| diversity of enterococcus faecalis genotypes from multiple oral sites associated with endodontic failure using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction. | the aim of this study was to evaluate the diversity and similarity of enterococcus faecalis genotype isolates from multiple oral sites using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction (ap-pcr). | 2017 | 28131414 |
| [update on antibiotic resistance in gram-positive bacteria]. | antimicrobial resistance among gram-positive bacteria, especially in staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and streptococcus pneumoniae, is a serious threat to public health. these microorganisms have multiple resistance mechanisms to agents currently used in clinical practice. many of these resistance mechanisms are common to all 4 of these bacterial species, but other mechanisms seem to be more specific. the prevalence and dissemination of these mechanisms varies ... | 2017 | 28129816 |
| antimicrobial activity of endodontic medicaments and vehicles using agar well diffusion method on facultative and obligate anaerobes. | the aim of this study was to determine the relative antimicrobial effectiveness of these endodontic medicaments and various vehicles using an agar well diffusion assay. | 2017 | 28127166 |
| a comparison of antibacterial and antibiofilm efficacy of phenothiazinium dyes between gram positive and gram negative bacterial biofilm. | antimicrobial photodynamic therapy (apdt) is a process that generates reactive oxygen species (ros) in presence of photosensitizer, visible light and oxygen which destroys the bacterial cells. we investigated the photoinactivation efficiency of phenothiazinium dyes and the effect of ros generation on gram positive and gram negative bacterial cell as well as on biofilm. | 2017 | 28119141 |
| growth, biogenic amine production and tyrdc transcription of enterococcus faecalis in synthetic medium containing defined amino acid concentrations. | the tyraminogenic potential of the strains enterococcus faecalis ef37 and atcc 29212 was investigated in a synthetic medium containing defined amounts of tyrosine and phenylalanine at different temperatures. | 2017 | 28117533 |
| catheter-associated urinary tract infection (cauti) after term cesarean delivery: incidence and risk factors at a multi-center academic institution. | the purpose of this study is to identify the rate of catheter-associated urinary tract infection (cauti) after cesarean delivery (cd) and to determine if any factors increase risk of infection. | 2017 | 28114875 |
| comparative efficacies of daptomycin, vancomycin, and linezolid in experimental enterococcal peritonitis. | enterococci have become increasingly important pathogens for nosocomial infection (e.g. bacteremia, intra-abdominal infection, endocarditis, etc.), related to their intrinsic resistance to many antibiotics. although the in vitro susceptibility of daptomycin (dap) against enterococci is well established, the food and drug administration has only approved its use for complicated skin and skin structure infections induced by enterococcus faecalis. in this study we evaluated the potential therapeuti ... | 2017 | 28108098 |
| antibacterial activity, computational analysis and host toxicity study of thymol-sulfonamide conjugates. | methicillin-resistant staphylococcus aureus (mrsa) and vancomycin resistant enterococcus faecalis (vre) are notorious pathogenic multidrug resistant (mdr) bacteria in both hospital and community sectors, and today the first antibacterial drug sulfamethoxazole is ineffective. the monoterpene phenol, thymol was conjugated with seven sulfa drug derivatives individually, adopting the dye-azo synthesis protocol, and conjugates were characterized using spectral analysis techniques such as, uv, ftir, m ... | 2017 | 28107695 |
| draft genome sequence of an enterococcus faecalis atcc 19433 siphovirus isolated from raw domestic sewage. | we previously isolated and characterized an enterococcus faecalis atcc 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. here, we report the draft genome sequence of this bacteriophage. | 2017 | 28104647 |
| model systems for the study of enterococcal colonization and infection. | enterococcus faecalis and enterococcus faecium are common inhabitants of the human gastrointestinal tract, as well as frequent opportunistic pathogens. enterococci cause a range of infections including, most frequently, infections of the urinary tract, catheterized urinary tract, bloodstream, wounds and surgical sites, and heart valves in endocarditis. enterococcal infections are often biofilm-associated, polymicrobial in nature, and resistant to antibiotics of last resort. understanding enteroc ... | 2017 | 28102784 |
| interspecies transfer of vancomycin, erythromycin and tetracycline resistance among enterococcus species recovered from agrarian sources. | enterococci are now well recognised for their ability to transfer antibiotic resistance and for their association with nosocomial infections, but less is known regarding their relevance in the wider environment. enterococcus faecalis and enterococcus faecium were isolated from a range of agrarian associated sources (low-flow water, septic tank, poultry litter, high flow water, slurry/soil) and were assessed for latent ability to transfer antimicrobial resistance. | 2017 | 28100194 |
| enterococcus faecalis causing delayed spondylodiscitis in a case with retained intraspinal bullet. | delayed presentations have been reported following gunshot wounds (gsw) with retained intraspinal bullets due to migration of projectile or lead intoxication. we report on the rare occurrence of delayed pyogenic spondylodiscitis and neurological dysfunction following injury from low velocity gsw to the spine with a retained projectile. a 55-year-old male presented 4 months following gsw to the abdomen which resulted in colonic injury and l5 fracture. the patient was treated initially with ileo-t ... | 2016 | 28097252 |
| antibacterial efficacy of ah plus and ah26 sealers mixed with amoxicillin, triple antibiotic paste and nanosilver. | background. elimination of bacteria from the root canal system is one of the aims of endodontic treatment; hence the incorporation of antibiotics into sealers can increase their antimicrobial efficacy. the aim of the present study was to determine the in vitro antimicrobial effects of ah26 and ah plus sealers mixed with amoxicillin, triple antibiotic paste and nanosilver on enterococcus faecalis. methods. in this experiment, amoxicillin, triple antibiotic paste and nanosilver powder were added a ... | 2016 | 28096947 |
