| effect of kallikrein on bull sperm motility in vitro. | the application of kallikrein--a kinin-releasing porcine pancreatic kininogenase--exerted considerable stimulation of bull sperm motility in vitro. the kallikrein was used in two concentrations: 1 and 100 ku/ml semen. both concentrations gave similar effect: at the 4th hour of incubation at room temperature the motility rate of spermatozoa exceeded five and four times, respectively, that of the control samples. higher penetrating ability of bull spermatozoa in cow's estral secretion was also rec ... | 1978 | 30714 |
| release rate of testosterone and estrogens from polydimethylsiloxane implants for extended periods in vivo compared with loss in vitro. | release rates of testosterone, estrone, and estradiol placed in chambers made from polydimethylsiloxane (pds) tubing (dow corning "silastic," 3.35 mm id x 4.65 mm od) were studied in 14 freemartin cattle with minimal or non-detectable endogenous hormone secretion, and in 0.9% saline:methanol (1:1) baths shaken at 38 degree c. eighty-seven implants, varying in length from 2 to 10 cm, were placed in 14 animals for 27 to 235 days. the average release rates +/- standard errors, in microgram/cm/day, ... | 1978 | 30722 |
| phosphorus influence on growth and reproduction of beef cattle. | | 1978 | 30743 |
| effect of epinephrine on acetyl-coa carboxylase in rat epididymal fat tissue. | if acetyl-coa carboxylase in epididymal fat tissue is subject to control by convalent modification as in the case of the liver enzyme, catalytically different forms of carboxylase should exist, independent of polymerization. by treating epididymal fat tissue in culture with epinephrine, we have demonstrated catalytically less active forms of acetyl-coa carboxylase. the catalytically less active forms of the enzyme reacted to antibody with the same efficiency as the active form of carboxylase. ho ... | 1978 | 30775 |
| hemolytic properties of synthetic glycosides. | cholesteryl alpha-l-rhamnopyranoside, tigogenyl alpha-l-rhamnopyranoside, tigogenyl beta-d-fucopyranoside, smilagenyl beta-d-fucopyranoside, cholesteryl beta-maltoside, tigogenyl beta-maltoside, and smilagenyl beta-maltoside were synthesized and characterized. the rhamnosides and fucosides, as well as some other steroid monoglycosides, proved to be extremely insoluble in water. the concentration giving 50% hemolysis, h50, was of the same order of magnitude for all synthetic glycosides. ghost cel ... | 1978 | 30839 |
| modification of noradrenergic hypothalamic system in rat injected with phosphatidylserine liposomes. | | 1978 | 30871 |
| physico-chemical properties of mouse hepatitis virus (mhv-2) grown on dbt cell culture. | some properties of a strain of mouse hepatitis virus, mhv-2, grown on dbt cells were determined using a plaque assay on the cells. viral growth was not inhibited by the presence of actinomycin d or 5-iodo-2-deoxyuridine. mhv-2 was completely inactivated by ether, chloroform, sodium deoxycholate or beta-propiolactone, but showed a moderate resistance to trypsin. heating at 56 c for 30 min did not completely abolish the virus infectivity. the virus was stable after heating at 50 c for 15 min in 1m ... | 1978 | 30881 |
| on the capacity of the beta-oxidation of palmitate and palmitoyl-esters in rat liver mitochondria. | the beta-oxidation of palmitate, palmitoyl-coa and palmitoyl-l-carnitine proceeded at a high rate in isolated rat liver mitochondria. at high concentrations (100 nmol/mg protein) the oxidation of palmitate and palmitoyl-coa was only partly carnitine dependent. all substrates were most rapidly oxidized in the presence of oxaloacetate and state 3 conditions. succinate inhibited beta-oxidation especially in state 4 conditions. beta-oxidation was faster in hypotonic than in isotonic medium both in s ... | 1978 | 31061 |
| inhibition by adriamycin of a metmyoglobin reductase from beef heart. | | 1978 | 31164 |
| absorption of magnesium by the young steer. | 1. steers with rumen and simple duodenal cannulas were allowed to graze pasture or were given diets of dried grass or flaked maize with or without hay. for an experiment a solution or suspension of magnesium chloride, polyethylene glycol (molecular weight 4000) and either 144ce (as cerous chloride) or chromic oxide was added to the rumen with a morning feed. conditions in the rumen were sometimes modified by adding sodium chloride or hydrochloric acid. 2. changes in magnesium:marker in samples o ... | 1978 | 31168 |
| evidence from hydrogen-1 and carbon-13 nuclear magnetic resonance studies that the dissociation rate of oxytocin from bovine neurophysin at neutral ph is slow. | | 1978 | 31171 |
| the sulphatase of ox liver. xxi: kinetic studies of the substrate-induced inactivation of sulphatase a. | the theoretical basis is given for methods of determining the apparent velocity constant, k*, for the substrate-induced inactivation of sulphatase a (aryl-sulphate sulphohydrolase, ec 3.1.6.1) and the initial velocity, vo, of the catalytic reaction. the expression is of the same form as the empirical relationships previously used but the significance of the various terms is clearly established. the method has been applied to the characterisation of the inactivation occurring during the hydrolysi ... | 1978 | 31181 |
