| biodiversity and γ-aminobutyric acid production by lactic acid bacteria isolated from traditional alpine raw cow's milk cheeses. | "nostrano-cheeses" are traditional alpine cheeses made from raw cow's milk in trentino-alto adige, italy. this study identified lactic acid bacteria (lab) developing during maturation of "nostrano-cheeses" and evaluated their potential to produce γ-aminobutyric acid (gaba), an immunologically active compound and neurotransmitter. cheese samples were collected on six cheese-making days, in three dairy factories located in different areas of trentino and at different stages of cheese ripening (24 ... | 2015 | 25802859 |
| evaluation of apically extruded bacteria associated with different nickel-titanium systems. | the aim of this in vitro study was to evaluate the amount of apically extruded bacteria associated with several root canal preparation systems. | 2015 | 25799537 |
| enterococcus species diversity and molecular characterization of biomarker genes in enterococcus faecalis in port blair bay, andaman and nicobar islands, india. | this study was performed to evaluate the abundance and diversity of enterococcus sp. and the distribution of biomarker genes in enterococcus faecalis in port blair bay, andaman and nicobar islands. the enterococcus sp. densities at the seven sampling stations were highly influenced by tidal fluctuations and season. the distributions and diversities of species varied in the inner and outer regions of port blair bay. among the 1816 total isolates, the occurrence of fecal enterococcus was high (1.7 ... | 2015 | 25798888 |
| antibacterial and antioxidant xanthones and benzophenone from garcinia smeathmannii. | a new prenylated xanthone, 1,3,5,8-tetrahydroxy-2-(3-methybut-2-enyl)-4-(3,7-dimethylocta-2,6-dienyl) xanthone (1), and a new benzophenone (2), together with four known xanthone derivatives, cheffouxanthone (3), smeathxanthone a (4), smeathxanthone b (5), ananixanthone (6), and two pentacyclic triterpenes, epi-friedelinol (7) and friedelin (8), were isolated from the stem bark of garcinia smeathmannii. the structures of the compounds were elucidated on the basis of 1d and 2d nmr experiments, and ... | 2015 | 25798641 |
| detection of vancomycin-resistant enterococcus faecalis st6-vanb2 and e. faecium st915-vana in faecal samples of wild rattus rattus in spain. | the detection of vancomycin-resistant-enterococci (vre) among wild animals represents a worrisome public health concern. the objectives of the study were to determine the possible presence of vre in faecal samples of wild small mammals in spain, to characterize the vancomycin resistance mechanisms and genetic lineages of recovered isolates and to know the diversity of enterococcal species in these animals. a total of 155 faecal samples from small mammals were inoculated in slanetz-bartley agar s ... | 2015 | 25795517 |
| antimicrobial activity of seven essential oils from iranian aromatic plants against common causes of oral infections. | over the past two decades, there has been a growing trend in using oral hygienic products originating from natural resources such as essential oils (eos) and plant extracts. seven aromatic plants used in this study are among popular traditional iranian medicinal plants with potential application in modern medicine as anti-oral infectious diseases. | 2015 | 25793100 |
| biological evaluation and molecular modelling study of thiosemicarbazide derivatives as bacterial type iia topoisomerases inhibitors. | in the present article, we describe the inhibitory potency of nine thiosemicarbazide derivatives against bacterial type iia topoisomerases, their antibacterial profile and molecular modelling evaluation. we found that one of the tested compounds, compound 7, significantly inhibits activity of staphylococcus aureus dna gyrase with an ic(50) below 15 μm. besides, this compound displays antibacterial activity on reference staphylococuss spp. and enterococcus faecalis strains as well as clinical s. ... | 2016 | 25792505 |
| cytotoxic and antibacterial angucycline- and prodigiosin-analogues from the deep-sea derived streptomyces sp. scsio 11594. | two new c-glycoside angucyclines, marangucycline a (1) and marangucycline b (2), along with three known compounds, dehydroxyaquayamycin (3), undecylprodigiosin (4) and metacycloprodigiosin (5), have been identified as products of the deep-sea sediment strain streptomyces sp. scsio 11594. new structures were elucidated on the basis of hresims, 1d and 2d nmr analyses and comparisons to previously reported datasets. compounds 2 and 4 displayed in vitro cytotoxicity against four cancer cell lines a5 ... | 2015 | 25786061 |
| antimicrobial activity of plectasin nz2114 in combination with cell wall targeting antibiotics against vana-type enterococcus faecalis. | antimicrobial peptide plectasin targeting bacterial cell wall precursor lipid ii has been reported to be active against benzylpenicillin-resistant streptococcus pneumoniae but less potent against vancomycin-resistant enterococci than their susceptible counterparts. the aim of this work was to test plectasin nz2114 in combination with cell wall targeting antibiotics on vancomycin-resistant enterococcus faecalis. the activity of antibiotic combinations was evaluated against vana-type vancomycin-re ... | 2015 | 25785733 |
| hydrogel-based microfluidic incubator for microorganism cultivation and analyses. | this work presents an array of microfluidic chambers for on-chip culturing of microorganisms in static and continuous shear-free operation modes. the unique design comprises an in-situ polymerized hydrogel that forms gas and reagent permeable culture wells in a glass chip. utilizing a hydrophilic substrate increases usability by autonomous capillary priming. the thin gel barrier enables efficient oxygen supply and facilitates on-chip analysis by chemical access through the gel without introducin ... | 2015 | 25784966 |
| increased membrane surface positive charge and altered membrane fluidity leads to cationic antimicrobial peptide resistance in enterococcus faecalis. | to understand the role of cell membrane phospholipids during resistance development to cationic antimicrobial peptides (camps) in enterococcus faecalis, gradual dose-dependent single exposure pediocin-resistant (pedr) mutants of e. faecalis (efv2.1, efv3.1, efv3.2, efv4.1, efv4.2, efv5.1, efv5.2 and efv5.3), conferring simultaneous resistance to other camps, selected in previous study were characterized for cell membrane phospholipid head-groups and fatty acid composition. the involvement of pho ... | 2015 | 25782727 |