| pharmacodynamics of ceftaroline plus ampicillin against enterococcus faecalis in an in vitro pharmacokinetic/pharmacodynamic model of simulated endocardial vegetations. | the combination of ampicillin plus ceftaroline has been suggested to be more reliably synergistic against enterococcus faecalis than ampicillin plus ceftriaxone using time-kill methods. the purpose of this study was to determine if this trend persists in a two-compartment model of simulated endocardial vegetations (sev) using clinically relevant pharmacokinetic exposures of these antimicrobials. three clinically derived e. faecalis strains were included in the study. the mics of study antimicrob ... | 2017 | 28096164 |
| structural proteins of enterococcus faecalis bacteriophage ϕef11. | ϕef11, a temperate siphoviridae bacteriophage, was isolated by induction from a root canal isolate of enterococcus faecalis. sequence analysis suggested that the ϕef11 genome included a contiguous 8 gene module whose function was related to head structure assembly and another module of 10 contiguous genes whose products were responsible for tail structure assembly. sds-page analysis of virions of a ϕef11 derivative revealed 11 well-resolved protein bands. to unify the deduced functional gene ass ... | 2016 | 28090386 |
| eradication of enterococcus faecalis biofilms on human dentin. | objectives: this work assesses different methods to interfere with enterococcus faecalis biofilms formed on human dentin slabs. methods: first, methods are presented that select for small molecule inhibitors of biofilm targets using multi-well polystyrene biofilm plates. next, we establish methodologies to study and interfere with biofilm formation on a medically relevant model, whereby biofilms are grown on human root dentin slabs. results: non-conventional d-amino acid (d-leucine) can efficien ... | 2016 | 28082955 |
| genome sequence of enterococcus faecalis strain cg_e. | enterococcus faecalis cg_e is a gram-positive, lactic acid-producing coccus. the draft genome of e. faecalis strain cg_e comprises 2,969,881 bp and exhibits a g+c content of 37.34%. the genome encodes 2,848 predicted protein-encoding and 97 rna genes. | 2017 | 28082508 |
| [early results of adjuvant topical treatment of recurrent osteomyelitis with absorbable antibiotic carriers]. | background treatment of musculoskeletal infections principally consists of radical surgical debridement and systemic administration of antibiotics. additional local antibiotic therapy is not yet generally established, and lacks evidence-based proof of efficacy. nonetheless, there are a variety of practical approaches, as most specialised departments are unwilling to forego this option. the established polymethylmetacrylate (pmma) carrier system has a number of practical disadvantages. this has l ... | 2017 | 28081583 |
| characterization of multi-drug resistant enterococcus faecalis isolated from cephalic recording chambers in research macaques (macaca spp.). | nonhuman primates are commonly used for cognitive neuroscience research and often surgically implanted with cephalic recording chambers for electrophysiological recording. aerobic bacterial cultures from 25 macaques identified 72 bacterial isolates, including 15 enterococcus faecalis isolates. the e. faecalis isolates displayed multi-drug resistant phenotypes, with resistance to ciprofloxacin, enrofloxacin, trimethoprim-sulfamethoxazole, tetracycline, chloramphenicol, bacitracin, and erythromyci ... | 2017 | 28081148 |
| ozone therapy as an adjuvant for endondontic protocols: microbiological - ex vivo study and citotoxicity analyses. | this study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with pseudomonas aeruginosa, enterococcus faecalis, and staphylococcus aureus using a mono-species biofilm model. parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. material and methods: one hundred and eighty single-root teeth were contaminated with a mono-species biofilm of enterococcus faecalis, pseudomonas aeruginosa, and staphylococcus aureus. groups we ... | 2017 | 28076466 |
| effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment. | the antimicrobial effect of ultrasonic agitation of calcium hydroxide (ch) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (clsm) and microbiological culture. | 2017 | 28076462 |
| a novel phosphodiesterase of the gdpp family modulates cyclic di-amp levels in response to cell membrane stress in daptomycin-resistant enterococci. | substitutions in the liafsr membrane stress pathway are frequently associated with the emergence of antimicrobial peptide resistance in both enterococcus faecalis and enterococcus faecium cyclic di-amp (c-di-amp) is an important signal molecule that affects many aspects of bacterial physiology, including stress responses. we have previously identified a mutation in a gene (designated yybt) in e. faecalis that was associated with the development of daptomycin resistance, resulting in a change at ... | 2017 | 28069645 |
| probiotic e. coli nissle 1917 biofilms on silicone substrates for bacterial interference against pathogen colonization. | bacterial interference is an alternative strategy to fight against device-associated bacterial infections. pursuing this strategy, a non-pathogenic bacterial biofilm is used as a live, protective barrier to fence off pathogen colonization. in this work, biofilms formed by probiotic escherichia coli strain nissle 1917 (ecn) are investigated for their potential for long-term bacterial interference against infections associated with silicone-based urinary catheters and indwelling catheters used in ... | 2017 | 28069496 |
| design, synthesis and antibacterial evaluation of novel pleuromutilin derivatives possessing piperazine linker. | a series of pleuromutilin derivatives bearing piperazine ring have been reported. the in vitro antibacterial activities of the synthetic derivatives against mrsa (atcc 43300), staphylococcus aureus (atcc 29213), enterococcus faecalis (atcc 29212), enterococcus faecium (atcc35667) and escherichia coli (atcc25922) were evaluated by the broth dilution method. most of the synthesized derivatives displayed potent activities. compounds 11c, 12a and 12c were found to be the most active antibacterial de ... | 2017 | 28068600 |
| the effect of diode laser on planktonic enterococcus faecalis in infected root canals in an ex vivo model. | this study examined the bactericidal effect of diode laser irradiation against intracanal enterococcus faecalis. | 2017 | 28068207 |
| bactericidal efficacy of photodynamic therapy and chitosan in root canals experimentally infected with enterococcus faecalis: an in vitro study. | to evaluate the antibacterial efficacy of photodynamic therapy (pdt) and chitosan against enterococcus faecalis and assess the possible enhancive effect of chitosan on the photosensitizer methylene blue in experimentally infected root canals of extracted human teeth in vitro. | 2017 | 28068186 |
