microfluidic chip for bacterial suspension culture. | microfluidic systems could, in principle, enable high-throughput breeding and screening of microbial strains for industrial applications, but parallel and scalable culture and detection chips are needed before complete microbial selection systems can be integrated and tested. here we demonstrate a scalable multi-channel chip that is capable of bacterial suspension culture. the key invention is a multi-layered chip design, which enables a single set of control channels to function as serial peris ... | 2011 | 22030862 |
| identification and characterization of a novel class of extracellular poly(3-hydroxybutyrate) depolymerase from bacillus sp. strain nrrl b-14911. | the catalytic, linker, and denatured poly(3-hydroxybutyrate) (dphb)-binding domains of bacterial extracellular phb depolymerases (phazs) are classified into several different types. we now report a novel class of extracellular phb depolymerase from bacillus sp. strain nrrl b-14911. its catalytic domain belongs to type 1, whereas its putative linker region neither possesses the sequence features of the three known types of linker domains nor exhibits significant amino acid sequence similarity to ... | 2011 | 21948827 |