| biosynthesis of enantiopure (s)-3-hydroxybutyric acid in metabolically engineered escherichia coli. | a biosynthetic pathway for the production of (s)-3-hydroxybutyric acid (s3hb) from glucose was established in recombinant escherichia coli by introducing the beta-ketothiolase gene from ralstonia eutropha h16, the (s)-3-hydroxybutyryl-coa dehydrogenase gene from r. eutropha h16, or clostridium acetobutylicum atcc824, and the 3-hydroxyisobutyryl-coa hydrolase gene from bacillus cereus atcc14579. artificial operon consisting of these genes was constructed and was expressed in e. coli bl21 (de3) co ... | 2008 | 18461320 |
| microbial utilization and biopolyester synthesis of bagasse hydrolysates. | cellulosic biomass is a potentially inexpensive renewable feedstock for the biorefineries of fuels, chemicals and materials. sugarcane bagasse was pretreated in dilute acid solution under moderately severe conditions, releasing sugars and other hydrolysates including volatile organic acids, furfurals and acid soluble lignin. utilization of the hydrolysates by an aerobic bacterium, ralstonia eutropha, was investigated to determine if the organic inhibitors can be removed for potential recycling a ... | 2008 | 18474421 |
| diversity of microbial toluene degradation pathways. | | 2008 | 18485280 |
| ralstonia eutropha h16 flagellation changes according to nutrient supply and state of poly(3-hydroxybutyrate) accumulation. | two-dimensional polyacrylamide gel electrophoresis (2d page), in combination with matrix-assisted laser desorption ionization-time of flight analysis, and the recently revealed genome sequence of ralstonia eutropha h16 were employed to detect and identify proteins that are differentially expressed during different phases of poly(3-hydroxybutyric acid) (phb) metabolism. for this, a modified protein extraction protocol applicable to phb-harboring cells was developed to enable 2d page-based proteom ... | 2008 | 18502919 |
| mammalian pro-apoptotic bax gene enhances tobacco resistance to pathogens. | emerging evidence suggests that plants and animals may share certain biochemical commonalities in apoptosis, or programmed cell death (pcd) pathways, though plants lack key animal apoptosis related genes. in plants, pcd has many important functions including a role in immunity and resistance to pathogen infection. in this study, a rice phenylalanine ammonia-lyase promoter is used to regulate the expression of the mouse pro-apoptotic bax gene in transgenic tobacco plants. ectopic expression of th ... | 2008 | 18509654 |
| extensive biodegradation of polychlorinated biphenyls in aroclor 1242 and electrical transformer fluid (askarel) by natural strains of microorganisms indigenous to contaminated african systems. | evidence for substantial aerobic degradation of aroclor 1242 and askarel fluid by newly characterized bacterial strains belonging to the enterobacter, ralstonia and pseudomonas genera is presented. the organisms exhibited degradative activity in terms of total pcb/askarel degradation, degradation of individual congeners and diversity of congeners attacked. maximal degradation by the various isolates of askarel ranged from 69% to 86% whereas, aroclor 1242, with the exception of ralstonia sp. sa-4 ... | 2008 | 18550146 |
| fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients. | the accurate and rapid identification of bacteria isolated from the respiratory tract of patients with cystic fibrosis (cf) is critical in epidemiological studies, during intrahospital outbreaks, for patient treatment, and for determination of therapeutic options. while the most common organisms isolated from sputum samples are pseudomonas aeruginosa, staphylococcus aureus, and haemophilus influenzae, in recent decades an increasing fraction of cf patients has been colonized by other nonfermenti ... | 2008 | 18550747 |
| gradients of coastal fish farm effluents and their effect on coral reef microbes. | coastal milkfish (chanos chanos) farming may be a source of organic matter enrichment for coral reefs in bolinao, republic of the philippines. interactions among microbial communities associated with the water column, corals and milkfish feces can provide insight into the ecosystem's response to enrichment. samples were collected at sites along a transect that extended from suspended milkfish pens into the coral reef. water was characterized by steep gradients in the concentrations of dissolved ... | 2008 | 18557772 |
| outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation. | bacterial biodegradation of hydrocarbons, an important process for environmental remediation, requires the passage of hydrophobic substrates across the cell membrane. here, we report crystal structures of two outer membrane proteins, pseudomonas putida todx and ralstonia pickettii tbux, which have been implicated in hydrocarbon transport and are part of a subfamily of the fadl fatty acid transporter family. the structures of todx and tbux show significant differences with those previously determ ... | 2008 | 18559855 |
| transcriptional responses of arabidopsis thaliana during wilt disease caused by the soil-borne phytopathogenic bacterium, ralstonia solanacearum. | bacterial wilt is a common disease that causes severe yield and quality losses in many plants. in the present study, we used the model ralstonia solanacearum-arabidopsis thaliana pathosystem to study transcriptional changes associated with wilt disease development. susceptible col-5 plants and rrs1-r-containing resistant nd-1 plants were root-inoculated with r. solanacearum strains harbouring or lacking the matching popp2 avirulence gene. gene expression was marginally affected in leaves during ... | 2008 | 18596930 |
| expression of the ethylene response factor gene tsrf1 enhances abscisic acid responses during seedling development in tobacco. | ethylene response factor (erf) proteins function as multiple regulators in the interaction of different stress-responsive pathways. during investigating the interaction of ethylene and abscisic acid (aba) pathways, several gcc-box-binding repressors of erf proteins have been reported to repress both ethylene- and aba-related responses, but it is unclear how gcc-box-binding activator erf proteins are involved in this interaction. previously, we isolated an erf protein tomato stress-responsive fac ... | 2008 | 18597110 |
| stereoselective bioreduction of bulky-bulky ketones by a novel adh from ralstonia sp. | ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase rasadh from ralstonia sp. dsm 6428 overexpressed in e. coli. | 2008 | 18597534 |
| structure of the oligosaccharide chain of the sr-type lipopolysaccharide of ralstonia solanacearum toudk-2. | aiming at improving classification and taxonomy of gram-negative phytopathogenic bacteria, we studied the structure of the lipopolysaccharide of ralstonia solanacearum. mild acid hydrolysis of the lipopolysaccharide of strain toudk-2 followed by gel chromatography resulted in an o-polysaccharide and two oligosaccharide fractions. the smallest-size oligosaccharide fraction was studied by sugar analysis, high-resolution electrospray ionization mass spectrometry, and, after fractionation by anion-e ... | 2008 | 18613725 |
