| evidence for widespread afferents to barrington's nucleus, a brainstem region rich in corticotropin-releasing hormone neurons. | supraspinal afferents to the pontine micturition center, barrington's nucleus, were investigated in the rat by visualization of the retrograde tracer, cholera-toxin subunit b, in neurons following iontophoretic injection into barrington's nucleus. tissue sections from five rats with injections primarily localized in barrington's nucleus revealed numerous retrogradely labeled neurons throughout all rostrocaudal levels of the periaqueductal gray (particularly its ventrolateral division), in the la ... | 1994 | 7816195 |
| neurochemical identification of a13 dopaminergic neuronal projections from the medial zona incerta to the horizontal limb of the diagonal band of broca and the central nucleus of the amygdala. | studies utilizing fluorescent histochemical techniques first revealed that a13 dopaminergic (da) perikarya located in medial zona incerta (mzi) project to various regions within the hypothalamus; accordingly, these da neurons were designated the 'incertohypothalamic' da neuronal system. more recently, it has been shown that the anterograde neuronal tract tracer phaseolus vulgaris leucoagglutinin, after injection into mzi, is identified in nerve terminals outside of the hypothalamus; for example, ... | 1994 | 7820662 |
| neocortex provides direct synaptic input to interstitial neurons of the intermediate zone of kittens and white matter of cats: a light and electron microscopic study. | the existence of direct synaptic input from the neocortex to intermediate zone and white matter interstitial neurons was examined in both neonate and adult cats. this projection was studied by injecting the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) into the neocortex and examining whether cortical efferent axons formed synapses in the intermediate zone or white matter. anterogradely labeled boutons establishing synapses in the intermediate zone and white matter were found at ... | 1994 | 7822492 |
| fast purification of phaseolus vulgaris isolectins. | phytohemagglutinin from red kidney bean has been purified by affinity chromatography on a human alpha 1-acid glycoprotein sepharose 4b column. further purification of the hemagglutinin's five isolectins was achieved on a mono s column with an 86% protein recovery. each sequentially eluted isolectin from the ion exchange column displayed either hemagglutinating or mitogenic activity. the main activity of each fraction was the result of the combination of varying proportions of the l and e subunit ... | 1994 | 7831200 |
| connectivity of the lobus parolfactorius of the domestic chicken (gallus domesticus): an anterograde and retrograde pathway tracing study. | in 1-week-old domestic chicks, the connectivity of the lobus parolfactorius (lpo), part of the avian basal ganglia, was investigated using phaseolus vulgaris leucoagglutinin and horseradish peroxidase for anterograde and retrograde pathway tracing, respectively. tyrosine hydroxylase immunocytochemistry was applied in combination with phaseolus lectin to assess the overlap between lpo efferents and diencephalic and mesencephalic catecholamine centres. anterograde projections from lpo were detecte ... | 1994 | 7844254 |
| organization of vestibular nucleus projections to the caudal dorsal cap of kooy in rabbits. | this study used retrograde and anterograde tracing methods to characterize the origin and terminal distribution of vestibular nuclear projections to the caudal dorsal cap of the inferior olive in albino rabbits. comparisons of the retrograde labeling patterns from cholera toxin b fragment-horseradish peroxidase and fluoro-gold injection sites centred in either the caudal dorsal cap or the rostral dorsal cap plus ventrolateral outgrowth revealed that they receive projections from different vestib ... | 1994 | 7845595 |
| lectin-histochemical analysis of glycans in ovine and bovine near-term placental binucleate cells. | chorionic binucleate cells (bnc) occur in several ruminants including cow, deer, goat and sheep. they migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. we have compared the bnc of near-term, resin-embedded, ovine and bovine placentae using 15 biotinylated lectins and an avidinperoxidase revealing system. there was pronounced conservation of saccharides between the two species. several sub-types of n-gl ... | 1994 | 7850870 |
| effect of lectins on mouse peritoneal macrophage phagocytic activity. | we studied the in vitro ability of lectin-treated murine peritoneal macrophages to attach and phagocytize particulate antigens. glucose and mannose specific lectins such as con-a and lentil lectin, as well as complex lactosamine residues specific lectins, such as phaseolus vulgaris var. cacahuate and phaseolus coccineus var. alubia, increased the macrophage phagocytic activity towards heterologous erythrocytes, whereas peanut agglutinin, a galactose-specific lectin, diminished the macrophage pha ... | 1994 | 7851961 |
| c-terminal post-translational proteolysis of plant lectins and their recombinant forms expressed in escherichia coli. characterization of "ragged ends" by mass spectrometry. | electrospray mass spectrometry was used to accurately measure the molecular masses of single chain lectins from legume seeds and also of three recombinant lectins, expressed in escherichia coli. the five single chain lectins, erythrina corallodendron lectin, soybean and peanut agglutinins, dolichos biflorus lectin, and phaseolus vulgaris hemagglutinin e, all showed evidence of c-terminal proteolytic processing, in some cases to "ragged" ends, when their masses were compared to those expected fro ... | 1995 | 7852319 |
| changes in dietary fiber content and its composition as affected by processing of black beans (phaseolus vulgaris, tamazulapa variety). | this paper presents the effect that the traditional cooking process of black beans (phaseolus vulgaris, tamazulapa variety) has on the quantity and composition of soluble (sdf) and insoluble (idf) dietary fiber of beans, as well as on its protein digestibility and protein quality. there was an increase of idf from 18.1% in cooked beans to 22.4% in fried beans, and a decrease in sdf from 8.4% to 6.6%, respectively. starch content decreased from 34.5% to 31.3%. no change was found in lignin. the x ... | 1994 | 7855081 |
| consumption of diets containing raw soya beans (glycine max), kidney beans (phaseolus vulgaris), cowpeas (vigna unguiculata) or lupin seeds (lupinus angustifolius) by rats for up to 700 days: effects on body composition and organ weights. | feeding trials have been done with rats to assess the effects of long-term (700 d) consumption of diets based on raw cowpeas (vigna unguiculata; moderate bowman-birk inhibitor content, low lectin content), lupin seeds (lupinus angustifolius; low lectin and protease inhibitor content) or soya beans (glycine max; high kunitz inhibitor content, moderate bowman-birk inhibitor content, moderate lectin content) or diets containing low levels of raw kidney bean (phaseolus vulgaris; high lectin content, ... | 1995 | 7857911 |
| the expression of a chimeric phaseolus vulgaris nodulin 30-gus gene is restricted to the rhizobially infected cells in transgenic lotus corniculatus nodules. | in phaseolus vulgaris there is a nodulin family, npv30, of ca. 30 kda, as detected in an in vitro translation assay [2]. we isolated a gene (npv30-1) for one of the members of this family. the nucleotide sequence of the promoter of npv30-1 contains nodule-specific motifs common to other late nodulin genes. the promoter was fused to the gus reporter gene; this chimeric fusion was introduced into lotus corniculatus via agrobacterium rhizogenes transformation. gus activity was only detected in the ... | 1994 | 7858234 |
