| complete genomic sequence for an avian group g rotavirus from south africa. | we report the first complete sequence for an avian group g rotavirus (rvg) genome from africa, which is the third publically available rvg genome. these rvg genomes are highly diverse, especially in their vp4, vp7, nsp4, and nsp3 segments, indicating that rvg diversity is comparable to that of rotavirus a. | 2015 | 25767240 |
| unexpected spreading of g12p[8] rotavirus strains among young children in a small area of central italy. | rotavirus gastroenteritis is associated mainly with the five genotypes g1,3,4,9p[8] and g2p[4] that are common worldwide, but emerging strains including g6, g8, and g12 are also reported sporadically. g12p[8] rotavirus was observed unexpectedly to spread in a limited area of italy during the rotavirus surveillance season 2012-2013. all strains were genotyped for vp7 and vp4 and subjected to phylogenetic analysis. amino acid sequences of antigenic regions were compared with vaccine and field stra ... | 2015 | 25758365 |
| human rotavirus group a serotypes causing gastroenteritis in children less than 5 years and hiv-infected adults in viwandani slum, nairobi. | rotavirus remains a leading cause of acute gastroenteritis in children worldwide with an estimated 2000 deaths each day in developing countries. due to hiv/aids scourge in kenya, it is possible that rotavirus-related gastroenteritis has been aggravated in adults. the global alliance for immunizations has ranked rotavirus infection a priority for vaccine, and, to ensure its success, there is a need to document the local strain(s) circulating in different regions. | 2015 | 25733783 |
| h2 genotypes of g4p[6], g5p[7], and g9[23] porcine rotaviruses show super-short rna electropherotypes. | during group a rotavirus (rva) surveillance of pig farms in japan, we detected three rva strains (g4p[6], g5p[7], and g9p[23] genotypes), which showed super-short rna patterns by polyacrylamide gel electrophoresis, in samples from a healthy eight-day-old pig and two pigs of seven and eight days old with diarrhea from three farms. reverse transcription pcr and sequencing revealed that the full-length nsp5 gene of these strains contained 952 or 945 nucleotides, which is consistent with their super ... | 2015 | 25724331 |
| whole-genome sequence analysis of g3 and g14 equine group a rotaviruses isolated in the late 1990s and 2009-2010. | equine group a rotavirus (rva) g3p[12] and g14p[12] strains cause gastroenteritis in foals worldwide. both of these strains have been co-circulating in japan since g14p[12] strains emerged in the late 1990s. although it is important to comprehensively understand the evolution of rva strains, whole-genome sequence data on recent equine rva strains in japan are lacking. therefore, in this study, whole-genome analysis of 23 equine rva isolates from the late 1990s and 2009-2010 and the vaccine strai ... | 2015 | 25708838 |
| changing patterns of rotavirus strains circulating in ireland: re-emergence of g2p[4] and identification of novel genotypes in ireland. | worldwide, group a rotavirus (rva) is recognized as the most common aetiological agent of acute diarrheal disease in children. one hundred and ninety seven positive faecal samples were obtained from patients between 2006 and 2008. reverse transcriptase polymerase chain reaction (rt-pcr) was used to amplify the vp7 and vp4 gene segments of these samples, and g and p typing was carried out subsequently. the most common strain type was g1p[8], and the emergent global g9-type was identified in both ... | 2015 | 25704492 |
| rotavirus strains in neglected animal species including lambs, goats and camelids. | surveillance of rotavirus infections and circulating strains in small ruminants (i.e. lambs, goats and camelids) has been a neglected research area in the past. however, recent years that have seen an intensification of surveillance in humans and livestock animals, where vaccines to reduce disease burden caused by rotavirus a (rva) are available, led to the efforts to better understand the epidemiology, ecology and evolution of rva strains in other hosts, including lambs, goats and camelids. the ... | 2014 | 25674588 |
| epidemiological and clinical studies of rotavirus-induced diarrhea in china from 1994-2013. | group a rotavirus infections cause diarrhea in infants and primarily occur in winter. these viruses are characterized by the viral structural proteins vp7 (g subtypes) and vp4 (p subtypes). current treatments employ vaccines combined with symptomatic treatments. through a review of published papers from 2003-2013, which included articles, theses, dissertations and academic conference proceedings in pubmed and cnki (china national knowledge infrastructure), we examined the epidemiological and cli ... | 2014 | 25668671 |
| prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. | the objective of this study is to compare the prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. from 110 stool specimens of children with acute diarrhea and using rt-pcr, 35 samples (31.8 %) were positive for human rotavirus group a and 15 samples (13.6 %) were positive for human rotavirus group c. from 96 samples collected from zenin wastewater treatment plant over a 2-year period (november 2009-october 2011) and using rt-pcr, rotavirus group a was detected ... | 2015 | 25665825 |
| molecular epidemiology of group a rotavirus among children admitted to hospital in salto, uruguay, 2011-2012: first detection of the emerging genotype g12. | group a rotavirus (rva) is the most important etiologic agent of infant acute gastroenteritis (age) worldwide. detection and molecular characterization of rva in salto department, northwestern region of uruguay, was conducted on 175 clinical samples, being 153 stool and 22 vomit samples, collected from hospitalized children with age, between 0-15 years old, from two hospitals of salto city during 2011 and 2012. rva was detected and genotyped by seminested multiplex rt-pcr in order to determine g ... | 2015 | 25650154 |
| molecular characterization of equine rotaviruses isolated in europe in 2013: implications for vaccination. | equine group a rotavirus (rvas) mainly cause disease in foals under the age of 3 months. only sporadic data are available on the circulation of rvas in equine populations in europe. in this study, 65 diarrheic samples from foals under 4 months of age were collected in belgium (n=32), germany (n=17), slovenia (n=5), sweden (n=4), hungary (n=3), italy (n=2), france (n=1) and the netherlands (n=1). forty percent of these samples (n=26) were found to be rva positive by a quantitative rt-pcr assay. t ... | 2015 | 25637313 |
| rotavirus and adenovirus gastroenteritis: time series analysis. | this study investigated the effects of changes in weather conditions (monthly average temperature, monthly minimum temperature, monthly average humidity) on rotavirus and adenovirus gastroenteritis frequency and whether there was a seasonal correlation. | 2015 | 25625610 |
