| severe psittacosis during pregnancy and suppression of antibody response with early therapy. | a cluster of 3 cases of psittacosis occurred among members of one family. the initial case was a pregnant woman who presented with extensive multilobar infiltrates and severe respiratory distress necessitating mechanical ventilation. her respiratory failure worsened during 36 h of erythromycin therapy. improvement coincided with the termination of pregnancy and the initiation of doxycycline treatment. the remaining patients developed milder illness. serologic assessment of the affected subjects ... | 1995 | 8588147 |
| [endomycoses--a problem in the diagnosis of chlamydia psittaci infections in chickens]. | two hens, which died at an age of 42 respectively 45 weeks, showed firstly lesions of endomycosis as sole cause of illness or death at necropsy and in light microscopic investigations. with peroxidase-antiperoxidase-(pap-)technique and in electron microscopic investigations, however, chlamydia psittaci were also found in very large numbers in both chickens, and occurred here particularly in lungs, liver, kidneys, spleen, myocard and ovary. the results showed, that endomycosis and chlamydial infe ... | 1995 | 8591772 |
| probable epizootic chlamydiosis in wild california (larus californicus) and ring-billed (larus delawarensis) gulls in north dakota. | during the summer of 1986, more than 400 california gulls (larus californicus) and ring-billed gulls (larvus delawarensis), primarily fledglings, died on an island in lake sakakawea near new town, north dakota (usa). mortality was attributed largely to chlamydiosis. necropsy findings in nine carcasses included splenomegaly (n = 9), hepatomegaly (n = 4), and pericarditis (n = 1). livers from three california gulls and two ring-billed gulls, and spleens from the same five birds plus a third ring-b ... | 1995 | 8592370 |
| a survey for selected viral, chlamydial, and parasitic diseases in wild dusky-headed parakeets (aratinga weddellii) and tui parakeets (brotogeris sanctithomae) in peru. | thirty-eight free-ranging dusky-headed parakeets (aratinga weddellii) and 13 tui parakeets (brotogeris sanctithomae) were caught and released in parque nacional del manu in southeastern peru from 19 july to 5 august 1993. blood and fecal samples were collected and sera were evaluated for titers to pacheco's disease herpesvirus, psittacine polyomavirus, paramyxovirus-1, and chlamydia psittaci. fecal samples were examined for evidence of ascarid or coccidial infection by fecal flotation, and blood ... | 1995 | 8592384 |
| [immunohistochemical determination of chlamydia psittaci/pecorum and c.trachomatis in the piglet gut]. | the jejunum, ileum, caecum and colon of 200 piglets were investigated immunohistochemically for the presence of chlamydia psittaci and c. trachomatis using a vitelline igy. positive samples were later labelled using a commercial c. trachomatis polyclonal antiserum. chlamydia were present in 33 (16.4%) of the animals, and 30 out of 33 were labelled by c. trachomatis polyclonal antiserum. inclusions occurred predominantly (67%) in the large intestine. the serological results (cft, elisa) did not c ... | 1995 | 8592901 |
| application of a mycoplasma group-specific pcr for monitoring decontamination of mycoplasma-infected chlamydia sp. strains. | mycoplasma contamination of biological materials remains a major problem. most contaminations are caused by the use of mycoplasma-contaminated cell lines. we adapted a mycoplasma group-specific pcr to detect mycoplasma contamination in cell lines and demonstrate its use in monitoring decontamination procedures with mycoplasma-contaminated suspensions of chlamydia spp. three different methods were investigated: the use of mycoplasma-specific antiserum in cell culture, physical separation by the c ... | 1996 | 8593037 |
| differences in the envelope proteins of chlamydia pneumoniae, chlamydia trachomatis, and chlamydia psittaci shown by two-dimensional gel electrophoresis. | analysis by two-dimensional gel electrophoresis of the n-laurylsarkosinate(sarkosyl)-insoluble envelope complexes of l-[35]s-cysteine-labeled elementary bodies of chlamydia pneumoniae strain iol-207, chlamydia trachomatis serovar lgv2, d, and f, and chlamydia psittaci strain 6bc showed differences in the molecular charges of chlamydial outer membrane proteins. the apparent isoelectric point (pi) of the major outer membrane protein of c. pneumoniae strain iol-207 was 6.4, whereas the pi of the ma ... | 1996 | 8599533 |
| epizootic bovine abortion in a dairy herd: characterization of a chlamydia psittaci isolate and antibody response. | a chlamydial agent was recovered from the placental cotyledons of an aborting cow from a 100-cow dairy herd in cumbria. immunoblotting analysis of purified elementary bodies of the isolate revealed a reactivity pattern typical of serotype i chlamydia psittaci strains. nucleotide sequencing of the major outer membrane protein (momp) gene further confirmed the isolate, ba1, as a serotype i strain. the sequence was identical to that of the type strain of ovine enzootic abortion, b577. in both the a ... | 1995 | 8605581 |
| pregnancy failure following vaginal infection of sheep with chlamydia psittaci prior to breeding. | enzootic abortion in sheep, caused by chlamydia psittaci, has been associated with pregnancy failure in most sheep-producing countries. late-term abortions or the birth of weak low-birth-weight lambs occurred following primary c. psittaci infection in pregnant ewes. however, the mode by which c. psittaci can be transmitted among sheep has not been established. the present study was designed to determine whether the vaginal tracts of nonpregnant ewes were susceptible to c. psittaci infection and ... | 1996 | 8606067 |
| ultrastructural changes in avian chlamydia psittaci serovar a-, b-, and d-infected buffalo green monkey cells. | in order to find an explanation for the observed differences in levels of pathogenicity in turkeys of chlamydia psittaci 84/55 (avian serovar a), 89/1326 (avian serovar b), 92/1293 (avian serovar d), and the texas turkey strain (avian serovar d) (p.b. wyrick, j. choong, s.t. knight, d. goyeau, e.s. stuart, and a.b. macdonald, immunol. infect. dis. 4:131-141, 1994), the reproductive cycles of organisms of the four strains were studied in buffalo green monkey cells by transmission electron microsc ... | 1996 | 8606089 |
| parasite prevalence in free-ranging farm cats, felis silvestris catus. | no animals tested were positive for feline leukemia virus antigen and chlamydia psittaci antibodies, but all were positive for antibodies to feline calicivirus (fcv), feline herpesvirus 1 (fhv1) and rotavirus. they had antibodies to feline parvovirus (96%), feline coronavirus (84% and cowpox virus (2%). antibody to feline immunodeficiency virus (fiv) was found in 53% of animals, which were less likely to be infected with haemobartonella felis, and had higher fhv antibody titres than cats without ... | 1996 | 8620914 |