| unraveling antimicrobial resistance genes and phenotype patterns among enterococcus faecalis isolated from retail chicken products in japan. | multidrug-resistant enterococci are considered crucial drivers for the dissemination of antimicrobial resistance determinants within and beyond a genus. these organisms may pass numerous resistance determinants to other harmful pathogens, whose multiple resistances would cause adverse consequences. therefore, an understanding of the coexistence epidemiology of resistance genes is critical, but such information remains limited. in this study, our first objective was to determine the prevalence of ... | 2015 | 25781022 |
| a genomic virulence reference map of enterococcus faecalis reveals an important contribution of phage03-like elements in nosocomial genetic lineages to pathogenicity in a caenorhabditis elegans infection model. | in the present study, the commensal and pathogenic host-microbe interaction of enterococcus faecalis was explored using a caenorhabditis elegans model system. the virulence of 28 e. faecalis isolates representing 24 multilocus sequence types (mlsts), including human commensal and clinical isolates as well as isolates from animals and of insect origin, was investigated using c. elegans strain glp-4 (bn2ts); sek-1 (km4). this revealed that 6 e. faecalis isolates behaved in a commensal manner with ... | 2015 | 25776747 |
| efficacy of low concentrations of sodium hypochlorite and low-powered er,cr:ysgg laser activated irrigation against an enterococcus faecalis biofilm. | to establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without er,cr:ysgg laser activation on enterococcus faecalis biofilms in extracted teeth. | 2016 | 25772335 |
| rapid fluorescence-based measurement of toxicity in anaerobic digestion. | a rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. by initially using a pure facultative anaerobic strain, enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (pcp). the technique revealed significant metabolic changes in enterococcus faecalis with a pcp spike ranging from 0.05 to 100 mg/l, and ... | 2015 | 25768985 |
| could β-hemolytic, group b enterococcus faecalis be mistaken for streptococcus agalactiae? | a β-hemolytic enterococcus faecalis strain agglutinating lancefield group a, b, c, d, f, and g antisera was observed from a rectovaginal swab, in the context of antenatal screening for streptococcus agalactiae (group b streptococcus [gbs]). this is the first multi-lancefield antisera-agglutinating isolate of this species, and it raised particular concern, as it may mimic gbs, leading to false reporting and useless receipt of intrapartum antibiotics. | 2015 | 25766004 |
| re: enterococcus faecalis subverts and invades the host urothelium in patients with chronic urinary tract infection. | | 2015 | 25765392 |
| bacteriological findings and antimicrobial susceptibility pattern of isolated pathogens from visual threatening ocular infections. | to study the profiles and antimicrobial susceptibility pattern of isolated bacteria from visual threatening ocular infection. | 2015 | 25764616 |
| fluoroquinolone resistance in bacteremic and low risk febrile neutropenic patients with cancer. | the low risk febrile neutropenic patients with multinational association for supportive care in cancer (mascc) score of more than 20 are recommended to be treated with fluoroquinolone-based oral treatment by the national comprehensive cancer network (nccn) guideline. this recommendation relies, at least partially, on the high sensitivity of the blood culture isolates to fluoroquinolone in clinical trials conducted in western countries. whether this also applies in middle or low income countries ... | 2015 | 25763661 |
| antimicrobial action of oleanolic acid on listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. | this study investigated the antimicrobial action of oleanolic acid against listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. to determine the cytotoxicity of oleanolic acid, hep-2 cells were incubated with oleanolic acid at 37°c. mics (minimal inhibition concentrations) for l. monocytogenes, e. faecium, and e. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid a ... | 2015 | 25756202 |
| β-lactams enhance daptomycin activity against vancomycin-resistant enterococcus faecalis and enterococcus faecium in in vitro pharmacokinetic/pharmacodynamic models. | enterococcus faecalis and enterococcus faecium are frequently resistant to vancomycin and β-lactams. in enterococcal infections with reduced glycopeptide susceptibility, combination therapy is often administered. our objective was to conduct pharmacokinetic/pharmacodynamic (pk/pd) models to evaluate β-lactam synergy with daptomycin (dap) against resistant enterococci. one e. faecalis strain (r6981) and two e. faecium strains (r6370 and 8019) were evaluated. dap mics were obtained. all strains we ... | 2015 | 25753639 |
| fluid dynamics and biofilm removal generated by syringe-delivered and 2 ultrasonic-assisted irrigation methods: a novel experimental approach. | thorough understanding of fluid dynamics in root canal irrigation and corresponding antibiofilm capacity will support improved disinfection strategies. this study aimed to develop a standardized, simulated root canal model that allows real-time analysis of fluid/irrigation dynamics and its correlation with biofilm elimination. | 2015 | 25749254 |
| ag-loaded mesoporous bioactive glasses against enterococcus faecalis biofilm in root canal of human teeth. | ag-loaded mesoporous bioactive glass (ag-mbg) powders were synthesized and characterized. the ions release of ag-mbgs in tris-hcl and the ph stability of simulated body fluids after immersing ag-mbgs were tested. root canals were inoculated with enterococcus faecalis for 4 weeks, and the antibacterial activity of mbgs, ag-mbgs and calcium hydroxide against e. faecalis biofilm were evaluated. results showed that ag-mbgs possessed highly ordered mesoporous structure with silver nanoparticles depos ... | 2015 | 25748459 |
| [analysis of pathogenic bacteria and drug resistance in neonatal purulent meningitis]. | to study the clinical characteristics, pathogenic bacteria, and antibiotics resistance of neonatal purulent meningitis in order to provide the guide for early diagnosis and appropriate treatment. | 2015 | 25748405 |
| molecular mechanisms of resistance to human pathogenic bacteria in caenorhabditis elegans by mev-1 mediated oxidative stress. | both mutation and knockdown in mev-1 gene render caenorhabditis elegans susceptibility to enterococcus faecalis infection. however, the mechanisms by which of mev-1 defects pathogen resistance remain unclear. here we show that mev-1rnai causes a dramatic decrease in oxidative stress and antioxidant enzyme expressions, thereby leading to increased e. faecalis accumulation in nematode intestine. mitochondrial superoxide change after infection induced these oxidative responses through daf-16 activi ... | 2015 | 25747713 |