| genomic analyses of transport proteins in ralstonia metallidurans. | ralstonia (wautersia, cupriavidus) metallidurans (rme) is better able to withstand high concentrations of heavy metals than any other well-studied organism. this fact renders it a potential agent of bioremediation as well as an ideal model organism for understanding metal resistance phenotypes. we have analysed the genome of rme for genes encoding homologues of established and putative transport proteins; 13% of all genes in rme encode such homologues. nearly one-third of the transporters identi ... | 2005 | 18629293 |
| biological control against bacterial wilt and colonization of mulberry by an endophytic bacillus subtilis strain. | forty-five bacterial isolates were collected from surface-sterilized leaves of mulberry (morus alba l.). by screening their antagonistic activities against ralstonia solanacearum in vitro, four isolates showed a remarkable inhibitory effect. the evaluation of the antagonistic strains against bacterial wilt of mulberry indicated that the strain lu144 effectively reduced disease incidence. in the greenhouse, lu144 displayed effective biological control against bacterial wilt of mulberry when it wa ... | 2008 | 18631174 |
| an amino acid substitution at position 740 in sigma70 of ralstonia solanacearum strain oe1-1 affects its in planta growth. | growth of ralstonia solanacearum strain oe1-1 in roots after invasion is required for virulence. an arg740cys substitution in sigma(70) of oe1-1 resulted in loss of in planta growth and virulence. the negative dominance of mutant sigma(70) over the wild-type protein suggested that the amino acid substitution may affect the in planta growth of oe1-1, leading to a lack of virulence. | 2008 | 18641146 |
| hydrogen production under aerobic conditions by membrane-bound hydrogenases from ralstonia species. | studies have been carried out to establish the ability of o2-tolerant membrane-bound [nife] hydrogenases (mbh) from ralstonia sp. to catalyze h2 production in addition to h2 oxidation. these hydrogenases are not noted for h2-evolution activity, and this is partly due to strong product inhibition. however, when adsorbed on a rotating disk graphite electrode the enzymes produce h2 efficiently, provided the h2 product is continuously removed by rapidly rotating the electrode and flowing n2 through ... | 2008 | 18661984 |
| natural transformation in the ralstonia solanacearum species complex: number and size of dna that can be transferred. | ralstonia solanacearum is a widely distributed phytopathogenic bacterium that is known to invade more than 200 host species, mainly in tropical areas. reference strain gmi1000 is naturally transformable at in vitro and also in planta conditions and thus has the ability to acquire free exogenous dna. we tested the ubiquity and variability of natural transformation in the four phylotypes of this species complex using 55 strains isolated from different hosts and geographical regions. eighty per cen ... | 2008 | 18662313 |
| reduction of petroleum hydrocarbons and toxicity in refinery wastewater by bioremediation. | the aim of the study was to investigate petroleum waste remediation and toxicity reduction by five bacterial strains: ralstonia picketti srs (bp-20), alcaligenes piechaudii srs (czor l-1b), bacillus subtilis (i'-1a), bacillus sp. (t-1), and bacillus sp. (t'-1), previously isolated from petroleum-contaminated soils. petroleum hydrocarbons were significantly degraded (91%) by the mixed bacterial cultures in 30 days (reaching up to 29% in the first 72 h). similarly, the toxicity of the biodegraded ... | 2008 | 18663400 |
| [glycopolymers of microorganisms: molecular relationship between structure, function, and biological activity]. | the results of the department of biochemistry investigations during the recent 5 years are presented. for the first time the structures of o-specific polysaccharides of lipopolysaccharides of representatives of enterobacteriaceae new genus--rahnella aquatilis have been established. the structures are represented by linear or branched mono-, tri- and hexasaccharides. as well as in other enterobacteriaceae representatives only one hydrohylated acid--3-hydroxytetradecanoic has been established in l ... | 2008 | 18663920 |
| brassica juncea chitinase bjchi1 inhibits growth of fungal phytopathogens and agglutinates gram-negative bacteria. | brassica juncea bjchi1 is a plant chitinase with two chitin-binding domains. its expression, induced in response to wounding, methyl jasmonate treatment, aspergillus niger infection, and caterpillar pieris rapae feeding, suggests that it plays a role in defence. in this study, to investigate the potential of using bjchi1 in agriculture, pichia-expressed bjchi1 and its deletion derivatives that lack one or both chitin-binding domains were tested against phytopathogenic fungi and bacteria. transpl ... | 2008 | 18669819 |
| pseudomonas and aeromonas isolates from domestic reptiles and study of their antimicrobial in vitro sensitivity. | | 2008 | 18683065 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| expression of a class 1 hemoglobin gene and production of nitric oxide in response to symbiotic and pathogenic bacteria in lotus japonicus. | symbiotic nitrogen fixation by the collaboration between leguminous plants and rhizobia is an important system in the global nitrogen cycle, and some molecular aspects during the early stage of host-symbiont recognition have been revealed. to understand the responses of a host plant against various bacteria, we examined expression of hemoglobin (hb) genes and production of nitric oxide (no) in lotus japonicus after inoculation with rhizobia or plant pathogens. when the symbiotic rhizobium mesorh ... | 2008 | 18700822 |
| transposon mutagenesis reveals differential pathogenesis of ralstonia solanacearum on tomato and arabidopsis. | ralstonia solanacearum causes a deadly wilting disease on a wide range of crops. to elucidate pathogenesis of this bacterium in different host plants, we set out to identify r. solanacearum genes involved in pathogenesis by screening random transposon insertion mutants of a highly virulent strain, pss190, on tomato and arabidopsis thaliana. mutants exhibiting various decreased virulence levels on these two hosts were identified. sequence analysis showed that most, but not all, of the identified ... | 2008 | 18700830 |
| the arabidopsis transcription factor wrky27 influences wilt disease symptom development caused by ralstonia solanacearum. | wrky transcription factors play a key role in modulating the plant defense transcriptome. here we show that the arabidopsis mutant wrky27-1, which lacks a functional wrky27 transcription factor, showed delayed symptom development in response to the bacterial wilt pathogen ralstonia solanacearum. additionally, wrky27-1 plants did not express pr marker genes upon infection, as also observed in resistant nd-1 plants. spatial expression of wrky27 correlated well with the route of bacterial infection ... | 2008 | 18702671 |
| a putative rna-binding protein has a role in virulence in ralstonia solanacearum gmi1000. | molecular signals contained in root exudates are thought to induce specific transcriptional changes in bacteria within the rhizosphere, promoting the expression of genes associated with rhizosphere function. we have previously used pseudomonas aeruginosa pao1 as a model organism to identify novel genes with roles in rhizosphere competitiveness through analysis of those which showed altered transcription in response to root exudates. one such gene, pa4753, encodes a putative rna-binding protein. ... | 2008 | 18705885 |
