| microbial biodegradation of aromatic alkanoic naphthenic acids is affected by the degree of alkyl side chain branching. | naphthenic acids (nas) occur naturally in oil sands and enter the environment through natural and anthropogenic processes. nas comprise toxic carboxylic acids that are difficult to degrade. information on na biodegradation mechanisms is limited, and there are no studies on alkyl branched aromatic alkanoic acid biodegradation, despite their contribution to na toxicity and recalcitrance. increased alkyl side chain branching has been proposed to explain na recalcitrance. using soil enrichments, we ... | 2010 | 20962873 |
| production of metabolites in the biodegradation of phenanthrene, fluoranthene and pyrene by the mixed culture of mycobacterium sp. and sphingomonas sp. | the effects of the mixed culture of mycobacterium sp. strain a1-pyr and sphingomonas sp. strain pheb4 on the degradation characteristics of single polycyclic aromatic hydrocarbon were investigated. in the mixed bacterial culture, phenanthrene, fluoranthene and pyrene were degraded by 100% at day 3, 71.2% and 50% at day 7, respectively. compared to their respective pure cultures, the degradation of phenanthrene and fluoranthene decreased, but that of pyrene increased significantly. based on gc-ms ... | 2010 | 21036605 |
| triclosan susceptibility and co-metabolism--a comparison for three aerobic pollutant-degrading bacteria. | the antimicrobial agent triclosan is an emerging and persistent environmental pollutant. this study evaluated the susceptibility and biodegradation potential of triclosan by three bacterial strains (sphingomonas wittichii rw1, burkholderia xenovorans lb400 and sphingomonas sp. ph-07) that are able to degrade aromatic pollutants (dibenzofuran, biphenyl and diphenyl ether, respectively) with structural similarities to triclosan. these strains showed less susceptibility to triclosan when grown in c ... | 2010 | 21041079 |
| the impact of environmental contamination with antibiotics on levels of resistance in soil bacteria. | this study examined the effects of tetracycline and streptomycin on microorganisms in three different soil habitats: forest soil, agricultural soil, and compost. these antibiotics are commonly used in both medical and veterinary therapy as well as in the production of plant biomass and until quite recently, the production of animal biomass. microcosms were used as model systems in which the number of microorganisms in environments containing different amounts of antibiotics was analyzed. the min ... | 2010 | 21043273 |
| crystallization and preliminary x-ray diffraction studies of a terminal oxygenase of carbazole 1,9a-dioxygenase from novosphingobium sp. ka1. | carbazole 1,9a-dioxygenase (cardo) is the initial dioxygenase in the carbazole-degradation pathway of novosphingobium sp. ka1. the cardo from ka1 consists of a terminal oxygenase (oxy), a putidaredoxin-type ferredoxin and a ferredoxin reductase. the oxy from novosphingobium sp. ka1 was crystallized at 277 k using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. diffraction data were collected to a resolution of 2.1 å. the crystals belonged to the monoclinic spac ... | 2010 | 21045300 |
| sphingomonas oryziterrae sp. nov. and sphingomonas jinjuensis sp. nov. isolated from the rhizosphere soil of rice (oryza sativa l.). | gram-negative, yellow-orange pigmented and rod-shaped bacterial strains, yc6722t and yc6723t, were isolated from rhizosphere soil samples of rice fields in jinju, korea. strains yc6722t and yc6723t grew optimally at 25-30 °c and at ph 7.0-8.5. the phylogenetic analyses of 16s rrna gene sequences showed that strains yc6722t and yc6723t were most closely related to sphingomonas jaspsi tdma-16t and sphingomonas aquatilis jss7t with 96.6 % and 96.9 % similarities in 16s rrna gene sequence, respectiv ... | 2010 | 21057052 |
| physicochemical parameters influencing coaggregation between the freshwater bacteria sphingomonas natatoria 2.1 and micrococcus luteus 2.13. | the aim of this study was to explore the physicochemical parameters that influence coaggregation between the freshwater bacteria sphingomonas natatoria 2.1 and micrococcus luteus 2.13. using visual coaggregation assays, the effect of different buffers, solutions of differing ionic strength, ph, temperature, and viscosity on the degree of coaggregation was assessed. coaggregation occurred maximally in distilled water but was inhibited when coaggregates were suspended in a commonly-used oral bacte ... | 2010 | 21058055 |
| analysis of the composition and characteristics of culturable endophytic bacteria within subnival plants of the tianshan mountains, northwestern china. | this study first described the composition and characteristics of culturable endophytic bacteria isolated from wild alpine-subnival plant species growing under extreme environmental conditions (i.e., on the border of a glacier with frequently fluctuating and freezing temperatures, strong wind, and high ultraviolet radiation). using a cultivation-dependent approach and 16s rrna gene amplification techniques, 93 bacterial isolates showing different phenotypic properties were obtained from 20 diffe ... | 2011 | 21061126 |
| thny is a ferredoxin reductase-like iron-sulfur flavoprotein that has evolved to function as a regulator of tetralin biodegradation gene expression. | previous genetic studies in sphingomonas macrogolitabida strain tfa have established that expression of genes involved in tetralin biodegradation (thn genes) requires the function of the lysr type activator thnr and also thny. sequence comparison indicated that thny is homologous to bacterial oxygenase-coupled nad(p)h-dependent ferredoxin reductases. however, thny showed substitutions in highly conserved positions of the pyridine nucleotide binding domain of these ferredoxin reductases. thny exp ... | 2010 | 21068394 |
| molecular assessment of microbiota structure and dynamics along mixed olive oil and winery wastewaters biotreatment. | the major parcel of the degradation occurring along wastewater biotreatments is performed either by the native microbiota or by added microbial inocula. the main aim of this study was to apply two fingerprinting methods, temperature gradient gel electrophoresis (tgge) and length heterogeneity-pcr (lh-pcr) analysis of 16s rrna gene fragments, in order to assess the microbiota structure and dynamics during mixed olive oil and winery wastewaters aerobic biotreatment performed in a jet-loop reactor ... | 2010 | 21069557 |
| constitutive expression of vitreoscilla haemoglobin in sphingomonas elodea to improve gellan gum production. | to improve a commercially used strain for gellan production by exogenous vitreoscilla haemoglobin (vhb). | 2010 | 21091594 |
| use of reporter-gene based bacteria to quantify phenanthrene biodegradation and toxicity in soil. | a phenanthrene-degrading bacterium, sphingomonas paucimobilis epa505 was used to construct two fluorescence-based reporter strains. strain d harboring gfp gene was constructed to generate green fluorescence when the strain started to biodegrade phenanthrene. strain s possessing gef gene was designed to die once phenanthrene biodegradation was initiated and thus to lose green fluorescence when visualized by a live/dead cell staining. confocal laser scanning microscopic observation followed by ima ... | 2010 | 21093134 |
