| comparative analysis of protaper and waveone systems to reduce enterococcus faecalis from root canal system in primary molars--an in vitro study. | to assess, in vitro, the ability of the protaper(™) and waveone(™) systems to reduce enterococcus faecalis contamination in primary molars. | 2016 | 26950813 |
| in silico dissection of type vii secretion system components across bacteria: new directions towards functional characterization. | type vii secretion system (t7ss) is one of the factors involved in virulence of mycobacterium tuberculosis h37rv. numerous research efforts have been made in the last decade towards characterizing the components of this secretion system. an extensive genome-wide analysis through compilation of isolated information is required to obtain a global view of diverse characteristics and pathogenicity-related aspects of this machinery. the present study suggests that differences in structural components ... | 2016 | 26949095 |
| invasive enterococcal infections in poland: the current epidemiological situation. | the aim of this study was to investigate human invasive isolates of enterococci, obtained through prospective surveillance in poland. the consecutive enterococcal isolates were collected in 30 hospitals between may 2010 and june 2011, and studied by species identification, antimicrobial susceptibility testing and, for enterococcus faecium by detection of markers specific for the hospital meroclone, multilocus vntr analysis (mlva) and multilocus sequence typing (mlst). additionally, the genomic d ... | 2016 | 26946510 |
| antibacterial and cytotoxic triterpenoids from the roots of combretum racemosum. | a new pentacyclic triterpenoid glucoside, together with fourteen known compounds, was isolated from the roots of combretum racemosum. combretaceae). the structure of the new compound was established as 28-o-β-d-glucopyranosyl-2α,3β,21β,23-tetrahydroxyolean-18-en-28-oate (1) on the basis of detailed spectroscopic data including ms, 1d, and 2d nmr. the inhibitory activity of compounds 1-15 against promyelocytic leukemia hl-60 and human erythromyeloblastoid leukemia k562 cell lines was evaluated. c ... | 2016 | 26946378 |
| time is of the essence: the impact of delayed antibiotic therapy on patient outcomes in hospital-onset enterococcal bloodstream infections. | with increasing prevalence of vancomycin-resistant enterococci (vre), appropriate antibiotic therapy for enterococcal bloodstream infections (ebsi) can be delayed. data regarding the impact of delayed therapy on ebsi outcomes are conflicting, and the time delay most strongly associated with poor outcomes has not been defined. | 2016 | 26945013 |
| occurrence of vancomycin-resistant and -susceptible enterococcus spp. in reclaimed water used for spray irrigation. | reclaiming municipal wastewater for agricultural, environmental, and industrial purposes is increasing in the united states to combat dwindling freshwater supplies. however, there is a lack of data regarding the microbial quality of reclaimed water. in particular, no previous studies have evaluated the occurrence of vancomycin-resistant enterococci (vre) in reclaimed water used at spray irrigation sites in the united states. to address this knowledge gap, we investigated the occurrence, concentr ... | 2016 | 26942838 |
| antimicrobial activity of herbal medicines (tulsi extract, neem extract) and chlorhexidine against enterococcus faecalis in endodontics: an in vitro study. | successful endodontic treatment depends on effective disinfection and complete sealing of root canal. various medicaments are advised for disinfecting root canal, such as herbal and non-herbal medicaments. this study was done to assess the antimicrobial activity of herbal medicines (neem extract, tulsi extract) and chlorhexidine against enterococcus faecalis in endodontics. | 2015 | 26942123 |
| killing of vre enterococcus faecalis by commensal strains: evidence for evolution and accumulation of mobile elements in the absence of competition. | enterococci are members of the gastrointestinal tract of humans and most animals that, over the past 3 decades, have emerged as leading causes of multidrug resistant hospital acquired infection (hai). in addition to their general hardiness, many traits have entered enterococcal lineages through horizontal gene transfer, which has led to the evolution of pathogenic hospital-associated lineages uniquely adapted for survival and proliferation in the antibiotic perturbed ecology of the gastrointesti ... | 2016 | 26939857 |
| comparative antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), mtad, and 2.5% sodium hypochlorite against enterococcus faecalis: an in vitro study. | the objective of this study was to evaluate the antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), biopure mtad, and 2.5% sodium hypochlorite against enterococcus faecalis. | 2017 | 26937369 |
| in vitro antimicrobial evaluation of toothpastes with natural compounds. | this in vitro study evaluated the antimicrobial effects of commercial toothpastes containing natural compounds. | 2017 | 26929699 |
| ampicillin in combination with ceftaroline, cefepime, or ceftriaxone demonstrates equivalent activities in a high-inoculum enterococcus faecalis infection model. | ampicillin-ceftriaxone combination therapy has become a predominant treatment for serious enterococcus faecalis infections, such as endocarditis. unfortunately, ceftriaxone use is associated with future vancomycin-resistant enterococcus colonization. we evaluated e. faecalis in an in vitro pharmacodynamic model against simulated human concentration-time profiles of ampicillin plus ceftaroline, cefepime, ceftriaxone, or gentamicin. ampicillin-cefepime and ampicillin-ceftaroline demonstrated activ ... | 2016 | 26926624 |
| lipoteichoic acid of enterococcus faecalis inhibits the differentiation of macrophages into osteoclasts. | enterococcus faecalis is associated with persistent endodontic infection and refractory apical periodontitis. recently, we have shown that heat-killed e. faecalis attenuates osteoclast differentiation. because lipoteichoic acid (lta) is a major virulence factor of gram-positive bacteria, we investigated the effect of lta from e. faecalis (eflta) on osteoclast differentiation. | 2016 | 26920932 |
| envelope structures of gram-positive bacteria. | gram-positive organisms, including the pathogens staphylococcus aureus, streptococcus pneumoniae, and enterococcus faecalis, have dynamic cell envelopes that mediate interactions with the environment and serve as the first line of defense against toxic molecules. major components of the cell envelope include peptidoglycan (pg), which is a well-established target for antibiotics, teichoic acids (tas), capsular polysaccharides (cps), surface proteins, and phospholipids. these components can underg ... | 2016 | 26919863 |