| cinnamaldehyde inhibits staphylococcus aureus virulence factors and protects against infection in a galleria mellonella model. | bacterial resistance to the available marketed drugs has prompted the search of novel therapies; especially in regards of anti-virulence strategies that aim to make bacteria less pathogenic and/or decrease their probability to become resistant to therapy. cinnamaldehyde is widely known for its antibacterial properties through mechanisms that include the interaction of this compound with bacterial cell walls. however, only a handful of studies have addressed its effects on bacterial virulence, es ... | 2016 | 28066373 |
| genetic relatedness of faecal coliforms and enterococci bacteria isolated from water and sediments of the apies river, gauteng, south africa. | to date, the microbiological quality of river sediments and its impact on water resources are not included in the water quality monitoring assessment. therefore, the aim of this study was to establish genetic relatedness between faecal coliforms and enterococci isolated from the river water and riverbed sediments of apies river to better understand the genetic similarity of microorganisms between the sediment and water phases. indicator bacteria were subjected to a molecular study, which consist ... | 2017 | 28063147 |
| fosfomycin: mechanism and resistance. | fosfomycin, a natural product antibiotic, has been in use for >20 years in spain, germany, france, japan, brazil, and south africa for urinary tract infections (utis) and other indications and was registered in the united states for the oral treatment of uncomplicated utis because of enterococcus faecalis and escherichia coli in 1996. it has a broad spectrum, is bactericidal, has very low toxicity, and acts as a time-dependent inhibitor of the mura enzyme, which catalyzes the first committed ste ... | 2017 | 28062557 |
| critical role of tedizolid in the treatment of acute bacterial skin and skin structure infections. | tedizolid phosphate has high activity against the gram-positive microorganisms mainly involved in acute bacterial skin and skin structure infections, such as strains of staphylococcus aureus (including methicillin-resistant s. aureus strains and methicillin-sensitive s. aureus strains), streptococcus pyogenes, streptococcus agalactiae, the streptococcus anginosus group, and enterococcus faecalis, including those with some mechanism of resistance limiting the use of linezolid. the area under the ... | 2017 | 28053508 |
| isolation of rhamnolipids-producing cultures from faeces: influence of interspecies communication on the yield of rhamnolipid congeners. | the aim of this study was to evaluate the ability of bacterial cultures isolated from cattle, poultry or pig faeces and manure to produce rhamnolipids, as well as to investigate the influence of interspecies communication on possible quantitative differences in the production of rhamnolipid congeners. initial screening methods (oil spreading, drop collapse, haemolytic activity and emulsification activity) showed that approximately 36% of the 51 isolated cultures exhibited the ability to produce ... | 2017 | 28043869 |
| quantification of the d-ala-d-lac-terminated peptidoglycan structure in vancomycin-resistant enterococcus faecalis using a combined solid-state nuclear magnetic resonance and mass spectrometry analysis. | induction of vancomycin resistance in vancomycin-resistant enterococci (vre) involves replacement of the d-ala-d-ala terminus of peptidoglycan (pg) stems with d-ala-d-lac, dramatically reducing the binding affinity of vancomycin for lipid ii. effects from vancomycin resistance induction in enterococcus faecalis (atcc 51299) were characterized using a combined solid-state nuclear magnetic resonance (nmr) and liquid chromatography-mass spectrometry (lc-ms) analysis. solid-state nmr directly measur ... | 2017 | 28040891 |
| a comparison between effect of photodynamic therapy by led and calcium hydroxide therapy for root canal disinfection against enterococcus faecalis: a randomized controlled trial. | insufficient root canal disinfection is one of the main reasons for persistent periapical pathology. photodynamic therapy (pdt) has been proven effective in disinfecting infected root canals. the aim of this study was to evaluate the antimicrobial effect of photo activated disinfection (pad) when using toluidine blue as photosensitizer and a led lamp after the conventional treatment, and comparing it with calcium hydroxide therapy in vivo. | 2017 | 28040502 |
| novel lnu(g) gene conferring resistance to lincomycin by nucleotidylation, located on tn6260 from enterococcus faecalis e531. | to identify a novel putative lincosamide resistance gene determinant in a swine enterococcus faecalis e531 exhibiting a lincosamide resistance/macrolide susceptibility (l r m s ) phenotype and to determine its location and genetic environment. | 2017 | 28039271 |
| antimicrobial resistance of selected invasive bacteria in a tertiary care center: results of a prospective surveillance study. | we aimed to report the distribution and resistance patterns of eight invasive clinically relevant bacteria surveyed in the clinical center of serbia (ccs) in belgrade. | 2016 | 28036312 |
| in vitro and ex vivo microbial leakage assessment in endodontics: a literature review. | the aim of this study was to perform a literature review of published in-vitro and ex-vivo studies, which evaluated microbial leakage in endodontics in the past 10 years. a comprehensive electronic literature search was carried out in pubmed database for english articles published from 2005 to 2016 using the keywords "endodontics," "in vitro," "ex vivo," "microbial leakage," "microbial penetration," "saliva," "enterococcus faecalis," "e. faecalis," "endodontic sealers," "temporary filling materi ... | 2017 | 28032041 |
| effect of liquid crystalline systems containing antimicrobial compounds on infectious skin bacteria. | this study aimed (i) to prepare liquid crystalline systems (lcs) of glyceryl monooleate (gmo) and water containing antibacterial compounds and (ii) to evaluate their potential as drug delivery systems for topical treatment of bacterial infections. therefore, lcs containing cpc (cetylpyridinium chloride) (lcs/cpc) and phmb (poly(hexamethylene biguanide) hydrochloride) (lcs/phmb) were prepared and the liquid crystalline phases were identified by polarizing light microscopy 24 h and 7 days after pr ... | 2016 | 28028794 |
| the use of different irrigation techniques to decrease bacterial loads in healthy and diabetic patients with asymptomatic apical periodontitis. | diabetes mellitus is a multisystem disease which weakens the human's immunity. subsequently, it worsens the sequelae of apical periodontitis by raising a fierce bacterial trait due to the impaired host response. | 2016 | 28028421 |