| [seroprevalence of chlamydia trachomatis infection in std consultants in morocco]. | we have conducted a seroepidemiological survey of chlamydia trachomatis infection among 400 std consultants in comparison with 400 blood donors. the study was performed by using the indirect microimmunofluorescence technique with chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae as antigens. the overall seroprevalences were 60% and 46% for std consultants and blood donors respectively. the seroprevalences of chlamydia trachomatis alone were 12.5% for std consultants and 7.5% for ... | 1995 | 8640084 |
| pathology of bovine abortion and newborn calf death caused by dual infection with chlamydia psittaci and infectious bovine rhinotracheitis virus. | nine aborted fetuses and one newborn calf, diagnosed as chlamydia psittaci (c. psittaci) infection, were pathologically examined. the characteristic lesions in the liver were focal necrosis in 9 aborted fetuses and granulomatous necrotic foci in the newborn calf. moderate numbers of intranuclear inclusion bodies were found in necrotic foci of the liver, spleen, thymus, lymph nodes, adrenal gland, kidney, lung and forestomach. immunohistologically, a small number of c. psittaci antigens was demon ... | 1996 | 8645759 |
| cytotoxic-t-lymphocyte-mediated cytolysis of l cells persistently infected with chlamydia spp. | persistent chlamydial infections have been proposed as a means whereby chlamydiae evade immune resolution of infection. such a mechanism would require evasion not only of the humoral immune responses but also of cell-mediated immune responses. we hypothesized that if such a mechanism is important, persistently infected cells should not be recognized by cytotoxic t cells. persistent infections were simulated in vitro by treatment of chlamydia trachomatis- or chlamydia psittaci-infected cells with ... | 1996 | 8675291 |
| [atypical pneumonias]. | | 1996 | 8689016 |
| [a family outbreak of chlamydia psittaci infection]. | a family outbreak (3 cases) of chlamydia psittaci infection was reported. the first case, a 56-year-old man was admitted with fever and general fatigue. chest x-ray film revealed a consolidation in the right lower lung. one month before admission he had purchased 2 parakeets (chick) and one parakeet died. on learning of his history of contact with the chick, psittacosis was suspected. after administration of fixation (cf) antibody titer against chlamydia rose to 1:128 and iga titer against chlam ... | 1996 | 8690954 |
| acute transverse myelitis associated with chlamydia psittaci infection. | we report a case of transverse myelitis presenting with fever and rapidly deteriorating neurological signs associated with chlamydia psittaci infection. it resolved with no long-standing neurological consequences. only one previous report has described transverse myelitis in association with psittacosis, and this resulted in permanent neurological sequelae. | 1996 | 8708374 |
| experimental chlamydia psittaci infection of japanese quail. | japanese quail were used for the infection model of avian chlamydiosis. one-day-old japanese quail were highly susceptible to lethal infection by a chlamydia psittaci strain of budgerigar origin upon inoculation via the air sac route with 10(4.1) ffu of the organism, showing an acute and lethal course with chlamydial propagation. in contrast, 7-day-old quail developed resistance to the infection as shown by the lack of lethal effect with the same dose. the resistance of 7-day-old birds was aboli ... | 1996 | 8709861 |
| serological diagnosis of ovine enzootic abortion by comparative inclusion immunofluorescence assay, recombinant lipopolysaccharide enzyme-linked immunosorbent assay, and complement fixation test. | since the 1950s, serological diagnosis of ovine enzootic abortion (oea), caused by strains of chlamydia psittaci, has been based mainly on the complement fixation test (cft), which is neither particularly sensitive nor specific since antibodies to other chlamydial and enterobacterial pathogens may be detected. in this study. a recombinant enzyme-linked immunosorbent assay (relisa) (medac, hamburg, germany), based on a unique chlamydial genus-specific epitope of chlamydia trachomatis l2 lipopolys ... | 1996 | 8735108 |
| molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdta encoding 3-deoxy-alpha-d-manno-octulosonic acid transferase of chlamydia pneumoniae strain tw-183. | the gene kdta of chlamydia pneumoniae strain tw-183, encoding the enzyme 3-deoxy-alpha-d-manno-octulosonic acid (kdo) transferase of lipopolysaccharide biosynthesis, was cloned and sequenced. a single open reading frame of 1314 bp was identified, the deduced amino acid sequence of which revealed 69% similarity and 43% identity with kdta of chlamydia trachomatis and chlamydia psittaci. the gene was expressed in the gram-positive host corynebacterium glutamicum and the primary gene product was cha ... | 1995 | 8748024 |
| chlamydia psittaci in turkeys: pathogenesis of infections in avian serovars a, b and d. | at 7 days of age, 4 groups, each of twenty specific pathogen free turkeys kept in isolation units were inoculated by aerosol with the texas turkey strain (avian chlamydia psittaci serovar d), strain 92/1293 (avian chlamydia psittaci serovar d), strain 84/55 (avian chlamydia psittaci serovar a) or strain 89/1326 (avian chlamydia psittaci serovar b). a fifth group of 4 specific pathogen free turkeys were sham inoculated controls. at daily intervals for 10 days and then twice weekly up to 34 days p ... | 1995 | 8748540 |
| diagnosis of ornithosis by cell culture and polymerase chain reaction in a patient with chronic pneumonia. | we report the case of a woman who had pneumonia due to chlamydia psittaci. a chlamydia species was determined to be the causative agent of the pneumonia because it was isolated from bronchoalveolar lavage fluid, because it could be detected in lung biopsy specimens by the direct immunofluorescence technique, and because chlamydia-specific antibodies could be detected by elisa and microimmunofluorescence. the infectious agent could not be identified at the species level with use of serological te ... | 1995 | 8749643 |
| the late chlamydial inclusion membrane is not derived from the endocytic pathway and is relatively deficient in host proteins. | chlamydiae are obligate intracellular parasites which multiply within infected cells in a membrane-bound structure termed an inclusion. newly internalized bacteria are surrounded by host plasma membrane; however, the source of membrane for the expansion of the inclusion is unknown. to determine if the membrane for the mature inclusion was derived by fusion with cellular organelles, we stained infected cells with fluorescent or electron-dense markers specific for organelles and examined inclusion ... | 1996 | 8751921 |
| murine granulated metrial gland cells are susceptible to chlamydia psittaci infection in vivo. | granulated metrial gland (gmg) cells are the most numerous lymphoid cells in the uteroplacental unit in rodent pregnancy. in an experimental murine model of abortion-causing infection, we have studied the responses of gmg cells to chlamydia psittaci. chlamydial inclusions have been found within gmg cells, both in apparently healthy cells and in cells with degenerative changes. establishing the existence of gmg cells infected by c. psittaci opens a new and interesting chapter in the study of thes ... | 1996 | 8751945 |
