| direct ethanol production from glucose, xylose and sugarcane bagasse by the corn endophytic fungi fusarium verticillioides and acremonium zeae. | production of ethanol with two corn endophytic fungi, fusarium verticillioides and acremonium zeae, was studied. the yield of ethanol from glucose, xylose and a mixture of both sugars were 0.47, 0.46 and 0.50g/g ethanol/sugar for f. verticillioides and 0.37, 0.39 and 0.48g/g ethanol/sugar for a. zeae. both fungi were able to co-ferment glucose and xylose. ethanol production from 40g/l of pre-treated sugarcane bagasse was 4.6 and 3.9g/l for f. verticillioides and a. zeae, respectively, yielding 0 ... | 2013 | 23942376 |
| development and nitrate reductase activity of sugarcane inoculated with five diazotrophic strains. | diazotrophs are able to stimulate plant growth. this study aimed at evaluating the effect of inoculation of five diazotrophic strains on growth promotion and nitrate reductase (nr, ec 1.7.1.1) activity in sugarcane. an experiment was carried out from three stages of cultivation: sprouting, tubes, and in hydroponics. on the first two stages, seven treatments were adopted: uninoculated control; mixed inoculation with five strains; and individual inoculation with gluconacetobacter diazotrophicus (g ... | 2017 | 28321482 |
| the decrease in the population of gluconacetobacter diazotrophicus in sugarcane after nitrogen fertilization is related to plant physiology in split root experiments. | it has been established that a decrease in the population of gluconacetobacter diazotrophicus associated with sugarcane occurs after nitrogen fertilization. this fact could be due to a direct influence of nh(4)no(3) on bacterial cells or to changes in plant physiology after fertilizer addition, affecting bacterial establishment. in this work, we observed that survival of g. diazotrophicus was directly influenced when 44.8mm of nh(4)no(3) (640mgn/plant) was used for in vitro experiments. furtherm ... | 2016 | 26652262 |
| tn5 insertion in the tonb gene promoter affects iron-related phenotypes and increases extracellular siderophore levels in gluconacetobacter diazotrophicus. | tonb-dependent receptors in concert with the tonb-exbb-exbd protein complex are responsible for the uptake of iron and substances such as vitamin b12 in several bacterial species. in this study, tn5 mutagenesis of the sugarcane endophytic bacterium gluconacetobacter diazotrophicus led to the isolation of a mutant with a single tn5-insertion in the promoter region of a tonb gene ortholog. this mutant, named gdiaa31, displayed a reduced growth rate and a lack of response to iron availability when ... | 2015 | 25323530 |
| identification and characterization of an iron abc transporter operon in gluconacetobacter diazotrophicus pal 5. | gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane. we have cloned and sequenced the genes coding for the components of the iron abc-type acquisition system of g. diazotrophicus. sequence analysis revealed three orfs, (feua, feub, and feuc) organized as an operon and encoding polypeptides of 346 (38 kda), 342 (34.2 kda), and 240 (26 kda) amino acids, respectively. the deduced translation products of the feu operon showed similarity with a periplasmic solut ... | 2013 | 23624722 |
| identification and validation of reference genes to study the gene expression in gluconacetobacter diazotrophicus grown in different carbon sources using rt-qpcr. | gluconacetobacter diazotrophicus strain pal5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. currently, g. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens. reverse transcription fol ... | 2012 | 22814372 |
| identification of n-acyl homoserine lactones produced by gluconacetobacter diazotrophicus pal5 cultured in complex and synthetic media. | the endophytic diazotrophic gluconacetobacter diazotrophicus pal5 was originally isolated from sugarcane (saccharum officinarum). the biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. the recent genomic sequence of pal5 unveiled the presence of a quorum sensing (qs) system. qs are regulatory mechanisms that, through the production of signal molecules or autoinduce ... | 2012 | 22350020 |
| polyphasic characterization of gluconacetobacter diazotrophicus isolates obtained from different sugarcane varieties. | a polyphasic approach was applied to characterize 35 g. diazotrophicus isolates obtained from sugarcane varieties cultivated in brazil. the isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ardra and risa-rflp techniques). variability among the isolates was observed in relation to the carbon source use preference. glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. the results of the ... | 2008 | 24031296 |
| genomic comparison of the endophyte herbaspirillum seropedicae smr1 and the phytopathogen herbaspirillum rubrisubalbicans m1 by suppressive subtractive hybridization and partial genome sequencing. | herbaspirillum rubrisubalbicans m1 causes the mottled stripe disease in sugarcane cv. b-4362. inoculation of this cultivar with herbaspirillum seropedicae smr1 does not produce disease symptoms. a comparison of the genomic sequences of these closely related species may permit a better understanding of contrasting phenotype such as endophytic association and pathogenic life style. to achieve this goal, we constructed suppressive subtractive hybridization (ssh) libraries to identify dna fragments ... | 2012 | 22268687 |
| seasonal phenology and management of tomarus subtropicus (coleoptera: scarabaeidae) in st. augustinegrass. | the grub tomarus subtropicus blatchley (coleoptera: scarabaeidae) is one of the most destructive pests of turfgrass and sugarcane (saccharum spp.) in southern florida, but its life cycle has only been described in sugarcane production. preventive insecticides applied against t. subtropicus in turfgrass when adult activity and oviposition were expected in sugarcane have necessitated subsequent curative treatments. thus, the seasonal activity of t. subtropicus was monitored using uv blacklight tra ... | 2008 | 19133466 |
| iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences. | iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ... | 2017 | 27632283 |
| biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation. | hydrogen production from sugarcane bagasse (scb) by integrating dark-fermentation by enterobacter aerogenes mtcc 2822 and photo-fermentation by rhodopseudomonas bhu 01 was investigated. the scb was hydrolysed by sulphuric acid and the hydrolysate detoxified by passing through adsorbent resin column (amberlite xad-4) to remove the inhibitory furfural, and subjected to dark-fermentation. the cellulosic residue from acid hydrolysis was hydrolysed by the new isolate cellulomonas fimi to release suga ... | 2014 | 24291314 |
| engineered enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production. | sugarcane molasses is considered to be a good carbon source for biorefinery due to its high sugar content and low price. sucrose occupies more than half of the sugar in the molasses. enterobacter aerogenes is a good host strain for 2,3-butanediol production, but its utilization of sucrose is not very efficient. to improve sucrose utilization in e. aerogenes, a sucrose regulator (scrr) was disrupted from the genomic dna. the deletion mutation increased the sucrose consumption rate significantly w ... | 2013 | 23644066 |
| efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered klebsiella oxytoca in mineral salts medium. | klebsiella oxytoca kms005 (∆adhe∆acka-pta∆ldha) was metabolically engineered to improve 2,3-butanediol (bdo) yield. elimination of alcohol dehydrogenase e (adhe), acetate kinase a-phosphotransacetylase (acka-pta), and lactate dehydrogenase a (ldha) enzymes allowed bdo production as a primary pathway for nadh re-oxidation, and significantly reduced by-products. kms005 was screened for the efficient glucose utilization by metabolic evolution. kms005-73t improved bdo production at a concentration o ... | 2015 | 25895450 |
| complete genome sequence of endophytic nitrogen-fixing klebsiella variicola strain dx120e. | klebsiella variicola strain dx120e (=cgmcc 1.14935) is an endophytic nitrogen-fixing bacterium isolated from sugarcane crops grown in guangxi, china and promotes sugarcane growth. here we summarize the features of the strain dx120e and describe its complete genome sequence. the genome contains one circular chromosome and two plasmids, and contains 5,718,434 nucleotides with 57.1% gc content, 5,172 protein-coding genes, 25 rrna genes, 87 trna genes, 7 ncrna genes, 25 pseudo genes, and 2 crispr re ... | 2015 | 26203334 |
| [growth-promoting effect of inoculating klebsiella variicola dx120e on different sugarcane cultivars]. | in order to investigate the growth promoting effect of inoculating klebsiella variicola dx120e, a bacterial strain with high activity of associative nitrogen fixation, on sugarcane, the strain was inoculated through roots into the pathogen free micropropagated seedlings of two sugarcane cultivars b8 and gt21. the bacterial numbers colonized in sugarcane plants, the activities of the key enzymes for nitrogen metabolism, the nitrate concentration and nutrient uptake were analyzed. the results indi ... | 2014 | 25345062 |
| sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept. | the environmental impact of agricultural waste from the processing of food and feed crops is an increasing concern worldwide. concerted efforts are underway to develop sustainable practices for the disposal of residues from the processing of such crops as coffee, sugarcane, or corn. coffee is crucial to the economies of many countries because its cultivation, processing, trading, and marketing provide employment for millions of people. in coffee-producing countries, improved technology for treat ... | 2014 | 25204861 |
| draft genome sequence of kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate. | we determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported k. marxianus strains. the genome described here is composed of 11,165,408 bases and has 4,943 protein-coding genes. | 2014 | 25059876 |
| modeling the microbial growth and temperature profile in a fixed-bed bioreactor. | aiming to scale up and apply control and optimization strategies, currently is required the development of accurate plant models to forecast the process nonlinear dynamics. in this work, a mathematical model to predict the growth of the kluyveromyces marxianus and temperature profile in a fixed-bed bioreactor for solid-state fermentation using sugarcane bagasse as substrate was built up. a parameter estimation technique was performed to fit the mathematical model to the experimental data. the es ... | 2014 | 24658796 |
| optimization, kinetics, and modeling of inulinase production by k. marxianus var. marxianus. | pressmud, a by-product from the sugarcane industry, was used as a carbon source for the production of inulinase in solid-state fermentation (ssf). statistical experimental designs were employed to screen the nutrients and optimize the media composition for the production of inulinase by kluyveromyces marxianus var. marxianus. eighteen various nutrients were selected for preliminary screening of production medium component by plackett-burman design (pbd) technique. five nutrients were found to be ... | 2014 | 24274017 |
| high-temperature ethanol fermentation by immobilized coculture of kluyveromyces marxianus and saccharomyces cerevisiae. | suspended and immobilized cocultures of the thermotolerant yeast, kluyveromyces marxianus dmku 3-1042 and the mesophilic flocculent yeast, saccharomyces cerevisiae m30 were studied for their abilities to improve production and stability of ethanol fermentation. sugarcane juice and blackstrap molasses, at initial sugar concentrations of 220 g/l, were used as carbon sources. the results indicated that the coculture system could improve ethanol production from both sugarcane juice and blackstrap mo ... | 2012 | 22608995 |
| the influence of presaccharification, fermentation temperature and yeast strain on ethanol production from sugarcane bagasse. | ethanol can be produced from cellulosic biomass in a process known as simultaneous saccharification and fermentation (ssf). the presence of yeast together with the cellulolytic enzyme complex reduces the accumulation of sugars within the reactor, increasing the ethanol yield and saccharification rate. this paper reports the isolation of saccharomyces cerevisiae lbm-1, a strain capable of growth at 42 °c. in addition, s. cerevisiae lbm-1 and kluyveromyces marxianus ufv-3 were able to ferment suga ... | 2012 | 22285296 |
| saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production. | the bioethanol production system used in brazil is based on the fermentation of sucrose from sugarcane feedstock by highly adapted strains of the yeast saccharomyces cerevisiae. bacterial contaminants present in the distillery environment often produce yeast-bacteria cellular co-aggregation particles that resemble yeast-yeast cell adhesion (flocculation). the formation of such particles is undesirable because it slows the fermentation kinetics and reduces the overall bioethanol yield. | 2015 | 25633848 |
| homo- and heterofermentative lactobacilli differently affect sugarcane-based fuel ethanol fermentation. | bacterial contamination during industrial yeast fermentation has serious economic consequences for fuel ethanol producers. in addition to deviating carbon away from ethanol formation, bacterial cells and their metabolites often have a detrimental effect on yeast fermentative performance. the bacterial contaminants are commonly lactic acid bacteria (lab), comprising both homo- and heterofermentative strains. we have studied the effects of these two different types of bacteria upon yeast fermentat ... | 2014 | 24198118 |
| meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation, aerobic stability, and the performance of dairy cows. | forages are usually inoculated with homofermentative and facultative heterofermentative lactic acid bacteria (lab) to enhance lactic acid fermentation of forages, but effects of such inoculants on silage quality and the performance of dairy cows are unclear. therefore, we conducted a meta-analysis to examine the effects of lab inoculation on silage quality and preservation and the performance of dairy cows. a second objective was to examine the factors affecting the response to silage inoculatio ... | 2017 | 28342607 |
| fermentative profile and bacterial diversity of corn silages inoculated with new tropical lactic acid bacteria. | this study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria (lab) isolated from sugarcane grown in a brazil on the quality of corn silage. | 2016 | 26496890 |