| microbial quality and nutritional aspects of norwegian brand waters. | the microbiological quality of the five leading brands of norwegian bottled still waters was investigated. all brands were free for the enteric indicator organisms and named pathogens whose absence is demanded in current quality directives. the relatively nutrient-poor agar r₂a revealed large heterogeneous bacterial populations which grew slowly, or not at all, on clinical media specified for use in substrate-utilization approaches to identification. the main approach used for identification was ... | 2010 | 21095035 |
| isolation and characterization of an isoproturon mineralizing sphingomonas sp. strain sh from a french agricultural soil. | the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (ipu), was found to be rapidly mineralized in an agricultural soil in france that had been periodically exposed to ipu. enrichment cultures from samples of this soil isolated a bacterial strain able to mineralize ipu. 16s rrna sequence analysis showed that this strain belonged to the phylogeny of the genus sphingomonas (96% similarity with sphingomonas sp. jem-14, ab219361) and was designated sphingomonas sp. strain sh. ... | 2010 | 21110068 |
| culture-independent study of the diversity of microbial populations in brines during fermentation of naturally-fermented aloreña green table olives. | aloreña table olives are naturally fermented traditional green olives with a denomination of protection (dop). the present study focused on aloreña table olives manufactured by small and medium enterprises (smes) from valle del guadalhorce (southern spain) under three different conditions (cold storage, and ambient temperature fermentations in small vats and in large fermentation tanks). the microbial load of brines during fermentation was studied by plate counting, and the microbial diversity w ... | 2010 | 21122933 |
| application of the mixture design to optimise the formulation of active consortia to decolorize azo-dye methyl red. | with the aid of analysis software (minitab 14.0), the formulation of pure culture in mineral salts medium (msm) can be optimized for several responses and the best formulation can be obtained. the influence of the different mixtures of three strains in the pure culture in msm on the flavor components in decolorization of methyl red (with initial total cell density fixed at od600 = 1 and in addition of 750 ppm of dye) was studied using equilateral triangle diagram and mixture experimental design ... | 2010 | 21123913 |
| dynamics, diversity and function of endophytic siderophore-producing bacteria in rice. | siderophore production confers to bacteria competitive advantages to colonize plant tissues and to exclude other microorganisms from the same ecological niche. this work shows that the community of endophytic siderophore-producing bacteria (spb) associated to oryza sativa cultivated in uruguayan soils is dynamic and diverse. these bacteria were present in grains, roots, and leaves, and their density fluctuated between log(10) 3.44 and log(10) 5.52 cfu g(-1) fresh weight (fw) during the plant gro ... | 2010 | 21128071 |
| biofilm-forming bacteria with varying tolerance to peracetic acid from a paper machine. | biofilms cause runnability problems in paper machines and are therefore controlled with biocides. peracetic acid is usually effective in preventing bulky biofilms. this study investigated the microbiological status of a paper machine where low concentrations (≤15 ppm active ingredient) of peracetic acid had been used for several years. the paper machine contained a low amount of biofilms. biofilm-forming bacteria from this environment were isolated and characterized by 16s rrna gene sequencing, ... | 2010 | 21161323 |
| usefulness of platelet (plt) pathogen inactivation technology: a case of sphingomonas paucimobilis inactivation in an apheresis plt unit. | | 2010 | 21182632 |
| biodegradation of leuco derivatives of triphenylmethane dyes by sphingomonas sp. cm9. | a leuco derivatives of triphenylmethane dyes degrading bacterium, strain cm9, was isolated from an aquafarm field. based on morphology, physiologic tests, 16s rdna sequence, and phylogenetic characteristics, it was identified as sphingomonas sp. this strain was capable of degrading leucomalachite green (lmg), leucocrystal violet and leucobasic fuchsin completely. the relationship between bacterium growth and lmg degradation suggested that strain cm9 could use lmg as the sole source of carbon. th ... | 2010 | 21188476 |
| isolation and identification of soil bacteria growing at the expense of arbuscular mycorrhizal fungi. | soil-microorganism symbioses are of fundamental importance for plant adaptation to the environment. research in microbial ecology has revealed that some soil bacteria are associated with arbuscular mycorrhizal fungi (amf). however, these interactions may be much more complex than originally thought. to assess the type of bacteria associated with amf, we initially isolated spores of glomus irregulare from an agrostis stolonifera rhizosphere. the spores were washed with sterile water and plated on ... | 2011 | 21219415 |
| sphingomonas ginsenosidimutans sp. nov., with ginsenoside converting activity. | the gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated gsoil 1429(t) was isolated from the soil of ginseng cultivating field of pocheon province in south korea. this bacterium was characterized in order to determine its taxonomic position by using the polyphasic approach. strain gsoil 1429(t) grew well at 25-37°c and at ph 7.0 on r2a and nutrient agar without nacl supplement. strain gsoil 1429(t) had -glucosidase activity, which wa ... | 2010 | 21221931 |
| sphingomonas hunanensis sp. nov., isolated from forest soil. | a novel gram-negative, catalase- and oxidase-positive, strictly aerobic, non spore-forming, rod-shaped bacterium, designated strain jsm 083058(t), was isolated from non-saline forest soil in hunan province, china. growth occurred with 0-8% (w/v) nacl (optimum, 0.5-3%) at ph 6.0-10.0 (optimum, ph 7.0) and at 5-35°c (optimum, 25-30°c). phylogenetic analysis based on 16s rrna gene sequences indicated that strain jsm 083058(t) fell within the cluster comprising species of the genus sphingomonas, clu ... | 2011 | 21225342 |
| cloning and knockout of phytoene desaturase gene in sphingomonas elodea atcc 31461 for economic recovery of gellan gum. | a gene encoding phytoene desaturase (crti) in the carotenoid biosynthetic pathway of sphingomonas elodea atcc 31461, an industrial gellan gum-producing strain, was cloned and identified. this gene is predicted to encode a 492-amino acid protein with significant homology to the phytoene desaturase of other carotenogenic organisms. knockout of crti gene blocked yellow carotenoid pigment synthesis and resulted in the accumulation of colorless phytoene, confirming that it encodes phytoene desaturase ... | 2011 | 21229377 |
| crystal structure of bacterial cell-surface alginate-binding protein with an m75 peptidase motif. | a gram-negative sphingomonas sp. a1 directly incorporates alginate polysaccharide into the cytoplasm via the cell-surface pit and abc transporter. a cell-surface alginate-binding protein, algp7, functions as a concentrator of the polysaccharide in the pit. based on the primary structure and genetic organization in the bacterial genome, algp7 was found to be homologous to an m75 peptidase motif-containing efeo, a component of a ferrous ion transporter. despite the presence of an m75 peptidase mot ... | 2011 | 21238429 |
