| [status of clonorchis sinensis infection and its influencing factors among migrant workers in baoan district, shenzhen city]. | to understand the status of clonorchis sinensis infection and its risk factors among migrant workers in baoan district of shenzhen city, so as to provide the evidence for the development of control strategies. | 2013 | 23687830 |
| use of black carp (mylopharyngodon piceus) in biological control of intermediate host snails of fish-borne zoonotic trematodes in nursery ponds in the red river delta, vietnam. | the risks of fish-borne zoonotic trematodes (fzt) to human health constitute an important problem in vietnam. the infection of humans with these trematodes, such as small liver trematodes (clonorchis sinensis and opisthorchis viverrini), intestinal trematodes (heterophyidae) and others is often thought to be linked to fish culture in areas where the habit of eating raw fish is common. juvenile fish produced in nurseries are often heavily infected with fzt and since fishes are sold to aquaculture ... | 2013 | 23680382 |
| biochemical characterization and functional analysis of fructose-1,6-bisphosphatase from clonorchis sinensis. | fructose-1,6-bisphosphatase (fbpase), a key regulatory enzyme of gluconeogenesis, plays an essential role in metabolism and development of most organisms. to the wealth of available knowledge about fbpase from clonorchis sinensis (csfbpase), in this study, the characteristics of csfbpase and its potential role in pathogenesis of clonorchiasis were investigated. the km value of csfbpase was calculated to be 41.9 um. the optimal temperature and ph of csfbpase were 37 °c and ph 7.5-8.0, respectivel ... | 2013 | 23652997 |
| fatal multi-organ clonorchis sinensis infection in dog: a case report. | we describe here a fatal case of a dog with extensive clonorchis sinensis (c. sinensis) infection. c. sinensis were detected in organs including the abdomen, bladder and heart. the infection was very heavy with a total number of 155,183 worms. these worms were in different developmental stages, but the majority of them were adult. | 2013 | 23643359 |
| molecular characterization and expression of rab7 from clonorchis sinensis and its potential role in autophagy. | accumulating evidences suggest that rab7 gtpase is important for the normal progression of autophagy. however, the role of rab7 gtpase in regulation of autophagy in clonorchis sinensis is not known. in this study, a gene encoding rab7 was isolated from c. sinensis adult cdna. recombinant csrab7 was expressed and purified from escherichia coli. csrab7 transcripts were detected in the cdna of adult worm, metacercaria, cercaria, and egg of c. sinensis, and were highly expressed in the metacercaria. ... | 2013 | 23609597 |
| expression, immunolocalization, and serological reactivity of a novel sphingomyelin phosphodiesterase-like protein, an excretory/secretory antigen from clonorchis sinensis. | clonorchiasis, caused by clonorchis sinensis infection, is a zoonotic parasitic disease of hepatobiliary system in which the proteins released by adult are major pathogenetic factors. in this study, we first characterized a putative sphingomyelin phosphodiesterase (cssmpase) a-like secretory protein, which was highly expressed in the adult worm. the full-length gene was cloned. the putative protein is of relatively low homology comparing with smpase from other species, and of rich t cell and b c ... | 2013 | 23568580 |
| genetic variation among clonorchis sinensis isolates from different hosts and geographical locations revealed by sequence analysis of mitochondrial and ribosomal dna regions. | the present study examined genetic variability among clonorchis sinensis isolates from four different geographical localities (guangzhou, nanning, jiamusi and daqing) and host species (cats, dogs, human and rabbits) in mainland china by sequence analyses of two mitochondrial dna (mtdna) genes, namely nadh dehydrogenase subunits 2, 5 (nad2 and nad5) and ribosomal internal transcribed spacer 1 (its1). a portion of the its1, nad2 (pnad2) and nad5 (pnad5) was amplified by polymerase chain reaction s ... | 2013 | 23463938 |
| identification and biochemical characterization of adenylate kinase 1 from clonorchis sinensis. | adenylate kinase 1 is responsible for the conversion of amp into adp involved in purine metabolism. in the present study, adenylate kinase 1 gene (csadk1) was isolated from an adult cdna library of clonorchis sinensis, and the recombinant protein was expressed in escherichia coli. bioinformatics analysis implied that the putative protein contained 197 amino acids, and some residues in conservative binding sites of csadk1 were substituted. the structure modeling analysis showed that csadk1 was co ... | 2013 | 23455935 |