| quantitative electron microscopic analysis of synaptic input from cortical areas 17 and 18 to the dorsal lateral geniculate nucleus in cats. | cortical feedback is the largest extraretinal projection to the lateral geniculate nucleus. this input is thought to modulate the transfer of visual information in a state-dependent manner. the quantitative distribution and synaptology of axon terminals arising from different cortical areas is still an unsolved question. to address this problem, the synaptic termination pattern of corticogeniculate axons from cortical areas 17 and 18 entering the lateral geniculate nucleus of the cat was examine ... | 1994 | 7860782 |
| structure and expression of a nitrite reductase gene from bean (phaseolus vulgaris) and promoter analysis in transgenic tobacco. | a structural gene encoding nitrite reductase (nir) in bean (phaseolus vulgaris) has been cloned and sequenced. the nir gene is present as a single copy encoding a protein of 582 amino acids. the bean nir protein is synthesized as a precursor with an amino-terminal transit peptide (tp) consisting of 18 amino acid residues. the bean nir transit peptide shows similarity to the tps of other known plant nirs. the nir gene is expressed in trifoliate leaves and in roots of 20-day old bean plants where ... | 1995 | 7865786 |
| an indirect projection from the nucleus of the solitary tract to the central nucleus of the amygdala via the parabrachial nucleus in the rat: a light and electron microscopic study. | the morphological basis of how visceral information from the nucleus of the solitary tract (nts) is relayed from the parabrachial nucleus (pbn) to the central nucleus of the amygdala (ce) was studied at the light and electron microscopic levels using the anterograde tracer, phaseolus vulgaris leucoagglutinin (pha-l), kainic acid degeneration, and retrograde tracing with horseradish peroxidase (hrp). after injection of pha-l into the caudal nts, anterogradely labeled fibers and terminals were pre ... | 1994 | 7874500 |
| reactivity of gliadin and lectins with celiac intestinal mucosa. | the binding patterns of gliadin and selected lectins to jejunal biopsy specimens obtained from children with total villous atrophy during active celiac disease (cd; 19 patients) and in remission (16 patients) were examined by light microscopy. three categories of carbohydrate-specific lectins were chosen for the study: those recognizing mannose/glucose residues, those recognizing n-acetyl-glucosamine/glucose (glcnac/glc) residues, and those recognizing n-acetylgalactosamine/galactose (galnac/gal ... | 1994 | 7877884 |
| [the human spleen in idiopathic thrombocytopenic purpura]. | idiopathic (autoimmune) thrombocytopenic purpura (itp, aitp) represents a relatively frequent impairment. it involves a syndrome of various diseases with a shortened thrombocytes survival caused by anti-platelet antibodies. the majority of cases are of secondary character. spleenectomy often evokes a complete remission of thrombocytopenia. the study describes morphologic findings in spleens of 30 patients with the clinical diagnosis of itp/aitp. the findings were gained by light microscopy from ... | 1994 | 7882065 |
| wild type rhizobium etli, a bean symbiont, produces acetyl-fucosylated, n-methylated, and carbamoylated nodulation factors. | phaseolus vulgaris (common bean) can be nodulated by different rhizobium species. a new species has been recently proposed: rhizobium etli. following transcriptional activation of the bacterial nodulation genes using naringenin or bean seed exudate, we have isolated, purified, and characterized r. etli extracellular nodulation factors. they are chitopentameric compounds that are n-methyl-n-vaccenoylated at their non-reducing end. at position 6 of the reducing n-acetyl-d-glucosamine, they are 4-o ... | 1995 | 7890737 |
| associational and commissural afferents of parvalbumin-immunoreactive neurons in the rat hippocampus: a combined immunocytochemical and pha-l study. | nonpyramidal neurons containing the calcium-binding protein parvalbumin (pv) are one of the inhibitory elements of the hippocampal network. previous studies have indicated that they are involved in septohippocampal disinhibitory circuits. this study analyzes the commissural and ipsilateral associational afferents of parvalbumin neurons. injections of the anterograde tracer phaseolus vulgaris-leucoagglutinin (pha-l) into the hilus of the fascia dentata labeled numerous axons in the molecular laye ... | 1994 | 7890833 |
| field bean protease inhibitor preparations, unlike methotrexate, can completely suppress yoshida sarcoma tumor in rats. | protease inhibitor preparations (pip) with antitryptic and antichymotryptic activities, isolated from field bean legume as well as doxorubicin and cyclophosphamide could effectively suppress the growth of yoshida sarcoma ascites tumor cells transplanted in adult rats and prevent their death. as against this, methotrexate and heat-inactivated pip were ineffective in such rats at varied doses of treatment tried. the percent survival of animals appeared to be related to the purity, treatment mode a ... | 1994 | 7894388 |
| a1 catecholamine cell group: fine structure and synaptic input from the nucleus of the solitary tract. | preembedding immunoperoxidase staining methods were used to characterize tyrosine hydroxylase-immunoreactive (th-ir) elements in the caudal ventrolateral medulla, and to determine the extent to which neurons of the a1 cell group are directly innervated by projections of the nucleus of the solitary tract (nts). th-ir neurons in the a1 region were medium-sized and multipolar. they possessed rounded nuclei with infrequent invaginations, well-developed golgi apparati, high cytoplasmic densities of m ... | 1995 | 7896940 |
| descending efferent connections of the sub-pallidal areas in the cat: projections to the subthalamic nucleus, the hypothalamus, and the midbrain. | the efferent connections of the sub-pallidal regions to the mediodorsal thalamic nucleus, the subthalamic nucleus, the lateral hypothalamic area, and the midbrain were investigated in the cat, using phaseolus vulgaris--leucoagglutinin (pha-l) as an anterograde label. the results indicate that the sub-pallidal regions of the cat project to the (dorso)medial tip of the subthalamic nucleus and the adjoining lateral hypothalamic area as well as to the ventral tegmental area and the greater extent of ... | 1993 | 7903124 |
| tubular atrophy in the end-stage kidney: a lectin and immunohistochemical study. | atrophic tubules in end-stage renal disease (esrd) may have various morphologic appearances: some show microscopic features of "classic" atrophic tubules (thick, wrinkled tubular basement membrane and simplified epithelium), others show "thyroidization" (round tubules with simplified epithelium and casts), and many have the appearance of "endocrine" tubules (small tubules with narrow lumina, clear cells, and relatively thin basement membranes). other tubules in esrd may be enlarged and dilated w ... | 1994 | 7906246 |
| intra-amygdaloid projections of the lateral nucleus in the cat: pha-l anterograde labeling combined with postembedding gaba and glutamate immunocytochemistry. | research on the implication of the amygdala in classical fear conditioning suggests that the central amygdaloid nucleus is the output station of the amygdala for conditioned fear responses, while the lateral nucleus acts as the input nucleus, at least for auditory conditioned stimuli. however, the nature and locus of the plastic changes taking place between these two nuclei are unknown partly because the neurotransmitter(s) used by intra-amygdaloid projections of the lateral nucleus has not been ... | 1994 | 7911130 |
