| oncogenic viruses and cervical cancer. | | 1989 | 2564994 |
| epstein-barr virus silver anniversary. | | 1989 | 2566738 |
| epstein-barr virus/complement receptor and epithelial cells. | | 1989 | 2567860 |
| viral genomes in lymphomas of patients with sjögren's syndrome. | the recent isolation of a new member of the herpes virus family (human herpes virus-6, hhv-6) from patients with lymphoproliferative diseases prompted us to examine biopsies from six patients with primary sjögren's syndrome (ss) who developed non-hodgkin's lymphoma. five ss patients developed b-cell lymphoma and one developed a t-cell lymphoma based on immunoglobulin and t-cell antigen receptor (tcar) gene rearrangements. in two ss patients with b-cell lymphomas, viral dnas were detected, includ ... | 1989 | 2571338 |
| antibodies to epstein-barr virus-induced early antigens in blood donors. | igg class antibodies to the early antigen complex (ea; d + r components) of the epstein-barr virus (ebv) were found by indirect immunofluorescence in titres greater than or equal to 1:10 in 196 (49.4%) out of 397 blood donors and titres greater than or equal to 1:20 in 84 (21.2%) of these subjects. anti-ea titres of greater than or equal to 1:2560 were detected in 6 sera, anti-ea(d) only in 17 donors (4.3%). additional ebv-specific antibody tests were performed in sera with anti-ea-igg titres of ... | 1989 | 2574941 |
| persisting illness and fatigue in adults with evidence of epstein-barr virus infection. | clinical, serologic, virologic, and immunologic evaluations for 31 adults with chronic illness and fatigue suggested that 23 had persisting epstein-barr virus infection. among these 23 patients, cellular immune mechanisms were generally normal, but 4 had mild immunoglobulin deficiencies. however, 20 patients had abnormal serologic profiles specific for epstein-barr virus shown by significantly elevated titers of antibodies to the viral capsid antigen or early antigen, or by a deficiency of late- ... | 1985 | 2578268 |
| large-scale purification of epstein-barr virus membrane antigen gp340 with a monoclonal antibody immunoabsorbent. | a purification method has been elaborated to isolate epstein-barr (eb) virus membrane antigen, gp340, in milligram amounts. the gp340 was prepared from detergent extracts of b95-8 cells by affinity chromatography with a monoclonal antibody immunoabsorbent. bound material was eluted and the eluate, consisting of 50% gp340, was then fractionated by gel filtration. the final gp340 product was antigenically active and 95% pure. the purification method was found to be rapid and reproducible with no l ... | 1985 | 2579161 |
| human monoclonal antibodies produced by epstein-barr virus transformed cell lines bind protein a. | epstein-barr virus (ebv) is a polyclonal t-independent activator of viral receptor positive human b lymphocytes. lymphocytes infected in vitro with the virus are transformed into immortalized cell lines [nilsson, k, and klein, g. (1982) adv. cancer res. 37, 319]. in this way human cell lines that secrete specific igm, igg and iga monoclonal antibodies are established. protein a is also a polyclonal t-independent b cell activator [langone, j. j. (1982) adv. immunol. 32, 157], the targets of which ... | 1985 | 2580779 |
| genetic origin of human anti-insulin antibodies. | numerous studies have characterized the specificity of human anti-insulin antibodies; however, little is known about their genetic origin. to initiate molecular studies, b cells that bind human insulin were selected from the peripheral blood of diabetic donors and transformed with epstein-barr virus. the resulting anti-insulin b-cell lines were cultured at limiting dilution and examined for immunoglobulin heavy-chain gene rearrangements on southern gels. these studies demonstrated the clonality ... | 1989 | 2584073 |
| b-cell chronic lymphocytic leukaemia: clonal chromosome abnormalities and prognosis in 89 cases. | the results of cytogenetic studies are reported in 89 patients with b-cell cll. lps (e. coli lipopolysaccharide), pwm (pokeweed mitogen), pha (phytohaemagglutinin), ebv (epstein-barr virus), tpa (phorbol 12-myristate 13-acetate), and la (leucoagglutinin) were used as mitogens. mitoses were obtained from 78 cases. clonal aberrations could be demonstrated in 26 cases. trisomy 12 was the most frequent finding (8 cases) and was sole abnormality in 4 cases. chromosomes #14, #17, and #11 were involved ... | 1989 | 2612612 |
| influences of related retroviruses on lymphocyte functions. | the human immunodeficiency virus (hiv-1) is known to be profoundly immunosuppressive [spickett and dalgleish (1988) clin. exp. immunol. 71, 1]. in this communication, we have studied the influences of hiv-1 (bh10), hiv-2 (lav-2) and stlv-3 on b and t cells from healthy volunteers. b lymphocytes were found to differentiate into immunoglobulin secreting cells in response to stimulation by proteins of hiv-1 and lav-2, but not by stlv-3. this response was obtained at protein concentrations of 0.05-0 ... | 1989 | 2631867 |
| discrimination of antibodies against antigens of different mhc loci in human sera by monoclonal antibody-specific immobilization of leukocyte antigens. | to detect human antibodies against antigens of different major histocompatibility complex loci, particularly of class ii specificity, a newly developed enzyme immunoassay for platelet antibodies was adapted for the use of lymphocytes as target cells. peripheral blood lymphocytes, phytohemagglutinin-stimulated t cells, or epstein-barr virus-transformed b cells were simultaneously incubated with a monomorphic class- or locus-specific monoclonal antibody and the human antibody to be investigated. a ... | 1989 | 2661505 |
| perspectives on research and diseases of the tropics: an asian view. | diarrhea, pneumonia, and malnutrition account for most of mortality and morbidity in children in developing countries. the expanded program of immunization (epi) is making progress with more than 50% of children under the age of 1 year receiving vaccination against the 6 epi-listed diseases. the eradication of poliomyelitis by 2000 is realistic, so that the world could be smallpox- and polio-free by the 21st century. in july-august 1988 a cholera epidemic erupted in delhi, india in which sever ... | 1989 | 2698093 |