| molecular identification, immunolocalization, and characterization of clonorchis sinensis calmodulin. | one cdna clone (cs18h09) encoding clonorchis sinensis calmodulin (cscam) was isolated from our adult cdna plasmid library. the open reading frame of cscam contains 450 bp which encodes 149 amino acids. cscam protein comprises four calcium-binding ef-hand motifs. the amino acid sequence of cscam shares very high homology with other species. quantitative rt-polymerase chain reaction (pcr) revealed that cscam mrna was constitutively transcribed in development cycle stages of the parasite, including ... | 2013 | 23417096 |
| identification and immunological characterization of thioredoxin transmembrane-related protein from clonorchis sinensis. | thioredoxin transmembrane related protein (tmx), a member of thioredoxin superfamily, is localized to the endoplasmic reticulum and possesses a thioredoxin-like domain that plays an important role as an oxidoreductase. the functions of tmx in clonorchis sinensis remain to be elucidated. in this study, we cloned and characterized a novel tmx of c. sinensis (cstmx). the cstmx cdna sequence contained a 414-nucleotide open-reading frame encoding a protein of 137 amino acids. a thioredoxin domain was ... | 2013 | 23403994 |
| the carcinogenic liver fluke, clonorchis sinensis: new assembly, reannotation and analysis of the genome and characterization of tissue transcriptomes. | clonorchis sinensis (c. sinensis), an important food-borne parasite that inhabits the intrahepatic bile duct and causes clonorchiasis, is of interest to both the public health field and the scientific research community. to learn more about the migration, parasitism and pathogenesis of c. sinensis at the molecular level, the present study developed an upgraded genomic assembly and annotation by sequencing paired-end and mate-paired libraries. we also performed transcriptome sequence analyses on ... | 2013 | 23382950 |
| [comparison between two expression ways on cysteine protease of clonorchis sinensis]. | to compare the prokaryotic expression system and eukaryotic expression system for the expression of cysteine protease of clonorchis sinensis, and the diagnostic efficiency of their objective products. | 2012 | 23236796 |
| human infections with liver and minute intestinal flukes in guangxi, china: analysis by dna sequencing, ultrasonography, and immunoaffinity chromatography. | the prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of hengxuan, fusui, and shanglin villages which were known to be endemic for liver flukes in guangxi, china in may 2010. a total of 718 people were examined for helminth eggs by the kato-katz thick smear technique, ultrasonography, immunoaffinity chromatography, and dna sequencing. the overall egg positive rate was found to be 59.6% (28.0-70.6%) that included mixed infections with liver and intestinal ... | 2012 | 23230343 |
| in vitro maintenance of clonorchis sinensis adult worms. | clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in east asia. the present study investigated survival longevity of c. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. the worms were maintained in 0.85% nacl, 1×pbs, 1×locke's solution, rpmi-1640, dmem, and imdm media, and in 1×locke's solution with different supplements. all of the worms died ... | 2012 | 23230328 |
| efficacy and safety of tribendimidine against clonorchis sinensis. | clonorchiasis is of considerable public health importance, particularly in the people's republic of china (pr china), where most of the 15 million individuals infected with clonorchis sinensis are currently concentrated. praziquantel is the drug of choice, but tribendimidine might be an alternative. | 2013 | 23223597 |
| molecular changes in opisthorchis viverrini (southeast asian liver fluke) during the transition from the juvenile to the adult stage. | the southeast asian liver fluke (opisthorchis viverrini) chronically infects and affects tens of millions of people in regions of asia, leading to chronic illness and, importantly, inducing malignant cancer (= cholangiocarcinoma). in spite of this, little is known, at the molecular level, about the parasite itself, its interplay with its hosts or the mechanisms of disease and/or carcinogenesis. | 2012 | 23209858 |
| stage-specific expression, immunolocalization of clonorchis sinensis lysophospholipase and its potential role in hepatic fibrosis. | lysophospholipase, belonging to the complex family of phospholipases, is supposed to play a vital role in virulence and pathogenesis of parasites and fungi. in the current study, the potential role of clonorchis sinensis lysophospholipase (cslysopla) in hepatic fibrosis induced by c. sinensis was explored for the first time. in the liver of the cat infected with c. sinensis, cslysopla was recognized in the lumen between adult worms and surrounding bile duct epithelia together with some inside th ... | 2013 | 23183703 |