| the purification of a bovine kidney enzyme which cleaves melanocyte-stimulating hormone-release inhibiting factor. | an enzyme which catalyzes the hydrolysis of l-prolyl-l-leucylglycinamide, the factor which inhibits the release of melanocyte-stimulating hormone, was purified 189-fold from bovine kidney in a 5% yield. the molecular weight of the enzyme on gel filtration was estimated to be 300 000 and its isoelectric point was found to be ph 4.1. the single component seen on sodium dodecyl sulphate-gel electrophoresis was estimated to have a molecular weight of 56 000, indicating that the native enzyme may be ... | 1978 | 31186 |
| the ph-dependence of the non-specific esterase activity of carboxypeptidase a. | the hydrolysis of the following 6 esters by bovine pancreatic carboxypeptidase a (peptidyl-l-amino-acid hydrolase, ec 3.4.12.2) has been investigated over the range ph 5--10 at 25 degrees c, ionic strength 0.2: ch3co2chrco2h (r = c6h5 (ester 3), c6h5ch2 (ester 4), 4-no2c6h4co2chrco2h (r = c6h5 (ester 5), c6h5ch2 (ester 6), ch3(ch2)2 (ester 7)), ch3ch2co2ch(ch2c6h5)-co2h (ester 9). for each ester the ph dependence of kcat/km indicates that substrate binding is controlled by an acid of pkeh = 9.2 ... | 1978 | 31187 |
| development of a radioreceptor assay for beta-adrenoceptor antagonists in plasma [proceedings]. | | 1978 | 31225 |
| [properties of adenohypophyseal proteinase i]. | | 1978 | 31267 |
| neuroleptic receptors in calf caudate: solubilization by digitonin. | | 1978 | 31289 |
| tryptophanyl-trna synthetase: evidence for an anhydrous bond involved in the tryptophanyl enzyme formation. | | 1978 | 31302 |
| enzyme reactions of atp studied by positional isotope exchange. | reversible gamma-po3 transfer in atp reactions can be recognized by exchange of 18o from the beta,gamma-bridge position to the beta-p-nonbridge positions: (see article). such intramolecular exchange is less demanding for the detection of the bond cleavage than the usual atp:adp isotope exchange because it does not require dissociation of bound adp from the intermediate complex. acyl phosphate intermediates are indicated for the glutamine synthetase and carbamyl-p synthetase reactions by their ex ... | 1978 | 31305 |
| [participation of paramedical personnel in services rendered to cattle breeders]. | | 1978 | 31306 |
| studies on the mosquitoes of bankura district, west bengal: adult populations. | | 1978 | 31333 |
| bovine platelet myosin. iii. properties of mg- and ca-atpase. | | 1978 | 31357 |
| conformation of functionally homologous proteins. turtle and bovine ribonucleases. | | 1979 | 31363 |
| purification of soluble guanylate cyclase from rat lung. | the soluble form of guanylate cyclase from rat lung has been purified approximately 23,000-fold to homogeneity by isoelectric precipitation, gtp-sepharose chromatography, and preparative gel electrophoresis. a single protein-staining band is observed after analytical gel electrophoresis on either 4 or 7.5% polyacrylamide gels. the final purified enzyme has a specific activity of about 700 nmol of cyclic gmp formed/min/mg of protein at 37 degrees c in the presence of 4.8 mm mncl2 and 100 micromet ... | 1979 | 31365 |
| [food hosts of mass mosquito species (diptera, culicidae) in tadzhikistan]. | | 1978 | 31557 |
| [8th international symposium on comparative study of leukemia and related diseases]. | | 1978 | 31731 |
| purification and properties of rennin-like enzyme from aspergillus ochraceus. | an active milk-clotting enzyme was purified some 40-fold from culture supernatant of aspergillus ochraceus. the purification steps included ammonium sulfate precipitation, g-100 sephadex gel filtration, and ion exchange chromatography, using deae cellulose column. the enzyme exhibited milk-clotting activity and proteolytic behaviour, an optimum at ph 6.0 and in the range of 7--8.5, respectively. the purified enzyme was actively proteolytic against casein, haemoglobin, and bovine serum albumin at ... | 1978 | 31745 |
| growth of infective forms of trypanosoma (t.) brucei on buffalo lung and chinese hamster lung tissue culture cells. | infective cultures of trypanosoma (t.) brucei (strain 427) have been initiated and maintained on chinese hamster lung tissue culture cells and buffalo lung tissue culture cells. by changing daily one-third of the rpmi-1640 plus 20% fetal bovine serum medium, the cell numbers can be maintained at 2--4 x 10(6) cells/ml. the cultured trypanosomes on these two tissue culture cell types were infective to mice and morphologically similar to bloodstream slender trypomastigotes in having a subterminal k ... | 1978 | 31773 |
