| factors associated with mortality in patients with bloodstream infection and pneumonia due to stenotrophomonas maltophilia. | severe infections caused by stenotrophomonas maltophilia are associated with high mortality, and strategies to improve the clinical outcome for infected patients are needed. a retrospective cohort study of patients with bloodstream infection (bsis) and pneumonia caused by s. maltophilia was conducted. multivariate analysis was performed to access factors associated with 14-day mortality. a total of 60 infections were identified. among these, eight (13%) were pneumonias and 52 were bsis; 33.3% we ... | 2008 | 18483755 |
| stenotrophomonas maltophilia prosthetic valve endocarditis: a case report. | stenotrophomonas maltophilia is an environmental bacterium increasingly involved in nosocomial infections and resistant to most antibiotics. it is important to recognize and efficiently treat infections with this bacterium as soon as possible. | 2008 | 18500979 |
| stenotrophomonas maltophilia bacteraemia in turkish children. | stenotrophomonas maltophilia is an important cause of life-threatening nosocomial infection. | 2008 | 18510823 |
| antimicrobial susceptibility of 3931 organisms isolated from intensive care units in canada: canadian national intensive care unit study, 2005/2006. | we tested the in vitro activity of 15 antimicrobials against gram-positive cocci and 12 antimicrobials against gram-negative bacilli versus 3931 isolates (20 most common organisms) obtained between september 1, 2005, and june 30, 2006, from 19 intensive care units (icus) across canada. the most active (based upon mic only) agents against methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus epidermidis were dalbavancin, daptomycin, linezolid, tigecycline, and vanco ... | 2008 | 18513913 |
| biodegradation of 2,4,6-trichlorophenol in a packed-bed biofilm reactor equipped with an internal net draft tube riser for aeration and liquid circulation. | for the aerobic biodegradation of the fungicide and defoliant 2,4,6-trichlorophenol (2,4,6-tcp), a bench-scale packed-bed bioreactor equipped with a net draft tube riser for liquid circulation and oxygenation (pb-alr) was constructed. to obtain a high packed-bed volume relative to the whole bioreactor volume, a high a(d)/a(r) ratio was used. reactor's downcomer was packed with a porous support of volcanic stone fragments. pb-alr hydrodynamics and oxygen mass transfer behavior was evaluated and c ... | 2009 | 18539387 |
| [stenotrophomonas maltophilia bacteremia after endoscopic retrograde cholangiopancreatography]. | | 2008 | 18547765 |
| fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients. | the accurate and rapid identification of bacteria isolated from the respiratory tract of patients with cystic fibrosis (cf) is critical in epidemiological studies, during intrahospital outbreaks, for patient treatment, and for determination of therapeutic options. while the most common organisms isolated from sputum samples are pseudomonas aeruginosa, staphylococcus aureus, and haemophilus influenzae, in recent decades an increasing fraction of cf patients has been colonized by other nonfermenti ... | 2008 | 18550747 |
| the emergence of stenotrophomonas maltophilia. | | 2008 | 18556278 |
| prosthetic aortic valve endocarditis due to stenotrophomonas maltophilia complicated by subannular abscess. | annular abscesses are serious complications of infectious native and prosthetic valve endocarditis. in this patient, we isolated stenotrophomonas maltophilia, a rare cause of subaortic abscess with high mortality/morbidity rates although virulent gram-positive cocci, s. aureus in particular, have been the most commonly isolated agents. we treated this case of endocarditis and the subannular abscess observed 1 year after the initial operation by aortic root replacement with resternotomy in additi ... | 2008 | 18557156 |
| evaluation of a modified selective differential medium for the isolation of stenotrophomonas maltophilia. | via medium for stenotrophomonas maltophilia was modified by substituting meropenem (16 mg/l) for imipenem. s. maltophilia grew from 40% of drains sampled within a hospital and surrounding locations in perth, western australia. the specificity of the new medium for s. maltophilia was 62%, and all contaminating bacteria were easily distinguishable by phenotypic tests and pcr. | 2008 | 18558447 |
| stenotrophomonas maltophilia bacteremia after living donor liver transplantation: report of a case. | stenotrophomonas maltophilia (previously named xanthomonas maltophilia) is an aerobic, nonfermentive, gram-negative bacillus that is widespread in the environment. it is considered to be an organism with a limited pathogenic potential, which is rarely capable of causing diseases in humans other than in those who are in an immunocompromised state. in this study, we outline the case of a patient with stenotrophomonas maltophilia bacteremia after living donor liver transplantation, which showed the ... | 2008 | 18560974 |
| a meningitis case due to stenotrophomonas maltophilia and review of the literature. | stenotrophomonas maltophilia (formerly xanthomonas maltophilia) is a gram-negative bacillus increasingly associated with serious nosocomial infections. here, we describe a 30-year-old male patient who developed meningitis associated with this organism after several neurosurgical procedures. a review of the literature revealed only 15 previous reports. most cases were associated with neurosurgical procedures. antimicrobial therapy is complicated by multiple drug resistance of the organism, and tr ... | 2008 | 18579427 |
| the plant-associated bacterium stenotrophomonas rhizophila expresses a new enzyme for the synthesis of the compatible solute glucosylglycerol. | the rhizobacterium stenotrophomonas rhizophila accumulates the compatible solutes glucosylglycerol (gg) and trehalose under salt stress conditions. the complete gene for the gg synthesis enzyme was cloned and sequenced. this enzyme from s. rhizophila represented a novel fusion protein composed of a putative c-terminal gg-phosphate synthase domain and an n-terminal putative gg-phosphate phosphatase domain, which was named ggpps. a similar gene was cloned from pseudomonas sp. strain oa146. the ggp ... | 2008 | 18586931 |