| rd19, an arabidopsis cysteine protease required for rrs1-r-mediated resistance, is relocalized to the nucleus by the ralstonia solanacearum popp2 effector. | bacterial wilt, a disease impacting cultivated crops worldwide, is caused by the pathogenic bacterium ralstonia solanacearum. popp2 (for pseudomonas outer protein p2) is an r. solanacearum type iii effector that belongs to the yopj/avrrxv protein family and interacts with the arabidopsis thaliana resistant to ralstonia solanacearum 1-r (rrs1-r) resistance protein. rrs1-r contains the toll/interleukin1 receptor-nucleotide binding site-leu-rich repeat domains found in several cytoplasmic r protein ... | 2008 | 18708476 |
| characterization of new isolated ralstonia eutropha strain a-04 and kinetic study of biodegradable copolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate) production. | a new isolated bacterial strain a-04 capable of producing high content of polyhydroxyalkanoates (phas) was morphologically and taxonomically identified based on biochemical tests and 16s rrna gene analysis. the isolate is a member of the genus ralstonia and close to ralstonia eutropha. hence, this study has led to the finding of a new and unexplored r. eutropha strain a-04 capable of producing phas with reasonable yield. the kinetic study of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [p(3hb-co ... | 2008 | 18712546 |
| characterization of biofilm formation on a humic material. | biofilms are major sites of carbon cycling in streams. therefore, it is crucial to improve knowledge about biofilms' structure and microbial composition to understand their contribution in the self-purification of surface water. the present work intends to study biofilm formation in the presence of humic substances (hss) as a carbon source. two biofilm flowcells were operated in parallel; one with synthetic stream water, displaying a background carbon concentration of 1.26+/-0.84 mg l(-1), the o ... | 2008 | 18712549 |
| polyhydroxyalkanoate (pha) production using waste vegetable oil by pseudomonas sp. strain dr2. | to produce polyhydroxyalkanoate (pha) from inexpensive substrates by bacteria, vegetable-oil-degrading bacteria were isolated from a rice field using enrichment cultivation. the isolated pseudomonas sp. strain dr2 showed clear orange or red spots of accumulated pha granules when grown on phosphate and nitrogen limited medium containing vegetable oil as the sole carbon source and stained with nile blue a. up to 37.34% (w/w) of intracellular pha was produced from corn oil, which consisted of three ... | 2008 | 18756101 |
| polyhydroxyalkanoate film formation and synthase activity during in vitro and in situ polymerization on hydrophobic surfaces. | in vitro and in situ enzymatic polymerization of polyhydroxyalkanoate (pha) on two hydrophobic surfaces, a highly oriented pyrolytic graphite (hopg) and an alkanethiol self-assembled monolayer (sam), was studied by atomic force microscopy (afm) and quartz crystal microbalance (qcm), using purified ralstonia eutropha pha synthase (phac(re)) as a biocatalyst. (r)-specific enoyl-coa hydratase was used to prepare r-enantiomer monomers [(r)-3-hydroxyacyl-coa] with an acyl chain length of 4-6 carbon a ... | 2008 | 18771315 |
| growth of three bacteria in arsenic solution and their application for arsenic removal from wastewater. | the present paper compares the arsenic removal capacities of three bacterial strains namely, ralstonia eutropha mtcc 2487, pseudomonas putida mtcc 1194 and bacillus indicus mtcc 4374 form wastewater (simulated acid mine drainage) containing arsenic (as(iii):as(v)::1:1), fe, mn, cu and zn in the concentration of 15 mg/l, 10 mg/l, 2 mg/l, 5 mg/l and 10 mg/l respectively, in bulk liquid phase. growth patterns of these bacteria in presence of arsenic in solution as well as under starvation have also ... | 2008 | 18792057 |
| evaluating the ability of polyhydroxyalkanoate synthase mutants to produce p(3hb-co-3ha) from soybean oil. | polyhydroxyalkanoate (pha) synthase from pseudomonas sp 61-3 (phac1(ps)) is able to synthesize p(3hb-co-3ha), consisting of a 3hb unit and medium-chain-length 3ha units of 6-12 carbon atoms. expression vectors encoding 76 phac1(ps) mutants with an amino acid replacement at position 130, 325, 477 or 481 were individually introduced into ralstonia eutropha. the mutant enzyme genes were evaluated in terms of their abilities to synthesize p(3hb-co-3ha) using soybean oil as a carbon source. 20 mutant ... | 2009 | 18798179 |
| simple enrichment and independent cultures to expand bacterial community analysis from gedongsongo hot spring. | denaturing gradient gel electrophoresis was used to identify bacterial communities from gedongsongo hot spring, indonesia, by culture-independent and -dependent procedures. predominant species were found to be closely related to beta-proteobacteria, particularly from genus ralstonia and delftia. other species present include alpha- and gamma-proteobacteria and thermus groups. some of the strains showed unique 16s rdna sequences. | 2008 | 18804068 |
| structure of bacterial glutathione-s-transferase maleyl pyruvate isomerase and implications for mechanism of isomerisation. | maleyl pyruvate isomerase (mpi) is a bacterial glutathione s-transferase (gst) from the pathway for degradation of naphthalene via gentisate that enables the bacterium ralstonia to use polyaromatic hydrocarbons as a sole carbon source. genome sequencing projects have revealed the presence of large numbers of gsts in bacterial genomes, often located within gene clusters encoding the degradation of different aromatic compounds. this structure is therefore an example of this under-represented class ... | 2008 | 18824004 |
| a novel goose-type lysozyme gene with chitinolytic activity from the moderately thermophilic bacterium ralstonia sp. a-471: cloning, sequencing, and expression. | in this study, we cloned the gene encoding goose-type (g-type) lysozyme with chitinase (ra-chic) activity from ralstonia sp. a-471 genomic dna library. this is the first report of another type of chitinase after the previously reported chitinases chia (ra-chia) and chib (ra-chib) in the chitinase system of the moderately thermophilic bacterium, ralstonia sp. a-471 and also the first such data in ralstonia sp. g-type lysozyme gene. it consisted of 753 bp nucleotides, which encodes 251 amino acids ... | 2009 | 18825379 |
| characterization of new oxidation products of 9h-carbazole and structure related compounds by biphenyl-utilizing bacteria. | 9h-carbazole and its derivatives are useful for versatile pharmacological applications. to obtain different derivatives of 9h-carbazole, 24 isolates of biphenyl-utilizing bacteria have been investigated regarding their ability to produce hydroxylated 9h-carbazole metabolites. our analyses showed that 9h-carbazole was primarily converted into 9h-carbazol-1-ol (15 strains) and 9h-carbazol-3-ol (9 strains), while carbazol-9-ol was formed as a minor product (12 strains). the formation of 9h-carbazol ... | 2009 | 18836714 |
| microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge. | the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ... | 2008 | 18838186 |