| entrococcus faecalis elimination in root canals using silver nanoparticles, photodynamic therapy, diode laser, or laser-activated nanoparticles: an in vitro study. | the aim of this study was to compare the efficacy of silver nanoparticles (agnps), an 810-nm diode laser (dl), conventional photodynamic therapy (pdt) with the use of indocyanine green (icg) photosensitizer, and modified pdt with the use of agnps for the disinfection of root canals inoculated with enterococcus faecalis. | 2017 | 28027821 |
| direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system. | we investigated the direct and indirect (residual) antibacterial effects of various concentrations of triple antibiotic paste (tap) loaded into a methylcellulose system. enterococcus faecalis (e. faecalis) was grown on sterilized dentin blocks (n = 60) and treated with clinically used tap (1,000 mg/ml), low concentrations of methylcellulose-based tap (100, 10, and 1 mg/ml), placebo paste, or 1.5% naocl (n = 10). the pastes were then removed, and biofilm disruption assays were performed. addition ... | 2016 | 28025443 |
| fournier's gangrene during acth therapy. | fournier's gangrene is an infectious necrotizing fasciitis of the perineal, genital, or perianal regions and is uncommon in children. adrenocorticotropic hormone (acth) is effective for the treatment of infantile spasms; however, suppression of immune function is one of the major adverse effects of this approach. we encountered a 2-month-old boy with infantile spasms that had been treated with acth and had developed complicating fournier's gangrene. strangulation of a right inguinal hernia was o ... | 2017 | 28007393 |
| detection of linezolid resistance due to the optra gene in enterococcus faecalis from poultry meat from the american continent (colombia). | three enterococcus isolates obtained from retail chicken collected in 2010-11 as part of the colombian integrated program for antimicrobial resistance surveillance (coipars) showed reduced susceptibility towards linezolid (mic 8 mg/l). | 2017 | 27999039 |
| bactericidal effect of hydroxyl radicals generated by the sonolysis and photolysis of hydrogen peroxide for endodontic applications. | the aim of endodontic root canal treatment is the elimination of bacteria and their products from an infected tooth root canal. to effectively disinfect a root canal, an ultrasonic irrigation system, in which hydroxyl radicals (ho(·)) generated artificially by sonolysis of h2o2, was developed previously for endodontic applications and was demonstrated to have bactericidal efficacy against enterococcus faecalis. to improve this system, we examined the in vitro bactericidal effects of ho(·) genera ... | 2017 | 27998731 |
| rituximab, etoposide, methylprednisolone, high-dose cytarabine, and cisplatin in the treatment of secondary hemophagocytic lymphohistiocytosis with classical hodgkin lymphoma: a case report and review of the literature. | hemophagocytic lymphohistiocytosis is becoming an increasingly recognized disorder in adults. classical hodgkin lymphoma is a relatively uncommon etiology of hemophagocytic lymphohistiocytosis and may complicate treatment options. rituximab, etoposide, methylprednisolone, high-dose cytarabine, and cisplatin are discussed here as a treatment regimen. | 2016 | 27998299 |
| bromomethylthioindole inspired carbazole hybrids as promising class of anti-mrsa agents. | series of n-substituted carbazole analogues bearing an indole ring were synthesized as anti-methicillin-resistant staphylococcus aureus (mrsa) agents from a molecular hybridization approach. the representative compound 19 showed an mic = 1 μg/ml against a panel of mrsa clinical isolates as it possessed comparable in vitro activities to that of vancomycin. moreover, compound 19 also exhibited mic = 1 μg/ml activities against a recent identified z172 mrsa strain (vancomycin-intermediate and daptom ... | 2016 | 27994762 |
| a novel combination approach of human polyclonal ivig and antibiotics against multidrug-resistant gram-positive bacteria. | gram-positive bacteria, especially methicillin-resistant staphylococcus aureus (mrsa) and enterococci, have shown a remarkable ability to develop resistance to antimicrobial agents. | 2016 | 27994476 |
| evaluation of enterococcus faecalis adhesion, penetration, and method to prevent the penetration of enterococcus faecalis into root cementum: confocal laser scanning microscope and scanning electron microscope analysis. | to ascertain the role of enterococcus faecalis in persistent infection and a possible method to prevent the penetration of e. faecalis into root cementum. | 2017 | 27994316 |
| pptab exports rgg quorum-sensing peptides in streptococcus. | a transposon mutagenesis screen designed to identify mutants that were defective in peptide-pheromone signaling of the rgg2/rgg3 pathway in streptococcus pyogenes generated insertions in sixteen loci displaying diminished reporter activity. fourteen unique transposon insertions were mapped to pptab, an abc-type transporter recently described to export sex pheromones of enterococcus faecalis. consistent with an idea that pptab exports signaling peptides, the pheromones known as shps (short hydrop ... | 2016 | 27992504 |
| enterococcus species diversity in fecal samples of wild marine species as determined by real-time pcr. | analyses using culture-independent molecular techniques have improved our understanding of microbial composition. the aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative pcr. seven enterococcus species were examined in fecal dna of south american fur seals (arctocephalus australis), subantarctic fur seals (arctocephalus tropicalis), green turtles (chelonia mydas), magellanic penguins (spheniscus magellanicus), snowy-crown ... | 2017 | 27991828 |
| vana-positive multi-drug-resistant enterococcus spp. isolated from surfaces of a us hospital laundry facility. | enterococcus spp. are a normal part of the gastrointestinal tract of humans and animals. they are also important pathogens, being responsible for 14% of us nosocomial infections from 2007 to 2010. | 2017 | 27989378 |
| broad-range 16s rdna pcr on heart valves in infective endocarditis. | infective endocarditis (ie) is diagnosed by blood and/or resected valve cultivation and echocardiographic findings, as defined by the duke criteria. unfortunately, cultures may be negative due to prior antibiotic therapy or fastidious or slow-growing microorganisms. the study aim was to investigate the value of the broad-range polymerase chain reaction (pcr) in addition to blood and valve culture for the detection of causative microorganisms. | 2016 | 27989071 |