| biodegradation of bromoamine acid using combined airlift loop reactor and biological activated carbon. | the biodegradation of bromoamine acid (baa) in a combined airlift loop reactor (alr) and biological activated carbon (bac) system was investigated. the results showed that the alr using sphingomonas xenophaga as inoculum and granular activated carbon (gac) as carrier, could run steadily for over 3 months at less than 950 mg l(-1) baa. and the efficiencies of baa decolorization and cod removal in alr reached about 90% and 50% within 12h, respectively. when it was further aerated for another 12h, ... | 2010 | 21247761 |
| activity and viability of polycyclic aromatic hydrocarbon-degrading sphingomonas sp. lb126 in a dc-electrical field typical for electrobioremediation measures. | there has been growing interest in employing electro-bioremediation, a hybrid technology of bioremediation and electrokinetics for the treatment of contaminated soil. knowledge however on the effect of weak electrokinetic conditions on the activity and viability of pollutant-degrading microorganisms is scarce. here we present data about the influence of direct current (dc) on the membrane integrity, adenosine triphosphate (atp) pools, physico-chemical cell surface properties, degradation kinetic ... | 2008 | 21261821 |
| rhizoremediation of lindane by root-colonizing sphingomonas. | we used a two-step enrichment approach to isolate root-colonizing hexachlorocyclohexane (hch)-degrading microorganisms. the first step consists of the use of classical liquid enrichment to isolate γ-hch degraders. the γ-hch-degrading microbes were attached in mass to corn seeds sown in soil with γ-hch, and after plant development we rescued bacteria growing on root tips. bacteria were then subjected to a second enrichment round in which growth on liquid medium with γ-hch and inoculation of corn ... | 2008 | 21261825 |
| molecular and biochemical characterization of the tetralin degradation pathway in rhodococcus sp. strain tfb. | the tetralin biodegradation pathway in rhodococcus sp. strain tfb, a gram-positive bacterium resistant to genetic manipulation, was characterized using a proteomic approach. relative protein expression in cell free extracts from tetralin- and glucose-grown cells was compared using the 2d-dige technique. identification of proteins specifically expressed in tetralin-grown cells was used to characterize a complete set of genes involved in tetralin degradation by reverse genetics. we propose a tetra ... | 2009 | 21261920 |
| heterosubunit composition and crystal structures of a novel bacterial m16b metallopeptidase. | three subfamilies of metallopeptidase family m16 enzymes--m16a, m16b, and m16c--are widely distributed among eukaryotes and prokaryotes. sph2681, a periplasmic m16b protein found in sphingomonas sp. strain a1, contains an hxxeh motif essential for zn(2+) binding and catalytic activity. sph2682 is another member of m16b, which lacks the metal-binding motif but conserves an active-site r/y pair commonly found in the c-terminal half of m16 enzymes. two genes coding for sph2681 and sph2682 assemble ... | 2011 | 21262231 |
| [diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province]. | to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china. | 2010 | 21268889 |
| [sphingomonas paucimobilis infection with underlying submandibular sialolithiasis]. | sphingomonas paucimobilis (s. paucimobilis), is a gram-negative, aerobic, non-fermentative, oxidase (+) and catalase (+) bacterium. although s. paucimobilis is isolated very rarely, it can cause both nosocomial and community-acquired infections. a patient admitted to our clinic had a complaint of swelling in the right mandibular region and pain increasing while eating for the previous week. bimanual palpation revealed a painful swelling of 1x1x1 cm in size inside the right wharton's duct. via ma ... | 2011 | 21303318 |
| diversity of endophytic bacteria within nodules of the sphaerophysa salsula in different regions of loess plateau in china. | a total of 115 endophytic bacteria were isolated from root nodules of the wild legume sphaerophysa salsula grown in two ecological regions of loess plateau in china. the genetic diversity and phylogeny of the strains were revealed by restriction fragment length polymorphism and sequencing of 16s rrna gene and enterobacterial repetitive intergenic consensus-pcr. their symbiotic capacity was checked by nodulation tests and analysis of nifh gene sequence. this is the first systematic study on endop ... | 2011 | 21303396 |
| bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications. | distinct from most alginate-assimilating bacteria that secrete polysaccharide lyases extracellularly, a gram-negative bacterium, sphingomonas sp. a1 (strain a1), can directly incorporate alginate into its cytoplasm, without degradation, through a "superchannel" consisting of a mouth-like pit on the cell surface, periplasmic binding proteins, and a cytoplasmic membrane-bound atp-binding cassette transporter. flagellin homologues function as cell surface alginate receptors essential for expressing ... | 2010 | 21326935 |
| a rare case of peripartum endogenous bacterial endophthalmitis. | we describe the case of a healthy, pregnant female who developed endogenous endophthalmitis at the time of delivery, and discuss the possible mechanism of infection and the management of this case. a 26-year-old asian woman presented with a 3-week history of visual deterioration and pain in the right eye. there was no history of ocular trauma or surgery. the ocular symptoms developed one day after vaginal delivery of a healthy baby. the pregnancy had been uncomplicated until premature rupture of ... | 2011 | 21327678 |
| culture-dependent and culture-independent assessment of bacteria in the apple phyllosphere. | bacterial communities in the apple phyllosphere were examined quantitatively and qualitatively by applying culture-dependent and culture-independent methods. | 2011 | 21332895 |
| effects of diverse water pipe materials on bacterial communities and water quality in the annular reactor. | to investigate the effects of pipe materials on biofilm accumulation and water quality, an annular reactor with the sample coupons of four pipe materials (steel, copper, stainless steel, and polyvinyl chloride) was operated under hydraulic conditions similar to a real plumbing system for 15 months. the bacterial concentrations were substantially increased in the steel and copper reactors with progression of corrosion, whereas those in stainless steel (sts) and polyvinyl chloride (pvc) reactors w ... | 2011 | 21364292 |
| pyrosequence analysis of bacterial communities in aerobic bioreactors treating polycyclic aromatic hydrocarbon-contaminated soil. | two aerobic, lab-scale, slurry-phase bioreactors were used to examine the biodegradation of polycyclic aromatic hydrocarbons (pahs) in contaminated soil and the associated bacterial communities. the two bioreactors were operated under semi-continuous (draw-and-fill) conditions at a residence time of 35 days, but one was fed weekly and the other monthly. most of the quantified pahs, including high-molecular-weight compounds, were removed to a greater extent in the weekly-fed bioreactor, which ach ... | 2011 | 21369833 |