| the impact of host metapopulation structure on the population genetics of colonizing bacteria. | many key bacterial pathogens are frequently carried asymptomatically, and the emergence and spread of these opportunistic pathogens can be driven, or mitigated, via demographic changes within the host population. these inter-host transmission dynamics combine with basic evolutionary parameters such as rates of mutation and recombination, population size and selection, to shape the genetic diversity within bacterial populations. whilst many studies have focused on how molecular processes underpin ... | 2016 | 26916623 |
| synthesis and biological activities of lanthanide (iii) nitrate complexes with n-(2-hydroxynaphthalen-1-yl) methylene) nicotinohydrazide schiff base. | the field of coordination chemistry has registered a phenomenal growth during last few decades. it is well known that precious metals have been used for medicinal purposes for at least 3500 years. at that time, precious metals were believed to benefit health because of their rarity, but research has now well established the link between medicinal properties of inorganic drugs and specific biological properties. | 2016 | 26916500 |
| in vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers. | the aim of this study was to evaluate whether the modification in the silver component is capable of providing guttaflow 2 with antibacterial activity against enterococcus faecalis compared with epoxy resin-based (ah plus) and zinc oxide and eugenol-based (endofill) sealers. the antibacterial activity was evaluated using a reference strain of e. faecalis (atcc 29212). freshly mixed sealers were subjected to the agar diffusion test (adt), while the direct contact test (dct) was performed after ma ... | 2016 | 26910015 |
| screening of the enterocin-encoding genes and antimicrobial activity in enterococcus species. | in the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes enta, entp, entb, entl50a, and entl50b. the enterocin genes were present at different frequencies, with enta occurring the most frequently, followed by entp and entb; entl50a and l50b were not detected. the occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. the 80 isolates that harbor at least one ... | 2016 | 26907753 |
| prevalence and risk factors of early fecal carriage of enterococcus faecalis and staphylococcus spp and their antimicrobial resistant patterns among healthy neonates born in a hospital setting in central saudi arabia. | to investigate the prevalence, antibiotic resistant profiles, and risk factors of early fecal carriage of enterococcus faecalis (e. faecalis) and staphylococci among 150 healthy saudi neonates born in a hospital setting in central saudi arabia. | 2016 | 26905350 |
| evolution of resistance to cationic biocides in streptococcus mutans and enterococcus faecalis. | the aim of this study was to investigate whether streptococcus mutans and enterococcus faecalis develop resistance to the cationic biocides chlorhexidine (chx), cetylpyridinium chloride (cpc), and 12-methacryloyloxydodecylpyridinium bromide (mdpb). | 2016 | 26904979 |
| genetic environment of the transferable oxazolidinone/phenicol resistance gene optra in enterococcus faecalis isolates of human and animal origin. | aim of this study was to analyse 17 non-related enterococcus faecalis isolates of human and animal origin for the genetic environment of the novel oxazolidinone/phenicol resistance gene optra. | 2016 | 26903276 |
| evaluation of polymorphisms in pbp4 gene and genetic diversity in penicillin-resistant, ampicillin-susceptible enterococcus faecalis from hospitals in different states in brazil. | the aim of the present study was to verify whether penicillin-resistant, ampicillin-susceptible enterococcus faecalis (prasef) occurred in brazil prior to the beginning of the 21st century, and to verify whether ampicillin susceptibility can predict susceptibility to other β-lactams in e. faecalis with this inconsistent phenotype. the presence of polymorphisms in the pbp4 gene and genetic diversity among the isolates were investigated. of 21 prasef analyzed, 5 (23.8%) and 4 (19.0%) were imipenem ... | 2016 | 26903013 |
| clinical characteristics and antimicrobial patterns in complicated intra-abdominal infections: a 6-year epidemiological study in southern china. | complicated intra-abdominal infection (ciais) are a common and important cause of morbidity worldwide. in this study, the clinical features, microbiological profiles, antimicrobial patterns and treatments of 3233 ciai patients (mean age, 47.6 years; 54.7% male) with 3531 hospitalisations from 2008-2013 were retrospectively investigated. the most commonly isolated bacteria were escherichia coli (47.6%), klebsiella pneumoniae (16.9%), enterococcus faecalis (10.4%) and pseudomonas aeruginosa (8.8%) ... | 2016 | 26899413 |
| comparison of telavancin and vancomycin lock solutions in eradication of biofilm-producing staphylococci and enterococci from central venous catheters. | results of a study of the activity of antibiotic lock solutions of vancomycin and telavancin against biofilm-forming strains of staphylococcus epidermidis, enterococcus faecalis, and staphylococcus aureus are reported. | 2016 | 26896504 |
| [clinical observations of acute-onset endophthalmitis after clear corneal phacoemulsification]. | to evaluate the incidence of acute-onset endophthalmitis after clear corneal phacoemulsification and to report its clinical features, microbiology, and therapeutic efficacy. | 2015 | 26888274 |
| to comparatively evaluate the antimicrobial efficacy of chlorhexidine, nisin and linezolid as an intracanal medicament on enterococcus faecalis: an in vitro study. | the present study evaluated the antimicrobial efficacy of chlorhexidine, nisin and linezolid and a control group (normal saline ) against enterococcus faecalis (ef). | 2016 | 26888241 |
| the influence of urinary tract infections on treatment of fracture of proximal femur in patients with systemiv osteoporosis. | in the structure of intrahospital infections the infections of urinary tract make up from 20 to 40%. this problem represents special medical-social significance for trauma departments as patients with trauma are being in forced situation for along time. 380 patients older than 60 years (average age 73.6 ± 9.2 years) with a fracture of proximal femur against background of the systemic osteoporosis having in-patient treatment in the trauma department of the republic hospital no2 were being examine ... | 2015 | 26887137 |