| efficient mannitol production by wild-type lactobacillus reuteri crl 1101 is attained at constant ph using a simplified culture medium. | mannitol is a natural polyol with multiple industrial applications. in this work, mannitol production by lactobacillus reuteri crl 1101 was studied at free- and controlled-ph (6.0-4.8) fermentations using a simplified culture medium containing yeast and beef extracts and sugarcane molasses. the activity of mannitol 2-dehydrogenase (mdh), the enzyme responsible for mannitol synthesis, was determined. the effect of the initial biomass concentration was further studied. mannitol production (41.5 ± ... | 2015 | 26084891 |
| lactobacillus reuteri crl 1101 highly produces mannitol from sugarcane molasses as carbon source. | mannitol is a natural polyol extensively used in the food industry as low-calorie sugar being applicable for diabetic food products. we aimed to evaluate mannitol production by lactobacillus reuteri crl 1101 using sugarcane molasses as low-cost energy source. mannitol formation was studied in free-ph batch cultures using 3-10% (w/v) molasses concentrations at 37 °c and 30 °c under static and agitated conditions during 48 h. l. reuteri crl 1101 grew well in all assayed media and heterofermentativ ... | 2012 | 22350320 |
| microencapsulation of alginate-immobilized bagasse with lactobacillus rhamnosus nrrl 442: enhancement of survivability and thermotolerance. | the aim of this research was to enhance the survivability of lactobacillus rhamnosus nrrl 442 against heat exposure via a combination of immobilization and microencapsulation processes using sugarcane bagasse (sb) and sodium alginate (naa), respectively. the microcapsules were synthesized using different alginate concentration of 1, 2 and 3% and naa:sb ratio of 1:0, 1:1 and 1:1.5. this beneficial step of probiotic immobilization before microencapsulation significantly enhanced microencapsulation ... | 2015 | 25563958 |
| hantavirus pulmonary syndrome in santa cruz, bolivia: outbreak investigation and antibody prevalence study. | we report the results of an investigation of a small outbreak of hantavirus pulmonary syndrome in 2002 in the department of santa cruz, bolivia, where the disease had not previously been reported. two cases were initially reported. the first case was a physician infected with laguna negra virus during a weekend visit to his ranch. four other persons living on the ranch were igm antibody-positive, two of whom were symptomatic for mild hantavirus pulmonary syndrome. the second case was a migrant s ... | 2012 | 23094116 |
| draft genome sequence of leifsonia xyli subsp. xyli strain gdw1. | here, we report the draft genome sequence of leifsonia xyli subsp. xyli strain gdw1, isolated from the stem of badila sugarcane located at the guangdong key laboratory for crops genetic improvement (guanzhou, china), that causes ratoon stunting disease of sugarcane. the de novo genome of leifsonia xyli subsp. xyli was assembled with 48 scaffolds and a g+c content of 67.68%, and contained 2.6 mb bp and 2,838 coding sequences. | 2016 | 27795270 |
| rapid and quantitative detection of leifsonia xyli subsp. xyli in sugarcane stalk juice using a real-time fluorescent (taqman) pcr assay. | ratoon stunting disease (rsd) of sugarcane, one of the most important diseases seriously affecting the productivity of sugarcane crops, was caused by the bacterial agent leifsonia xyli subsp. xyli (lxx). a taqman probe-based real-time quantitative polymerase chain reaction (qpcr) assay was established in this study for the quantification of lxx detection in sugarcane stalk juice. a pair of pcr primers (pat1-qf/pat1-qr) and a fluorogenic probe (pat1-qp) targeting the part1 gene of lxx were used f ... | 2016 | 27725937 |
| a rapid and visual loop-mediated isothermal amplification assay to detect leifsonia xyli subsp. xyli targeting a transposase gene. | leifsonia xyli subsp. xyli (lxx), causal organism of ratoon stunt (rsd), does not produce any reliable internal or external symptoms on sugarcane. its detection on a large scale is solely based on microscopic and serological methods. these methods require well-equipped laboratories, are time consuming and are not feasible for near-field detection of lxx. in this study, we developed a loop-mediated isothermal amplification (lamp) assay for rapid and sensitive detection of lxx without the use of s ... | 2014 | 25201631 |
| complete genome sequence of leifsonia xyli subsp. cynodontis strain dsm46306, a gram-positive bacterial pathogen of grasses. | we announce the complete genome sequence of leifsonia xyli subsp. cynodontis, a vascular pathogen of bermuda grass. the species also comprises leifsonia xyli subsp. xyli, a sugarcane pathogen. since these two subspecies have genome sequences available, a comparative analysis will contribute to our understanding of the differences in their biology and host specificity. | 2013 | 24201198 |
| development of loop-mediated isothermal amplification for detection of leifsonia xyli subsp. xyli in sugarcane. | ratoon stunt, caused by the xylem-limited coryneform bacterium leifsonia xyli subsp. xyli (lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. based on loop-mediated isothermal amplification (lamp), we developed a method for detecting lxx. the major advantages of the lamp method are visual judgment by color and time saving with only 60 min for identification of lxx and without the need for costly pcr apparatus and gel scanner. in the present study, positive and ne ... | 2013 | 23710444 |
| diverse exopolysaccharide producing bacteria isolated from milled sugarcane: implications for cane spoilage and sucrose yield. | bacterial deterioration of sugarcane during harvesting and processing is correlated with significant loss of sucrose yield and the accumulation of bacterial polysaccharides. dextran, a homoglucan produced by leuconostoc mesenteroides, has been cited as the primary polysaccharide associated with sugarcane deterioration. a culture-based approach was used to isolate extracellular polysaccharide (eps) producing bacterial strains from milled sugarcane stalks. ribosomal rna sequencing analysis grouped ... | 2015 | 26710215 |
| further characterization of maize chlorotic mottle virus and its synergistic interaction with sugarcane mosaic virus in maize. | maize chlorotic mottle virus (mcmv) was first reported in maize in china in 2009. in this study we further analyzed the epidemiology of mcmv and corn lethal necrosis disease (clnd) in china. we determined that clnd observed in china was caused by co-infection of mcmv and sugarcane mosaic virus (scmv). phylogenetic analysis using four full-length mcmv cdna sequences obtained in this study and the available mcmv sequences retrieved from genbank indicated that chinese mcmv isolates were derived fro ... | 2017 | 28059116 |
| synergistic infection of two viruses mcmv and scmv increases the accumulations of both mcmv and mcmv-derived sirnas in maize. | the co-infection of maize chlorotic mottle virus (mcmv) and sugarcane mosaic virus (scmv) can cause maize lethal necrosis. however, the mechanism underlying the synergistic interaction between these two viruses remains elusive. in this study, we found that the co-infection of mcmv and scmv increased the accumulation of mcmv. moreover, the profiles of virus-derived sirnas (vsirnas) from mcmv and scmv in single- and co-infected maize plants were obtained by high-throughput sequencing. our data sho ... | 2016 | 26864602 |