| continuous ambulatory peritoneal dialysis (capd) associated peritonitis in a child: a rare case of peritonitis caused by sphingomonous paucimobilis. | first identified in 1977, sphingomonous paucimobilis has emerged as an opportunistic human pathogen. it is primarily known to cause a range of mostly nosocomial, non-life-threatening infections that typically are easily treated by antibiotic therapy. sources of its isolation linked to clinical disease include blood, spinal fluid and leg ulcers. it has also been reported as a rare cause of peritonitis in patients on continuous ambulatory peritoneal dialysis. we present a case of a child with peri ... | 2011 | 21375172 |
| biodiversity and geochemistry of an extremely acidic, low-temperature subterranean environment sustained by chemolithotrophy. | the geochemical dynamics and composition of microbial communities within a low-temperature (ôê+8.5-¦c), long-abandoned (>ôçâ90 years) underground pyrite mine (cae coch, located in north wales) were investigated. surface water percolating through fractures in the residual pyrite ore body that forms the roof of the mine becomes extremely acidic and iron-enriched due to microbially accelerated oxidative dissolution of the sulfide mineral. water droplets on the mine roof were found to host a very li ... | 2011 | 21382147 |
| the lin genes for +¦-hexachlorocyclohexane degradation in sphingomonas sp. mm-1 proved to be dispersed across multiple plasmids. | a +¦-hexachlorocyclohexane (hch)-degrading bacterium, sphingomonas sp. mm-1, was isolated from soil contaminated with hch isomers. cultivation of mm-1 in the presence of +¦-hch led to the detection of five +¦-hch metabolites, +¦-pentachlorocyclohexene, 2,5-dichloro-2,5-cyclohexadiene-1,4-diol, 2,5-dichlorohydroquinone, 1,2,4-trichlorobenzene, and 2,5-dichlorophenol, strongly suggesting that mm-1 has the lin genes for +¦-hch degradation originally identified in the well-studied +¦-hch-degrading s ... | 2011 | 21389627 |
| (r,s)-dichlorprop herbicide in agricultural soil induces proliferation and expression of multiple dioxygenase-encoding genes in the indigenous microbial community. | we investigated the effect of (r,s)-dichlorprop herbicide addition to soil microcosms on the degrading indigenous microbial community by targeting multiple a-ketoglutarate-dependent (a-kg) dioxygenase-encoding genes (rdpa, sdpa and tfda group i) at both gene and transcript level. the soil microbial community responded with high growth of potential degraders as measured by the abundance of dioxygenase-encoding genes using quantitative real-time pcr (qpcr). rdpa dna was not detectable in unamended ... | 2011 | 21418495 |
| interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms. | it is well established that legionella pneumophila is a waterborne pathogen; by contrast, the mode of helicobacter pylori transmission remains unknown but water seems to play an important role. this work aims to study the influence of five microorganisms isolated from drinking water biofilms on the survival and integration of both of these pathogens into biofilms. | 2011 | 21418578 |
| protection of arabidopsis thaliana against leaf-pathogenic pseudomonas syringae by sphingomonas strains in a controlled model system. | diverse bacterial taxa live in association with plants without causing deleterious effects. previous analyses of phyllosphere communities revealed the predominance of few bacterial genera on healthy dicotyl plants, provoking the question of whether these commensals play a particular role in plant protection. here, we tested two of them, methylobacterium and sphingomonas, with respect to their ability to diminish disease symptom formation and the proliferation of the foliar plant pathogen pseudom ... | 2011 | 21421777 |
| effect of fe-pd bimetallic nanoparticles on sphingomonas sp. ph-07 and a nano-bio hybrid process for triclosan degradation. | in this study, we have evaluated the effect of palladium-iron bimetallic nanoparticles (nfe-pd) on diphenyl ether (de) degrading bacterial strain sphingomonas sp. ph-07 as well as a sequential nano-bio hybrid process with nfe-pd as catalytic reductant and ph-07 as biocatalyst for degradation of triclosan. strain ph-07 grew well in the presence of nfe-pd up to 0.1g/l in minimal salts medium with de as carbon source. in aqueous system, tcs (17.3 µm) was completely dechlorinated within 2h by nfe-pd ... | 2011 | 21429741 |
| computational study on the interaction of a ring-hydroxylating dioxygenase from sphingomonas chy-1 with pahs. | the massive computational resources available in the framework of a grid paradigm approach represent an emerging tool in the bioinformatics field. in this paper, we used the above approach in the rapid determination of the interactions between the ring-hydroxylating dioxygenase, comprised six enzymatic subunits, and polycyclic aromatic hydrocarbons (pahs) in their optimal positions. the results were obtained by simulating enzyme dynamics at 300 k through molecular dynamics calculations. for the ... | 2011 | 21441049 |
| characterization of an exo-ß-1,3-d: -galactanase from sphingomonas sp. 24t and its application to structural analysis of larch wood arabinogalactan. | a type ii arabinogalactan-degrading enzyme, termed exo-1,3-gal, was purified to homogeneity from the culture filtrate of sphingomonas sp. 24t. it has an apparent molecular mass of 48 kda by sds-page. exo-1,3-gal was stable from ph 3 to 10 and at temperatures up to 40 °c. the optimum ph and temperature for enzyme activity were ph 6 to 7 and 50 °c, respectively. galactose was released from ß-1,3-d: -galactan and ß-1,3-d: -galactooligosaccharides by the action of exo-1,3-gal, indicating that the en ... | 2011 | 21452032 |
| modeling sorption and biodegradation of phenanthrene in mangrove sediment slurry. | a mathematical model, combining both sorption and biodegradation process, was developed to predict the biodegradation of phenanthrene by sphingomonas sp. in different sediment slurries. the model includes two sorption parameters, α (the partition coefficient) and 1/k (the diffusion resistance); a kinetic parameter k (the first order rate constant); and a sediment parameter, a(v) (the specific sediment surface area in unit volume of slurry). these parameters were evaluated and verified in three t ... | 2011 | 21474240 |
| interactions of leptospira with environmental bacteria from surface water. | leptospires can persist for months in nutrient-poor aqueous environments prior to transmission to a mammalian host. interactions with environmental bacteria and biofilm formation are possible mechanisms of persistence of leptospires in the environment. bacteria isolated from rivers in the ecuadorian rainforest were tested for their ability to support leptospiral viability. we found that co-culture with sphingomonas spp., but not flavobacterium spp. or delftia spp., enabled survival of l. biflexa ... | 2011 | 21479795 |
| temperature and biotic factors influence bacterial communities associated with the cyanobacterium microcystis sp. | cyanobacterial blooms represent a nutritious niche for associated bacteria including potential pathogens for humans as well as livestock. we investigated bacterial community composition associated with microcystis sp. using different approaches: batch experiments on microcystis sp. or its enriched exudates, field enclosures (dialysis bags) and field sampling during natural blooms in freshwaters. bacterial community composition associated with microcystis sp. differed significantly with temperatu ... | 2011 | 21492362 |