| identification, sequence analysis and characterization of clonorchis sinensis ubiquitin. | ubiquitin is a functionally important protein expressed in eukaryotic cells usually encoded by multigenic families containing two types of genes, ubiquitin extension genes and polyubiquitin genes. one independent monomeric locus and two independent polyubiquitin loci were firstly identified from the genome of carcinogenic liver fluke, clonorchis sinensis (c. sinensis). the nucleotide and amino acid sequence of c. sinensis polyubiquitin, especially polyubiquitin with five tandem ubiquitin repeats ... | 2013 | 23149166 |
| [detection of clonorchis sinensis eggs in the ground gallbladder stones by microscopy]. | sera, feces, bile and gallbladder stones were collected from 179 patients who accepted gallbladder-preserving cholelithotomy during the period of january to june 2010 at the general surgery department in the second people's hospital of panyu district in guangzhou. rapid colloidal gold immunochromatography was used to detect igg against clonorchis sinensis. c. sinensis eggs were examined by fecal direct smear, and in bile sediments and ground gallbladder stones. the results showed that the positi ... | 2012 | 23072161 |
| a review of the control of clonorchiasis sinensis and taenia solium taeniasis/cysticercosis in china. | clonorchiasis sinensis and taenia solium taeniasis/cysticercosis are major foodborne parasitoses. clonorchiasis sinensis is actively transmitted in some areas of china, korea, russia, vietnam, etc. currently, it is estimated that more than 200 million people are at risk of infection, 15-20 million people are infected, and 1.5-2 million show symptoms or complications. in china, it is relatively heavily transmitted in zhujiang river delta, including hong kong and macao, and northeast china, where ... | 2012 | 23052782 |
| analysis of the results of two nationwide surveys on clonorchis sinensis infection in china. | to examine clonorchis sinensis infection in china and evaluate the effectiveness of efforts to prevent and control it, two nationwide surveys were undertaken in 31 provinces, autonomous regions, and municipalities (pams) during 1988-92 (the 1990 survey) and during 2001-04 (the 2003 survey). | 2012 | 22998822 |
| comparative morphology of minute intestinal fluke eggs that can occur in human stools in the republic of korea. | the egg morphology of minute intestinal flukes (mif) that can occur as human infections in the republic of korea, i.e., metagonimus yokogawai, m. miyatai, m. takahashii, heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus, stictodora fuscata, pygidiopsis summa, and gymnophalloides seoi, was studied in comparison with clonorchis sinensis. the adult worms were obtained from residents of endemic areas, and their intrauterine eggs were studied and measured using light microscopy; t ... | 2012 | 22949747 |
| the tumorigenic liver fluke opisthorchis viverrini--multiple pathways to cancer. | liver fluke infection caused by opisthorchis viverrini is a major public health problem in thailand and adjacent countries. in addition to infection-associated morbidity, infection with o. viverrini and the related clonorchis sinensis are unarguable risk factors for cholangiocarcinoma (caa, bile-duct cancer). here we review the pathogenesis of opisthorchiasis and the association between o. viverrini infection and bile-duct cancer, focusing on the molecular parallels between wound healing, chroni ... | 2012 | 22947297 |
| molecular characterization of an α-n-acetylgalactosaminidase from clonorchis sinensis. | the α-n-acetylgalactosaminidase (α-nagal) is an exoglycosidase that selectively cleaves terminal α-linked n-acetylgalactosamines from a variety of sugar chains. a complementary dna (cdna) clone encoding a novel clonorchis sinensis α-nagal (cs-α-nagal) was identified in the expressed sequence tags database of the adult c. sinensis liver fluke. the complete coding sequence was 1,308 bp long and encoded a 436-residue protein. the selected glycosidase was manually curated as α-nagal (ec 3.2.1.49) ba ... | 2012 | 22926676 |
| cholecystolithiasis is associated with clonorchis sinensis infection. | the objective of this study was to analyze gallbladder stones for direct evidence of a relationship between clonorchis sinensis infection and gallbladder stones formation. | 2012 | 22905137 |
| characterization of constituents and anthelmintic properties of hagenia abyssinica. | the dried female flowers of hagenia abyssinica (bruce) j. f. gmel. (rosaceae) are traditionally used as an anthelmintic remedy in ethiopia and formerly were incorporated into the european pharmacopoeia. one-, two- and tricyclic phloroglucinol derivatives (kosins) were suggested to be the active principles. however, polar constituents may also contribute to the activity. therefore, we investigated for the first time the polar constituents. we isolated typical rosaceae constituents such as quercet ... | 2017 | 22896828 |