| ofloxacin loaded gellan/pva nanofibers - synthesis, characterization and evaluation of their gastroretentive/mucoadhesive drug delivery potential. | the purpose of this investigation is to formulate a gastroretentive sustained drug release system for ofloxacin to improve its retention time, pharmacological activity, bioavailability and therapeutic efficacy in the stomach. ofloxacin loaded gellan/poly vinyl alcohol (pva) nanofibers were fabricated using a simple and versatile electrospinning technique. the fabricated nanofibers were evaluated for percent drug encapsulation efficiency and in vitro drug release in simulated gastric medium (ph1. ... | 2017 | 27987752 |
| culture methods impact recovery of antibiotic-resistant enterococci including enterococcus cecorum from pre- and postharvest chicken. | pathogenic strains of enterococcus cecorum (ec) expressing multidrug resistance have emerged. in national antimicrobial resistance monitoring system (narms) data, ec is rarely recovered from chickens. two narms methodologies (fda and usda) were compared with standard culture (sc) techniques for recovery of ec. narms methods failed to detect ec in 58 caecal samples, 20 chicken breast or six whole broiler samples. ec was recovered from 1 of 38 (2·6%) and 2 of 38 (5·2%) preharvest spinal lesions (u ... | 2017 | 27987523 |
| integrated multivariate analysis with nondetects for the development of human sewage source-tracking tools using bacteriophages of enterococcus faecalis. | we developed sewage-specific microbial source tracking (mst) tools using enterococci bacteriophages and evaluated their performance with univariate and multivariate analyses involving data below detection limits. newly isolated enterococci faecalis bacterial strains aim06 (dsm100702) and sr14 (dsm100701) demonstrated 100% specificity and 90% sensitivity to human sewage without detecting 68 animal manure pooled samples of cats, chickens, cows, dogs, ducks, pigs, and pigeons. aim06 and sr14 bacter ... | 2017 | 27983829 |
| murine experimental root canal infection: cytokine expression in response to f. nucleatum and e. faecalis. | the aim of this study was to evaluate the gene expression of proinflammatory (rankl, tnf-a and ifn-g) and regulatory (tgf-b and il-10) cytokines as reaction to experimental infection by mono or bi-association of fusobacterium nucleatum (atcc 10953) and enterococcus faecalis (atcc 19433). f. nucleatum and e. faecalis, either in mono- or bi-association were inoculated into the root canal system (rcs) of balb/c mice. animals were sacrificed at 10 and 20 days after infection and periapical tissues s ... | 2016 | 27982237 |
| effect of silver nanoparticles on physicochemical and antibacterial properties of calcium silicate cements. | mineral trioxide aggregate (mta) and portland cement (pc) are calcium silicate cements. they have similar physicochemical, mechanical and biological properties. the addition of zirconium oxide (zro2) to pc provides radiopacity. silver nanoparticles (agnps) may improve some properties of cements. the aim of this study was to evaluate the effect of agnps on physicochemical/mechanical properties and antibacterial activity of white mta (wmta) and pc associated with zro2. the following materials were ... | 2016 | 27982226 |
| draft genome sequence of enterococcus faecalis strain ucd-pd3. | here, we present the draft genome sequence of enterococcus faecalis strain ucd-pd3. the assembly contains 2,861,314 bp in 73 contigs. this strain was isolated from a feral domestic cat (felis catus) anal sac secretion sample, as part of a project on isolating and characterizing the microbes present in feline anal sacs. | 2016 | 27979940 |
| antimicrobial resistance in enterococcus strains isolated from healthy domestic dogs. | enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. vancomycin and ampicillin minimum inhibitory concentrations (mics) and high-level aminoglycoside resistance (hlar) tests were also performed. isolates showed resist ... | 2016 | 27976593 |
| apical extrusion of intracanal bacteria following use of two engine-driven instrumentation techniques: an in vitro study. | the aim of the present study was to compare in vitro the amount of debris extruded apically from extracted teeth, using twisted files and protaper rotary during two different instrumentation systems. | 2016 | 27965505 |
| phage-inducible islands in the gram-positive cocci. | the sapis are a cohesive subfamily of extremely common phage-inducible chromosomal islands (picis) that reside quiescently at specific att sites in the staphylococcal chromosome and are induced by helper phages to excise and replicate. they are usually packaged in small capsids composed of phage virion proteins, giving rise to very high transfer frequencies, which they enhance by interfering with helper phage reproduction. as the sapis represent a highly successful biological strategy, with many ... | 2017 | 27959343 |
| in vitro evaluation of the sealing ability of three newly developed root canal sealers: a bacterial microleakage study. | the purpose of this study was to compare the sealing ability of mta fillapex, apatite root canal sealer and ah26 sealers. | 2016 | 27957271 |
| antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of enterococcus faecalis at different stages of development. | persistent infection of the root canal due to the presence of resistance bacterial species, such as enterococcus faecalis, has always been one of the most important reasons for endodontic treatment failure. this study investigated the antimicrobial efficacy of 1%, 2.5 % and 5% sodium hypochlorite in eliminating e. faecalis biofilms at different stages of development. | 2016 | 27957257 |
| the sfani restriction-modification system from enterococcus faecalis neb215 is located on a putative mobile genetic element. | | 2016 | 27941009 |
| effect of the association of nonsteroidal anti-inflammatory and antibiotic drugs on antibiofilm activity and ph of calcium hydroxide pastes. | the objective of the present study was to evaluate the in vitro antibiofilm activity and ph of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (nsaids). | 2017 | 27939738 |
| reversible-switch mechanism of the sam-iii riboswitch. | riboswitches are self-regulatory elements located at the 5' untranslated region of certain mrnas. the enterococcus faecalis sam-iii (smk) riboswitch regulates downstream gene expression through conformational change by sensing s-adenosylmethionine (sam) at the translation level. using the recently developed systematic helix-based computational method, we studied the co-transcriptional folding behavior of the smk riboswitch and its shortened construct lacking the first six nucleotides. we find th ... | 2016 | 27934232 |