| retroviral-mediated transfer and expression of hepatitis b e antigen in human primary skin fibroblasts and epstein-barr virus-transformed b lymphocytes. | previously, an amphotropic retroviral expression system coding for the neomycin resistance gene was developed and used to synthesize hepatitis b e antigen (hbeag) and hepatitis b core/e antigen (hbc/eag) in transfected mouse nih 3t3 fibroblasts (a. mclachlan et al., 1987, j. virol. 61, 683-692). in the present study, these transfected cell lines were infected with a helper amphotropic murine leukemia virus resulting in the production of infectious recombinant retrovirus. the recombinant retrovir ... | 1989 | 2783354 |
| identification of murine complement receptor type 2. | a rabbit antiserum reactive with the human complement component c3d/epstein-barr virus receptor (complement receptor type 2, cr2) immunoprecipitates a mr 155,000 murine b-cell surface antigen. the apparent molecular weight and cellular distribution of this murine antigen are similar to those of human cr2. cells expressing the murine protein bind sheep erythrocytes coated with antibody and murine c1-c3d but do not bind epstein-barr virus at all. the monospecific antiserum to human cr2 together wi ... | 1989 | 2783485 |
| biphenotypic leukemic lymphocyte precursors in cd2+cd19+ acute lymphoblastic leukemia and their putative normal counterparts in human fetal hematopoietic tissues. | during detailed immunophenotypic analyses of marrow blasts from 336 acute lymphoblastic leukemia (all) patients, a very small percentage of cases reactive with b-cell-directed as well as t-cell-directed monoclonal antibodies (moabs) were identified. five all cases were biphenotypic since they coexpressed cd2 (tp50) and cd19 (bp95) antigens at the single-cell level. the composite immunophenotype of these biphenotypic all cases was [tdt+hla-abc+cd2+cd3-cd10+cd13-cd14-cd16-cd19+cd20+ ++-cd21-cd33-c ... | 1989 | 2784064 |
| studies on the role of cd43 in human b-cell activation and differentiation. | the monoclonal antibody (moab) b1b6 to human leucocyte sialoglycoprotein, cd43, induces aggregation of t cells and delivers progression signals early during activation of both t and b cells in the presence of primary activators of protein kinase c. in this report we further studied the role of cd43 in human b-cell activation and differentiation. about 5-10% of resting tonsillar b cells are cd43+. in the presence of tpa or antibodies to cdw40, the proportions of cd43+ cells drastically increased. ... | 1989 | 2785706 |
| in vitro synthesis of human immunodeficiency virus-specific antibodies in peripheral blood lymphocytes of infants. | an assay system was developed for the analysis of antibodies secreted in vitro against human immunodeficiency virus (hiv) by cultured peripheral blood lymphocytes of hiv-infected individuals. cultures of peripheral blood lymphocytes were established with medium alone or with medium containing epstein-barr virus (ebv) or pokeweed mitogen. hiv antibodies were determined by an elisa performed with commercial kits in which a whole virus extract served as antigen. optimal antibody secretion was detec ... | 1989 | 2798424 |
| predominance of mex+ cells in newly-established human lymphoblastoid cell lines. | previous studies have demonstrated that approximately one-third of human lymphoblastoid cell lines (lcls) are deficient in removing o6-methylguanine residues because of the lack of o6-alkylguanine-dna alkyltransferase (o6-agt) activity. such lcls have been designated mex-, while the proficient lcls are mex+. our determinations of o6-agt activity as a function of cellular protein concentration on 37 previously-established lcls disclosed that the expression of the enzyme was high in 14 (mex+) and ... | 1989 | 2805228 |
| reconstitution of cytotoxic t lymphocyte activity following allogeneic bone marrow transplantation in man. | longitudinal studies over an eight-month period have been performed to follow the t killer response restoration after allogeneic bone marrow transplantation (bmt). the capacity of the patient's peripheral blood mononuclear cells (pbm) to develop cytotoxic effector cells directed either against allogeneic cells or against epstein-barr virus (ebv) or herpes-simplex virus-1 (hsv-1)-infected syngeneic cells was tested monthly. the data suggest that in most cases the cytotoxic t lymphocyte (ctl) acti ... | 1987 | 2820090 |
| identification of an epstein-barr virus early gene encoding a second component of the restricted early antigen complex. | when the latent epstein-barr virus (ebv) genome in b95-8 cells is induced into a replicative phase, two abundant early rnas are transcribed rightward from the ebv bamhi h dna fragment into bamhi f. analysis of cdna clones prepared from the rna of cells replicating ebv revealed that both rnas contain the bhrf1 open reading frame. part of bhrf1, cloned into a prokaryotic fusion protein expression vector, expressed a fusion protein in escherichia coli and the purified fusion protein was used to gen ... | 1987 | 2820125 |
| [inhibition and reversion of epstein-barr virus dna replication in the h18 cell line]. | | 1987 | 2820170 |
| autocrine growth function of interleukin-1-like molecules secreted by neoplastic human b cells. | | 1987 | 2820208 |
| demonstration of epstein-barr virus in immunoblastic sarcoma of b-cells arising in a child with primary immunodeficiency disease. | the subject of this investigation was an 11-month-old infant girl who presented with a pathological fracture of the right femur due to a metastasis from an abdominal immunoblastic sarcoma. her past history included recurrent, intractable bacterial and fungal infections. investigations of her immune status revealed low numbers of t-lymphocytes, a reversed t-helper (th)/t-suppressor (ts) cell ratio, no response of her peripheral blood lymphocytes to pokeweed mitogen, phytohemagglutinin, concanaval ... | 1987 | 2820254 |
| affective disorder and viral infections. | | 1987 | 2820339 |
| virus-associated hemophagocytic syndrome: identification of an immunoproliferative precursor lesion. | virus-associated hemophagocytic syndrome (vahs) is a nonneoplastic, generalized histiocytic proliferation with marked hemophagocytosis associated with a systemic viral infection. histologic studies of lymph nodes usually show lymphoid depletion and histiocytic proliferation. in this report we describe the case of a patient with epstein-barr virus-associated vahs in which initial lymph node biopsy samples showed an immunoproliferative lesion that preceded the usual generalized histiocytic prolife ... | 1987 | 2820860 |