| genetic diversity of nuclear its1-5.8s-its2 rdna sequence in clonorchis sinensis cobbold, 1875 (trematoda: opisthorchidae) from the russian far east. | the present study examined the molecular organisation and sequence variation in the nuclear ribosomal dna (rdna) region, including the two internal transcribed spacers (its1 and its2) and the 5.8s gene of the clonorchis sinensis from the russian far east. the relevant sequences from other parts of this species' area were downloaded from genbank. the results showed 100% identity for all investigated 5.8s-its2 rdna sequences. in contrast, two levels of intraspecific variations were revealed in the ... | 2012 | 22824337 |
| [nosoareas of clonorchiasis in the primorye territory]. | the mammalian liver parasite (clonorchis sinensis flukes) has spread in the primorye territory. the areas of the first intermediate hosts for the parasite mollusks of the genus parafossarulus have been found to recently become wider south-western predominantly due to lotus acclimatization, resulting in the expansion of a parasite habitation area. the intensity of newly formed foci of clonorchiasis is an order of magnitude greater than that of natural ones and they are a higher hazard since they ... | 2012 | 22774498 |
| identification and characterization of myophilin-like protein: a life stage and tissue-specific antigen of clonorchis sinensis. | clonorchiasis, caused by clonorchis sinensis infection, has been an important public health problem in china. more messages about biology and pathogenicity of c. sinensis will be better for development of new strategies for clonorchiasis control. in the current study, using data from the published genomic and cdna sequences for c. sinensis, we identify a gene encoding a myophilin-like protein (cs myophilin-like). the cdna sequence is 573 bp in length which encoded a protein of 190 amino acids. d ... | 2012 | 22752695 |
| molecular identification and characterization of leucine aminopeptidase 2, an excretory-secretory product of clonorchis sinensis. | aminopeptidases serve vital roles in metabolism of hormones, neurotransmission, turnover of proteins and immunological regulations. leucine aminopeptidases catalyze the hydrolysis of amino-acid residues from the n-terminus of proteins and peptides. in the present study, leucine aminopeptidase 2 (lap2) gene of clonorchis sinensis (c. sinensis) was isolated and identified from an adult cdna library of c. sinensis. recombinant cslap2 was expressed and purified in escherichia coli bl21. the open rea ... | 2012 | 22729885 |
| a novel calmodulin-like protein from the liver fluke, fasciola hepatica. | an 18.2 kda protein from the liver fluke, fasciola hepatica has been identified and characterised. the protein shows strongest sequence similarity to egg antigen proteins from schistosoma mansoni, schistosoma japonicum and clonorchis sinensis. the protein is predicted to adopt a calmodulin-like fold; it thus represents the third calmodulin-like protein to be characterised in f. hepatica and has been named fhcam3. compared to the classical calmodulin structure there are some variations. most noti ... | 2012 | 22727910 |
| development of a real-time pcr assay for the detection of clonorchis sinensis dna in gallbladder bile and stone samples from patients with cholecystolithiasis. | high prevalence of cholecystolithiasis in parts of east asia has been postulated to be associated with clonorchis sinensis infection. this study describes the development of a taqman-based real-time pcr assay for the detection of c. sinensis dna in gallbladder bile and stone samples from patients with cholecystolithiasis. primers and probe targeting the cytochrome c oxidase subunit 1 gene of mitochondrial dna proved to be highly specific for c. sinensis and did not amplify other related heteroge ... | 2012 | 22669695 |
| multiple microembolic brain infarctions in clonorchis sinensis infestation. | we report a case of multiple cerebral infarctions as the first symptom of hypereosinophilia with clonorchis sinensis infestation. the patient showed hypereosinophilia and elevated cardiac enzyme, and the egg test of c. sinensis was positive. ct scans of the chest and the abdomen revealed eosinophilic infiltration in both lung and liver. over a period of six weeks after praziquantel medication, he recovered. c. sinensis infestation which is endemic in east asian countries should be considered as ... | 2012 | 22656183 |
| trematode infections: liver and lung flukes. | food-borne trematodiases are an emerging public health problem in southeast asia and latin america and of growing importance for travel clinics in europe and north america. the disease is caused by chronic infections with liver, lung, and intestinal flukes. this article focuses on the most important liver and lung flukes that parasitize man, namely clonorchis sinensis, fasciola gigantica, fasciola hepatica, opisthorchis felineus, opisthorchis viverrini, and paragonimus spp. the article describes ... | 2012 | 22632646 |
