| aortic homograft endocarditis caused by campylobacter jejuni. | we report the first case of homograft endocarditis caused by campylobacter jejuni, which was treated successfully with antibiotic therapy and valve replacement. to our knowledge, only two other cases of c. jejuni endocarditis, involving native valves, have been reported in the medical literature. | 2011 | 21940472 |
| occurrence of the invasion associated marker (iam) in campylobacter jejuni isolated from cattle. | abstract: background: the invasion associated marker (iam) has been detected in the majority of invasive campylobacter jejuni retrieved from humans. furthermore, the detection of iam in c. jejuni isolated from two important hosts, humans and chickens, suggested a role for this marker in c. jejuni's colonization of multiple hosts. however, no data exist regarding the occurrence of this marker in c. jejuni isolated from non-poultry food-animals such as cattle, an increasingly important source for ... | 2011 | 22208406 |
| effects of polyphosphate additives on the ph of processed chicken exudates and the survival of campylobacter. | campylobacter spp. are nutritionally fastidious organisms that are sensitive to normal atmospheric oxygen levels and lack homologues of common cold shock genes. at first glance, these bacteria seem ill equipped to persist within food products under processing and storage conditions; however, they survive in numbers sufficient to cause the largest number of foodborne bacterial disease annually. a mechanism proposed to play a role in campylobacter survival is the addition of polyphosphate-contain ... | 2011 | 22004823 |
| guillain-barré syndrome associated with normal or exaggerated tendon reflexes. | areflexia is part one of the clinical criteria required to make a diagnosis of guillain-barré syndrome (gbs). the diagnostic criteria were stringently developed to exclude non-gbs cases but there have been reports of patients with gbs following campylobacter jejuni enteritis with normal and exaggerated deep tendon reflexes (dtrs). the aim of this study is to expand the existing diagnostic criteria to preserved dtrs. from the cohort of patients referred for anti-ganglioside antibody testing from ... | 2011 | 22143612 |
| application of high-throughput sequencing to measure the performance of commonly used selective cultivation methods for the foodborne pathogen campylobacter. | campylobacter is an important foodborne human pathogen, which has traditionally been studied using a variety of selective cultivation methods. here we use next-generation sequencing to ask the following: (i) how selective are commonly used campylobacter cultivation methods relative to the initial sample and (ii) how do the specificity and sensitivity of these methods compare with one another? to answer these questions, we used 16s rrna tagged-pyrosequencing to sequence directly from a pooled fec ... | 2011 | 22092388 |
| the microbiological quality of beef tripe using different processing techniques. | the microbiological quality of routinely processed tripe and rumen pillars were compared with those derived after emptying the rumen (paunch) without using water (dry dumping) and after deliberately bursting the paunches before processing. prior to packing the mean log(10) aerobic plate counts (apc) for the routinely processed tripe and rumen pillars were 3.55±1.08 and 3.28±0.87/g respectively. the corresponding mean log(10) total coliform counts (tcc) were 1.27±1.28 and 2.08±0.87. the mean log ... | 2002 | 22061196 |
| molecular evidence for zoonotic transmission of an emergent highly pathogenic campylobacter jejuni clone in the united states. | campylobacter jejuni is a major zoonotic pathogen. a highly virulent, tetracycline-resistant c. jejuni clone (named clone sa) has recently emerged in ruminant reservoirs and become the predominant cause of sheep abortion in the u.s. to determine whether clone sa is associated with human disease, we compared the clinical isolates of clone sa from sheep abortions with the human isolates of the pulsenet national campylobacter databases at cdc and fda using pulsed field gel electrophoresis (pfge), m ... | 2011 | 22189122 |
| bacteriological safety issues in red meat and ready-to-eat meat products, as well as control measures. | the importance of eschericha coli o157, listeria monocytogenes and salmonella typhimurium dt104 as meat-borne pathogens is well established. pathogenic bacteria such as aeromonas spp., arcobacter spp., psychrotrophic bacillus cereus, campylobacter spp., clostridium botulinum and non-invasive listeria monocytogenes can be regarded as rookies, but not yet firmly associated with today's production of red meat and meat products. the development of pcr and other dna-based techniques will shed new lig ... | 2002 | 22061614 |
| the subgingival microbiota of papillon-lefèvre syndrome. | background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ... | 2011 | 22141356 |
| fate and survival of campylobacter coli in swine manure at various temperatures. | campylobacter coli is the most common campylobacter species found in pig (95%), but the ability of this bacterium to survive in swine manure as well as the potential for causing human illness are poorly understood. we present here laboratory-scale experiments to investigate the effect of temperature on the survival of c. coli in spiked swine manure samples at temperatures from 4 to 52°c. the survival of c. coli during storage for 30 days was studied by three different methods: bacterial culture ... | 2011 | 22207868 |
| estimating the burden of acute gastroenteritis and foodborne illness caused by campylobacter, salmonella, and vibrio parahaemolyticus by using population-based telephone survey data, miyagi prefecture, japan, 2005 to 2006. | most cases of acute gastroenteritis and foodborne disease are not ascertained by public health surveillance because the ill person does not always seek medical care and submit a stool sample for testing, and the laboratory does not always test for or identify the causative organism. we estimated the total burden of acute gastroenteritis in miyagi prefecture, japan, using data from two 2-week cross-sectional, population-based telephone surveys conducted in 2006 and 2007. to estimate the number o ... | 2011 | 22004803 |
| Inaccuracy of the Disk Diffusion Method Compared with the Agar Dilution Method for Susceptibility Testing of Campylobacter spp. | The agar dilution method has been standardized by the CLSI for the susceptibility testing of Campylobacter species, and according to these standards, the disk diffusion method should be used only in screening for macrolide and ciprofloxacin resistance. Nevertheless, the disk diffusion test is currently widely used, since it is easy to perform in clinical microbiology laboratories. In this study, the disk diffusion method was compared to the agar dilution method by analyzing the in vitro activiti ... | 2012 | 22075583 |
