| leukocyte migration-inhibition responses of nonvaccinated and vaccinated heifers to experimental infection with brucella abortus. | the leukocyte migration agarose technique was used to show the leukocyte migration-inhibition responses of nonvaccinated and vaccinated heifers to experimental infection with brucella abortus. all heifers had increased leukocyte migration inhibition after exposure to b abortus. nonvaccinated heifers which aborted had the highest responses. the responses of the vaccinated heifers were significantly (p less than 0.01) lower than those of nonvaccinated heifers. none of the vaccinated heifers aborte ... | 1982 | 6803624 |
| on the differentiation of "brucella abortus" biotypes 3 and 6. | | 1981 | 6803685 |
| the effects of ochratoxin a on the immune response of swiss mice. | the acute intraperitoneal toxicity of ochratoxin a (oa) for adult female swiss mice is presented. the seven-day ld(50) was calculated to be 48 +/- 3.2 mg/kg. daily intraperitoneal administrations of 10 mg oa/kg resulted in 50% mortality by the tenth day of injection. clinical symptoms included depression, huddling, roughened hair coats, humped backs and reduced weight gains. mice injected intraperitoneally daily for 50 days with 5 mg oa/kg had a significantly (p<0.01) depressed antibody response ... | 1982 | 6804072 |
| generation of chemotactic factor for granulocytes and monocytes from serum by fractions of brucella abortus. | several fractions isolated from brucella abortus were examined for their ability to generate chemotactic factor from normal serum. phenol phase lipopolysaccharides exhibited activity equivalent to that obtained with e. coli lipopolysaccharide. a carbohydrate-rich aqueous methanol fraction was inhibitory at high concentrations, but a non-dialysable component of this fraction contained a potent stimulator of chemotactic activity. protein-rich fractions from both strain 19 and strain 2308 were inac ... | 1982 | 6804374 |
| persistence of brucella abortus strain 19 infection in adult cattle vaccinated with reduced doses. | brucella abortus strain 19 was recovered at necropsy from cows vaccinated with reduced doses of strain 19, three to six months previously. strain 19 was isolated from four of nine cows vaccinated with 5.8 x 10(9) viable cells during pregnancy. in two of these nine cows vaccination had led to colonisation of the uterus with erythritol-utilising mutants of strain 19 and consequent abortion or premature calving. in one of another group of nine pregnant vaccinated with 2.8 x 10(8) viable cells, the ... | 1981 | 6805054 |
| a possible role of cu2+ ions in bovine antibody-antigen interactions. | a preparation of purified bovine igg1 was found to contain 0.5 moles of cu2+ ions per mole of protein. scatchard analysis of the binding of cu2+ ions to bovine igg1 indicated the presence of 4 +/- 1 binding sites with an affinity of 2 x 10(5) m-1. calculations indicate that in bovine plasma, igg1 will not compete significantly with albumin for cu2, whereas a proportion of cu2+ may well be bound to igg1 in colostrum. physiological levels of cu2+ were found to enhance the interaction between igg1 ... | 1982 | 6805057 |
| bovine immunoglobulins and brucellosis. iv. study by microchromatographic technique of class types of persistent antibodies found in heifers vaccinated 12 to 14 months previously with brucella abortus strain 19. | microchromatography with biogel a 1.5 is proposed to distinguish igm and igg antibodies present in biological fluid. the technique allows, with only simple material, a complete separation of the two classes in two hours, with a sample volume of less than 1 ml. the technique, applied to the study of sera from heifers vaccinated 12 to 14 months beforehand with b. abortus strain 19 demonstrated that the persistent antibodies were nearly exclusively of igm type in animals retaining an agglutinating ... | 1981 | 6805409 |
| demonstration that nonspecific bovine brucella abortus agglutinin is edta-labile and not calcium-dependent. | the binding site of b. abortus involved in nonspecific or false-positive agglutination by bovine serum was shown to be ca++-independent in that binding of ca++ by the chelating reagent dp2ta did not decrease titers. the reduction in agglutination by edta, egta, and dpta was thought to be related to a common structural characteristic of these chelating reagents not exhibited by edda and dp2ta or to a lesser extent by dtpa. removal of antigenic entities from the cell by the chelator was excluded a ... | 1982 | 6806363 |
| levamisole potentiation of antigen specific lymphocyte blastogenic response in brucella abortus exposed but nonresponsive cattle. | incubation of brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral blood lymphocytes with b. abortus antigen and levamisole caused a consistently significant increase in [3h] thymidine uptake when compared to cultures without levamisole. levamisole did not potentiate b. abortus-induced blastogenic response of lymphocytes from non-exposed cattle. a dose response study showed that 10 micrograms/culture induced maximum potentiation of b. abortus-induced lymphocyte sti ... | 1981 | 6806973 |
| [effect of temperature and degree of vacuum on the conservation of freeze-dried brucella abortus strain 19 vaccine]. | | 1982 | 6807032 |
| brucella abortus endocarditis: successful treatment of an infected aortic valve. | this report describes a patient with brucella abortus septicemia in whom aortic valvular endocarditis developed. treatment with antibiotics and valve replacement was successful. | 1982 | 6808945 |
| lectin-induced regulation of human thymus-independent anti-tnp responses in vitro. | recently we showed that human hapten-specific t cell-independent (ti) antibody responses could be elicited in vitro with the antigen trinitrophenylated brucella abortus (tnp-ba). although by definition t cells are not required for ti responses, they have also shown capable of regulating such responses in the mouse. in this study we examine the ability of the t cell lectins con a and pha to modulate ti responses of human tonsil cells. addition of the lectins to cultures on day 0 or day 3 resulted ... | 1982 | 6809818 |
| isolated phosphorylcholine binding lymphocytes. i. use of a cleavable crosslinking reagent for solid-phase adsorbent isolation of functional antigen binding cells. | isolation and characterization of balb/c lymphocytes specific for phosphorylcholine is described. the isolation protocol utilizes phosphorylcholine coupled to gelatin coated plates via the cleavable crosslinking reagent n-succinimidyl 3-(2-pyridyldiothio)propionate (spdp). the procedure is rapid, requiring only 1-2 h and conducted entirely at 0-4 degrees c. hapten binding cells are eluted by vigorous pipetting at 4 degrees c with medium containing 20% fetal calf serum. approximately 70% of isola ... | 1982 | 6809832 |