| new antimicrobial contact catalyst killing antibiotic resistant clinical and waterborne pathogens. | microbial growth on medical and technical devices is a big health issue, particularly when microorganisms aggregate to form biofilms. moreover, the occurrence of antibiotic-resistant bacteria in the clinical environment is dramatically growing, making treatment of bacterial infections very challenging. in search of an alternative, we studied a novel antimicrobial surface coating based on micro galvanic elements formed by silver and ruthenium with surface catalytic properties. the antimicrobial c ... | 2015 | 25746238 |
| the effect of indomethacin and betamethasone on the cytokine response of human neonatal mononuclear cells to gram-positive bacteria. | intrauterine infections with gram-positive bacteria pose a serious threat to neonates since they can result in neonatal sepsis, induce a fetal inflammatory response and also cause preterm birth. despite intensive care, prematurity remains a leading cause of neonatal death, and is often accompanied by a number of morbidities. in order to prevent premature birth, tocolytic agents like indomethacin are administered. betamethasone is used to promote lung maturation and prevent respiratory distress s ... | 2015 | 25743243 |
| whole-genome mapping of 5' rna ends in bacteria by tagged sequencing: a comprehensive view in enterococcus faecalis. | enterococcus faecalis is the third cause of nosocomial infections. to obtain the first snapshot of transcriptional organizations in this bacterium, we used a modified rna-seq approach enabling to discriminate primary from processed 5' rna ends. we also validated our approach by confirming known features in escherichia coli. we mapped 559 transcription start sites (tsss) and 352 processing sites (psss) in e. faecalis. a blind motif search retrieved canonical features of siga- and sign-dependent p ... | 2015 | 25737579 |
| the fsr quorum-sensing system and cognate gelatinase orchestrate the expression and processing of proprotein ef_1097 into the mature antimicrobial peptide enterocin o16. | a novel antimicrobial peptide designated enterocin o16 was purified from enterococcus faecalis. mass spectrometry showed a monoisotopic mass of 7,231 da, and n-terminal edman degradation identified a 29-amino-acid sequence corresponding to residues 90 to 119 of the ef_1097 protein. bioinformatic analysis showed that enterocin o16 is composed of the 68 most c-terminal residues of the ef_1097 protein. introduction of an in-frame isogenic deletion in the ef1097 gene abolished the production of ente ... | 2015 | 25733609 |
| ec300: a phage-based, bacteriolysin-like protein with enhanced antibacterial activity against enterococcus faecalis. | bacteriophage lytic enzymes, either endolysins or virion-associated lysins, have been receiving considerable attention as potential antibacterial agents, particularly for the combat of antibiotic-resistant gram-positive pathogens. a conclusion that easily emerges from the careful analysis of a great number of reports on the field is that the activity of phage lytic enzymes is rarely studied in conditions that support robust growth of the target bacteria. here, we report the construction and stud ... | 2015 | 25728445 |
| the sfani restriction-modification system from enterococcus faecalis neb215 is located on a putative mobile genetic element. | a type iis restriction-modification (r-m) system sfani from enterococcus faecalis neb215 has been characterized. the sfanim gene was cloned by the methylase selection method. methyltransferase sfani, a protein of 695 amino acids, consists of two domains responsible for different dna-strand recognition and modification, and a putative dna-binding hth domain located in the n-terminal part of the protein. the sfanir gene, located adjacent to the gene of the cognate modification methyltransferases, ... | 2015 | 25724535 |
| validation of a novel rinse and filtration method for efficient processing of fresh produce samples for microbiological indicator enumeration. | several methods have been described to prepare fresh produce samples for microbiological analysis, each with its own advantages and disadvantages. the aim of this study was to compare the performance of a novel combined rinse and membrane filtration method to two alternative sample preparation methods for the quantification of indicator microorganisms from fresh produce. decontaminated cantaloupe melons and jalapeño peppers were surface inoculated with a cocktail containing 10(6) cfu/ml escheric ... | 2015 | 25719876 |
| antibacterial and smear layer removal capability of oregano extract solution. | the aim of this in vitro study was to evaluate the antimicrobial effect of oregano extract solution (oes) against enterococcus faecalis within root canals and dentin tubules, and its effect on smear layer. | 2017 | 25713479 |
| in vitro activity of gentamicin, vancomycin or amikacin combined with edta or l-arginine as lock therapy against a wide spectrum of biofilm-forming clinical strains isolated from catheter-related infections. | treatment of catheter-related bloodstream infections (crbsi) is hampered by the characteristic tolerance of bacterial biofilms towards antibiotics. our objective was to study the effect of the combination of antibiotics and the alkaline amino acid l-arginine or the cation chelator edta on the bacterial killing of in vitro biofilms formed by an array of clinical strains responsible for crbsi and representative of epidemiologically relevant bacterial species. | 2015 | 25712314 |
| spread of an enterococcus faecalis sequence type 6 (cc2) clone in patients undergoing selective decontamination of the digestive tract. | enterococcus faecalis (e. faecalis) is a common cause of nosocomial infection in immunocompromised patients. the presence and dissemination of high-risk clonal complexes, such as cc2, is an ongoing problem in hospitals. the aim of this work was to characterize 24 e. faecalis isolates from icu patients undergoing selective decontamination of the digestive tract (sdd) by phenotypical (antimicrobial susceptibility) and genotypical (presence of virulence genes, rapd-pcr and mlst) methods. our result ... | 2015 | 25712203 |
| antimicrobial activity of root canal irrigants associated with cetrimide against biofilm and planktonic enterococcus faecalis. | to evaluate the antibacterial activity of sodium hypochlorite (naocl) and chlorhexidine (chx) alone or associated with cetrimide (ctr), and qmix against biofilm and planktonic enterococcus faecalis (e. faecalis) [american type culture collection (atcc) 29212]. | 2014 | 25707833 |