| salivary gland cancer in alaskan natives, 1966-1980. | | 1987 | 2820863 |
| epstein-barr virus-related syndromes: implications for headache research. | | 1987 | 2820897 |
| strategies to analyse mutagenesis in mammalian cells using simian virus 40 or shuttle vectors. | the use of exogeneous dna probes, which replicate extrachromosomally, is proposed in order to study spontaneous and induced mutagenesis in mammalian cells. simian virus 40 has already proved to be very useful, since it has provided much important information in this field. recently, several shuttle vectors have been designed for this purpose; however, it seems that these molecules have high spontaneous mutation frequencies when replicating in mammalian cells. we have developed new alternative sy ... | 1987 | 2821023 |
| hla class i antigens on normal and leukemic cells (quantitative analysis). | a monomorphic anti-class i monoclonal antibody, st01, found in our laboratory, was used to quantify class i antigens on normal and leukemic cells, using a "celisa" technique. saturation graphs were used to compare the quantity of class i antigens on normal pbl (25 cases) with that on the following types of leukemic cells: a) common acute lymphoblastic leukemias (call) (11 cases), b) mature b lymphocytic proliferations (16 cases), c) t hemopathies (9 cases), d) non-lymphoid leukemias (9 cases). i ... | 1987 | 2821653 |
| electron microscopy of binding of epstein-barr virus (ebv) nuclear antigen (ebna-1) to ebv dna. | specific binding sites for epstein-barr virus (ebv) nuclear antigen (ebna-1), isolated and semipurified from ebv-transformed nonproductive raji cells, were visualized on the molecule of ebv dna by electron microscopy and mapped. two measures had to be applied to counteract the limited purity of the ebna-1 preparation: (i) ebv dna/ebna-1 complexes were specifically enlarged by binding with anti-ebna-1 (ir-3) igg antibody. (ii) dna-binding proteins that did not react with the anti-ebna-1 antibody ... | 1987 | 2821690 |
| punctate outer retinitis in acute epstein-barr virus infection. | | 1987 | 2821813 |
| epstein-barr virus and depression. | | 1987 | 2821836 |
| a model for the development of the tandem repeat units in the ebv ori-p region and a discussion of their possible function. | this paper presents an analysis of the repeat units of the ori-p region of the epstein-barr virus (ebv) genome. these repeat units are well-conserved palindromes. the pattern of these repeats, their lengths, phases, and the distribution of the relatively few substitutions are explained by a scenario that gives a reasonable course for the evolutionary development of the pattern. the scenario suggests a model for the production of an initiating 3/2 palindrome from a moderately lengthy sequence. th ... | 1987 | 2822936 |
| transformation of hairy cell leukemia to ebv genome-containing aggressive b cell lymphoma. | hairy cell leukemia is a preplasmacytic b cell leukemia which is not ebv associated, although elevated titers of epstein-barr virus (ebv) antibodies have been seen in this leukemia and chronic lymphocytic leukemia. hairy cells are not readily susceptible to ebv infection in vitro, even though they are ebv receptor-positive b cells. we have observed a 59-year-old patient who after 9 years of hairy cell leukemia developed a well-differentiated igg-kappa monoclonal b cell lymphoma without further e ... | 1987 | 2823016 |
| virus-induced lymphomas in transplant patients under immunosuppressive therapy. | | 1987 | 2823437 |
| characterization of an epstein-barr virus nuclear antigen 2 variant (ebna 2b) by specific sera. | the jijoye epstein-barr virus (ebv) strain is characterized by a substitution of 1.8 kb in the c-terminal part of ebna 2 gene compared to b95-8 or m-aba virus. protein immunoblot analysis using human sera against ebna 2 indicated that an immunological variant to the ebna 2 of b95-8 (type a) is encoded by the jijoye virus (type b). in order to generate a specific ebna 2b antiserum the naei/nsii dna fragment of the jijoye virus containing 237 bp of the c-terminus from the ebna 2b gene was cloned i ... | 1987 | 2823499 |
| viral antibody titers. comparison in patients with multiple sclerosis and rheumatoid arthritis. | higher titers of antibodies to measles virus envelope antigens, hemolysin and hemagglutinin, epstein-barr virus (ebv) capsid antigen and nuclear antigen, and rubella virus hemagglutinin were demonstrated in serum samples of patients with multiple sclerosis and rheumatoid arthritis than in age- and sex-matched control subjects. a significant correlation was observed between antibodies to measles and rubella viruses both in patients with multiple sclerosis and rheumatoid arthritis, but such a corr ... | 1987 | 2823754 |
| b-cell lymphoma in severe combined immunodeficiency not associated with the epstein-barr virus. | patients with congenital and acquired immunodeficiencies are at increased risk for the development of b-cell lymphoproliferative disorders. when the appropriate tests have been performed, the epstein-barr virus (ebv) nuclear antigen (ebna) or ebv dna has been found in tissues from these tumors. these data have provided support for the idea that these tumors are associated with ebv. in this article we report about a child with severe combined immunodeficiency (scid) who developed a malignant b-ce ... | 1987 | 2824020 |
| differences in b cell growth phenotype reflect novel patterns of epstein-barr virus latent gene expression in burkitt's lymphoma cells. | recently established epstein-barr virus (ebv)-positive burkitt's lymphoma (bl) cell lines, carrying chromosomal translocations indicative of their malignant origin, have been monitored for their degree of in vitro progression towards a more 'lymphoblastoid' cell surface phenotype and growth pattern, and for their expression of three ebv latent gene products which are constitutively present in all virus-transformed normal lymphoblastoid cell lines (lcls). bl cell lines which stably retained the o ... | 1987 | 2824192 |