| synaptic relationships between dopaminergic afferents and cortical or thalamic input in the sensorimotor territory of the striatum in monkey. | the cerebral cortex and the intralaminar thalamic nuclei are the major sources of excitatory glutamatergic afferents to the striatum, whereas the midbrain catecholaminergic neurones provide a dense intrastriatal plexus of dopamine-containing terminals. evidence from various sources suggests that there is a functional interaction between the glutamate- and dopamine-containing terminals in the striatum. the aim of the present study was to determine the synaptic relationships between cortical or th ... | 1994 | 7914894 |
| multiple striatal representation in primate substantia nigra. | the pattern of arborization of the striatonigral fibers in the squirrel monkey (saimiri sciureus) was studied with phaseolus vulgaris-leucoagglutinin (pha-l) and biocytin as anterograde tracers. single, small injections of pha-l or biocytin in either the caudate nucleus or the putamen give rise to at least four distinct, nonoverlapping but interconnected fiber plexuses that are distributed throughout the rostrocaudal extent of the substantia nigra pars reticulata (snr) according to a strikingly ... | 1994 | 7915728 |
| characterization of a bean (phaseolus vulgaris l.) malic-enzyme gene. | we have isolated a genomic clone encoding a plant nadp(+)-dependent malic enzyme (nadp-me). this clone, isolated from bean (phaseolus vulgaris l.), covers the entire gene (exons, introns) and 5'-flanking regions. dna sequencing defines 20 exons spanning approximately 4.5 kb, which range over 48-235 bp in size. all 19 introns are fairly small (79-391). the first intron resides in the 5'-untranslated leader sequence. introns 10, 11 and 16 are located at positions identical to a rat malic-enzyme ge ... | 1994 | 7925425 |
| a rhizobium tropici dna region carrying the amino-terminal half of a nodd gene and a nod-box-like sequence confers host-range extension. | rhizobium tropici ciat899 is a broad-host-range strain that, in addition to phaseolus, nodulates other plant legumes such as leucaena and macroptilium. the narrow-host-range of rhizobium leguminosarum biovars phaseoli (strain ce3) and trifolii (strain rs1051) can be extended to leucaena esculenta and phaseolus vulgaris plants, respectively, by the introduction of a dna fragment 521 bp long, which carries 128 amino acids of the amino-terminal region of a nodd gene from r. tropici, as well as a pu ... | 1993 | 7934929 |
| anatomical characterization of a novel reticulospinal vasodepressor area in the rat medulla oblongata. | microinjection of l-glutamate into a subregion of the gigantocellular nucleus of the rat medulla oblongata significantly lowers arterial pressure. this vasodepressor area, the gigantocellular depressor area, is topographically distinct from other vasoactive areas of the medulla. we sought to determine the efferent projections of the gigantocellular depressor area and compare these to the efferent projections of sympathoexcitatory neurons within the rostral ventrolateral medulla. the anterograde ... | 1994 | 7936200 |
| analysis of the efferent projections of the lateral geniculate nucleus with special reference to the innervation of the subcommissural organ and related areas. | in order to define central neurons projecting to the subcommissural organ (sco) and to related areas in the postero-medial diencephalon, phaseolus vulgaris-leucoagglutinin (pha-l) was injected into the lateral geniculate nucleus of the rat. pha-l-labelled neurons send axonal processes medially through the posterior thalamic nuclei and the posterior commissure to the other hemisphere. branches of fibres originating from this projection form a plexus of nerve terminals in the underlying precommiss ... | 1994 | 7954683 |
| isolation and characterization of a diagnostic polysaccharide antigen from larval echinococcus multilocularis. | a diagnostic antigen, c-antigen, was evaluated in serodiagnosis of alveolar hydatid disease by western blotting. the c-antigen migrated diffusely to the region of 30-35 kda in 8% polyacrylamide-sds gel electrophoresis. we isolated this antigen from a crude metacestode extract by a simple method of phenol/chloroform extraction. the isolated antigen was resistant to boiling, proteolysis and acid hydrolysis, but destroyed with sodium periodate. such lectins as peanut agglutinin (pna), ricinus commu ... | 1994 | 7967161 |
| distribution and localization of bean common mosaic virus and bean black root virus in stems of doubly infected bean plants. | plants of bean (phaseolus vulgaris) inoculated first on one primary leaf with strain ny15 of bean common mosaic virus, as inducer, and after three days, on the opposite leaf, with the strain nl3 of bean black root virus, as challenger, did not show systemic necrosis characteristic of the latter strain. this interference phenomenon was studied by determining the amount, distribution and localization of both strains in the part of stem between primary leaves and first trifoliolate leaf in both cha ... | 1994 | 7980014 |
| the nodd proteins of rhizobium sp. strain br816 differ in their interactions with coinducers and in their activities for nodulation of different host plants. | the early steps of symbiotic nodule formation by rhizobium spp. on plants require coordinate expression of several nod gene operons, which is accomplished by the activating protein nodd. rhizobium sp. strain br816, isolated from leucaena leucocephala, contains four nodd genes which differ in their interaction with flavonoids. two of the four nodd proteins, namely, nodd1 and nodd2, obey the lysr rule regarding the need of a coinducer. nodd3 shows hardly any inducing activity, and nodd4 contains a ... | 1994 | 7986038 |
| purification and characterization of the ecto-mg-atpase of chicken gizzard smooth muscle. | the ecto-mg-atpase isolated from chicken gizzard smooth muscle was solubilized, purified and characterized. the purification did not require the use of expensive or specialized apparatus. the chromatographic and electrophoretic characteristics of the ecto-mg-atpase from chicken are similar to those reported earlier for the ecto-mg-atpase isolated from rabbit skeletal muscle transverse tubule membranes [1992, j. biol. chem. 267, 11777-11782]. one obvious difference found was that the solubilized ... | 1994 | 7989647 |
| seed-specific gene activation mediated by the cre/lox site-specific recombination system. | the cre/lox site-specific recombination system was used to activate a transgene in a tissue-specific manner. cre-mediated activation of a beta-glucuronidase marker gene, by removal of a lox-bounded blocking fragment, allowed the visualization of the activation process. by using seed-specific promoters, the timing and efficiency of gene activation could be followed within the developing tobacco (nicotiana tabacum) embryo. to serve as a basis for analyzing gene expression after-cre-mediated activa ... | 1994 | 7991679 |
| galactose-containing glycoconjugates of the ciliary body and lens in capsular glaucoma: a lectin histochemical study. | this study was carried out in order to obtain information on galactose-containing glycoconjugates in the lens and ciliary body of human eyes with capsular glaucoma and thus on the etiopathogenesis of exfoliation syndrome. | 1994 | 8001826 |
| insoluble dietary fiber of grain food legumes and protein digestibility. | the present work aimed to verify the digestibility of cooked whole food grain legumes. samples of beans (phaseolus vulgaris and vigna sinensis), chickpeas (cicer arietinum) and lentils (lens culinaris) were used in the experiment. the interrelationship between the insoluble dietary fiber presented in the food grain legumes and the low protein digestibility was studied. the insoluble dietary fiber and the proteic nitrogen presented in the neutral detergent fiber (ndf) were determined. "in vivo" d ... | 1993 | 8002707 |