| adsorption characteristics of p(3hb) depolymerase as evaluated by surface plasmon resonance and atomic force microscopy. | molecular recognition of poly[(r)-3-hydroxybutyrate] (p(3hb)) depolymerase from ralstonia pickettii t1 to the surfaces of biodegradable aliphatic polyesters such as p(3hb) and poly(l-lactic acid) (plla) was examined from the viewpoints of kinetics and dynamics. to determine the kinetic parameters on the interaction between the substrate-binding domain (sbd) of p(3hb) depolymerase and various polymer substrates with different chemical structures, surface plasmon resonance (spr) measurements were ... | 2008 | 18939805 |
| ethylene-insensitive tobacco shows differentially altered susceptibility to different pathogens. | abstract transgenic tobacco plants (tetr) expressing the mutant etr1-1 gene from arabidopsis thaliana are insensitive to ethylene and develop symptoms of wilting and stem rot when grown in nonautoclaved soil. several isolates of fusarium, thielaviopsis, and pythium were recovered from stems of diseased tetr plants. inoculation with each of these isolates of 6-week-old plants growing in autoclaved soil caused disease in tetr plants but not in nontransformed plants. also, when 2-week-old seedlings ... | 2003 | 18943162 |
| colonization of pythium oligandrum in the tomato rhizosphere for biological control of bacterial wilt disease analyzed by real-time pcr and confocal laser-scanning microscopy. | it recently has been reported that the non-plant-pathogenic oomycete pythium oligandrum suppresses bacterial wilt caused by ralstonia solanacearum in tomato. as one approach to determine disease-suppressive mechanisms of action, we analyzed the colonization of p. oligandrum in rhizospheres of tomato using real-time polymerase chain reaction (pcr) and confocal laser-scanning microscopy. the real-time pcr specifically quantified p. oligandrum in the tomato rhizosphere that is reliable over a range ... | 2008 | 18943195 |
| integrated approach for detection of nonculturable cells of ralstonia solanacearum in asymptomatic pelargonium spp. cuttings. | ralstonia solanacearum (biovar 2, race 3) is a soil and water-borne pathogen that causes serious diseases in several solanaceous hosts. it can also infect geranium plants, posing an important threat to their culture when latently infected cuttings are imported from countries where the pathogen is endemic. r. solanacearum can be present in very low numbers in asymptomatic geranium cuttings, and/or in a particular stressed physiological state that escapes direct isolation on the solid media usuall ... | 2008 | 18943214 |
| comparative behavior of ralstonia solanacearum biovar 2 in diverse plant species. | ralstonia solanacearum causes bacterial wilt in numerous plant species worldwide. although biovar 2 mostly affects solanaceous crops, identification of new hosts remains a matter of concern since there is still no clear-cut distinction between host and nonhost plants. in this work we provide data based on histological studies on the status of 20 plant species, most of them of potential interest in crop rotation. plants were watered with a beta-glucuronidase-expressing derivative of r. solanacear ... | 2008 | 18943239 |
| survival of ralstonia solanacearum biovar 2, the causative agent of potato brown rot, in field and microcosm soils in temperate climates. | abstract after outbreaks of potato brown rot in three different fields in the netherlands, the fate of the brown rot pathogen, ralstonia solanacearum biovar 2, was monitored in soil by immunofluorescence colony staining (ifc) supported by r. solanacearum division-2 specific polymerase chain reaction. in selected areas of all fields, the r. solanacearum population densities were initially on the order 10(4) to 10(6) per g of topsoil. these population densities then declined progressively over tim ... | 2000 | 18943377 |
| diversity of ralstonia solanacearum infecting eggplant in the philippines. | abstract the diversity of ralstonia solanacearum strains isolated from eggplant (solanum melongena) grown in five provinces of the philippine island group of luzon was assessed using a recently described hierarchical system. all strains keyed to race 1, biovar 3 or 4. phylotype-specific multiplex polymerase chain reaction (pcr) indicated that, like most other strains of asian origin, all the strains in our philippine collection belong to phylotype i. taxometric and phylogenetic analyses of parti ... | 2007 | 18943517 |
| resistance enhancement of transgenic tomato to bacterial pathogens by the heterologous expression of sweet pepper ferredoxin-i protein. | abstract expression of a foreign gene to enhance plant disease resistance to bacterial pathogens is a favorable strategy. it has been demonstrated that expressing sweet pepper ferredoxin-i protein (pflp) in transgenic plants can enhance disease resistance to bacterial pathogens that infect leaf tissue. in this study, pflp was applied to protect tomato (lycopersicon esculentum cv. cherry cln1558a) from the root-infecting pathogen, ralstonia solanacearum. independent r. solanacearum resistant t(1) ... | 2007 | 18943629 |
| detection of ralstonia solanacearum by loop-mediated isothermal amplification. | ralstonia solanacearum is a pathogenic bacterium that causes wilt in over 200 plant species. here we report a rapid and sensitive detection of r. solanacearum using an isothermal method for copying dna known as loop-mediated amplification (lamp). a set of four primers was designed to replicate the gene coding for the flagellar subunit, flic, and conditions for detection were optimized to complete in 60 min at 65 degrees c. magnesium pyrophosphate resulting from the amplification reaction could b ... | 2008 | 18943743 |
| the low-temperature-induced viable-but-nonculturable state affects the virulence of ralstonia solanacearum biovar 2. | abstract the physiology and virulence of ralstonia solanacearum biovar 2 strain 1609, kept in water at 4 and 20 degrees c, were studied. at 20 degrees c, total cell and plate count (colony forming units; cfu) numbers were similar, between log 5.03 and log 5.55 cfu, and log 5.03 and log 5.51 cells per ml, at days 0 and 132, respectively. however, cfu in the cultures kept at 4 degrees c dropped from log 6.78 cfu/ml at day 0 to below detection after 84 days. the presence of catalase in the agar res ... | 2004 | 18943764 |
| genetic diversity of japanese strains of ralstonia solanacearum. | abstract the genetic diversity of 74 japanese strains of ralstonia solanacearum was assessed by pathogenicity tests and the repetitive sequencebased polymerase chain reaction (rep-pcr) fingerprint method. based on their genomic fingerprints, biovar n2 strains were divided into two distinct groups, one consisting of potato isolates belonging to race 3, and the other consisting of tomato, eggplant, pepper, and tobacco isolates belonging to race 1. biovar 3 strains had low average similarity and we ... | 2001 | 18943853 |
| interactions between strains of 2,4-diacetylphloroglucinol-producing pseudomonas fluorescens in the rhizosphere of wheat. | abstract strains of fluorescent pseudomonas spp. that produce the antibiotic 2,4-diacetylphoroglucinol (2,4-dapg) are among the most effective rhizobacteria controlling diseases caused by soilborne pathogens. the genotypic diversity that exists among 2,4-dapg producers can be exploited to improve rhizosphere competence and biocontrol activity. knowing that d-genotype 2,4-dapg-producing strains are enriched in some take-all decline soils and that p. fluorescens q8r1-96, a representative d-genotyp ... | 2003 | 18943865 |