| non-herpes simplex encephalitis is early exclusion of herpes simplex etiology possible? | since effective antiviral treatment is available for herpes simplex encephalitis (hse), early diagnosis or exclusion of herpes simplex etiology is essential for prognosis. in a retrospective study of 25 cases of acute viral encephalitis not caused by herpes simplex virus (non-hse), we investigated whether hse can be excluded in the early phase before serological evidence is present. using clinical means, history, investigations of csf (protein, cells), eeg, and cct, hse could not be excluded wit ... | 1987 | 2824204 |
| nasopharyngeal carcinoma in israel: epidemiology and epstein-barr virus-related serology. | epidemiological, histological and serological characteristics of nasopharyngeal carcinoma (npc) were investigated. included were 25 patients aged 10-70 with male to female ratio 2:1. among 23 jewish patients, 18 were of asian-african (aa) and five of european (eur) descent; two were arabs (ar). the dominant histological type among aa patients was undifferentiated carcinoma (ucnt) and among eur squamous cell carcinoma (scc). elevated igg and iga antibodies to epstein-barr (ebv) viral capsid, earl ... | 1987 | 2824207 |
| pathways of human b-lymphocyte activation blocked by b-cell specific monoclonal antibodies. | the data presented in this report demonstrate that antibodies directed against b-cell specific surface antigens can block different modes of b-cell activation. the anti-cd 19 antibody hd37 strongly blocks the stimulation of thymidine incorporation induced by anti-mu and mlr supernatant and also partially inhibits the growth of long-term ebv-transformed cell lines. the anti-cd20 antibody b1, on the other hand, has little effect on anti-mu activation but prevents the stimulation of thymidine incor ... | 1987 | 2824347 |
| phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. | natural killer (nk)3 cells are large granular lymphocytes that appear to play a significant role in the host's defense against viral infection. we performed an extensive phenotypic and functional characterization of nk cells on 41 patients with the chronic fatigue syndrome (cfs), or "chronic active epstein-barr virus infection" syndrome, and on 23 age- and sex-matched asymptomatic control subjects in an attempt to further characterize this illness. these studies demonstrated that a majority of p ... | 1987 | 2824604 |
| new type b isolates of epstein-barr virus from burkitt's lymphoma and from normal individuals in endemic areas. | all epstein-barr virus (ebv) isolates can be classified as type a or type b depending upon the identity of their ebv nuclear antigen (ebna) 2 protein. the great majority of isolates examined to date encode an ebna 2a protein like that of the reference type a strain b95-8. type b virus strains, encoding an antigenically distinct ebna 2b protein, have as yet only been rescued from rare burkitt's lymphoma (bl) cell lines of african origin (jijoye, ag876). our recent finding that type b isolates are ... | 1987 | 2824665 |
| detection of antibody to viral proteins following primary infection with herpes simplex virus. | sera from patients with primary genital infection with herpes simplex virus (hsv) were tested by immunoblotting (ib) and radioimmunoprecipitation (rip) analysis to determine the protein targets of antibody elicited by infection. the two tests detected antibody to different antigens: ib primarily detected reactivity with p40, a phosphorylated capsid protein, and rip detected antibody to glycoprotein b, a viral envelope component. furthermore, rip detected antibody at an earlier stage of infection ... | 1987 | 2824685 |
| growth of b95-8 cells and expression of epstein-barr virus lytic phase in serum-free medium. | epstein-barr virus (ebv)-transformed tamarin (saguinus oedipus) cells (b95-8) were selected for growth in medium with reduced serum and then transferred to serum-free medium which consisted of rpmi 1640 supplemented with insulin, transferrin, and selenium. serum-free cells in continuous passage for 1 year had a morphology, growth rate, and culture density which approached those of b95-8 cells grown with serum. the cells expressed virus-induced antigens, including the ebv-associated dna polymeras ... | 1987 | 2824833 |
| in vitro production of human antibodies specifically reactive with human gastric cancer cells of established lines and autologous tissues. | human lymphocytes derived from regional lymph nodes adjacent to the primary gastric cancer were transformed with epstein-barr virus (ebv) to establish lymphoblastoid cell lines secreting human antibodies reactive with cell surface antigens expressed on the gastric cancer cells. the ebv transformation technique was applied to lymph node lymphocytes obtained from 4 gastric cancer patients. as a result of mass screening with the radioactive cell binding assay for the production of anti-gastric canc ... | 1987 | 2824934 |
| viral hepatitis (excluding hepatitis b). | | 1987 | 2825160 |
| epstein-barr virus genomes in lymphoid cells: activation in mitosis and chromosomal location. | biotin-labeled epstein-barr virus (ebv)-specific dna probes have been used to detect viral genomes by in situ hybridization. immunocytochemically amplified signals produced by the hybridized probe allow visualization of viral dna even in cells previously reported to contain only one or two ebv genomes. in ebv producer lymphoid cell lines, such as b95-8, p3hr-1, or daudi, activation of latent ebv dna could be observed in mitotic cells; in non-virus-producing cells of these same lines, ebv was fou ... | 1987 | 2825191 |
| immunologic markers for epstein-barr virus in the control of nasopharyngeal carcinoma and burkitt lymphoma. | immunologic assays have been instrumental in implicating the epstein-barr virus (ebv) as an etiologic factor in nasopharyngeal carcinoma (npc) and burkitt lymphoma (bl). in this report, the importance of a variety of specific assays to detect ebv in tumor biopsies and antibodies to ebv antigens in serum from patients with npc and bl is reviewed. in both npc and bl, the involvement of ebv appears to differ in various geographic locations. therefore, it is necessary to be able to interpret the ava ... | 1987 | 2826001 |
| imbalance of the epstein-barr virus-host relationship in aids-related complex patients. | patients affected by aids related-complex (arc) showed several immunological abnormalities that could lead to a disregulation of immunosurveillance against viral latent infections. we report epstein-barr virus (ebv) reactivation was found in seven of eight arc patients and in two of seven affected by persistent generalized lymphoadenopathy. these patients showed either elevated levels of circulating ebv-positive transformed cells and/or depressed ebv-specific t cell cytotoxicity as assessed by t ... | 1987 | 2826004 |