| [quinolone resistant campylobacter jejuni strains isolated from humans and from poultry]. | eight quinolone resistant campylobacter jejuni strains isolated from humans with diarrheal disease were compared with 23 isolates from chicken and from laying hens. samples were cultured on selective agar in microaerophilia, identified by conventional tests, and conserved in 17% glycerol at -70 c. clones were determined by rapd-pcr employing the 1254 primer (stern nj). five patterns were obtained. patterns i, ii, and v were found in both poultry and human isolates. pattern i was obtained from po ... | 2011 | 21893445 |
| cmer-dependent gene cj0561c is induced more effectively by bile salts than the cmeabc efflux pump in both human and poultry campylobacter jejuni strains. | the multidrug efflux pump cmeabc described in the food-borne pathogen campylobacter jejuni was shown to be an important element of bile resistance and significant for successful colonization of chicken intestines. recently, another gene (cj0561c) strongly suppressed by the same repressor (cmer) that regulates expression of cmeabc was identified in c. jejuni nctc 11168. initial data suggested that, similarly to cmeabc, cj0561c could be induced by bile salts. in the present study, the occurrence o ... | 2011 | 21920435 |
| the critical role of luxs in the virulence of campylobacter jejuni in a guinea pig model of abortion. | previous studies on campylobacter jejuni have demonstrated the role of luxs in motility, cytolethal distending toxin production, agglutination and intestinal colonization, however its direct involvement in virulence has not been reported. in this study we demonstrate a direct role of luxs in the virulence of c. jejuni in two different animal hosts. the ia3902 strain, a highly virulent sheep abortion strain recently described by our laboratory, along with its isogenic luxs mutant and luxs complem ... | 2011 | 22144479 |
| closely related campylobacter jejuni strains from different sources reveal a generalist rather than a specialist lifestyle. | abstract: background: campylobacter jejuni and campylobacter coli are human intestinal pathogens of global importance. zoonotic transmission from livestock animals or animal-derived food is the likely cause for most of these infections. however, little is known about their general and host-specific mechanisms of colonization, or virulence and pathogenicity factors. in certain hosts, campylobacter species colonize persistently and do not cause disease, while they cause acute intestinal disease i ... | 2011 | 22122991 |
| enrichment culture can bias the isolation of campylobacter subtypes. | summaryenrichment culture is often used to isolate campylobacter. this study compared isolation of campylobacter spp. from 119 broiler chicken environments from two farms, using preston and modified exeter (mexeter) and modified bolton (mbolton) enrichments. mexeter was significantly more effective in isolating campylobacter spp. from the environmental samples compared to preston (p<0·001) and mbolton (p<0·04) broths but there was no significant difference between the latter two methods (p>0·05) ... | 2011 | 21923970 |
| immunopathology and th1/th2 immune response of campylobacter jejuni-induced paralysis resembling guillain-barré syndrome in chicken. | immunopathogenesis of campylobacter jejuni-associated guillain-barré syndrome (gbs) is not yet well established probably due to lack of experimental model. therefore, we studied the th1/th2 immune response and pathological changes in c. jejuni-induced chicken model for gbs. c. jejuni (5 × 10(9) cfu/ml) and placebo were fed to 30 chickens each. stools of all birds were negative for c. jejuni by culture and pcr before experiment. the birds were regularly assessed for disease symptoms up to 30 days ... | 2011 | 22102098 |
| pathogens on meat and infection in animals - establishing a relationship using campylobacter and salmonella as examples. | a high proportion of human campylobacter and salmonella infections is likely to originate from farm animals, usually directly from the consumption of contaminated meat or milk. surveillance shows that campylobacter and salmonella genotypes are shared between human case isolates, farm animals and foods, although with the latter there can be marked differences between infection frequency in live animals and contamination rates in raw foods. this is supported by a variety of data from around the wo ... | 2006 | 22062719 |
| phylogenetic analysis of urease-positive thermophilic campylobacter (uptc) strains based on the molecular characterization of the flaa gene. | molecular cloning, nucleotide sequencing, and characterization of the flaa gene from additional isolates of urease-positive thermophilic campylobacter (uptc) were performed. these isolates were obtained from the natural environment in northern ireland (n = 9 from mussels) and in england (n = 1 from sea water). all isolates carried the shorter flaa gene, [open reading frames (orfs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaa pseudogenes. the up ... | 2011 | 21874596 |
| campylobacter spp. distribution in biofilms on different surfaces in an agricultural watershed (elk creek, british columbia): using biofilms to monitor for campylobacter. | despite its relevance to public health, presence and concentrations of campylobacter spp. in biofilms in natural aquatic environments has not been investigated. this study examined the occurrence of campylobacter spp. in biofilms on a variety of surfaces (river rock, slate rock, wood, lexan™, sandpaper, and sediment) and in water from december 2005 to december 2006 to find a substratum that facilitated campylobacters detection in natural aquatic environments. samples were collected at four sites ... | 2011 | 22204983 |
| effect of a self-etching adhesive containing an antibacterial monomer on clinical periodontal parameters and subgingival microbiologic composition in orthodontic patients. | the aims of this study were to evaluate the effect of a self-etching adhesive system containing an antibacterial monomer on periodontal health and subgingival microbiologic composition in orthodontic patients and to compare it with a conventional adhesive system. | 2011 | 21967952 |
| phsysiological characterization of campylobacter jejuni under cold stresses conditions: its potential for public threat. | campylobacter jejuni is the major cause of human gastroenteritis worldwide. under stress conditions, c. jejuni can enter a viable but non-culturable (vbnc) state. we found that the c. jejuni was able to enter a vbnc state by prolonged incubation at 4℃. the standard isolation methods using pre-enrichment steps in bolton broth or preston broth could not detect the vbnc cells in spiked chicken meat. the transcription levels of virulence-associated genes (flaa, flab, cadf, ciab, cdta, cdtb and cdtc) ... | 2011 | 21891974 |