| pancreatic response to intestinal perfusion with lactic acid or acidified albumin. | to test the hypothesis that the permeability of weak acids across the intestinal mucosa affects their ability to stimulate pancreatic bicarbonate output, we compared pancreatic bicarbonate secretion in response to intestinal perfusion with an acid presumed to be permeable to cell membranes, lactic acid (90 daltons), and an acid presumed to be impermeable, acidified bovine serum albumin (about 70,000 daltons). these two substances have similar titration curves from ph 2.00 to ph 4.50. in four con ... | 1978 | 31795 |
| synthesis of quinolinate from d-aspartate in the mammalian liver-escherichia coli quinolinate synthetase system. | | 1978 | 31876 |
| studies on the reaction catalyzed by transport (na, k) adenosine triphosphatase-ii. in vitro and in vivo effects of phenoxybenzamine. | | 1978 | 31879 |
| zymogen activation in serine proteinases. proton magnetic resonance ph titration studies of the two histidines of bovine chymotrypsinogen a and chymotrypsin aalpha. | reversible unfolding of bovine chymotrypsinogen a in 2h2o either by heating at low ph or by exposure to 6 m guanidinium chloride results in the exchange of virtually all the nitrogen-bound hydrogens that give rise to low-field 1h nmr peaks, without significant exchange of the histidyl ring cepsilon1 hydrogens. these preexchange procedures have enabled the resolution of two peaks, using 250-mhz correlation 1h nmr spectroscopy, that are attributed to the two histidyl residues of chymotrypsinogen a ... | 1978 | 31898 |
| active site in zymogens. proton magnetic resonance ph titration curves of histidine-57 in porcine and bovine trypsinogens and in their complexes with bovine pancreatic trypsin inhibitor (kunitz). | | 1978 | 31899 |
| chemical modification of mitochondrial transhydrogenase: evidence for two classes of sulfhydryl groups. | chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (ec 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (nadp) site and another peripheral to the active site. reaction of the peripheral sulfhydryl group with n-ethylmaleimide, or both classes with 5,5'-dithiobis(2-nitrobenzoic acid), completely inactivated transhydrogenase. nadp+ or nadph nearly completely protected against 5,5'-dit ... | 1978 | 31900 |
| acetyl-coa acetyltransferase from bovine liver mitochondria. molecular properties of multiple forms. | bovine liver mitochondrial acetyl-coa acetyltransferase (acetyl-coa:acetyl-coa c-acetyltransferase, ec 2.3.1.9) has been obtained in three forms designated transferase i, a and b on the basis of their elution positions from chromatography on phosphocellulose. all forms have been shown to have a molecular weight of about 152 000, each being composed of four similar subunits. amino acid analysis of transferase a and b, the two major forms, revealed a close relationship between both forms with almo ... | 1978 | 31914 |
| the role of zn(ii) in calf intestinal alkaline phosphatase studied by the influence of chelating agents and chemical modification of histidine residues. | alkaline phosphatase from calf intestine (orthophosphoric-monoester phosphohydrolase (alkaline optimum), ec 3.1.3.1) is reversibly inhibited at ph 8.0 by incubation with chelating agents. complete reactivation may be achieved by stoichiometric addition of zn2+. atomic absorption spectrometry was used to demonstrate the linear correlation between zn2+ content and degree of reactivation. the reversibly inhibited enzyme contained 1 zn2+ per subunit whereas 2 zn2+ were found in both the reactivated ... | 1978 | 31915 |
| collagen-induced platelet aggregation and release. i effects of side-chain modifications and role of arginyl residues. | to investigate the mechanisms governing collagen interaction with blood platelets, the effects of side-chain modifications on collagen-induced platelet aggregation and release of serotonin were studied. since many chemical modifications alter the ability of collagen to form fibers that, according to current theory, may complicate interpretation of data, we eliminated this possibility by using collagen stabilized in a native-type fibrillar structure by treatment with either glutaraldehyde or ultr ... | 1978 | 31929 |
| collagen-induced platelet aggregation and release. ii critical size and structural requirements of collagen. | | 1978 | 31930 |
| studies on the succinate dehydrogenating system. i. kinetics of the succinate dehydrogenase interaction with a semiquindiimine radical of n,n,n',n'-tetramethyl-p-phenylenediamine. | 1. the activities of the soluble reconstitutively active succinate dehydrogenase (ec 1.3.99.1) measured with three artificial electron acceptors, e.g. ferricyanide, phenazine methosulfate and free radical of n,n,n',n'-tetramethyl-p-phenylenediamine (wb), have been compared. the values estimated by extrapolation to infinite acceptor concentration using double reciprocal plots 1/v versus 1/[acceptor] are nearly the same for ferricyanide and phenazine methosulfate and about twice as high for the wb ... | 1979 | 31933 |