| epstein-barr virus transformed b cell lines derived from patients with systemic lupus erythematosus produce a nephritic factor of the classical complement pathway. | nephritic factor of the classical complement pathway (c4nef) is an igg antibody which stabilizes the c3 convertase (c4b2a) and has been detected in sera from patients with systemic lupus erythematosus (sle) and acute postinfectious glomerulonephritis. in order to study the production of nephritic factor (nef), mononuclear cells were isolated from the peripheral blood of patients with sle and infected with epstein-barr virus (ebv) to establish active b lymphocyte cell lines. supernatants from 15 ... | 1988 | 2826058 |
| burkitt's lymphoma cell lines reveal different degrees of tumorigenicity in nude mice. | a quantitative in vivo assay for evaluating the tumorigenicity of burkitt's lymphoma (bl) cell lines in nude mice is described. it is based on the dose-response kinetics of bl cell lines in pre-irradiated (480 rad) nude mice following the s.c. injection of 4 different cell doses. this model system was used to estimate the xenografting potential of 26 bl cell lines derived from bl patients of different geographic and ethnic origins, as well as lymphoblastoid cell lines (lcls) established by epste ... | 1988 | 2826348 |
| in vitro generated human monoclonal trinitrophenyl-specific b cell lines. evidence that human and murine anti-trinitrophenyl monoclonal antibodies cross-react with escherichia coli beta-galactosidase. | stable human antigen-specific monoclonal b cell lines were established without prior in vivo immunization. this was accomplished by expanding the anti-trinitrophenyl (tnp) b cells in vitro with the antigen tnp-brucella abortus and then immortalizing them with epstein-barr virus. five anti-tnp clones were selected by sequential limiting dilution. all five anti-tnp clones secreted igm kappa antibodies. when tested against a panel of self and environmental antigens, all five anti-tnp clones exhibit ... | 1987 | 2826579 |
| tumor necrosis factor selectively inhibits activation of human b cells by epstein-barr virus. | we have investigated the effect of recombinant human tumor necrosis factor-alpha (rtnf-alpha) on human b cell activation and differentiation. among several t cell-dependent and independent b cell stimulation systems tested (anti-mu, pokeweed mitogen, epstein-barr virus), only the activation by epstein-barr virus was inhibited by rtnf-alpha. rtnf-alpha inhibited in a dose-dependent manner both the proliferation and differentiation (ig secretion) of epstein-barr virus-stimulated b cells when added ... | 1988 | 2826585 |
| generation of human monoclonal antibodies to cancer-associated antigens using limited numbers of patient lymphocytes. | a limiting dilution method for the efficient transformation by epstein-barr virus (ebv) of human b lymphocytes has been applied to the production of human monoclonal antibodies to ovarian cancer-associated antigens. limited numbers (e.g., 2 x 10(5)) of ebv-infected b lymphocytes from ovarian cancer patient spleen, lymph node, tumor, ascites and blood were successfully transformed using this method. an immunofiltration assay system was employed to identify ebv transformants secreting igm antibody ... | 1987 | 2826600 |
| [a case of epstein-barr virus infection with interstitial pneumonitis]. | | 1987 | 2826863 |
| size heterogeneity of ebv and mitochondrial dnas in burkitt's lymphoma lines. | a simple, reproducible affinity chromatography method has been adapted for separation of high molecular weight supercoiled circular molecules from mammalian cells. electron microscopic analysis of eb viral dna obtained by this method, from the non-producer burkitt's lymphoma line raji, revealed monomer-sized viral molecules only. in contrast, the eb viral episomes from recently established human producer lines bl-8 and ly91 were very heterogeneous in size, some being considerably smaller and oth ... | 1987 | 2827120 |
| potential role of epstein-barr virus in sjögren's syndrome. | sjögren's syndrome is an autoimmune disease characterized by lymphocytic infiltration and destruction of salivary and lacrimal glands. this condition may occur as a primary condition or may be associated with other autoimmune disorders such as rheumatoid arthritis or systemic lupus. because the environmental factors that initiate ss are unknown, we have investigated the potential role of ebv, cmv, and other viruses. we observed that epithelial cells in salivary gland biopsies of patients with ss ... | 1987 | 2827246 |
| herpesviridae in the coronary arteries and aorta of young trauma victims. | the presence of herpes simplex virus (hsv) and cytomegalovirus (cmv) nucleic acid and/or antigen was demonstrated in the coronary arteries and thoracic aortas of young trauma victims by the in situ dna hybridization and abc immunoperoxidase methods, respectively. epstein-barr virus (ebv) nucleic acid and capsid antigen were not detected in the arteries sampled in this study. of 8 subjects in which virus was detected in the coronary arteries, 6 were positive for hsv and 2 for cmv; of 7 cases posi ... | 1988 | 2827495 |
| expression of an early epstein-barr virus antigen (ea-d) in e. coli. brief report. | the 1.34 kb bcii-bgiii-fragment of the bamhi-m region of epstein-barr virus genome, comprising the complete bmrf1 open reading frame, was cloned into the tryptophan regulated e. coli expression vector path1. the resulting fusion protein, having a molecular weight of 80 kd, is recognized not only by anti-early antigen (ea)-positive human sera but also by the monoclonal antibody r3 directed against the diffuse component of ea (ea-d). a possible use for this fusion protein as an indicator protein i ... | 1987 | 2827613 |
| cytochemical, cytogenetic, immunophenotypic and tumorigenic characterization of two hairy cell lines. | two cell lines (eh and hk) were derived from two patients with hairy cell leukemia (hcl). both patients exhibited a clinical picture characteristic of hcl, including splenomegaly, cytopenias, and tartrate-resistant acid phosphatase (trap)-positive "hairy" lymphocytes in blood and marrow. eh and hk were demonstrably of b lineage, as judged by cytochemistry and immunophenotype, including expression of b1, b2, and leu-12 antigens and of monoclonal surface immunoglobulins (slgs). monoclonality was c ... | 1988 | 2827813 |