| genetic characterization of rhizobium sp. strain tal1145 that nodulates tree legumes. | rhizobium sp. strain tal1145 nodulates leucaena leucocephala and phaseolus vulgaris, in addition to a wide range of tropical tree legumes. six overlapping clones that complemented nodulation defects in leucaena and bean rhizobia were isolated and a 40-kb map of the symbiosis region was constructed. the common nod and nifa genes were situated approximately 17 kb apart, with the nodij genes in between. these clones enabled a derivative of tal1145 carrying a partially deleted psym to form ineffecti ... | 1994 | 8012908 |
| efferents of the opiocortin-containing region of the commissural nucleus tractus solitarius. | efferent projections of the commissural nucleus tractus solitarius (cnts0 in the region containing opiocortin-immunoreactive (-ir) neurons were identified using phaseolus vulgaris leucoagglutinin (pha-l). efferents were identified in the bed nucleus of the stria terminalis, preoptic area, amygdala, hypothalamus, periaqueductal gray, parabrachial nucleus, locus coeruleus, medullary catecholaminergic groups, and nts. the pha-l-ir varicosities in lateral parabrachial nucleus were identified in clos ... | 1994 | 8015975 |
| t-cell deficiency in suffolk lambs. | in a flock of suffolk sheep respiratory diseases were regularly observed, while a flock of booroola sheep kept under similar feeding and management conditions remained healthy. experiments were conducted to compare the immunological and haematological parameters of suffolk and booroola sheep of different age groups. the percentage of t and b cells in the lymphocyte population and the capacity for blastogenesis induced by nonspecific mitogens were analyzed. suffolk sucking lambs had significantly ... | 1993 | 8017245 |
| polygalacturonase-inhibiting protein accumulates in phaseolus vulgaris l. in response to wounding, elicitors and fungal infection. | polygalacturonase-inhibiting protein (pgip) is a cell wall-associated protein that specifically binds to and inhibits the activity of fungal endopolygalacturonases. the phaseolus vulgaris gene encoding pgip has been cloned and characterized. using a fragment of the cloned pgip gene as a probe in northern blot experiments, it is demonstrated that the pgip mrna accumulates in suspension-cultured bean cells following addition of elicitor-active oligogalacturonides or fungal glucan to the medium. ra ... | 1994 | 8019588 |
| convergent synaptic input from the neostriatum and the subthalamus onto identified nigrothalamic neurons in the rat. | the two major afferents of the substantia nigra pars reticulata are the subthalamic nucleus and the striatum. stimulation of these afferents has opposing physiological effects on the output neurons of the substantia nigra pars reticulata. in order to better understand the role of these afferents in the flow of information through the basal ganglia and to better understand the ways in which they might interact, experiments have been performed to test the possibility that single-output neurons of ... | 1994 | 8019671 |
| direct projections from the entopeduncular nucleus to the lower brainstem in the rat. | the present study reports the existence of projection fibers from the entopeduncular nucleus to the superior colliculus and lateral parts of the pontobulbar tegmental regions (so-called lateral tegmental field) in the rat, suggesting that the entopeduncular nucleus may control eye-head and orofacial movements via these projection fibers. the anterograde axonal tracing with phaseolus vulgaris-leucoagglutinin has revealed that the entopedunculotectal fibers terminate, bilaterally, with an ipsilate ... | 1994 | 8021342 |
| synaptic innervation of neurones in the internal pallidal segment by the subthalamic nucleus and the external pallidum in monkeys. | in order to better understand the way by which the subthalamic nucleus interacts with the globus pallidus to control the output of the basal ganglia, we carried out a series of experiments to investigate the pattern of synaptic innervation of the pallidal neurones by the subthalamic terminals in the squirrel monkey. to address this problem we used the anterograde transport of biocytin. following injections of biocytin in the subthalamic nucleus, rich plexuses of labelled fibres and varicosities ... | 1994 | 8027445 |
| effect of baked beans (phaseolus vulgaris) on steroid metabolism and non-starch polysaccharide output of hypercholesterolaemic pigs with or without an ileo-rectal anastomosis. | the plasma-cholesterol-lowering effects of some dietary legumes are now well established from animal and human studies, but the mechanism is not completely understood. the present study investigated the effect of baked beans (phaseolus vulgaris) on steroid metabolism of hypercholesterolaemic pigs. three groups of four pigs were studied: baseline (bl), normal pigs (np) and those previously prepared with an ileo-rectal anastomosis to nullify the function of the large intestine (ir). all three grou ... | 1994 | 8031736 |
| topographical relations between ipsilateral cortical afferents and callosal neurons in the second somatic sensory area of cats. | experiments were carried out on the second somatic sensory area (sii) of cats to study 1) the laminar distribution of axon terminals from the ipsilateral first somatic sensory cortex (si); and 2) the topographical relations between their terminal field and the callosal neurons projecting to the contralateral homotopic cortex. to label simultaneously in sii both ipsilateral cortical afferents and callosal cells, cats were given iontophoretic injections of phaseolus vulgaris-leucoagglutinin (pha-l ... | 1994 | 8034789 |
| vestibular nucleus projections to nucleus tractus solitarius and the dorsal motor nucleus of the vagus nerve: potential substrates for vestibulo-autonomic interactions. | autonomic effects of vestibular stimulation are important components of phenomena as diverse as acute vestibular dysfunction and motion sickness. however, the organization of neural circuits mediating these responses is poorly understood. this study presents evidence for direct vestibular nucleus projections to brain stem regions that mediate autonomic function. one group of albino rabbits received injections of phaseolus vulgaris leucoagglutinin into the vestibular nuclei. the tracer was visual ... | 1994 | 8050507 |
| the degradation of lectins, phaseolin and trypsin inhibitors during germination of white kidney beans, phaseolus vulgaris l. | white kidney beans (phaseolus vulgaris), cv processor, contain a relatively high content of phaseolin (storage protein), lectins and a special group of glycoproteins as well as a considerable amount of protein-type trypsin inhibitors. protein digestion of raw 'processor' beans in monogastrics, for example pigs, is disturbed by poorly digested, phaseolin lectins, which can bind to carbohydrates in brush border membranes of the small intestinal epithelium, and trypsin inhibitors. the effect of the ... | 1994 | 8052578 |
| efferent connections of the hypothalamic "aggression area" in the rat. | the efferent connections of the hypothalamic area of the rat, where attack behaviour can be elicited by electrical stimulation, were studied using iontophoretic injections of phaseolus vulgaris-leucoagglutinin. specificity for the hypothalamic "attack area" was investigated by comparison with efferents of hypothalamic sites outside the attack area. the hypothalamic attack area consists of the intermediate hypothalamic area and the ventrolateral pole of the ventromedial hypothalamic nucleus. fibr ... | 1994 | 8058117 |