| a new mechanism of post-transfer editing by aminoacyl-trna synthetases: catalysis of hydrolytic reaction by bacterial-type prolyl-trna synthetase. | aminoacyl trna synthetases are enzymes that specifically attach amino acids to cognate trnas for use in the ribosomal stage of translation. for many aminoacyl trna synthetases, the required level of amino acid specificity is achieved either by specific hydrolysis of misactivated aminoacyl-adenylate intermediate (pre-transfer editing) or by hydrolysis of the mischarged aminoacyl-trna (post-transfer editing). to investigate the mechanism of post-transfer editing of alanine by prolyl-trna synthetas ... | 2017 | 26886480 |
| antibacterial effect of hydroalcoholic extract of punica granatum linn. petal on common oral microorganisms. | objectives. this study aimed to assess the effect of hydroalcoholic extract of punica granatum linn. (p. granatum) petal on streptococcus sanguinis, streptococcus mutans, streptococcus salivarius, streptococcus sobrinus, and enterococcus faecalis. materials and methods. in this in vitro study, p. granatum extract was prepared using powdered petals and water-ethanol solvent. antibacterial effect of the extract, chlorhexidine (chx), and ampicillin was evaluated on brain heart infusion agar (bhia) ... | 2016 | 26884763 |
| functional dissection of the crors two-component system required for resistance to cell wall stressors in enterococcus faecalis. | bacteria use two-component signal transduction systems (tcss) to sense and respond to environmental changes via a conserved phosphorelay between a sensor histidine kinase and its cognate response regulator. the opportunistic pathogen enterococcus faecalis utilizes a tcs comprised of the histidine kinase cros and the response regulator cror to mediate resistance to cell wall stresses such as cephalosporin antibiotics, but the molecular details by which crors promotes cephalosporin resistance have ... | 2016 | 26883822 |
| bacteriospermia and its impact on basic semen parameters among infertile men. | introduction. semen analysis is considered as the surrogate marker for male fecundity while assessing infertile men. there are several reasons for altered semen quality and bacteriospermia could be one among them. thereby the aim of our work is to study the semen culture and its impact on semen parameters among infertile men. materials and methods. semen samples were collected from men attending infertility clinic. semen parameters were analysed based on who guidelines. also, samples were subjec ... | 2016 | 26880908 |
| electrochemical disinfection of simulated ballast water on pbo2/graphite felt electrode. | a novel pbo2/graphite felt electrode was constructed by electrochemical deposition of pbo2 on graphite felt and characterized by x-ray powder diffraction (xrd) and scanning electron microscopy (sem) analysis. the prepared electrode is a viable technology for inactivation of escherichia coli, enterococcus faecalis, and artemia salina as indicator organisms in simulated ballast water treatment, which meets the international maritime organization (imo) regulation d-2. the effects of contact time an ... | 2016 | 26880130 |
| isoflavone metabolism by a collection of lactic acid bacteria and bifidobacteria with biotechnological interest. | almost all soy isoflavones exist as glycosides, daidzin, genistin, and glycitin. we analyzed the capacity of 92 strains of lactic acid bacteria (lab) and bifidobacteria with biotechnological interest to process the glycosylated isoflavones daidzin, genistin, and glycitin in their more bioavailable aglycones and their metabolites as dihydrodaidzein (dhd), o-desmethylangolensin, and equol. representative strains of the four genera studied lactobacillus, enterococcus, lactococcus, and bifidobacteri ... | 2016 | 26878882 |
| cholesterol suppresses antimicrobial effect of statins. | isoprenoid biosynthesis is a key metabolic pathway to produce a wide variety of biomolecules such as cholesterol and carotenoids, which target cell membranes. on the other hand, it has been reported that statins known as inhibitors of isoprenoid biosynthesis and cholesterol lowering agents, may have a direct antimicrobial effect on the some bacteria. the exact action of statins in microbial metabolism is not clearly understood. it is possible that statins inhibit synthesis or utilization of some ... | 2015 | 26877857 |
| enterococcus faecium small colony variant endocarditis in an immunocompetent patient. | small colony variants (scv) are slow-growing subpopulations of bacteria usually associated with auxotrophism, causing persistent or recurrent infections. enterococcus faecalis scv have been seldom described, and only one case of enterococcus faecium scv has been reported, associated with sepsis in a leukaemia patient. here we report the first case described of bacteraemia and endocarditis by scv e. faecium in an immunocompetent patient. | 2016 | 26862434 |
| antibiofilm efficacy of honey and bee-derived defensin-1 on multi-species wound biofilm. | many clinically relevant biofilms are polymicrobial. examining the effect of antimicrobials in a multi-species biofilm consortium is of great clinical importance. the goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multi-species biofilm and to test the antibiofilm activity of honey defensin-1 in its recombinant form. modified lubbock chronic wound biofilm formed by four bacterial species (staphylococcus aureus, streptococcus ag ... | 2016 | 26861950 |
| field study on evaluation of the efficacy and usability of two disinfectants for drinking water treatment at small cattle breeders and dairy cattle farms. | the hygienic quality of drinking water for cattle originated from different sources together with the efficacy and usability of two types of disinfectants against waterborne pathogens were assessed for small cattle breeders and dairy cattle farms. a total of 120 drinking water samples were collected from water troughs representing three different water sources commonly used for cattle drinking (tap, underground and surface water; n = 65, 25, and 30, respectively). collected samples were cultured ... | 2016 | 26861741 |
| photoactivated disinfection (pad) of dental root canal system - an ex-vivo study. | to investigate the efficacy of photo activated disinfection (pad) in reducing colony-forming unit (cfu) counts of enterococcus faecalis (e. faecalis) in infected dental root canals. the study compared the efficacy of pad with conventional endodontic treatment (cet) and also a combination of cet along with pad. | 2016 | 26858548 |