| resistance and susceptibility of mice to bacterial infection. iv. genetic and cellular basis of resistance to chronic infection with brucella abortus. | the number of brucella abortus strain 19 organisms in the spleens of cba/h mice peaked two weeks after intravenous injection of 5 x 10(6) organisms. with the onset of specific cell-mediated immunity, 90% of the bacteria were killed, but approximately 10(6) bacteria persisted up to seven weeks after infection. in contrast, in balb/c, c57bl/10, and b10br mice, bacterial numbers peaked at two weeks but decreased steadily with the onset of bactericidal activity. in all strains, clearance of bacteria ... | 1982 | 6809847 |
| [cell survival of the brucella abortus strain 19 vaccine during the lyophilization process according to the vehicle used]. | ten stabilizing media commonly used by commercial laboratories for the stabilization of brucella abortus strain 19 for lyophilization were studied. six media gave similar results, with viable count averages ranging from 50% to 58%. the standard stabilizer recommended by the world health organization (casitone 2,5%, sucrose 5% and sodium glutamate 1%) was included. the other four media gave viabilities with averages from 19,7% to 38,7%, which represented a highly significant difference. the lowes ... | 1981 | 6810408 |
| serological response of cattle after vaccination and challenge with brucella abortus. | new and currently used serological procedures were evaluated using sera from cattle that were challenged with b. abortus s544 (s544) after vaccination with either b. abortus s19 (s19) or b. abortus 45/20 (s45/20) as calves or adults. in animals vaccinated with s19, titres to the indirect haemolysis test (ihlt) rose more slowly, declined more rapidly and involved fewer animals than did titres to the complement fixation test (cft). in animals vaccinated with s45/20 the rough antigen complement fix ... | 1982 | 6810533 |
| antigens of diagnostic significance in brucella abortus. | | 1982 | 6811117 |
| humoral immune response and expression of spontaneous postnatal amelanosis in dam line chickens. | the delayed amelanotic (dam) chicken line has been developed as a model of human vitiligo. vitiligo in humans and chickens is characterized symptomatically by spontaneous loss of integumentary pigmentation and is often associated with uveitis. in humans, vitiligo is also associated with increased frequencies of several autoantibodies, suggesting a state of immune hyperreactivity. an adult dam chicken population was tested for levels of circulating antibody after challenge with sheep red blood ce ... | 1982 | 6811419 |
| [importance of cross reactions in evaluating the serological diagnosis of human brucellosis. ii. examination of brucellosis patients by the passive hemagglutination reaction using homologous and heterologous erythrocyte diagnostica]. | the study of 270 serum samples obtained from chronic brucellosis patients in the passive hemagglutination test have revealed that in the presence of specific brucellosis hemagglutinins cross reactions with yersinia antigen may occur. this phenomenon is probably due to the presence of common antigenic determinants in the lipopolysaccharide fractions of brucella abortus 99 and yersinia enterocollitica 09. the passive hemagglutination test has revealed that 2-mercaptoethanol-sensitive antibodies (i ... | 1982 | 6812329 |
| effects of nonionic, ionic, and dipolar ionic detergents and edta on the brucella cell envelope. | cell envelopes prepared from smooth and rough strains of brucella were characterized on the basis of lipopolysaccharide and protein content. the action of three kinds of detergents on brucella cell envelopes and escherichia coli control cell envelopes was examined on the basis of the proteins and lipopolysaccharides that were extracted. as compared with those of e. coli, brucella cell envelopes were resistant to nonionic detergents. zwittergents 312 and 316 were most effective in extracting e. c ... | 1982 | 6813315 |
| [development of a splenic infection in mice of 4 strains, inoculated by the venous route, with 3 doses of brucella abortus]. | brucella abortus strain 544 was inoculated at three doses into outbred female swiss mice cd-1 and of1, and inbred strains cba and dba/2. brucella were enumerated in the mice spleens which were first weighed, at 2, 7, 14, 21, 35 and 49 days post-challenge. a phase of multiplication occurred, reaching a maximum between 7 and 21 days according to the mice, but independently of challenge doses. a decreasing phase followed that was still going on in cd-1 and of1 mice at 49 days, but ended by a platea ... | 1982 | 6814335 |
| agglutinins to brucella abortus in horses. | | 1982 | 6814413 |
| antibody levels to brucella abortus, toxoplasma gondii, and leptospira serogroups, in sera collected from healthy people in fiji. | | 1982 | 6816504 |
| study of agglutinins to brucella abortus, b canis and actinobacillus equuli in horses. | horses at a veterinary teaching hospital and a slaughterhouse were surveyed for antibodies to brucella abortus, b canis and actinobacillus equuli. four of the 141 hospitalised horses and none of the 73 slaughtered horses had titres of 1:100 or greater to b abortus. six horses of both populations reacted to the card test. one was culture positive. a card test using b canis antigen was positive in 38 per cent of the sera from hospitalised horses and all of the slaughtered horses. twenty (27.4 per ... | 1982 | 6816581 |
| the reproducibility of results in bovine brucellosis serology and their correlation with the isolation of brucella abortus. | in both the complement fixation test (cft) and the serum agglutination test (sat) titres were reproducible for the most part within a twofold range. they seldom exceeded these limits and never a fourfold range. brucella abortus was successfully isolated in 86% of serologically positive cases and evidence is presented to confirm the use of the 30 international units/ml level in the cft as being diagnostically significant. the sat, when done in microtitration plates, is even more reproducible than ... | 1982 | 6817249 |
| antibodies to brucella abortus in sera from strain 19 vaccinated and non-vaccinated cows as determined by enzyme linked immunosorbent assay and conventional serologic methods. | the sensitivity of an enzyme linked immunosorbent assay was compared with 4 other serologic methods for detecting antibodies in b. abortus vaccinated and non-vaccinated cows. antibodies were detected by elisa in sera from more than 93% of the culture positive cows, however, less than 55% of the infected animals were identified by any other serologic method. similarly, antibodies were detected in 82% of the culture negative cows by the elisa method. | 1982 | 6817509 |
| leptospiral and brucellar titers in frozen bovine sera after 20 years of storage. | generally good agreement was obtained between the rankings of sera titers when originally tested (t1), and those of the same sera 20 years later (t2). at the later time, the majority of samples (88%) were in, or within 1 dilution factor of their classification at t1. test results for brucella abortus and leptospira interrogans var pomona antibodies demonstrated that original titers, in the upper-lower limits of 20 to 50, had decreased over a wide range (20% to 90%), and the later tests show that ... | 1982 | 6817674 |