| phage-mediated dispersal of biofilm and distribution of bacterial virulence genes is induced by quorum sensing. | the microbiome and the phage meta-genome within the human gut are influenced by antibiotic treatments. identifying a novel mechanism, here we demonstrate that bacteria use the universal communication molecule ai-2 to induce virulence genes and transfer them via phage release. high concentrations (i.e. 100 μm) of ai-2 promote dispersal of bacteria from already established biofilms, and is associated with release of phages capable of infecting other bacteria. enterococcus faecalis v583δabc harbour ... | 2015 | 25706310 |
| is it safe to reuse dental implant healing abutments sterilized and serviced by dealers of dental implant manufacturers? an in vitro sterility analysis. | this study was undertaken to investigate the sterility of used healing abutments sterilized and serviced by dealers of dental implant manufacturers. | 2015 | 25706262 |
| vancomycin-resistant enteroccus faecium and enterococcus faecalis isolated from education hospital of iran. | enterococci are opportunistic pathogens which represent one of the leading agents of nosocomial infections, especially urinary tract infections (uti) in hospitalized patients. the aim of the present study was to determine the resistance pattern and the type of resistance genes in vancomycin-resistant enterococcus isolated from an educational hospital in iran. | 2014 | 25705299 |
| novel coating of surgical suture confers antimicrobial activity against porphyromonas gingivalis and enterococcus faecalis. | the oral cavity is colonized by >10(9) bacteria, many of which can increase heart disease risk when seeded into the bloodstream. most dentoalveolar surgeries require the use of surgical sutures. suture placement and removal can increase the risk of postoperative infection and bacteremia. the aim of this study is to evaluate the antimicrobial activity of a novel quaternary ammonium compound, k21, when coated on different suture materials. | 2015 | 25703732 |
| ultrasonic activation and chemical modification of photosensitizers enhances the effects of photodynamic therapy against enterococcus faecalis root-canal isolates. | the aim of this study was to evaluate the effect of photodynamic therapy (pdt) on enterococcus faecalis biofilms in artificially infected root canals using modified photosensitizers and passive ultrasonic activation. | 2015 | 25703104 |
| biofilms of enterococcus faecalis and enterococcus faecium isolated from the processing of ricotta and the control of these pathogens through cleaning and sanitization procedures. | the biofilm formation of enterococcus faecalis and enterococcus faecium isolated from the processing of ricotta on stainless steel coupons was evaluated, and the effect of cleaning and sanitization procedures in the control of these biofilms was determined. the formation of biofilms was observed while varying the incubation temperature (7, 25 and 39°c) and time (0, 1, 2, 4, 6 and 8 days). at 7°c, the counts of e. faecalis and e. faecium were below 2 log10 cfu/cm(2). for the temperatures of 25 an ... | 2015 | 25702883 |
| effects of ciprofloxacin-containing scaffolds on enterococcus faecalis biofilms. | antibiotic-containing polymer-based nanofibers (hereafter referred to as scaffolds) have demonstrated great potential for their use in regenerative endodontics from both an antimicrobial and cytocompatibility perspective. this study sought to evaluate in vitro the effects of ciprofloxacin (cip)-containing polymer scaffolds against enterococcus faecalis biofilms. | 2015 | 25698261 |
| [inferior vena cava thrombosis and endocarditis in a premature patient: case report]. | thrombosis of the inferior vena cava represents a significant percentage of all venous thrombosis that take place during the neonatal period, generally associated with risk factors such as the use of central venous catheter. the incidence of bacterial endocarditis in preterm infants is low. | 2014 | 25697617 |
| nonselective mevalonate kinase inhibitor as a novel class of antibacterial agents. | introduction. there are a few evidences about targeting isoprenoids biosynthesis pathway in bacteria for finding new antibiotics. this study was conducted to assess antibacterial effects of vanadyl sulfate (vs), one of the mevalonate kinase inhibitors to find a new target for killing bacteria. materials and methods. antibacterial effect of vs alone and in combination with glycine or edta was assessed on escherichia coli and pseudomonas aeruginosa as gram-negative and staphylococcus aureus and en ... | 2015 | 25692035 |
| the effect of diluted triple and double antibiotic pastes on dental pulp stem cells and established enterococcus faecalis biofilm. | to investigate the effect of various dilutions of antibiotic medicaments used in endodontic regeneration on the survival of human dental pulp stem cells (dpscs) and to determine their antibacterial effect against established enterococcus faecalis biofilm. | 2015 | 25689981 |
| orosomucoid 1 drives opportunistic infections through the polarization of monocytes to the m2b phenotype. | orosomucoid (orm, composed of two isoforms, orm1 and orm2) has been described as an inducer of m2 macrophages, which are cells that decrease host antibacterial innate immunities. however, it is unknown which phenotypes of m2 macrophages are induced by orm. in this study, healthy donor monocytes stimulated with orm (orm-monocytes) were characterized phenotypically and biologically. ccl1 (a biomarker of m2b macrophages) and il-10 were detected in monocyte cultures supplemented with orm1; however, ... | 2015 | 25689617 |
| growth-dependent photoinactivation kinetics of enterococcus faecalis. | to investigate how the growth stage of enterococcus faecalis affects its photoinactivation in clear water. | 2015 | 25688992 |
| [synergistic effects of lysozyme with edta-2na on antibacterial activity]. | to evaluate the synergistic antibacterial effects of lysozyme with ethylenediaminetetraacetic acid disodium salt (edta-2na) on enterococcus faecalis (e. faecalis) and porphyromonas endodontalis (p. endodontalis). | 2015 | 25686329 |
| antimicrobial peptides expressed in medicinal maggots of the blow fly lucilia sericata show combinatorial activity against bacteria. | the larvae of the common green bottle fly (lucilia sericata) produce antibacterial secretions that have a therapeutic effect on chronic and nonhealing wounds. recent developments in insect biotechnology have made it possible to use these larvae as a source of novel anti-infectives. here, we report the application of next-generation rna sequencing (rna-seq) to characterize the transcriptomes of the larval glands, crop, and gut, which contribute to the synthesis of antimicrobial peptides (amps) an ... | 2015 | 25666157 |