| natural plant enzyme inhibitors. vi. studies on trypsin inhibitors of colocasia antiquorum tubers. | a trypsin inhibitor was purified from the tubers of colocasia antiquorum. the inhibitor acted on bovine trypsin, human trypsin and weakly on bovine chymotrypsin. the inhibitor, which had a molecular weight of 40 000, contained trace amounts of carbohydrates. the purified inhibitor was stable over a ph range of 2.0--12.0 and was more thermostable than the crude preparations. trinitrobenzene sulphonate treatment resulted in the inactivation of the inhibitor. chymotrypsin, pepsin and pronase digest ... | 1979 | 31937 |
| effect of salts on d-glycerate dehydrogenase kinetic behavior. | bovine liver d-glycerate dehydrogenase (d-glycerate:nad (nadp) oxidoreductase, ec 1.1.1.29) adapts its kinetic behaviour to a sequential mechanism. the presence of nacl causes an appreciable variation in the km and v values. relative to the both substrates in the hydroxypyruvate/d-glycerate dehydrogenase/nadh system, which does not happen in the d-glycerate/d-glycerate dehydrogenase/nad system. the former system is inhibited by high concentrations of nacl and activated by low salt concentrations ... | 1979 | 31939 |
| nuclear phosphoprotein phosphatase from calf liver. | calf liver nuclear phosphoprotein phosphatase (phosphoprotein phosphohydrolase, ec 3.1.3.16) has been purified approx. 850-fold. the enzyme has a mol. wt. of 34 000 as determined by sds-polyacrylamide gel electrophoresis. the purified enzyme has a ph optimum between 7.0 and 7.5 with phosphophosphorylase, phosphohistones f1 and f2b, and phosphoprotamine as substrates. the enzyme activity towards these substrates follows the order, phosphophosphorylase greater than phosphohistone f1 greater than p ... | 1979 | 31941 |
| 3-hydroxy-3-methylglutaryl coenzyme a reductase activity in beef adrenal cortex. | 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmg-coa reductase) activity (mevalonate:nadp+ oxidoreductase )coa-acylating) ec 1.1.1.34) was demonstrated in beef adrenal cortex. most of the hmg-coa reductase activity is in the microsomal fraction while a small percentage of the activity is associated with the mitochondria, mitochondria purified on a linear sucrose gradient are enriched in hmg-coa reductase and cytochrome c oxidase activities. the reductase present in microsomal preparations fr ... | 1978 | 31970 |
| kinetics of milk lipoprotein lipase. studies with tributyrin. | | 1978 | 32030 |
| the quaternary structure of bovine alpha-crystallin. size and shape studies by sedimentation, small-angle x-ray scattering and quasi-elastic light scattering. | | 1978 | 32031 |
| autoxidation of native oxymyoglobin. kinetic analysis of the ph profile. | the rate of autoxidation of native oxymyoglobin to metmyoglobin has been examined over the ph range of 4.8--12.6 in 0.1 m buffer at 25 degrees c, and some 40 values of the observed first-order rate constant, kobs, are plotted against ph of the solution. in order to understand the kobs--ph profile thus obtained, some mechanistic models are proposed for the autoxidation reaction. the fitting of their rate equations as a function of ph has been examined to the experimental kobs-ph plot by a least-s ... | 1978 | 32032 |
| measurement of beta-adrenoceptor antagonists in biological fluids using a radioreceptor assay. | a new assay for plasma levels of beta-adrenoceptor antagonists is described. the assay depends upon the ability of the drug to compete with a labelled beta-adrenoceptor antagonist (-)-[3h]dihydroalprenolol for beta-adrenoceptor binding sites on lung membranes. the assay is simple to perform, very sensitive and does not require prior extraction of plasma. the assay can also detect bioactive metabolites of these agents and is clearly not limited to a single beta-adrenoceptor antagonist. | 1978 | 32055 |
| cholinergic and adrenergic innervation of the penis artery of the bull: transmitter concentrations and synaptic vesicles. | the penile artery of the bull contained significant amounts of acetylcholine, noradrenaline and dopamine, and its axon profiles contained either numerous small granular or agranular vesicles, as well as some large granular vesicles. in the dorsal metatarsal artery, only noradrenaline and dopamine were detectable, and the axon profiles observed contained numerous small granular vesicles. in the penile artery, the axons with small agranular vesicles, probably cholinergic axons, were in close conta ... | 1978 | 32069 |
| mn2+ inside submitochondrial particles as a tool for studying the functional state of the mitochondrial membrane. | | 1978 | 32073 |
| binding of cholesterol by neisseria gonorrhoeae. | the binding of [1,2-3h]cholesterol to neisseria gonorrhoeae cs-7, pseudomonas aeruginosa, and salmonella typhimurium (smooth and rough strains) was investigated. the kinetics of cholesterol binding to n. gonorrhoeae cs-7 demonstrated that binding occurred slowly with maximum binding by 10 h. under optimum conditions, a large percentage (65%) of the added cholesterol was associated with the cells. chemical fractionation revealed that ca. 98% of the labeled cholesterol was associated with the cell ... | 1978 | 32139 |