| in vitro expansion of epstein-barr virus-specific hla-restricted cytotoxic t cells direct from the blood of infectious mononucleosis patients. | the cytotoxic t-cell response induced by primary epstein-barr virus (ebv) infection and detectable in the blood of infectious mononucleosis (im) patients shows several unusual features when tested in in vitro assays. lysis of ebv-transformed target lines occurs with no apparent hla restriction, and the putative ebv specificity of the response has been seriously questioned. in the present work we show that the primary t-cell response in im is polyclonal and indeed does contain a virus-specific hl ... | 1987 | 2828228 |
| interleukin-1-like activity produced by hybrids constructed with epstein-barr-virus-transformed human b lymphocytes and mouse myeloma cells. | peripheral blood b lymphocytes from a donor with positive tuberculin skin test reaction were transformed into lymphoblastoid cell lines by epstein-barr virus and then fused by polyethylene glycol with mouse myeloma cells. human-mouse hybrid cells producing human igm monoclonal antibody to purified protein derivative of tuberculin were isolated, and the concentrated supernatant of one of these cell hybrids was tested for the capacity of interfering with dna synthesis of human and mouse lymphocyte ... | 1988 | 2828240 |
| evaluation of latex-based heterophile antibody assay for diagnosis of acute infectious mononucleosis. | the diagnosis of an acute episode of infectious mononucleosis (im) is made on the basis of clinical symptoms and serological evidence for development of heterophile antibodies. in an effort to obtain a very sensitive and specific assay for diagnosis of im, we have developed meristar:im. the meristar assay uses latex beads coated with purified bovine heterophile antigen. we compared the sensitivity and specificity and performance characteristics of the latex-based test to those of the commerciall ... | 1987 | 2828417 |
| human monoclonal antibody directed against gag gene products of the human immunodeficiency virus. | tonsillar b lymphocytes from an asymptomatic individual infected with hiv were transformed with ebv. a cloned lymphoblastoid cell line was obtained that secreted human mab (igg4 subtype) against the known gag gene products of 55,000 and 25,000 da and a protein of 40,000 da. the latter p40 protein appears to be a hiv gag-related gene product that is also recognized immunologically by individuals infected by hiv. although the mab detects infection of cells by different hiv isolates, it does not ne ... | 1988 | 2828474 |
| viral etiology of testicular tumors. | testicular carcinoma and hodgkin's disease are among the most frequent malignancies afflicting young men in the 15 to 39-year age group. these malignancies share other epidemiological characteristics as well, including multiple histological tumor types, higher rates of occurrence in white, urbanized populations and upper social classes, relative infrequency among black populations, low but definite familial occurrence and an early geographically acquired lifetime risk irrespective of later migra ... | 1988 | 2828695 |
| receptors for insulin and growth hormone on lymphoid cells. | | 1987 | 2828829 |
| establishment and characterization of human leukemia cell lines (j6-1, j6-2 and j6-3). | | 1986 | 2829161 |
| precursor b cells transformed by epstein-barr virus undergo sterile plasma-cell differentiation: j-chain expression without immunoglobulin. | human bone marrow cells were depleted of b lymphocytes to enrich for precursor b cells that could be transformed with epstein-barr virus. transformed immunoglobulin-negative precursors either maintained their immunoglobulin genes in the germ-line configuration or had undergone dj or abortive vdj rearrangements (v, d, and j represent variable, diversity, and joining gene segments). all cell lines and their derivative clones, even those with no detectable immunoglobulin gene rearrangements, genera ... | 1988 | 2829207 |
| increase of protein synthesis by uridine supplement in lectin-stimulated peripheral blood lymphocytes and eb virus-transformed b cell line of hereditary orotic aciduria type i. | a 2 month-old japanese girl with hereditary orotic aciduria type i was treated with oral uridine supplement. the activities of orotate phosphoribosyltransferase (oprt) and orotidine-5'-phosphate decarboxylase (odc) in erythrocytes were 2.7 and 0.4%, respectively, of those in the controls. megaloblastic anemia, excessive urinary excretion of orotic acid, lymphopenia and decreased number of okt3 positive lymphocytes on admission were corrected after the uridine supplement. peripheral blood lymphoc ... | 1987 | 2829387 |
| tpa-inducible epstein-barr virus genes in raji cells and their regulation. | a number of agents including the tumor promoter 12-o-tetradecanoylphorbol-13-acetate (tpa) can induce an abortive virus cycle in the ebv nonproducer burkitt's lymphoma line raji. we describe the pattern of viral rnas transcribed in uninduced cells and in cells treated with tpa for 8 hr, as analyzed by northern blotting. by comparing the patterns of rnas observed in cells treated with tpa, tpa plus cycloheximide, or cycloheximide alone, we have tested whether any ebv gene in tpa-treated raji cell ... | 1988 | 2829433 |
| neutrophil lactoferrin content in viral infections. | in an attempt to elucidate the previously observed decrease in plasma lactoferrin-neutrophil ratio in subjects with acute viral infections, a study of the neutrophil lactoferrin content in such infections was undertaken. with the use of an immunoperoxidase stain for lactoferrin, neutrophils in viral illness were shown to have reduced lactoferrin content (mean score 97.9 +/- sd [38.0] per 100 neutrophils) as compared with normal subjects (mean score 196.4 +/- sd [3.6] per 100 neutrophils) (t = 7. ... | 1988 | 2829617 |
| chronic fatigue syndrome: a working case definition. | the chronic epstein-barr virus syndrome is a poorly defined symptom complex characterized primarily by chronic or recurrent debilitating fatigue and various combinations of other symptoms, including sore throat, lymph node pain and tenderness, headache, myalgia, and arthralgias. although the syndrome has received recent attention, and has been diagnosed in many patients, the chronic epstein-barr virus syndrome has not been defined consistently. despite the name of the syndrome, both the diagnost ... | 1988 | 2829679 |