| effect of tannins on starch digestibility and tmen of triticale and semipurified starches from triticale and field beans. | 1. the effects of field bean tannin-containing hulls (t-hulls), non-tannin hulls (nt-hulls) and tannin extract on starch digestibilities and tmen of triticale and semipurified starches from triticale and field beans were evaluated with adult cockerels and 3-week-old-chicks. 2. for both cockerels and chicks, the digestibility and tmen of semipurified starches from triticale were higher than those from beans. starch digestibilities of triticales (range 99.1 to 97.7%), of semipurified starches from ... | 1994 | 8062111 |
| phaseolus vulgaris lectin labelled and gaba immunogold stained terminals in nucleus rotundus. an em study. | having injected phaseolus vulgaris lectin (pha-l) iontophoretically to subpretectal (sp) and postero-ventralis (pv) thalamic nuclei, labelled fibers and terminals appeared in nucleus rotundus (rt). the pha-l immunostained fibers are divided dichotomically and they develop long terminal sections with large varicosities. in em preparates pha-l labelled terminals were observed establishing axo-somatic, axo-dendritic symmetrical synapses. these terminals were stained by gaba immunogold procedure and ... | 1994 | 8064141 |
| pedunculopontine nucleus in the squirrel monkey: projections to the basal ganglia as revealed by anterograde tract-tracing methods. | the efferent projections of the pedunculopontine nucleus (ppn) to the basal ganglia have been studied in the squirrel monkey (saimiri sciureus) with [3h]leucine and phaseolus vulgaris-leucoagglutinin (pha-l) as anterograde tracers. following unilateral injections of [3h]leucine or pha-l in the central portion of the ppn, numerous autoradiographic linear profiles or pha-l-labeled fibers ascend to the forebrain, both ipsilaterally and contralaterally. these fibers form a compact bundle that course ... | 1994 | 8077458 |
| dietary effect of phytogenic phytase and an addition of microbial phytase to a diet based on field beans, wheat, peas and barley on the utilization of phosphorus, calcium, magnesium, zinc and protein in piglets. | the effect of the addition of microbial phytase to a diet based on field beans (30%), wheat (28%), peas (25%), and barley (14%) was studied in a 2-week experiment with 3 x 8 castrated male, individually housed, hybrid piglets (live weight range 12-16 kg). all diets contained about 4.7 g ca, 4.2 g p (77% present as phytate phosphorus), 1.0 g mg, 60 mg zn per kg diet, and 17% crude protein. group i was fed the basal diet with a native phytase-activity of about 260 u per kg diet. in group ii, 350 u ... | 1994 | 8079507 |
| spinothalamic projections in amphibians as revealed with anterograde tracing techniques. | direct spinothalamic pathways were demonstrated in anurans (rana ridibunda, xenopus laevis) and in the ribbed newt, pleurodeles waltl. with the powerful anterograde tracers phaseolus vulgaris leucoagglutinin and biotinylated dextran amine, rather extensive spinothalamic projections were found, including the ventromedial thalamic nucleus, the dorsal thalamus and several posterior diencephalic nuclei (anurans), and the neuropil lateral to the pars ventralis thalami as well as to the anteroventral ... | 1994 | 8084504 |
| distribution of the postsynaptic dorsal column projection in the cuneate nucleus of monkeys. | cells in the spinal cord that are postsynaptic to primary afferent fibers project to the dorsal column nuclei in the postsynaptic dorsal column pathway. the projection of cells in the cervical spinal cord of monkeys to the cuneate nucleus has been reported to avoid pars rotunda of that nucleus, the part that contains the somatotopic representation of the ipsilateral hand. we used the sensitive anterograde tracer phaseolus vulgaris leucoagglutinin to reexamine this projection. we made multiple io ... | 1994 | 8089278 |
| whitefly transmission and efficient ssdna accumulation of bean golden mosaic geminivirus require functional coat protein. | bean golden mosaic geminivirus (bgmv) has a bipartite genome composed of two circular ssdna components (dna-a and dna-b) and is transmitted by the whitefly, bemisia tabaci. dna-a encodes the viral replication proteins and the coat protein. to determine the role of bgmv coat protein systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the bgmv coat protein gene. all three coat protein mutants produced systemic infections when coinoc ... | 1994 | 8091659 |
| [cortical projections from the reuniens nucleus of the thalamus in the rat]. | direct projections from the reuniens nucleus to the cerebral cortex were examined using the anterograde tracing method of phaseolus vulgaris leucoagglutinin (pha-l) in the rat. the reuniens nucleus was found to project to widespread cortical regions. the labeled fibers from the reuniens nucleus terminated in layers i, iii and iv of the frontal cortex including the medial and ventrolateral orbital cortices, the cingulate cortex, and the infralimbic cortex. a few fibers from the nucleus reuniens a ... | 1994 | 8091944 |
| human-specific c-neu proto-oncogene protein overexpression in human malignant astrocytomas before and after xenografting. | overexpression of human-specific c-neu proto-oncogene transmembrane tyrosine kinase receptor protein (p185) is an index of cell transformation and of poor patient survival in several malignancies. the authors studied this protein in low- and high-grade human malignant astrocytomas before and after xenografting into aspiration pockets in rat cortex. human-specific p185c-neu-positive cells were found in tumor specimens from all grades of astrocytoma. significantly fewer p185c-neu-positive cells we ... | 1993 | 8093625 |
| localization of tyrosine hydroxylase in neuronal targets and efferents of the area postrema in the nucleus tractus solitarii of the rat. | catecholamines in the nucleus tractus solitarii (nts) have been implicated in autonomic responses to circulating hormones that act on neurons in the area postrema, the most caudal circumventricular organ in brain. we combined immunoperoxidase labeling of the anterograde tracer, phaseolus vulgaris leucoagglutinin (phal) with immunogold-silver labeling of tyrosine hydroxylase to determine whether this enzymatic marker for catecholamines was present in efferents from the area postrema or their targ ... | 1993 | 8096227 |
| luteinizing hormone-releasing hormone and gamma-aminobutyric acid neurons in the medial preoptic area are synaptic targets of dopamine axons originating in anterior periventricular areas. | the aim of this study was to characterize further the transmitter content and the location of the parent cells of tyrosine hydroxylase-immunoreactive boutons terminating on luteinizing hormone-releasing hormone- and glutamic acid decarboxylase-immunoreactive neurons in the rat medial preoptic area. electron microscopic immunostaining for luteinizing hormone-releasing hormone, tyrosine hydroxylase or glutamic acid decarboxylase was performed on desipramine-pretreated (to protect norepinephrine an ... | 1993 | 8097941 |
| how is leghemoglobin involved in peribacteroid membrane degradation during nodule senescence? | an increase in the rate of succinate and glutamate uptake by isolated symbiosomes from french bean nodules was observed in the presence of iron plus h2o2. the lipid bilayer, and not proteins involved in transport, seems to be the major target of radical attack. leghemoglobin in the presence of a 6-fold excess of h2o2 (where heme breakdown and iron release occurred) provoked also an increase in peribacteroid membrane permeability. in contrast, this hemoprotein in the presence of a 2-fold excess o ... | 1993 | 8100785 |
| the sites of origin of dopaminergic afferent fibers to the lateral habenular nucleus in the rat. | the lateral habenular nucleus of the rat contains a dense plexus of dopaminergic fibers, which are more marked in the medial part of the lateral habenular nucleus than in its lateral counterpart. employing a combination of fluorescent retrograde axonal tracing with fluorogold and tyrosine hydroxylase immunofluorescence histochemistry, we investigated the distribution of cells of origin of the dopaminergic afferent fibers to the lateral habenular nucleus in the rat. the cells double-labeled with ... | 1993 | 8101849 |