| genetic diversity in three bovine-like human g8p[14] and g10p[14] rotaviruses suggests independent interspecies transmission events. | the group a rotavirus (rva) p[14] genotype has been detected sporadically in humans and is thought to be acquired through zoonotic transmission. the present study describes the full-length genome analysis of two g8p[14] and one g10p[14] human rvas detected in italy. the strains possessed the typical bovine-like i2-r2-c2-m2-a3/a11-n2-t6-e2-h3 genotype constellation. all the segments of the two g8p[14] rvas were most closely related to bovine(-like) strains but were relatively distant to each othe ... | 2015 | 25614586 |
| diversity of rotavirus strain circulated in gwangju, republic of korea. | the introduction of new rotavirus vaccines into the public sphere makes it necessary to maintain constant surveillance and to heighten public awareness of the appearance of new rotavirus strains. we describe the molecular epidemiology of circulating rotavirus strains after vaccine introduction. | 2014 | 25562046 |
| detection and genetic characterization of porcine group a rotaviruses in asymptomatic pigs in smallholder farms in east africa: predominance of p[8] genotype resembling human strains. | viral enteritis is a serious problem accounting for deaths in neonatal animals and humans worldwide. the absence of surveillance programs and diagnostic laboratory facilities have resulted in a lack of data on rotavirus associated diarrheas in pigs in east africa. here we describe the incidence of group a rotavirus (rva) infections in asymptomatic young pigs in east africa. of the 446 samples examined, 26.2% (117/446) were positive for rva. more nursing piglets (78.7%) shed rva than weaned (32.9 ... | 2015 | 25541378 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25507599 |
| full genome sequence of a reassortant human g9p[4] rotavirus strain. | this is a report of the complete genomic sequence of a reassortant rotavirus group a g9-p[4]-i2-r2-c2-m2-a2-n2-t2-e6-h2 strain designated rva/human-wt/usa/ lb1562/2010/g9p[4]. | 2014 | 25502675 |
| relationship between viral detection and turbidity in a watershed contaminated with group a rotavirus. | enteric viruses are present in aquatic environments due to contamination by raw sewage, even in the absence of fecal coliforms, which are considered to be significant indicators when it comes to microbial water quality assessment. this study investigated the presence of group a rotavirus (rva) in surface water from a river basin in minas gerais, brazil, assessing the influence of the urbanization, the rainfall, and the microbiological and physico-chemical parameters regarding water quality. fort ... | 2015 | 25471713 |
| full genome characterization of human rotavirus a strains isolated in cameroon, 2010-2011: diverse combinations of the g and p genes and lack of reassortment of the backbone genes. | over the past few years whole genome sequencing of rotaviruses has become a routine laboratory method in many strain surveillance studies. to study the molecular evolutionary pattern of representative cameroonian rotavirus a (rva) strains, the semiconductor sequencing approach was used following random amplification of genomic rna. in total, 31 rva strains collected during 2010-2011 in three cameroonian study sites located 120 to 1240 km from each other were sequenced and analyzed. sequence anal ... | 2014 | 25460824 |
| whole genomic analysis of porcine g10p[5] rotavirus strain p343 provides evidence for bovine-to-porcine interspecies transmission. | porcine group a rotavirus (rva) strain p343 (rva/pig-tc/tha/p343/1991/g10p[5]) was suggested to have vp7 and vp4 genes of bovine origin. in order to obtain precise information on the exact origin and evolution of this unusual porcine strain, the remaining nine genes (vp6, vp1-3, and nsp1-5) of strain p343 were sequenced and analyzed in the present study. on whole genomic analysis, strain p343 was found to have a bovine rva-like genotype constellation (g10-p[5]-i2-r2-c2-m2-a3-n2-t6-e2-h3) differe ... | 2014 | 25457370 |
| an outbreak of acute gastroenteritis associated with group a rotavirus in long-term care facility in slovenia. | in april 2013, an outbreak of acute gastroenteritis was reported in a residential long-term care facility (ltcf) in ljubljana. we carried out an outbreak investigation to describe the outbreak, identify factors associated with contracting infection and to recommend control measures. | 2015 | 25447968 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25427349 |
| molecular epidemiology of rotavirus in cats in the united kingdom. | rotaviruses are leading causes of gastroenteritis in the young of many species. molecular epidemiological studies in children suggest that interspecies transmission contributes to rotavirus strain diversity in people. however, population-based studies of rotaviruses in animals are few. we investigated the prevalence, risk factors for infection, and genetic diversity of rotavirus a in a cross-sectional survey of cats housed within 25 rescue catteries across the united kingdom. morning litter tray ... | 2015 | 25411173 |
| acute diarrhea in adults consulting a general practitioner in france during winter: incidence, clinical characteristics, management and risk factors. | data describing the epidemiology and management of viral acute diarrhea (ad) in adults are scant. the objective of this study was to identify the incidence, clinical characteristics, management and risk factors of winter viral ad in adults. | 2014 | 25358721 |
| isothermal cross-priming amplification implementation study. | cross-priming amplification (cpa) was evaluated for the early detection of norovirus (nv), rotavirus a (rv-a), enteric adenovirus (eadv) and astrovirus (astv). the analytical sensitivity of the cpa assay was 10(3) copies ml(-1) for nv, rv-a and astv detection and 10(4) copies ml(-1) for eadv detection. for each of the four pathogens, the positive detection rate by cpa was similar to real-time pcr methods and higher than the rate observed in an elisa. the detection coincidence rates of cpa and rt ... | 2015 | 25346035 |
| detection of rare g3p[19] group a rotavirus in human patient, italy. | infection with a rare g3p[19] rotavirus a strain was identified in an immunosuppressed patient in italy. the strain showed a p[19] viral protein 4 gene and a complete au-1-like genomic constellation. phylogenetic analyses showed high nucleotide identity between this strain and g3p[19] rotavirus a strains from asia, indicating possible reassortment events. | 2014 | 25340712 |
| analysis of rotavirus genotypes in korea during 2013: an increase in the g2p[4] genotype after the introduction of rotavirus vaccines. | group a rotavirus is the leading cause of acute gastroenteritis in children worldwide. we investigated g and p genotypes of group a rotavirus strains isolated from patients during 2013 and investigated which genotypes were identified from vaccinated patients. | 2014 | 25312273 |