| granuloma annulare-like eruption due to chronic epstein-barr virus infection. | a 32-year-old woman afflicted with a severe, chronic epstein-barr virus (cebv) infection of 12 months' duration developed an unusual, granuloma annulare-like eruption. the cutaneous disorder, which paralleled the clinical course of her disease, was characterized by slightly raised, erythematous, annular lesions predominantly involving the face and arms, with sporadic involvement elsewhere. histopathologic examination of biopsy material obtained from affected skin disclosed a granulomatous dermat ... | 1988 | 2829748 |
| [detection of epstein-barr virus nucleic acid sequences in non-hodgkin's lymphoma]. | tumor tissue specimens of 25 patients with various entities of non-hodgkin's lymphomas were examined for the presence of epstein-barr virus (ebv) nucleic acids and ebv nuclear antigens (ebna) by in situ hybridization and anticomplement immunofluorescence. in tumor cells of five patients ebv nucleic acid and ebna were demonstrated. the histopathologic examinations revealed in these cases one burkitt's lymphoma, two centroblastic lymphomas and each one lymphoplasmocytic and lymphocytic lymphoma. | 1987 | 2829788 |
| effects of human immunodeficiency virus (hiv) on the cytotoxic response to epstein barr virus (ebv) transformed b lymphocytes. | the human immunodeficiency virus (hiv) may interact with the epstein barr virus (ebv) indirectly by effects on the t4 lymphocyte or directly by effects on ebv transformed b lymphocytes. we have confirmed the susceptibility of ebv transformed b lymphocytes to productive hiv infection, and have evaluated the cytotoxic activity of hiv seronegative and seropositive donors after sensitization by their autologous ebv infected (monoinfected) or ebv and hiv infected (coinfected) transformed cell lines i ... | 1987 | 2829951 |
| an ebv-based mammalian cell expression vector for efficient expression of cloned coding sequences. | the construction of a mammalian cell expression vector using human cytomegalovirus immediate early gene enhancer to initiate transcription of inserted coding sequences is described. the vector also carries epstein-barr virus ebna-1 nuclear antigen gene, ori-p sequences and hygromycin b resistance gene hph from e. coli. the expression capacity of this construct was tested by inserting the chloramphenicol acetyltransferase (cat) gene into the vector. the ebv-cat construct was transfected into vari ... | 1988 | 2829966 |
| b lymphoblastoid cell lines with rearranged t cell receptor beta-chain genes retain conventional b cell surface antigen and ig expression. | transformation of peripheral blood lymphocytes by co-incubation with ebv produces b lymphoblastoid cell lines, but rearrangement of tcr beta-chain genes was observed in three different cell lines derived from two individuals. because rearrangement of tcr genes in b lymphocytes is considered a rare event, these b lymphoblastoid cell lines with rearranged tcr beta-genes were examined in detail to determine whether there were any additional characteristics to distinguish them from b lymphoblastoid ... | 1988 | 2830338 |
| acute cerebellar syndrome complicating infectious mononucleosis. | | 1987 | 2830369 |
| epstein-barr virus and epithelial cells: implications for the otolaryngologist. | epstein-barr virus (ebv) is an oncogenic herpesvirus associated with two human malignancies. initially linked in the etiology of burkitt's lymphoma, a b cell lymphoma, it has subsequently been implicated in the causation of nasopharyngeal carcinoma, an epithelial tumor of considerable world health importance. more recently a number of head and neck tumors have been shown to harbor the virus, suggesting a possible role for ebv in their development. | 1988 | 2830415 |
| cytotoxic t-cell clones discriminate between a- and b-type epstein-barr virus transformants. | epstein-barr virus (ebv) is the aetiological agent of infectious mononucleosis and is associated with burkitt's lymphoma and nasopharyngeal carcinoma. the virus is harboured for life in all previously infected individuals and is apparently controlled by a population of ebv-specific memory t lymphocytes, specifically activated to recognize the functionally defined lymphocyte-detected membrane antigen. two types (a and b) of ebv have been identified that show dna sequence divergence within the bam ... | 1988 | 2830541 |
| reactivation of epstein-barr virus in sjögren's syndrome. | sjögren's syndrome (ss) is an autoimmune disease characterized by lymphocytic infiltration and destruction of salivary and lacrimal glands. this condition may be limited to glandular tissues or may be associated with other autoimmune disorders such as rheumatoid arthritis or systemic lupus erythematosus. since the environmental factors that initiate ss are unknown, we have investigated the potential role of epstein-barr virus (ebv), cytomegalovirus (cmv) and other viruses. we observed that epith ... | 1987 | 2830658 |
| a gene for dihydrofolate reductase in a herpesvirus. | the enzyme dihydrofolate reductase (dhfr) is found ubiquitously in both prokaryotes and eukaryotes. it is essential for de novo synthesis of purines and of deoxythymidine monophosphate for dna synthesis. among viruses, however, only the t-even and t5 bacteriophage have been found to encode their own dhfr. in this study a gene for dhfr was found in a specific subgroup of the gamma or lymphotropic class of herpesviruses. dna sequences for dhfr were found in herpesvirus saimiri and herpesvirus atel ... | 1988 | 2830673 |
| mechanism of activation and biological role of the c-myc oncogene in b-cell lymphomagenesis. | | 1987 | 2830825 |
| studies on cell-mediated immune defects to epstein-barr virus and cytomegalovirus in hiv-related disorders. | | 1987 | 2830827 |
| serological follow-up study on the antibody levels to epstein-barr virus-determined nuclear antigen (ebna) patients with nasopharyngeal carcinoma (npc) after radiation therapy. | serological follow-up studies for up to 4 years on the levels of igg antibody to ebv-determined nuclear antigen (ebna) were carried out on 36 patients with nasopharyngeal carcinoma (npc). the serum levels of iga antibody specific to ebv capsid antigen (vca) were also measured in some of the patients. the titers of ebna antibody were measured by enzyme-linked immunosorbent assay (elisa) and those of iga antibody to vca were measured by the indirect immunofluorescence method. the ebna antibody tit ... | 1987 | 2830879 |