| [effect of community intervention model of clonorchiasis in zhaoyuan county]. | after the community interventions of clonorchiasis control in zhaoyuan county, the infection rate of clonorchis sinensis of residents decreased from 67.43% to 7.12%, and the awareness rates of healthy knowledge and healthy behavior increased by 124.55% and 156.03%, respectively. the control effect is significant. | 2012 | 22590886 |
| predominance of il-10 and tgf-β production from the mouse macrophage cell line, raw264.7, in response to crude antigens from clonorchis sinensis. | parasitic helminths are well-known to have the ability to modulate host immune responses. in this study, we investigated the fundamental immunoregulatory mechanism of the liver fluke clonorchis sinensis (c. sinensis) using a murine macrophage raw 264.7 (raw) cell line and mouse bone marrow derived macrophages (bmdms). we found that c. sinensis crude antigen (ca) is able to differentiate macrophage raw cells into dendritic-like cells that can be detected by morphological observations. in addition ... | 2012 | 22579699 |
| molecular cloning, expression, and immunolocalization of protein disulfide isomerase in excretory-secretory products from clonorchis sinensis. | protein disulfide isomerase (pdi) is an essential catalyst of the endoplasmic reticulum with folding and chaperone activities in different biological systems. here, pdi of clonorchis sinensis (cspdi) was isolated from the cdna library of adult c. sinensis. the open reading frame contains 1,317 bp encoding 438 amino acids and shares 53 %, 49 %, and 43 % identity with pdi from bos taurus, homo sapiens, and schistosoma mansoni, respectively. two catalytic thioredoxin motifs cxxc were found in this ... | 2012 | 22538482 |
| identification and characterization of paramyosin from cyst wall of metacercariae implicated protective efficacy against clonorchis sinensis infection. | human clonorchiasis has been increasingly prevalent in recent years and results in a threat to the public health in epidemic regions, motivating current strategies of vaccines to combat clonorchis sinensis (c. sinensis). in this study, we identified c. sinensis paramyosin (cspmy) from the cyst wall proteins of metacercariae by proteomic approaches and characterized the expressed recombinant pet-26b-cspmy protein (101 kda). bioinformatics analysis indicated that full-length sequences of paramyosi ... | 2012 | 22470461 |
| 41.5-kda cathepsin l protease from clonorchis sinensis: expression, characterization, and serological reactivity of one excretory-secretory antigen. | cysteine proteases (cps) were associated with the pathogenicity and excystment of clonorchis sinensis. most of them were potential antigens for the immunodiagnosis of clonorchiasis. more researches on cps will let us know more about their functions, and further employ them for the development of more efficient diagnostic reagent and prevention strategies. in the current study, a full-length sequence encoding cathepsin l from c. sinensis (cscl41.5) was identified from our adult cdna library. bioi ... | 2012 | 22406988 |
| insights into scp/taps proteins of liver flukes based on large-scale bioinformatic analyses of sequence datasets. | scp/taps proteins of parasitic helminths have been proposed to play key roles in fundamental biological processes linked to the invasion of and establishment in their mammalian host animals, such as the transition from free-living to parasitic stages and the modulation of host immune responses. despite the evidence that scp/taps proteins of parasitic nematodes are involved in host-parasite interactions, there is a paucity of information on this protein family for parasitic trematodes of socio-ec ... | 2012 | 22384000 |
| parasites and malignancies, a review, with emphasis on digestive cancer induced by cryptosporidium parvum (alveolata: apicomplexa). | the international agency for research on cancer (iarc) identifies ten infectious agents (viruses, bacteria, parasites) able to induce cancer disease in humans. among parasites, a carcinogenic role is currently recognized to the digenetic trematodes schistosoma haematobium, leading to bladder cancer, and to clonorchis sinensis or opisthorchis viverrini, which cause cholangiocarcinoma. furthermore, several reports suspected the potential association of other parasitic infections (due to protozoan ... | 2012 | 22348213 |
| development of mitochondrial loop-mediated isothermal amplification for detection of the small liver fluke opisthorchis viverrini (opisthorchiidae; trematoda; platyhelminthes). | mitochondrial dna sequences offer major advantages over the more usual nuclear targets for loop-mediated isothermal amplification approaches (mito-lamp) because multiple copies occur in every cell. four lamp primers [f3, fip(f1c+f2), bip(b1c+b2), and b3] were designed based on the mitochondrial nad1 sequence of opisthorchis viverrini and used for a highly specific assay (mito-ovlamp) to distinguish dna of o. viverrini from that of another opisthorchiid (clonorchis sinensis) and other trematodes ... | 2012 | 22322346 |