| real-time multiplex pcr for simultaneous detection of campylobacter jejuni, salmonella, shigella and yersinia species in fecal samples. | diarrheal diseases due to notifiable bacterial infections require rapid diagnosis of the causative pathogens. to facilitate detection, a real-time multiplex pcr was developed that identifies common diarrhea-causing bacteria in fecal samples. on the basis of published sequence data, sets of primers and probes were designed that were specific for campylobacter jejuni, salmonella, shigella/enteroinvasive escherichia coli eiec, and yersinia species, suitable for use in a one-tube pcr assay. the assa ... | 2011 | 21855409 |
| a specificity determinant for phosphorylation in a response regulator prevents in vivo cross-talk and modification by acetyl phosphate. | bacterial two-component systems (tcss) sense stimuli and transduce signals intracellularly through phosphotransfer between cognate histidine kinases (hks) and response regulators (rrs) to alter gene expression or behavioral responses. without high phosphotransfer specificity between cognate hks and rrs, cross-phosphorylation or cross-talk between different tcss may occur and diminish responses to appropriate stimuli. some mechanisms to reduce cross-talk involve hks controlling levels of cognate ... | 2011 | 22128335 |
| £4 million funding for research on campylobacter. | | 2011 | 21926134 |
| Campylobacter jejuni Induces Colitis Through Activation of Mammalian Target of Rapamycin Signaling. | Campylobacter jejuni is the worldwide leading cause of bacterial-induced enteritis. The molecular and cellular events that lead to campylobacteriosis are poorly understood. We identify mammalian target of rapamycin (mTOR) as a signaling pathway that leads to C jejuni-induced intestinal inflammation. | 2012 | 21963787 |
| sequencing of cjie1 prophages from campylobacter jejuni isolates reveals the presence of inserted and (or) deleted genes. | bacteriophages capable of integrating into host bacterial genomes as prophages affect the biology and virulence of their bacterial hosts. previously, partial sequencing of 12 prophages similar to cjie1 from campylobacter jejuni rm1221 did not show the presence of inserted nonphage genes. therefore, four of these prophages were sequenced completely, and indels were found in at least two different regions of the prophage genome. putative proteins from one indel appeared to be members of two new fa ... | 2011 | 21939376 |
| [detection of antibodies to campylobacter jejuni in pediatrics patients with gullain-barré syndrome using different antigen preparations]. | diagnosis of previous c. jejuni infections in gbs patients are mostly based on serological findings. however, there are not consensus what kind of antigen should be used in the serological assays. in our study we used elisa with four different antigen preparations for investigation of specific antibodies to c. jejuni in serum samples obtained from 6 children with gbs. in all patients the high level of iga and igg antibodies to lps were diagnosed. the antibodies in particular classes of immunoglo ... | 2011 | 22184920 |
| occurrence of putative virulence genes in arcobacter species isolated from humans and animals. | interest for arcobacters in veterinary and human public health has increased since the first report of the isolation of arcobacters from food of animal origin. since then, studies worldwide have reported the occurrence of arcobacters on food and in food-production animals and have highlighted the possible transmission of especially a. butzleri to the human population. in humans, arcobacters are associated with enteritis and septicemia. to assess their clinical relevance for humans and animals, e ... | 2011 | 22170914 |
| contribution of tat system translocated phox to campylobacter jejuni phosphate metabolism and resilience to environmental stresses. | campylobacter jejuni is a common gastrointestinal pathogen that colonizes food animals; it is transmitted via fecal contamination of food, and infections in immune-compromised people are more likely to result in serious long-term illness. environmental phosphate is likely an important sensor of environmental fitness and the ability to obtain extracellular phosphate is central to the bacteria's core metabolic responses. phox is the sole alkaline phosphatase in c. jejuni, a substrate of the tat tr ... | 2011 | 22028859 |
| development and stability of bacteriocin resistance in campylobacter spp. | several bacteriocins (bcns) that were identified from chicken commensal bacteria dramatically reduced campylobacter colonization in poultry and are being directed toward on-farm control of this important foodborne human pathogen. a recent study has shown that bcn resistance in campylobacter jejuni is very difficult to develop in vitro. in this study, in vivo development and stability of bcn resistance in campylobacter was examined. | 2011 | 21973216 |
| fate of pathogens in a simulated bioreduction system for livestock carcasses. | the eu animal by-products regulations generated the need for novel methods of storage and disposal of dead livestock. bioreduction prior to rendering or incineration has been proposed as a practical and potentially cost-effective method; however, its biosecurity characteristics need to be elucidated. to address this, salmonella enterica (serovars senftenberg and poona), enterococcus faecalis, campylobacter jejuni, campylobacter coli and a lux-marked strain of escherichia coli o157 were inoculate ... | 2011 | 22119516 |
| Characterization of mono- and mixed-culture Campylobacter jejuni biofilms. | Campylobacter jejuni, one of the most common causes of human gastroenteritis, is a thermophilic and microaerophilic bacterium. These characteristics make it a fastidious organism, which limits its ability to survive outside of animal hosts. Nevertheless, C. jejuni can be transmitted to both humans and animals via environmental pathways, especially through contaminated water. Biofilms may play a crucial role in the survival of the bacterium under unfavorable environmental conditions. The goal of ... | 2011 | 22179238 |
| barrett's esophagus: proton pump inhibitors and chemoprevention i. | the following on proton pump inhibitors and chemoprevention in barrett's esophagus includes commentaries on normalization of esophageal refluxate; the effects of 5-ht(4) agonists on egf secretion and of lubripristone on chloride channels agents; the role of campylobacter toxin production; the deleterious effects of unconjugated bile acids; the role of baclofen in nonacid reflux; the threshold for adequate esophageal acid exposure; the effects of proton pump inhibitor (ppi) therapy on normalizat ... | 2011 | 21950809 |
| identification of lactobacilli residing in chicken ceca with antagonism against campylobacter. | bacteriocins produced by lactobacillus salivarius have been recently recognized as a natural means to control campylobacter and salmonella in live poultry. this finding is of relevance since campylobacter jejuni and campylobacter coli are the predominant species isolated from poultry that are associated with human campylobacteriosis. in the present work, lactic acid bacteria (lab) isolated from the cecum of twenty tunisian chickens were identified and those isolates with antagonism against campy ... | 2011 | 22069154 |