| detection of avian-like rotavirus a vp4 from a calf in japan. | a total of 568 normal feces from calves on a beef farm in fukui prefecture, japan, in 2011-2012 were examined by rt-semi-nested pcr for rotavirus a (rva) vp4 genes. through partial sequencing and blast analyses of 84 vp4-positive specimens, we identified an avian-like rva strain, n2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. phylogenetic analysis also revealed a close genetic relationship between n2342 and avian rvas, suggest ... | 2015 | 25311984 |
| a bovine g8p[1] group a rotavirus isolated from an asymptomatically infected dog. | group a rotaviruses (rvas) are enteric pathogens with well-documented zoonotic transmissions to humans. the segmented genome of the virus enables reassortment events which might alter host susceptibility and/or disease course. genetic analysis of rotavirus in dogs has so far only revealed rvas with the vp7 and vp4 genome constellation g3p[3]. rva g3p[3] have also been found in cats, humans, monkeys and bats. in the present study, we described an unusual rva of genotype g8p[1] which was isolated ... | 2015 | 25304653 |
| [evaluation of real-time multiplex pcr effectiveness for group a rotavirus genotyping]. | evaluate resolution and diagnostic significance of real-time multiplex pcr (mp rt-pcr) as a platform for group a rotavirus g/p genotyping test-systems. | 2014 | 25286527 |
| [evolutionary relationships of g3 garv isolated from pigs and humans in lulong county, hebei province, china]. | this study aimed to amplify major genome segments (vp7, vp4, vp6, vp2 and nsp2-5) of porcine g3 group a rotavirus (garv) llz212 isolated in our laboratory, determine their genotypes, and explore the evolutionary relationships between g3 garv strains isolated from humans and pigs in lulong county, hebei province, china. major genome segments of seven garv strains were amplified by reverse transcription-polymerase chain reaction (rt-pcr) and the segments were sequenced. the genome segments of seve ... | 2014 | 25272597 |
| prevalence and genomic characterization of g2p[4] group a rotavirus strains during monovalent vaccine introduction in brazil. | this study aims to: estimate the prevalence of g2p[4] rotaviruses in brazil between 2001-2011 from patients with acute gastroenteritis; perform phylogenetic analyses of g2p[4] brazilian strains (from vaccinated and non-vaccinated children) based on vp7 and vp8(∗) encoding genes and analyze the antigenic regions of these proteins comparing with rv1; and assess the full genetic background of eleven selected brazilian strains. the g2p[4] detection rate among rva positive samples was 0/157 in 2001, ... | 2014 | 25239525 |
| a possible packaging signal in the rotavirus genome. | group a rotavirus (rva), an etiological agent of gastroenteritis in young mammals and birds, possesses a genome of 11 double-stranded rna segments. although it is believed that the rva virion contains one copy of each genomic segment and that the positive-strand rna (+rna) is incorporated into the core shell, the packaging mechanisms of rva are not well understood. here, packaging signals of rva were searched for by analyzing genomic sequences of mammalian and avian rva, which are considered to ... | 2014 | 25224974 |
| noroviruses associated with outbreaks of acute gastroenteritis in the state of rio grande do sul, brazil, 2004-2011. | acute gastroenteritis norovirus (nov) in a country of continental dimensions like brazil has resulted in under-reporting of the number of outbreaks, as well as the genotypes associated. | 2014 | 25223919 |
| rotavirus genotypes in belarus, 2008-2012. | this study describes group a rotavirus (rva) genotype prevalence in belarus from 2008 to 2012. in 2008, data from 3 sites in belarus (brest, mogilev, minsk) indicated that g4p[8] was the predominant genotype. data from minsk (2008-2012) showed that g4p[8] was the predominant rva genotype in all years except in 2011 when g3p[8] was most frequently detected. other rva genotypes common in europe (g1p[8], g2p[4]) were detected each year of the study. this study reveals the dominance of genotype g4p[ ... | 2014 | 25218086 |
| molecular surveillance of rotavirus infection in bangui, central african republic, october 2011-september 2013. | the world health organization (who) recommends the introduction of rotavirus vaccine in the immunization program of all countries. in the central african republic (car), sentinel surveillance for rotavirus gastroenteritis was established in 2011 by the ministry of health, with the support of the surveillance en afrique centrale project (survac). the purpose of this study was to assess the burden of rotavirus gastroenteritis and to identify rotavirus strains circulating in car before the introduc ... | 2014 | 25193563 |
| metagenomic identification of novel enteric viruses in urban wild rats and genome characterization of a group a rotavirus. | rats are known as reservoirs and vectors for several zoonotic pathogens. however, information on the viruses shed by urban wild rats that could pose a zoonotic risk to human health is scare. here, intestinal contents from 20 wild norway rats (rattus norvegicus) collected in the city of berlin, germany, were subjected to metagenomic analysis of viral nucleic acids. the determined faecal viromes of rats consisted of a variety of known and unknown viruses, and were highly variable among the individ ... | 2014 | 25121550 |
| whole genomic analysis of human g12p[6] and g12p[8] rotavirus strains that have emerged in kenya: identification of porcine-like nsp4 genes. | g12 rotaviruses are globally emerging rotavirus strains causing severe childhood diarrhea. however, the whole genomes of only a few g12 strains have been fully sequenced and analyzed, of which only one g12p[4] and one g12p[6] are from africa. in this study, we sequenced and characterized the complete genomes of three g12 strains (rva/human-tc/ken/kdh633/2010/g12p[6], rva/human-tc/ken/kdh651/2010/g12p[8], and rva/human-tc/ken/kdh684/2010/g12p[6]) identified in three stool specimens from children ... | 2014 | 25111611 |
| molecular epidemiology of rotavirus in children and animals and characterization of an unusual g10p[15] strain associated with bovine diarrhea in south india. | rotaviruses are enteric pathogens causing acute, watery, dehydrating diarrhea in various host species, including birds and mammals. this study collected data on the disease burden and strain prevalence of group a rotavirus in animals and humans in vellore and investigated interspecies transmission by comparison of circulating genotypes. stool samples from children aged less than 5 years, admitted to the hospital between january 2003 and may 2006 for diarrhea and diarrheal samples from animals th ... | 2014 | 25091687 |