| structural analysis of secretory phospholipase a2 from clonorchis sinensis: therapeutic implications for hepatic fibrosis. | hepatic fibrosis is a common complication of the infection by the parasite, clonorchis sinensis. there is a high incidence of this disease in the asian countries with an increased risk of conversion to cancer. a secretory phospholipase a(2) (pla(2)) enzyme from the parasite is implicated in the pathology. this is an attractive drug target in the light of extensive structural characterization of this class of enzyme. in this study, the structure of the enzyme was modeled based on its sequence hom ... | 2012 | 22215060 |
| the global epidemiology of clonorchiasis and its relation with cholangiocarcinoma. | this paper reviews the epidemiological status and characteristics of clonorchiasis at global level and the etiological relationship between clonorchis sinensis infection and cholangiocarcinoma (cca). a conservative estimation was made that 15 million people were infected in the world in 2004, of which over 85% distributed in china. the epidemiology of clonorchiasis is characterized by rising trend in its prevalence, variability among sexes and age, as well as endemicity in different regions. mor ... | 2012 | 23849183 |
| evaluation of recombinant sjlap and sjfbpa in detecting antibodies to schistosoma japonicum. | to investigate the early response of immunoglobulin g (igg) antibody responses to schistosoma japonicum infection in mice by using the recombinant proteins, s. japonicum leucine aminopeptidase (rsjlap) and s. japonicum fructose-1, 6-bisphosphate aldolase (rsjfbpa), and evaluate the potential of rsjlap and rsjfbpa in diagnosis as well as in assessment of therapeutic efficacy in human schistosomiasis. | 2011 | 24830194 |
| [current prevalence of clonorchis sinensis infection in endemic areas of china]. | to investigate the current prevalence of clonorchis sinensis infection in the known endemic areas of china. | 2008 | 24812810 |
| hilar biliary obstruction: malignant masquerades. | biliary obstruction at the hepatic hilus is most often due to malignancy. clinically, it can be very difficult to differentiate malignant from benign obstruction when a mass lesion is not evident on cross-sectional imaging. in this article, we describe three cases of benign biliary obstruction due to so-called malignant masquerades to familiarize the reader with the clinical, radiographic, and pathological features of these uncommon but important entities. | 2007 | 21326728 |
| epidemiology of helminth diseases: clonorchis sinensis (cobbold, 1875) looss, 1907 on taiwan (formosa). res rep mr005.09-1601.3.25. | | 1964 | 24547282 |
| use of purified antigen for detection of paragonimus westermani and clonorchis sinensis in peoples of hsinchu hsien, taiwan (formosa). | | 1960 | 24545964 |
| palaeoparasitology and palaeogenetics: review and perspectives for the study of ancient human parasites. | while some species of parasites can be identified to species level from archaeological remains using microscopy (i.e. enterobius vermicularis, clonorchis sinensis), others can only be identified to family or genus level as different species produce eggs with similar morphology (i.e. tænia sp. and echinococcus sp.). molecular and immunological approaches offer the possibility to provide more precise determination at the species level. they can also identify taxa when classic parasite markers such ... | 2017 | 28747239 |
| comparative analysis of immune effects in mice model: clonorchis sinensis cysteine protease generated from recombinant escherichia coli and bacillus subtilis spores. | clonorchiasis remains a nonnegligible public health problem in endemic areas. cysteine protease of clonorchis sinensis (cscp) plays indispensable roles in the parasitic physiology and pathology, and has been exploited as a promising drug and vaccine candidate. in recent years, development of spore-based vaccines against multiple pathogens has attracted many investigators' interest. in previous studies, the recombinant escherichia coli (bl21) and bacillus subtilis spores expressing cscp have been ... | 2017 | 28502017 |
| discovery of parasite eggs in archeological residence during the 15th century in seoul, korea. | during civil engineering construction near sejong-ro, jongro-ku, seoul, cultural sites were found that are thought to have been built in the 15th century. this area was home to many different people as well as the leaders of the yi dynasty. to gain further insight into the life styles of the inhabitants of the old capital, soil samples were collected from various areas such as toilets, water foundations, and drainage ways. parasite eggs were examined by microscopy after 5 g soil samples were reh ... | 2017 | 28719964 |