| Microbiological quality of water immersion-chilled and air-chilled broilers. | Carcass chilling during broiler processing is a critical step in preventing growth of pathogenic and spoilage bacteria. The objective of this study was to compare the microbiological quality of air- and water-chilled broiler carcasses processed at the same commercial facility. For each of four replications, 15 broilers were collected from the same commercial processing line after evisceration, after spraying with cetylpyridinium chloride (a cationic disinfectant), and after air chilling or water ... | 2011 | 21902923 |
| campylobacter contamination in broiler carcasses and correlation with slaughterhouses operational hygiene inspection. | this study investigates factors associated with campylobacter contamination of broiler carcasses, using survey data collected from nine belgian slaughterhouses in 2008 in accordance with a european union baseline study. campylobacter were detected in 51.9% (202/389) (95% confidence interval, 46.8%-56.9%) of broiler carcasses. campylobacter concentration was <10 cfu/g in 49.6% of carcasses, while 20.6% were contaminated with ≥ 1000 cfu/g. the mean campylobacter concentration, as calculated by max ... | 2012 | 22029924 |
| Clinical spectrum of serious bacterial infections among splenectomized patients with hemoglobinopathies in Israel: a 37-year follow-up study. | PURPOSE: Patients with hemoglobinopathies who undergo splenectomy are at risk for invasive infections. The aim of this investigation was to present the clinical spectrum of infections in splenectomized patients. METHODS: The study cohort comprised 54 splenectomized patients with beta-thalassemia (ß-thalassemic) and sickle cell disease. The incidence of serious invasive bacterial infections was recorded. All patients received pneumococcal vaccine and all received oral prophylactic penicillin. RES ... | 2011 | 21866338 |
| guillian-barre syndrome. | guillian-barre syndrome (gbs) is the most common cause of acute flaccid paralysis. all age groups can be affected, more common in elderly. campylobacter jejuni, a major cause of bacterial gastroenteritis worldwide has become recognized as a most frequent antecedent pathogen for gbs. a prospective case-controlled study showed, positive c. jejuni serology was found in an unprecedented high frequency of 57% as compared to 8% in family controls and 3% in control patients with other neurological dise ... | 2011 | 22081202 |
| immunoreactive proteins of campylobacter concisus, an emergent intestinal pathogen. | campylobacter concisus is an emerging pathogen of the human gastrointestinal tract. recently, a significantly higher prevalence of c. concisus dna and higher levels of antibodies specific to c. concisus was detected in children with crohn's disease when compared with controls. the aim of this study was to identify c. concisus immunoreactive antigens. proteins from c. concisus were separated using two-dimensional gel electrophoresis, and sera from 10 c. concisus-positive children with crohn's dis ... | 2011 | 22092566 |
| campylobacter spp. as a foodborne pathogen: a review. | campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide. symptoms can range from mild to serious infections of the children and the elderly and permanent neurological symptoms. the organism is a cytochrome oxidase positive, microaerophilic, curved gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry. intestinal colonization results in healthy ... | 2011 | 21991264 |
| Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli Isolates from Domestic and Travel-Associated Human Cases. | Multilocus sequence typing (MLST) extended with flaB typing of 425 Campylobacter jejuni isolates and 42 Campylobacter coli isolates revealed quite a low overlap between human isolates from travel-associated and domestic cases in Switzerland. Men were more frequently affected by Campylobacter than women, but strains from women and, overall, from travel-associated cases showed mutations conferring quinolone resistance more frequently than strains from men and domestic cases, respectively. | 2012 | 22020515 |
| Campylobacteriosis rates show age-related static bimodal and seasonality trends. | Campylobacteriosis is highly characterised by a strongly seasonal rate of incidence. Age is also known to be a risk factor for sporadic campylobacteriosis, but little has been done to quantify age-related rates of campylobacteriosis. This study investigates age-related incidence across countries and up to 12 years of data, as well as differences in seasonality within age groups. | 2011 | 21946876 |
| prevalence and antimicrobial susceptibility of campylobacter in broiler flocks in japan. | campylobacter was isolated from 67 (47.2%) of 142 broiler flocks between september 2009 and february 2010. the prevalence of campylobacter in broiler flocks was significantly lower during january and february than it was from september to december. campylobacter colonization was more common in flocks that were not provided with a disinfected water supply, which was consistent with the findings of a previous study. the prevalence of antimicrobial drug-resistant campylobacter spp. was investigated ... | 2011 | 22103704 |
| comparison of campylobacter contamination levels on chicken carcasses between modern and traditional types of slaughtering facilities in malaysia. | a total of 360 samples including fresh fecal droppings, neck skins, and swab samples was collected from 24 broiler flocks and processed by 12 modern processing plants in 6 states in malaysia. ninety samples from 10 traditional wet markets located in the same states as modern processing plants were also collected. microbiological isolation for campylobacter was performed following iso 10272-1:2006 (e). the overall rate of contamination for campylobacter in modern processing plants and in traditio ... | 2011 | 21891977 |
| comparative in silico analysis of chemotaxis system of campylobacter fetus. | chemoreceptor and chemotaxis signal transduction cascade genes of c. fetus subsp. fetus 82-40 show high level of similarity to that in c. jejuni and appears to include sixteen diverse transducer-like protein (tlp) genes that appear similar to nine of the twelve tlp genes in the c. jejuni nctc 11168 with a percent identity ranging from 15 to 50%. sixteen putative c. fetus 82-40 tlp genes belong to three classes: a, b, and c, as well as an aerotaxis gene, based on their predicted structure. c. fet ... | 2011 | 21983836 |
| the cooperative action of bacterial fibronectin-binding proteins and secreted proteins promote maximal campylobacter jejuni invasion of host cells by stimulating membrane ruffling. | this study was performed to elucidate the host cell scaffolding and signalling molecules that campylobacter jejuni utilizes to invade epithelial cells. we hypothesized that the c. jejuni fibronectin-binding proteins and secreted proteins are required for cell signalling and maximal invasion of host cells. c. jejuni binding to host cells via the cadf and flpa fibronectin-binding proteins activated the epidermal growth factor (egf) pathway, as evidenced by inhibitor studies and immunoprecipitation ... | 2011 | 21999233 |