| culturable bacterial diversity at the princess elisabeth station (utsteinen, sør rondane mountains, east antarctica) harbours many new taxa. | we studied the culturable heterotrophic bacterial diversity present at the site of the new princess elisabeth station at utsteinen (dronning maud land, east antarctica) before construction. about 800 isolates were picked from two terrestrial microbial mat samples after incubation on several growth media at different temperatures. they were grouped using rep-pcr fingerprinting and partial 16s rrna gene sequencing. phylogenetic analysis of the complete 16s rrna gene sequences of 93 representatives ... | 2011 | 21501941 |
| composition and distribution of bacteria in an operating rainwater harvesting tank. | in this study, we investigated the phylogenetic distribution of the bacteria present in an operating rainwater tank by denaturing gradient gel electrophoresis (dgge), and compared the bacterial composition in rainwater and biofilm from the inlet and outlet of the tank. seventeen species were identified, the dgge profiles of which showed a clear difference between the planktonic bacterial community and the community in the biofilm. most of the bacteria were closely related to fresh water, soil, a ... | 2011 | 21508560 |
| role of a conserved arginine residue during catalysis in serine palmitoyltransferase. | all sphingolipid-producing organisms require the pyridoxal 5'-phosphate (plp)-dependent serine palmitoyltransferase (spt) to catalyse the first reaction on the de novo sphingolipid biosynthetic pathway. spt is a member of the alpha oxoamine synthase (aos) family that catalyses a claisen-like condensation of palmitoyl-coa and l-serine to form 3-ketodihydrosphingosine (kds). protein sequence alignment across various species reveals an arginine residue, not involved in plp binding, to be strictly c ... | 2011 | 21514297 |
| contrasting effects of ultraviolet radiation on the growth efficiency of freshwater bacteria. | in this study, we tested the hypothesis that the growth efficiency of freshwater bacteria is differentially affected by ultraviolet radiation (uvr, 280-400 nm) as mediated through changes in their production and respiration rates. five bacterial strains affiliated to alphaproteobacteria, betaproteobacteria, gammaproteobacteria, and actinobacteria were isolated from different freshwater habitats and exposed in the laboratory to photosynthetically active radiation (par) and par + uvr, or kept in t ... | 2011 | 21516253 |
| application of the knowledge-based approach to strain selection for a bioaugmentation process of phenanthrene- and cr(vi)-contaminated soil. | aims: the objective of this study was to apply the knowledge-based approach to the selection of an inoculum to be used in bioaugmentation processes to facilitate phenanthrene degradation in phenanthrene- and cr(vi)-co-contaminated soils. methods and results: the bacterial community composition of phenanthrene and phenanthrene- and cr(vi)-co-contaminated microcosms, determined by denaturing gradient gel electrophoresis analysis, showed that members of the sphingomonadaceae family were the predo ... | 2011 | 21518157 |
| identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal rna gene sequence analysis. | clin microbiol infect 2011; 17: 776-779 abstract: dna sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16s and internal transcribed spacer (its) ribosomal rna gene sequence analysis. pcr was positive for 24 of 99 samples tested. both culture and 16s rdna sequence analysis identified pseudomonas aeruginosa, streptococci and enterobacteriaceae. isolates misidentified as burkholderia cepacia by biochemical tes ... | 2011 | 21521414 |
| risk factors associated with sphingomonas paucimobilis infection. | background: sphingomonas paucimobilis is rarely isolated from clinical specimens and it is associated with a great variety of infections. the aim of this study is to investigate the microbiological and clinical features of s paucimobilis infection in southern taiwan. methods: s paucimobilis isolates from the microbiology laboratory of chi-mei medical center and their relevant clinical data from october 2005 to october 2009 were retrospectively reviewed. results: a total of 55 patients with docum ... | 2011 | 21524965 |
| a diverse bacterial community in an anoxic quinoline-degrading bioreactor determined by using pyrosequencing and clone library analysis. | there is a concern of whether the structure and diversity of a microbial community can be effectively revealed by short-length pyrosequencing reads. in this study, we performed a microbial community analysis on a sample from a high-efficiency denitrifying quinoline-degrading bioreactor and compared the results generated by pyrosequencing with those generated by clone library technology. by both technologies, 16s rrna gene analysis indicated that the bacteria in the sample were closely related to ... | 2011 | 21538111 |
| sphingomonas jejuensis sp. nov., isolated from marine sponge hymeniacidon flavia. | a gram-negative, non-motile, rod shaped, and orange-pigmented chemoheterotrophic bacterium, strain ms-31(t) was isolated from the marine sponge hymeniacidon flavia, collected from near jeju island, korea. the strain ms-31(t) was subjected to a polyphasic taxonomic study. the phylogenetic analysis based on the 16s rrna gene sequences revealed that the novel isolate could be affiliated within the genus sphingomonas. the strain ms-31(t) showed 95.6% of 16s rrna gene sequence similarity with the mos ... | 2011 | 21538244 |
| transcriptomic analysis of phenanthrene degrading sphingomonas biofilms exposed to environmentally relevant solute and matric stresses. | | 2011 | 21539200 |
| effects of solids retention time on the performance of bioreactors bioaugmented with a 17β-estradiol-utilizing bacterium, sphingomonas strain kc8. | this study investigated the performance of lab-scale sequencing batch reactors (sbrs) that were inoculated with nitrifying activated sludge and bioaugmented with a sphingomonas strain kc8 (a 17β-estradiol-degrading bacterium). the bioaugmented sbrs were supplied with synthetic wastewater (average initial total organic carbon (toc)=175mgl(-1) and average initial ammonia-n=25mgl(-1)) and daily dose of 17β-estradiol (1mgl(-1)) and operated under three solid retention times (srts) of 5, 10, and 20d. ... | 2011 | 21543102 |
| innate and cytokine-driven signals, rather than microbial antigens, dominate in natural killer t cell activation during microbial infection. | invariant natural killer t cells (inkt cells) are critical for host defense against a variety of microbial pathogens. however, the central question of how inkt cells are activated by microbes has not been fully explained. the example of adaptive mhc-restricted t cells, studies using synthetic pharmacological α-galactosylceramides, and the recent discovery of microbial inkt cell ligands have all suggested that recognition of foreign lipid antigens is the main driver for inkt cell activation durin ... | 2011 | 21555485 |