| extramitochondrial protein synthesis in calf brain synaptosomes. | isolated synaptosomes of calf brain cortex incorporated labelled amino acids into their mitochondrial, membranous and soluble proteins in an approximate ratio of 1:1:0.5 synaptosomal protein synthesis was sensitive to atp, noradrenaline, cycloheximide and puromycin, and together with mitochondrial protein synthesis, also to chloramphenicol, 2.4-dinitrophenol, kcn and hyperosmotic conditions. the absence of na+ and k+ ions slightly inhibited both synaptosomal and mitochondrial protein synthesis. ... | 1977 | 32146 |
| crystalline reduced nicotinamide adenine dinucleotide phosphate-adrenodoxin reductase from pig adrenocortical mitochondria. essential histidyl and cysteinyl residues of the nadph-binding site and environment of the adrenodoxin-binding site. | pig nadph-adrenodoxin reductase was crystallized from pig adrenocortical mitochondria and its physicochemical properties were investigated. pig nadph-adrenodoxin reductase is a typical flavoprotein. its optical absorption spectrum showed peaks at 272, 377, and 450 nm in the oxidized form. the adrenodoxin reductase contained one fad per mol. the molecular weight was 49,000. the isoelectric points of the adrenodoxin reductase and its complex with adrenodoxin were 5.3 and 4.6, respectively. pig nad ... | 1978 | 32168 |
| purification and enzymatic properties of gamma-glutamyltransferase from bovine colostrum. | | 1978 | 32176 |
| bovine gamma-glutamyltransferases. immunological identity and varying affinities for concanavalin a. | | 1978 | 32177 |
| on the heterogeneity of catalase from goat liver. purification and characterization. | | 1978 | 32178 |
| acid and alkaline phosphatases in a brain tubulin preparation. | | 1979 | 32229 |
| conductivity changes during enzyme inhibitor interaction [proceedings]. | | 1978 | 32330 |
| a method to estimate binding constants at variable protein concentrations. | the association constants of the binding of chlorpromazine and imipramine to serum albumin at low saturation of the protein were determined by a new experimental approach with the protein concentration rather than the ligand concentration being varied. this approach is suitable for estimating binding constants in systems with one class of binding sites. in addition, the method is proposed to complement conventional binding studies of systems with two classes of binding constant with higher accur ... | 1979 | 32379 |
| regulation by histamine of cyclic nucleotide levels in sympathetic ganglia. | an investigation has been carried out of the role of histamine h1- and h2-receptors in the control of cyclic guanosine 3':5'-monophosphate (cgmp) and cyclic adenosine 3':5'-monophosphate (camp) levels in blocks of bovine superior cervical ganglion. the data suggest that activation of h1-receptors is associated with cgmp accumulation and that activation of h2-receptors is associated with cgmp accumulation. histamine increased both cgmp and cgmp levels with similar time course and concentration-re ... | 1978 | 32382 |
| effect of ph on the response of adrenal medulla to various agonists. | | 1978 | 32384 |
| [pharmaco-kinetic aspects of protein-binding (author's transl)]. | binding of drugs to plasma proteins will influence distribution and elimination especially if factors interfere altering the extent of binding. these factors are: dependence on species, age, h+-concentration, speed of i.v. injection or the interaction of another drug for instance. considering the quantitative aspects changes in binding to the plasma proteins will turn only then relevant to drug action if the extent of alteration is very large or the drugs are bound to a very high degree. but the ... | 1978 | 32427 |
| localization of the site of adenylylation of glutamine synthetase by electron microscopy of an enzyme-antibody complex. | antibodies to the nucleosidel,n(6)-ethenoadenosine have been used to localize the site of adenylylation of the glutamine synthetase [l-glutamate:ammonia ligase (adp-forming), ec 6.3.1.2] of escherichia coli. antibodies were induced in rabbits by injection of a bovine albumin-ethenoadenosine conjugate. the resulting antisera strongly bound ethenoadenosine, its 5'-nucleotide, or protein conjugates of the nucleoside; little or no crossreaction was seen to adenosine, amp, or the protein carrier. eth ... | 1978 | 32536 |
| selective labeling of serotonin receptors by d-[3h]lysergic acid diethylamide in calf caudate. | since it was known that d-lysergic acid diethylamide (lsd) affected catecholaminergic as well as serotoninergic neurons, the objective in this study was to enhance the selectivity of [3h]lsd binding to serotonin receptors in vitro by using crude homogenates of calf caudate. in the presence of a combination of 50 nm each of phentolamine (added to preclude the binding of [3h]lsd to alpha-adrenoceptors), apomorphine, and spiperone (added to preclude the binding of [3h]lsd to dopamine receptors), it ... | 1978 | 32537 |