| isolation of two brucella abortus biotypes from tissues of a naturally infected cow. | brucella abortus biotypes 1 and 2 were recovered from various tissues of a naturally infected cow. this result indicates a need for biotyping multiple isolates of b. abortus from several affected animals in a herd before the biotype of the infecting strain can be definitively established. | 1982 | 6818243 |
| experimental brucella abortus infection in the horse: observations during the three months following inoculation. | five mares, one stallion and a colt foal were inoculated intraconjunctivally with brucella abortus strain 544. no clinical signs of disease developed except mild pyrexia. intermittent bacteraemia was detected in the mares but not in the stallion or foal. antibodies to b abortus became detectable from the second week after inoculation. titres in the serum agglutination and complement fixation tests declined substantially after six to eight weeks but reactions to the coombs antiglobulin, 2-mercapt ... | 1982 | 6818648 |
| a study of cattle infected with brucella abortus and which showed aberrant serological reactions. | sixty cows, 48 of which had been vaccinated with live brucella abortus strain 19 (s19) or with killed b. abortus strain 45/20 (s45/20) and 12 of which were unvaccinated animals, were challenged with b. abortus strain 544. ten of the 27 cattle found to be infected after challenge showed aberrant serological reactions to the rose bengal plate test, serum agglutination test and/or complement fixation test. these 10 cattle were all previously vaccinated with s19 or s45/20. it was concluded that infe ... | 1982 | 6819851 |
| properties of a cell-wall-defective variant of brucella abortus of bovine origin. | the properties of an atypical brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. the organism was gram negative and highly pleomorphic, existing as cocci, coccobacilli, rods, branched and irregular forms which stained with fluorescent antibody conjugates prepared against rough and smooth brucella abortus strains. it produced lecithinase and required at least 15% v/v equine or bovine serum for growth. it did not need supplementary co2 for ... | 1980 | 6820027 |
| [course of chronic brucellosis in mice is not improved by administration of a vaccinal antigen]. | of1 female swiss mice inoculated with brucella abortus virulent strain 544 since four weeks, were treated by subcutaneous injections of a b. abortus 19 cell-wall vaccine extracted by boiling sodium dodecylsulfate suspended in oil adjuvant (pg-b19). vaccinations were administered at 10 or 100 micrograms dose per mouse, two to four times, at 15-30 days interval. groups of six mice were killed at 4 week intervals up to 24 weeks post challenge to follow the evolution of splenic infections by enumera ... | 1982 | 6820248 |
| characteristics of togo strains of brucella abortus from cattle. | thirty togolese strains of brucella from cattle were classified as members of b. abortus biotype 3 by the recommended methods of the subcommittee on taxonomy of this genus. however two unusual characteristics distinguish the togolese strains from the main group of b. abortus: their mean oxidative profile altered on four of the conventional substrates (l-asparagine, l-arabinose, d-galactose and d-xylose) and their very slow growth on usual media. these two original characteristics are discussed f ... | 1982 | 6820250 |
| [enzyme immunoassay to demonstrate antibodies against brucella abortus in the sera of cattle]. | | 1982 | 6820266 |
| on the inactivation of brucella abortus in naturally contaminated milk by commercial pasteurisation procedures. | following concern about the recent publication indicating the possible ability of brucella abortus to survive commercial pasteurisation of naturally contaminated milk, such milk was subjected to biological (bt), serological and bacteriological tests. the raw milk was brucella milk ring test positive and biotype i was isolated from 4/5 bt guinea pigs, the one tested being seropositive to the rose bengal test, and with serum agglutination and complement fixation titres of 160 and 36 international ... | 1982 | 6820666 |
| mother to young transmission of brucella abortus infection in mouse model. | pregnant mice were challenged intraperitoneally with 2 x 10(5) b. abortus strain 544 (weybridge), or with one of its two mutant strains, at day 7 of pregnancy. transmission of brucellosis to infant mice was assessed by enumeration of brucella in spleen, liver and lungs at autopsy. newborns, taken before any suckling were either autopsied or given to foster nurses, either infected or normal. infant mice were autopsied at days 10, 20 and 30. sixty per cent of the newborns were infected at birth. i ... | 1982 | 6821020 |
| [production of coprecipitating antibody in rabbits immunized repeatedly with ovalbumin in different physical states]. | a comparative study has been made of rabbit precipitating and coprecipitating anti-egg albumin antibody in rabbits repeatedly injected with soluble and particulate antigens. four different antigens were used. a) soluble egg albumin. b) polymerized egg albumin. c) egg albumin linked brucella abortus 19 strain. d) brucella abortus 19 strain. rabbits were subcutaneously injected every 2 weeks during 29 weeks. before each inoculation they were bled and the concentration of serum precipitating and co ... | 1982 | 6821520 |
| cross-reactivity of haemophilus somnus antibody in agglutination and complement fixation tests and in the enzyme-linked immunosorbent assay. | the specificity and sensitivity of agglutination, complement fixation, and enzyme-linked immunosorbent assay (elisa) procedures in the detection of antibodies to haemophilus somnus was investigated. h. somnus rabbit immune sera were found to agglutinate pasteurella multocida, staphylococcus aureus, and haemophilus agni and, in some instances, also pasteurella haemolytica, salmonella dublin, streptococcus agalactiae, and corynebacterium pyogenes. in complement fixation tests with saline extracts ... | 1983 | 6841584 |
| comparative efficacy of an experimental s45/20 bacterin and a reduced dose of strain 19 vaccine against bovine brucellosis. | yearling beef heifers were vaccinated with 2 doses of an experimental brucella abortus s45/20 bacterin or a reduced dose of strain 19 (s19) vaccine, or were used as nonvaccinated controls. none of the vaccinated heifers was positive by the acidified plate, rivanol, complement-fixation, or card tests 5 months after inoculation. sixteen of 21 heifers given s45/20 bacterin with trehalose dimycolate adjuvant had delayed hypersensitivity when skin was tested 7 months after initial vaccination, wherea ... | 1983 | 6870000 |
| igg b memory cell subpopulations: differences in susceptibility to stimulation by ti-1 and ti-2 antigens. | spleen cells from mice primed with the thymus dependent (td) antigen trinitrophenyl keyhole limpet hemocyanin several months earlier can be stimulated in vitro to produce an igg anti-hapten response to td as well as thymus independent (ti) forms of the hapten. selective killing of td or ti-2 responding b cells can be accomplished with the corresponding antigen by bromouridine deoxyribose (budr) and light treatment without affecting the other population. in contrast, we show here that selective k ... | 1980 | 6965290 |