| genome-wide association and genomic prediction of resistance to maize lethal necrosis disease in tropical maize germplasm. | genome-wide association analysis in tropical and subtropical maize germplasm revealed that mlnd resistance is influenced by multiple genomic regions with small to medium effects. the maize lethal necrosis disease (mlnd) caused by synergistic interaction of maize chlorotic mottle virus and sugarcane mosaic virus, and has emerged as a serious threat to maize production in eastern africa since 2011. our objective was to gain insights into the genetic architecture underlying the resistance to mlnd b ... | 2015 | 26152570 |
| maize lethal necrosis (mln), an emerging threat to maize-based food security in sub-saharan africa. | in sub-saharan africa, maize is a staple food and key determinant of food security for smallholder farming communities. pest and disease outbreaks are key constraints to maize productivity. in september 2011, a serious disease outbreak, later diagnosed as maize lethal necrosis (mln), was reported on maize in kenya. the disease has since been confirmed in rwanda and the democratic republic of congo, and similar symptoms have been reported in tanzania, uganda, south sudan, and ethiopia. in 2012, y ... | 2015 | 25822185 |
| recombination analysis of maize dwarf mosaic virus (mdmv) in the sugarcane mosaic virus (scmv) subgroup of potyviruses. | recombination among rna viruses is a natural phenomenon that appears to have played a significant role in the species development and the evolution of many strains. it also has particular significance for the risk assessment of plants which have been genetically modified for disease resistance by incorporating viral sequences into their genomes. however, the exact recombination events taking place in viral genomes are not investigated in detail for many virus groups. in this analysis, different ... | 2015 | 25392089 |
| genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line. | novel and previously known resistance loci for six phylogenetically diverse viruses were tightly clustered on chromosomes 2, 3, 6 and 10 in the multiply virus-resistant maize inbred line, oh1vi. virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. genetic resistance to different viruses has been characterized in maize populations in diverse environments using different screening techniques, and resistance loci hav ... | 2014 | 24500307 |
| a high degree of african streak virus diversity within nigerian maize fields includes a new mastrevirus from axonopus compressus. | the a-strain of maize streak virus (msv-a; genus mastrevirus, family geminiviridae), the causal agent of maize streak disease, places a major constraint on maize production throughout sub-saharan africa. in west-african countries such as nigeria, where maize is not cultivated year-round, this msv strain is forced to overwinter in non-maize hosts. in order to both identify uncultivated grasses that might harbour msv-a during the winter season and further characterise the diversity of related maiz ... | 2014 | 24796552 |
| the assessment of epiphytic yeast diversity in sugarcane phyllosphere in thailand by culture-independent method. | the diversity of epiphytic yeasts from sugarcane (saccharum officinarum linn.) phyllospheres in thailand was investigated by culture-independent method based on the analysis of the d1/d2 domains of the large subunit rrna gene sequences. forty-five samples of sugarcane leaf were collected randomly from ten provinces in thailand. a total of 1342 clones were obtained from 45 clone libraries. 426 clones (31.7 %) were closely related to yeast strains in the genbank database, and they were clustered i ... | 2015 | 26615738 |
| organic loading rate impact on biohydrogen production and microbial communities at anaerobic fluidized thermophilic bed reactors treating sugarcane stillage. | this study aimed to evaluate the effect of high organic loading rates (olr) (60.0-480.00 kg cod m(-3)d(-1)) on biohydrogen production at 55°c, from sugarcane stillage for 15,000 and 20,000 mg codl(-1), in two anaerobic fluidized bed reactors (afbr1 and afbr2). it was obtained, for h2 yield and content, a decreasing trend by increasing the olr. the maximum h2 yield was observed in afbr1 (2.23 mmol g cod added(-1)). the volumetric h2 production was proportionally related to the applied hydraulic r ... | 2014 | 24632626 |
| detection and quantification of root-knot nematode (meloidogyne javanica), lesion nematode (pratylenchus zeae) and dagger nematode (xiphinema elongatum) parasites of sugarcane using real-time pcr. | a number of different plant parasitic nematode species are found associated with sugarcane in south africa. of these, the root-knot nematode (meloidogyne javanica), the lesion nematode (pratylenchus zeae) and the dagger nematode (xiphinema elongatum) are potentially the most damaging pests. identification and enumeration of the number of these nematodes are necessary for providing advice to farmers as well as studying the effects of various treatments in field and glasshouse trials. we report on ... | 2008 | 18378423 |
| species diversity and population dynamics of entomopathogenic fungal species in the genus metarhizium-a spatiotemporal study. | we studied the species diversity and population genetic structure of isolates of fungi from the entomopathogenic genus metarhizium that had been isolated from sugarcane crops and surrounding grass. soil and leaf samples were taken on four sampling occasions over 13 months (october 2014-october 2015). isolations were made using the galleria mellonella baiting method and selective media. phylogenetic placement of isolates was done by sequencing a fragment of the 5' of the elongation factor 1-α gen ... | 2017 | 28124726 |
| belowground ecology of scarabs feeding on grass roots: current knowledge and future directions for management in australasia. | many scarab beetles spend the majority of their lives belowground as larvae, feeding on grass roots. many of these larvae are significant pests, causing damage to crops and grasslands. damage by larvae of the greyback cane beetle (dermolepida albohirtum), for example, can cause financial losses of up to au$40 million annually to the australian sugarcane industry. we review the ecology of some scarab larvae in australasia, focusing on three subfamilies; dynastinae, rutelinae, and melolonthinae, c ... | 2016 | 27047506 |
| isolation and characterization of xylitol-producing yeasts from the gut of colleopteran insects. | a total of 35 yeasts were isolated from the gut of beetles collected from hyderabad city, india. twenty of these yeasts utilized xylose as a sole carbon source but only 12 of these converted xylose to xylitol. the ability to convert xylose to xylitol varied among the isolates and ranged from 0.12 to 0.58 g/g xylose. based on the phenotypic characteristics and phylogenetic analysis of the d1/d2 domain sequence of 26s rrna gene, these isolates were identified as members of pichia, candida, issatch ... | 2007 | 17710483 |
| how native yeasts may influence the chemical profile of the brazilian spirit, cachaça? | cachaça is the typical brazilian spirit, obtained by distillation of fermented sugarcane must, whose production is closely related to regional heritage and geographical traits. in this work, we investigate the influence of different autochthonous yeast species, in comparison to other commercial strains, on the chemical profile of cachaça. fermentations were performed with four different wild species, a mixed starter culture with native yeasts, and two commercial strains. procedures were carried ... | 2017 | 28290322 |