| genome sequences of two stress-tolerant campylobacter jejuni poultry strains, 305 and dfvf1099. | campylobacter jejuni is a food-borne pathogen with a high prevalence in poultry meat, which in fresh unfrozen condition is the major source of campylobacteriosis. c. jejuni strains dfvf1099 and 305 are considered tolerant to several environmental stresses (t. birk et al., j. food prot. 73:258-265, 2010; s. l. on et al., int. j. med. microbiol. 296:353-363, 2006). here, we report the genome sequences of c. jejuni 305 and dfvf1099, a turkey and a chicken isolate, respectively. | 2011 | 21914871 |
| Antimicrobial use: a risk factor or a protective factor for acquiring campylobacteriosis? | It is well acknowledged that the use of antimicrobial drugs in food animals leads to antimicrobial drug resistance in foodborne bacteria such as Campylobacter; however, the role of human antimicrobial usage is much less investigated. The aim of this study was to quantify the odds of campylobacteriosis conferred by human consumption of fluoroquinolones and macrolides. | 2011 | 21890769 |
| development of flexible antimicrobial packaging materials against campylobacter jejuni by incorporation of gallic acid into zein-based films. | in this study, antimicrobial films were developed against campylobacter jejuni by incorporation of gallic acid (ga) into zein-based films. the zein and zein-wax composite films containing ga between 2.5 and 10 mg/cm(2) were effective on different c. jejuni strains in a concentration-dependent manner. zein and zein-wax composite films showed different release profiles in distilled water but quite similar release profiles at solid agar medium. depending on incorporated ga concentration, 60-80% of ... | 2011 | 21905708 |
| poultry as a host for the zoonotic pathogen campylobacter jejuni. | abstract campylobacteriosis is the most reported foodborne gastroenteritic disease and poses a serious health burden in industrialized countries. disease in humans is mainly caused by the zoonotic pathogen campylobacter jejuni. due to its wide-spread occurrence in the environment, the epidemiology of campylobacter remains poorly understood. it is generally accepted, however, that chickens are a natural host for campylobacter jejuni, and for campylobacter spp. in general, and that colonized bro ... | 2011 | 22133236 |
| A Nationwide Study of Campylobacter jejuni and Campylobacter coli bacteremia in Finland over a 10-year period, 1998-2007, with special reference to clinical characteristics and antimicrobial susceptibility. | Campylobacter bacteremia is an uncommon condition, usually diagnosed in elderly and immunocompromised patients. | 2011 | 21921217 |
| [bacteremia and endarteritis cases secondary to campylobacter spp. in a metropolitan hospital: our experience along a quarter of a century]. | six cases of bacteremia and one of endarteritis were identified between 1986 and 2010 in a general hospital in chile. five of these cases occurred during the second half of this timeframe, campylobacter fetus predominated (5 out of 7) and the majority of the infections presented during warmer months. the mean age was 32.4 years (range 19 to 63) all had comorbidities, and main clinical manifestations included fever with diarrhea. four patients developed hypotension and two septic shock. the latte ... | 2011 | 21879145 |
| travel-associated salmonella and campylobacter gastroenteritis in england: estimation of under-ascertainment through national laboratory surveillance. | increased international travel raises the importance of accurate surveillance of travel-associated gastroenteric pathogens to improve treatment and the investigation of cross-border outbreaks. this study found that 45% of salmonella and 17% of campylobacter infections in england were travel-associated, but only 29 and 3% of travel histories were accurately identified by national laboratory surveillance. more structured data collection forms and staff training may be needed to address this. | 2011 | 22017719 |
| [Infectious diseases in Poland in 2009]. | With introduction of the new law on infectious diseases has decreased the number of infectious diseases subject to mandatory reporting. At the same time ambiguities in the text of the Act and no regulation defining the scope and manner of filing could affect the reduction in the number of applications especially in cases of gastro-intestinal infections caused by viruses. In 2009, a new variant of influenza A(H1N1)v has occurred in Mexico. Infection with this virus spread over all the continents ... | 2011 | 21913454 |
| An ancient molecule in a recalcitrant pathogen: the contributions of poly-P to the pathogenesis and stress responses of Campylobacter jejuni. | | 2011 | 22004028 |
| CASA chromogenic medium for enteric Campylobacter species. | We prospectively assessed stool samples from 370 patients for Campylobacter species by comparing three selective agar media incubated at two temperatures: 42°C and 37°C. Twenty patients (5.4%) were found positive. The chromogenic medium CASA (AES Chemunex, France) proved highly efficient for C. jejuni and C. coli recovery, while lessening the workload in the lab. | 2011 | 21849693 |
| Historical impact to drive research in peptic ulcer disease. | The story of gastric acid secretion began with early ideas on gastric secretion (Spallanzani and de Réaumur, 17th century) and with first descriptions of food digestion (Dupuytren and Bichat, Beaumont, early 18th century), followed by proof that gastric juice contained acid (Prout, early 18th century). The research continued with first descriptions of gastric glands as the source of gastric acid and its changes upon digestive stimulus (Purkinje and Golgi, mid and late 19th century). The theory o ... | 2011 | 22095008 |
| Comparison of molecular typing methods useful for detecting clusters of Campylobacter jejuni and C. coli isolates through routine surveillance. | Campylobacter spp. may be responsible for unreported outbreaks of foodborne disease. Detection of these outbreaks is made more difficult by the fact that the appropriate methods for detecting clusters of Campylobacter have not been well defined. We have compared the characteristics of five molecular typing methods on Campylobacter jejuni and C. coli isolates obtained from human and non-human sources during sentinel site surveillance over a three year period. Comparative genomic fingerprinting (C ... | 2011 | 22162562 |
| crystal structure of the multicopper oxidase from the pathogenic bacterium campylobacter jejuni cgug11284: characterization of a metallo-oxidase. | multicopper oxidases are a multi-domain family of enzymes that are able to couple oxidation of substrates with reduction of dioxygen to water. these enzymes are capable of oxidizing a vast range of substrates, varying from aromatic to inorganic compounds such as metals. this metallo-oxidase activity observed in several members of this family has been linked to mechanisms of homeostasis in different organisms. recently, a periplasmic multicopper oxidase, encoded by campylobacter jejuni, has been ... | 2011 | 22127520 |