| spinal cord and trigeminal projections to the pontine parabrachial region in the rat as demonstrated with phaseolus vulgaris leucoagglutinin. | in order to determine the regions within the parabrachial nucleus that receive synaptic input from nociceptive regions of the spinal cord and medulla in the rat, we analyzed the "golgi-like" labeling produced by anterograde transport of phaseolus vulgaris leucoagglutinin (pha-l) from discrete iontophoretic injections confined to either the superficial dorsal horn of the lumbar spinal cord or to the superficial dorsal horn of the trigeminal nucleus at the level of the obex. labeled fibers from bo ... | 1994 | 8106661 |
| patterns of sialylation in differentiating rat decidual cells as revealed by lectin histochemistry. | lectin histochemistry was used to demonstrate changes in the surface glycan distribution of uterine stromal cells as they differentiate to form decidual cells. decidualization was induced in hormone-treated, ovariectomized rat uteri by needle scratch. uterine tissue from days 2 to 8 of deciduoma development was examined with a panel of lectins specific for terminal non-reducing structures in n- and o-linked classes of glycoprotein glycan, including alpha 2,3- and alpha 2,6-linked sialic acid res ... | 1993 | 8107049 |
| a mutation in the indole-3-acetic acid biosynthesis pathway of pseudomonas syringae pv. syringae affects growth in phaseolus vulgaris and syringomycin production. | homologs of the genes for indole-3-acetic acid (iaa) biosynthesis from pseudomonas syringae pv. savastanoi were retrieved from a genomic library of p. syringae pv. syringae, and their nucleotide sequences were determined. sequence relatedness between the p. syringae pv. syringae and p. syringae pv. savastanoi iaa operons is greater than 90% within the iaam and iaah loci but declines dramatically at a position approximately 200 bp 5' of the iaam translation initiation codon. a third open reading ... | 1994 | 8113177 |
| characterization of a soybean beta-conglycinin-degrading protease cleavage site. | protease c1, an enzyme from soybean (glycine max [l.] merrill cv amsoy 71) seedling cotyledons, was previously determined to be the enzyme responsible for the initial degradation of the alpha' and alpha subunits, but not the beta subunit, of beta-conglycinin storage protein. the sizes of the proteolytic products generated by the action of protease c1 suggest that the cleavage sites on the alpha' and alpha subunits of beta-conglycinin may be located in their n-terminal domain, which is not found ... | 1994 | 8115542 |
| migration of human malignant astrocytoma cells in the mammalian brain: scherer revisited. | fresh suspensions of human glioblastoma multiforme were preincubated in the plant lectin phaseolus vulgaris leucoagglutinin (phal) and implanted into cortical pockets in adult rat brain. brains were investigated periodically over 30 postoperative days and the migration of the human glioblastoma cells was traced with anti-phal immunofluorescence or the overexpression of human specific p185c-neu a specific marker of a class of human malignant astrocytoma cells. the principal pathway of migration o ... | 1993 | 8116480 |
| food properties affecting the digestion and absorption of carbohydrates. | carbohydrate foods differ considerably in their effects on postprandial glucose and insulin responses. qualitative differences among starchy foods are particularly intriguing because of the dominance of starch in human diets. this paper focuses on food properties in cereal (eg, pasta, bread, arepas, and porridge) and legume products (eg, red kidney beans and lentils) that affect metabolic responses to starch. studies in healthy subjects have found that postprandial blood glucose and insulin resp ... | 1994 | 8116553 |
| [nutrition disorders in fattening pigs in an "organic" facility]. | in a herd of fattening pigs under an organic system of management, the following problems were observed: sudden deaths, skin diseases, diarrhea, increased restlessness and cannibalism. the diet consisted of cereals (barley, wheat, rye), field beans, lime stone and deteriorated, raw potatoes without addition of a mineral mixture. deficiencies of sodium, zinc and selenium were found in the diet and in the animals (blood samples, autopsy). the vitamin e status in the pigs was also below normal. | 1993 | 8122238 |
| anterograde and retrograde tracing of projections from the ventral tegmental area to the hippocampal formation in the rat. | employing anterograde tracing with phaseolus vulgaris-leucoagglutinin (pha-l), and a triple labeling protocol using retrogradely transported fluorescent tracers, we examined the projections from the ventral tegmental area (vta-a10) to the hippocampal formation (hf) in the rat. injections of pha-l into vta resulted in labeling in the ventral subiculum (stratum oriens and molecular layer) and in the adjacent ca1 field (stratum oriens, pyramidal, suprapyramidal and molecular layers) of hf. addition ... | 1994 | 8124582 |
| mutation of asn128 to asp of phaseolus vulgaris leucoagglutinin (pha-l) eliminates carbohydrate-binding and biological activity. | phytohaemagglutinin (pha) is the major lectin present in the seeds of the common bean, phaseolus vulgaris, and pha-l is the leucocyte-agglutinating form of this lectin. this tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. based on amino acid sequence comparisons of legume lectins and the three-dimensional structure of lectin-carbohydrate complexes, asn128 can be identified as a likely candidate for site-directed mutagenesis to create a mutant pha-l that does not ... | 1993 | 8130390 |
| embryonic purkinje cells grafted on the surface of the adult uninjured rat cerebellum migrate in the host parenchyma and induce sprouting of intact climbing fibres. | by grafting solid pieces of cerebellar anlage onto the surface of the adult rat cerebellum, we have investigated the problem of the interactions between embryonic and adult neurons in an intact brain. a few days after grafting, embryonic astrocytic processes crossed the graft--host interface and radiated into the recipient molecular layer. several grafted purkinje cells also migrated into the host brain along such processes as well as adult bergmann glia. adult climbing fibres, labelled by means ... | 1994 | 8130928 |
| expression of beta 1-6-branched n-linked oligosaccharides is associated with activation in human t4 and t8 cell populations. | activation of human t lymphocytes by phorbol 12-myristate 13-acetate and leukoagglutinin from phaseolus vulgaris (l-pha) results in important changes in n-glycosylation. the most important event is the increase, in both t4 and t8 cells (especially the latter), of l-pha+ structures characterized by beta 1-6-branching of complex-type oligosaccharides. moreover, the existence of a cd4-mediated increase of these beta 1-6-branched structures on positively selected t4 cells, as compared with the negat ... | 1994 | 8132531 |
| genital lesions following long-term administration of clenbuterol in female pigs. | pathologic findings, lectin histochemistry, and nuclear estrogen receptors were studied in the reproductive organs of gilts treated with clenbuterol. a ration containing 1 ppm of clenbuterol was fed for 40 days to four landrace x large white, 9-month-old gilts, weighing 134 to 172 kg at slaughter (gilt nos. 5-8). four gilts (nos. 1-4) served as controls. treated animals had macroscopic lesions characterized by microcystic ovaries and uterine atrophy. histopathologic lesions included atretic dege ... | 1994 | 8140731 |