| [in vitro activity of ampicillin-ceftriaxone against enterococcus faecalis isolates recovered from invasive infections]. | in vitro activity of the combination of ampicillin- ceftriaxone against 30 enterococcus faecalis isolates recovered from invasive infections in patients admitted to hospital de clínicas josé de san martin in the city of buenos aires was assessed. ampicillin- ceftriaxone synergies were determined by microdilution in müeller-hinton (mh) broth with and without subinhibitory concentrations of ceftriaxone. synergy was detected in 22/30 isolates. a decrease in both minimum inhibitory concentration (mi ... | 2016 | 26857425 |
| an analysis in vivo of intracanal bacterial load before and after chemo-mechanical preparation: a comparative analysis of two irrigants and two activation techniques. | the goals of this randomized double-blind trial were to assess the antimicrobial activity in vivo of sodium hypochlorite (naocl) vs. chlorhexidine gluconate (chx) used in combination either with endoactivator® or irri s® files in patients with apical periodontitis. | 2016 | 26855714 |
| the effects of silver nanoparticles on antimicrobial activity of proroot mineral trioxide aggregate (mta) and calcium enriched mixture (cem). | although, mineral trioxide aggregate (mta) and new experimental cement (cem) are good root filling cements, but had no or low antimicrobial activities. the aim of this study was to evaluate the effects of addition of silver nanoparticles (snp) to these two cements on antimicrobial effects against five most dental infection related microorganisms. | 2016 | 26855701 |
| did the iceman know better? screening of the medicinal properties of the birch polypore medicinal mushroom, piptoporus betulinus (higher basidiomycetes). | the birch polypore piptoporus betulinus was among two mushrooms that were found in the iceman's bag. recent studies indicated that p. betulinus was probably used as a religious and medicinal item. in order to examine the medicinal potential of p. betulinus, hot water (hw), partially purified (pp), and alkali extract (ha) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin i-converting enzyme (ace) inhibitory activity. all tested samples exhibited moderate cytotoxi ... | 2015 | 26854098 |
| incidence of virulence determinants in clinical enterococcus faecalis and enterococcus faecium isolates collected in bulgaria. | to evaluate the prevalence of some virulence genes among 510 clinical enterococcus spp. isolates and to assess the association of those genes with the species, infection site, and patient group (inpatients/outpatients). | 2016 | 26849965 |
| antimicrobial and cytotoxic activity of cuminum cyminum as an intracanal medicament compared to chlorhexidine gel. | the aims of this study were i) to define the chemical constituents of cuminum cyminum (cumin) essential oil, ii) to compare the antimicrobial activity of this oil to that of chlorhexidine (chx) and co-trimoxazole on planktonic and biofilm forms of bacteria isolated from the teeth with persistent endodontic infection and iii ) to compare the cytotoxicity of these medicaments on l929 fibroblasts. | 2016 | 26843877 |
| antibacterial activity of diode laser and sodium hypochlorite in enterococcus faecalis-contaminated root canals. | the aim of the present in vitro study was to evaluate the disinfection ability of 980-nm diode laser in comparison with sodium hypochlorite (naocl) as a common root canal irrigant in canals infected with enterococcus faecalis (e. faecalis). | 2016 | 26843870 |
| effectiveness of different disinfection protocols on the reduction of bacteria in enterococcus faecalis biofilm in teeth with large root canals. | this study compared the effectiveness of different disinfection protocols in reducing bacteria in an enterococcus faecalis biofilm in teeth with large root canals. | 2016 | 26830428 |
| cardiac surgery for infective endocarditis in patients with intravenous drug use. | intravenous drug users have a high risk of infective endocarditis and reduced survival. cardiac surgery may be recommended for these patients, but redo surgery is controversial. this study describes the characteristics and outcomes of intravenous drug users accepted for surgery during a 12-year period. | 2016 | 26826713 |
| γ-cyclodextrin capped silver nanoparticles for molecular recognition and enhancement of antibacterial activity of chloramphenicol. | computational studies were conducted to identify the favourable formation of the inclusion complex of chloramphenicol with cyclodextrins. the results of molecular docking and molecular dynamics predicted the strongest interaction of chloramphenicol with γ-cyclodextrin. further, the inclusion complex of chloramphenicol with γ-cyclodextrin was experimentally prepared and a phenomenon of inclusion was verified by using different characterization techniques such as thermogravimetric analysis, differ ... | 2016 | 26824520 |
| development and characterization of potent cyclic acyldepsipeptide analogues with increased antimicrobial activity. | the problem of antibiotic resistance has prompted the search for new antibiotics with novel mechanisms of action. analogues of the a54556 cyclic acyldepsipeptides (adeps) represent an attractive class of antimicrobial agents that act through dysregulation of caseinolytic protease (clpp). previous studies have shown that adeps are active against gram-positive bacteria (e.g., mrsa, vre, prsp (penicillin-resistant streptococcus pneumoniae)); however, there are currently few studies examining gram-n ... | 2016 | 26818454 |
| [the incidence and risk factors of male urinary tract infection following invasive urodynamic investigation]. | to assess the prevalence of male urinary tract infection (uti) after invasive urodynamic studies and to determinate the risk factors by evaluating the clinical characteristics and urodynamic parameters of patients. | 2015 | 26813139 |
| redefining healthy urine: a cross-sectional exploratory metagenomic study of people with and without bladder dysfunction. | we used the pathoscope platform to perform species level analyses of publicly available, 16s rrna pyrosequenced, asymptomatic urine data to determine relationships between microbiomes, and clinical and functional phenotypes. | 2016 | 26807926 |
| monitoring the effectiveness of photodynamic therapy with periodic renewal of the photosensitizer on intracanal enterococcus faecalis biofilms. | photodynamic therapy (pdt) can eliminate microorganisms in a root canal. however, the parameters for disinfection remain undefined. this study assessed the effectiveness of a pdt protocol against intracanal enterococcus faecalis biofilms. | 2016 | 26796030 |