| bioluminescence based biosensors for quantitative detection of enterococcal peptide-pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection. | enterococcus faecalis is a significant threat in the nosocomial setting due to the emergence of isolates that are multi-antibiotic resistant, refractory to the available therapies and equipped with a variety of pathogenicity determinants. this bacterium uses quorum-sensing systems to regulate its physiological processes, including the expression of virulence traits, to adapt and proliferate within a host. here, we describe the construction and application of two bioluminescence-based reporter sy ... | 2015 | 25661457 |
| daptomycin versus vancomycin in an enterococcus faecalis endophthalmitis rabbit model. | to compare bactericidal activities of daptomycin (dap) and vancomycin (van) in an experimental rabbit model of enterococcus faecalis endophthalmitis. | 2016 | 25658242 |
| drinking water quality and public health in southwestern saudi arabia: the need for a national monitoring program. | the aim was to investigate the bacteriological quality of drinking water, and explore the factors involved in the knowledge of the public about the quality of drinking water in najran region, saudi arabia. | 2017 | 25657607 |
| sealing ability of a novel hydrophilic vs. conventional hydrophobic obturation systems: a bacterial leakage study. | comparative assessment of apical sealing ability of a novel smart-seal system, resilon, and conventional gutta-percha system using a bacterial leakage model. | 2017 | 25657530 |
| behavior of listeria monocytogenes in a multi-species biofilm with enterococcus faecalis and enterococcus faecium and control through sanitation procedures. | the formation of mono-species biofilm (listeria monocytogenes) and multi-species biofilms (enterococcus faecium, enterococcus faecalis, and l. monocytogenes) was evaluated. in addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. the biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39°c) and contact times (0, 1, 2, 4, 6 and 8 days). in all tests, at 7°c, the microbial counts were below 0.4 ... | 2015 | 25655573 |
| enterococcus faecalis attenuates the differentiation of macrophages into osteoclasts. | enterococcus faecalis is closely associated with refractory apical periodontitis, manifesting periapical lesions and alveolar bone loss. macrophages playing an important role in the induction of inflammation can differentiate into bone-resorbing osteoclasts. in the present study, we investigated the effect of e. faecalis on the differentiation and function of macrophages as osteoclast precursors. | 2015 | 25649294 |
| extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins. | sulfated polysaccharides were extracted from gray triggerfish (gtsp) and smooth hound (shsp) skins. their chemical and physical characteristics were determined using x-ray diffraction and infrared spectroscopic analysis. the antibacterial activities of gtsp and shsp against listeria monocytogenes (atcc 43251), staphylococcus aureus (atcc 25923), enterococcus faecalis (atcc 29212), escherichia coli (atcc 25922), salmonella enterica (atcc 43972) and enterobacter sp were evaluated by determining cl ... | 2015 | 25647621 |
| antibiofilm efficacy evaluation of a bioelectric dressing in mono- and multi-species biofilms. | chronic infections are often related to the formation of single or polymicrobial biofilms, which lead to hard-to-treat infections, difficult wound management, and recurrent infections. in this study, we evaluated in vitro antibiofilm properties of a silver and zinc bioelectric dressing (procellera). | 2015 | 25647504 |
| horizontal transfer of antibiotic resistance from enterococcus faecium of fermented meat origin to clinical isolates of e. faecium and enterococcus faecalis. | enterococcus species are part of the normal intestinal flora of a large number of mammals including humans and consequently, they can be used as indicators of faecal contamination in food and water for human consumption. their presence in large numbers in foods may indicate a lapse in sanitation and their ability to serve as a genetic reservoir of transferable antibiotic resistance is of concern. in the present study, enterococcus spp., isolated from commercially fermented meat and human clinica ... | 2015 | 25647243 |
| detection of vancomycin resistances in enterococci within 3 ½ hours. | vancomycin resistant enterococci (vre) constitute a challenging problem in health care institutions worldwide. novel methods to rapidly identify resistances are highly required to ensure an early start of tailored therapy and to prevent further spread of the bacteria. here, a spectroscopy-based rapid test is presented that reveals resistances of enterococci towards vancomycin within 3.5 hours. without any specific knowledge on the strain, vre can be recognized with high accuracy in two different ... | 2015 | 25645753 |
| β-lactam combinations with daptomycin provide synergy against vancomycin-resistant enterococcus faecalis and enterococcus faecium. | enterococcus faecalis (efc) and enterococcus faecium (efm) are frequently resistant to vancomycin and β-lactams (bls). in vitro data suggest synergy between several bls and glycopeptides or lipopeptides against resistant pathogens. our objective was to conduct combination mic and time-kill experiments to evaluate bl synergy with daptomycin against enterococci. | 2015 | 25645208 |
| antimicrobial activity of different disinfection methods against biofilms in root canals. | the aim of the present study was to evaluate the bactericidal effects of different disinfection methods against microbial biofilms grown in root canals. | 2016 | 25641829 |
| enterococcus faecalis with capsule polysaccharides type 2 and biofilm-forming capacity in indonesians requiring endodontic treatment. | the aim of the present study was to evaluate the genetic diversity of enterococcus faecalis (e. faecalis) strains isolated from saliva and infected root canal samples from indonesians requiring endodontic treatment. | 2015 | 25641820 |
| diagnostic and therapeutic utility of transrectal ultrasound in urological office prostatic abscess management: a short report from a single urologic center. | prostatic abscess (pa) is an infrequent condition in the modern antibiotic era. the everyday use of transrectal ultrasound (trus) during diagnostic work-up and the widespread recurrence to prostatic biopsies may lead to an increase of pa diagnosis. in this short report we analyze the patients characteristics and the management of seven recent cases of pa diagnosed in our institution. | 2014 | 25641468 |
| natural sesquiterpene lactones enhance oxacillin and gentamicin effectiveness against pathogenic bacteria without antibacterial effects on beneficial lactobacilli. | this is a report on the synergistic interactions (sis) between melampolide-type sesquiterpene lactones 1-8 from acanthospermum hispidum dc., and oxacillin or gentamicin, against four pathogenic strains of staphylococcus aureus and enterococcus faecalis; two of them were multi-resistant strains obtained from chronic infectious processes. our results showed that all associations of 1-8 with antibiotics (atbs) are more effective than pure atbs to control pathogenic strains of s. aureus and e. faeca ... | 2015 | 25641272 |