| in vitro labeling strategies for identifying primary neural tissue and a neuronal cell line after transplantation in the cns. | potential labels for identifying embryonic raphe neurons and a clonal, neuronally differentiating, raphe-derived cell line, rn33b, in cns transplantation studies were tested by first characterizing the labels in vitro. the labels that were tested included 4',6-diamidino-2-phenylindole hydrochloride, 1,1'-dioctadecyl-3,3,3'-tetramethylindocarbocyanine perchlorate, the escherichia coli lacz gene, fast blue, fluoro-gold, fluorescein-conjugated latex microspheres, fluorescein isothiocyanate-conjugat ... | 1993 | 8143080 |
| a phaseolus lectin anterograde tracing study of the tectorotundal projections in the domestic chick. | the projection from the chick optic tectum to the nucleus rotundus was investigated by iontophoresis of the anterograde tracer phaseolus vulgaris lectin (pha-l) into the optic tectum and by golgi impregnation. pha-l labelled fibres were observed within the ipsilateral nucleus rotundus and to course through the supraoptic decussation to the contralateral nucleus rotundus. they appeared similar to fibres evident in golgi impregnated material. before reaching the nucleus rotundus, pha-l labelled fi ... | 1994 | 8157485 |
| the cloning and characterization of phage promoters, directing high expression of luciferase in pseudomonas syringae pv. phaseolicola, allowing single cell and microcolony detection. | regions of dna containing promoter sequences from a pseudomonas syringae pv. phaseolicola-specific phage (phi 11p) were identified by shotgun cloning into a broad-host-range promoter-probe vector (pqf70). when used in conjunction with the luciferase reporter genes, one of these dna fragments, 19h, directed gene expression at a level which enabled the subsequent light output (bioluminescence) of single cells of p. syringae pv. phaseolicola to be detected and visualized using a charge-coupled devi ... | 1993 | 8162227 |
| nodulating ability of rhizobium tropici is conditioned by a plasmid-encoded citrate synthase. | rhizobium species elicit the formation of nitrogen-fixing root nodules through a complex interaction between bacteria and plants. various bacterial genes involved in the nodulation and nitrogen-fixation processes have been described and most have been localized on the symbiotic plasmids (psym). we have found a gene encoding citrate synthase on the psym plasmid of rhizobium tropici, a species that forms nitrogen-fixing nodules on the roots of beans (phaseolus vulgaris) and trees (leucaena spp.). ... | 1994 | 8170393 |
| projections of the dorsal raphe nucleus to the brainstem: pha-l analysis in the rat. | early studies that used older tracing techniques reported exceedingly few projections from the dorsal raphe nucleus (dr) to the brainstem. the present report examined dr projections to the brainstem by use of the anterograde anatomical tracer phaseolus vulgaris leucoagglutinin (pha-l). dr fibers were found to terminate relatively substantially in several structures of the midbrain, pons, and medulla. the following pontine and midbrain nuclei receive moderate to dense projections from the dr: pon ... | 1994 | 8176000 |
| glycosylation pattern and enzyme activities in atrophic, angulated skeletal muscle fibres from ageing rats. | in this study enzyme activities and lectin binding patterns in skeletal muscle from very old rats were investigated in order to evaluate changes in enzyme activity or carbohydrate expression in senile muscle. activities for adenosine triphosphatase (atpase), succinic dehydrogenase, non-specific esterase and the binding pattern for 31 lectins were investigated in the soleus muscles from very old (36 months) and young (3 months) rats. in ageing muscles atrophic, angulated muscle fibres are frequen ... | 1994 | 8186892 |
| direct projections from the non-laminated divisions of the medial geniculate nucleus to the temporal polar cortex and amygdala in the cat. | the medial geniculate nucleus (mg) is well known to send projection fibers not only to the auditory cortex, but also to the limbic structures of the forebrain including the perirhinal cortex and amygdala. in the cat, the non-laminated portions of the mg are also known to project to the amygdala, as well as to the auditory cortical areas surrounding the primary auditory area. on the other hand, projections from the non-laminated mg to the limbic cortical areas have not so far been studied systema ... | 1994 | 8188859 |
| efferent connections from the lateral hypothalamic region and the lateral preoptic area to the hypothalamic paraventricular nucleus of the rat. | the lateral preoptic and lateral hypothalamic regions contain the majority of the cell groups embedded in the fibre trajectories of the medial forebrain bundle on its course through the hypothalamus. recent studies have extended considerably the parcellation of the lateral hypothalamic region, and therefore, the need to emphasize new insights into the anatomical organisation of projections from the neurons of the lateral hypothalamic region. in the present study we describe the anatomical organi ... | 1994 | 8201036 |
| in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. | the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ... | 1994 | 8206443 |
| heterologous encapsidation of recombinant pea early browning virus. | the coat protein gene of pea early browning virus (pebv) was replaced with that of another tobravirus, tobacco rattle virus (trv strain ppk20). the recombinant virus multiplied efficiently in the systemic host nicotiana benthamiana and, on the local lesion host phaseolus vulgaris, produced symptoms typical of pebv rather than trv showing that viral coat protein is not a determinant for lesion morphology. both viral rnas were encapsidated by trv coat protein although the shorter particles (encaps ... | 1994 | 8207406 |
| several neuronal and axonal types form long intrinsic connections in the cat primary auditory cortical field (ai). | intrinsic connections in the cat primary auditory field (ai) as revealed by injections of phaseolus vulgaris leucoagglutinin (pha-l) or biocytin, had an anisotropic and patchy distribution. neurons, labelled retrogradely with pha-l were concentrated along a dorsoventral stripe through the injection site and rostral to it; the spread of rostrally located neurons was greater after injections into regions of low rather than high characteristic frequencies. the intensity of retrograde labelling vari ... | 1993 | 8214628 |
| neuroanatomical study of afferent projections to the supramammillary nucleus of the rat. | we examined the regions projecting to the supramammillary nucleus of the rat with retrograde transport of wga-hrp and wga, and anterograde transport of phaseolus vulgaris leucoagglutinin. the supramammillary nucleus receives major descending afferents from the infralimbic cortex, the dorsal peduncular cortex, the nucleus of the diagonal band of broca, the medial and lateral preoptic nuclei, bilaterally. the major ascending afferents come from the pars compacta of the nucleus centralis superior, ... | 1993 | 8214629 |
| predictions of protein digestibility and lysine absorption in pigs. | with 294 feedstuffs-classified into 22 feedstuff categories-, from which in the original literature besides the crude protein (cp) and lysine (lys) contents their precaecal (pc) and partly their total (t) digestibilities in pigs had been presented, assessments of the pc and t apparent digestibilities (ad%) were done by two different ways. between the prediction possibility using the mean value of nondigestible cp resp. lys (mean ndcp, means ndlys, g/kg dm) and the usual way of calculating averag ... | 1993 | 8215881 |