| a strain of meyerozyma guilliermondii isolated from sugarcane juice is able to grow and ferment pentoses in synthetic and bagasse hydrolysate media. | the search for new microbial strains that are able to withstand inhibitors released from hemicellulosic hydrolysis and are also still able to convert sugars in ethanol/xylitol is highly desirable. a yeast strain isolated from sugarcane juice and identified as meyerozyma guilliermondii was evaluated for the ability to grow and ferment pentoses in synthetic media and in sugarcane bagasse hydrolysate. the yeast grew in xylose, arabinose and glucose at the same rate at an initial medium ph of 5.5. a ... | 2016 | 27038950 |
| saccharification of ozonated sugarcane bagasse using enzymes from myceliophthora thermophila jcp 1-4 for sugars release and ethanol production. | the saccharification of ozonated sugarcane bagasse (scb) by enzymes from myceliophthora thermophila jcp 1-4 was studied. fungal enzymes provided slightly higher sugar release than commercial enzymes, working at 50°c. sugar release increased with temperature increase. kinetic studies showed remarkable glucose release (4.99 g/l, 3%w/w dry matter) at 60°c, 8 h of hydrolysis, using an enzyme load of 10 fpu (filter paper unit). fpase and β-glucosidase activities increased during saccharification (284 ... | 2016 | 26773948 |
| thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification. | to obtain new cellulases and xylanases from thermophilic fungi; evaluate their potential for sugarcane bagasse saccharification. | 2015 | 25644433 |
| filamentous fungi and media for cellulase production in solid state cultures. | cellulase production was evaluated in two reference strains (t. reesei rut-c30 and t. reesei qm9414), two strains isolated from a sugarcane cultivation area (trichoderma sp. ipt778 and t. harzianum rifai ipt821) and one strain isolated in a program for biodiversity preservation in são paulo state (myceliophthora thermophila m77). solid state cultures were performed using sugarcane bagasse (c), wheat bran (w) and/or soybean bran (s). the highest fpa was 10.6 u/gdm for m77 in sc (10:90) at 80% moi ... | 2014 | 24948946 |
| deletion of ph regulator pac-3 affects cellulase and xylanase activity during sugarcane bagasse degradation by neurospora crassa. | microorganisms play a vital role in bioethanol production whose usage as fuel energy is increasing worldwide. the filamentous fungus neurospora crassa synthesize and secrete the major enzymes involved in plant cell wall deconstruction. the production of cellulases and hemicellulases is known to be affected by the environmental ph; however, the regulatory mechanisms of this process are still poorly understood. in this study, we investigated the role of the ph regulator pac-3 in n. crassa during t ... | 2017 | 28107376 |
| molecular and functional analyses of incompatibility genes at het-6 in a population of neurospora crassa. | two closely linked genes, un-24 and het-6, associated with the het-6 heterokaryon incompatibility functional haplotype were examined in 40 neurospora crassa strains from a louisiana sugarcane field. partial diploid analyses were used to determine that half of the strains were functionally oak ridge (or) and half were non-or and indistinguishable from the standard panama (pa) form. pcr-based markers were developed to identify polymorphisms within both un-24 and het-6. two common forms of each gen ... | 2000 | 11035941 |
| a glucuronoxylan-specific xylanase from a new paenibacillus favisporus strain isolated from tropical soil of brazil. | a new xylanolytic strain, paenibacillus favisporus cc02-n2, was isolated from sugarcane plantation fields in brazil. the strain had a xylan-degrading system with multiple enzymes, one of which, xylanase xyn30a, was identified and characterized. the enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (gh30). xyn30a shows high activity on glucuronoxylans, with a vmax of 267.2 u mg⁻¹, a km of 4.0 mg/ml, and a kcat of 13,333 min⁻¹ on beechwood xylan, but it does not ... | 2014 | 26419457 |
| comprehensive utilization of glycerol from sugarcane bagasse pretreatment to fermentation. | in this study, the effects of glycerol pretreatment on subsequent glycerol fermentation and biomass fast pyrolysis were investigated. the liquid fraction from the pretreatment process was evaluated to be feasible for fermentation by paenibacillus polymyxa and could be an economic substrate. the pretreated biomass was further utilized to obtain levoglucosan by fast pyrolysis. the pretreated sugarcane bagasse exhibited significantly higher levoglucosan yield (47.70%) than that of un-pretreated sam ... | 2015 | 26241838 |
| optimization and characterization of pha from isolate pannonibacter phragmitetus erc8 using glycerol waste. | polyhydroxyalkanoates (phas) have been considered as a good alternative for petrochemical based polymers due to its biodegradability. however, a high production cost limits their acceptance in industries. in present work, efforts have been made to optimize the production of pha by pannonibacter phragmitetus erc8 using glycerol waste as a sole carbon source, with enhanced polymer production in a cost effective way. to check the possibility of growth and polymer accumulation potential of p. phragm ... | 2016 | 26851207 |
| isolation of pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion. | each year, approximately 170 million metric tons of chemical fertilizer are consumed by global agriculture. furthermore, some chemical fertilizers contain toxic by-products and their long-term use may contaminate groundwater, lakes, and rivers. the use of plant growth-promoting bacteria may be a cost-effective strategy for partially replacing conventional chemical fertilizers, and may become an integrated plant nutrient solution for sustainable crop production. the main direct bacteria-activated ... | 2015 | 26634494 |
| optimization of lipids production by cryptococcus laurentii 11 using cheese whey with molasses. | this study aimed the optimization of culture condition and composition for production of cryptococcus laurentii 11 biomass and lipids in cheese whey medium supplemented with sugarcane molasses. the optimization of ph, fermentation time, and molasses concentration according to a full factorial statistical experimental design was followed by a plackett-burman experimental design, which was used to determine whether the supplementation of the culture medium by yeast extract and inorganic salts coul ... | 2014 | 25242919 |
| sugarcane bagasse hydrolysis using yeast cellulolytic enzymes. | ethanol fuel production from lignocellulosic biomass is emerging as one of the most important technologies for sustainable development. to use this biomass, it is necessary to circumvent the physical and chemical barriers presented by the cohesive combination of the main biomass components, which hinders the hydrolysis of cellulose and hemicellulose into fermentable sugars. this study evaluated the hydrolytic capacity of enzymes produced by yeasts, isolated from the soils of the brazilian cerrad ... | 2013 | 23851270 |