| the prevalence of campylobacter in childhood diarrhoea in enugu state of nigeria. | this study was aimed at finding the prevalence rate of campylobacter in childhood diarrhea in enugu. the study covered a period of five years. all the specimens were set to come from children attending clinics in the university of nigeria teaching hospital, enugu, nigeria. five hundred and fourteen children (257 males and 257 females) within the age range of one and sixty months with diarrhea were investigated. all the stool specimens were processed for campylobacter by microscopy and culture us ... | 2009 | 22010500 |
| microbiological quality of australian sheep meat in 2004. | the third national baseline microbiological survey of australian sheep carcases and frozen boneless sheep meat was conducted in 2004. carcases (n=1117) sampled at 20 slaughter establishments were found to have a mean log total viable count (tvc, 25°c) of 2.28 cfu/cm(2) and escherichia coli was isolated from 43.0% carcases with a mean log 0.03cfu/cm(2) on positive samples. in samples from 10 boning (fabrication) plants (n=560) the mean log tvc for frozen boneless sheep meat was 1.85cfu/g and the ... | 2006 | 22062834 |
| N-linked glycosylation in Archaea: two paths to the same glycan. | N-linked protein glycosylation occurs in all three branches of life, eukaryotes, bacteria and archaea. The simplest system is that of the bacterium, Campylobacter jejuni, in which a heptasaccharide glycan is added to multiple proteins from a single lipid carrier molecule. In the eukaryotic system a conserved tetradecasaccharide modification is first added to target proteins, but is then modified by trimming and addition of other glycans from additional carrier molecules resulting in a diverse ar ... | 2011 | 21848800 |
| Source attribution, prevalence and enumeration of Campylobacter spp. from retail liver. | Campylobacter prevalence from retail liver (chicken, cattle, pig and sheep) was found to be 81%, 69%, 79% and 78% respectively. Molecular source attribution demonstrated that strains from chicken liver were most similar to those found commonly in humans. This provides further evidence of liver being a probable source of human infection. | 2011 | 22133565 |
| the th1/th2 paradigm in guillain-barré syndrome. | | 2011 | 21911705 |
| Keep the focus on contaminated poultry to further curtail New Zealand's campylobacteriosis epidemic. | | 2011 | 21946979 |
| campylobacter jejuni dna-binding protein from starved cells in guillain-barré syndrome patients. | campylobacter jejuni enteritis is frequently associated with an axonal form of guillain-barré syndrome (gbs) and c. jejuni dna-binding protein from starved cells (c-dps) induces paranodal myelin detachment and axonal degeneration through binding with sulfatide in vivo. here we investigated the invasion of c-dps into hosts with c. jejuni-related gbs. our analyses of patient sera found that both c-dps and anti-c-dps antibodies were most commonly detected in sera from c. jejuni-related gbs patients ... | 2011 | 21996079 |
| A Molecular Enrichment Strategy Based on cpn60 for Detection of Epsilon-Proteobacteria in the Dog Fecal Microbiome. | Members of the rare microbiome can be important components of complex microbial communities. For example, pet dog ownership is a known risk factor for human campylobacteriosis, and Campylobacter is commonly detected in dog feces by targeted assays. However, these organisms have not been detected by metagenomic methods. The goal of this study was to characterize fecal microbiota from healthy and diarrheic pet dogs using two different levels of molecular detection. PCR amplification and pyrosequen ... | 2011 | 21881944 |
| high prevalence of tritrichomonas foetus infection in asturiana de la montaña beef cattle kept in extensive conditions in northern spain. | bovine trichomonosis (bt) and bovine genital campylobacteriosis (bgc) are sexually transmitted diseases that can be important infectious causes of reproductive failure in extensively managed beef cattle where natural mating is a common practice. however, their prevalence in europe was thought to be insignificant or very low. the purpose of this study was to investigate the prevalence and risk factors associated with bt and bcg in a representative beef cattle breed, asturiana de la montaña (am), ... | 2011 | 22178360 |
| Reverse transcriptase real-time PCR for detection and quantification of viable Campylobacter jejuni directly from poultry faecal samples. | Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of viable Campylobacter jejuni directly from chicken faecal samples. The results of this method and a DNA-based quantitative real-time PCR (qPCR) method were compared with those of a ... | 2011 | 22064380 |
| Quality assessment of roof-harvested rainwater in the West Bank, Palestinian Authority. | Rain harvesting is becoming more common in the Palestinian Territories as a result of drinking water scarcity. Although it might pose serious human health risk, this water is being consumed without treatment in many areas of the West Bank. The present study evaluates the physicochemical and microbial quality of harvested rainwater that is used as potable water in the West Bank. Samples from roof-harvested rainwater storage tanks (n = 42) were collected in summer (SS) 2006/winter (WS) 2007. Physi ... | 2011 | 21976199 |
| challenges to meat safety in the 21st century. | the safety of meat has been at the forefront of societal concerns in recent years, and indications exist that challenges to meat safety will continue in the future. major meat safety issues and related challenges include the need to control traditional as well as "new," "emerging," or "evolving" pathogenic microorganisms, which may be of increased virulence and low infectious doses, or of resistance to antibiotics or food related stresses. other microbial pathogen related concerns include cross- ... | 2008 | 22062090 |
| campylobacter jejuni infection in guillain-barré syndrome: a prospective case control study in a tertiary care hospital. | guillain-barré syndrome (gbs), is a common post-infectious polyradiculoneuropathy worldwide. the commonest implicated causative organism the world over is campylobacter jejuni (c. jejuni). this study was carried out to determine the relationship between c. jejuni infection and gbs in an indian setting. | 2011 | 22019657 |
| subgingival microbial consortia and the clinical features of periodontitis in adolescents. | this study aimed to investigate the association between microbial consortia and the clinical features of periodontitis using a multilevel modeling approach. a total of 958 sites in 87 adolescents with periodontitis (cases) and 73 controls were microbiologically sampled and clinically examined. associations between each of the clinical parameters clinical attachment, probing depth, supragingival plaque, calculus, bleeding on probing, and each of 18 bacterial species; and between the same clinical ... | 2011 | 22112031 |