| delineation of tolerance-sensitive and tolerance-insensitive b cells in normal and immune defective mice. | b cells responsive to tnp on various thymus-independent carriers acquire immunocompetence at different times during ontogeny and elicit varying levels of responsiveness in immune-defective cba/n mice. such b cells have now been found to display differential tolerance susceptibilities in a hapten-specific in vitro tolerance assay. b lymphocytes from normal neonatal or immune-defective adult cba/n mice responsive to tnp-lipopolysaccharide were shown to be hypersusceptible to tolerogenesis whereas ... | 1980 | 6965299 |
| synergistic genetic defect in b-lymphocyte function. i. defective responses to b-cell stimulants and their genetic basis. | cba/n female mice, which express an x-linked defect in b-lymphocyte function, were mated with c3h/hej male mice, which are unresponsive to lipopolysaccharide (lps). the resulting f1 hybrid females were mated to c3h/hej males. approximately one-half of the backcross (bc.1) males obtained from this mating expressed a more profound immunologic defect than either of the parental strains. spleen cells from these mice were unresponsive to a series of b-cell mitogens including lps prepared from escheri ... | 1980 | 6965307 |
| the development of unusual b-cell functions in the testosterone-propionate-treated chicken. | chickens were treated with 4 mg of testosterone propionate on the twelfth day of embryonic life. bursal remnants of testosterone-treated chickens were very small in size and had very few or no bursal follicles: the lymphoid tissue was replaced substantially by fibrosis. testosterone-treated chickens formed almost exclusively igm antibodies to sheep red blood cells and influenza virus, whereas no igm or igg response to brucella abortus or salmonella pullorum, and no igg response to sheep red bloo ... | 1980 | 6966602 |
| lyb-2 system of mouse b cells. evidence for a role in the generation of antibody-forming cells. | the lyb-2 cell-surface alloantigens of the mouse are selectively and perhaps exclusively expressed in the b lymphocyte lineage, but not on antibody- forming cells. thus if the lyb-2 molecule is concerned in specific b cell function, it must participate in the generative phase of the antibody response. accordingly, monoclonal lyb-2 antibody was found to depress the plaque- forming cell (pfc) response to sheep erythrocytes in 5-d mishell-dutton assays when added within the first 3 d of culture, bu ... | 1981 | 6969779 |
| physiology of igd. i. compensatory phenomena in b lymphocyte activation in mice treated with anti-igd antibodies. | the role of delta-positive cells in the immune response was studied by comparing the effects of treatment with allotype-specific igd hybridoma antibody on homozygous balb/c or sjl/j and heterozygous (balb x sjl)f1 mice. homozygous mice, injected from birth with the relevant anti-delta antibody, made primary or secondary immune responses to intravenously injected trinitrophenyl (tnp)-brucella abortus, tnp-ficoll, and tnp-keyhole limpet hemocyanin, which did not differ significantly from those of ... | 1981 | 6973600 |
| t cell dependent b cell differentiation in the chicken. | transplantation of bursa cells into surgically thymectomized (stx), cyclophosphamide(cy)-treated and x-irradiated (x) newly hatched chicks resulted in a morphological and functional reconstitution of the bursa-dependent immune system only when the transplanted bursa cells were supplemented with thymus cells. without supplementation with thymus cells, morphological restoration of the bursa, antibody formation to sheep red blood cells and brucella abortus, and germinal center formation remained de ... | 1981 | 6974952 |
| alteration of clonal profile. iii. t15 ontogenetic advantages are not sufficient for establishing idiotypic dominance in adoptive transfer. | we have examined the ontogeny of balb/c plaque-forming cell (pfc) responses to phosphorylcholine (pc) from fetal and neonatal liver by using the (cba/n x balb/c)f1 transplantation model. in this system, thymus-dependent (pc-keyhole limpet hemocyanin) and thymus-independent class 1 (pc-brucella abortus, pc-lipopolysaccharide) pc antigens stimulate b cell subpopulations, which functionally emerge early after transfer. responsiveness to a thymus-independent class 2 antigen, c-polysaccharide extract ... | 1981 | 6975348 |
| effect of lipid a incorporation on characterization of liposomal model membranes as thymus-independent type 1 or type 2 immunogens. | we used the following criteria to determine whether liposomal model membranes are thymus-independent type 1 (ti-1) or type 2 (ti-2) immunogens: a) in vivo response of (cba/n x balb/c)f1 male and female mice; b) in vitro response of spleen cells from these mice; c) ontogeny of the response in cultures of (c57bl/6 x dba/2)f1 male cells obtained from mice of different ages; d) ability of a monoclonal anti-delta antibody to block the in vitro response of c57bl/6 spleen cells. the liposomes were sens ... | 1982 | 6976372 |
| polyclonal b cell activation in the acute phase of systemic lupus erythematosus--aspects of pathogenic significance. | b lymphocytes from patients suffering from systemic lupus erythematosus show increasing rates of spontaneous transformation in prolonged cultivation (144 hr). an additional b cell stimulation could be detected following the addition of sera from lupus erythematosus patients (isolated during the acute phase of disease) to the culture medium. in addition enriched mitogenic serum components from lupus sera increase the 3h-thymidine uptake of brucella abortus primed b cells of healthy controls. the ... | 1982 | 6977000 |
| effects of induction of parturition in ewes with dexamethasone or oestrogen on concentrations of immunoglobulins in colostrum, and absorption of immunoglobulins by lambs. | immunoglobulins were measured in colostral whey from untreated ewes or ewes treated on days 142-145 of gestation with 15 mg oestradiol benzoate (odb) or 10, 17.5 or 25 mg dexamethasone m-sulfobenzoate (dex) to induce parturition. dex at all levels reduced the concentrations of immunoglobulins in colostrum, but odb had no effect. in a second experiment, similar treatments of odb and dex were applied on day 143 of gestation. lambs were removed from their dams at birth and fed a standard volume of ... | 1982 | 6982039 |
| antibody formation in mouse bone marrow during secondary type responses to various thymus-independent antigens. | the data presented in this paper show that different thymus-independent (ti) antigens have a differential capacity of inducing antibody formation in mouse bone marrow, both after primary and secondary intravenous immunization. primary immunization with certain ti antigens (e.g., lipopolysaccharide [lps], tnp-lps, dnp-ficoll) induces the appearance of antibody-forming cells not only in the spleen, but also in the bone marrow. a single injection of certain other ti antigens (e.g., pneumococci [pn] ... | 1982 | 6984419 |