| pathogenesis of chlamydia psittaci infection in sheep: detection of the organism in a serial study of the lymph node. | sixteen sheep were inoculated subcutaneously over the left prefemoral lymph node with an abortifacient strain of chlamydia psittaci. groups of four animals were killed after 3, 6, 12 and 18 days. four of eight sheep which received a control inoculum were killed on day 6 and four on day 18. the left and right prefemoral lymph nodes were removed and weighed and portions taken from each for examination by the polymerase chain reaction (pcr), by culture, and by histopathological and immunohistochemi ... | 1996 | 8762580 |
| detection of chlamydia psittaci in enteric subclinical infections in adult sheep, through cell culture isolation. | ovine chlamydiasis is a well known disease in countries with a good practice of breeding sheep herds, not so in mexico. aiming to determine chlamydia psittaci presence in sheep herds in mexico, we sampled 10 different farmlands in 5 geographical zones on the highlands, gathering a total of 267 viable samples from adult ewes in spg (92 of them were obtained in a second sampling carried out in five randomly chosen farms). samples were treated and inoculated on l-929 cells grown in iscove's supplem ... | 1996 | 8783901 |
| comparison of procedures to detect chlamydia psittaci antibodies in cockatiels (nymphicus hollandicus). | diagnostic serologic procedures for psittacine chlamydiosis were evaluated using experimentally inoculated cockatiels (nymphicus hollandicus). chlamydia psittaci was recovered from subjects on day 16 postinoculation. seroconversion was confirmed by the direct complement fixation (dcf) test. the blocking enzyme-linked immunosorbent assay was demonstrated to be the most sensitive of three serodiagnostic procedures evaluated from inoculation until day 24 postinfection, with 92% sensitivity and 73% ... | 1996 | 8790873 |
| domiciliary outbreak of psittacosis in dogs: potential for zoonotic infection. | | 1996 | 8807786 |
| identification of a multigene family coding for the 90 kda proteins of the ovine abortion subtype of chlamydia psittaci. | while attempting to identify genes and their corresponding antigens that could be used to improve the current methods of diagnosing chlamydia psittaci infection which causes enzootic abortion in ewes, two candidate clones were isolated from a lambda gt 11 genomic dna expression library of ovine abortion subtype (strain s26/3) c. psittaci. these clones contained fragments of a gene coding for a group of three chlamydial proteins of approximately 90 kda which appeared as major immunogens by immuno ... | 1996 | 8810511 |
| differentiation of chlamydia spp. by sequence determination and restriction endonuclease cleavage of rnase p rna genes. | the amplification of dna from chlamydia trachomatis by pcr with degenerated primers yielded a 345-bp fragment of the putative rnase p rna gene. from the deduced dna sequence of this gene in c. trachomatis, a modified primer pair was designed. the primer pair was subsequently used to obtain the corresponding gene products from chlamydia pneumoniae and chlamydia psittaci. sequence comparisons revealed similarities of 76.6% between c. trachomatis and c. pneumoniae, 79.5% between c. trachomatis and ... | 1996 | 8818877 |
| [an adult case of pneumonia due to mycoplasma pneumoniae and chlamydia psittaci]. | a 18-year-old male was admitted to another hospital complaining of his chest x-ray. after transfer to our hospital, increased serum antibody titers to simultaneous m. pneumoniae and c. psittaci were noted. these antibody titers decreased after about four months. positive results for m. pneumoniae was obtained by polymerase chain reaction in the right pleural effusion. based on these findings, this case was diagnosed as m. pneumoniae and c. psittaci pneumonia. a transbronchial lung biopsy and a b ... | 1996 | 8822057 |
| two cases of lower respiratory tract infection due to chlamydia pneumonia in singapore. | chlamydia pneumoniae, previously known as chlamydia psittaci strain twar, causes both upper and lower respiratory tract infection. we report the first two cases of culture-positive chlamydia pneumoniae lower respiratory infection in singapore. both patients had underlying fibrosing alveolitis and presented with a history of prolonged productive cough and fever. chlamydia pneumoniae was isolated from the bronchoalveolar lavage fluid in the absence of other pathogens. the patients responded clinic ... | 1995 | 8838997 |
| development of a simplified polymerase chain reaction-enzyme immunoassay for the detection of chlamydia pneumoniae. | the 16s rrna genes of two chlamydia pneumoniae and two c. psittaci strains of different serovars were sequenced then compared to previously reported chlamydia 16s rrna gene sequences. chlamydia pneumoniae-specific regions were identified and specific primers for nested pcr were synthesized. nested pcr reactions were performed, in a single tube, by varying the annealing temperature of the amplification cycles. the initial thermal cycles were selected to allow annealing and extension of only the o ... | 1996 | 8849645 |
| psittacosis associated with bilateral 4th cranial nerve palsies. | we report a 55-year-old man who presented with a moderately severe community acquired pneumonia due to chlamydia psittaci, probably acquired from budgerigars. one of his presenting symptoms was diplopia, secondary to bilateral 4th cranial nerve palsies, which persisted for some months. the literature on neurological complications of pneumonia is reviewed. | 1996 | 8852553 |
| psittacosis pneumonia: a case report and review of the literature. | a case of acute, severe pneumonia with respiratory insufficiency due to chlamydia psittaci is described. rapid improvement with tetracycline therapy in all symptoms and arterial blood gases is demonstrated. the diagnosis of psittacosis was secured by both cultivation and serological verification. a comprehensive review of the literature is appended. the present report attests to the need for inclusion of c. psittaci as a possible aetiologic agent causing fulminating community-acquired pneumonia. | 1996 | 8867404 |
| diversity among abortion strains of chlamydia psittaci demonstrated by inclusion morphology, polypeptide profiles and monoclonal antibodies. | twenty eight c. psittaci abortion strains had been previously classified in to 4 immunologically distinct groups on the basis of cross-protection experiments in a mouse model. to identify the molecular basis of their immunological divergence 4 representative strains were investigated by cellular, molecular and immunological techniques. an identical pattern was obtained by alul digestion of the amplified major outer membrane protein gene (momp) by the polymerase chain reaction (pcr) of the 4 stra ... | 1996 | 8870190 |
| polymerase chain reaction for the detection of chlamydia psittaci in the feces of budgerigars. | the polymerase chain reaction (pcr) targeting the ompa gene of chlamydia psittaci was evaluated for its ability to detect chlamydiae in fecal specimens of budgerigars as compared with isolation procedures using cell culture and embryonated egg inoculations. several procedures for pcr template dna preparation were compared so as to determine their detection levels for chlamydiae propagated in cell culture in the presence of fecal materials. tween-20 and proteinase k treatments followed by centrif ... | 1996 | 8871524 |