| structure--inhibition relationships of various phenolic compounds towards mitochondrial respiration. | | 1978 | 32554 |
| biological and biochemical characteristics of fusobacterium necrophorum leukocidin. | the production of a leukocidal exotoxin by 18-hour dialysis cultures of fusobacterium necrophorum was detected in vitro by a cytotoxicity assay, using bovine leukocytes isolated from peripheral blood. biological characteristics were determined while maximizing the in vitro assay. biochemical characteristics of heat stability and stability to degradative enzymes were investigated and indicated that the leukocidin is protein in nature. the characteristics indicate that this leukocidin is different ... | 1978 | 32818 |
| [effects of the nature of energy in food and use of anabolic agents on the quality of veal]. | the effects of the nature of energy intake, of the slaughter weight and of the use of hormones on the quality of veal meat were studied. concerning the colour of meat, the use of hormones has no effect either on the pigment content or on the ultimate ph, but the nature of energy intake and the slaughter weight can affect these two parameters. with the increase of the slaughter weight and the substitution of lipids by starch in the diet, the probability to get a redder meat increases. for a given ... | 1978 | 32823 |
| injury to staphylococcus aureus during sausage fermentation. | staphylococcus aureus 196e added to a beef sausage containing starter culture and 0.5 to 2.0% glucose and incubated at 35 degrees c was unable to grow when plated on tryptic soy agar (tsa) containing 7.5% nacl. the injury, presumed to be due to the lactic acid produced during fermentation, was more pronounced at the lower concentrations of glucose (and lower acid levels). in the absence of glucose and/or starter culture, no injury was observed. when sausages containing s. aureus injured by ferme ... | 1978 | 32835 |
| pectinolytic enzymes of large rumen treponemes. | large spiral organisms isolated from the rumen of cattle produced and released into the external environment a complex of pectinolytic enzymes, consisting mainly of poly(1,4-alpha-d-galacturonide) lyase (ec 4.2.2.2, formerly ec 4.2.99.3), most active at ph 8.0 to 9.0, and another enzyme acting at ph below 7.0, probably a poly(1,4-alpha-d-galacturonide) glycanohydrolase (ec 3.2.1.15). the mixture of enzymes degraded polygalacturonate to saturated and unsaturated monogalacturonates as the end prod ... | 1979 | 32839 |
| electrostatic and conformational effects on the reaction of thiol groups of calf thymus histone h3 with 5,5'-dithiobis(2-nitrobenzoic acid). | | 1978 | 32845 |
| relaxation kinetic studies of coenzyme binding to glutamate dehydrogenase from beef liver. | | 1978 | 32881 |
| dna-bound proteases. partial characterization of proteolytic activities in commercial sources of dna. | the presence of variable amounts of protease in commercial sources of calf thymus dna has been established by means of three independent methods; electrophoresis, substrate labelling and microfluorometry. the microfluorometric method, which is particularly useful for initial velocity measurements, has been used to characterize this proteolytic activity, either bound to dna or solubilized by salt extraction. the proteolytic activity had a maximum at neutral ph and was not affected by nacl concent ... | 1978 | 32906 |
| enzymic synthesis of a new type of fucose-containing glycolipid with fucosyltransferase of rat ascites hepatoma cell, ah 7974f. | an alpha-fucosyltransferase activity has been demonstrated in rat ascites hepatoma ah 7974f cells catalyzing the transfer of l-fucose to asialo-gm1 prepared from bovine brain gm1 ganglioside to form a fucolipid in the presence of triton x-100. the radioactive fucolipid was shown to be fuc-(alpha1 leads to 2)-gal-(beta1 leads to 3)-galnac-(beta1 leads to 4)-gal-(beta1 leads to 4)-glc-ceramide from the following results. the radioactive product coincided with authentic blood group h-active fucolip ... | 1979 | 32911 |
| characterization of a soluble glycolipid galactosyltransferase which occurs in bovine milk. | bovine milk was found to contain, in soluble form, an enzyme which transfers galactose from udpgalactose to glucosylceramide. this enzyme was partially purified by the same procedure used to isolate the galactosyltransferase of lactose synthetase. the partially purified enzyme required detergents for activity, had a ph optimum of 7.2--7.3 and required mn2+. the apparent km calculated for glucosylceramide was 1.33 . 10(-4) m. with glucosylceramide as acceptor the product of the reaction was ident ... | 1979 | 32913 |
| 3-hydroxy-3-methylglutaryl coenzyme a reductase from avian liver. catalytic properties. | the catalytic properties of microsomal 3-hydroxy-3-methylglutaryl coenzyme a reductase from avian liver have been investigated. solubilized and highly purified reductase preparations were not cold labile, and enzymic activity remained unchanged following preincubation at 37 degrees c. the ph optimum was 6.8--7.0 and maximal catalytic activity was achieved with 2 mm dithiothreitol and 0.75 m kcl. the heat stability of the enzyme was studied and the addition of 0.75 m kcl, 0.8 mg/ml bovine serum a ... | 1979 | 32916 |