| effect of single carcinogenic dose of dimethylnitrosamine on antibody responses in the rat. | the immunodepressive effect of a single, carcinogenic dose of dimethylnitrosamine was tested in a potent model of renal cancer induction using two antigens which differ in their cellular processing. responsiveness to sheep red blood cells (srbc), an antigen requiring the collaboration of both thymus-dependent (t) and thymus-independent (b) lymphocytes was determined by a plaque-forming assay; antibody production to brucella abortus antigen, a thymus-independent antigen was measured in an aggluti ... | 1981 | 7003460 |
| comparison of the enzyme-linked immunosorbent assay with other tests for brucellosis, using sera from experimentally infected heifers. | during an evaluation of h-38 brucella melitensin vaccine, serum samples were collected from 24 hereford-angus heifers (nonvaccinated controls) before exposure and then 11 times between 12 and 102 days after exposure to brucella abortus strain 2308. antibody concentrations were determined by the enzyme-linked immunosorbent assay (elisa) and by standard tube agglutination (sta), 2-mercaptoethanol agglutination (me), microtitration complement-fixation (mcf), and automated complement-fixation (acf) ... | 1980 | 7004278 |
| iga antibodies in the bile of rats. i. some characteristics of the primary response. | about a week after suspensions of sheep red blood cells (srbc) or killed brucella abortus organisms were injected into the peyer's patches of wistar rats specific agglutinins of the iga class appeared in the bile of titres which equalled or exceeded those of the igg and igm agglutinins in the blood serum. the injection of these antigens by conventional routes was relatively ineffective in inducing biliary antibodies. the relationship between the dose of b. abortus injected into the peyer's patch ... | 1982 | 7035341 |
| towards serodiagnosis of serratia marcescens infections: examination of sera from noninfected patients and from experimentally infected rabbits for anti-h and anti-o antibodies; s. marcescens o-antigen cross-reactions with those of other enterobacteriaceae. | sera from 100 patients not infected with serratia marcescens at the time of hospital admission lacked detectable h-immobilizing antibodies against all 20 currently recognized h-antigens of this microorganism. however, various patient sera revealed elevated titers of o-agglutinins against several of the 20 o-antigens of s. marcescens, in a particular o-antigens, o1, o3, o4, o5, o7, o8, o10, o11, o16, o17, o18, o19, and o20. rabbit anti-shigella serogroup b immune serum cross-reacted with s. marce ... | 1981 | 7036590 |
| studies of the immunological activities of the outer membrane protein from escherichia coli. | | 1982 | 7044958 |
| iga antibodies in the bile of rats. ii. evidence for immunological memory in secretory immunity. | the peyer's patches of wistar rats were injected with suspensions of either sheep red blood cells (srbc) or killed brucella abortus organisms in doses that were insufficient to induce the appearance of biliary antibodies. the rats were challenged after periods ranging from 1 week to 1 year with the same dose of antigen given by the same route, and their bile was monitored for the appearance of specific antibodies. the test animals produced biliary antibodies to a much higher titre, and usually m ... | 1982 | 7056564 |
| a comparison of the results of the brucellosis radioimmunoassay and other serological test in experimentally infected cattle. | the serology of 27 heifers found to be positive to culture after inoculation with brucella abortus strain 544, was studied. eighteen heifers had previously been vaccinated with strain 19 or strain 45/20 and nine were unvaccinated. post-infection serum samples were tested for brucella antibodies by radioimmunoassay (ria), complement fixation test (cft), indirect haemolysis test (ihlt) and rose bengal plate test (rbpt). all of the unvaccinated heifers showed strong humoral responses to experimenta ... | 1982 | 7057025 |
| acute and chronic hepatic involvement of brucellosis. | two patients with brucellosis are presented; one with acute hepatitis and the other with a chronic draining sinus at the right costal margin which originated from an abscess of the liver. brucella abortus infection may be manifest by nonspecific hepatic inflammation or, occasionally, by granulomatous hepatitis. rarely, cirrhosis may result if untreated. chronic hepatic and splenic involvement, which is characterized by tissue calcification, can be seen in brucella suis infection. suppurative com ... | 1982 | 7084621 |
| [enteral yersiniosis--a serious disease? current knowledge of clinical aspects, diagnosis and therapy]. | enteral yersiniosis is caused either by y. enterocolitica 0-group i (syn. serotype 0:3) and 0-group v (syn. serotype 0:9) or y. pseudotuberculosis type i-vi. the clinical symptoms are mostly like enteritis, enterocolitis, acute abdomen, mesenteric lymphadenitis, or ileitis terminalis. post-infection reactions are possible like septicemia, arthritis and erythema nodosum. only cultural and serological examinations confirm the diagnosis of enteral yersiniosis. in the judgement of serological result ... | 1982 | 7095694 |
| unilateral orchitis in a bull caused by brucella abortus biotype1. | a case of brucella orchitis in a brahman bull is described. positive serological titres were detected 1 month before clinical symptoms became obvious, whereas the seminal plasma only contained detectable levels of antibody after the onset of orchitis. it is concluded that serological assays on the seminal plasma alone are inadequate to detect sub-clinically infected animals, and consequently particularly bulls that are intended for use in ai centres should be subjected to a full battery of serol ... | 1982 | 7097709 |
| the influence of site of antigen deposition on the local immune response in the mammary gland of the ewe. | experiments were carried out to compare the local antibody responses in mammary glands of ewes immunized by infusion of antigen (killed brucella abortus) into the lactiferous sinuses (trans-epithelial presentation of antigen) or injection into the mammary tissue near the supramammary lymph node (interstitial presentation of antigen). although both methods of antigen presentation resulted in similar antibody levels in blood, infusion of antigen into the lactiferous sinus resulted in significantly ... | 1982 | 7121316 |
| reduction of brucella species and francisella tularensis cross-reacting agglutinins by dithiothreitol. | cross-reaction agglutinin titers to brucella abortus antigen were found in 42 of 128 tularemia serum specimens, and cross-reaction titers to francisella tularensis antigen were found in 8 of 34 brucellosis serum specimens. the cross-reaction titers were reduced to 10 or less by dithiothreitol, suggesting that the titers are due to immunoglobulin m antibody. | 1982 | 7153327 |