| assessment of virulence potential of uncharacterized enterococcus faecalis strains using pan genomic approach - identification of pathogen-specific and habitat-specific genes. | enterococcus faecalis, a leading nosocomial pathogen and yet a prominent member of gut microbiome, lacks clear demarcation between pathogenic and non-pathogenic strains at genome level. here we present the comparative genome analysis of 36 e. faecalis strains with different pathogenic features and from different body-habitats. this study begins by addressing the genome dynamics, which shows that the pan-genome of e. faecalis is still open, though the core genome is nearly saturated. we identifie ... | 2016 | 27924951 |
| genome sequence of a commensal bacterium, enterococcus faecalis cba7120, isolated from a korean fecal sample. | enterococcus faecalis, the type strain of the genus enterococcus, is not only a commensal bacterium in the gastrointestinal tract in vertebrates and invertebrates, but also causes serious disease as an opportunistic pathogen. to date, genome sequences have been published for over four hundred e. faecalis strains; however, pathogenicity of these microbes remains complicated. to increase our knowledge of e. faecalis virulence factors, we isolated strain cba7120 from the feces of an 81-year-old fem ... | 2016 | 27924153 |
| presence and resistance of streptococcus agalactiae in vaginal specimens of pregnant and adult non-pregnant women and association with other aerobic bacteria. | aim to determine the prevalence rate and resistance profile of streptococcus agalactiae (s. agalactiae) in vaginal swabs of pregnant and adult non-pregnant women in the tuzla region, bosnia and herzegovina (b&h), as well as its association with other aerobic bacteria. methods this prospective study included 200 women, 100 pregnant and 100 adult non-pregnant. the research was conducted at the institute of microbiology, university clinical center tuzla from october to december 2015. standard aerob ... | 2017 | 27917849 |
| relationship between enterococcus faecalis infective endocarditis and colorectal neoplasm: preliminary results from a cohort of 154 patients. | the association between streptococcus bovis group infective endocarditis and colorectal neoplasm (crn) is well-known. however, no studies have assessed the association between enterococcus faecalis infective endocarditis (efie) and crn. we aimed to determine whether the prevalence of crn is higher in patients with efie and an unclear source of infection than in patients with efie and a known source of infection or in the general population. | 2017 | 27916708 |
| therapeutic effects of gold nanoparticles synthesized using musa paradisiaca peel extract against multiple antibiotic resistant enterococcus faecalis biofilms and human lung cancer cells (a549). | botanical-mediated synthesis of nanomaterials is currently emerging as a cheap and eco-friendly nanotechnology, since it does not involve the use of toxic chemicals. in the present study, we focused on the synthesis of gold nanoparticles using the aqueous peel extract of musa paradisiaca (mppe-aunps) following a facile and cheap fabrication process. the green synthesized mppe-aunps were bio-physically characterized by uv-vis spectroscopy, ftir, xrd, tem, zeta potential analysis and edx. mppe-aun ... | 2017 | 27916691 |
| antibody guided molecular imaging of infective endocarditis. | in this protocol, we describe the application of using a high affinity monoclonal antibody generated against the major pilin protein component of the pilin structure of enterococcus faecalis as a pet imaging agent for enterococcal endocarditis detection. the anti-pilin -mab 64cu conjugate was able to specifically label enterococcal endocarditis vegetation in vivo in a rodent endocarditis model. by targeting pili, a covalently linked surface antigen extending from the bacterial surface, we provid ... | 2017 | 27914083 |
| microbiological evaluation of the extension wire and percutaneous epidural lead anchor site following a "2-stage cut-down" spinal cord stimulator procedure. | there are concerns regarding bacterial colonization of the temporary extension leads and subsequent infection risk using the 2-stage cut-down approach in spinal cord stimulation (scs). we sought to quantify the extent of bacterial colonization of the temporary extension wire and percutaneous epidural lead anchor site. | 2016 | 27910210 |
| biosynthesis of selenium nanoparticles using enterococcus faecalis and evaluation of their antibacterial activities. | microorganisms are capable of synthesizing metal nanoparticles, and specifically enterococcus faecalis bacteria were tested for its ability to synthesize selenium nanoparticles (se-nps) from sodium selenite. the biosynthesized se-nps were spherical in shape with the size range of 29-195nm. also, the tem microscopy showed the accumulation of nano-structures as extracellular deposits. the ability of the bacteria to tolerate high levels of toxic selenite was studied by changing with different conce ... | 2017 | 27908405 |
| combination effect of cefuroxime and levofloxacin against bacteria isolated from the healthy conjunctival sac and endophthalmitis cases using a fractional inhibitory concentration index. | intracameral cefuroxime (cxm) and antibiotic eye drops such as fluoroquinolone are widely used for preventing endophthalmitis after cataract surgery. however, few reports are available regarding their interactions. this study was conducted to examine the in vitro interaction of cxm and levofloxacin (lvfx) against bacterial isolates from the healthy conjunctival sac before cataract surgery and from endophthalmitis cases. | 2017 | 27906556 |
| the pathogenic potential of proteus mirabilis is enhanced by other uropathogens during polymicrobial urinary tract infection. | urinary catheter use is prevalent in health care settings, and polymicrobial colonization by urease-positive organisms, such as proteus mirabilis and providencia stuartii, commonly occurs with long-term catheterization. we previously demonstrated that coinfection with p. mirabilis and p. stuartii increased overall urease activity in vitro and disease severity in a model of urinary tract infection (uti). in this study, we expanded these findings to a murine model of catheter-associated uti (cauti ... | 2017 | 27895127 |
| comparison of antibacterial efficacy of turmeric extract, morinda citrifolia and 3% sodium hypochlorite on enterococcus faecalis: an in-vitro study. | sodium hypochlorite (naocl), the most commonly used irrigant, has many potential properties like its unique ability to dissolve pulp tissue, excellent antimicrobial activity, but has a cytotoxic effect when injected into periapical tissues. it is also known to produce allergic reactions, foul smell and taste, and potential for corrosion. facultative organisms such as enterococcus faecalis and aerobes like staphylococcus aureus are considered to be the most resistant species and one of the possib ... | 2016 | 27891459 |