| diversity of bacteria at healthy human conjunctiva. | purpose. ocular surface (os) microbiota contributes to infectious and autoimmune diseases of the eye. comprehensive analysis of microbial diversity at the os has been impossible because of the limitations of conventional cultivation techniques. this pilot study aimed to explore true diversity of human os microbiota using dna sequencing-based detection and identification of bacteria. methods. composition of the bacterial community was characterized using deep sequencing of the 16s rrna gene ampli ... | 2011 | 21571682 |
| bacterial communities constructed in artificial consortia of bacteria and chlorella vulgaris. | we established artificial consortia of bacteria inoculated from soil and a green alga, chlorella vulgaris nies-227. the bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. the community of bacteria formed in the consortia included those phylogenetically closely related to the genera phenylobacterium, brevundimonas, phyllobacterium, afipia, sphingomonas, sandaracinobacter, ramlibacter, ralstonia, ce ... | 2010 | 21576850 |
| abundance and community structure of sphingomonads in leaf residues and nearby bulk soil. | we examined the abundance and community structure of sphingomonads in the decaying leaf residues of eight plant species as well as the nearby soil, by 16s rrna gene-based real-time pcr and denaturing gradient gel electrophoresis. in the leaf residues, the sphingomonads generally accumulated to high levels, comprising approximately 15% of the total bacteria, and formed a community structure related to sampling locations. at least within the time period studied, their abundance in leaf residues ch ... | 2010 | 21576871 |
| low temperature s(0) biomineralization at a supraglacial spring system in the canadian high arctic. | elemental sulfur (s(0) ) is deposited each summer onto surface ice at borup fiord pass on ellesmere island, canada, when high concentrations of aqueous h(2) s are discharged from a supraglacial spring system. 16s rrna gene clone libraries generated from sulfur deposits were dominated by β-proteobacteria, particularly ralstonia sp. sulfur-cycling micro-organisms such as thiomicrospira sp., and ε-proteobacteria such as sulfuricurvales and sulfurovumales spp. were also abundant. concurrent cultivat ... | 2011 | 21592302 |
| genome sequence of sphingomonas sp. s17 isolated from an alkaline, hyperarsenic and hypersaline volcanic associated lake near 4000 meters above sea level in the argentinean puna. | the high-altitude andean lakes (haal) in the argentinian puna-high andes region represent an almost unexplored ecosystem exposed to extreme conditions (high uv irradiation, hypersalinity, drastic temperature changes, desiccation, high ph). here we present the first genome sequence isolated from this extreme environment, a sphingomonas sp. | 2011 | 21602338 |
| patchwork assembly of nag-like nitroarene dioxygenase genes and 3-chlorocatechol degradation cluster for the pathway evolution of 2-chloronitrobenzene catabolism in pseudomonas stutzeri zwlr2-1. | pseudomonas stutzeri zwlr2-1 utilizes 2-chloronitrobenzene (2cnb) as a sole source of carbon, nitrogen and energy. to identify genes involved in this pathway, a 16.2-kb dna fragment containing putative 2-chloronitrobenzene dioxygenase genes was cloned and sequenced. of the products from the 19 open reading frames that resulted from this fragment, cnbac and cnbad exhibited striking identities to the respective α- and β- subunits of the nag-like ring-hydroxylating dioxygenases involved in the meta ... | 2011 | 21602392 |
| immobilization of microbial cells on inner epidermis of onion bulb scale for biosensor application. | inner epidermis of onion bulb scales was used as a natural support for immobilization of microbial cells for biosensor application. a bacterium sphingomonas sp. that hydrolyzes methyl parathion into a chromophoric product, p-nitrophenol (pnp), has been isolated and identified in our laboratory. pnp can be detected by electrochemical and colorimetric methods. whole cells of sphingomonas sp. were immobilized on inner epidermis of onion bulb scale by adsorption followed by cross-linking methods. ce ... | 2011 | 21605968 |
| synergistic effects and physiological responses of selected bacterial isolates from animal feed to four natural antimicrobials and two antibiotics. | abstract in this study, 20 samples from three different sources of animal feed were investigated and six bacterial isolates were identified. the susceptibility of four natural antimicrobials, namely, eugenol, cinnamaldehyde, thymol, and carvacrol, against six of these isolates was determined. carvacrol and eugenol showed better inhibitory effects with larger zones of inhibition. the minimal inhibitory concentration for a range of antibiotics on the susceptibility of two isolates (namely, sphingo ... | 2011 | 21612425 |
| coenzyme q10 production by sphingomonas sp. zute03 with novel precursors isolated from tobacco waste in a two-phase conversion system. | coenzyme q10 (coq10) is a widely used supplement in heart diseases treatment or antioxidative dietary. the microbial production of coq10 was enhanced by addition of solanesol and novel precursors recovered from waste tobacco. the novel precursors were separated by silica gel and identified as alpha-linolenic acid (lna) and butylated hydroxytoluene (bht) based on the effect on coq10 production and gc-ms. the effects of novel precursors on coq10 production by sphingomonas sp. zute03 were further e ... | 2011 | 21617346 |
| physiological traits of the symbiotic bacterium teredinibacter turnerae isolated from the mangrove shipworm neoteredo reynei. | nutrition in the teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria of the teredinibacter clade. the mangrove teredinidae neoteredo reynei is popularly used in the treatment of infectious diseases in the north of brazil. in the present work, the symbionts of n. reynei, which are strictly confined to the host's gills, were conclusively identified as teredinibacter turnerae. symbiont variants obtained in vitro were able to grow using casein a ... | 2009 | 21637522 |
| diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis. | microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ... | 2011 | 21660545 |
| an unexpected gene cluster for downstream degradation of alkylphenols in sphingomonas sp. strain ttnp3. | in silico analysis of nucleotide sequences flanking the recently found hydroquinone dioxygenase in sphingomonas sp. strain ttnp3 revealed a gene cluster that encodes a hydroquinone catabolic pathway. in addition to the two open-reading frames encoding the recently characterized hydroquinone dioxygenase, the cluster consisted of six open-reading frames. we were able to express the three open-reading frames, hqdc, hqdd, and hqde, and demonstrated that the three gene products, hqdc, hqdd, and hqde ... | 2011 | 21755281 |
| dehydration of the off-flavor chemical 2-methylisoborneol by the r-limonene-degrading bacteria pseudomonas sp. strain 19-rlim and sphingomonas sp. strain bir2-rlima. | the terpene 2-methylisoborneol (mib), a major cause of off-flavor in farm-raised catfish and drinking water, is transformed by various different terpene-degrading bacteria. two of these, the r-limonene-degrading strains pseudomonas sp. 19-rlim and sphingomonas sp. bir2-rlima, dehydrated mib with the formation of odorless metabolites 2-methylenebornane and 4-methylcamphene. these metabolites which have a structural resemblance to camphor, could be further transformed by camphor-degrading bacteria ... | 2011 | 21842206 |