| induction potential of clavulanic acid toward l1 and l2 beta-lactamases of stenotrophomonas maltophilia. | | 2008 | 18592278 |
| microbial diversity of soil bacteria in agricultural field contaminated with heavy metals. | in this study we evaluated the bacterial diversity in a soil sample from a site next to a chemical industrial factory previously contaminated with heavy metals. analysis of 16s rdna sequences amplified from dna directly extracted from the soil revealed 17 different bacterial types (genera and/or species). they included polyangium spp., sphingomonas spp., variovorax spp., hafina spp., clostridia, acidobacteria, the enterics and some uncultured strains. microbes able to tolerate high concentration ... | 2008 | 18595405 |
| identification and management of unusual pathogens in cystic fibrosis. | | 2008 | 18607011 |
| comparative in vitro activity of l-ofloxacin and fk037 to other agents against 10,040 fresh clinical isolates. | fresh clinical isolates collected from november 1, 1992 through november 1, 1993, were tested by agar dilution against 26 different antimicrobial agents including fk037 and l-ofloxacin. among the 10 040 organisms tested were staphylococcus aureus (n = 1222), methicillin-resistant staphylococcus aureus (mrsa, n = 455), staphylococcus epidermidis (n = 533), staphylococcus hominis (n = 90), staphylococcus hemolyticus (n = 89), streptococcus pneumoniae (n = 144), escherichia coli (n = 2326), klebsie ... | 1996 | 18611710 |
| polymicrobial bacteremia in cancer patients: analysis of risk factors, etiology and outcome in 214 episodes. | two hundred and fourteen episodes of polymicrobial bacteremia in 182 cancer patients in a period of 6 years in a 360-bed national cancer institute were analyzed for etiology, risk factors and outcome. variables were compared with 187 episodes of monomicrobial bacteremias in 147 cancer patients to find statistical significance among risk factors, etiology and outcome. urinary catheters and breakthrough bacteremia were the only risk factors associated with polymicrobial in comparison to monomicrob ... | 1996 | 18611744 |
| oil-removal enhancement in media with keratinous or chitinous wastes by hydrocarbon-degrading bacteria isolated from oil-polluted soils. | the aim of this work was to isolate oil-degrading bacteria that use chitin or keratin as carbon sources from oil contaminated soils; and additionally to study if oil removal by these bacteria is enhanced when a chitinous or a keratinous waste is added to the culture media. to isolate the above-mentioned bacteria, 12 soil samples were collected close to an oil-well. such soils showed unsuitable nutrients content, but their counts of heterotrophic bacteria ranged within 10(5)-10(8) cfu g(-1) soil, ... | 2008 | 18613616 |
| risk factors associated with mortality of infections caused by stenotrophomonas maltophilia: a systematic review. | the objective of this review was to assess the quality of available literature regarding risk factors associated with mortality of infections caused by s. maltophilia. pubmed and ovid were searched from march 1985 to march 2008, and eligible studies were considered to be those that related s. maltophilia infection with risk factors associated with mortality; described the characteristics of patients in detail; and provided data regarding the outcome and mortality. thirty-eight studies were found ... | 2008 | 18621440 |
| evidence for specificity of cultivable bacteria associated with arbuscular mycorrhizal fungal spores. | bacteria associated with arbuscular mycorrhizal (am) fungal spores may play functional roles in interactions between am fungi, plant hosts and defence against plant pathogens. to study am fungal spore-associated bacteria (amb) with regard to diversity, source effects (am fungal species, plant host) and antagonistic properties, we isolated amb from surface-decontaminated spores of glomus intraradices and glomus mosseae extracted from field rhizospheres of festuca ovina and leucanthemum vulgare. a ... | 2008 | 18631178 |
| smqnr, a new chromosome-carried quinolone resistance gene in stenotrophomonas maltophilia. | a new chromosome-carried quinolone resistance gene from stenotrophomonas maltophilia, smqnr, was characterized. the gene was present in type strain ccug 5866 and was also detected in 24 clinical isolates and showed some allelic diversity. the expression of smqnr in escherichia coli decreased the susceptibilities of the e. coli isolates to several fluoroquinolones. | 2008 | 18644963 |
| culturable aerobic bacteria from the upstream region of a karst water rivulet. | the composition of 681 aerobic and heterotrophic strains that were isolated on two different media was assessed at four sampling points along a approximately 300 m stretch of a karst water rivulet. based on partial sequence analysis of 16s rrna genes, members of 35 genera were identified; however, only a few species dominated as their representatives were repeatedly isolated at different sampling sites. determination of the phylum affiliation showed that the isolates included members of bacterio ... | 2008 | 18645959 |
| anti-adherence activity and antimicrobial durability of anti-infective-coated catheters against multidrug-resistant bacteria. | to investigate the anti-adherence and antimicrobial durability of anti-infective catheters against multidrug-resistant (mdr) staphylococcus aureus (resistant to vancomycin, rifampicin and methicillin) and mdr gram-negative bacteria (stenotrophomonas maltophilia, acinetobacter baumannii/calcoaceticus and enterobacter agglomerans) that are often associated with catheter-related bloodstream infections (crbsis). | 2008 | 18653489 |
| therapeutic options for stenotrophomonas maltophilia infections beyond co-trimoxazole: a systematic review. | stenotrophomonas maltophilia has emerged as an important opportunistic pathogen, causing infections whose management is often problematic due to its inherent resistance to many antibiotics, making co-trimoxazole the main therapeutic option. however, there are cases in which either due to antimicrobial resistance or allergic reactions and intolerance to co-trimoxazole this antibiotic cannot be administered. we sought to evaluate the available clinical evidence regarding potentially effective alte ... | 2008 | 18662945 |