| activation of a tobacco glycine-rich protein gene by a fungal glucan preparation. | a phytophthora megasperma f.sp. glycinea cell wall glucan preparation was previously shown to protect tobacco plants against viral infection. eleven plant defense-related genes were assayed for elevated mrna accumulation levels in response to glucan treatment of tobacco plants. the expression of only one of these genes, a glycine-rich protein (grp) gene, was induced by glucan application. elevated grp gene mrna levels could be detected within 15 min of glucan treatment and reached maximum levels ... | 1993 | 8220493 |
| higher plant mitochondria encode an homologue of the nuclear-encoded 30-kda subunit of bovine mitochondrial complex i. | we describe the structure and expression of a wheat mitochondrial gene, which codes for a subunit of mitochondrial nadh dehydrogenase. the deduced protein sequence has 70% similarity to the 30-kda subunit of bovine mitochondrial complex i and 65% similarity to the 31-kda subunit of neurospora crassa complex i, components of the iron-sulfur-protein fraction, both nuclear-encoded proteins. we named this wheat mitochondrial gene as nad9. the wheat nad9 gene is transcribed in a single mrna of 0.9 kb ... | 1993 | 8223639 |
| demonstration of feline and canine platelet glycoproteins by immuno- and lectin histochemistry. | canine and feline platelet cytocentrifuge preparations (ccps), cryostat and paraffin-embedded bone marrow sections were used in this study. we evaluated whether platelets, megakaryocytes and megakaryocyte precursor cells could be labelled by monoclonal antibodies (y2/51, clb-thromb/1, hpl1) against human platelet membrane glycoprotein gp iiia and the gp iib/iiia complex or by the following 10 biotinylated lectins: concanavalin a (con a), lens culinaris agglutinin (lca), pisum sativum agglutinin ... | 1993 | 8226111 |
| kidney bean lectin-induced escherichia coli overgrowth in the small intestine is blocked by gna, a mannose-specific lectin. | the reversible and dose-dependent hyperplastic growth of the small intestine and accelerated epithelial cell turnover caused by feeding rats with diets containing kidney bean lectin (pha) increased the proportion of immature cells on the villi whose membrane and/or cytoplasm contained mainly simple, polymannosylated glycans. these new alpha-linked mannosyl terminals, particularly of the damaged epithelium, facilitated the preferential adherence of opportunistic escherichia coli with mannose-sens ... | 1993 | 8226393 |
| relationship of descending inferior colliculus projections to olivocochlear neurons. | with the objective of defining the relationship of descending inferior colliculus projections to the olivocochlear system in the guinea pig, inferior colliculus neurons were anterogradely labeled with phaseolus vulgaris-leucoagglutinin and olivocochlear neurons were retrogradely labeled with horseradish peroxidase in the same brain sections. inferior colliculus neurons were found to project to many nuclei and regions of the hindbrain where olivocochlear neurons reside. the most substantial of th ... | 1993 | 8227527 |
| spinal termination patterns of canine identified a-delta and c spermatic polymodal receptors traced by intracellular labeling with phaseolus vulgaris-leucoagglutinin. | the spinal projection patterns of spermatic polymodal receptors were studied by intracellular labeling of functionally identified canine dorsal root ganglion (drg) neurons with phaseolus vulgaris-leucoagglutinin (pha-l). the processes of 2 c-fiber and 1 a-delta-fiber spermatic polymodal receptor neurons were labeled well enough to trace their central terminations. the labeled c-fiber drg neurons were of medium size (mean diameter 36.8 and 40.7 microns). on entering the spinal cord, axons of the ... | 1993 | 8227530 |
| miso from peas (pisum sativum) and beans (phaseolus vulgaris) of domestic origin. fermented foods from agricultural products in europe. ii. | miso is a fermented soybean paste widely used in japan as a soup base or as a seasoning agent. koji (cereal grains with the mold aspergillus oryzae) serves as enzyme source. peas (pisum sativum) and beans (phaseolus vulgaris) of german origin can be used as substitutes for soybeans in the preparation of miso-like products. the legumes (peas, beans and soybeans for comparison) are washed, soaked in boiled water, dehulled and cooked for 35 min at reduced pressure. after grounding the seeds are mix ... | 1993 | 8237083 |
| genomic heterogeneity among french rhizobium strains isolated from phaseolus vulgaris l. | levels of dna relatedness between strains isolated from root nodules of phaseolus vulgaris and reference strains of different rhizobium species were determined by performing dna-dna hybridization experiments (s1 nuclease method). the nine strains examined were members of three genomic groups previously delineated by a restriction fragment length polymorphism analysis among strains isolated from p. vulgaris at different sites in france. in agreement with the results of the restriction fragment le ... | 1993 | 8240956 |
| isolation of an immunosuppressive lectin from phaseolus vulgaris l. cv cacahuate using stroma. | an immunosuppressive lectin was isolated from seed of phaseolus vulgaris cv cacahuate using physically entrapped stroma. the lectin was found to be a 94 kda tetrameric protein. when 50 micrograms, of this lectin were administered intraperitoneally 2 days before the immunization with sheep red blood cells, humoral response against the immunogen was completely inhibited. other properties of the protein are discussed. | 1993 | 8248029 |
| immunohistochemical and lectin dissection of the human nephron in health and disease. | many renal diseases involving the tubular epithelium appear to preferentially affect certain nephron segments. while major portions of the nephron, such as proximal and distal convoluted tubules and collecting ducts, can be identified in the normal kidney, the distinction of diseased nephron segments can be difficult in tissue sections. thus, to identify which nephron segments are involved in pathologic changes is usually impossible by routine histologic examination alone. recently antibody and ... | 1993 | 8250694 |
| the effect of graded inclusion of baked beans (phaseolus vulgaris) on plasma and liver lipids in hypercholesterolaemic pigs given a western-type diet. | the aim of the present study was to measure the effect of graded inclusion of baked beans (phaseolus vulgaris) on plasma and liver lipids in hypercholesterolaemic pigs fed on a western-type diet. twenty-four large white x landrace pigs of about 30 kg body weight were made hypercholesterolaemic by feeding a semi-purified diet, high in saturated fat and supplemented with 10 g cholesterol/kg, for 14 d. after that, six pigs were randomly assigned to one of the four experimental groups. they received ... | 1993 | 8260478 |
| nascent transcript-binding protein of the pea chloroplast transcriptionally active chromosome. | this study describes the nascent rna-binding protein of the pea chloroplast transcriptional complex. the protein has been identified by photoaffinity labelling of the transcriptionally active chromosome (tac) which utilizes the endogenous plastid dna as template. uv irradiation of lysed chloroplast or the isolated tac under conditions optimized for transcription photocross-links nascent radiolabelled transcripts (up to 250 nucleotides in length) to a 48 kda protein. the photoaffinity labelling o ... | 1993 | 8260634 |
| exposure of the rat small intestine to raw kidney beans results in reorganization of absorptive cell microvilli. | a single exposure to raw kidney beans (rkb) results in vesiculation, shortening, and then regrowth of microvilli in the rat small intestine. this study investigated changes that occur in the structure of microvilli 2-10 hours after rkb exposure. | 1994 | 8276211 |