| comparison of the most common isolates of postoperative endophthalmitis in south korea; enterococcus species vs coagulase-negative staphylococci. | to compare the related factors or manifestations of the two most common isolates of post-operative endophthalmitis, which were enterococcus spp. and coagulase-negative staphylococci (cns) in south korea. | 2016 | 27887598 |
| bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against enterococcus faecalis. | the aim of the present study was to assess the antimicrobial activity of plasma jet with helium (he) flowing through 3% hydrogen peroxide in root canals infected with enterococcus faecalis. a total of 42 single-rooted anterior teeth were prepared, sterilized, inoculated with an e. faecalis suspension and incubated for 7 days. next, the teeth were randomly divided into six experimental groups (including groups treated by plasma jet with or without he for different time durations) and one control ... | 2016 | 27882119 |
| treatment of oral biofilms by a d-enantiomeric peptide. | almost all dental diseases are caused by biofilms that consist of multispecies communities. djk-5, which is a short d-enantiomeric, protease-resistant peptide with broad-spectrum anti-biofilm activity, was tested for its effect on oral multispecies biofilms. peptide djk-5 at 10 μg/ml effectively prevented the growth of these microbes in culture media in a time-dependent manner. in addition to the prevention of growth, peptide djk-5 completely killed both streptococcus mutans and enterococcus fae ... | 2016 | 27880799 |
| randomized clinical trial of preoperative skin antisepsis with chlorhexidine gluconate or povidone-iodine. | skin antiseptic agents are used to prevent surgical-site infection (ssi); few trials have reported the superiority of any specific agent in clean-contaminated abdominal surgery. this rct was designed to compare the effectiveness of chlorhexidine gluconate and povidone-iodine. | 2017 | 27879993 |
| identification of chemical compounds that inhibit protein synthesis in pseudomonas aeruginosa. | four inhibitory compounds were identified using a poly-uridylic acid (polyu) mrna-directed aminoacylation/translation (a/t) protein synthesis system composed of phenylalanyl-trna synthetases (phers), ribosomes, and ribosomal factors from pseudomonas aeruginosa in an in vitro screen of a synthetic compound library. the compounds were specific for inhibition of bacterial protein synthesis. in enzymatic assays, the compounds inhibited protein synthesis with ic50 values ranging from 20 to 60 μm. min ... | 2016 | 27872201 |
| synthesis of peptidoglycan fragments from enterococcus faecalis with fmoc-strategy for glycan elongation. | peptidoglycan (pgn) is an essential structural component of the bacterial cell wall conferring cell shape, which can be recognized by host-recognition proteins and receptors as well as bacterial surface proteins. in this work, the pgn partial structures from enterococcus faecalis that contain a tetrasaccharide and an octasaccharide with a unique heptapeptide were synthesized via an fmoc-strategy for elongation of the glycan chains. namely, a 4'-o-fmoc-protected disaccharide was utilized as the k ... | 2017 | 27868361 |
| intrathecal/intraventricular linezolid in multidrug-resistant enterococcus faecalis ventriculitis. | background the use of intrathecal antibiotic therapy for the treatment of ventriculitis and/or meningitis has demonstrated efficacy especially when sterilization of the cerebrospinal fluid is not possible with intravenous antibiotics alone. case description we describe the successful treatment of enterococcus faecalis ventriculitis utilizing intrathecal linezolid in a 32-year-old female patient with severe allergy to vancomycin, prohibitive bacterial susceptibilities, and failure of previous att ... | 2016 | 27867829 |
| intratubular disinfection with tri-antibiotic and calcium hydroxide pastes. | the aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes. | 2017 | 27866468 |
| effectiveness of final decontamination protocols against enterococcus faecalis and its influence on bond strength of filling material to root canal dentin. | the aim of this study was to evaluate the effectiveness of final decontamination protocols against enterococcus faecalis and their influence on bond strength of filling material to root canal dentin. | 2017 | 27866000 |
| triterpene glycosides from the aerial parts of gouania longipetala. | six previously undescribed triterpenoid saponins, gouaniaside i-vi, were isolated from the aerial parts of gouania longipetala hemsl. (rhamnaceae), in addition to four known triterpenes. the structure elucidation of these compounds was based on analyses of spectroscopic data including 1d- and 2d-nmr and hr-esi-ms techniques. the inhibitory activity of isolated compounds against promyelocytic leukemia hl60 and human erythromyeloblastoid leukemia k562 cell lines was evaluated and jujuboside i exhi ... | 2017 | 27865442 |
| co-evolutionary dynamics between a defensive microbe and a pathogen driven by fluctuating selection. | microbes that protect their hosts from pathogenic infection are widespread components of the microbiota of both plants and animals. it has been found that interactions between 'defensive' microbes and pathogens can be genotype-specific and even underlie the variation in host resistance to pathogenic infection. these observations suggest a dynamic co-evolutionary association between pathogens and defensive microbes, but direct evidence of co-evolution is lacking. we tested the hypothesis that def ... | 2016 | 27862515 |
| comparative assessment of antimicrobial efficacy of different hand sanitizers: an in vitro study. | to evaluate the antimicrobial efficacy of four different hand sanitizers against staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, and enterococcus faecalis as well as to assess and compare the antimicrobial effectiveness among four different hand sanitizers. | 2016 | 27857768 |
| most probable number - loop mediated isothermal amplification (mpn-lamp) for quantifying waterborne pathogens in <25min. | we are reporting a most probable number approach integrated to loop mediated isothermal technique (mpn-lamp) focusing on gram-negative escherichia coli and gram-positive enterococcus faecalis bacterial cells without nucleic acids extraction. lamp assays for uida from e. coli and gele from e. faecalis were successfully performed directly on cells up to single digit concentration using a commercial real time pcr instrument. threshold time values of lamp assays of bacterial cells, heat treated bact ... | 2017 | 27856278 |