| immune responses induced by co-infection with capillaria hepatica in clonorchis sinensis-infected rats. | clonorchis sinensis and capillaria hepatica are zoonotic parasites that mainly infect the liver and cause serious liver disorders. however, immunological parameters induced by co-infection with these parasites remain unknown. in this study, for the first time, we investigated immunological profiles induced by co-infection with c. hepatica (ch) in c. sinensis (cs)-infected rats (sprague-dawley). rats were infected primarily with 50 metacercariae of c. sinensis; 4 weeks later, they were subsequent ... | 2017 | 28784187 |
| clonorchis sinensis infection and co-infection with the hepatitis b virus are important factors associated with cholangiocarcinoma and hepatocellular carcinoma. | to evaluate the contributions of clonorchis sinensis and hepatitis b virus to the development of cholangiocarcinoma (icc) and hepatocellular carcinoma (hcc), c. sinensis and hepatitis b virus infections in 20 clinical liver cancer cases from a c. sinensis- and hepatitis b virus-epidemic region were detected. eight cases of icc, 11 cases of hcc and one mixed icc and hcc case were verified by ct, pathological section and (or) observations during surgery. the c. sinensis infection was detected by s ... | 2017 | 28801696 |
| tlr2 signal influences the inos/no responses and worm development in c57bl/6j mice infected with clonorchis sinensis. | although the responses of inducible nitric oxide synthase (inos) and associated cytokine after clonorchis sinensis infection have been studied recently, their mechanisms remain incompletely understood. in this study, we investigated the effects of toll-like receptor 2 (tlr2) signals on inos/nitric oxide (no) responses after c. sinensis infection. we also evaluated the correlations between inos responses and worm development, which are possibly regulated by tlr2 signal. | 2017 | 28784165 |
| an essential role of n-terminal domain of copper chaperone in the enzymatic activation of cu/zn-superoxide dismutase. | cu/zn-superoxide dismutase (sod1) is an enzyme that disproportionates superoxide anion into hydrogen peroxide and molecular oxygen. the enzymatic activity of sod1 requires the binding of copper and zinc ions and also the formation of a conserved intramolecular disulfide bond. in a eukaryotic cell, a copper chaperone for sod1 (ccs) has been known to supply a copper ion and also introduce the disulfide bond into sod1; however, a mechanism controlling the ccs-dependent activation of sod1 remains ob ... | 2017 | 28780408 |
| hepatic iron overload is associated with hepatocyte apoptosis during clonorchis sinensis infection. | hepatic iron overload has been implicated in many liver diseases; however, whether it is involved in clonorchiasis remains unknown. the purpose of this study is to investigate whether clonorchis sinensis (c. sinensis) infection causes hepatic iron overload, analyze the relationship between the iron overload and associated cell apoptosis, so as to determine the role of excess iron plays in c. sinensis-induced liver injury. | 2017 | 28764657 |
| metacercaria infection status of fishborne zoonotic trematodes, except for clonorchis sinensis in fish from the heilongjiang province, china. | to investigate the metacercarial infections of fishborne zoonotic trematodes (fzt), a total of 6815 freshwater fish (in representing 13 species of 5 families) were collected from songhua river (n = 2636), nenjiang river (n = 1935), mudanjiang river (n = 301), and other lakes or ponds (n = 1943) in 36 representative regions in heilongjiang province, china, from august 2012 to december 2015. metacercariae of four fzt species, that is, clonorchis sinensis, metorchis orientalis, isthmiophora hortens ... | 2017 | 28737961 |
| prevalence of clonorchis sinensis metacercariae in fish from water systems of seomjin-gang (river). | the prevalence of clonorchis sinensis metacercariae (csmc) was examined in freshwater fish from the water systems of seomjin-gang (river), the republic of korea. total 1,604 fish from 7 local sites of seomjin-gang were examined by artificial digestion methods. the metacercariae of c. sinensis were detected in 102 (39.8%) out of 256 fish (14 species) from the upper reaches of seomjin-gang, i.e., osucheon (22.3% in 6 fish species) in imsil-gun, and seomjin-gang (63.9% in 9 fish species) in sunchan ... | 2017 | 28719955 |
| the roles of toll-like receptor 4 in the pathogenesis of pathogen-associated biliary fibrosis caused by clonorchis sinensis. | pathogen-associated biliary fibrosis (pabf) is a type of liver fibrosis characterized by injuries of cholangiocytes and extra cellular matrix (ecm) deposition around bile ducts caused by various bacteria, fungi, virus and parasites. recent studies show that tlr4 plays an important role in several other types of liver fibrosis, but the mechanism of tlr4 in pabf is yet really unclear. in the present study, a pabf mouse model was established by a trematode infection-clonorchis sinensis which dwells ... | 2017 | 28634394 |