| isolation and characterization of a novel arabidopsis thaliana mutant unable to develop wilt symptoms after inoculation with a virulent strain of ralstonia solanacearum. | abstract to characterize host genes required for a compatible interaction, we identified a novel recessive arabidopsis thaliana mutant, nws1 (no wilt symptoms), that failed to develop wilt symptoms in response to virulent strains of the phytopathogenic bacterium, ralstonia solanacearum. the absence of wilting in nws1 plants was not correlated with a cell death phenotype or a constitutive expression of salicylic acid-, jasmonic acid- or ethylene-associated genes. in addition, this mutation, which ... | 2004 | 18943977 |
| behavior of ralstonia solanacearum race 3 biovar 2 during latent and active infection of geranium. | abstract southern wilt of geraniums (pelargonium hortorum), caused by the soilborne bacterium ralstonia solanacearum race 3 biovar 2 (r3bv2), has inflicted significant economic losses when geranium cuttings latently infected with this quarantine pest were imported into the united states. little is known about the interaction between r. solanacearum and this ornamental host. using uw551, a virulent r3bv2 geranium isolate from a kenyan geranium, we characterized development of southern wilt diseas ... | 2005 | 18943982 |
| molecular diversity of ralstonia solanacearum isolated from ginger in hawaii. | abstract the genetic diversity of ralstonia solanacearum strains isolated from ginger (zingiber officinale) growing on the island of hawaii was determined by analysis of amplified fragment length polymorphisms (aflps). initially 28 strains of r. solanacearum collected from five host plant species worldwide were analyzed by aflp. a second analysis was conducted on 55 r. solanacearum strains collected from three ginger farms along the hamakua coast of hawaii, the principle area of ginger cultivati ... | 2003 | 18944096 |
| delayed symptom development in ein2-1, an arabidopsis ethylene-insensitive mutant, in response to bacterial wilt caused by ralstonia solanacearum. | abstract wilt disease caused by the phytopathogenic bacterium ralstonia solanacearum is poorly understood at the molecular level. the possible roles of salicylic acid, jasmonic acid, and ethylene, compounds commonly associated with the plant response to pathogens, in wilt symptom development were investigated using various arabidopsis thaliana mutants in a col-0 background, an ecotype that develops wilt symptoms in response to the virulent gmi1000 strain. following root inoculation, wilt symptom ... | 2002 | 18944225 |
| an oligonucleotide array for the identification and differentiation of bacteria pathogenic on potato. | abstract oligonucleotides, 16 to 24 bases long, were selected from the 3' end of the 16s gene and the 16s-23s intergenic spacer regions of bacteria pathogenic on potato, including clavibacter michiganensis subsp. sepedonicus, ralstonia solanacearum, and the pectolytic erwinias, including erwinia carotovora subsp. atroseptica and carotovora and e. chrysanthemi. oligonucleotides were designed and formatted into an array by pin spotting on nylon membranes. genomic dna from bacterial cultures was am ... | 2003 | 18944335 |
| spatial-temporal and quantitative analysis of growth and eps i production by ralstonia solanacearum in resistant and susceptible tomato cultivars. | abstract one susceptible and two resistant cultivars of tomato were tested for differences in infection by ralstonia solanacearum and for the subsequent multiplication, colonization, and production of the wilt-inducing virulence factor, exopolysaccharide i (eps i). bacterial ingress into the taproot was fastest in the susceptible cv. marion, followed by the resistant cvs. l285 (fivefold slower) and hawaii 7996 (15-fold slower). once inside the taproot, r. solanacearum colonized, to some extent, ... | 1999 | 18944650 |
| a single locus leads to resistance of arabidopsis thaliana to bacterial wilt caused by ralstonia solanacearum through a hypersensitive-like response. | abstract strains of ralstonia solanacearum have been shown to cause bacterial wilt in some, but not all, ecotypes of arabidopsis thaliana. we demonstrate here that after inoculation of the leaves of resistant ecotype s96 with r. solanacearum strain ps95 necrosis around the inoculation site rapidly appeared and no further symptoms developed in the plant. leaves of susceptible ecotype n913 completely wilted 7 days after inoculation with ps95, and symptoms spread systemically throughout the whole p ... | 1999 | 18944680 |
| comparison of ethylene production by pseudomonas syringae and ralstonia solanacearum. | abstract strains of pseudomonas syringae pv. pisi and ralstonia solanacearum produced ethylene at rates 20- and 200-fold lower, respectively, than strains of p. syringae pvs. cannabina, glycinea, phaseolicola, and sesami. in the current study, we investigated which ethylene biosynthetic pathways were used by p. syringae pv. pisi and r. solanacearum. neither the activity of an ethylene-forming enzyme nor a corresponding efe gene homolog could be detected in r. solanacearum, suggesting synthesis o ... | 1999 | 18944747 |
| variation in genotype and aggressiveness of ralstonia solanacearum race 1 isolated from tomato in taiwan. | abstract a population of ralstonia solanacearum race 1 from tomato (lycopersicon esculentum) was analyzed for genetic polymorphism and aggressiveness on tomato. the 46 strains were collected from main tomato-growing areas in taiwan. genetic analysis was achieved by two polymerase chain reaction (pcr)-based methods: rep-, eric-, and box-pcr (collectively as rep-pcr) and random amplified polymorphic dna (rapd) techniques. rapd (with three 10-mers) and rep-pcr revealed 35 and 30 haplotypes, respect ... | 1999 | 18944778 |
| development of specific recombinant monoclonal antibodies against the lipopolysaccharide of ralstonia solanacearum race 3. | abstract recombinant single-chain antibodies (scfvs) against the lipopolysaccharide of ralstonia solanacearum (biovar 2, race 3) were successfully selected by phage display from a large combinatorial antibody library. characterization with regard to cross-reaction and use in routine immunoassays showed that the selected antibodies had improved characteristics when compared with the polyclonal antiserum that is currently used for brown rot diagnosis of potato in the netherlands. the isolated mono ... | 1998 | 18944885 |
| expression of human lactoferrin cdna confers resistance to ralstonia solanacearum in transgenic tobacco plants. | abstract a construct containing a human lactoferrin cdna was used to transform tobacco (nicotiana tabacum) using an agrobacterium-mediated dna-transfer system to express this human protein in transgenic plants. transformants were analyzed by southern, northern, and western blots to determine integration of the cdna into the plant genome and lactoferrin gene expression levels. most transgenic plants demonstrated significant delays of bacterial wilt symptoms when inoculated with the bacterial path ... | 1998 | 18944947 |