| systematic review: the use of proton pump inhibitors and increased susceptibility to enteric infection. | background: the use of proton pump inhibitors (ppis) is increasing worldwide. suppression of gastric acid alters the susceptibility to enteric bacterial pathogens. aim this systematic review was undertaken to examine the relationship between ppi use and susceptibility to enteric infections by a specific pathogen based on published literature and to discuss the potential mechanisms of ppi enhanced pathogenesis of enteric infections. methods pubmed, ovid medline databases were searched. search t ... | 2011 | 21999643 |
| investigation of six selected bacterial species in endo-periodontal lesions. | didilescu ac, rusu d, anghel a, nica l, iliescu a, greabu m, bancescu g, stratul si. investigation of six selected bacterial species in endo-periodontal lesions. international endodontic journal, 45, 282-293, 2012. abstract: aim to investigate and determine possible associations of six tested bacteria belonging to 'orange' and 'green' complexes, in endo-periodontal lesions: parvimonas micra, fusobacterium nucleatum, campylobacter rectus, eubacterium nodatum, eikenella corrodens and capnocytopha ... | 2011 | 22077868 |
| comparative analyses of campylobacter concisusstrains reveal the genome of the reference strain baa-1457 is not representative of the species. | abstract: | 2011 | 21992484 |
| complete genome sequence of type strain campylobacter fetus subsp. venerealis nctc 10354t. | campylobacter fetus subsp. venerealis is the etiologic agent of bovine genital campylobacteriosis, a sexually transmitted disease of cattle that is of worldwide importance. the complete sequencing and annotation of the genome of the type strain c. fetus subsp. venerealis nctc 10354(t) are reported. | 2011 | 21952544 |
| [Preparation and characterization of monoclonal antibodies specific for CjaA protein of Campylobacter jejuni]. | Expression, purification of Campylobacter jejuni CjaA protein and development of monoclonal antibodies (mAbs) against this protein. | 2011 | 22078449 |
| approach to the patient with infectious colitis. | to provide current recommendations for evaluation and treatment of patients with infectious colitis. infectious colitis is diagnosed in someone with diarrhea and one or more of the following: fever and/or dysentery, stools containing inflammatory markers such as leukocytes, lactoferrin, or calprotectin, or positive stool culture for an invasive or inflammatory bacterial enteropathogen including shigella, salmonella, campylobacter, shiga toxin-producing escherichia coli (stec) or clostridium diff ... | 2012 | 22080825 |
| diversity and impact of prokaryotic toxins on aquatic environments: a review. | microorganisms are ubiquitous in all habitats and are recognized by their metabolic versatility and ability to produce many bioactive compounds, including toxins. some of the most common toxins present in water are produced by several cyanobacterial species. as a result, their blooms create major threats to animal and human health, tourism, recreation and aquaculture. quite a few cyanobacterial toxins have been described, including hepatotoxins, neurotoxins, cytotoxins and dermatotoxins. these t ... | 2010 | 22069558 |
| Ribosomal mutations as the main cause of macrolide resistance in Campylobacter jejuni and Campylobacter coli. | The aim of this study was to examine macrolide resistance mutations in Campylobacter species. In 76 strains studied, point mutation A to G at position 2059 of the 23S rRNA gene was detected in 30 of the 33 erythromycin-resistant strains. An amino acid insertion in the ribosomal protein L22 was found in one resistant strain without a 23S rRNA mutation. The A2059G mutation is the main cause of macrolide resistance in Campylobacter species. | 2011 | 21911571 |
| Molecular Techniques in Ecohealth Research Toolkit: Facilitating Estimation of Aggregate Gastroenteritis Burden in an Irrigated Periurban Landscape. | Assessment of microbial hazards associated with certain environmental matrices, livelihood strategies, and food handling practices are constrained by time-consuming conventional microbiological techniques that lead to health risk assessments of narrow geographic or time scope, often targeting very few pathogens. Health risk assessment based on one or few indicator organisms underestimates true disease burden due a number of coexisting causative pathogens. Here, we employed molecular techniques i ... | 2011 | 22146856 |
| roles of rpon in the resistance of campylobacter jejuni under various stress conditions. | campylobacter jejuni is a leading foodborne pathogen worldwide. despite the fastidious nature of c. jejuni growth, increasing numbers of human campylobacteriosis suggest that c. jejuni may possess unique mechanisms to survive under various stress conditions. c. jejuni possesses only three sigma factors (flia, rpod, and rpon) and lacks stress-defense sigma factors. since flia and rpod are dedicated to flagella synthesis and housekeeping, respectively, in this study, we investigated the role of rp ... | 2011 | 21939540 |
| motility and chemotaxis in campylobacter and helicobacter . | flagellar motility of campylobacter jejuni and helicobacter pylori influences host colonization by promoting migration through viscous milieus such as gastrointestinal mucus. this review explores mechanisms c. jejuni and h. pylori employ to control flagellar biosynthesis and chemotactic responses. these microbes tightly control the activities of σ(54) and σ(28) to mediate ordered flagellar gene expression. in addition to phase-variable and posttranslational mechanisms, flagellar biosynthesis is ... | 2011 | 21939377 |
| epidemiologic characteristics of human campylobacteriosis in the county primorsko-goranska (croatia), 2003-2007. | the aim of the study was to investigate campylobacteriosis incidence in the county primorsko-goranska (croatia) between 2003 and 2007 and to find out possible connection with environmental factors (the average monthly temperature and total monthly precipitation). the data (number of stool samples examined, age and sex distribution of patients, monthly distribution of isolates and distribution of isolates according to the species) from the laboratory for diagnostics of enteric infections of the t ... | 2011 | 22053566 |
| Enteropathogenic bacteria in dogs and cats: diagnosis, epidemiology, treatment, and control. | This report offers a consensus opinion on the diagnosis, epidemiology, treatment, and control of the primary enteropathogenic bacteria in dogs and cats, with an emphasis on Clostridium difficile, Clostridium perfringens, Campylobacter spp., Salmonella spp., and Escherichia coli associated with granulomatous colitis in Boxers. Veterinarians are challenged when attempting to diagnose animals with suspected bacterial-associated diarrhea because well-scrutinized practice guidelines that provide obje ... | 2011 | 22092607 |