| polymerase chain reaction detection of bacterial 16s rrna gene in human blood. | bacterial 16s ribosomal rna genes (rdna) were detected in blood samples from two healthy individuals by pcr under conditions involving 30 cycles that did not produce any visible products from negative control saline. even from control samples, pcr involving 35-40 cycles yielded visible bands. major clones detected in the blood samples, but not in control, were the aquabacterium subgroup, stenotrophomonas subgroup, budvicia subgroup, serratia subgroup, bacillus subgroup and flavobacteria subgroup ... | 2008 | 18667036 |
| prevalence of antibiotic-resistant bacteria in herbal products. | the objective of this study was to determine the prevalence of antibiotic-resistant bacteria in various herbal products. twenty-nine herbal supplements (18 traditional and 11 organic products) were purchased from stores and analyzed microbiologically. total bacterial counts were determined by pour plate and surface spreading on tryptic soy agar (tsa). antibiotic-resistant bacteria were enumerated on tsa supplemented with ceftriaxone (64 microg/ml) or tetracycline (16 microg/ml). total bacterial ... | 2008 | 18680952 |
| factors associated with adherence to and biofilm formation on polystyrene by stenotrophomonas maltophilia: the role of cell surface hydrophobicity and motility. | we tested 40 clinical stenotrophomonas maltophilia strains to investigate the possible correlation between adherence to and formation of biofilm on polystyrene, and cell surface properties such as hydrophobicity and motility. most of the strains were able to adhere and form biofilm, although striking differences were observed. eleven (27.5%) of the strains were hydrophobic, with hydrophobicity greatly increasing as s. maltophilia attached to the substratum. a positive correlation was observed be ... | 2008 | 18681866 |
| predictors of 30-day mortality and hospital costs in patients with ventilator-associated pneumonia attributed to potentially antibiotic-resistant gram-negative bacteria. | to identify predictors of 30-day mortality and hospital costs in patients with ventilator-associated pneumonia (vap) attributed to potentially antibiotic-resistant gram-negative bacteria (pargnb) [pseudomonas aeruginosa, acinetobacter species, and stenotrophomonas maltophilia]. | 2008 | 18682456 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| hospital acquired pneumonia with high-risk bacteria is associated with increased pulmonary matrix metalloproteinase activity. | neutrophil products like matrix metalloproteinases (mmp), involved in bacterial defence mechanisms, possibly induce lung damage and are elevated locally during hospital- acquired pneumonia (hap). in hap the virulence of bacterial species is known to be different. the aim of this study was to investigate the influence of high-risk bacteria like s. aureus and pseudomonas species on pulmonary mmp concentration in human pneumonia. | 2008 | 18700005 |
| ventilator-associated pneumonia caused by high risk microorganisms: a matched case-control study. | to determine the impact of ventilator-associated pneumonia (vap) caused by high risk microorganisms (hrm) on patient outcome. design: matched case-control study. the study was conducted in a medical intensive care unit (icu) of a university hospital. thirty-five patients with vap caused by hrm, including pseudomonas aeruginosa, acinetobacter spp., stenotrophomonas maltophilia and/or methicillin-resistant staphylococcus aureus were accepted as the case the patients. thirty-five control patients, ... | 2008 | 18701973 |
| risk factors for mortality and clinical implications of catheter-related infections in patients with bacteraemia caused by stenotrophomonas maltophilia. | | 2008 | 18715768 |
| emerging pathogens in cystic fibrosis: ten years of follow-up in a cohort of patients. | in patients with cystic fibrosis (cf), there is an increasing incidence of some uncommon respiratory pathogens, such as burkholderia cepacia complex (bcc), stenotrophomonas maltophilia, and achromobacter xylosoxidans. in order to evaluate the prevalence and the clinical impact of these pathogens, we retrospectively studied a total of 109 patients followed in our center from 1996 to 2006 and reviewed the results of 1,550 sputum samples. the isolation of pseudomonas aeruginosa slightly decreased o ... | 2009 | 18758832 |
| diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots. | forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ... | 2008 | 18759227 |
| pseudoxanthomonas sacheonensis sp. nov., isolated from btex-contaminated soil in korea, transfer of stenotrophomonas dokdonensis yoon et al. 2006 to the genus pseudoxanthomonas as pseudoxanthomonas dokdonensis comb. nov. and emended description of the genus pseudoxanthomonas. | a gram-negative, strictly aerobic, rod-shaped bacterium, designated strain bd-c54(t), was isolated from btex (benzene, toluene, ethylbenzene and xylenes)-contaminated soil in sacheon, korea. growth of strain bd-c54(t) was observed at 15-35 degrees c (optimum 25-30 degrees c) and ph 6.0-9.5 (optimum ph 7.0-8.0). the predominant fatty acids were iso-c(15:0), iso-c(17:1)omega9c, iso-c(11:0) 3-oh, iso-c(16:0), iso-c(11:0) and iso-c(17:0). the strain contained large amounts of phosphatidylethanolamin ... | 2008 | 18768635 |
| effect of bacterial interference on biofilm development by legionella pneumophila. | in the ecology of legionella pneumophila a crucial role may be played by its relationship with the natural flora; thus we investigated the interactions between legionella and other aquatic bacteria, particularly within biofilms. among 80 aquatic bacteria screened for the production of bacteriocin-like substances (blss), 66.2% of them were active against l. pneumophila. the possible effect of some of these aquatic bacteria on the development and stability of l. pneumophila biofilms was studied. p ... | 2008 | 18769851 |
| acquisition and spread of acinetobacter baumannii and stenotrophomonas maltophilia in intensive care patients. | acinetobacter baumannii and stenotrophomonas maltophilia are increasingly important pathogens, especially in the intensive care units (icus). this study was designed to investigate the clonality, the mode of transmission and the patients' risk profile for acquisition of a. baumannii and s. maltophilia at the icu of an italian hospital. patterns of a. baumannii and s. maltophilia acquisition in the icu during the period of the survey were carriage, colonization and infection. characterization of ... | 2009 | 18771951 |