| mycobacterium leprae-reactive t-cell clones isolated from polar lepromatous and tuberculoid leprosy patients. | t-cell clones capable of mounting a proliferative response to mycobacterium leprae were obtained in three leprosy patients (two polar lepromatous and one polar tuberculoid) either from m. leprae-activated or from protein-purified derivative-activated polyclonal t lymphoblasts. all these clones expressed the cd4 surface marker. some of them proliferated to the antigen only in the presence of interleukin-2. a majority expressed cross-reactive responses to other mycobacteria. clones obtained from t ... | 1987 | 2830893 |
| polyspecific natural antibodies and autoantibodies secreted by human lymphocytes immortalized with epstein-barr virus. | recent studies have shown that autoreactive b cells and autoantibodies are present in pathological as well as in normal situations. in the present study, we immortalized human b cell lines from normal individuals and from patients with malignant or benign dysglobulinemia with epstein-barr virus and examined, after cloning, the autoantibody reactivities of the immunoglobulins secreted by these cells. forty-two supernatants were analyzed by enzyme-immunoassay on a panel of 13 self and non-self ant ... | 1988 | 2830925 |
| subclass of individual iga-secreting human lymphocytes. investigation of in vivo pneumococcal polysaccharide-induced and in vitro mitogen-induced blood b cells by monolayer plaque-forming cell assays. | the subclass of individual human iga b cells was investigated by means of monolayer plaque-forming cell assays permitting analysis of all iga-secreting cells as well as of cells secreting iga anti-pneumococcal polysaccharide antibody. center cells were examined by indirect immunofluorescence staining with mouse mab against either of the two iga subclasses as primary antibodies and fitc-conjugated rabbit anti-mouse ig as the second antibody. blood lymphocytes spontaneously secreting iga (mean 399 ... | 1988 | 2831268 |
| [transfection of human nasopharyngeal epithelial cells with epstein-barr virus dna derived from nasopharyngeal carcinoma]. | | 1987 | 2831323 |
| a fifth epstein-barr virus nuclear protein (ebna3c) is expressed in latently infected growth-transformed lymphocytes. | three distantly homologous neighboring long open reading frames in the epstein-barr virus (ebv) genome are preceded by short open reading frames. the leftmost short and long open reading frames encode ebna3, a nuclear protein which is slightly smaller (145 kilodaltons [kda]) than two other nuclear proteins (150 to 155 kda) detected in western blots (immunoblots) of latently infected cell protein (k. hennessy, f. wang, e. woodland-bushman, and e. kieff, proc. natl. acad. sci. usa 83:5693-5697, 19 ... | 1988 | 2831394 |
| intertypic cross-neutralization of polioviruses by human monoclonal antibodies. | human b cell lines producing monoclonal antibodies (mabs) reactive with poliovirus type 1 were generated by transformation with epstein-barr virus (ebv) b 95-8 of tonsillar lymphocytes from several immune donors. ebv-transformed cells were cloned in semisolid agarose. some neutralizing (nt) human mabs recognized and neutralized only poliovirus type 1, whereas other nt mabs neutralized either poliovirus type 1 and 2 or all three serotypes. mabs reactive with poliovirus type 1 and 3 but not with t ... | 1988 | 2831657 |
| [the relation between age and cytomegalovirus antibodies and eb virus-associated antibodies]. | | 1987 | 2832639 |
| color graphics representations of large sequences in the gem environment. | the analytical and descriptive color graphics capabilities (the gem environment) of the cage/gem(a) software system are described using bacteriophage lambda (48,502 base pairs) and epstein barr virus (172,282 base pairs) as examples. genetics and features, as graphic drawings, and the results of sequence analysis as pseudo-colored representations, are simultaneously displayed at all zooming levels. the present upper limit on sequence size is 5 x 10(5) bases. the overlay editor utility which prov ... | 1988 | 2832825 |
| development of an enzyme-linked immunosorbent assay (elisa) for detecting iga antibodies to the epstein-barr virus. | a 3-step enzyme-linked immunosorbent assay (elisa) was developed for detecting iga antibodies to purified epstein-barr virus (ebv) polypeptides. the 3-step procedure included the use of a mouse anti-human iga monoclonal antibody (mab) to amplify the iga reaction. the 2 major ebv proteins used in this assay were the 125-kda component (gp125) associated with the viral capsid antigen (vca) complex and a major glycoprotein (gp250/200) associated with the membrane antigen (ma) complex. eighty-two ser ... | 1988 | 2833449 |
| [recover of transforming epstein-barr virus (ebv) from raji cells superinfected with npc-kt ebv]. | | 1987 | 2833593 |
| adenovirus type-2 in a patient with lethal hemorrhagic colonic ulcers and chronic active epstein-barr virus infection. | an adenovirus type-2 was isolated from peripheral blood lymphocytes and throat washings from a patient with severe chronic active epstein-barr virus infection. despite the epstein-barr virus reactivation, attempts to establish spontaneous lymphoblastoid cell lines from peripheral blood lymphocytes and to immortalize cord lymphocytes with throat washings were unsuccessful due to a marked cytopathic effect. the supernatants from the cultures induced cytopathic effect in cultured cord lymphocytes, ... | 1988 | 2833864 |
| screening of edible plants against possible anti-tumor promoting activity. | one hundred twenty-one species of edible plants (133 test-parts) were screened against possible anti-tumor promoting activity by an in vitro short-term assay system of inhibition of epstein-barr virus (ebv) activation induced by a phorbol-ester promoter, 12-o-hexadecanoylphorbol-13-acetate (hpa). the methanol-extracts (me) of 14 species of the edible plants strongly inhibited the activation, 7 moderately and 12 weakly inhibited it. on partition of the randomly selected inactive me (26 species) w ... | 1988 | 2834042 |