| egg transmission of chlamydia psittaci in turkeys. | | 1996 | 8890470 |
| sequence analysis of the omp2 region of chlamydia psittaci strain gpic: structural and functional implications. | the nucleotide sequence of a 3.1-kb genomic dna fragment carrying the omp3, omp2 and srp gene homologs from chlamydia psittaci strain gpic was determined. a comparative analysis of the gpic sequence with other chlamydial omp2-linked sequences reveals highly conserved omp3 and omp2 upstream sequences across species, suggesting a unified mechanism of transcription regulation. in contrast, the omp2-srp intergenic segment, which encompasses hypothetical srp transcriptional initiation sites, is relat ... | 1996 | 8918247 |
| homologs of escherichia coli recj, gltx and of a putative 'early' gene of avian chlamydia psittaci are located upstream of the 'late' omp2 locus of chlamydia psittaci strain guinea pig inclusion conjunctivitis. | the nucleotide sequence of nearly 6 kb of genomic dna located immediately upstream of the omp3-omp2 operon of chlamydia psittaci strain gpic was obtained, revealing four significant open reading frames (orfs), named orf1, orf2, orf4 and orf5. searches for homologous sequences in the genbank/embl databases have revealed that: (a) the open-ended orf1 putatively encodes an homolog of recj of escherichia coli, thought to be required for recbcd-independent and conjugational recombination, and for uv ... | 1996 | 8918248 |
| chlamydia psittaci: prevailing problems in pathogenesis. | | 1995 | 8920109 |
| studies on the early phase of the pathogenesis of ovine enzootic abortion in the non-pregnant ewe. | the initial phase of infection of non-pregnant sheep by chlamydia psittaci (ovis) was studied by inoculating naïve and previously exposed sheep by the oro-nasal and subcutaneous routes with the bs isolate of c. psittaci (ovis). naive animals exhibited a marginal rise in temperature and seroconversion was detected by enzyme-linked immunosorbent assay (elisa) as early as 9 days after inoculation. chlamydaemia was detected using culture and an antigen detection elisa. no faecal shedding of chlamydi ... | 1995 | 8920111 |
| temporal analysis of the developing chlamydia psittaci inclusion by use of fluorescence and electron microscopy. | the chlamydiae are obligate intracellular parasites that develop and multiply within a vacuole (termed an inclusion) that does not fuse with lysosomes. inclusion morphology varies dramatically among the different chlamydiae, particularly within the species chlamydia psittaci. some strains develop within a single vacuole, while the mature inclusion of other strains consists of several distinct lobes, each filled with chlamydial developmental forms. the development of this lobed structure was inve ... | 1996 | 8926099 |
| a 28 kda major immunogen of chlamydia psittaci shares identity with mip proteins of legionella spp. and chlamydia trachomatis-cloning and characterization of the c. psittaci mip-like gene. | chlamydia psittaci strain guinea-pig inclusion conjunctivitis (gpic) produces a self-limiting ocular infection of guinea-pigs, and this condition is a representative animal model of ocular chlamydial disease. convalescent guinea-pigs, which are resistant to reinfection, produce antibodies to several elementary-body proteins, including an uncharacterized antigen of 28 kda. convalescent guinea-pig sera were used to identify, from a lambda expression library, two overlapping gpic genomic clones tha ... | 1996 | 8936321 |
| localization of chronic chlamydia psittaci infection in the reproductive tract of sheep. | twelve sheep experimentally infected with chlamydia psittaci during pregnancy either aborted or gave birth to weak, low-birth-weight lambs as a result of uteroplacental infection. subsequently, these ewes excreted chlamydial antigen from their reproductive tracts during estrus. about 1 year after pregnancy failure, immunohistochemistry and polymerase chain reaction were used to examine sections of vagina, uterus, and oviduct for evidence of c. psittaci. four noninfected control ewes were similar ... | 1996 | 8940221 |
| differentiation of chlamydia psittaci and c. pecorum strains by species-specific pcr. | sequence analyses 5' ends of the 60-kda cysteine-rich outer membrane protein genes (omp2) of chlamydia psittaci and chlamydia pecorum strains indicate that these species have approximately 70% nucleotide identity. on the basis of this sequence information, pcr primers were designed to allow the specific amplification of dna extracted from c. psittaci s26/3 (abortion strain), p94/1 (pigeon strain), and c. pecorum w73 (fecal strain) in one reaction tube. by using nested reactions (with primers pcr ... | 1996 | 8940467 |
| ambulatory patients with community-acquired pneumonia: the frequency of atypical agents and clinical course. | to determine the etiology of community-acquired pneumonia in patients treated in an ambulatory setting, using serological methods, and to compare presenting symptoms, radiographic manifestations, and clinical outcomes of patients with pneumonia of "atypical" and undetermined etiology. | 1996 | 8948274 |
| specific antigens of chlamydia pecorum and their homologues in c psittaci and c trachomatis. | to analyze the antigenic cross reactivity of various proteins of strains of chlamydia pecorum, c psitaci, and c trachomatis. samples and procedures: strains fc-stra and lw-613 of c pecorum, strains b577, fitz-9, and 68c of c psitaci, and strain lgv-2 of c trachomatis were studied. strains of c pecorum were propagated in georgia bovine kidney cells, and other chlamydial strains were propagated in l cells or georgia bovine kidney cells. partially purified chlamydial elementary bodies propagated in ... | 1996 | 8950425 |
| serological study of responses to selected pathogens causing respiratory tract infection in the institutionalized elderly. | in a prospective 2-year study, serological responses to selected pathogens were analyzed in 224 episodes of fever attributable to respiratory tract infection (51.8%) or of unknown source (48.2%) in 131 residents of two long-term-care facilities. a serological response was identified in 45 episodes (20.1%): chlamydia pneumoniae (14 episodes), haemophilus influenzae type b (1), influenza virus type a (14), respiratory syncytial virus (rsv;2), parainfluenza virus type 3 (7), c. pneumoniae and h. in ... | 1996 | 8953065 |
| comparison of pharyngeal, fecal, and cloacal samples for the isolation of chlamydia psittaci from experimentally infected cockatiels and turkeys. | direct comparisons were made of chlamydial isolation rates from pharyngeal swabs, fecal samples, and cloacal swabs from cockatiels and pharyngeal and cloacal swabs from turkeys experimentally infected with chlamydia psittaci. during pathogenesis studies, 133 paired specimens were collected from cockatiels and 118 paired specimens were collected from turkeys. of the 51 cockatiel chlamydial infections detected, 80.4% were positive by the pharyngeal swab sample, 45.1% were positive by the fecal swa ... | 1996 | 8953529 |