| clinical impact of the over-expression of efflux pump in nonfermentative gram-negative bacilli, development of efflux pump inhibitors. | in this manuscript, we want to review the biochemical and genetic characteristics of the different efflux pumps involved in both intrinsic and acquired multiresistance in non-fermentative gram-negative bacteria such as pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia, as well as the regulation of their expression. moreover, the clinical impact of the over-expression of these efflux pumps and the investigation developed to define efflux pump inhibitors will be dis ... | 2008 | 18781925 |
| chemotaxonomy of bacteria by comprehensive gc and gc-ms in electron impact and chemical ionisation mode. | the analysis of the cellular lipidic fraction of bacteria is described. after hydrolysis and methylation, the fatty acid methyl esters (fames) are determined by 1-d gc using the sherlock midi bacteria identification system, by comprehensive gc (gc x gc) and by gc-ms in electron impact (ei) and positive chemical ionisation (pci) mode. with gc x gc, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. gc-ei-ms and gc-pci-ms w ... | 2008 | 18792008 |
| evolution of antimicrobial susceptibility patterns of aerobic and facultative gram-negative bacilli causing intra-abdominal infections: results from the smart studies 2003-2007. | study for monitoring antimicrobial resistance trends (smart) is an ongoing global antimicrobial surveillance program focused on clinical isolates from intra-abdominal infections (iai). the objective of this subanalysis was to assess the evolution of the antimicrobial susceptibility patterns among aerobic and facultative gram-negative bacilli (gnb) recovered over a 5-year period at our institution. we tested the in vitro activity of the antimicrobials, commonly used to treat iai, against consecut ... | 2008 | 18792817 |
| predictive analysis of transmissible quinolone resistance indicates stenotrophomonas maltophilia as a potential source of a novel family of qnr determinants. | predicting antibiotic resistance before it emerges at clinical settings constitutes a novel approach for preventing and fighting resistance of bacterial pathogens. to analyse the possibility that novel plasmid-encoded quinolone resistance determinants (qnr) can emerge and disseminate among bacterial pathogens, we searched the presence of those elements in nearly 1000 bacterial genomes and metagenomes. | 2008 | 18793450 |
| antimicrobial activities of the essential oils of origanum onites l., origanum vulgare l. subspecies hirtum (link) ietswaart, satureja thymbra l., and thymus cilicicus boiss. & bal. growing wild in turkey. | in the present study, four separate samples of origanum onites l., three separate samples of satureja thymbra l., origanum vulgare l. ssp. hirtum (link) ietswaart, and thymus cilicicus boiss. & bal. were collected from various regions of mugla, turkey. the essential oils of these plants were obtained by the hydrodistillation method. antimicrobial activities of the essential oils against microorganisms, including multiple antibiotic-resistant bacteria, were investigated using the disc diffusion m ... | 2008 | 18800908 |
| antibacterial agents and heavy metal resistance in gram-negative bacteria isolated from seawater, shrimp and sediment in iskenderun bay, turkey. | the aim of the present study was to determine the level of antibiotic resistance patterns and distribution of heavy metal resistance of bacterial isolates from seawater, sediment and shrimps, and to determine if there is a relationship between antibiotic and heavy metal resistance. we undertook studies in 2007 in the industrially polluted iskenderun bay, on the south coast of turkey. the resistance of 236 gram-negative bacterial isolates (49 from seawater, 90 from sediment and 97 from shrimp) to ... | 2008 | 18804847 |
| trends in stenotrophomonas maltophilia bloodstream infection in relation to usage density of cephalosporins and carbapenems during 7 years. | | 2008 | 18808347 |
| fluorescence spectroscopy as a promising tool for a polyphasic approach to pseudomonad taxonomy. | fluorescence spectroscopy is an emerging tool for the analysis of biomolecules from complex matrices. we explored the potentialities of the method for the pseudomonad taxonomic purpose at the genus and species level. emission spectra of three intrinsic fluorophores (namely, nadh, tryptophan, and the complex of aromatic amino acids and nucleic acid) were collected from whole bacterial cells. their comparisons were performed through principal component analysis and factorial discriminant analysis. ... | 2009 | 18815829 |
| application of compochip microarray to investigate the bacterial communities of different composts. | a microarray spotted with 369 different 16s rrna gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. the microarray was tested with pure cultures, and of the 30,258 individual probe-target hybridization reactions performed, there were only 188 false positive (0.62%) and 22 false negative signals (0.07%). labeled target dna was prepared by polymerase chain reaction amplification of 16s rrna genes using a cy5-labeled universa ... | 2009 | 18818861 |
| non-susceptibility trends among pseudomonas aeruginosa and other non-fermentative gram-negative bacteria from bacteraemias in the uk and ireland, 2001-06. | pseudomonas and acinetobacter spp. are important opportunists, notorious for resistance. pseudomonas spp. are collected in the british society for antimicrobial chemotherapy (bsac) bacteraemia surveillance, with acinetobacter spp. and stenotrophomonas maltophilia well represented in the 'other gram-negatives' group. | 2008 | 18819980 |
| dipeptidyl aminopeptidase iv from stenotrophomonas maltophilia exhibits activity against a substrate containing a 4-hydroxyproline residue. | the crystal structure of dipeptidyl aminopeptidase iv from stenotrophomonas maltophilia was determined at 2.8-a resolution by the multiple isomorphous replacement method, using platinum and selenomethionine derivatives. the crystals belong to space group p4(3)2(1)2, with unit cell parameters a = b = 105.9 a and c = 161.9 a. dipeptidyl aminopeptidase iv is a homodimer, and the subunit structure is composed of two domains, namely, n-terminal beta-propeller and c-terminal catalytic domains. at the ... | 2008 | 18820015 |