| characterisation of rotavirus strains identified in adolescents and adults with acute gastroenteritis highlights circulation of non-typeable strains: 2008-2012. | group-a rotavirus (rv) is the main causative agent of acute gastroenteritis in children < 5 years of age. its role as a pathogen in adults needs to be monitored. the aim of this study was to characterise the group-a rv strains that cause infections of acute gastroenteritis in adolescents and adults and determine the temporal variations in the circulating strains during 2008-2012 in continuation of an earlier study conducted in 2004-2007, in pune, india. | 2014 | 25091684 |
| group a rotavirus gastroenteritis in older children and adults at a hospital in southern india. | there is limited data on the spectrum and prevalence of rotavirus genotypes in older children and adults in asia. this pilot study conducted between november 2012 and april 2013 tested for rotavirus in older children (>12 years of age), and adults with gastroenteritis from southern india. stool samples from patients who were hospitalized or attended out-patient units with diarrhea were screened for rotavirus using premier™ rotaclone(®). confirmatory testing was by another antigen detection sandw ... | 2014 | 25091677 |
| acute gastroenteritis and enteric viruses in hospitalised children in southern brazil: aetiology, seasonality and clinical outcomes. | viral acute gastroenteritis (ag) is a significant cause of hospitalisation in children younger than five years. group a rotavirus (rva) is responsible for 30% of these cases. following the introduction of rva immunisation in brazil in 2006, a decreased circulation of this virus has been observed. however, ag remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. we conducted a prospectiv ... | 2014 | 25075782 |
| human group a rotavirus infections in children in denmark: detection of reassortant g9 strains and zoonotic p[14] strains. | one of the leading causes of severe childhood gastroenteritis are group a rotaviruses, and they have been found to be associated with ∼40% of the annual gastroenteritis-associated hospitalizations in young danish children <5years of age (fischer et al., 2011). in this study, we investigated the diversity of rotavirus strains circulating among young children <5years of age, presenting with gastroenteritis disease either at the general practitioner or in the hospital, during the period 2009-2013. ... | 2014 | 25038295 |
| gastroenteric virus dissemination and influence of rainfall events in urban beaches in brazil. | this study was conducted to assess rotavirus a (rv-a), genogroup ii (gii) norovirus (nov), and human adenovirus (hadv) dissemination in recreational water in an urban beach located in the city of rio de janeiro and their persistence during rainfall events. | 2014 | 24980661 |
| rotavirus surveillance in kisangani, the democratic republic of the congo, reveals a high number of unusual genotypes and gene segments of animal origin in non-vaccinated symptomatic children. | group a rotavirus (rva) infections form a major public health problem, especially in low-income countries like the democratic republic of the congo (cod). however, limited data on rva diversity is available from sub-saharan africa in general and the cod in particular. therefore, the first aim of this study was to determine the genetic diversity of 99 rvas detected during 2007-2010 in kisangani, cod. the predominant g-type was g1 (39%) and the most predominant p-type was p[6] (53%). a total of ei ... | 2014 | 24968018 |
| complete genome analysis of a rare group a rotavirus, g11p[25], isolated from a child in mumbai, india, reveals interspecies transmission and reassortment with human rotavirus strains. | hospital-based rotavirus surveillance was carried out in mumbai during 2005-2009. an isolate (b08299) with a rare genotype combination (g11p[25]) was detected. the present study was undertaken to characterize the complete genome of the isolate. b08299 exhibited a g11-p[25]-i12-r1-c1-m1-a1-n1-t1-e1-h1 genotype constellation. phylogenetic analysis of the 11 gene segments of b08299 revealed that the vp2 and nsp5 genes of b08299 had a human origin, while the vp6 gene represented an i12 genotype of o ... | 2014 | 24951672 |
| [whole genome analysis of human group a rotavirus g9p[8] strains in hebei lulong region, 2009-2011]. | abstract:this study aims to investigate the genetic characteristics of group a rotavirus (garv) g9p[8] strains from infantile diarrhea samples in hebei lulong region from 2009 to 2011. we randomly selected five garv g9p[8] strains in hebei lulong region from 2009 to 2011, amplified the 11 gene fragments of garvs by rt-pcr, and analyz their full-genome sequences by homology and phylogenetic analysis with dnastar and mega. the nucleotide homology between strains ll11131077 and ll11131083 in 2011 w ... | 2014 | 24923163 |
| surveillance of group a rotavirus in buenos aires 2008-2011, long lasting circulation of g2p[4] strains possibly linked to massive monovalent vaccination in the region. | group a rotaviruses (rva) are the most frequent single etiological agents of severe diarrhea in infants. since 2006 rva vaccines have been introduced in national schedules of middle and high income countries with substantial declines in rotavirus associated disease burden. however, surveillance must be maintained to, eventually, detect emerging types or variants selected by the new pressure imposed by vaccination. | 2014 | 24875137 |
| comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves. | although reassortment is one of the most important characteristics of group a rotavirus (rva) evolution, the host range restriction and/or virulence of reassortant rvas remain largely unknown. the porcine 174-1 strain isolated from a diarrheic piglet was identified as a reassortant strain, harboring the same genotype constellation as the previously characterized bovine strain kj56-1. owing to its same genotype constellation, the pathogenicity of the porcine strain 174-1 in piglets and calves was ... | 2014 | 24861840 |
| gastroenteritis outbreaks caused by a ds-1-like g1p[8] rotavirus strain, japan, 2012-2013. | rotavirus a (rva) genotype g1p[8], a hallmark of the wa-like strain, typically contains only genotype 1 genes. however, an unusual rva g1p[8] with genotype 2 genes was recently detected in japan. we determined the complete genomic constellation of this rva. our findings suggest that mixed rvas may be more competitive than once thought. | 2014 | 24856174 |
| molecular detection of human noroviruses in influent and effluent samples from two biological sewage treatment plants in the region of monastir, tunisia. | noroviruses (novs) are responsible for numerous cases of waterborne and foodborne gastroenteritis every year. they are released in the sewage and their detection in this environment can reflect the epidemiology of the viral strains circulating in the community. a three-year (2007-2010) survey was conducted in order to evaluate the presence of human novs using rt-pcr in 518 sewage samples collected at the entrance and exit of two biological sewage treatment plants located in monastir region, tuni ... | 2014 | 24818568 |