| vinegar treatment prevents the development of murine experimental colitis via inhibition of inflammation and apoptosis. | this study investigated the preventive effects of vinegar and acetic acid (the active component of vinegar) on ulcerative colitis (uc) in mice. vinegar (5% v/v) or acetic acid (0.3% w/v) treatment significantly reduced the disease activity index and histopathological scores, attenuated body weight loss, and shortened the colon length in a murine experimental colitis model induced by dextran sulfate sodium (dss). further mechanistic analysis showed that vinegar inhibited inflammation through supp ... | 2016 | 26795553 |
| enterococcus faecalis from food, clinical specimens, and oral sites: prevalence of virulence factors in association with biofilm formation. | enterococci have gained significance as the cause of nosocomial infections; they occur as food contaminants and have also been linked to dental diseases. e. faecalis has a great potential to spread virulence as well as antibiotic resistance genes via horizontal gene transfer. the integration of food-borne enterococci into the oral biofilm in-vivo has been observed. therefore, we investigated the virulence determinants and antibiotic resistance of 97 e. faecalis isolates from the oral cavity, foo ... | 2015 | 26793174 |
| global regulation of gene expression by the mafr protein of enterococcus faecalis. | enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. however, as an opportunistic pathogen, it is able to colonize other host niches and cause life-threatening infections. its adaptation to new environments involves global changes in gene expression. the ef3013 gene (here named mafr) of e. faecalis strain v583 encodes a protein (mafr, 482 residues) that has sequence similarity to global response regulators of the mga/atxa family. the enterococcal og1rf genome also e ... | 2015 | 26793169 |
| partial purification and characterization of bacteriocin produced by enterococcus faecalis du10 and its probiotic attributes. | a novel bacteriocin produced by avian duck isolated lactic acid bacterium enterococcus faecalis du10 was isolated. this bacteriocin showed a broad spectrum of antibacterial activity against important food-borne pathogens and was purified by size exclusion chromatography followed by reverse-phase high-performance liquid chromatography in a c-18 column. tricine-sds page revealed the presence of a band with an estimated molecular mass of 6.3 kda. the zymogram clearly linked the antimicrobial activi ... | 2016 | 26786752 |
| nitric oxide-releasing polyacrylonitrile disperses biofilms formed by wound-relevant pathogenic bacteria. | to test the antimicrobial and antibiofilm properties of a nitric oxide (no)-releasing polymer against wound-relevant bacterial pathogens. | 2016 | 26784529 |
| escherichia coli and enterococcus faecalis are able to incorporate and enhance a pre-formed gardnerella vaginalis biofilm. | gardnerella vaginalis is the most frequent microorganism found in bacterial vaginosis (bv), while escherichia coli and enterococcus faecalis are amongst the most frequent pathogens found in urinary tract infections (utis). this study aimed to evaluate possible interactions between utis pathogens and g. vaginalis using an in vitro dual-species biofilm model. our results showed that dual-species biofilms reached significantly higher bacterial concentration than monospecies biofilms. moreover, visu ... | 2016 | 26782142 |
| prevalence, outcome and risk factor associated with vancomycin-resistant enterococcus faecalis and enterococcus faecium at a tertiary care hospital in northern india. | to determine the prevalence, genotype, risk factors and mortality in patients having vancomycin-resistant enterococcus faecalis (vr e. faecalis) and enterococcus faecium (vr e. faecium) infection or colonisation. | 2016 | 26776117 |
| antibacterial activity and immunomodulatory effects on a bovine mammary epithelial cell line exerted by nisin a-producing lactococcus lactis strains. | twenty-nine strains of mastitis pathogens were used to study the antibacterial activity of the cell-free supernatants (cfs) of 25 strains of lactococcus lactis ssp. lactis. out of the tested strains, only the cfs of l. lactis ll11 and sl153 were active, inhibiting and killing most of the pathogens. by means of ultra-performance liquid chromatography/high resolution mass spectrometry, they were shown to produce nisin a, a class i bacteriocin. a variable sensitivity to nisin a-containing cfs was o ... | 2016 | 26774727 |
| complete genome sequence of enterococcus faecalis strain p8-1 isolated from wild magellanic penguin (spheniscus magellanicus) feces on the south coast of brazil. | enterococcus faecalis strains have a ubiquitous nature that allows them to survive in different niches. studies involving enterococci isolated from marine animals are scarce. therefore, in this study, we report the complete genome sequence of e. faecalis strain p8-1 isolated from feces of a magellanic penguin on the south coast of brazil. | 2016 | 26769928 |
| caenorhabditis elegans susceptibility to gut enterococcus faecalis infection is associated with fat metabolism and epithelial junction integrity. | gut bacteria-host interactions have been implicated in the pathogenesis of numerous human diseases, but few mechanisms have been described. the genetically tractable nematode worm caenorhabditis elegans can be infected with pathogenic bacteria, such as the human gut commensal enterococcus faecalis, via feeding, making it a good model for studying these interactions. | 2016 | 26769134 |
| fumarate reductase-producing enterococci reduce methane production in rumen fermentation in vitro. | biotic agents such as fumarate-reducing bacteria can be used for controlling methane (ch4) production in the rumen. fumarate-reducing bacteria convert fumarate to succinate by fumarate reductase, ultimately leading to the production of propionate. fumarate-reducing bacteria in the genus enterococcus were isolated from rumen fluid samples from slaughtered korean native goats. the enterococci were identified as enterococcus faecalis srod5 and e. faecium srod by phylogenetic analyses of 16s rrna ge ... | 2016 | 26767574 |
| the efficacy of photon-initiated photoacoustic streaming and sonic-activated irrigation combined with qmix solution or sodium hypochlorite against intracanal e. faecalis biofilm. | the aim of the study was to assess the antibacterial efficacy of photon-initiated photoacoustic streaming (pips) using an er:yag laser and sonic-activated irrigation combined with qmix irrigant or sodium hypochlorite against enterococcus faecalis intracanal biofilm. root canals of 91 human extracted single-canal teeth were instrumented, sterilized, contaminated with e. faecalis and incubated for 15 days. the infected teeth were then randomly distributed into six experimental groups: g1: pips/er: ... | 2016 | 26754179 |