| cross-reaction of stenotrophomonas and xanthomonas species in a 23s rrna gene-directed pcr for detection of s. maltophilia. | | 2008 | 18842940 |
| [diagnostic and therapeutic management of gram-negative infections]. | among gram negative bacteria, pseudomonas aeruginosa, the extended spectrum beta-lactamases (esbl)-producing strains, acinetobacter spp, in particular the multiresistant acinetobacter baumannii, and stenotrophomonas maltophilia are the most implicated micrororganisms in the ever more increasing problem of bacterial resistance. possible solutions have to be searched, on one hand, in the use of new drugs but, on the other hand, in the re-evaluation of those already available drugs, possibly consid ... | 2008 | 18843221 |
| development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux. | human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. we describe the construction and development of an active surveillance of pathogens (asp) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes. | 2008 | 18844996 |
| [extended-spectrum beta-lactamases-producing stenotrophomonas maltophilia strains: ctx-m enzymes detection and virulence study]. | to study the beta-lactamases content of stenotrophomonas maltophilia strains and to evaluate the virulence potential of these strains with the in vivo caenorhabditis elegans model. | 2008 | 18848407 |
| [oligonucleotide primers for detection and amplification of the emoa gene encoding bacterial ethylenediaminetetraacetate monooxygenase]. | a system of primers was designed on the basis of analysis of nucleotide sequences of the emoa gene encoding ethylenediaminetetraacetate (edta) monooxygenase, which are deposited in genbank. this system of primers makes it possible to obtain emoa gene fragments approximately 750 bp long for bacterial destructors of edta. polymerase chain reaction (pcr) of total dna isolated from enrichment and pure cultures showed that this system can be effectively used for detecting the emoa gene in representat ... | 2008 | 18924405 |
| dgge with genomic dna: suitable for detection of numerically important organisms but not for identification of the most abundant organisms. | identification of all important community members as well as of the numerically dominant members of a community are key aspects of microbial community analysis of bioreactor samples. a systematic study was conducted with artificial consortia to test whether denaturing gradient gel electrophoresis (dgge) is a reliable technique to obtain such community data under conditions where results would not be affected by differences in dna extraction efficiency from cells. a total of 27 consortia were est ... | 2008 | 18929384 |
| risa-hplc analysis of lung bacterial colonizers of cystic fibrosis children. | microbiological analysis of sputum samples, from children affected by cystic fibrosis (cf) and showing signs of acute or chronic infections, is routinely performed by culture-dependent approaches involving selective media and biochemical tests. these identification schemes are time-consuming, and may lead to false negative results. the aim of this work was to evaluate the efficacy of a ribosomal intergenic spacer analysis (risa) coupled to high performance liquid chromatography (hplc) for the de ... | 2009 | 18929602 |
| a new stenotrophomonas maltophilia strain producing laccase. use in decolorization of synthetics dyes. | laccase activity was detected in a soil bacterium stenotrophomonas maltophilia aap56 identified by biochemical and molecular methods. it was produced in cells at the stationary growth phase in luria bertani (lb) medium added by 0.4 mm copper sulfate. the addition of cuso(4) in culture medium improved production of laccase activity. however, one laccase enzyme was detected by native polyacrylamide gel electrophoresis. the enzyme showed syringaldazine (k (m) = 53 microm), 2,2'-azino-bis(3-ethylben ... | 2009 | 18931956 |
| a comprehensive species to strain taxonomic framework for xanthomonas. | abstract a comprehensive classification framework was developed that refines the current xanthomonas classification scheme and provides a detailed assessment of xanthomonas diversity at the species, subspecies, pathovar, and subpathovar levels. polymerase chain reaction (pcr) using primers targeting the conserved repetitive sequences box, enterobacterial repetitive intergenic consensus (eric), and repetitive extragenic palindromic (rep) (rep-pcr) was used to generate genomic fingerprints of 339 ... | 2005 | 18943308 |
| influence of temperature and inoculum density of fusarium oxysporum f. sp. ciceris on suppression of fusarium wilt of chickpea by rhizosphere bacteria. | the effects of temperature and inoculum density of fusarium oxysporum f. sp. ciceris race 5 on suppression of fusarium wilt in chickpea (cicer arietinum) cv. pv 61 by seed and soil treatments with rhizobacteria isolated from the chickpea rhizosphere were studied in a model system. disease development over a range of temperatures (20, 25, and 30 degrees c) and inoculum densities (25 to 1,000 chlamydospores per gram of soil) was described by the gompertz model. the gompertz relative rate of diseas ... | 2001 | 18944039 |
| chitinases from the plant disease biocontrol agent, stenotrophomonas maltophilia c3. | abstract stenotrophomonas maltophilia strain c3, a biocontrol agent of bipolaris sorokiniana in turfgrass, produced chitinases in broth media containing chitin. chitinases were partially purified from culture fluid by ammonium sulfate precipitation and chitin affinity chromatography. the chromatography fraction with the highest specific chitinase activity was inhibitory to conidial germination and germ-tube elongation of b. sorokiniana, but it was less inhibitory than the protein fraction or the ... | 2001 | 18944395 |