| effect of a soil amendment on the survival of ralstonia solanacearum in different soils. | abstract the effect of a soil amendment (sa) composed of urea (200 kg of n per ha) and cao (5,000 kg/ha) on the survival of ralstonia solanacearum in four philippine soils was investigated in a series of laboratory experiments. within 3 weeks after application, the sa either caused an initial decrease, a final decline, or no change in the pathogen population, depending on the particular soil type. an initial decrease occurred in a soil with a basic ph and resulted in a significantly (p < 0.001) ... | 1998 | 18944952 |
| resistance and susceptibility of arabidopsis thaliana to bacterial wilt caused by ralstonia solanacearum. | abstract tomato bacterial wilt caused by ralstonia solanacearum is a model system for studying plant-bacterial interactions, because it is genetically one of the best characterized plant diseases. we demonstrate here that four different strains of r. solanacearum, two from radishes (rd4 and rd15) and two from tomato (ps21 and ps95), can infect 27 different ecotypes of arabidopsis thaliana, causing different responses. all ecotypes tested were highly susceptible to strain rd15, which caused sympt ... | 1998 | 18944956 |
| role of extracellular polysaccharide and endoglucanase in root invasion and colonization of tomato plants by ralstonia solanacearum. | abstract ralstonia solanacearum is a soilborne plant pathogen that normally invades hosts through their roots and then systemically colonizes aerial tissues. previous research using wounded stem infection found that the major factor in causing wilt symptoms was the high-molecular-mass acidic extracellular polysaccharide (eps i), but the beta-1,4-endoglucanase (eg) also contributes to virulence. we investigated the importance of eps i and eg for invasion and colonization of tomato by infesting so ... | 1997 | 18945028 |
| enhanced phytoextraction of an agricultural cr- and pb-contaminated soil by bioaugmentation with siderophore-producing bacteria. | bioaugmentation-assisted phytoextraction may enhance the phytoextraction efficiency thanks to larger metal mobilization by microbial metabolites. green fluorescent protein-tagged cells of pseudomonas aeruginosa, pseudomonas fluorescens or ralstonia metallidurans, able to produce siderophores, were inoculated in an agricultural soil containing cr (488 mg kg(-1)) and pb (382 mg kg(-1)) and maize was cultivated. bacteria were inoculated as free or immobilized cells in ca-alginate beads, with skim m ... | 2009 | 18945474 |
| genomic characterization of ralstonia solanacearum phage phirsb1, a t7-like wide-host-range phage. | phirsb1 is a wide-host-range, t7-like bacteriophage that infects and efficiently lyses the phytopathogenic bacterium ralstonia solanacearum. the phirsb1 genome comprises 43,079 bp of double-stranded dna (61.7% g+c) with 325-bp terminal repeats and contains 47 open reading frames. strong activity of tandem early promoters and wide specificity of phage promoters of phirsb1 were demonstrated. | 2009 | 18952798 |
| purification and gene cloning of alpha-methylserine aldolase from ralstonia sp. strain aj110405 and application of the enzyme in the synthesis of alpha-methyl-l-serine. | by screening microorganisms that are capable of assimilating alpha-methyl-dl-serine, we detected alpha-methylserine aldolase in ralstonia sp. strain aj110405, variovorax paradoxus aj110406, and bosea sp. strain aj110407. a homogeneous form of this enzyme was purified from ralstonia sp. strain aj110405, and the gene encoding the enzyme was cloned and expressed in escherichia coli. the enzyme appeared to be a homodimer consisting of identical subunits, and its molecular mass was found to be 47 kda ... | 2008 | 18952881 |
| lead(ii) resistance in cupriavidus metallidurans ch34: interplay between plasmid and chromosomally-located functions. | proteome and transcriptome analysis, combined with mutagenesis, were used to better understand the response of cupriavidus metallidurans ch34 against lead(ii). structural pb(ii)-resistance genes of the pmol30-encoded pbrutrabcd operon formed the major line of defense against pb(ii). however, several general stress response mechanisms under the control of alternative sigma factors such as sigma24/rpok, sigma32/rpoh and sigma28/flia were also induced. in addition, the expression of the pbrr(2) cad ... | 2009 | 18953667 |
| in vitro activities of a novel nanoemulsion against burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species. | respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (cf). species within the burkholderia cepacia complex are especially problematic in this patient population. we investigated a novel surfactant-stabilized oil-in-water nanoemulsion (nb-401) for activity against 150 bacterial isolates recovered primarily from cf respiratory tract specimens. these specimens includ ... | 2009 | 18955531 |
| survival strategies and pathogenicity of ralstonia solanacearum phylotype ii subjected to prolonged starvation in environmental water microcosms. | survival strategies exhibited over 4 years by ralstonia solanacearum phylotype (ph) ii biovar (bv) 2 in environmental water microcosms were examined. the bacterium is a devastating phytopathogen whose ph ii bv 2 causes bacterial wilt in solanaceous crops and ornamental plants. outbreaks of the disease may originate from dissemination of the pathogen in watercourses, where it has to cope with prolonged nutrient limitation. to ascertain the effect of long-term starvation on survival and pathogenic ... | 2008 | 18957611 |
| genomic view of energy metabolism in ralstonia eutropha h16. | ralstonia eutropha is a strictly respiratory facultative lithoautotrophic beta-proteobacterium. in the absence of organic substrates, h2 and co2 are used as sole sources of energy and carbon. in the absence of oxygen, the organism can respire by denitrification. the recent determination of the complete genome sequence of strain h16 provides the opportunity to reconcile the results of previous physiological and biochemical studies in light of the coding capacity. these analyses revealed genes for ... | 2009 | 18957861 |
| ralstonia eutropha strain h16 as model organism for pha metabolism and for biotechnological production of technically interesting biopolymers. | the gram-negative, facultative chemolithoautotrophic bacterium ralstonia eutropha has been intensively investigated for almost 50 years. today it is the best studied 'knallgas' bacterium and producer of poly(3-hydroxybutyric acid). this polyester provides the basis for renewable resource-based biodegradable plastic materials and has attracted much biotechnological interest. the polymer is accumulated in large amounts in the cell and can be used for various applications ranging from replacement o ... | 2009 | 18957865 |
| the genome organization of ralstonia eutropha strain h16 and related species of the burkholderiaceae. | ralstonia eutropha strain h16 is a facultatively chemolithoautotrophic, hydrogen-oxidizing bacterium belonging to the family burkholderiaceae of the betaproteobacteria. the genome of r. eutropha h16 consists of two chromosomes (chr1, chr2) and one megaplasmid (phg1), and thus shows a multi-replicon architecture, which is characteristic for all members of the burkholderiaceae sequenced so far. the genes for housekeeping cell functions are located on chr1. in contrast, many characteristic traits o ... | 2009 | 18957867 |