| establishment and analysis of in vitro biomass from salvia corrugata vahl. and evaluation of antimicrobial activity. | demethylfruticuline a and fruticuline a, the most abundant compounds from the surface extract of salvia corrugata vahl., have shown antibacterial, antitumor and cytotoxic activities. in order to obtain these icetexane diterpenes from in vitro cultures of s. corrugata, protocols were developed for callus production, micropropagation and shoot regeneration. analysis of the regenerated shoots showed the presence of both icetexanes, micropropagated plants contained only fruticuline a, while the call ... | 2016 | 26753532 |
| antimicrobial effect of an oxazolidinone, lantibiotic and calcium hydroxide against enterococcus faecalis biofilm: an in vitro study. | the aim was to evaluate and compare the antimicrobial efficacy of an oxazolidinone (linezolid [lz]), lantibiotic (nisin), and calcium hydroxide against enterococcus faecalis biofilm formed on tooth substrate after 2 and 7 days. | 2017 | 26752879 |
| antimicrobial efficacy of different root canal sealers by using real-time polymerase chain reaction: an ex vivo study. | root canal sealers help to minimize leakage, provides antimicrobial activity to reduce the possibility of residual bacteria, and to resolve periapical lesion. | 2017 | 26752843 |
| asymptomatic bacteriuria in clinical urological practice: preoperative control of bacteriuria and management of recurrent uti. | asymptomatic bacteriuria (abu) is a common clinical condition that often leads to unnecessary antimicrobial use. the reduction of antibiotic overuse for abu is consequently an important issue for antimicrobial stewardship and to reduce the emergence of multidrug resistant strains. there are two issues in everyday urological practice that require special attention: the role of abu in pre-operative prophylaxis and in women affected by recurrent urinary tract infections (rutis). nowadays, this is t ... | 2016 | 26742080 |
| early gut colonization of preterm infants: effect of enteral feeding tubes. | the aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates. | 2016 | 26741949 |
| enterococcus faecalis as multidrug resistance strains in clinical isolates in imam reza hospital in kermanshah, iran. | the current study aimed to investigate the prevalence of vancomycin-resistant enterococcus in e. faecalis and e. faecium and antimicrobial susceptibility patterns, then dominant genes responsible for vancomycin resistance were determined. for this propose, 180 clinical isolates of enterococcus were subjected for identification and antibiotic susceptibility assay. then, the gene responsible vancomycin resistant strains were determined. the results demonstrated the e. faecalis as a dominant entero ... | 2015 | 26738400 |
| in silico driven design and synthesis of rhodanine derivatives as novel antibacterials targeting the enoyl reductase inha. | here, we report on the design, synthesis, and biological evaluation of 4-thiazolidinone (rhodanine) derivatives targeting mycobacterial tuberculosis (mtb) trans-2-enoyl-acyl carrier protein reductase (inha). compounds having bulky aromatic substituents at position 5 and a tryptophan residue at position n-3 of the rhodanine ring were the most active against inha, with ic50 values ranging from 2.7 to 30 μm. the experimental data showed consistent correlations with computational studies. their anti ... | 2016 | 26730986 |
| diversity and evolution of the tn5801-tet(m)-like integrative and conjugative elements among enterococcus, streptococcus, and staphylococcus. | this work describes the diversity and evolution of tn5801 among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. we also examined 610 isolates of enterococcus (from 10 countries, 1987 to 2010) for the presence of this and other known ctn-tet(m) elements due to the scarcity of data about tn5801 among enterococci. genome location (by iceu-i-pulsed-field gel electrophoresis [pfge] hybridization/integration sit ... | 2016 | 26729505 |
| chromosomally and extrachromosomally mediated high-level gentamicin resistance in streptococcus agalactiae. | streptococcus agalactiae (group b streptococcus [gbs]) is a leading cause of sepsis in neonates. the rate of invasive gbs disease in nonpregnant adults also continues to climb. aminoglycosides alone have little or no effect on gbs, but synergistic killing with penicillin has been shown in vitro. high-level gentamicin resistance (hlgr) in gbs isolates, however, leads to the loss of a synergistic effect. we therefore performed a multicenter study to determine the frequency of hlgr gbs isolates and ... | 2016 | 26729498 |
| antibiotic susceptibility profiles of dairy leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrix. | in spite of a global concern on the transfer of antibiotic resistances (ar) via the food chain, limited information exists on this issue in species of leuconostoc and weissella, adjunct cultures used as aroma producers in fermented foods. in this work, the minimum inhibitory concentration was determined for 16 antibiotics in 34 strains of dairy origin, belonging to leuconostoc mesenteroides (18), leuconostoc citreum (11), leuconostoc lactis (2), weissella hellenica (2), and leuconostoc carnosum ... | 2016 | 26726815 |
| gentamicin induces efaa expression and biofilm formation in enterococcus faecalis. | enterococci have been ranked among the leading causes of nosocomial bacteremia and urinary tract infection. this study aimed to investigate the effect of ampicillin, vancomycin, gentamicin and ceftizoxime on biofilm formation and gene expression of colonization factors on enterococcus faecalis. twelve clinical isolates of e. faecalis were used to investigate the effect of antibiotics on biofilm formation and gene expression of efaa, asa1, ebpa, esp and ace. flow system assay and microtiter plate ... | 2016 | 26724739 |
| effectiveness of edta and modified salt solution to detach and kill cells from enterococcus faecalis biofilm. | disruption of the matrix of endodontic biofilms will aid in their removal from a root canal. therefore, the aim of this study was to investigate the efficacy of edta and a modified salt solution (mss) to detach bacteria from biofilms. | 2016 | 26723483 |