| occurrence and characterization of rotavirus a in broilers, layers, and broiler breeders from brazilian poultry farms. | rotaviruses are a major cause of diarrhea in humans and animals, including several mammalian and avian species. using different pcr protocols, we report the occurrence of rotavirus a in 21 (53.84%; 21/39) from 39 fecal pool samples of broilers, layers, and broiler breeders from brazilian avian farms. we typed the g5, g8, g11, g19, and p[31] genotypes. | 2014 | 24758129 |
| distinct evolutionary origins of g12p[8] and g12p[9] group a rotavirus strains circulating in brazil. | g12 group a rotavirus (rva) are currently recognized as a globally emerging genotype and have been described in combination with several p-types. in brazil, g12 rva strains have been described in the southern (2003) and northern (2008-2010) regions, in combination with the p[9] and p[6] genotype, respectively. to date, few complete genomes of g12 rva strains have been described (none from brazilian strains), considering g12p[9] genotype just one strain, rva/human-tc/tha/t152/1998/g12p[9], has th ... | 2014 | 24747965 |
| [prognosis of rotavirus infection in children]. | the aim of the research was to develop the method for predicting the course of rotavirus gastroenteritis in children.under the supervision were 3607 children aged from 9 days to 5 years with the diagnosis of "acute gastroenteritis" and "acute gastroenterocolitis". the diagnosis of rotavirus infection was on the basis of a set of clinical, epidemiological data and the results of para-clinical and bacteriological studies and data of detection of rotavirus antigen strain. genotyping of rotavirus gr ... | 2014 | 24743127 |
| the first caprine rotavirus detected in argentina displays genomic features resembling virus strains infecting members of the bovidae and camelidae. | rotavirus group a (rva) is a major cause of diarrhea in humans and young animals including small ruminants. the purpose of this study was to identify rva in dairy goat kids, and to characterize the complete genomic constellation and genetic relatedness with other rva strains. four out of twenty fecal samples from diarrheic and non-diarrheic goat kids were positive for rva by elisa. a representative sample was selected for further genome analyses. the rva strain rva/goat-wt/arg/0040/2011/g8p[1] d ... | 2014 | 24742949 |
| identification of novel bovine group a rotavirus g15p[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer. | there are few reports describing diarrhea of adult cattle caused by group a rotaviruses. here, we report the identification of a novel bovine group a rotavirus from diarrhea of adult cows. a group a rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in japan in 2013. the comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination g15p[1 ... | 2014 | 24725447 |
| evaluation of the rapid test for human rotavirus a in thai children with acute gastroenteritis. | rapid tests are widely used to detect rotavirus a; however, pediatricians are concerned whether the rapid test can still accurately detect the virus. therefore, this study evaluated the performance of the rotavirus rapid test by comparing it to the one-step rt-pcr method. | 2014 | 24697131 |
| virucidal effect of commercially available disinfectants on equine group a rotavirus. | although many disinfectants are commercially available in the veterinary field, information on the virucidal effects of disinfectants against equine group a rotavirus (rva) is limited. we evaluated the performance of commercially available disinfectants against equine rva. chlorine- and iodine-based disinfectants showed virucidal effects, but these were reduced by the presence of organic matter. glutaraldehyde had a virucidal effect regardless of the presence of organic matter, but the effect wa ... | 2014 | 24681569 |
| sequence and structural analyses of nsp4 proteins from human group a rotavirus strains detected in tunisia. | the nsp4 protein of group a rotavirus (rva) has been recognized as a viral enterotoxin and plays important roles in viral pathogenesis and morphogenesis. domains involved in structural and functional interactions have been proposed mainly based on the simian sa11 strain. | 2014 | 24679587 |
| full-genome sequence of a rare human g3p[9] rotavirus strain. | this is a report of the complete genomic sequence of a rare rotavirus group a g3-p[9]-i2-r2-c2-m2-a3-n2-t1-e2-h3 strain designated rva/human-wt/usa/12us1134/2012/g3p[9]. | 2014 | 24675848 |
| predominance of rotavirus g8 genotype in hospitalized children with acute gastroenteritis in yasuj, iran. | rotavirus infection is the most common cause of gastroenteritis among children worldwide. in this study, we were evaluated the occurrence of rotavirus infection and genotypes causing gastroenteritis among children under 5 years in yasuj, iran. | 2014 | 24668711 |
| diarrhoea-related hospitalizations in children before and after implementation of monovalent rotavirus vaccination in mexico. | to assess, by socioeconomic setting, the effect of nationwide vaccination against species a rotavirus (rva) on childhood diarrhoea-related hospitalizations in mexico. | 2014 | 24623905 |
| surveillance of pathogens causing gastroenteritis and characterization of norovirus and sapovirus strains in shenzhen, china, during 2011. | viral gastroenteritis is one of the most common diseases in humans, and it is primarily caused by rotaviruses (rvs), astroviruses (astvs), adenoviruses (advs), noroviruses (novs), and sapoviruses (savs). in this study, we determined the distribution of viral gastroenteritis and human calicivirus (hucvs) in acute gastroenteritis patients in shenzhen, china, during 2011. real-time rt-pcr was used to detect norovirus (nov), group a rotavirus (rv), adenovirus (adv), and astrovirus (astv). from a tot ... | 2014 | 24610551 |
| complete genomic sequence analyses of the first group a giraffe rotavirus reveals close evolutionary relationship with rotaviruses infecting other members of the artiodactyla. | group a rotaviruses (rva) have been established as significant contributory agents of acute gastroenteritis in young children and many animal species. in 2008, we described the first rva strain detected in a giraffe calf (rva/giraffe-wt/irl/girrv/2008/g10p[11]), presenting with acute diarrhoea. molecular characterisation of the vp7 and vp4 genes revealed the bovine-like genotypes g10 and p[11], respectively. to further investigate the origin of this giraffe rva strain, the 9 remaining gene segme ... | 2014 | 24582453 |