| improving the thermostability of n-carbamyl-d-amino acid amidohydrolase by error-prone pcr. | to facilitate the easier production of d-amino acids using n-carbamyl-d-amino acid amidohydrolase (dcase) in an immobilized form, we improved the enzymatic thermostability of highly soluble dcase-m3 of ralstonia pickettii using directed mutagenesis. six novel mutation sites were identified in this study, apart from several thermostability-related amino acid sites reported previously. the most thermostable mutant, in which the 12th amino acid had been changed from glutamine to leucine, showed a 7 ... | 2009 | 18985337 |
| oxygen-tolerant h2 oxidation by membrane-bound [nife] hydrogenases of ralstonia species. coping with low level h2 in air. | knallgas bacteria such as certain ralstonia spp. are able to obtain metabolic energy by oxidizing trace levels of h2 using o2 as the terminal electron acceptor. the [nife] hydrogenases produced by these organisms are unusual in their ability to oxidize h2 in the presence of o2, which is a potent inactivator of most hydrogenases through attack at the active site. to probe the origin of this unusual o2 tolerance, we conducted a study on the membrane-bound hydrogenase from ralstonia eutropha h16 an ... | 2009 | 18990688 |
| cognitive optimization of microbial phb production in an optimally dispersed bioreactor by single and mixed cultures. | cognitive (or intelligent) models are often superior to mechanistic models for nonideal bioreactors. two kinds of cognitive models--cybernetic and neural--were applied recently to fed-batch fermentation by ralstonia eutropha in a bioreactor with optimum finite dispersion. in the present work, these models have been applied in simulation studies of co-cultures of r. eutropha and lactobacillus delbrueckii. the results for both cognitive and mechanistic models have been compared with single culture ... | 2009 | 19005684 |
| concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [nife] hydrogenase. | [nife] hydrogenases catalyze the reversible conversion of h2 into protons and electrons. the reaction takes place at the active site, which is composed of a nickel and an iron atom and three diatomic ligands, two cyanides and one carbon monoxide, bound to the iron. the nife(cn-)2co cofactor is synthesized by an intricate posttranslational maturation process, which is mediated by a set of six conserved hyp proteins. depending on the cellular location and the physiological function, additional aux ... | 2009 | 19017638 |
| distinguishing bacterial pathogens of potato using a genome-wide microarray approach. | a set of 9676 probes was designed for the most harmful bacterial pathogens of potato and tested in a microarray format. gene-specific probes could be designed for all genes of pectobacterium atrosepticum, c. 50% of the genes of streptomyces scabies and c. 30% of the genes of clavibacter michiganensis ssp. sepedonicus utilizing the whole-genome sequence information available. for streptomyces turgidiscabies, 226 probes were designed according to the sequences of a pathogenicity island containing ... | 2008 | 19018999 |
| production and characterization of poly-(3-hydroxybutyrate) from recombinant escherichia coli grown on cheap renewable carbon substrates. | although the biopolymer poly-(3-hydroxybutyrate), p[3hb], presents physicochemical properties that make it an alternative material to conventional plastics, its biotechnological production is quite expensive. as carbon substrates contribute greatly to p[3hb] production cost, the utilization of a cheaper carbon substrate and less demanding micro-organisms should decrease its cost. in the present study a 2(3) factorial experimental design was applied, aiming to evaluate the effects of using hydrol ... | 2008 | 19039071 |
| benzene degradation by ralstonia pickettii pko1 in the presence of the alternative substrate succinate. | the regulation of benzene degradation by ralstonia pickettii pko1 in the presence of the alternative substrate succinate was investigated in batch and continuous culture. in batch culture, r. pickettii pko1 achieved a maximum specific growth rate with benzene of 0.18 h(-1), while succinate allowed much faster growth (mu(max) = 0.5 h(-1)). under carbon excess conditions succinate repressed benzene consumption resulting in diauxic growth whereas under carbon-limited conditions in the chemostat bot ... | 2009 | 19039669 |
| interfacial degradation of biodegradable polyester monolayers at the air/enzyme-containing water interface. | the initial enzymatic degradation behavior of langmuir monolayer films of a series of biodegradable polyesters at a constant surface pressure was investigated at the air/water interface. the initial degradation of polyester monolayers strongly depended on the structural formula of the polyesters. by the co-polymerization of 3-hydroxyvalerate (3hv) into the poly[(r)-3-hydroxybutyrate] backbone, the critical surface pressure at which the degradation occurs was increased and the rate of enzymatic d ... | 2008 | 19049118 |
| metabolic engineering of saccharomyces cerevisiae for the production of n-butanol. | abstract: background: increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol. results and conclusion: saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (s. cerevisiae, escherichia coli, clostridium beijerinckii, and ralstonia eutropha) were substituted for the clostridial enzymes and their ... | 2008 | 19055772 |
| host recognition and integration of filamentous phage phirsm in the phytopathogen, ralstonia solanacearum. | two prophages, called varphirsm3 and varphirsm4, that are closely related to, but differ from, filamentous phage varphirsm1, have been detected in strains of the ralstonia solanacearum species complex. the prophage varphirsm3, found in host strain maff730139, could be converted to infectious phage by means of pcr and transfection. the nucleotide sequence of varphirsm3 is highly conserved relative to varphirsm1 except for open reading frame 2 (orf2), encoding an unknown protein, and orf9 encoding ... | 2009 | 19059619 |
| the global virulence regulator phca negatively controls the ralstonia solanacearum hrp regulatory cascade by repressing expression of the prhir signaling proteins. | phca positively and negatively regulates many genes responsible for pathogenicity of ralstonia solanacearum. the type iii secretion system-encoding hrp regulon is one of the negatively controlled operons. phca bound to the promoter region of prhir and repressed its expression, demonstrating that phca shuts down the most upstream component of a signal transfer system for hrpb activation. | 2009 | 19060134 |
| monitoring catalysis of the membrane-bound hydrogenase from ralstonia eutropha h16 by surface-enhanced ir absorption spectroscopy. | | 2009 | 19067445 |
| isolation and identification of atrazine-degrading bacteria from corn field soil in fars province of iran. | in this study several agricultural fields with a long history of atrazine application in fars province of iran have been explored for their potential of atrazine biodegradation. after several subculturing for a period of 300 days acclimation, leads to an enhancement of atrazine biodegradation rate. a successful enrichment culture with a high capability for atrazine degradation was obtained (88%). a combination of enrichment culture technique, in a basal salt medium containing atrazine and carbon ... | 2007 | 19069990 |