| the role of chitinase production by stenotrophomonas maltophilia strain c3 in biological control of bipolaris sorokiniana. | abstract the role of chitinase production by stenotrophomonas maltophilia strain c3 in biological control of leaf spot on tall fescue (festuca arundinacea), caused by bipolaris sorokiniana, was investigated in vitro and in vivo. the filtrate of a broth culture of c3, with chitin as the carbon source, was separated into fractions. a high molecular-weight fraction (>8 kda) was chitinolytic and more inhibitory than a low-molecular-weight, nonchitinolytic fraction to conidial germination and hyphal ... | 2000 | 18944588 |
| biological control of bipolaris sorokiniana on tall fescue by stenotrophomonas maltophilia strain c3. | abstract stenotrophomonas maltophilia strain c3 was evaluated for control of leaf spot on tall fescue (festuca arundinacea) caused by bipolaris sorokiniana. in growth chamber experiments, c3 inhibited conidial germination on leaf surfaces and reduced lesion frequency and percent diseased leaf area compared with nontreated controls. the amount of leaf spot suppression was related to the c3 dose applied. the highest dose tested, 10(9) cfu/ml, prevented nearly all b. sorokiniana conidia from germin ... | 1999 | 18944711 |
| [stenotrophomonas maltophilia and cancer]. | | 2008 | 18949155 |
| isolation of new stenotrophomonas bacteriophages and genomic characterization of temperate phage s1. | twenty-two phages that infect stenotrophomonas species were isolated through sewage enrichment and prophage induction. of them, s1, s3, and s4 were selected due to their wide host ranges compared to those of the other phages. s1 and s4 are temperate siphoviruses, while s3 is a virulent myovirus. the genomes of s3 and s4, about 33 and 200 kb, were resistant to restriction digestion. the lytic cycles lasted 30 min for s3 and about 75 min for s1 and s4. the burst size for s3 was 100 virions/cell, w ... | 2008 | 18952876 |
| in vitro activities of a novel nanoemulsion against burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species. | respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (cf). species within the burkholderia cepacia complex are especially problematic in this patient population. we investigated a novel surfactant-stabilized oil-in-water nanoemulsion (nb-401) for activity against 150 bacterial isolates recovered primarily from cf respiratory tract specimens. these specimens includ ... | 2009 | 18955531 |
| sec- and tat-dependent translocation of beta-lactamases across the escherichia coli inner membrane. | beta-lactamases represent the major resistance mechanism of gram-negative bacteria against beta-lactam antibiotics. the amino acid sequences of these proteins vary widely, but all are located in the periplasm of bacteria. in this study, we investigated the translocation mechanism of representative beta-lactamases in an escherichia coli model. n-terminal signal sequence analyses, antibiotic activity assay, and direct measurement of translocation of a green fluorescent protein (gfp) reporter fused ... | 2009 | 18981261 |
| cluster of stenotrophomonas maltophilia endocarditis after prosthetic valve replacement. | early postoperative prosthetic valve endocarditis due to stenotrophomonas maltophilia was diagnosed in seven patients (two men) aged from 68 to 84 years (mean age 78.1 years) over a three-year period. all patients had undergone aortic valve replacement. s. maltophilia was isolated from at least two blood cultures per patient. four patients experienced cns embolic complications. three patients died. all patients were treated with ceftazidime, one in combination with amikacin, one with ciprofloxac ... | 2008 | 18988010 |
| role of a moderately halophilic bacterial consortium in the biodegradation of polyaromatic hydrocarbons. | polycyclic aromatic hydrocarbons are ubiquitous pollutants in the environment, and most high molecular weight pahs cause mutagenic, teratogenic and potentially carcinogenic effects. while several strains have been identified that degrade pahs, the present study is focused on the degradation of pahs in a marine environment by a moderately halophilic bacterial consortium. the bacterial consortium was isolated from a mixture of marine water samples collected from seven different sites in chennai, i ... | 2009 | 18995870 |
| identification of novel denitrifying bacteria stenotrophomonas sp. zz15 and oceanimonas sp. yc13 and application for removal of nitrate from industrial wastewater. | two novel denitrifying bacteria were successfully isolated from industrial wastewater and soil samples. using morphological, biochemical/biophysical and 16s rrna gene analyses, these two bacteria were identified as stenotrophomonas sp. zz15 and oceanimonas sp. yc13, respectively. both of these two bacteria showed efficient no(3) (-)-n removing abilities under a semi-anaerobic condition without obvious accumulation of no(2) (-)-n, n(2)o-n and nh(4) (+)-n. no(3) (-)-n removal from paper mill waste ... | 2009 | 19002594 |
| growing old with cystic fibrosis - the characteristics of long-term survivors of cystic fibrosis. | the proportion of patients with cystic fibrosis (cf) who are middle-aged is increasing - and is likely to continue to do so. we surveyed a population of long-term cf survivors to assess their burden of illness and profile their disease characteristics. | 2009 | 19022643 |
| [nationwide susceptibility surveillance of ciprofloxacin and various parenteral antimicrobials against bacteria isolated from patients with severe infections--third ciproxan injection special survey (2005)]. | we conducted 3 nationwide surveillance studies between 2001 and 2005 at 39 participating institutions throughout japan according to the special survey plan to investigate susceptibility to ciprofloxacin (cpfx) and various parenteral antimicrobials using clinical isolates from patients with severe infection during the reexamination period of parenteral cpfx. results of the first special survey (2001) were already reported in this journal. the current third special survey (2005) was conducted at 3 ... | 2008 | 19024645 |
| pcr-based rapid genotyping of stenotrophomonas maltophilia isolates. | all bacterial genomes contain repetitive sequences which are members of specific dna families. such repeats may occur as single units, or found clustered in multiple copies in a head-to-tail configuration at specific loci. the number of clustered units per locus is a strain-defining parameter. assessing the length variability of clusters of repeats is a versatile typing methodology known as multilocus variable number of tandem repeat analysis (mlva). | 2008 | 19025624 |