| a biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse. | in this study, a co-production of two high value-added products, glucose and xylooligosaccharides (xos), was investigated by utilizing sugarcane bagasse (sb) within a multi-product bio-refinery framework optimized by box-behnken design-based response surface methodology. the developed process resulted in a maximum cellulose conversion of xylan-removed sb, 98.69±1.30%, and a maximum extracted sb xylan conversion into xos (xylobiose and xylotriose) of 57.36±0.79% that was the highest sb xylan conv ... | 2016 | 26773956 |
| possible involvement of maize rop1 in the defence responses of plants to viral infection. | the expression of host genes can be altered during the process of viral infection. to investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with sugarcane mosaic virus (scmv), a suppression subtractive hybridization cdna library was constructed. a total of 454 cdna clones were identified to be viral infection-induced up-regulated genes. the influence of rop1 on the infection of maize by scmv was investigated. the result ... | 2012 | 22332840 |
| use of pervaporation process for the recovery of aroma compounds produced by p. fermentans in sugarcane molasses. | natural fruity aroma was produced during submerged fermentation by pichia fermentans using sugarcane molasses as a cultivation broth. the aroma compounds were recovered from the fermentation by a pervaporation process using a polydimethylsiloxane membrane (pervap 4060-sulzer). isoamyl acetate, a characteristic compound associated with fruity aromas, was the major compound produced. the pervaporation module was fed at three different temperatures to test the best conditions to recover the natural ... | 2017 | 28303325 |
| gene silencing in root lesion nematodes (pratylenchus spp.) significantly reduces reproduction in a plant host. | root lesion nematodes (rlns, pratylenchus species) are a group of economically important migratory endoparasitic plant pathogens that attack host roots of major crops such as wheat and sugarcane, and can reduce crop yields by 7-15%. pratylenchus thornei and pratylenchus zeae were treated with double stranded rna (dsrna) to study gene silencing, (rna interference, rnai), as a potential strategy for their control. mixed stages of nematodes of both species ingested dsrna when incubated in a basic s ... | 2013 | 23201220 |
| valorization of agro-industrial wastes towards the production of rhamnolipids. | in this work, oil mill wastewater (omw), a residue generated during olive oil extraction, was evaluated as an inducer of rhamnolipid production. using a medium containing as sole ingredients corn steep liquor (10%, v/v), sugarcane molasses (10%, w/v) and omw (25%, v/v), pseudomonas aeruginosa #112 produced 4.5 and 5.1g of rhamnolipid per liter in flasks and reactor, respectively, with critical micelle concentrations as low as 13mg/l. furthermore, in the medium supplemented with omw, a higher pro ... | 2016 | 27092993 |
| antifungal properties of rhamnolipid produced by pseudomonas aeruginosa ds9 against colletotrichum falcatum. | the rhamnolipid biosurfactant (rl-ds9) extracted from the bacterial strain pseudomonas aeruginosa ds9 was evaluated for its antifungal activity against colletotrichum falcatum that causes red rot in sugarcane. the surface tension (st) reduction, biosurfactant production, and antifungal activity of biosurfactant against c. falcatum were investigated by using the medium with different carbon sources and it was found to be maximum in glucose. moreover, highest reduction of st and production of bios ... | 2015 | 26173581 |
| differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria. | the herbicides ametryn and clomazone are widely used in sugarcane cultivation, and following microbial degradation are considered as soil and water contaminants. the exposure of microorganisms to pesticides can result in oxidative damage due to an increase in the production of reactive oxygen species (ros). this study investigated the response of the antioxidant systems of two bacterial strains tolerant to the herbicides ametryn and clomazone. bacteria were isolated from soil with a long history ... | 2014 | 25380132 |
| high poly(β-hydroxybutyrate) production by pseudomonas fluorescens a2a5 from inexpensive substrates. | a strain of poly(β-hydroxybutyrate) (phb)-accumulating bacterium was isolated from the soil in alaska of usa, identified as pseudomonas fluorescens, and designated as strain a2a5. the organism grew at temperatures below 30°c, and accumulated a large amount of granules in its cells when it was cultured in the sugarcane liquor medium. the purified sample from cells was determined as phb by gas chromatographic and nuclear magnetic resonance analysis of polyesters. the cell density of the culture in ... | 2008 | 22578867 |
| genotypic and phenotypic diversity of pgpr fluorescent pseudomonads isolated from the rhizosphere of sugarcane (saccharum officinarum l.). | the genetic diversity of plant growth-promoting rhizobacterial (pgpr) fluorescent pseudomonads associated with the sugarcane (saccharum officinarum l.) rhizosphere was analyzed. selected isolates were screened for plant growthpromoting properties including production of indole acetic acid, phosphate solubilization, denitrification ability, and production of antifungal metabolites. furthermore, 16s rdna sequence analysis was performed to identify and differentiate these isolates. based on 16s rdn ... | 2012 | 22297215 |
| exposure to rabies in small indian mongooses (herpestes auropunctatus) from two regions in puerto rico. | the small indian mongoose (herpestes auropunctatus) was introduced to several caribbean islands to control rat (rattus spp.) damage to sugarcane plantations. mongooses failed at suppressing rat populations and are now considered pests throughout most of their introduced range. importantly, mongooses are rabies reservoirs on several caribbean islands. in puerto rico, mongooses have been implicated in up to 70% of reported animal rabies cases. there is no rabies vaccination program for wildlife in ... | 2015 | 26251987 |
| factors associated with the suppressiveness of sugarcane soils to plant-parasitic nematodes. | observations in three australian sugarcane fields suggested that the soil just under the trash blanket (the covering of crop residue that remains on the soil surface after crops are harvested) was suppressive to plant-parasitic nematodes. roots were concentrated in this upper layer of soil but plant-parasitic nematode populations were relatively low and roots showed few signs of nematode damage. root biomass was much lower 15 cm further down the soil profile, where root health was poor and popul ... | 2011 | 23431051 |
| characterization of a thermotolerant phytase produced by rhizopus microsporus var. microsporus biofilm on an inert support using sugarcane bagasse as carbon source. | the rhizopus microsporus var. microsporus biofilm was able to produce increased levels of an extracellular thermotolerant phytase using polyethylene and viscose as an inert support in both modified nbrip medium and modified khanna medium containing sugarcane bagasse as the carbon source. the enzyme production was strictly regulated by the phosphorus content with optimal production at 0.5 mm of sodium phytate and kh2po4. the extracellular phytase, rmphy1, was purified 4.18-fold with 4.78 % recove ... | 2016 | 26906117 |