| the lcb2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and bax-induced cell death. | previous results showed that expression of the gene encoding the long-chain base2 (lcb(2)) subunit of serine palmitoyltransferase (spt), designated bclcb(2), from nonheading chinese cabbage (brassica campestris ssp. chinensis) was up-regulated during hypersensitive cell death (hcd) induced by the phytophthora boehmeriae elicitor pb90. overexpression of bclcb(2) in nicotiana tabacum leaves suppressed the hcd normally initiated by elicitors and pb90-triggered h(2)o(2) accumulation. bclcb(2) also f ... | 2009 | 19076721 |
| host and non-host plant response to bacterial wilt in potato: role of the lipopolysaccharide isolated from ralstonia solanacearum and molecular analysis of plant-pathogen interaction. | ralstonia solanacearum is one of the most devastating phytopathogenic bacteria, in particular its race 3. this microorganism is the causal agent of destructive diseases of different crops including tomato and potato. an important aspect of the interaction between this pathogen, and the host and non-host plants was its biochemical and molecular basis. thus, the lipopolysaccharides (lps) were extracted from the r. solanacearum cell wall, purified, and the o-specific polysaccharide (ops) was isolat ... | 2008 | 19089824 |
| effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (phb) in recombinant escherichia coli. | nine anaerobic promoters were cloned and constructed upstream of phb synthesis genes phbcab from ralstonia eutropha for the micro- or anaerobic phb production in recombinant escherichia coli. among the promoters, the one for alcohol dehydrogenase (padhe) was found most effective. recombinant e. coli jm 109 (pwcy09) harboring padhe and phbcab achieved a 48% phb accumulation in the cell dry weight after 48 h of static culture compared with only 30% phb production under its native promoter. sixty-s ... | 2009 | 19107470 |
| detection of ralstonia solanacearum in natural substrates using phage amplification integrated with real-time pcr assay. | a sensitive, selective, and rapid protocol for detecting ralstonia solanacearum from soil and plant tissues was developed based on the integration of the rapid self-replicating ability of bacteriophages with quantitative pcr (q-pcr). six bacteriophages were isolated and selected for their ability to specifically infect and lyse r. solanacearum. sixty-three strains of r. solanacearum and 72 isolates of other bacterial species were tested for their susceptibility to the bacteriophages. based on th ... | 2009 | 19138710 |
| degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method. | nonylphenol ethoxylate (npeo)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. eight npeo-degrading strains distributed in genera pseudomonas, sphingomonas, sphingobium, cupriavidus, ralstonia, achromobacter, and staphylococcus were acquired. the latter five genera have never been reported for the degradation of npeos. four degradation patterns were observed for the eight pure strains. in pa ... | 2008 | 19143306 |
| secreted proteins from ralstonia solanacearum: a hundred tricks to kill a plant. | sequence analysis of bacterial genomes has revealed a tremendous potential for protein secretion. this is certainly true for the plant pathogen ralstonia solanacearum which is estimated to export hundreds of proteins through several specialized protein secretion systems. central to pathogenicity are the type ii and type iii secretion systems both of which serve to export large repertoires of pathogenicity effectors. the distribution and the conservation of type iii effectors into bacterial popul ... | 2009 | 19144559 |
| natural products from environmental dna hosted in ralstonia metallidurans. | metagenomic studies designed to access new small molecules from the heterologous expression of environmental dna have focused on the use of two model systems, escherichia coli and streptomyces spp., as heterologous hosts. accessing the biosynthetic potential of dna extracted from the bacteria present in environmental samples will require the development of a more diverse collection of model bacterial hosts that can be used for screening environmental dna libraries. in this study the bacterium ra ... | 2009 | 19146479 |
| derivatization of bioactive carbazoles by the biphenyl-degrading bacterium ralstonia sp. strain sbug 290. | different 9h-carbazole derivatives have been investigated within the last decades due to their broad range of pharmacological applications. while the metabolism of 9h-carbazole has previously been reported, nothing was known about the bacterial transformation of 2,3,4,9-tetrahydro-1h-carbazole and 9-methyl-9h-carbazole. thus, for the first time, the bacterial biotransformation of 2,3,4,9-tetrahydro-1h-carbazole and 9-methyl-9h-carbazole was analyzed using biphenyl-grown cells of ralstonia sp. st ... | 2009 | 19148631 |
| distribution and diversity of autotrophic bacteria in groundwater systems based on the analysis of rubisco genotypes. | a molecular approach, based on the detection of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) large subunit genes, was applied to investigate the distribution and diversity of autotrophic bacteria in groundwater systems. dna extracts from 48 sampling stations, including a variety of pristine and polluted, shallow and deep-subsurface groundwater samples obtained from germany and austria, served as a template for the pcr amplification of form i (cbbl) and form ii (cbbm) large subunit r ... | 2009 | 19157743 |
| the potato stltpa7 gene displays a complex ca-associated pattern of expression during the early stage of potato-ralstonia solanacearum interaction. | although nonspecific lipid transfer proteins (nsltps) are widely expressed during plant defence responses to pathogens, their functions and regulation are not fully understood. in this article, we report the isolation of a cdna for the new nsltp, stltpa7, from cultivated potato (solanum tuberosum) infected with ralstonia solanacearum. the cdna was predicted to encode a type 1 nsltp containing an n-terminal signal sequence and possessing the characteristic features of nsltps. a phylogenetic analy ... | 2009 | 19161349 |
| how xanthomonas type iii effectors manipulate the host plant. | pathogenicity of xanthomonas and most other gram-negative phytopathogenic bacteria depends on a conserved type iii secretion (t3s) system which injects more than 25 different effector proteins into the plant cell. extensive studies in the last years on the molecular mechanisms of type iii effector function revealed that effector proteins with enzymatic functions seem to play important roles in the interaction of xanthomonas with its host plants, for example, the sumo protease xopd. in addition, ... | 2009 | 19168386 |
| ralstonia pickettii septicemia in a dog with immune-mediated thrombocytopenia. | | 2009 | 19175738 |
| synthesis and antimicrobial activity of 1-benzhydryl-sulfonyl-4-(3-(piperidin-4-yl) propyl)piperidine derivatives against pathogens of lycopersicon esculentum: a structure-activity evaluation study. | several 1-benzhydryl-sulfonyl-4-(3-(piperidin-4-yl)propyl)piperidine derivatives 8(a-j) were prepared by the treatment of substituted benzhydryl chlorides with 4-(3-(piperidin-4-yl)propyl)piperidine followed by n-sulfonation with sulfonyl chlorides in the presence of dry methylene dichloride and triethyl amine. the synthesized compounds were characterized by (1)h-nmr, ir, and elemental analysis. all the synthesized compounds were evaluated in vitro for their efficacy as antimicrobial agents by a ... | 2009 | 19183874 |