| antibacterial effect of butyryl alkannin from arnebia euchroma against vancomycin-resistant pathogens of enterococcus faecalis causing urinary tract infections. | this study was carried out to investigate the biomedicinal potential of a bioactive marker component, butyryl alkannin, isolated from n-hexane root extract of arnebia euchroma against various vancomycin-resistant enterococcus (vre) isolates of enterococcus faecalis causing urinary tract infections. as a result, butyryl alkannin showed significant antibacterial activity against multidrug-resistant e. faecalis pathogens of vre as minimum inhibitory concentration values which were found in the rang ... | 2015 | 25635653 |
| ef0176 and ef0177 from enterococcus faecalis v583 are substrate-binding lipoproteins involved in abc transporter mediated ribonucleoside uptake. | one of the abc transporter systems in enterococcus faecalis v583 is encoded by the ef0176-ef0180 gene cluster, which differs from orthologous operons in related bacteria in that it contains two genes putatively encoding substrate-binding proteins (sbps). these sbps, ef0176 and ef0177, have previously been identified on the surface of e. faecalis. by phenotypic studies of single and double knockout mutants, we show here that ef0176 and ef0177 are specific for ribonucleosides and, by inference, th ... | 2015 | 25635276 |
| in vitro activity of linezolid as assessed through the 2013 leader surveillance program. | the 2013 leader surveillance program monitored the in vitro activity of linezolid and comparator agents against gram-positive bacteria at 60 medical centers in the united states. a total of 7183 pathogens were contributed from 6 predetermined pathogen groups. the groups were staphylococcus aureus, coagulase-negative staphylococci, enterococci, streptococcus pneumoniae, β-hemolytic streptococci, and viridans group streptococci. the mic90 value for each of the 6 pathogen groups was 1 μg/ml. suscep ... | 2015 | 25633420 |
| antimicrobial activity of extracts and isoquinoline alkaloids of selected papaveraceae plants. | alkaloidal extracts of seven selected plants of the family papaveraceae were studied with respect to their activity against six strains of pathogenic bacteria and their alkaloidal fingerprint. twenty-four alkaloids were determined by gc/ms, and twenty of them identified from their mass spectra, retention times and retention indexes. in the antibacterial assay, three gram-positive (enterorococcus faecalis, staphylococcus aureus and s. hyicus), and three gram-negative (escherichia coli, proteus mi ... | 2014 | 25632464 |
| efficacy of contemporary and novel intracanal medicaments against enterococcus faecalis. | to compare the antibacterial activity of propolis (30% in methyl cellulose), curcumin (2.5mg/ml of methyl cellulose), 2% chlorhexidine gel (chx), 2% metronidazole gel (mz) and a mixture of 2% chx and 2% mz against enterococcus faecalis in vitro. calcium hydroxide served as the control. | 2014 | 25631726 |
| an in vivo comparison of antimicrobial efficacy of sodium hypochlorite and biopure mtad™ against enterococcus faecalis in primary teeth: a qpcr study. | biopure mtad™, a new root canal irrigant has shown promising results against the most common resistant microorganism, e. faecalis, in permanent teeth. however, there is lack of studies comparing its antimicrobial effectiveness with naocl in primary teeth. the purpose of this study was to compare the in vivo antimicrobial efficacy of naocl 2.5% and biopure mtad™ against e. faecalis in primary teeth. | 2014 | 25631723 |
| ex vivo evaluation of three instrumentation techniques on e. faecalis biofilm within oval shaped root canals. | the objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with enterococcus faecalis. forty-five human lower premolars were infected with a culture of e. faecalis (atcc 29212) for 28 days. five other teeth that were neither contaminated nor instrumented were used as controls. the 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (k- ... | 2015 | 25627890 |
| update of the telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2013), when applying the revised susceptibility testing method. | a revised broth microdilution susceptibility testing method for telavancin was approved by the food and drug administration (fda). telavancin activity was assessed against gram-positive pathogens collected worldwide (2013) using the revised method. a total of 12,346 isolates from 90 sites were included as part of the telavancin international surveillance program for the americas, europe, and asia-pacific. telavancin had mic50 and mic90 values of 0.03 and 0.06 μg/ml, respectively, against staphyl ... | 2015 | 25618421 |
| dietary enterococcus faecalis lab31 improves growth performance, reduces diarrhea, and increases fecal lactobacillus number of weaned piglets. | lactic acid bacteria (lab) have been shown to enhance performance of weaned piglets. however, few studies have reported the addition of lab enterococcus faecalis as alternatives to growth promoting antibiotics for weaned piglets. this study evaluated the effects of dietary e. faecalis lab31 on the growth performance, diarrhea incidence, blood parameters, fecal bacterial and lactobacillus communities in weaned piglets. a total of 360 piglets weaned at 26 ± 2 days of age were randomly allotted to ... | 2015 | 25617897 |
| synthesis of silver nanoparticles using solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line mcf 7. | in the present study, we have synthesized silver nanoparticles by a simple and eco-friendly method using unripe fruits of solanum trilobatum. the aqueous silver ions when exposed to unripe fruits extract were reduced and stabilized over long time resulting in biosynthesis of surface functionalized silver nanoparticles. the bio-reduced silver nanoparticles were characterized by uv-visible spectroscopy, fourier transform infrared spectroscopy (ftir), scanning electron microscopy (sem), transmissio ... | 2015 | 25613692 |
| gold nanoshell-decorated silicone surfaces for the near-infrared (nir) photothermal destruction of the pathogenic bacterium e. faecalis. | catheter-related infections (cris) are associated with the formation of pathogenic biofilms on the surfaces of silicone catheters, which are ubiquitous in medicine. these biofilms provide protection against antimicrobial agents and facilitate the development of bacterial resistance to antibiotics. the application of photothermal agents on catheter surfaces is an innovative approach to overcoming biofilm-generated cris. gold nanoshells (aunss) represent a promising photothermal tool, because they ... | 2015 | 25611157 |
