| malaria pathogenesis. | in the mosquito-human life cycle, the six species of malaria parasites infecting humans (plasmodium falciparum, plasmodium vivax, plasmodium ovale wallickeri, plasmodium ovale curtisi, plasmodium malariae, and plasmodium knowlesi) undergo 10 or more morphological states, replicate from single to 10,000+ cells, and vary in total population from one to many more than 10(6) organisms. in the human host, only a small number of these morphological stages lead to clinical disease and the vast majority ... | 2017 | 28533315 |
| evaluation of the utility value of three diagnostic methods in the detection of malaria parasites in endemic area. | malaria is a debilitating disease with high morbidity and mortality in africa, commonly caused by different species of the genus plasmodium in humans. misdiagnosis is a major challenge in endemic areas because of other disease complications and technical expertise of the medical laboratory staff. microscopic method using giemsa-stained blood film has been the mainstay of diagnosis of malaria. however, since 1993 when rapid diagnostic test (rdt) kits were introduced, they have proved to be effect ... | 2017 | 28477621 |
| nested multiplex pcr for identification and detection of human plasmodium species including plasmodium knowlesi. | to develop a new technique for diagnosis of plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of plasmodium causing human malaria. | 2017 | 28442114 |
| prevalence of plasmodium falciparum and non-p. falciparum infections in a highland district in ghana, and the influence of hiv and sickle cell disease. | in the past two decades, there has been a reported decline in malaria in ghana and the rest of the world; yet it remains the number one cause of mortality and morbidity. human immuno-deficiency virus (hiv) and sickle cell disease (scd) share a common geographical space with malaria in sub-saharan africa and an interaction between these three conditions has been suggested. this study determined the plasmodium falciparum and non-p. falciparum status of symptomatic and non-symptomatic residents of ... | 2017 | 28438159 |
| metal-dna interactions improve signal in high-resolution melting of dna for species differentiation of plasmodium parasite. | the success of high-resolution melting (hrm) analysis for distinguishing similar dnas with minor base mismatch differences is limited. here, metal-mediated structural change in dna has been exploited to amplify hrm signals leading to differentiation of target dnas in an orthologous gene corresponding to four plasmodium species. conserved 26-mer ssdnas from ldh gene of the four plasmodium species were employed as targets. a capture probe (cp) that is fully complementary to the plasmodium falcipar ... | 2017 | 28421327 |
| plasmodium knowlesi malaria in a traveller returning from the philippines to italy, 2016. | plasmodium knowlesi is a simian parasite responsible for most human cases of malaria in malaysian borneo. a timely recognition of infection is crucial because of the risk of severe disease due to the rapid increase in parasitemia. we report a case of p. knowlesi infection in a traveller who developed fever and thrombocytopenia after returning from the philippines in 2016. rapid antigen test was negative, microscopy examination showed parasites similar to plasmodium malariae, with a parasite coun ... | 2017 | 28675245 |
| genomic characterization of recrudescent plasmodium malariae after treatment with artemether/lumefantrine. | plasmodium malariae is the only human malaria parasite species with a 72-hour intraerythrocytic cycle and the ability to persist in the host for life. we present a case of a p. malariae infection with clinical recrudescence after directly observed administration of artemether/lumefantrine. by using whole-genome sequencing, we show that the initial infection was polyclonal and the recrudescent isolate was a single clone present at low density in the initial infection. haplotypic analysis of the c ... | 2017 | 28430103 |