| studies on the safety of chlamydia psittaci vaccination in cats. | | 1995 | 8966973 |
| chlamydiae as pathogens: new species and new issues. | the recognition of genital chlamydial infection as an important public health problem was made first by the recognition of its role in acute clinical syndromes, as well as in serious reproductive and ocular complications, and secondly by our awareness of its prevalence when diagnostic tests became widely accessible. the recent availability of effective single dose oral antimicrobial therapy and sensitive molecular amplification tests that allow the use of noninvasive specimens for diagnosis and ... | 1996 | 8969247 |
| [severe course of ornithosis and its intensive care and diagnostic problems--a case report]. | ornithosis is still a life-threatening illness unless diagnosed early and adequately treated with antibiotics. diagnosis, however, is difficult because often no direct contact with birds is obvious. we report on the course of a severe case of ornithosis in a 45-year-old man who had no previous health problems. infection with chlamydia psittaci probably occurred by inhaling of contaminated dust produced by flocks of wild pigeons. a special feature of the case is that commonly used serological tes ... | 1996 | 8974405 |
| [pulmonary chlamydia infections in children]. | in the last decades chlamydial infection of the respiratory tract have been described in children. chlamydia trachomatis, the most common identifiable cause of neonatal infections conjunctivitis is also a recognised cause of pneumonia in newborns and is responsible for about one third of sexually transmitted disease. chlamydia psittaci is mainly pathogenic for birds and occasionally for human. more recently chlamydia pneumoniae has been found associated with pulmonary abnormalities, nasopharynx ... | 1996 | 8978164 |
| seroepidemiology of feline chlamydiosis by microimmunofluorescence assay with multiple strains as antigens. | the prevalence of anti-chlamydia antibodies was examined in 232 cat sera collected in 1985 and from 1993 to 1995 from laboratories and veterinary hospitals located in 11 prefectures of japan. the antibodies were determined by an indirect microimmunofluorescence test using six strains of feline chlamydia: one strain each of avian- and guinea pig-derived c. psittaci and one strain each of c.pecorum, c.pneumoniae and c.trachomatis. positive rates of igg antibodies to chlamydiae were 34.4% in 1985 a ... | 1996 | 8981349 |
| [a case of psittacosis with psychiatric symptoms, abnormal eeg, and abnormal spect]. | a 40-year-old woman was admitted to our hospital with disorientation, delusional idea, and sensory disturbance of lower face after having a fever and cough for 5 days. a chest x-ray examination revealed an infiltration in the left lower lobe field. psittacosis was diagnosed because the serum antibody titer against chlamydia psittaci was elevated. electroencephalogram showed right temporal lobe spikes. spect showed decreased accumulation of 99mtc-hmpao in the right frontal and temporal lobe. comp ... | 1996 | 8990481 |
| interferon-gamma mediates long-term persistent chlamydia psittaci infection in vitro. | the long-term anti-chlamydial effects of recombinant ovine interferon gamma (rovifn-gamma) were studied in ovine st-6 fibroblasts infected with the s26/3 strain of chlamydia psittaci. chlamydial multiplication was assessed by enzyme-linked immunosorbent assay analysis of supernate lipopolysaccharide, titration of inclusion-forming units in culture supernates, and enumeration of inclusion bodies in cultured cells at 7-day intervals. concentrations of 250 and 1000 u/ml of rovifn-gamma resulted in ... | 1996 | 9004079 |
| virulence of feline chlamydia psittaci in mice is not a function of the major outer membrane protein (momp). | the virulent strain of feline chlamydia psittaci, the cello strain, produces conjunctivitis and upper respiratory disease in cats. this same strain produces a lethal disease in mice when inoculated intraperitoneally (i.p.). in this study we have shown that the baker strain of feline c. psittaci is attenuated in the mouse model system. intraperitoneal inoculation of mice with the baker strain produced no disease but did stimulate an immune response that protected the mice from subsequent produced ... | 1996 | 9008346 |
| secretion of proinflammatory cytokines by epithelial cells in response to chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis. | chlamydia species infect epithelial cells at mucosal surfaces, and are major causes of sexually transmitted diseases. infection is characterized by inflammation which is exacerbated upon reinfection, ultimately leading to tissue damage and scarring. although central for the development of disease manifestations, little is known about the mechanisms that initiate and sustain the inflammatory response to chlamydia. infection of cervical and colonic epithelial cells with chlamydia trachomatis and c ... | 1997 | 9011579 |
| elispot assay for chlamydia-specific, antibody-producing cells correlated with conventional complement fixation and microimmunofluorescence. | chlamydia antigens cross-reactive with pneumoniae (twar), psittaci, and trachomatis strains were used to evaluate the elispot assay for detecting antigen-specific, antibody-secreting cells (asc). human blood specimens from healthy and hospitalized persons were randomly collected and tested by coating the nitrocellulose membrane at the base of microtiter wells. ficoll-separated mononuclear cells from blood specimens collected in edta were incubated in the wells with iscove's growth medium in co2 ... | 1997 | 9021524 |
| [cross-reactivity between chlamydia psittaci and acinetobacter in indirect immunofluorescence assays]. | cross reactivity between chlamydia psittaci and a strain from genus acinetobacter was investigated by indirect fluorescent antibody (ifa) technique. two groups of serum samples were tested: 64 belonged to patients diagnosed as psittacosis and 64 (control group) were non reactive to chlamydia psittaci by ifa. samples were incubated on smears prepared with an acinetobacter suspension for detecting igg and igm. 100% reactivity to igg was found in 1:16 serum dilution among anti-psittacosis sera, whe ... | 1996 | 9026825 |
| heparin-mediated inhibition of chlamydia psittaci adherence to hela cells. | the adherence of human strains of chlamydia trachomatis has been recently shown to be inhibitable by heparin and heparitinase, leading to the proposal that chlamydia binding to host cells may be mediated by a glycosaminoglycan (gag)-dependent mechanism. we here describe the adherence of the guinea-pig pathogen, chlamydia psittaci gpic, to hela cells, which was measured by cytofluorometry with chlamydiae whose dna was fluorescently labelled. adherence could be inhibited by heat or trypsin pretrea ... | 1997 | 9032762 |