| latent bovine brucellosis. | adult cattle in a herd suffering from widespread infection with brucella abortus biotype 2 were slaughtered during the eradication campaign. a heifer calf fom that herd was moved to a fresh herd which remained apparently free from brucellosis until nine years later when the same animal produced a strongly positive serological reaction and brucella abortus biotype 2 was isolated from its milk. | 1982 | 7157620 |
| [barbital buffer in tablets as a solvent for complement fixation test (cft)]. | the purpose of this paper was to develop a tableting technology of barbital buffer as a standard diluent easy in use for complement fixation test (cft). as starting point, barbital (veronal) buffer after alton et al. [1] was assumed. among 4 solubilizers polyvinyl pyrrolidone (pvp 10 000) was chosen, which provided a good solubility of the barbital in distilled water at 20 degrees c and it affected favourably the complement activity (c'). pvp 10 000 added to the components of barbital buffer at ... | 1982 | 7167394 |
| [development of brucella abortus, brucella melitensis and brucella and brucella suis infection infection in mice to a chronic stage and cure]. | | 1982 | 7168538 |
| latent carriers of brucellosis. | of 272 heifers subjected to a brucellosis anamnestic test following one injection of k45/20a vaccine, 53 (19%) gave a positive result to the anti-bovine globulin test and 21 (8%) gave a complement fixation test titre at 1/16 or more. three heifers (1%) gave a positive serum agglutination test (sat) titre (60 iu or more) 12 weeks after the second injection of k45/20a vaccine. one of the three aborted at seven months' gestation (brucella abortus culture positive), another had an sat level of 100 i ... | 1980 | 7189074 |
| antibody-containing cell response of sheep to intestinal infusion of killed brucella abortus cells. | | 1980 | 7190880 |
| studies on in vitro lymphocyte stimulation assay in cattle naturally infected with brucella abortus and in cattle vaccinated with strain 19. | cell-mediated immune responses of cattle in infected herds vaccinated with brucella abortus, strain 19, and of nonexposed controls were studied by an in vitro lymphocyte stimulation test (lst). brucella abortus soluble antigen was used as a specific stimulator of lymphocytes. lymphocyte suspensions were prepared from peripheral blood of cattle by the ficoll-diatrizoate technique. cultures were labeled with [3h]thymidine and were assayed for [3h]thymidine incorporation into dna by liquid scintill ... | 1980 | 7224283 |
| effect of bursal perfusate on igg antibody production in incomplete surgically and hormonally bursectomised chickens. | bursa of fabricius tissues of untreated four-week-old and of cyclophosphamide-treated 10-day-old chickens were cultured by a perfusion method and the perfusate used in a study on the enhancement of antibody production and conversion in chickens. the bursal perfusates were given to incompletely immunosuppressed chickens (surgical bursectomy or hormonal bursectomy) and to control chickens which were immunised with either sheep red blood cells or brucella abortus. the results indicated that bursal ... | 1981 | 7313319 |
| outbreak of brucellosis at a south-australian abattoir. 2. epidemiological investigations. | the outbreak of human brucellosis among employees of a large south australian abattoir described previously coincided with an increase in the number of cattle showing a positive serological reaction for brucella abortus being slaughtered. comparisons showed that two other abattoirs in the area were slaughtering larger numbers of such cattle, but no cases of human brucellosis were diagnosed there. this suggested an additional risk at the abattoir concerned. all infected men had been employed in a ... | 1981 | 7334991 |
| vaccination of heifers with a reduced dose of brucella abortus strain 19 vaccine before first mating. | ten jersey heifers aged 14 to 23 months were vaccinated with 2.25 x 10(8) cells of living brucella abortus strain 19 vaccine. they and 10 similar non-vaccinated heifers were subsequently mated and when about 6 months pregnant were challenged by the conjunctival application of a virulent culture of b. abortus. the serological responses to vaccination was much less than is usually seen following vaccination with the normal dose of strain 19, especially when the indirect haemolysis test was used. a ... | 1981 | 7340776 |
| cross-reaction to legionella pneumophila antigen in sera with elevated titers to pseudomonas pseudomallei. | a significantly greater incidence (p less than 0.005) of legionella pneumophila microagglutination titers greater than or equal to 32 was found in sera with elevated titers to pseudomonas pseudomallei as compared with sera with negative titers for p. pseudomallei antibodies. the greater incidence of l. pneumophila titers in these sera suggests that l. pneumophila and p. pseudomallei share an antigen. the incidence of l. pneumophila microagglutination titers of greater than or equal to 32 in sera ... | 1980 | 7354127 |
| brucellosis in moose (alces alces). a serological survey in an open range cattle area of north central british columbia recently infected with bovine brucellosis. | a serological survey for brucella abortus antibodies in mature cow moose (alces alces) was made in an area of northcentral british columbia which recently had been heavily infected with bovine brucellosis and in which there was considerable intermixing of moose and range cattle. no evidence of brucella infection was found in the moose tested and it was concluded that they were probably not of great significance in the epidemiology of bovine brucellosis in the study area and were therefore unlike ... | 1980 | 7363258 |
| abortion in sheep near kano, nigeria. | a sporadic natural infection of sheep with brucella abortus in a sheep breeding centre in kano state, nigeria, is reported. sera and milk samples from the flock of sudanese long- and fat-tailed sheep used for cross-breeding purposes were examined for brucellosis by the rose bengal plate test (rbpt), serum agglutination test (sat) and milk ring test (mrt) respectively. the overall positive reactor rate to sat was 14.5%. all 22 ewe milk samples examined by mrt were positve and brucella abortus was ... | 1980 | 7368315 |
| the effects of a megalevel of vitamin c on the immune response of the chicken. | birds fed diets supplemented with 1% vitamin c experienced significant increases in serum levels of vitamin c. cell-mediated immunity and antibody response to sheep red blood cells, a t-cell dependent antigen, were not influenced by feeding megalevels of vitamin c. however, the antibody response to a t-independent antigen, brucella abortus, was lower in neonates and higher in adults in the presence of increased levels of vitamin c. | 1980 | 7402997 |
| the microplate agglutination test: a simple technique to assist in the differentiation of bovine brucellosis and yersiniosis. | triphenyltetrazolium chloride stained brucella abortus 0 and yersinia enterocolitica 09 0 and 0h antigens were used in the microplate agglutination test. it was shown that the results of these tests could be used to differentiate anti-brucella antibodies from antibodies induced by motile y enterocolitica 09 in cattle sera. | 1980 | 7434502 |