| lipids of rhodotorula mucilaginosa iipl32 with biodiesel potential: oil yield, fatty acid profile, fuel properties. | this study analyzes the single cell oil (sco), fatty acid profile, and biodiesel fuel properties of the yeast rhodotorula mucilaginosa iipl32 grown on the pentose fraction of acid pre-treated sugarcane bagasse as a carbon source. the yeast biomass from nitrogen limiting culture conditions (15.3 g l(-1) ) was able to give the sco yield of 0.17 g g(-1) of xylose consumed. acid digestion, cryo-pulverization, direct in situ transesterification, and microwave assisted techniques were evaluated in com ... | 2017 | 28155998 |
| draft genome sequence of saccharomyces cerevisiae barra grande (bg-1), a brazilian industrial bioethanol-producing strain. | here, we present the draft genome sequence of saccharomyces cerevisiae bg-1, a brazilian industrial strain widely used for bioethanol production from sugarcane. the 11.7-mb genome sequence consists of 216 scaffolds and harbors 5,607 predicted protein-coding genes. | 2017 | 28360170 |
| microbial-enhanced lindane removal by sugarcane (saccharum officinarum) in doped soil-applications in phytoremediation and bioaugmentation. | the aim of this study was to examine the effect of lindane-degrading yeast on the growth and lindane uptake by saccharum sp., in doped garden soils. the rhizosphere of saccharum plant was amended with yeast candida vitjzn04 by root-inoculation. the bio-augment yeast was applied in two different forms viz., planktonic form and cells immobilized on sugarcane-bagasse, in the pot experiments. garden soils (lindane∼100 mg/kg) exposed to various treatments were monitored for a period of 30 days, for r ... | 2017 | 28259469 |
| the coptotermes gestroi aldo-keto reductase: a multipurpose enzyme for biorefinery applications. | in nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps. | 2017 | 28053664 |
| use of lignocellulose biomass for endoxylanase production by streptomyces termitum. | actinobacteria isolates from brazilian cerrado soil were evaluated for their ability to produce enzymes of the cellulolytic and xylanolytic complex using lignocellulose residual biomass. preliminary semiquantitative tests, made in petri plates containing carboxymethylcellulose and beechwood xylan, indicated 11 potential species producing enzymes, all belonging to the genus streptomyces. the species were subsequently grown in pure substrates in submerged fermentation and analyzed for the producti ... | 2017 | 28045607 |
| improvement production of hyaluronic acid by streptococcus zooepidemicus in sugarcane molasses. | microbial hyaluronic acid (ha) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. in this context, to obtain an economically competitive ha production by streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. the highest ha production by s. zooepidemicus atcc 39920 achieved was 2.825 g. l(-1) in a 4.5 l bioreactor with controlled ph (8.0) and medium containing molasses (85.3 ... | 2016 | 27900664 |
| use of sugarcane molasses by pycnoporus sanguineus for the production of laccase for dye decolorization. | pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. we evaluated laccase production in a recently isolated strain of p. sanguineus cultivated with sugarcane molasses as the only carbon source, and urea or yeast extract as the nitrogen source ... | 2016 | 27813609 |
| biosynthesis, purification and characterization of β-1,4-xylanase from a novel mangrove associated actinobacterium streptomyces olivaceus (msu3) and its applications. | the present study was undertaken to evaluate the xylanolytic properties of an actinobacterium streptomyces olivaceus (msu3) isolated from the sediment sample of mangrove environment. it showed highest xylanase activity on initial screening in breg's mineral salts medium supplemented with 0.5% xylan. further the organism expressed maximum xylanase production at optimized culture conditions of ph 7, temperature 30 °c with 2.5% of inoculum size at 72 h of incubation, the nutrient sources like sucro ... | 2017 | 27693622 |
| magnesium ions in yeast: setting free the metabolism from glucose catabolite repression. | in a recent work we showed that magnesium (mg(ii)) plays an important role in industrial ethanol production, overcoming the negative effect of the excess of minerals, particularly copper, present in sugarcane juice, with a consequent increase in ethanol yield. this cation has been reported to be involved in several steps of yeast metabolism, acting mainly as a co-factor of several enzymes of fermentation metabolism and protecting yeast cells from stressful conditions. however, despite many physi ... | 2016 | 27714092 |
| d-xylose fermentation, xylitol production and xylanase activities by seven new species of sugiyamaella. | sixteen yeast isolates identified as belonging to the genus sugiyamaella were studied in relation to d-xylose fermentation, xylitol production, and xylanase activities. the yeasts were recovered from rotting wood and sugarcane bagasse samples in different brazilian regions. sequence analyses of the internal transcribed spacer (its) region and the d1/d2 domains of large subunit rrna gene showed that these isolates belong to seven new species. the species are described here as sugiyamaella ayubii ... | 2017 | 27688209 |
| screening of yeasts for selection of potential strains and their utilization for in situ microbial detoxification (ismd) of sugarcane bagasse hemicellulosic hydrolysate. | many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. the conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. hence, it is imperative to explore biological methods to overcome the inhibitors by ... | 2016 | 27570309 |
| effect of ozonolysis pretreatment parameters on the sugar release, ozone consumption and ethanol production from sugarcane bagasse. | a l9(3)(4) orthogonal array (oa) experimental design was applied to study the four parameters considered most important in the ozonolysis pretreatment (moisture content, ozone concentration, ozone/oxygen flow and particle size) on ethanol production from sugarcane bagasse (scb). statistical analysis highlighted ozone concentration as the highest influence parameter on reaction time and sugars release after enzymatic hydrolysis. the increase on reaction time when decreasing the ozone/oxygen flow ... | 2016 | 27132222 |
| distribution of dekkera bruxellensis in a sugarcane-based fuel ethanol fermentation plant. | we investigated the presence of the yeast dekkera bruxellensis in samples collected at three points surrounding the industrial alcoholic fermentation plants of two distilleries where there are often cases of contamination caused by this yeast: this involved sugar cane wash water, feeding sugar cane juice and vinasse from the treatment pond. total yeast was isolated in wln medium with bromocresol green and cycloheximide and further selected on the basis of its ability to grow in synthetic medium ... | 2016 | 26928357 |
| enhancement of penicillium echinulatum glycoside hydrolase enzyme complex. | the enhancement of enzyme complex produced by penicillium echinulatum grown in several culture media components (bagasse sugarcane pretreated by various methods, soybean meal, wheat bran, sucrose, and yeast extract) was studied to increment fpase, xylanase, pectinase, and β-glucosidase enzyme activities. the present results indicated that culture media composed with 10 g/l of the various bagasse pretreatment methods did not have any substantial influence with respect to the fpase, xylanase, and ... | 2016 | 26922416 |