| antibiotic resistance prevalence in routine bloodstream isolates from children's hospitals varies substantially from adult surveillance data in europe. | surveillance of antimicrobial resistance (amr) is central for defining appropriate strategies to deal with changing amr levels. it is unclear whether childhood amr patterns differ from those detected in isolates from adult patients. | 2015 | 25607829 |
| fluoride varnish as root canal sealer: a scanning electron microscopy and bacterial penetration study. | this study was carried out to evaluate the bacterial leakage of root canal fillings when cavity varnish containing 5% fluoride (duraflur) was used as root canal sealer. | 2015 | 25598813 |
| antimicrobial activity of nanoparticle calcium hydroxide against enterococcus faecalis: an in vitro study. | enterococcus faecalis (e. faecalis) has the ability to invade the dentinal tubules and resist high ph levels. as a result, calcium hydroxide (ch) is not much effective on this bacterium. in theory, nanoparticle calcium hydroxide (nch) has smaller size and high surface area that enables it to penetrate into the deeper layers of dentin and be more effective on e. faecalis. this in vitro study was designed to compare the antimicrobial activity of nch and ch against e. faecalis. | 2015 | 25598808 |
| efficacy of 3d conforming nickel titanium rotary instruments in eliminating canal wall bacteria from oval-shaped root canals. | to evaluate the effectiveness of trushape® 3d conforming files, compared with twisted files, in reducing bacteria load from root canal walls, in the presence or absence of irrigant agitation. | 2015 | 25593001 |
| manual and rotary instrumentation ability to reduce enterococcus faecalis associated with photodynamic therapy in deciduous molars. | this aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (pdt) to reduce enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue o/laser, fuchsin/halogen light and fuchsin/led. twenty deciduous molars were selected and contaminated with enterococcus faecalis (mcfarland 0.5 scale). working length determination was performed by visual method. the teeth were randomly divided into two groups: g1 (n=10): ... | 2016 | 25590196 |
| different vana elements in e. faecalis and in e. faecium suggest at least two origins of tn1546 among vre in a brazilian hospital. | in 2009 during surveillance in a brazilian hospital, many patients were confirmed to be colonized by vancomycin-resistant enterococci (vre) and a few infection cases occurred. among 14 isolates of enterococcus faecalis, most had the same pulsotype, virulence profile (agg(+)elra(+)gele(+)), and were sequence type (st)103, indicating dissemination of a clone. the 47 enterococcus faecium were separated into four pulsotypes, the predominant virulence profile being esp(+)acm(+). all of them harbored ... | 2015 | 25588068 |
| antibacterial effect of all-in-one self-etch adhesives on enterococcus faecalis. | background and aims. the aim of this study was to evaluate the antibacterial activity of one-step self-etch adhesives on enterococcus faecalis on days 1, 7 and 14 with the use of modified direct contact test. materials and methods. the modified directcontact test was used to evaluate the antibacterial effect of adper easy one, bond force, clearfil s3 bond, futurabond m, g-bond, ibond and optibond all-in-one adhesives on enterococcus faecalis after aging the samples in phosphate-buffered saline f ... | 2014 | 25587384 |
| assessment of photodynamic therapy (pdt) in disinfection of deeper dentinal tubules in a root canal system: an in vitro study. | the success of endodontic treatment therapy depends on how well we eliminate pathogenic microflora from the root canal system as micro organism as the major cause of root canal infection. conventional root canal treatment can fail if microorganisms cannot be removed sufficiently by thorough cleaning, shaping of root canal. newer modalities such as photodynamic therapy are being tried now a days for disinfection of root canals. aim & objectives: the basic aim of this study was assessment of the a ... | 2014 | 25584321 |
| an in vitro evaluation of antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin. | to evaluate in vitro the antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin. | 2014 | 25584319 |
| characterisation of the efficacy of endodontic medications using a three-dimensional fluorescent tooth model: an ex vivo study. | the purpose of this study was to establish a three-dimensional fluorescent tooth model to investigate bacterial viability against intra-canal medicaments across the thickness and surface of root dentine. dental microbial biofilms (enterococcus faecalis and streptococcus mutans) were established on the external root surface and bacterial kill was monitored over time against intra-canal medicament (ca(oh)2 ) using fluorescent microscopy in conjunction with baclight syto9 and propidium iodide stain ... | 2015 | 25583457 |
| l-lactate production from biodiesel-derived crude glycerol by metabolically engineered enterococcus faecalis: cytotoxic evaluation of biodiesel waste and development of a glycerol-inducible gene expression system. | biodiesel waste is a by-product of the biodiesel production process that contains a large amount of crude glycerol. to reuse the crude glycerol, a novel bioconversion process using enterococcus faecalis was developed through physiological studies. the e. faecalis strain w11 could use biodiesel waste as a carbon source, although cell growth was significantly inhibited by the oil component in the biodiesel waste, which decreased the cellular nadh/nad(+) ratio and then induced oxidative stress to c ... | 2015 | 25576618 |
| the new schiff base 4-[(4-hydroxy-3-fluoro-5-methoxy-benzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydro-pyrazol-3-one: experimental, dft calculational studies and in vitro antimicrobial activity. | the synthesized schiff base, 4-[(4-hydroxy-3-fluoro-5-methoxy-benzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydro-pyrazol-3-one (i), has been characterized by (13)c nmr, (1)h nmr, 2d nmr ((1)h-(1)h cosy and (13)c apt), ft-ir, uv-vis and x-ray single-crystal techniques. molecular geometry of the compound i in the ground state, vibrational frequencies and chemical shift values have been calculated by using the density functional method (dft) with 6-311++g(d,p) basis set. the obtained results indi ... | 2015 | 25574656 |
| risk factors analysis for occurrence of asymptomatic bacteriuria after endourological procedures. | endourological procedures are performed according to the principles of aseptic techniques, jet still in certain number of patients urinary tract infections may occur. considering the risk of urinary tract infection, there is no unique opinion about the prophylactic use of antibiotics in endourological procedures. | 2014 | 25568546 |