| cross-reactive cytotoxic t-lymphocyte-mediated lysis of chlamydia trachomatis- and chlamydia psittaci-infected cells. | cells infected with chlamydia trachomatis are lysed by cd8+ t cells in vitro. the ability of c. trachomatis-elicited spleen cells to lyse target cells infected with other chlamydial strains was determined by measuring lysis by immune spleen cells of targets infected with three strains of c. trachomatis and two strains of c. psittaci. c. trachomatis (lymphogranuloma venereum [lgv])-elicited immune murine spleen cells lysed target cells infected with other c. trachomatis serovars, although with lo ... | 1997 | 9038302 |
| diversity of feline chlamydia psittaci revealed by random amplification of polymorphic dna. | dna samples from c. psittaci including 6 strains of feline origin, 10 strains of avian origin, 1 strain of ovine origin and 1 strain of guinea pig origin were amplified each with three 10-nucleotide (nt) primers and four > 18-nt primers. amplified products were separated by polyacrylamide gel electrophoresis. eight patterns were recognized by random amplification of polymorphic dna (rapd) fingerprinting of c. psittaci: 2 patterns of feline origin, 5 patterns of avian origin and 1 pattern of guin ... | 1997 | 9050172 |
| the prevalence of chlamydia psittaci infections in belgian commercial turkey poults. | the prevalence of chlamydia psittaci infections in belgian commercial turkey poults was examined and a follow-up study of one belgian turkey flock was performed. sera were examined for the presence of anti-chlamydia antibodies by immunoblotting. cloacal and conjunctival swab smears and lung impression smears were examined for the presence of chlamydial antigen using the imagen chlamydia immunofluorescence test. anti-chlamydia antibodies were found in 90 of 100 sera collected at slaughter from tu ... | 1997 | 9050173 |
| evaluation of chlamydia psittaci subfraction and subunit preparations for their protective capacities. | a mouse toxicity potency test was used to evaluate the protective capacity of chlamydia psittaci (baker strain) subfraction and subunit preparations. the elementary body (eb), chlamydial outer membrane complex (comc), major outer membrane protein (momp), lipopolysaccharide (lps), and momp mixed together with lps were utilized as antigens in vaccine preparation. the ebs and comcs were prepared by centrifugation and detergent extraction as previously described for chlamydia trachomatis. isolation ... | 1996 | 9054123 |
| diagnosis of ovine enzootic abortion, using a competitive elisa based on monoclonal antibodies against variable segments 1 and 2 of the major outer membrane protein of chlamydia psittaci serotype 1. | to develop and evaluate a competitive elisa (celisa) specific for detection of antibodies to abortion strains of chlamydia psittaci and c pecorum that is based on monoclonal antibodies against the 2 segments. procedura: monoclonal antibodies were screened for binding to elisa plates coated with elementary bodies, and were selected on the basis of positive competition with experimentally produced sera against c psittaci and lack of competition with anti-c pecorum sera. the celisa was evaluated wi ... | 1997 | 9055966 |
| detection of chlamydia psittaci dna in avian clinical samples by polymerase chain reaction. | a polymerase chain reaction (pcr) assay was developed to detect. chlamydia psittaci dna in faeces and tissue samples from avian species. primers were designed to amplify a 264 bp product derived from part of the 5' non-translated region and part of the coding region of the ompa gene which encodes the major outer membrane protein. amplified sequences were confirmed by southern hybridization using an internal probe. the sensitivity of the combined assay was found to be between 60 to 600 fg of chla ... | 1997 | 9057259 |
| sero-prevalence of chlamydia infections in under five children. | | 1996 | 9057382 |
| a seroepidemiologic study of chlamydia pneumoniae, chlamydia trachomatis and chlamydia psittaci in different populations on the mainland of china. | to evaluate and compare the prevalence of antibodies to 3 chlamydial species in various populations on the mainland of china, the mif test was used to detect both serum igg and igm antibodies to these species in the normal population (n = 711), in poultry workers (n = 51), in female prostitutes (n = 106), in patients attending the sexually transmitted diseases clinic (n = 98), and in adult patients with pneumonia and bronchitis (n = 108). in the normal population, igg antibodies against c. pneum ... | 1996 | 9060055 |
| molecular cloning, sequence analysis and functional characterization of the gene kdsa, encoding 3-deoxy-d-manno-2-octulosonate-8-phosphate synthase of chlamydia psittaci 6bc. | the kdsa gene encoding 3-deoxy-d-manno-2-octulosonate-8-phosphate (kdo-8-p) synthase of chlamydia psittaci 6bc was cloned by complementing the temperature-sensitive kdsa mutant salmonella enterica serovar typhimurium ag701i50. the sequence analysis of a recombinant dna fragment revealed an open reading frame of 807 nucleotides which codes for a polypeptide of 269 amino acids with a high degree of similarity to known kdsa proteins. in addition, alignments of kdo-8-p synthases with bacterial and f ... | 1997 | 9063447 |
| activity of neutrophilic granulocytes in rabbits immunized with chlamydia psittaci. | the studies were performed on rabbits preimmunized with killed chlamydia psittaci antigen (czech isolate). in the blood of the animals nitrotetrazolium blue reduction test (spontaneous and stimulated) was performed, coefficient of polymorphonuclear cell metabolic activity in nbt test was calculated and myeloperoxidase activity was estimated, haematologic parameters were established and antibodies to chlamydia psittaci were searched for using complement fixation test. the animals were subjected t ... | 1995 | 9071449 |
| in vitro and in vivo antichlamydial activities of hsr-903, a new fluoroquinolone antibiotic. | the in vitro and in vivo antichlamydial activities of hsr-903 were investigated. the mics of hsr-903 for different species of chlamydia were 0.016 to 0.063 microg/ml, which were superior to those of conventional fluoroquinolones. the therapeutic effect of hsr-903 in experimental mouse chlamydia psittaci pneumonia was also excellent and almost equal to that of minocycline and superior to that of ofloxacin. these results indicate that hsr-903 may be useful in the treatment of respiratory infection ... | 1997 | 9087506 |
| possible nosocomial transmission of psittacosis. | to investigate a cluster of seven pneumonia cases among persons exposed to a pet-shop worker hospitalized with psittacosis. | 1997 | 9090543 |
| [community acquired pneumonia. reliability of the criteria for deciding ambulatory treatment]. | to determine whether criteria for not admitting community-acquired pneumonia (cap) patients diagnosed in the emergency room are appropriate, and to characterize the symptoms, etiology and course of cap. this one-year prospective, protocol study of immunocompetent cap patients diagnosed in the emergency room of our hospital enrolled patients not considered to require hospital admission according to the recommendations of the spanish society of respiratory disease (separ). medical histories, chest ... | 1997 | 9091117 |