| [research progress of real-time quantitative pcr method for group a rotavirus detection]. | group a rotavirus is one of the most significant etiological agents which causes acute gastroenteritis among infants and young children worldwide. so far, several method which includes electron microscopy (em), enzyme immunoassay (eia), reverse transcription-polymerase chain reaction (rt-pcr)and real-time quantitative pcr has been established for the detection of rotavirus. compared with other methods, real-time quantitative pcr have advantages in specificity, sensitivity, genotyping and quantit ... | 2013 | 24520772 |
| [isolation and characterization of rotavirus from bat]. | bats are considered as important animal reservoirs for many pathogenic viruses to humans. the viral metagenomic analysis was performed to study gut and lung tissues of 30 insectivorous bats collected in yunnan province and 26 reads were noted to group a rotavirus (rva). further rt-pcr screening on bat samples and in vitro viral isolation on cell cultures confirmed the presence of a novel rva, named as rva/bat-tc/myas33/2013/g3p[10], in one of 30 stoliczka's trident bats. the vp7 gene of this str ... | 2013 | 24520769 |
| first molecular detection of group a rotavirus in urban and hospital sewage systems by nested-rt pcr in shiraz, iran. | group a rotaviruses are the most significant cause of acute gastroenteritis in children worldwide. rotaviruses are shed in high numbers and dispersed widely throughout bodies of water in the environment. this represents a significant health hazard for humans, mainly due to the stability of the viruses during wastewater treatment processes. this study was conducted to evaluate the prevalence of rotaviruses, to determine g genotypes of circulating rotaviruses and to assess the efficiency of rotavi ... | 2013 | 24499551 |
| genetic diversity of rotavirus strains circulating in environmental water and bivalve shellfish in thailand. | rotavirus is a common cause of acute diarrhea in young children worldwide. this study investigated the prevalence and molecular characterization of rotavirus in environmental water and oyster samples in thailand. a total of 114 water samples and 110 oyster samples were collected and tested for group a rotavirus using rt-nested pcr. rotavirus genotype was identified by phylogenetic analysis of the vp7 genetic sequences. group a rotavirus was detected in 21 water samples (18.4%) and six oyster sam ... | 2014 | 24469269 |
| circulation of group a rotaviruses among neonates of human, cow and pig: study from assam, a north eastern state of india. | rotavirus (rv) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. therefore, the present study was designed to explore rv incidence in a single approach from human and animal neonates sharing similar environment. altogether, 200 diarrheal samples from children (50), ... | 2013 | 24426283 |
| group a rotaviruses in children with gastroenteritis in a canadian pediatric hospital: the prevaccine era. | a publicly funded, group a rotavirus (rva) vaccination program was implemented in quebec in november 2011. | 2013 | 24421793 |
| diversity of rotavirus strains causing diarrhea in <5 years old chinese children: a systematic review. | we conducted a systematic review of the diversity and fluctuation of group a rotavirus strains circulating in china. | 2014 | 24416267 |
| detection and genetic characterization of human enteric viruses in oyster-associated gastroenteritis outbreaks between 2001 and 2012 in osaka city, japan. | enteric viruses are an important cause of viral food-borne disease. shellfish, especially oysters, are well recognized as a source of food-borne diseases, and oyster-associated gastroenteritis outbreaks have on occasion become international occurrences. in this study, 286 fecal specimens from 88 oyster-associated gastroenteritis outbreaks were examined for the presence of 10 human enteric viruses using antigenic or genetic detection methods in order to determine the prevalence of these infection ... | 2014 | 24415518 |
| molecular analysis of group a rotaviruses detected in adults and adolescents with severe acute gastroenteritis in italy in 2012. | hospital-based surveillance of acute gastroenteritis caused by rotavirus has produced ample knowledge on the infection in children, whereas little is known on rotavirus infection among adults. the italian surveillance program rotanet-italia collected 1,595 samples from patients admitted to hospital with gastroenteritis in 2012. all patients presented with vomiting, diarrhea, fever, and/or abdominal pain. forty-two samples obtained by the rotanet-italia (2.6%) were from adolescents or adults (10- ... | 2014 | 24415411 |
| genetic diversity of group a rotavirus in swine in canada. | group a rotaviruses (rva) in pigs have been poorly investigated in canada. in a continued effort to fill this gap, ten finisher swine farms in quebec, canada, were sampled over a nine-month period. the presence of rva was detected in healthy pigs on all farms investigated during the entire sampling period. the genotypes detected included g2, g5, g9 and g11; p[6], p[7], p[13], p[27] and p[34]; and i5 and i14. the predominant types were g2, p[13] and i5, which is different from previous global rep ... | 2014 | 24402632 |
| detection of picobirnavirus and rotavirus in diarrhoeic faecal samples of cattle and buffalo calves in mumbai metropolis, western india. | in this study 113 diarrhoeic faecal samples obtained from buffalo (n = 68) and cattle (n = 45) calves under 1 years of age were analysed in order to determine the presence of rotavirus infection and the frequency of picobirnavirus excretion. eleven (9.73%) samples positive for group a rotavirus were identified through rna-polyacrylamide gel electrophoresis (rna-page), while 4 (3.53%) samples showed a bisegmented genome with a typical picobirnavirus pattern. this is the first report of picobirnav ... | 2013 | 24362776 |
| prevalence of rotavirus genotypes in south korea in 1989-2009: implications for a nationwide rotavirus vaccine program. | the epidemiology of human group a rotavirus was analyzed by examining genotypic data acquired from 1989 to 2009 in south korea. this information was derived from all the available published articles on rotavirus studies in south korea, retrieved from both the pubmed and koreamed databases. four common g types (g1, g2, g3, and g4) and three common p types (p[8], p[4], and p[6]) accounted for approximately 93% and 99% of the rotavirus reports, respectively. the g9 type was frequently detected afte ... | 2013 | 24348658 |
| epidemiology of rotavirus diarrhea and diversity of rotavirus strains among children less than 5 years of age with acute gastroenteritis in mauritius: june 2008 to december 2010. | rotavirus is an important etiologic agent of diarrhea worldwide and ongoing surveillance is essential to monitor strain diversity. to describe the epidemiology of rotavirus disease and circulating rotavirus strains in mauritius, surveillance for rotavirus diarrhea was conducted at 2 regional hospitals from june 2008 through december 2010. | 2014 | 24343614 |