| comparison of antibacterial effects of 810 and 980- nanometer diode lasers on enterococcus faecalis in the root canal system -an in vitro study. | background and aim: use of laser technology in endodontics has greatly increased in the recent years due to the introduction of new wavelengths and methods and optimal antimicrobial and smear layer removal properties of lasers. this in vitro study aimed to compare the antibacterial effects of diode lasers of 810 nm and 980 nm wavelength on enterococcus faecalis (e. faecalis) biofilm in the root canal system. materials and methods: fifty single-canal human anterior teeth were cleaned, shaped, ste ... | 2016 | 27853346 |
| furostanol saponins from the bulbs of welsh onion, allium fistulosum l. | an extensive phytochemical analysis of the polar extracts from bulbs of welsh onion allium fistulosum l. led to the isolation of nine saponins, four of them, named fistulosaponins g (1a/1b), h (2), i (3a/3b), and j (4), have never been reported previously. fistulosaponins g and i were isolated as a couple of isomers in equilibrium. on the basis of 2d nmr and mass spectrometry data, the structure of the novel compounds were elucidated as (25r)-26-[(β-d-glucopyranosyl)oxy]-3β,22β-dihydroxyfurost-5 ... | 2016 | 27852096 |
| microbe-mediated host defence drives the evolution of reduced pathogen virulence. | microbes that protect their hosts from pathogens are widespread in nature and are attractive disease control agents. given that pathogen adaptation to barriers against infection can drive changes in pathogen virulence, 'defensive microbes' may shape disease severity. here we show that co-evolving a microbe with host-protective properties (enterococcus faecalis) and a pathogen (staphylococcus aureus) within caenorhabditis elegans hosts drives the evolution of reduced pathogen virulence as a by-pr ... | 2016 | 27845328 |
| effects of inactivated enterococcus faecalis on the proliferation and osteogenic induction of osteoblasts. | the present study aimed to evaluate the effects of enterococcus faecalis, inactivated by the common intracanal irrigants sodium hypochlorite (naocl) and chlorhexidine (chx), on osteoblasts. e. faecalis was inactivated with 2% chx or 5.25% naocl. subsequently, the cell counting kit‑8 assay was used to examine the effects of chx‑ and naocl-inactivated e. faecalis on mc3t3‑e1 osteoblast cell proliferation. alizarin red staining was used to determine osteoblast mineralization, and osteogenic inducti ... | 2016 | 27840919 |
| crip1, a novel immune-related protein, activated by enterococcus faecalis in porcine gastrointestinal epithelial cells. | cysteine-rich intestinal protein 1 (crip1) is an important transcriptional regulation factor during the tumor development. although it was largely studied in the human or mouse, no report has provided functional evidence for it in the swine. to date, the real sequence of porcine crip1 (pocrip1) was also still unknown. in this study, clear characteristics for the pocrip1 were represented. a 552bp pocrip1 cdna was obtained from porcine brain tissue using real time reverse transcriptase pcr. the po ... | 2017 | 27836662 |
| antimicrobial influence of different root canal filling techniques in experimentally infected human root canals. | the aim of the present study was to evaluate the antimicrobial influence of different root canal filling techniques using gutta-percha and an epoxy resin-based sealer in experimentally infected root canals of extracted human teeth. | 2017 | 27834415 |
| evaluation of bd max staph sr assay for differentiating between staphylococcus aureus and coagulase-negative staphylococci and determining methicillin resistance directly from positive blood cultures. | we evaluated the performance of the bd max staphsr assay (sr assay; bd, usa) for direct detection of staphylococcus aureus and methicillin resistance not only in s. aureus but also in coagulase-negative staphylococci (cns) from positive blood cultures. | 2017 | 27834064 |
| antimicrobial photodynamic therapy using indocyanine green and near-infrared diode laser in reducing entrerococcus faecalis. | the use of antimicrobial photodynamic therapy (apdt) has been suggested as an adjuvant method to eliminate facultative bacteria during root canal disinfection. the purpose of this preliminary in vitro study was to determine whether the light-activated antimicrobial agent, indocyanine green (icg), could be used as photosensitizer and kill enterococcus faecalis strain under planktonic conditions when irradiated with near-infared (nir) diode laser emitting in 810nm wavelength. | 2017 | 27816623 |
| investigation to test potential stereolithography materials for development of an in vitro root canal model. | the aims were to compare the physico-chemical properties (zeta-potential, wettability, surface free energy) of stereolithography materials (stl) (photopolymer, accura) to dentine and to evaluate the potential of each material to develop enterococcus faecalis biofilm on their respective surfaces. eighteen samples of each test material (photopolymer, accura, dentine) were employed (total n = 54) and sectioned to 1 mm squares (5 mm x 5 mm) (n = 15) or ground into a powder to measure zeta-potential ... | 2017 | 27813213 |
| exopolysaccharide dispelled by calcium hydroxide with volatile vehicles related to bactericidal effect for root canal medication. | enterococcus faecalis is the dominant microbial species responsible for persistent apical periodontitis with ability to deeply penetrate into the dentin. exopolysaccharides (eps) contribute to the pathogenicity and antibiotic resistance of e. faecalis. our aim was to investigate the antimicrobial activity of calcium hydroxide (ch), camphorated parachlorophenol (cmcp), and chlorhexidine (chx) against e. faecalis in dentinal tubules. | 2017 | 27812619 |
| pooled analysis of single-dose oritavancin in the treatment of acute bacterial skin and skin-structure infections caused by gram-positive pathogens, including a large patient subset with methicillin-resistant staphylococcus aureus. | oritavancin is a lipoglycopeptide antibiotic with bactericidal activity against gram-positive pathogens, including methicillin-resistant staphylococcus aureus (mrsa). the phase 3 studies solo i and solo ii demonstrated comparable efficacy and safety of a single dose of oritavancin compared with 7-10 days of twice-daily vancomycin in adults with acute bacterial skin and skin-structure infections (absssis). the present analysis assessed clinical responses by pathogen at 48-72 h and at study days 1 ... | 2016 | 27665522 |