| first identification of tn916-like element in industrial strains of lactobacillus vini that spread the tet-m resistance gene. | the open process used to ferment sugar cane juice or molasses to produce ethanol fuel is prone to contamination by bacterial cells of different species, in particular lactobacilli. the situation can be exacerbated by the emergence of resistant cells to industrial antibiotics that are normally used to combat this contamination. in this work, two lactobacillus vini isolates from ethanol distilleries were identified and found to be resistant to doxycycline, a tetracycline derivative, although sensi ... | 2016 | 26722009 |
| discordance between perioperative antibiotic prophylaxis and wound infection cultures in patients undergoing pancreaticoduodenectomy. | wound infections after pancreaticoduodenectomy (pd) are common. the standard antibiotic prophylaxis given to prevent the infections is often a cephalosporin. however, this decision is rarely guided by microbiology data pertinent to pd, particularly in patients with biliary stents. | 2016 | 26720272 |
| synthesis and biological evaluation of 2-aminobenzothiazole and benzimidazole analogs based on the clathrodin structure. | a series of 2-aminobenzothiazole and benzimidazole analogs based on the clathrodin scaffold was synthesized and investigated for their antimicrobial and antiproliferative activities as well as for their effects in hepatitis c virus (hcv) replicon model. compound 7, derived from 2-aminobenzothiazole, exhibited moderate antimicrobial activity only against the gram-positive bacterium, enterococcus faecalis. in the antiviral assay, compounds 4d and 7 were found to suppress the hcv replicon by >70%, ... | 2016 | 26709468 |
| human health risks associated with antimicrobial-resistant enterococci and staphylococcus aureus on poultry meat. | enterococci and staphylococci are frequent contaminants on poultry meat. enterococcus faecalis, enterococcus faecium and staphylococcus aureus are also well-known aetiological agents of a wide variety of infections resulting in major healthcare costs. this review provides an overview of the human health risks associated with the occurrence of these opportunistic human pathogens on poultry meat with particular focus on the risk of food-borne transmission of antimicrobial resistance. in the absenc ... | 2016 | 26706616 |
| [a case of pyoderma gangrenosum of the penis difficult to distinguish from fournier gangrene]. | here, we report a case of pyoderma gangrenosum of the penis which was difficult to distinguish from fournier gangrene. the patient was a 54-year-old male who was aware of redness and swelling of the glanspenis for 1 month prior to a consultation at our department. although he was diagnosed with herpes and treated at a nearby hospital, his symptoms did not improve. subsequently, the patient visited our department following the onset of pain and fever. during his initial consultation, he had a fev ... | 2015 | 26699892 |
| evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of enterococcus faecalis. | background and aims. triple antibiotic paste (tap) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. the aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of tap at 1-, 2-, 3-, and 4-week intervals on mature enterococcus faecalis biofilm. materials and methods. a total of 287 extracted one-rooted human central incisors were infected with e. faecalis atcc 29212 after removing the crown and preparation ... | 2015 | 26697145 |
| the capability of tyramine production and correlation between phenotypic and genetic characteristics of enterococcus faecium and enterococcus faecalis strains. | the aim of this study was to investigate the diversity of tyramine production capability of four enterococcus strains in buffered systems in relation to their genetic characteristics and environmental conditions. cells of the strains enterococcus faecalis ef37 and atcc 29212, and e. faecium fc12 and fc643 were re-suspended in phosphate/citrate buffers with different ph, nacl concentration and incubation temperature. at intervals, cell viability and tyramine production were assessed by plate coun ... | 2015 | 26696981 |
| olive mill waste extracts: polyphenols content, antioxidant, and antimicrobial activities. | natural polyphenols extracts have been usually associated with great bioactive properties. in this work, we investigated in vitro antioxidant and antimicrobial potential of the phenolic olive mill wastewater extracts (owwe) and the olive cake extracts (oce). using the folin ciocalteux method, owwe contained higher total phenol content compared to oce (8.90 ± 0.728 g/l versus 0.95 ± 0.017 mg/g). the phenolic compounds identification was carried out with a performance liquid chromatograph coupled ... | 2015 | 26693221 |
| the bactericidal effect of 2780 and 940 nm laser irradiation on enterococcus faecalis in bovine root dentin slices of different thicknesses. | the aim of this in vitro study was to investigate the antibacterial effect of the dual wavelength (2780 nm er,cr:ysgg and 940 nm diode) laser in elimination of enterococcus faecalis in comparison with a 2780 nm er,cr:ysgg laser alone. | 2016 | 26685038 |
| tigecycline resistance in clinical isolates of enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(l) and tet(m). | tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including vre and mrsa. the german national reference centre for staphylococci and enterococci has received 73 tigecycline-resistant enterococcus faecium and enterococcus faecalis isolates in recent years. the precise mechanism of how enterococci become resistant to tigecycline remains undetermined. this study documents an analysis of the role of efflux pumps in tigecycline resistance in c ... | 2016 | 26682961 |
| enterococcus faecalis septicemia and vertebral osteomyelitis after transrectal ultrasound guided biopsy of the prostate. | transrectal ultrasound guided prostate biopsy (trus) has rarely been associated with disseminated infection, yet the occurrence appears to be increasing. resistance to fluoroquinolones, the most commonly used prophylaxis, is one of the likely causes, with escherichia coli being the most commonly reported cause of these infections. herein we present what is, to our knowledge, the first case of enterococcus faecalis septicemia and vertebral osteomyelitis after trus. previously reported cases of th ... | 2015 | 26682075 |
| genomic sequence of a clinical vancomycin-resistant reference strain, enterococcus faecalis atcc 51299. | in this paper, we present a draft genome sequence of a quality control reference strain, enterococcus faecalis atcc 51299 (multilocus sequencing type [mlst] st6), which is sensitive to teicoplanin but resistant to vancomycin. it is used in an agar screening test for streptomycin, gentamicin, and vancomycin resistance and the resistance marker vanb. | 2015 | 26679597 |