| independent predictors for mortality in patients with positive stenotrophomonas maltophilia cultures. | stenotrophomonas maltophilia is an emerging pathogen in nosocomial infections that may result in high mortality. s. maltophilia often present as part of a polymicrobial culture and it is not well established when treatment is indicated. we aimed to identify predictors of mortality in patients with positive cultures of s. maltophilia. | 2008 | 19037515 |
| viable ultramicrocells in drinking water. | to examine the diversity of cultivable 0.2 micron filtrate biofilm forming bacteria from drinking water systems. | 2009 | 19040704 |
| acquisition and evolution of plant pathogenesis-associated gene clusters and candidate determinants of tissue-specificity in xanthomonas. | xanthomonas is a large genus of plant-associated and plant-pathogenic bacteria. collectively, members cause diseases on over 392 plant species. individually, they exhibit marked host- and tissue-specificity. the determinants of this specificity are unknown. | 2008 | 19043590 |
| purification and characterization of cytoplasmic nad-dependent polypropylene glycol dehydrogenase from stenotrophomonas maltophilia. | the oxidizing enzyme nad(+)-dependent polypropylene glycol dehydrogenase (ppg-dh) was purified to homogeneity from the cytoplasmic fraction of stenotrophomonas maltophilia grown on polypropylene glycol (diol type) 2000. the purified enzyme consisted of a homotetrameric protein (37 kda subunit) with a molecular mass of around 154 kda. the n-terminal amino acid sequence (25 residues) showed similarity to the sequences of nad(+)-dependent secondary alcohol dehydrogenases and nadh-dependent reductas ... | 2008 | 19054086 |
| cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by diamondback moth plutella xylostella (lepidoptera: plutellidae) gut bacteria. | siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (omrps) by diamondback moth (dbm) gut bacterial strains, were all examined. all the tested strains grew in the presence of 2, 2'-dipyridyl, and the brachybacterium sp. psgb10, pseudomonas sp. prgb06, and serratia marcescens flgb16 strains were found to cross-utilize the siderophores of various entomopathogens, including bacillus thuringiensis. ... | 2008 | 19054090 |
| genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees. | the association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. members of the gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. as ... | 2009 | 19060168 |
| study on the bacterial midgut microbiota associated to different brazilian populations of lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae). | the bacterial community associated with the midgut of three brazilian lutzomyia longipalpis (lutz & neiva) populations, two from endemic areas for visceral leishmaniasis (jacobina, bahia state and são luís, maranhão state) and one from a non-endemic area (lapinha cave, minas gerais state), was identified. five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. the species identification was based on molecular and traditional ba ... | 2008 | 19061048 |
| stenotrophomonas maltophilia infections of intact skin: a systematic review of the literature. | to highlight the importance of intact skin infection syndromes caused by stenotrophomonas maltophilia, we review 17 reported cases. skin infection syndrome presentations included metastatic cellulitis (58%), primary cellulitis (23%), and ecthyma gangrenosum (17%). associated risk factors were hematologic malignancies and chemotherapy (94%), neutropenia (94%), presence of central venous catheter (17%), and exposure to broad-spectrum antibiotics (84%). the diagnosis was supported by cultures of sk ... | 2009 | 19070451 |
| the role of ampr in regulation of l1 and l2 beta-lactamases in stenotrophomonas maltophilia. | stenotrophomonas maltophilia is known to produce at least two chromosomal-mediated inducible beta-lactamases, l1 and l2. gene l2, which encodes a class a beta-lactamase, and the adjacent ampr gene form an ampr-class a beta-lactamase module. l1 belongs to the class b beta-lactamase and has no neighbor ampr-like regulatory gene. in this study, the ampr-l2 module from s. maltophilia kh was compared with ampr-beta-lactamase modules from several microorganisms with respect to the ampr and beta-lactam ... | 2009 | 19071216 |
| lung infections after cancer chemotherapy. | lung infections can be severe consequences of chemotherapy-induced immune defects. aetiological causes of infection include bacteria (most commonly pseudomonas aeruginosa, stenotrophomonas maltophilia, and nocardia species), viruses (eg, respiratory syncytial virus, parainfluenza virus, influenza virus a and b, and cytomegalovirus), and fungi (eg, aspergillus, fusarium, and mucorales species, and pneumocystis jirovecii). most infections are caused by bacteria (especially gram negative), but viru ... | 2008 | 19071255 |
| [the changing patterns and the associated factors of microbial pathogens in ventilator-associated pneumonia in a respiratory intensive care unit from 1995 to 2004]. | to investigate the changing patterns and associated factors of microbial pathogens which caused ventilator-associated pneumonia (vap) in our respiratory intensive care unit (ricu) from 1995 to 2004. | 2008 | 19080406 |
| opportunity knocks. | | 2008 | 19086123 |
| morphological and genetic analysis of three bacteriophages of serratia marcescens isolated from environmental water. | increases in multidrug-resistant strains of serratia marcescens are of great concern in pediatrics, especially in neonatal intensive care units. in the search for bacteriophages to control infectious diseases caused by multidrug-resistant s. marcescens, three phages (ksp20, ksp90, and ksp100) were isolated from environmental water and were characterized morphologically and genetically. ksp20 and ksp90 belonged to morphotype a1 of the family myoviridae, and ksp100 belonged to morphotype c3 of the ... | 2009 | 19087204 |