| [significance of causes of infectious abortion in sheep flocks in northern baden-württemberg with special reference to chlamydia psittaci]. | investigations on the reasons of infectious abortion cases in sheep flocks in northern parts of baden-wuerttemberg eludicate the wide-spreading of chlamydia psittaci (c. psittaci) and its significance as the most frequent cause of abortions in sheep. another important pathogen agent causing abortions is salmonella abortus ovis (s. abortus ovis) which could be demonstrated by using a serological elisa test. a less important role than c. psittaci and s. abortus ovis plays coxiella burnetii in abor ... | 1997 | 9092464 |
| psittacosis. | pneumonia caused by chlamydia psittaci likely occurs considerably more frequently than is suggested by the mere 100 to 200 cases reported annually to public health authorities. parrots and other psittacine birds still are regarded as the major reservoir of the infectious agent and most recognized cases are associated with owning pet birds or working in a pet store. it was once thought that birds imported from abroad, often illegally, were a principal source, but many domestic breeder flocks of p ... | 1997 | 9097370 |
| phylogenetic analysis of the genus chlamydia based on 16s rrna gene sequences. | the phylogenetic relationships among chlamydia spp. were investigated by comparing 16s rrna gene sequences. in this analysis we used 14 strains of chlamydia psittaci, including seven feline isolates, two avian isolates, two human isolates, one bovine isolates, one ovine isolate, and one koala isolate; and nine strains of chlamydia trachomatis, including six human isolates, two swine isolates, and one mouse isolate. a phylogenetic analysis of the 16s rrna gene sequences of these organisms and sev ... | 1997 | 9103632 |
| the ribosomal intergenic spacer and domain i of the 23s rrna gene are phylogenetic markers for chlamydia spp. | current methods used to classify chlamydia strains, including biological, morphological, and dna hybridization techniques and major outer membrane protein (omp1) gene analysis, can be imprecise or difficult to perform. to facilitate classification, 2.8-kb partial ribosomal dna (rdna) segments from a chlamydia trachomatis strain and a chlamydia psittaci strain were amplified by pcr and sequenced. subsequently, a 1,320-bp region in this segment, including both the 16s/23s intergenic spacer (232 +/ ... | 1997 | 9103637 |
| comparison of chlamydia psittaci from cats with upper respiratory tract disease by polymerase chain reaction analysis of the ompa gene. | conjunctival swabs were taken from 168 cats with clinical signs of acute or chronic upper respiratory tract disease and tested for chlamydia psittaci by the polymerase chain reaction (pcr) to amplify the ompa gene coding region. twenty-two (13 per cent) were positive for c psittaci. the pcr products from positive samples were subjected to restriction endonuclease analysis with the restriction enzymes alu i and mse i. the fragments of dna were detected on silver-stained polyacrylamide gels and th ... | 1997 | 9106964 |
| myopericarditis due to chlamydia psittaci. the role of autoimmunity. | chlamydia psittaci may rarely involve the heart as the only manifestation of the infection. we report a case of myopericarditis caused by this agent, whose pathogenesis seemed to be autoimmune damage triggered by the infection. | 1997 | 9112307 |
| species identification of chlamydia isolates by analyzing restriction fragment length polymorphism of the 16s-23s rrna spacer region. | the genetic diversity of the 16s-23s rrna spacer region of 12 chlamydia pneumoniae isolates, 7 chlamydia trachomatis isolates (human biovars: the trachoma serovars b and c, the urogenital serovars d, e, and f, and the lymphogranuloma venereum serovar l2; and a mouse biovar), 6 chlamydia psittaci isolates (5 avian isolates and 1 feline isolate), and one chlamydia pecorum isolate was studied. the 16s-23s rrna spacer region was amplified by pcr and digested with the restriction enzymes msei, psti, ... | 1997 | 9114403 |
| use of monoclonal antibodies to facilitate identification, cloning, and purification of chlamydia trachomatis hsp10. | as a requisite for a physiological and immunological investigation, reagents were developed that facilitated the identification and purification of chlamydia trachomatis hsp10 (chsp10). monoclonal antibodies that specifically recognize chsp10 were generated with multiple-antigen peptides (maps) to promote recognition of chlamydia-specific epitopes. map2, containing amino acids 54 to 69 of the hsp10 sequence, elicited strong antibody responses after immunization of balb/c mice. monoclonal antibod ... | 1997 | 9114409 |
| comparison of polymerase chain reaction, direct immunofluorescence, cell culture and enzyme immunoassay for the detection of chlamydia psittaci in bull semen. | the polymerase chain reaction (pcr), direct immunofluorescence (dif; jmagen chlamydia, dako diagnostics, uk), cell culture (cc) and enzyme immunoassay (eia; syva micro trak) were evaluated for detection of chlamydia psittaci in bull semen. three specimens were collected from each of 47 bulls at 3-6 month intervals (134 samples). judging by the number of samples tested (n = 134), pcr showed a sensitivity of 90.9%, dif of 93.9%, cc of 72.7% and eia of 81.8%. pcr, dif, cc and eia were 100% specific ... | 1994 | 9133052 |
| experimental infections of pregnant sows with ovine chlamydia psittaci strains. | four sows were experimentally inoculated at 42 days pregnancy, with the bs isolate of chlamydia psittaci from aborted ewes. their body temperature and health were monitored until 30 days after farrowing. the sows seroconverted but their reproductive performance was not impaired. chlamydiae were identified in the afterbirths from two sows with normal litters using stained smears, "clearview" and "ideia" elisa antigen detection tests. the presence of inclusion bodies was detected in sections of th ... | 1994 | 9133063 |
| chlamydia psittaci inca is phosphorylated by the host cell and is exposed on the cytoplasmic face of the developing inclusion. | chlamydiae are obligate intracellular bacteria that replicate within a non-acidified vacuole called an inclusion. chlamydia psittaci (strain gpic) produces a 39 kda protein (inca) that is localized to the inclusion membrane. while inca is present as a single 39 kda species in purified reticulate bodies, two additional higher m(r) forms are found in c. psittaci-infected cells. this finding suggested that inca may be post-translationally modified in the host cell. here we present evidence that inc ... | 1997 | 9140978 |
| analysis of partial 16s rrna nucleotide sequences of chlamydia pecorum and c. psittaci. | partial 16s nucleotide sequences of chlamydia psittaci isolates s26/3 (abortion), p94/1 (pigeon) and chlamydia pecorum isolates w73 (enteric) and e58 (encephalomyelitis) were determined. analysis of these data indicates very high levels of interspecies sequence conservation, with c. psittaci being more closely related to c. pecorum than to c. pneumoniae or c. trachomatis. restriction enzyme analysis of nucleotide sequences indicated that bsli can be used to clearly distinguish c. psittaci and c. ... | 1997 | 9143877 |