| microbial evolution of traditional mountain cheese and characterization of early fermentation cocci for selection of autochtonous dairy starter strains. | the microbial population of traditional mountain (tm) cheese was investigated and characterized for the selection of cocci suitable for developing new starter cultures. samples of milk, curd and cheese at different ripening times were enumerated in selective culture media and 640 colonies were isolated from curd and cheese after 24 h of ripening. the lactic acid bacteria (lab) isolated from m17 were clustered into 231 biotypes by rapd-pcr analysis and identified as lactococcus lactis, streptococ ... | 2016 | 26678135 |
| control of shigatoxin-producing escherichia coli in cheese by dairy bacterial strains. | bio-preservation could be a valuable way to control shigatoxin-producing escherichia coli (stec) in cheese. to this end, 41 strains were screened for their inhibitory potential on model cheese curd and on pasteurized and raw milk uncooked pressed cheeses. strains of lactococcus lactis, lactococcus garvieae, leuconostoc pseudomesenteroides, leuconostoc citreum, lactobacillus sp, carnobacterium mobile, enterococcus faecalis, enterococcus faecium, macrococcus caseolyticus and hafnia alvei reduced s ... | 2016 | 26678131 |
| substantivity of ag-ca-si mesoporous nanoparticles on dentin and its ability to inhibit enterococcus faecalis. | the main purpose of this study was to investigate the substantivity of ag-ca-si mesoporous nanoparticles (ag-mcsns) on dentin and its residual antibacterial effects against enterococcus faecalis. ag-mcsns were fabricated and characterized, ion release profile and ph were tested, and the ability to inhibit planktonic e. faecalis as well as the cytotoxicity was evaluated. dentin slices were medicated with ca(oh)2 paste, 2 % chlorhexidine gel and ag-mcsns paste for 7 days and then irrigated. dentin ... | 2016 | 26676862 |
| the effect of addition of an eps degrading enzyme with and without detergent to 2% chlorhexidine on disruption of enterococcus faecalis biofilm: a confocal laser scanning microscopic study. | enterococcus faecalis is one of the most commonly occurring organisms retrieved from root canal treated teeth that show refractory apical periodontitis. though it is well known that the ability of e. faecalis to form a matrix-encased biofilm contributes to its pathogenicity, the role of extracellular dextran and dna in biofilm formation and its effect on the susceptibility of the biofilm to chlorhexidine remains poorly understood. it was hypothesized that the addition of an extracellular polymer ... | 2015 | 26675655 |
| cleistochlamys kirkii chemical constituents: antibacterial activity and synergistic effects against resistant staphylococcus aureus strains. | cleistochlamys kirkii (benth) oliv., (annonaceae) is a medicinal plant traditionally used in mozambique to treat infectious diseases. | 2016 | 26674158 |
| an in vitro evaluation of antimicrobial activity of five herbal extracts and comparison of their activity with 2.5% sodium hypochlorite against enterococcus faecalis. | sodium hypochlorite is the most widely used irrigant in endodontic practice, but it has various disadvantages. literature has shown that herbal products such as propolis, azadirachta indica (ai), triphala, curcuma longa, and morinda citrifolia (mc) possess good antimicrobial properties and thus can be used as potential endodontic irrigants. | 2015 | 26672425 |
| anti-microbial efficacy of allium sativum extract against enterococcus faecalis biofilm and its penetration into the root dentin: an in vitro study. | sodium hypochlorite (naocl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effective led to this study. | 2015 | 26672416 |
| effects of salinity and temperature on inactivation and repair potential of enterococcus faecalis following medium- and low-pressure ultraviolet irradiation. | to investigate the medium-pressure (mp) and low-pressure (lp) ultraviolet (uv) susceptibility and the repair potential of enterococcus faecalis (dsm 20478) after uv treatment. | 2016 | 26669257 |
| reduction of viable enterococcus faecalis in human radicular dentin treated with 1% cetrimide and conventional intracanal medicaments. | the purpose of this study was to evaluate the viability of e. faecalis after a 14-day exposure to 1% cetrimide (cet), triantibiotic paste (i.e., metronidazole, minocycline, and ciprofloxacin), 2% chlorhexidine (chx) gel, and calcium hydroxide (ca[oh]2 ) in an infected dentine model. | 2016 | 26666992 |
| hydrothermally treated chitosan hydrogel loaded with copper and zinc particles as a potential micronutrient-based antimicrobial feed additive. | large-scale use of antibiotics in food animal farms as growth promoters is considered as one of the driving factors behind increasing incidence of microbial resistance. several alternatives are under investigation to reduce the amount of total antibiotics used in order to avoid any potential transmission of drug resistant microbes to humans through food chain. copper sulfate and zinc oxide salts are used as feed supplement as they exhibit antimicrobial properties in addition to being micronutrie ... | 2015 | 26664989 |
| the opportunistic pathogen enterococcus faecalis resists phagosome acidification and autophagy to promote intracellular survival in macrophages. | while many strains of enterococcus faecalis have been reported to be capable of surviving within macrophages for extended periods, the exact mechanisms involved are largely unknown. in this study, we found that after phagocytosis by macrophages, enterococci-containing vacuoles resist acidification, and e. faecalis is resistant to low ph. ultrastructural examination of the enterococci-containing vacuole by transmission electron microscopy revealed a single membrane envelope, with no evidence of t ... | 2016 | 26663775 |
| gram-positive cocci in dutch icus with and without selective decontamination of the oropharyngeal and digestive tract: a retrospective database analysis. | the objective of this study was to determine time trends in the rate of gram-positive cocci in 42 dutch icus that continuously used or did not use selective oropharyngeal decontamination (sod) or selective decontamination of the digestive tract (sdd) and icus that introduced sod/sdd. | 2016 | 26661393 |
| degree of bacterial microleakage at the implant-abutment junction in cone morse tapered implants under loaded and unloaded conditions. | different results have been reported on the internal colonization of cone morse connections under in vitro dynamic loading. the aim of the present in vitro study was to evaluate the bacterial leakage in cone morse implant-abutment connections, both under loaded and unloaded conditions. | 2015 | 26660625 |