| hospital-based surveillance for rotavirus gastroenteritis in children younger than 5 years of age in ethiopia: 2007-2012. | rotavirus surveillance was initiated in ethiopia to estimate the burden of rotavirus gastroenteritis in children <5 years of age, to generate data to assist the policy-making process for new vaccine introduction and to monitor impact of vaccination on disease burden after introduction. | 2014 | 24343610 |
| real-time rt-pcr assays to differentiate wild-type group a rotavirus strains from rotarix(®) and rotateq(®) vaccine strains in stool samples. | group a rotaviruses (rva) are the leading cause of severe diarrhea in young children worldwide. two live-attenuated rva vaccines, rotarix(®) and rotateq(®) are recommended by world health organization (who) for routine immunization of all infants. rotarix(®) and rotateq(®) vaccines have substantially reduced rva associated mortality but occasionally have been associated with acute gastroenteritis (age) cases identified in vaccinees and their contacts. high-throughput assays are needed to monitor ... | 2014 | 24342877 |
| complete genotype constellation of human rotavirus group a circulating in thailand, 2008-2011. | this study has identified diverse and re-assorted group a rotavirus (rva) strains by sequence and phylogenetic analysis of the 11 genomic segments. the 22 cases investigated in this study were collected from children with diarrhea between 2008 and 2011. the rva genomic constellations identified in this study were identified as g1-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1 22.7% (5/22); g2-p[4]-i2-r2-c2-m2-a2-n2-t2-e2-h2 27.3% (6/22); g3-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1 18.2% (4/22); g3-p[9]-i3-r3-c3-m3-a3-n ... | 2014 | 24296010 |
| a fut2 gene common polymorphism determines resistance to rotavirus a of the p[8] genotype. | attachment to carbohydrates of the histo-blood group type of several human rotavirus strains (rva) has recently been described. synthesis of these ligands requires a functional fut2 enzyme, suggesting that fut2 null homozygote (ie, nonsecretor) individuals may not be recognized by most human rva strains. whereas such individuals represent 20% of the control population, this retrospective study determined that none of 51 patients infected by p[8] rotavirus strains were nonsecretors. the lack of α ... | 2014 | 24277741 |
| g8p[6] rotaviruses isolated from amerindian children in mato grosso do sul, brazil, during 2009: close relationship of the g and p genes with those of bovine and bat strains. | during the 2009 national group a rotavirus (rva) surveillance, five unusual strains of the human g8p[6] genotype were detected in brazilian indian children with acute gastroenteritis. the aim of this study was to carry out sequence analysis of the two outer capsid proteins (vp4 and vp7) and the inner capsid protein (vp6) of the g8p[6] strains detected in order to provide further information on the genetic relationship between human and animal rva. a total of 68 stool samples, collected in mato g ... | 2014 | 24259191 |
| complete genome analysis of a rare human g3p[9] rotavirus posing as an au-1 like strain. | we performed phylogenetic and sequence analysis by basic local alignment search tool (blast) of a complete human rotavirus (hrv) genome isolated from a hospitalized child with acute gastroenteritis in thailand. | 2013 | 24255863 |
| molecular characterization of human g8p[4] rotavirus strains in italy: proposal of a more complete subclassification of the g8 genotype in three major lineages. | in 2011, two children with acute rotavirus gastroenteritis were hospitalized in sardinia, italy. two rva strains with g8p[4] genotype were detected in their stools, and were named ss56/2011 and ss65/2011. the aim of the study was to characterize these two rare strains, collected within a national rva gastroenteritis surveillance program. eight of the 11 rva genes were sequenced and phylogenetic analysis performed. vp7 amino acid sequence was also analyzed. sequencing of genes encoding the vp4, v ... | 2014 | 24252348 |
| detection of rotavirus in children with acute gastroenteritis in porto velho, rondonia, brazil. | rotavirus group a (rva) infection was ascertained in 591 fecal samples from children 0 to 6 years old with acute gastroenteritis. the vaccination status was also verified in all 591 subjects, with 302 (51.0 % ± 4.0 %) participants fully vaccinated against rotavirus. forty-two of the vaccinated children (13.9 % ± 3.9 %) tested positive for rva infection. of the 289 unvaccinated children (49.0 % ± 4.0 %), 61 (21.1 % ± 4.7 %) had stools positive for rva. this study suggests that the proportion of a ... | 2014 | 24158345 |
| epidemiological and genetic analysis of human group c rotaviruses isolated from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012. | group c rotavirus (gcrv) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. little is known about the yearly changes in the gcrv strains from diarrheal outbreaks. stool samples collected from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012 that were negative for norovirus, sapovirus, and group a rotavirus, were screened for gcrv using a reverse passive hemagglutination method. the gcrv strains were ch ... | 2014 | 24154950 |
| diversity of rotavirus strains circulating in northern brazil after introduction of a rotavirus vaccine: high prevalence of g3p[6] genotype. | rotavirus a (rva) is the most common cause of severe acute gastroenteritis in infants and young children worldwide, causing 453,000 deaths annually. in brazil, the most frequent genotype identified was g1 during almost three decades in the pre-vaccination period; however, after anti-rotavirus vaccine introduction, there was a predominance of g2 genotype. the aim of this study was to determine the g and p genotypes of rotaviruses isolated from children under 5 years of age with acute gastroenteri ... | 2014 | 24136444 |
| detection and molecular characterization of diarrhea causing viruses in single and mixed infections in children: a comparative study between bangladesh and turkey. | the incidence and mortality caused by diarrhea differ among countries. the prevalence of different enteric viruses, their molecular characteristics, and infections with multiple viruses might affect the disease incidence and mortality caused by diarrhea. the objective of this study was to determine the distribution and molecular characteristics of enteric viruses in children with diarrhea in turkey and bangladesh. a total of 288 stool samples that were negative for group a rotavirus were collect ... | 2014 | 24105741 |
| rotavirus in adults, brazil, 2004-2011: g2p[4] dominance and potential impact on vaccination. | the aim of this study was to monitor rotavirus (rv) infections in adults >18 years with acute gastroenteritis during 2004-2011 national brazilian rv surveillance. in addition, to characterize the rv group a (rva) strains in order to gain insight into the supposed vaccine selective pressure imposed to brazilian children population. | 2014 | 24076114 |