| sphingomonas polyaromaticivorans sp. nov., a polycyclic aromatic hydrocarbons degrading-bacterium isolated from water sample of oil port. | a bacterium strain (b2-7t), capable of degrading wide range of polycyclic aromatic hydrocarbon compounds (2-4 rings), was isolated from water sample of botan oil port in xiamen, china. the isolate was gram-negative, short-rod-shaped, aerobic, non-motile, and formed yellow-pigmented colonies on lb medium. cells of strain b2-7t were catalase-positive and oxidase-negative. the optimal growth of strain b2-7t was at ph 7.0, 26 -¦c and 0.5% nacl, respectively. phylogenetic analysis based on 16s rrna g ... | 2011 | 21742816 |
| proposal of sphingomicrobium lutaoense gen. nov. sp. nov., isolated from a coastal hot spring of green island (lutao). | a yellowish pigmented, gram-negative, rod-shaped, non-spore-forming bacterium (strain cc-tbt-3t), was isolated from coastal hot spring of green island (lutao), located off taituang, taiwan, on marine agar 2216. the 16s rrna gene sequence analysis of strain cc-tbt-3t showed a relatively low (< 95.5%) to representatives of the genera novosphingobium, sphingosinicella and sphingomonas of the sphingomonadaceae with novosphingobium soli as the most similar species. beside the relatively low 16s rrna ... | 2011 | 21828023 |
| crystal structure of h2o2-dependent cytochrome p450sp{alpha} with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the {alpha} position. | cytochrome p450(sp+¦) (cyp152b1) isolated from sphingomonas paucimobilis is the first p450 to be classified as a h(2)o(2)-dependent p450. p450(sp+¦) hydroxylates fatty acids with high +¦-regioselectivity. herein we report the crystal structure of p450(sp+¦) with palmitic acid as a substrate at a resolution of 1.65 ôä½. the structure revealed that the c(+¦) of the bound palmitic acid in one of the alternative conformations is 4.5 ôä½ from the heme iron. this conformation explains the highly selec ... | 2011 | 21719702 |
| cloning and characterization of a novel oligoalginate lyase from a newly isolated bacterium sphingomonas sp. mj-3. | a bacterium possessing alginate-degrading activity was isolated from marine brown seaweed soup liquefied by salted and fermented anchovy. the isolated strain was designated as sphingomonas sp. mj-3 based on the analyses of 16s ribosomal dna sequences, 16s-23s internal transcribed spacer region sequences, biochemical characteristics, and cellular fatty acid composition. a novel alginate lyase gene was cloned from genomic dna library and then expressed in escherichia coli. when the deduced amino a ... | 2011 | 21826589 |
| diversity and antibiotic resistance patterns of sphingomonadaceae isolates from drinking water. | sphingomonadaceae (n = 86) were isolated from a drinking water treatment plant (n = 6), tap water (n = 55), cup fillers for dental chairs (n = 21), and a water demineralization filter (n = 4). the bacterial isolates were identified based on analysis of the 16s rrna gene sequence, and intraspecies variation was assessed on the basis of atpd gene sequence analysis. the isolates were identified as members of the genera sphingomonas (n = 27), sphingobium (n = 28), novosphingobium (n = 12), sphingopy ... | 2011 | 21705522 |
| planktonic microbes in the gulf of maine area. | in the gulf of maine area (goma), as elsewhere in the ocean, the organisms of greatest numerical abundance are microbes. viruses in goma are largely cyanophages and bacteriophages, including podoviruses which lack tails. there is also evidence of mimivirus and chlorovirus in the metagenome. bacteria in goma comprise the dominant sar11 phylotype cluster, and other abundant phylotypes such as sar86-like cluster, sar116-like cluster, roseobacter, rhodospirillaceae, acidomicrobidae, flavobacteriales ... | 2011 | 21698243 |
| abundance and diversity of sphingomonas in shenfu petroleum-wastewater irrigation zone, china. | introduction: members of the genus sphingomonas have raised increasing attention due to their ability for polycyclic aromatic hydrocarbon (pah) degradation and their ubiquity in the environment. however, few studies have revealed the ecological information on the abundance and diversity of sphingomonas in the environment. materials and methods: a primer set targeting the sphingomonas 16s rrna gene was designed. the specificity was tested with four petroleum-contaminated soils by construction of ... | 2011 | 21735160 |
| testosterone-mineralizing culture enriched from swine manure: characterization of degradation pathways and microbial community composition. | environmental releases and fate of steroid sex hormones from livestock and wastewater treatment plants are of increasing regulatory concern. despite the detection of these hormones in manures, biosolids, and the environment, little attention has been paid to characterization of fecal bacteria capable of hormone degradation. the enrichments of (swine) manure-borne bacteria capable of aerobic testosterone degradation were prepared and the testosterone mineralization pathway was elucidated. six dna ... | 2011 | 21740029 |
| the effects of metabolite molecules produced by drinking water-isolated bacteria on their single and multispecies biofilms. | the elucidation of the mechanisms by which diverse species survive and interact in drinking water (dw) biofilm communities may allow the identification of new biofilm control strategies. the purpose of the present study was to investigate the effects of metabolite molecules produced by bacteria isolated from dw on biofilm formation. six opportunistic bacteria, viz. acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staph ... | 2011 | 21732713 |
| outbreak of sphingomonas paucimobilis septicemia in a neonatal intensive care unit. | we describe an outbreak of sphingomonas paucimobilis in 13 newborn infants with septicemia and septic shock. fifteen available isolates were obtained from patients and distilled water. pulsed-field gel electrophoresis results pointed out that there was a cross-transmission of s. paucimobilis in eleven patients but these types were not the same the isolate obtained from the distilled water. the outbreak was terminated by taking appropriate control measures for infection and change of source of di ... | 2011 | 21719938 |
| fine tuning by human cd1e of lipid-specific immune responses. | cd1e is a member of the cd1 family that participates in lipid antigen presentation without interacting with the t-cell receptor. it binds lipids in lysosomes and facilitates processing of complex glycolipids, thus promoting editing of lipid antigens. we find that cd1e may positively or negatively affect lipid presentation by cd1b, cd1c, and cd1d. this effect is caused by the capacity of cd1e to facilitate rapid formation of cd1-lipid complexes, as shown for cd1d, and also to accelerate their tur ... | 2011 | 21844346 |
| x-ray structure reveals a new class and provides insight into evolution of alkaline phosphatases. | the alkaline phosphatase (ap) is a bi-metalloenzyme of potential applications in biotechnology and bioremediation, in which phosphate monoesters are nonspecifically hydrolysed under alkaline conditions to yield inorganic phosphate. the hydrolysis occurs through an enzyme intermediate in which the catalytic residue is phosphorylated. the reaction, which also requires a third metal ion, is proposed to proceed through a mechanism of in-line displacement involving a trigonal bipyramidal transition s ... | 2011 | 21829507 |