| the thermal unfolding of ribonuclease a. a 13c nmr study. | the 13c nuclear magnetic resonance (nmr) spectra of ribonuclease a over the ph range 1-7 and between 6 and 70 degrees c reveal many of the details of its reversible unfolding. although the unfolding may loosely be described as 'two-state', evidence is presented for intermediate unfolding stages at least 10 degrees c on either side of the main unfolding transition, particularly at low ph. the first residues to unfold are 17-24, in agreement with other results. the c-terminal region shows a steepe ... | 1979 | 32917 |
| effects of fluoride on in vitro calcification of tendon matrix. | ca2+ and pi uptake induced in vitro by a collagenous matrix derived from bovine tendon is inhibited by 1 x 10(-6) to 2 x 10(-5) m naf and stimulated by 2 x 10(-5) to 2 x 10(-3) m naf. fluoride uptake occurs only over the latter concentration range. the uptake of ca2+, pi, and f-1 progresses toward a limiting extent at which the molar ca/p and ca/f values are 1.6 to 1.7 and 4.5 to 5.7, respectively. although the matrix-bound mineral, previously formed in the absence of naf, readily undergoes diss ... | 1978 | 32959 |
| the effect of caffeine and quinine on calcium efflux and cyclic amp levels in bovine ileal smooth muscle. | | 1978 | 33012 |
| pharmacokinetics of sulfadimidine in normal and diarrheic calves. | pharmacokinetics of sulfadimidine after intravenous and oral administration of 50 mg per kg body weight were investigated in 2- to 22-day-old healthy and diarrheic calves. the data were processed in a digital computer. the values of absorption and elimination half-times after oral administration were 7.3 and 26 hr in healthy and 2.5 and 17.7 hr in diarrheic calves. it is evident from the results that the elimination of sulfadimidine in calves is very slow and characteristic of the species. in di ... | 1978 | 33025 |
| dissociation of light chains from cardiac myosin. | | 1978 | 33041 |
| affinity purification and properties of cathepsin-e-like acid proteinase from rat spleen. | a unique acid proteinase different from cathepsin d was purified from rat spleen by a method involving precipitation at ph 3.5, affinity chromatography on pepstatin-sepharose 4b and concanavalin a-sepharose 4b, chromatography on sephadex g-100 and deae-sephacel, and isoelectric focusing. a purification of 4200-fold over the homogenate was achieved and the yield was 11%. the purified enzyme appeared to be homogeneous on electrophoresis in polyacrylamide gels. the isoelectric point of the enzyme w ... | 1978 | 33048 |
| drug protein interaction: inhibition of glutamate dehydrogenase by psycoactive butyrophenones. | | 1978 | 33063 |
| scientific papers to be presented at the thirty-fifth annual meeting of the american fertility society, february 5 to 7, 1979. san francisco, california. | | 1978 | 33070 |
| catalytic role of the metal ion of carboxypeptidase a in ester hydrolysis. | the mechanism of action of bovine pancreatic carboxypeptidase. aalpha (peptidyl-l-amino acid hydrolase; ec 3.4.12.2) has been investigated by application of cryoenzymologic methods. kinetic studies of the hydrolysis of the specific ester substrate o-(trans-p-chlorocinnamoyl)-l-beta-phenyllactate have been carried out with both the native and the co2+-substituted enzyme in the 25 to --45 degrees c temperature range. in the --25 to --45 degrees c temperature range with enzyme in excess, a biphasic ... | 1979 | 33168 |
| in vitro phosphorylation of bovine adrenal tyrosine hydroxylase by adenosine 3':5'-monophosphate-dependent protein kinase. | we have studied the effects of adenosine 3':5'-monophosphate (camp)-dependent protein kinase on the phosphorylative and functional modification of bovine adrenal tyrosine hydroxylase. incubation of partially purified tyrosine hydroxylase with camp-dependent protein kinase in the presence of [gamma32p]atp and 5 micron camp led to a 3- to 5-fold activation of tyrosine hydroxylase and to incorporation of [32p]phosphate into protein. when tyrosine hydroxylase preparations activated by exposure to en ... | 1979 | 33170 |
| identification of the prosthetic group of urocanase. the mode of its reaction with sodium borohydride and of its photochemical reactivation. | urocanase from pseudomonas putida and from beef liver were isolated by modifying described procedures. both enzymes were inactivated and labeled on treatment with tritiated sodium borohydride and gave, upon subsequent hydrolysis, a radioactive acid. the previously reported identity of this acid as 2-hydroxybutanoic acid was disproved by several criteria. other hydroxy acids were also proved to be different from the radioactive acid derived from urocanase. a large portion of the radioactive mater ... | 1979 | 33176 |
| internal ph and state of atp in adrenergic chromaffin granules determined by 31p nuclear magnetic resonance spectroscopy. | | 1979 | 33185 |