| clonorchis sinensis lysophospholipase a upregulates il-25 expression in macrophages as a potential pathway to liver fibrosis. | liver fibrosis is an excessive wound-healing reaction that requires the participation of inflammatory cells and hepatic stellate cells (hscs). the pathogenesis of liver fibrosis caused by viruses and alcohol has been well characterized, but the molecular mechanisms underlying liver fibrosis induced by the liver fluke clonorchis sinensis are poorly understood. lysophospholipase a (lysopla), which deacylates lysophospholipids, plays a critical role in mediating the virulence and pathogenesis of pa ... | 2017 | 28623940 |
| lineage-specific expansion and loss of tyrosinase genes across platyhelminths and their induction profiles in the carcinogenic oriental liver fluke, clonorchis sinensis. | tyrosinase provides an essential activity during egg production in diverse platyhelminths by mediating sclerotization of eggshells. in this study, we investigated the genomic and evolutionary features of tyrosinases in parasitic platyhelminths whose genomic information is available. a pair of paralogous tyrosinases was detected in most trematodes, whereas they were lost in cyclophyllidean cestodes. a pseudophyllidean cestode displaying egg biology similar to that of trematodes possessed an ortho ... | 2017 | 28583212 |
| clonorchis sinensis granulin: identification, immunolocalization, and function in promoting the metastasis of cholangiocarcinoma and hepatocellular carcinoma. | long-term infections by clonorchis sinensis are associated with cholangitis, cholecystitis, liver fibrosis, cirrhosis, and even liver cancer. molecules from the worm play vital roles in disease progress. in the present study, we identified and explored molecular characterization of c. sinensis granulin (csgrn), a growth factor-like protein from c. sinensis excretory/secretory products (csesps). | 2017 | 28545547 |
| clonorchis sinensis antigens alter hepatic macrophage polarization in vitro and in vivo. | clonorchis sinensis infection elicits hepatic inflammation, which can lead to cholangitis, periductal hepatic fibrosis, liver cirrhosis, and even cholangiocarcinoma. hepatic macrophages are an intrinsic element of both innate and acquired immunity. this study was conducted to demonstrate the dynamics of hepatic macrophage polarization during c. sinensis infection in mice and to identify factors regulating this polarization. treatment of hepatic macrophages isolated from normal mice with c. sinen ... | 2017 | 28542159 |
| [genomics and transcriptomics of the chinese liver fluke clonorchis sinensis (opisthorchiidae, trematoda)]. | the review summarizes the results of first genomic and transcriptomic investigations of the liver fluke clonorchis sinensis (opisthorchiidae, trematoda). the studies mark the dawn of the genomic era for opisthorchiids, which cause severe hepatobiliary diseases in humans and animals. their results aided in understanding the molecular mechanisms of adaptation to parasitism, parasite survival in mammalian biliary tracts, and genome dynamics in the individual development and the development of paras ... | 2017 | 28537229 |
| epidemiological and clinical parameters features of patients with clonorchiasis in the geum river basin, republic of korea. | this study was conducted to evaluate the infection rates of clonorchis sinensis and laboratory findings in infected people. the 3,167 fecal samples, from nine villages in okcheon-gun, were examined. | 2017 | 28529523 |
| codon usage patterns of tyrosinase genes in clonorchis sinensis. | codon usage bias (cub) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. in this study, genetic indices associated with cub, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the clonorchis sinensis tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. the ... | 2017 | 28506040 |
| structural insights into a 20.8-kda tegumental-allergen-like (tal) protein from clonorchis sinensis. | survival of clonorchis sinensis, a cause of human clonorchiasis, requires tegument proteins, which are localized to the tegumental outer surface membrane. these proteins play an important role in a host response and parasite survival. thus, these proteins are interesting molecular targets for vaccine and drug development. here, we have determined two crystal structures of the calmodulin like domain (amino acid [aa] positions 1-81) and dynein light chain (dlc)-like domain (aa 83-177) of a 20.8-kd ... | 2017 | 28496122 |
| first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar. | opisthorchis viverrini is endemic in the south east asian region, especially in cambodia, lao people's democratic republic, vietnam and thailand, but there have been no previous records from myanmar. during stool surveys of rural populations in three regions of lower myanmar, opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. dna was extracted from these positive stool samples and a portion o ... | 2017 | 28472153 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis, and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsis ... | 2015 | 28741473 |