| poly(aspartate) hydrolases: biochemical properties and applications. | thermally synthesized poly(aspartate) (tpaa) shows potential for use in a wide variety of products and applications as a biodegradable replacement for non-biodegradable polycarboxylates, such as poly(acrylate). the tpaa molecule has unnatural structures, and the relationship between its biodegradability and structures has been investigated. two tpaa-degrading bacteria, sphingomonas sp. kt-1 and pedobacter sp. kp-2, were isolated from river water; from them, two paa-hydrolyzing enzymes, paa hydro ... | 2011 | 21713512 |
| changes in the bacterial community structure and diversity during bamboo retting. | microbial retting is a critical step in obtaining fiber bundles from bamboo culm using indigenous microorganisms. a cultivation-independent technique for monitoring the changes in bacteria community during bamboo retting was applied in this work. this technique involves genetic profiling of pcr-amplified small-subunit rrna and the single-strand conformation polymorphism (sscp) gel analysis of the pcr-amplified 16s rdna fragments. the study revealed that both the structure and the diversity of in ... | 2011 | 21695788 |
| Genome Sequence of the 17{beta}-Estradiol-Utilizing Bacterium Sphingomonas Strain KC8. | Sphingomonas strain KC8 is known for its ability to utilize 17+¦-estradiol, a natural estrogen and an environmental endocrine-disrupting compound, as the sole carbon and energy source. Here, we report the draft genome sequence of the strain KC8 (4,074,265 bp, with a GC content of 63.7%) and major findings from its annotation. | 2011 | 21685282 |
| sphingomonas paucimobilis bacteremia related to intravenous human immunoglobulin injections. | | 2011 | 22075255 |
| sphingomonas endophytica sp. nov., a novel bacterium isolated from artemisia annua l. | a novel bacterium (strain yim 65583(t)) belonging to the genus sphingomonas was isolated from a surface-sterilized tissue of artemisia annua l., which was collected from yunnan province, south-west china. its morphology, physiology, biochemical features were consistent with those members of the genus sphingomonas. 16s rrna gene sequence of strain yim 65583(t) further confirmed that it should be classified as a member of the genus sphingomonas and was most closely related to sphingomonas phyllosp ... | 2011 | 21873515 |
| Sphingomonas alpina sp. nov., a psychrophilic bacterium isolated from alpine soil. | An aerobic, Gram-negative, motile, psychrophilic bacterium, designated strain S8-3T, was isolated from alpine soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S8-3T was related to the genus Sphingomonas and had highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica S213T (98.0 %). 16S RNA gene sequence similarity between strain S8-3T and Sphingomonas paucimobilis ATCC 29837 T (the type species of the genus Sphingomonas) was 93.0 %. Strain S8-3T ... | 2011 | 21856979 |
| molecular phylogeny of the genus dactylopius (hemiptera: dactylopiidae) and identification of the symbiotic bacteria. | phylogenetic analyses, from polymerase chain reaction (pcr)-amplified 12s rrna and 18s rrna gene sequences from cochineal insects of the genus dactylopius present in mexico, showed that d. ceylonicus, d. confusus, and d. opuntiae are closely related. d. coccus constitutes a separate clade, and d. tomentosus is the most distantly related. bacterial 16s rrna sequences from all the dactylopius species sampled showed a common ß-proteobacteria, related to azoarcus, also found in eggs and in bacterio ... | 2010 | 22127169 |
| [Endogenous postpartum panophthalmitis induced by sphingomonas paucimobili]. | Endogenous panophthalmitis is a rare eye disease with purulent necrosis of all the ocular structures. It is a rare but serious condition that occurs when bacteria cross the blood-ocular barrier and multiply within the eye. Although rare, endogenous panophthamitis is a potentially devastating intraocular infection resulting in a poor visual and anatomic prognosis. We present a 39-year-old woman, without any previous history, who developed a endogenous panophthalmitis in the left eye secondary to ... | 2011 | 22003763 |
| growth of chlorella vulgaris and associated bacteria in photobioreactors. | the aim of this study was to test three flat plate photobioreactor configurations for growth of chlorella vulgaris under non-axenic conditions and to characterize and quantify associated bacterial communities. the photobioreactor cultivations were conducted using tap water-based media to introduce background bacterial population. growth of algae was monitored over time with three independent methods. additionally, the quantity and quality of eukaryotes and bacteria were analysed using culture-in ... | 2012 | 21936882 |
| bioconversion of ginsenosides rb(1), rb(2), rc and rd by novel β-glucosidase hydrolyzing outer 3-o glycoside from sphingomonas sp. 2f2: cloning, expression, and enzyme characterization. | a new β-glucosidase gene (bglsp) was cloned from the ginsenoside converting sphingomonas sp. strain 2f2 isolated from the ginseng cultivating filed. the bglsp consisted of 1344 bp (447 amino acid residues) with a predicted molecular mass of 49,399 da. a blast search using the bglsp sequence revealed significant homology to that of glycoside hydrolase superfamily 1. this enzyme was overexpressed in escherichia coli bl21 (de3) using a pet21-mbp (tev) vector system. overexpressed recombinant enzyme ... | 2011 | 21906640 |
| culturable bacterial communities on leaf sheaths and panicles of rice plants in japan. | culturable bacterial communities on rice plants were investigated from 2001 to 2003. in total, 1,394 bacterial isolates were obtained from the uppermost leaf sheaths at 1 month before heading time and from leaf sheaths and panicles at heading time. the average culturable bacterial population on the leaf sheaths was larger at heading time than at 1 month previously. furthermore, the population was significantly larger on panicles than on leaf sheaths, suggesting that the bacterial population is i ... | 2011 | 22081407 |
| Sphingomonas formosensis sp. nov., a polycyclic aromatic hydrocarbons degrading-bacterium isolated from agricultural soil. | In the present study, a yellow pigmented, Gram-negative, short rod-shaped novel bacterium was isolated from agricultural soil located in Yunlin County, Taiwan. Comparative 16S rRNA gene sequence analysis positioned the novel strain in the genus Sphingomonas as an independent lineage adjacent to a subclade containing Sphingomonas fennica K101(T), Sphingomonas histidinilytica UM2(T), Sphingomonas wittichii RW1(T), and Sphingomonas haloaromaticamans A175(T). The 16S rRNA gene sequence analysis of s ... | 2011 | 21873513 |
| optimization of fenton process for decoloration and cod removal in tobacco wastewater and toxicological evaluation of the effluent. | decoloration and chemical oxygen demand (cod) removal in tobacco wastewater by fenton process has been optimized under 25 +/- 2 degrees c. the results showed that the optimal range of conditions were ph 4.13-4.66, fe(2+) 0.29-0.34 g/l and h2o2 > or = 2.73 g/l. within this range, up to 95% of colour and 90% of cod was removed. in an enlarged system, setting the optimal conditions as ph 4.50, fe(2+) 0.34 g/l and h2o2 4.00 g/l, the colour removal rate was 96.03 +/- 2.57%, with cod removal rate of 9 ... | 2011 | 22049737 |