| distinct alpha-noradrenergic receptors differentiated by binding and physiological relationships. | | 1979 | 33313 |
| d-amphetamine binding to brain lipid. | the interaction of d-amphetamine with several brain tissue components has been investigated. a brain lipid extract and a number of individual phospholipids were found to bind d-amphetamine when measured by means of a hexane-buffer partition coefficient technique. phosphatidylserine showed the greatest binding and phosphatidylethanolamine and dipalmitoyllecithin were also effective. phospholipid binding of d-amphetamine was shown to be related to fatty acid composition. cholesterol and gangliosid ... | 1978 | 33345 |
| evidence that catecholamine transport into chromaffin vesicles is coupled to vesicle membrane potential. | the effects of atp, mg(2+), and various agents on ph gradient, membrane potential, and catecholamine transport across membranes of intact bovine chromaffin vesicles were investigated. methylamine and thiocyanate (scn(-)) distributions across the vesicle membrane were used to estimate the h(+) concentration gradient and membrane potential, respectively. the h(+) concentration ratio (intravesiculanmedium) equals 16 when the medium ph is 6.9 and is unaltered by atp and mg(2+). in the absence of atp ... | 1978 | 33385 |
| intestinal uptake and transport of proteins in the adult rat. | the transport of immunoglobulin and ferritin across the intestinal mucosa of adult rats provides an excellent model for transcellular protein transport study. intestinal uptake and transcellular transport have been extensively studied in the neonatal rat, but not to such an extent in the adult rat. the transport of 125i labelled bovine immunoglobulin g and ferritin was studied in 100 days old rats using intestinally administered proteins. antigen was estimated in the tissues by reacting extracts ... | 1978 | 33390 |
| [different properties of fluorescein isothiocyanate isomers]. | | 1975 | 33419 |
| shifts in the conformation of serum albumin and their possible significance in normal catabolism. | | 1978 | 33444 |
| hydrolysis of long-chain fatty acyl-coa in homogenates of human blood platelets: the existence of a platelet palmitoyl-coa hydrolase. | the existence of a very active long-chain fatty acyl-coa hydrolase in homogenates of human blood platelets is reported. the highest activity was found with palmitoyl-coa as the substrate. palmitoyl-coa hydrolase activity was not found in intact platelets indicating that the enzyme is localized within the platelet membrane. no palmitoyl-coa hydrolase activity was found in fasting plasma. mg2+, mn2+, ca2+ and triton x-100 inhibited the palmitoyl-coa hydrolase activity. sulphydryl reagents had no e ... | 1978 | 33445 |
| studies of the tsh radioreceptor assay. | we have examined several variables in the reagents and procedures used in the tsh radioreceptor assay, the binding of iodinated tsh to its thyroidal receptor. we found that iodinated bovine tsh (s.a. 30 u/mg) was more effectively bound to receptor than iodinated human tsh (s.a. 7.3 u/mg). iodination of tsh was the bolton-hunter acylation method apparently prevented binding to tsh receptor. surgically removed human thyroid tissue specifically bound 10.3 +/- 1.0 (mean +/- sem) of added [125i]tsh, ... | 1979 | 33516 |
| cow's milk allergy: pathogenesis, manifestations, diagnosis and management. | | 1978 | 33545 |
| studies on ionized calcium in serum and plasma from normal cows. its relation to total serum calcium and the effects of sample storing. | | 1978 | 33546 |
| hysteretic kinetic behavior of beef liver pyruvate carboxylase. | | 1978 | 33599 |
| subcellular localization of gamma-glutamyltransferase in calf thymocytes. | the subcellular localization of gamma-glutamyltransferase in calf thymocytes was investigated and compared with that of alkaline phosphodiesterase i, alkaline nitrophenyl phosphatase, succinate-tetrazolium oxidoreductase (succinate-int reductase) and lactate dehydrogenase after two different methods of cell disruption and differential centrifugation. most of the activity was recovered in the crude membrane fractions (43.0%), but significant amounts co-pelleted with the large-granule (mitochondri ... | 1978 | 33659 |
| ovalbumin digestion by human pepsins 1, 3 and 5. | 1. of the three major human pepsins, pepsin 1 has greater proteolytic activity towards ovalbumin than has pepsin 3. pepsin 5 has low activity towards this substrate. 2. proteolytic ph-activity curves show only on ph maximum, about ph 1.4 for pepsin 1, ph 1.4--1.5 for pepsin 3 and ph 1.2--1.4 for pepsin 5. the curve for pepsin 3 has a shoulder between ph 2.4 and 3.4. 3. the rate of digestion of ovalbumin by pepsin 1 is approximately three times slower than are those of bovine haemoglobin or human ... | 1978 | 33668 |
| conversion of high molecular weight crf (corticotropin-releasing factor) or prf (prolactin-releasing factor) into lower molecular weight forms by boiling at low ph. | | 1978 | 33676 |