| Could Helicobacter pylori play a role in the aetiopathogenesis of tympanosclerosis? | The aetiology of tympanosclerosis is not yet clear. This prospective, controlled, clinical study investigated the relationship between Helicobacter pylori and tympanosclerosis aetiology. | 2011 | 21888743 |
| [Etiological aspects of acute gastroenteritis--a ten-year review (1.01. 2001-31.12.2010)]. | Acute gastroentritis is one of the most common diseases in humans, and continues to be a significant cause of morbidity worldwide. | 2011 | 22046776 |
| development of a sensitive rrna-targeted reverse transcription-quantitative polymerase chain reaction for detection of vibrio cholerae/mimicus, v. parahaemolyticus/alginolyticus and campylobacter jejuni/coli. | a sensitive rrna-targeted reverse transcription-quantitative polymerase chain reaction (rt-qpcr) method was developed for detection of vibrio cholerae/mimicus, v. parahaemolyticus/alginolyticus and campylobacter jejuni/coli by using specific primers. counts of the enteric pathogens spiked in human stools were quantified at the lower detection limit of 10(3) cells /g of stools by rt-qpcr, in marked contrast with conventional quantitative polymerase chain reaction (qpcr) at the detection limit of ... | 2011 | 22146006 |
| Microbiota in the oral subgingival biofilm is associated with obesity in adolescence. | To test the hypothesis whether microbiota in oral biofilm is linked with obesity in adolescents we designed this cross-sectional study. Obese adolescents (n = 29) with a mean age of 14.7 years and normal weight subjects (n = 58) matched by age and gender were examined with respect to visible plaque index (VPI%) and gingival inflammation (bleeding on probing (BOP%)). Stimulated saliva was collected. They answered a questionnaire concerning medical history, medication, oral hygiene habits, smoking ... | 2012 | 21996660 |
| from farm to fork follow-up of thermotolerant campylobacters throughout the broiler production chain and in human cases in a hungarian county during a ten-months period. | a study tracking thermotolerant campylobacters from the setting of the broilers throughout the whole rearing period, slaughter and sale of chicken products in five consecutive broiler rotations of the same henhouse as well as in two different other farms was conducted in a well-defined geographic area (hajdú-bihar county, hungary) between march 2006 and feb 2007. all notified cases of human campylobacteriosis in this area during the study period were also included. one hundred and one, 44, 23 an ... | 2011 | 21864930 |
| long-term trends in the epidemiology and resistance of childhood bacterial enteropathogens in crete. | in this study, we investigated the long-term trends in the epidemiology and susceptibility of bacterial enteropathogens among children in a well-defined area of adequate health standards. the study included all children younger than 14 years of age treated for enteritis at heraklion university general hospital on the island of crete during the 18-year period from january 1993 to december 2010. stool specimens were tested for salmonella, shigella, campylobacter, enteropathogenic escherichia coli ... | 2011 | 22207394 |
| The clinical importance of emerging Campylobacter species. | A growing number of Campylobacter species other than C. jejuni and C. coli have been recognized as emerging human and animal pathogens. Although C. jejuni continues to be the leading cause of bacterial gastroenteritis in humans worldwide, advances in molecular biology and development of innovative culture methodologies have led to the detection and isolation of a range of under-recognized and nutritionally fastidious Campylobacter spp., including C. concisus, C. upsaliensis and C. ureolyticus. T ... | 2011 | 22025030 |
| post-harvest interventions to reduce/eliminate pathogens in beef. | in 1999 the foodborne pathogens salmonella, listeria, campylobacter, and escherichia coli (both o157 and non-o157) were estimated to cause more than 6 million illnesses and approximately 9000 deaths each year. however, the most recent centers for disease control and prevention report on the sources and incidence of foodborne disease, released in 2004, has shown a dramatic decrease in e. coli o157:h7 infections. since raw beef products are the most frequently foodborne sources of these pathogens, ... | 2005 | 22064054 |
| Improvement of Modified Charcoal-Cefoperazone-Deoxycholate Agar by Supplementation with a High Concentration of Polymyxin B for the Detection of Campylobacter jejuni and C. coli in Chicken Carcass Rinse. | Modified charcoal-cefoperazone-deoxycholate agar (mCCDA) was improved by supplementation with a high concentration of polymyxin B. The ability of the supplemented medium to isolate Campylobacter jejuni and C. coli from chicken carcass rinse was compared to that of Campy-Cefex agar and mCCDA. Modification of mCCDA with increased polymyxin B yielded significantly (p < 0.05) better isolation rate and selectivity than those achieved on using Campy-Cefex agar and mCCDA. | 2011 | 22210208 |
| Identification of Immunogenic and Virulence associated Campylobacter jejuni Proteins. | With the aim of identifying proteins important for host interaction and virulence, we have screened an expression library of NCTC 11168 C. jejuni genes for highly immunogenic proteins. A commercial C. jejuni ORF library consisting of more than 1600 genes was transformed into the E. coli expression strain BL21 (DE3) resulting in 2304 clones. This library was subsequently screened for immunogenic proteins using antibodies raised in rabbit against a clinical isolate of C. jejuni; this resulted in 5 ... | 2011 | 22155767 |
| Granulated lysozyme as an alternative to antibiotics improves growth performance and small intestinal morphology of 10-day-old pigs. | Lysozyme is a 1,4-ß-N-acetylmuramidase that has antimicrobial properties. The objective of this experiment was to determine the effect of a purified granulated lysozyme, compared to antibiotics, on growth performance, small intestinal morphology, and Campylobacter shedding in 10-d-old pigs. Forty-eight pigs (n = 16), with an initial weight of 4.0 ± 0.1 kg (P > 0.40) were weaned at 10 d of age, blocked by litter and gender, and assigned to pens (8 pigs/pen). Each block was randomly assigned to co ... | 2011 | 22064735 |
| genotypes and antibiotic resistance of campylobacter coli in fattening pigs. | campylobacter coli is a food-borne zoonotic pathogen causing human gastroenteritis worldwide. the organism is a commensal in the intestine of many food production animals including fattening pigs. the role of the pig as a potential reservoir for c. coli affecting human either directly or via poultry has hardly been investigated and genetic characterization of porcine strains is needed to address this question. for this aim multilocus sequence typing (mlst) and flab typing was applied to 256 c. c ... | 2011 | 21899961 |