| development of flocculent saccharomyces cerevisiae strain gyk-10 for the selective fermentation of glucose/fructose in sugar mills. | advances in glucose/fructose-selective ethanol production have successfully enhanced raw sugar extraction from sugarcane juice by converting inhibitory substances (i.e., glucose/fructose) into ethanol, which is removed by subsequent operations in cane sugar mills. however, the commercial implementation of this breakthrough process in existing cane sugar mills requires a yeast strain that (i) can be used in food production processes, (ii) exhibits stable saccharometabolic selectivity, and (iii) c ... | 2016 | 26811219 |
| sucrose and saccharomyces cerevisiae: a relationship most sweet. | sucrose is an abundant, readily available and inexpensive substrate for industrial biotechnology processes and its use is demonstrated with much success in the production of fuel ethanol in brazil. saccharomyces cerevisiae, which naturally evolved to efficiently consume sugars such as sucrose, is one of the most important cell factories due to its robustness, stress tolerance, genetic accessibility, simple nutrient requirements and long history as an industrial workhorse. this minireview is focu ... | 2016 | 26658003 |
| biochemical conversion of sugarcane straw hemicellulosic hydrolyzate supplemented with co-substrates for xylitol production. | biotechnological production of xylitol is an attractive route to add value to a sugarcane biorefinery, through utilization of the hemicellulosic fraction of sugarcane straw, whose availability is increasing in brazil. herein, supplementation of the sugarcane straw hemicellulosic hydrolyzate (xylose 57gl(-1)) with maltose, sucrose, cellobiose or glycerol was proposed, and their effect as co-substrates on xylitol production by candida guilliermondii fti 20037 was studied. sucrose (10gl(-1)) and gl ... | 2016 | 26615771 |
| hemicellulosic ethanol production by immobilized wild brazilian yeast scheffersomyces shehatae ufmg-hm 52.2: effects of cell concentration and stirring rate. | the use of sugarcane bagasse hemicellulosic hydrolysates presents an interesting alternative to second generation (2g) ethanol production. techniques to enhance the fermentation process, e.g., the use of immobilized cells, is one of the key factors for efficient production. here, the effect of two important parameters (cell concentration in immobilized system and stirring rate) on the 2g ethanol production using the wild brazilian yeast s. shehatae ufmg-hm 52.2 immobilized in calcium alginate ma ... | 2016 | 26507335 |
| mixed substrate fermentation for enhanced phytase production by thermophilic mould sporotrichum thermophile and its application in beneficiation of poultry feed. | the optimum values of the critical variables determined by the central composite design of response surface methodology (rsm) for maximum phytase production (1881.26 u g(-1) dry mouldy residue (dmr)) by sporotrichum thermophile are 2.5 % tween 80, 1.0 % yeast extract and 48 h of incubation period. phytase production in the mixed substrate (sugarcane bagasse and wheat bran) fermentation enhanced 11.6-fold over the initial production as a consequence of optimization. phytase titres are sustainable ... | 2016 | 26433602 |
| mechanistic study on ultrasound assisted pretreatment of sugarcane bagasse using metal salt with hydrogen peroxide for bioethanol production. | this study presents the ultrasound assisted pretreatment of sugarcane bagasse (scb) using metal salt with hydrogen peroxide for bioethanol production. among the different metal salts used, maximum holocellulose recovery and delignification were achieved with ultrasound assisted titanium dioxide (tio2) pretreatment (uatp) system. at optimum conditions (1% h2o2, 4 g scb dosage, 60 min sonication time, 2:100 m ratio of metal salt and h2o2, 75°c, 50% ultrasound amplitude and 70% ultrasound duty cycl ... | 2016 | 26384901 |
| development of a novel sequential pretreatment strategy for the production of bioethanol from sugarcane trash. | a novel sequential pretreatment strategy using biodiesel industry generated waste glycerol assisted transition metal and alkali pretreatment of sugarcane trash were developed for the production of bioethanol. various process parameters affecting pretreatment as well as hydrolysis were optimized by adopting a taguchi design. this novel method was found to be superior when compared to conventional pretreatment strategies like acid and alkali in removing hemicelluloses and lignin and the hydrolyzat ... | 2016 | 26318846 |
| in vitro ability of beer fermentation residue and yeast-based products to bind aflatoxin b1. | this study aimed to verify the in vitro ability of beer fermentation residue (bfr) containing saccharomyces cerevisiae cells and five commercial products that differed in the viability and integrity of s. cerevisiae cells to remove aflatoxin b1 (afb1) from a citrate-phosphate buffer solution (cpbs). bfr was collected at a microbrewery and prepared by drying and milling. the commercial yeast-based products were as follows: inactive intact yeast cells from beer alcoholic fermentation, inactive int ... | 2015 | 26273277 |
| statistical optimization of laccase production and delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation. | laccases are oxidative enzymes related to the degradation of phenolic compounds, including lignin units, with concomitant reduction of oxygen to water. delignification is a necessary pretreatment step in the process of converting plant biomass into fermentable sugars. the objective of this work was to optimize the production of laccases and to evaluate the delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation. among eight variables (ph, water activity, temperatu ... | 2015 | 26180784 |
| 2g ethanol from the whole sugarcane lignocellulosic biomass. | in the sugarcane industry, large amounts of lignocellulosic residues are generated, which includes bagasse, straw, and tops. the use of the whole sugarcane lignocellulosic biomass for the production of second-generation (2g) ethanol can be a potential alternative to contribute to the economic viability of this process. here, we conducted a systematic comparative study of the use of the lignocellulosic residues from the whole sugarcane lignocellulosic biomass (bagasse, straw, and tops) from comme ... | 2015 | 25774217 |
| mannosylerythritol lipids: production and applications. | mannosylerythritol lipids (mels) are a glycolipid class of biosurfactants produced by a variety yeast and fungal strains that exhibit excellent interfacial and biochemical properties. mel-producing fungi were identified using an efficient screening method for the glycolipid production and taxonomical classification on the basis of ribosomal rna sequences. mel production is limited primarily to the genus pseudozyma, with significant variability among the mel structures produced by each species. o ... | 2015 | 25748373 |
| characterization of a protein-protein interaction network of the cbl-interacting protein kinase 8 from sugarcane. | plants are sessile organisms and have evolved to tolerate a constantly changing environment. after the onset of different stress conditions, calcineurin b-like (cbl) proteins can sense calcium signals and activate cbl-interacting protein kinase (cipk) proteins, which can phosphorylate downstream proteins to reestablish plant homeostasis. previous studies in the bioenergy crop sugarcane showed that the sccipk8 gene is induced by drought stress and is also related to sucrose content. here, we have ... | 2015 | 25729982 |