| thymic abnormalities and growth hormone deficiency in dogs. | a high frequency of occurrence of a wasting disease, unthriftiness, and retarded growth was observed in a group of inbred weimaraner dogs. affected pups had a small thymus gland, with a marked absence of thymic cortex. a litter of eight pups from a sire and dam that were known to have produced affected offspring was chosen for further study. the pups had normal concentrations of wbc and gamma-globulins and were able to produce antibody in response to brucella abortus. two pups in the litter deve ... | 1980 | 7447121 |
| [serodiagnosis of human yersinia infections. preliminary results]. | preliminary data are reported on experience with newly developed complement-fixing (cf) antigens prepared from cultures of type 3 and type 9 yersinia enterocolitica and from yersinia pseudotuberculosis. antigens were immunologically potent and not anti-complementary, thus making it possible to obtain satisfactory serologic tests. the results may be summarized as follows: 1. yersinia cf antigens do not react with sera from healthy blood donors. there was only one low titer reaction with type 9 an ... | 1980 | 7455648 |
| the indirect haemolysis test (ihlt) for bovine brucellosis - comparisons with the complement fixation test (cft) in vaccinated and experimentally infected cattle. | cattle were vaccinated with brucella abortus strain 19 subcutaneously in doses ranging from the normal (2.0 ml) dose of standard vaccine down to 1/400 of the normal dose, and via the conjunctival sac with 1/2 of 1/20 of the normal dose. under all vaccination regimes serum antibody titres in the complement fixation test (cft) rose more rapidly, reached higher levels, declined more slowly and involved a greater proportion of animals, than titres in the indirect haemolysis test (ihlt). the interval ... | 1980 | 7458780 |
| [fever, hepatic granulomatosis, electrocardiogram changes, and a positive serodiagnosis for yersinia enterocolitica: two case reports (author's transl)]. | though no bacteria were isolated, a diagnosis of yersinia enterocolitica infection was confirmed in two patients by strongly positive serology tests, with levels much above the normal. both patients had fever, hepatic granulomatosis, and electrocardiogram anomalies, and there was associated dysuria and prostatitis in one case. the authors review the hepatic, urinary, and electrocardiogram manifestations reported in the published literature, and describe the antigenic and serologic relationships ... | 1980 | 7469239 |
| production of brucella allergens and evaluation of their biological activity in a guinea-pig bio-assay. | a study was conducted to evaluate the biological activity of brucella allergens extracted with hydrochloride or trichloroacetic acid. smooth and mucoid brucella abortus cells and the medium in which brucellae were propagated were used to prepare the allergens. the biological activity of the allergens was estimated in guinea-pigs sensitized with brucella abortus strain 544. the guinea-pigs were intradermally injected with several allergen dilutions. the dilutions were coded and randomized for sit ... | 1995 | 7483897 |
| restriction site polymorphism of the genes encoding the major 25 kda and 36 kda outer-membrane proteins of brucella. | seventy-seven brucella reference and field strains from different geographic origins and hosts representing the six recognized species and their different biovars were analysed for diversity of their genes encoding the major 25 and 36 kda outer-membrane proteins (omps) by pcr-rflp. the 25 kda omp is encoded by a single gene (omp25) whereas two closely related genes (omp2a and omp2b) encode and potentially express the 36 kda omp. analysis of pcr products of the omp25 gene digested with nine restr ... | 1995 | 7496522 |
| immunochemical identification of brucella abortus lipopolysaccharide epitopes. | sera from brucella abortus-infected and -vaccinated bovines recognized four lipopolysaccharide (lps) determinants: two in the o-polysaccharide (a and c), one in the core oligosaccharide from rough brucella lps (r), and one in lipid a (la). from 46 different hybridomas secreting monoclonal antibodies (mabs) against various lps moieties, 9 different specificities were identified. two epitopes, a and c/y, were present in the o-polysaccharide. two epitopes were found in the core oligosaccharide (r1 ... | 1994 | 7496947 |
| improved competitive enzyme immunoassay for the diagnosis of bovine brucellosis. | a modification of the competitive enzyme-linked immunosorbent assay (c-elisa) for differentiating the antibody response of cattle vaccinated with brucella abortus strain 19 and b. abortus infected cattle is described. this assay utilizes lipopolysaccharide as the antigen, immobilized on a polystyrene matrix, and a monoclonal antibody (m84) with specificity for an epitope of the o-polysaccharide. a goat anti-mouse igg antibody-enzyme conjugate is used for detection. the specificity of the modifie ... | 1995 | 7502488 |
| antibody response to the 89-kda outer membrane protein of brucella in bovine brucellosis. | the antibody response of cattle to the minor 89-kda outer-membrane protein (omp) of brucella was measured by indirect elisa with the purified protein and compared with the antibody response to smooth lipopolysaccharide (s-lps). pre-incubating sera with sonicated cell extracts of escherichia coli prevented the binding of antibodies from uninfected animals to the 89-kda omp, suggesting the presence of one or more cross-reactive epitopes on this protein. in cattle infected experimentally with bruce ... | 1993 | 7504112 |
| lymphocyte proliferation in response to immunodominant antigens of brucella abortus 2308 and rb51 in strain 2308-infected cattle. | lymphocyte proliferation in response to proteins from the brucella abortus strain 2308 (s2308) and the lipopolysaccharide (lps) o-antigen-deficient mutant of s2308, strain rb51 (srb51), was measured in s2308-infected cattle following abortion. supramammary and superficial cervical lymph node lymphocytes from infected cattle proliferated most when incubated with 27- to 18-kda proteins of s2308 or srb51. proteins of srb51, which contained no lps o antigens, induced lymphocyte proliferation similar ... | 1994 | 7523295 |
| improvements to the competitive elisa for detection of antibodies to brucella abortus in cattle sera. | this paper reports improvements in the competitive elisa (celisa) for the detection of serum antibodies to brucella abortus through modification of the coating of antigen and of the enzyme labelling of the monoclonal antibody conjugate. the covalent linkage of poly-l-lysine to the o-polysaccharide antigen of brucella abortus enhanced its binding to the polystyrene matrix which resulted in a more efficient and reliable celisa. optimal discrimination between vaccinated and infected cattle was achi ... | 1994 | 7523456 |