| the catastrophic journey of a retained temporary epicardial pacemaker wire leading to enterococcus faecalis endocarditis and subsequent stroke. | a 52-year-old man underwent triple coronary artery bypass graft (cabg). three months later, he presented to the emergency room with dizziness and left-sided weakness. workup revealed right frontal parenchymal haemorrhage; also, two retained temporary epicardial pacemaker wires (tepw) from his previous cabg were observed, one of which had significantly migrated. one wire was found in the soft tissues below the heart; the other transversed the mediastinum from the expected location of the right at ... | 2015 | 25568268 |
| susceptibility of glycopeptide-resistant enterococci to linezolid, quinupristin/dalfopristin, tigecycline and daptomycin in a tertiary greek hospital. | we investigated the antibiotic susceptibility of glycopeptide-resistant enterococci (gre). seventy consecutive gre were tested. sixty-two isolates were identified as enterococcus faecium (88.6%), and 8 (11.4%) as enterococcus faecalis. all strains were susceptible to linezolid and daptomycin, while 17.1% (12/70) and 11.4% (8/70) were resistant to quinupristin/dalfopristin (qd) and tigecycline, respectively. all e. faecalis isolates were resistant to qd, while 4 of 62 (6.5%) e. faecium isolates w ... | 2014 | 25566405 |
| nanobioarchitectures based on chlorophyll photopigment, artificial lipid bilayers and carbon nanotubes. | in the last decade, building biohybrid materials has gained considerable interest in the field of nanotechnology. this paper describes an original design for bionanoarchitectures with interesting properties and potential bioapplications. multilamellar lipid vesicles (obtained by hydration of a dipalmitoyl phosphatidylcholine thin film) with and without cholesterol were labelled with a natural photopigment (chlorophyll a), which functioned as a sensor to detect modifications in the artificial lip ... | 2014 | 25551059 |
| antimicrobial activity of prodigiosin isolated from serratia marcescens ufpeda 398. | prodigiosin is an alkaloid and natural red pigment produced by serratia marcescens. prodigiosin has antimicrobial, antimalarial and antitumor properties and induces apoptosis in t and b lymphocytes. these properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries. the aim of the present study was to evaluate the antimicrobial activity of prodigiosin against pathogenic micro-organisms. the red pigments produced by s. marcescens exhibited a ... | 2015 | 25549906 |
| bactericidal efficacy of cold plasma at different depths of infected root canals in vitro. | cold plasma (cp) has been shown to be effective even against multiresistant microorganisms. as previous investigations on the effect of cp in root canals showed promising results, the aim of the present study was to analyze the bactericidal efficacy of cp in different depths of infected dentin. | 2015 | 26962378 |
| detection of listeria monocytogenes with short peptide fragments from class iia bacteriocins as recognition elements. | we employed a direct peptide-bacteria binding assay to screen peptide fragments for high and specific binding to listeria monocytogenes. peptides were screened from a peptide array library synthesized on cellulose membrane. twenty four peptide fragments (each a 14-mer) were derived from three potent anti-listerial peptides, leucocin a, pediocin pa1, and curvacin a, that belong to class iia bacteriocins. fragment leu10 (geafsagvhrlang), derived from the c-terminal region of leucocin a, displayed ... | 2015 | 25548942 |
| population biology of intestinal enterococcus isolates from hospitalized and nonhospitalized individuals in different age groups. | the diversity of enterococcal populations from fecal samples from hospitalized (n = 133) and nonhospitalized individuals (n = 173) of different age groups (group i, ages 0 to 19 years; group ii, ages 20 to 59 years; group iii, ages ≥60 years) was analyzed. enterococci were recovered at similar rates from hospitalized and nonhospitalized persons (77.44% to 79.77%) of all age groups (75.0% to 82.61%). enterococcus faecalis and enterococcus faecium were predominant, although seven other enterococcu ... | 2015 | 25548052 |
| transcriptome analysis of enterococcus faecalis during mammalian infection shows cells undergo adaptation and exist in a stringent response state. | as both a commensal and a major cause of healthcare-associated infections in humans, enterococcus faecalis is a remarkably adaptable organism. we investigated how e. faecalis adapts in a mammalian host as a pathogen by characterizing changes in the transcriptome during infection in a rabbit model of subdermal abscess formation using transcriptional microarrays. the microarray experiments detected 222 and 291 differentially regulated genes in e. faecalis og1rf at two and eight hours after subderm ... | 2014 | 25545155 |
| tyramine biosynthesis is transcriptionally induced at low ph and improves the fitness of enterococcus faecalis in acidic environments. | enterococcus faecalis is a commensal bacterium of the human gut that requires the ability to pass through the stomach and therefore cope with low ph. e. faecalis has also been identified as one of the major tyramine producers in fermented food products, where they also encounter acidic environments. in the present work, we have constructed a non-tyramine-producing mutant to study the role of the tyramine biosynthetic pathway, which converts tyrosine to tyramine via amino acid decarboxylation. wi ... | 2015 | 25529314 |
| using a genome-scale metabolic model of enterococcus faecalis v583 to assess amino acid uptake and its impact on central metabolism. | increasing antibiotic resistance in pathogenic bacteria necessitates the development of new medication strategies. interfering with the metabolic network of the pathogen can provide novel drug targets but simultaneously requires a deeper and more detailed organism-specific understanding of the metabolism, which is often surprisingly sparse. in light of this, we reconstructed a genome-scale metabolic model of the pathogen enterococcus faecalis v583. the manually curated metabolic network comprise ... | 2015 | 25527553 |
| effects of epigallocatechin gallate against enterococcus faecalis biofilm and virulence. | epigallocatechin-3-gallate (egcg), the most abundant polyphenol in green tea (camellia sinesis) has been shown to exert antimicrobial effects on numerous bacterial pathogens. however its efficacy against enterococcus faecalis biofilm, which is associated with persistent root canal infection is unknown. the aims of this study were to investigate the effects of egcg against e. faecalis biofilm and virulence. | 2015 | 25526623 |