| analytic performance and contamination control methods of a ligase chain reaction dna amplification assay for detection of chlamydia trachomatis in urogenital specimens. | a ligase chain reaction (lcr) dna amplification assay that targeted the cryptic plasmid of chlamydia trachomatis was developed to detect c. trachomatis urogenital tract infection. the objectives of this study were to determine the cutoff and analytic performance of the lcr assay and to characterize the effectiveness of its postdetection contamination control method. the assay's cutoff was determined after receiver-operator characteristic (roc) analysis of 4660 clinical data points. the assay det ... | 1996 | 9147911 |
| [avian chlamydia psittaci phyla and their pathogenic significance for turkeys]. | the present study demonstrates that chlamydia psittaci is an important cause of respiratory disease in turkeys in europe. the serotyping of isolates revealed that european turkeys frequently become infected with chlamydia psittaci serovar d strains, but also can become infected with serovar a and b strains. in turkeys, differences in pathogenicity were observed not only between strains belonging to the serovars a (strain 84/55), serovar b (strain 89/1326) and serovar d (texas turkey strain and s ... | 1996 | 9157743 |
| [occurrence of antibodies to chlamydial group antigens in the population of the czech republic]. | a set of 4,178 human blood sera was tested including 3,093 samples collected from blood donors, 624 randomly collected samples. 124 samples collected from persons possibly exposed to chlamydia infection, and 337 samples from patients of various hospitals in the districts of brno, olomouc and rumburk. all the sera were examined using the complement fixation microtest and the antigen of chlamydia psittaci for cfr (bioveta, ivanovice na hané, czr). moreover, a subset of samples collected from selec ... | 1997 | 9162449 |
| localization by immunoelectron microscopy of antigens of chlamydia psittaci suitable for diagnosis or vaccine development. | two different antigens of serotype 1 chlamydia psittaci were localized using three immunoelectron microscopy techniques: non-embedding, pre-embedding and post-embedding. the antigens had previously been described as being of potential use in diagnosis (80-90 kda protein region) and vaccine development (110 kda protein). the results show a direct relationship between the protective capacity of the antigens and their surface localization on the elementary bodies, which are the infectious form of c ... | 1997 | 9163915 |
| distribution of endosomal, lysosomal, and major histocompatability complex markers in a monocytic cell line infected with chlamydia psittaci. | the intracellular fate of chlamydia psittaci during infection of a monocytic cell line, thp1, was characterized. cytochalasin d inhibited phagocytosis of latex beads but had no effect on infection by c. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocytic pathway. early chlamydia-containing vacuoles expressed major histocompatibility complex (mhc) class i molecules, and most vacuoles fused with host cell lysosomes, since they expressed lamp-1 and h ... | 1997 | 9169786 |
| in vitro and in vivo activities of am-1155, a new fluoroquinolone, against chlamydia spp. | the in vitro and in vivo activities of am-1155, a new quinolone, against chlamydia spp. were investigated. the mic of am-1155 for 10 standard strains of different chlamydia spp. and 25 wild-type strains of chlamydia pneumoniae isolated in japan, which were morphologically different from clinical isolates from the united states, ranged from 0.063 to 0.125 microg/ml. its activity was almost the same as those of sparfloxacin and tosufloxacin and was 4 and 16 times superior to those of levofloxacin ... | 1997 | 9174194 |
| infective endocarditis due to unusual or fastidious microorganisms. | infective endocarditis due to fastidious microorganisms is commonly encountered in clinical practice. some organisms such as fungi account for up to 15% of cases of prosthetic valve infective endocarditis, whereas organisms of the hacek group (haemophilus parainfluenzae, h. aphrophilus, and h. paraphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae) cause 3% of community-acquired cases of infective endocarditis. special techniques a ... | 1997 | 9179137 |
| gestational psittacosis: case report and literature review. | in europe, chlamydia psittaci is a relatively common cause of abortion in sheep and other mammals. psittacosis in humans is typically described as a mild-to-moderate flu-like illness. if psittacosis is acquired during pregnancy, it can present as a severe, progressive, febrile illness, with headache, disseminated intravascular coagulation, abnormal liver enzyme studies, and impaired renal function. only cases with significant fetomaternal morbidity and mortality have been reported. recovery from ... | 1997 | 9195579 |
| polymerase chain reaction (pcr) detection of porcine chlamydia trachomatis and ruminant chlamydia psittaci serovar 1 dna in formalin-fixed intestinal specimens from swine. | in previous studies chlamydiae were detected immunohistologically in the gut of 66 out of 311 pigs. the aim of the present investigation was the classification of these intestinal porcine chlamydiae. for the study, dna extracted from 52 paraffin-embedded intestinal tissues was amplified in nested polymerase chain reactions (pcrs) with chlamydia omp1 genus- and species-specific primers. some of the amplification products were cloned and sequenced. in 45 cases dna could be amplified with genus-spe ... | 1997 | 9197211 |
| some characteristics of a smooth type lipopolysaccharide of chlamydia psittaci. | | 1997 | 9199717 |
| chlamydiosis in captive white-winged doves (zenaida asiatica). | chlamydia psittaci was isolated from the spleen of a moribund white-winged dove (zenaida asiatica). the isolate was serotyped as the serovar b that is commonly isolated from pigeons. a fourfold increase in the titer of antichlamydial igm activity occurred in that bird in paired serum samples tested by chlamydial elementary body agglutination (eba) and a greater than or equal to fourfold decrease of igg occurred by direct complement fixation (dcf). the increases or decreases of eba and dcf titers ... | 1997 | 9201425 |
| plasmid diversity in chlamydia. | chlamydiae exhibit low interspecies dna homology and plasmids from different chlamydial species can be readily distinguished by southern blot analysis and restriction enzyme profiling. in contrast, available plasmid sequence data from within the species chlamydia trachomatis indicate that plasmids from human isolates are highly conserved. to evaluate the nature and extent of plasmid variation, the complete nucleotide sequences were determined for novel plasmids from three diverse non-human chlam ... | 1997 | 9202459 |
| gestational psittacosis in a montana sheep rancher. | in humans, psittacosis is primarily a flulike illness following exposure to psittacine birds. in rare cases, pregnant women exposed to chlamydia psittaci can contract gestational psittacosis: atypical pneumonia, sepsis, and placental insufficiency resulting in premature birth or miscarriage. in the united states, only two cases of gestational psittacosis have been reported, both from exposure to psittacine birds. eleven other cases have been reported worldwide, mostly in the united kingdom, all ... | 1997 | 9204302 |