| modulation of immune responses in balb/c mice vaccinated with brucella abortus cu-zn superoxide dismutase synthetic peptide vaccine. | three peptides, peptide 1 (ggdnysdkpeplgg), peptide 2 (laeikqrslmvhgg) and peptide 3 (ggapgekdgkivpag), were synthesized based on the amino acid sequence of brucella abortus cu-zn superoxide dismutase. these peptides were selected on the basis of their predicted hydrophilicity, flexibility and antigenicity profiles. the three peptides, singly or in combination, with or without the adjuvant monophosphoryl lipid a were administered to balb/c mice as vaccines for brucellosis. the protective and imm ... | 1994 | 7526568 |
| expression of tfx and sensitivity to the rhizobial peptide antibiotic trifolitoxin in a taxonomically distinct group of alpha-proteobacteria including the animal pathogen brucella abortus. | three phylogenetically distinct groups within the alpha-proteobacteria which differ in trifolitoxin sensitivity are described. trifolitoxin sensitivity was found in strains of agrobacterium, brucella, mycoplana, ochrobactrum, phyllobacterium, rhodobacter, rhodopseudomonas, rhodospirillum, and rhizobium. strains of agrobacterium, brucella, phyllobacterium, rhizobium, and rhodospirillum were capable of producing trifolitoxin upon conjugal transfer of tfxabcdefg. | 1994 | 7527627 |
| specific detection of brucella dna by pcr. | a pcr assay with primers derived from the 16s rrna sequence of brucella abortus was developed. nine different combinations between six primers were tested. one pair of primers, which amplified a 905-bp fragment, was selected. as little as 80 fg of brucella dna was detected by this method. dnas from all of the representative strains of the species and biovars of brucella and from 23 different brucella isolates were analyzed and yielded exclusively the 905-bp fragment. no amplification was detecte ... | 1995 | 7538508 |
| isolation and characterization of ropa homologous genes from rhizobium leguminosarum biovars viciae and trifolii. | ropa encodes a 36-kda outer membrane protein of rhizobium leguminosarum bv. viciae strain 248 which constitutes the low-m(r) part of antigen group iii (r.a. de maagd, i.h.m. mulders, h.c.j. canter cremers, b.j.j. lugtenberg, j. bacteriol. 174:214-221, 1992). we observed that genes homologous to ropa are present in strain 248 as well as in other r. leguminosarum strains, and we describe the cloning and characterization of two of these genes. sequencing of a 2.2-kb bg/ii fragment from r. leguminos ... | 1995 | 7545151 |
| structural characterization of the active site of brucella abortus cu-zn superoxide dismutase: a 15n and 1h nmr investigation. | prokaryotic cu-zn superoxide dismutases (sods) are rare and poorly characterized compared to their eukaryotic counterparts. to better characterize the structure of the prokaryotic enzyme, an nmr investigation of brucella abortus cu-zn sod in the reduced form was undertaken. the enzyme studied was a recombinant form, expressed in escherichia coli. the enzyme initially lacked a full complement of cu and zn ion. after demetallation and remetallation with a stoichiometric amount of cu and zn ion, th ... | 1995 | 7547969 |
| lack of a role for natural killer cells in early control of brucella abortus 2308 infections in mice. | studies were conducted to determine if natural killer (nk) cells are important for early control of the virulent strain brucella abortus 2308 following infection of mice with high or low challenge doses. splenocytes from c57bl/10 and balb/c mice that had been infected with the lower dose of b. abortus displayed increased cytotoxicity against yac-1 cells during the first week after infection, while infection of c57bl/10 mice with the higher challenge dose either did not alter the level of nk cyto ... | 1995 | 7558315 |
| [use of the b19 strain in the prevention of bovine brucellosis in northern cameroon. study of effect of the dose on seroconversion rate and duration in female zebu]. | four herds of zebus from northern cameroon totalling 136 animals were vaccinated subcutaneously with the following doses of brucella abortus strain 19: 5 x 10(9) colony-forming units (cfu), 10(9) cfu, 5 x 10(8) cfu and 10(7) cfu. twenty-eight days after vaccination, the following seroconversion rates were observed respectively: 97.4, 96.2 84.2 and 73.3%. of the 52 animals which could be tested subsequently including 39 over one year old on the vaccination day, only one showed antibodies 6 months ... | 1995 | 7569229 |
| selective humoral immune response of balb/c mice to brucella abortus proteins expressed by vaccinia virus recombinants. | genes encoding brucella abortus cu/zn superoxide dismutase (sod) and a 54 kda escherichia coli htra homologue were cloned into shuttle plasmids puv-1 and psc11, and transfected into vaccinia virus to develop recombinants vubsod and vsb54. control vaccinia virus recombinants vuv-1 and vsc11, carrying only the beta-gal reporter gene but no b. abortus dna were also developed. recombinants were analyzed in western blotting using a polyclonal b. abortus immune serum. vubsod expressed a protein of app ... | 1995 | 7571368 |
| evaluation of a potassium chloride extract of brucella abortus in an elisa for detecting brucella antibodies in bulk tank milk samples from cows. | an enzyme-linked immunosorbent assay (elisa) was developed using as antigen a potassium chloride extract of brucella abortus strain 1119-3 for detecting brucella antibodies in bulk tank samples of cow's milk. three-hundred-thirty-four milk ring test (mrt) suspicions milk samples originating from cattle herds in 13 states and 106 brt negative milk samples were analyzed. fifty-four of 334 mrt suspicious milk samples were positive on elisa; bacteriologic examinations revealed b. abortus field strai ... | 1995 | 7571369 |
| cd8+ type 1 cd44hi cd45 rblo t lymphocytes control intracellular brucella abortus infection as demonstrated in major histocompatibility complex class i- and class ii-deficient mice. | genetically engineered mice with a targeted disruption in the beta 2-microglobulin (beta 2-m) gene or the h2-i-a beta chain (a beta) which lack functional cd8+ or cd4+ t cells, respectively, were used to assess the role of t cell subsets in brucella abortus infection. murine brucellosis was markedly exacerbated in beta 2-m-deficient mice (beta 2-m-/-) compared to a beta mutant (a beta-/-) or c57bl/6 mice, strongly indicating that optimal resistance to b. abortus requires cd8+ t cells. splenocyte ... | 1995 | 7589125 |
| cloning and characterization of the glucokinase gene of brucella abortus 19 and identification of three other genes. | a clone from brucella abortus 19 complemented an escherichia coli strain deficient in phosphorylation of glucose. open reading frames similar to e. coli mepa, glk, and genes encoding atp-coupled exporters were found in the sequence. a fourth affected growth on minimal media of the ptsi glk strain with various carbon sources. | 1995 | 7592399 |
| persistence of brucella abortus in the livers of t cell-deficient nude mice. | brucella abortus is sequestered in lymphoid tissues, bone, and liver during chronic asymptomatic brucellosis of cattle and humans. the sites of cellular infection, cytopathology of infected cells, and mechanisms of bacterial recrudescence have not been identified. a laboratory model is needed for the study of chronic brucellosis. | 1995 | 7603046 |