| characterization of a novel g3p[3] rotavirus isolated from a lesser horseshoe bat: a distant relative of feline/canine rotaviruses. | bats are considered important animal reservoirs for many viruses pathogenic to humans. an approach based on viral metagenomics was used to study gut specimens from 78 insectivorous bats in yunnan province, china. seventy-four reads were found to be related to group a rotavirus (rva). further reverse transcription-pcr screening and viral isolation on cell cultures confirmed the presence of a novel rva strain, named rva/bat-tc/mslh14/2012/g3p[3], in 1 (6%) of 16 lesser horseshoe bats. full genomic ... | 2013 | 24027312 |
| characterization of the nsp4 gene of group a human rotavirus g1p[8] strains circulating in sapporo, japan from 1987 to 2000. | the genetic diversity of the nsp4 gene of rotavirus g1p[8] strains obtained in sapporo was analyzed, japan from 1987 to 2000. sixty-four strains, which were distributed across the whole study period, were included. all g1p[8] nsp4 genes detected in this study belonged to genotype e1, which divided into at least three lineages. the sapporo rotavirus g1p[8] isolates were found in each lineage. the mean estimated substitution rate was 1.40 × 10(-3) nucleotide substitutions per site per year, which ... | 2014 | 24026858 |
| identification of circulating porcine-human reassortant g4p[6] rotavirus from children with acute diarrhea in china by whole genome analyses. | p[6] group a rotavirus (rva) strains identified in four stool specimens collected from children with acute diarrhea in guangxi province, southern china in 2010, with unknown g type were further analyzed by full genomic analysis. it was revealed by whole genome sequencing that 11 genomic cognate gene segments of these p[6] rva strains shared almost 100% nucleotide identities and all exhibited an identical g4-p[6]-i1-r1-c1-m1-a8-n1-t1-e1-h1 genotype constellation. phylogenetic analyses of vp7, vp1 ... | 2013 | 24012957 |
| detection of rotavirus from hospitalized diarrheic children in uttar pradesh, india. | in the present study 220 stool samples collected from diarrheic children admitted to different hospitals and nursing homes of uttar pradesh and uttarakhand were screened for rotavirus. of 220 diarrheic samples screened 46 samples were found to be positive for rotavirus by rna page. all the isolates exhibited 4-2-3-2 migration pattern suggesting group a rotavirus. both long and short electropherotypes were prevalent in these regions. six different electropherotypes were detected in this study per ... | 2012 | 23997341 |
| global distribution of group a rotavirus strains in horses: a systematic review. | group a rotavirus (rva) is a major cause of diarrhea and diarrhea-related mortality in foals in parts of the world. in addition to careful horse farm management, vaccination is the only known alternative to reduce the rva associated disease burden on horse farms. the precise evaluation of vaccine effectiveness against circulating strains needs enhanced surveillance of equine rvas in areas where vaccine is already available or vaccine introduction is anticipated. therefore, we undertook the overv ... | 2013 | 23994380 |
| molecular characterization of the porcine group a rotavirus nsp2 and nsp5/6 genes from são paulo state, brazil, in 2011/12. | rotaviruses are responsible for the acute diarrhea in various mammalian and avian species. the nonstructural proteins nsp2 and nsp5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral rna replication occur. there are few studies on the genetic diversity of those proteins; thus this study aims at characterizing the diversity of rotavirus based on nsp2 and nsp5 genes in rotaviruses circulati ... | 2013 | 23970830 |
| evidence of natural transmission of group a rotavirus between domestic pigs and wild boars (sus scrofa) in japan. | group a rotaviruses (rvas) are a major cause of acute dehydrating diarrhea in infants and young animals worldwide. rvas have also been detected in several wild and zoo animals, indicating wide susceptibility of wild animals. however, the role of wild animals in the infection cycle of rvas is unclear. wild boars are indigenous in many countries in the world. japanese wild boars (sus scrofa leucomystax) have been migrating close to human habitats in japan, indicating the possibility of natural tra ... | 2013 | 23958658 |
| group a rotavirus and norovirus display sharply distinct seasonal profiles in belém, northern brazil. | several viruses have been associated with acute gastroenteritis (age), and group a rotavirus (rva) and norovirus (nov) are the most prevalent. this study aimed to assess their prevalence among children hospitalised for diarrhoea during a three-year surveillance study. from may 2008-april 2011, overall positivity rates of 21.6% (628/2904) and 35.4% (171/483) were observed for rva and nov, respectively. the seasonality observed indicated distinct patterns when both viruses were compared. this find ... | 2013 | 23903985 |
| novel g10p[14] rotavirus strain, northern territory, australia. | we identified a genotype g10p[14] rotavirus strain in 5 children and 1 adult with acute gastroenteritis from the northern territory, australia. full genome sequence analysis identified an artiodactyl-like (bovine, ovine, and camelid) g10-p[14]-i2-r2-c2-m2-a11-n2-t6-e2-h3 genome constellation. this finding suggests artiodactyl-to-human transmission and strengthens the need to continue rotavirus strain surveillance. | 2013 | 23876354 |
| detection of unusual g6 rotavirus strains in italian children with diarrhoea during the 2011 surveillance season. | two rare g6 rotavirus a (rva) strains, designated as rva/human-wt/ita/cec06/2011/g6p[6] and rva/human-wt/ita/pg05/2011/g6p[9], were identified in stool specimens from children hospitalized in central italy. after pcr genotyping, the samples cec06 and pg05 gave g-ud-p[6] and g-ud-p[9] genotypes, respectively. to determine the g-type and to characterize further the two strains, sequencing of 8 of the 11 genomic segments was performed. cec06 and pg05 strains were found to possess unusual genotype c ... | 2013 | 23868788 |
| rabbit colony infected with a bovine-like g6p[11] rotavirus strain. | group a rotaviruses (rvas) are the main etiological agent of infantile diarrhea in both humans and animals worldwide. a limited number of studies have investigated the molecular characteristics of rva strains in stool specimens of rabbits, with only a few lapine rva strains isolated and (partially) characterized to date. the most common g/p-genotype combinations found in rabbits are g3p[14] and g3p[22]. in this study a rva strain was isolated from the small intestine of a 9-week-old rabbit from ... | 2013 | 23830050 |