| effects of electric polarization of indium tin oxide (ito) and polypyrrole on biofilm formation. | the influence of electric polarization on primary adhesion and on biofilm formation was investigated. as substrata, indium tin oxide (ito) and polypyrrole coatings were used because of their electric conductivity. the materials were polarized from -600 mv to +600 mv, switching every 60 seconds. control was non-polarized substrata. primary adhesion under this regime was not strongly influenced, however, the morphology of the primary biofilm was obviously different from that of the control. biofil ... | 2008 | 19092192 |
| selenite resistant rhizobacteria stimulate seo(3) (2-) phytoextraction by brassica juncea in bioaugmented water-filtering artificial beds. | selenium is a trace metalloid of global environmental concern. the boundary among its essentiality, deficiency, and toxicity is narrow and mainly depends on the chemical forms and concentrations in which this element occurs. different plant species-including brassica juncea-have been shown to play a significant role in se removal from soil as well as water bodies. furthermore, the interactions between such plants, showing natural capabilities of metal uptake and their rhizospheric microbial comm ... | 2009 | 19104867 |
| prevalence of tetracycline resistance genes in greek seawater habitats. | the presence of selected tetracycline resistance (tcr) genes was studied in different greek seawater habitats, originated from wastewater treatment facilities, fishfarm, and coastal environments. the methods employed included assessment of the presence of twelve gene clusters by pcr, followed by hybridization with specific probes, in habitat extracted dna, tc(r) bacteria, and exogenous isolated plasmids conferring tcr. the direct dna-based analysis showed that tet(a) and tet(k) genes were detect ... | 2008 | 19107391 |
| probable trimethoprim/sulfamethoxazole-induced higher-level gait disorder and nocturnal delirium in an elderly man. | to report a case of probable trimethoprim/sulfamethoxazole (tmp/smx)-induced higher-level gait disorder (hlgd) and nocturnal delirium in an elderly patient on high-dose oral therapy. | 2009 | 19109207 |
| melanonychia caused by stenotrophomonas maltophilia. | | 2009 | 19120402 |
| genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils. | arsenic is known as a toxic metalloid, which primarily exists in inorganic form [as(iii) and as(v)] and can be transformed by microbial redox processes in the natural environment. as(iii) is much more toxic and mobile than as(v), hence microbial arsenic redox transformation has a major impact on arsenic toxicity and mobility which can greatly influence the human health. our main purpose was to investigate the distribution and diversity of microbial arsenite-resistant species in three different a ... | 2009 | 19128515 |
| tigecycline for treatment of nosocomial-acquired pneumonia possibly caused by multi-drug resistant strains of stenotrophomonas maltophilia. | | 2008 | 19129079 |
| [respiratory tract fluid microbiology in an intensive care unit]. | to know patterns of antimicrobial resistance of bacterial isolates from tracheal aspirates in an intensive care unit and to evaluate the cases of ventilator-associated pneumonia. | 2008 | 19133212 |
| carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a canadian mill. | forty-one bacterial strains isolated from the headbox water of two machines in a canadian paper mill were associated with the genera asticcacaulis, acidovorax, bacillus, exiguobacterium, hydrogenophaga, pseudomonas, pseudoxanthomonas, staphylococcus, stenotrophomonas based on the sequence of their 16s rrna genes. the metabolic profile of these strains were determined using biolog ecoplate, and the bacteria were divided into four metabolic groups. metabolic profiles of the bacterial communities c ... | 2009 | 19137341 |
| characterization of a novel stenotrophomonas isolate with high keratinase activity and purification of the enzyme. | a feather-degrading bacterium was isolated from poultry decomposition feathers in china. the strain, named l1, showed significant feather-degrading activity because it grew and reproduced quickly on basal medium containing 10 g/l of native feather as the source of energy, carbon, and nitrogen. according to the phenotypic characteristics and 16s rrna profile, the isolate belongs to stenotrophomonas maltophilia. keratinase activity of the isolate was determined during cultivation on raw feathers a ... | 2009 | 19137342 |
| pediatric infective endocarditis: has staphylococcus aureus overtaken viridans group streptococci as the predominant etiological agent? | viridans group streptococci (vgs) have traditionally been the most common etiological agents of infective endocarditis (ie). advances in cardiovascular surgery and the increasing use of long-term central venous catheters may have altered the epidemiology of pediatric ie. | 2008 | 19145264 |
| comparison of pulsed-field gel electrophoresis and three rep-pcr methods for evaluating the genetic relatedness of stenotrophomonas maltophilia isolates. | in this study, three facile repetitive-sequence pcr (rep-pcr) techniques have been compared with the pulsed-field gel electrophoresis (pfge) method for differentiating the genetic relatedness of clinical stenotrophomonas maltophilia isolates. | 2008 | 19146527 |
| modified nitrocefin-edta method to differentially quantify the induced l1 and l2 beta-lactamases in stenotrophomonas maltophilia. | to estimate the ethylene diamine tetraacetic acid (edta) concentration at which the l1 enzyme activity in the cell extracts of stenotrophomonas maltophilia can be mostly inhibited. | 2008 | 19146537 |
| cellular and biochemical response to cr(vi) in stenotrophomonas sp. | chromium (cr)-resistant bacteria isolated from a soil with 6 g kg(-1) of cr were identified based on 16s rrna gene sequence analysis as a stenotrophomonas, and designated as jd1. growth of jd1 was accompanied by transformation of cr(vi) to cr(iii) in liquid medium initially containing 300 mg l(-1) cr(vi), the maximum concentration allowing growth. jd1 produced the highest levels of a cr(vi)-binding exopolysaccharide when grown in medium with 100 mg l(-1) cr(vi). the relative exopolysaccharide mo ... | 2009 | 19146572 |
| stenotrophomonas maltophilia induced post-cataract-surgery endophthalmitis: outbreak investigation and clinical courses of 26 patients. | stenotrophomonas maltophilia, a microorganism which colonizes plastic material, is a rare causative agent of iatrogenic endophthalmitis. | 2009 | 19148575 |