| phenotypic and genomic comparison of mycobacterium aurum and surrogate model species to mycobacterium tuberculosis: implications for drug discovery. | tuberculosis (tb) is caused by mycobacterium tuberculosis and represents one of the major challenges facing drug discovery initiatives worldwide. the considerable rise in bacterial drug resistance in recent years has led to the need of new drugs and drug regimens. model systems are regularly used to speed-up the drug discovery process and circumvent biosafety issues associated with manipulating m. tuberculosis. these include the use of strains such as mycobacterium smegmatis and mycobacterium ma ... | 2017 | 28705154 |
| detection of mycobacterium marinum, m. peregrinum, m. fortuitum and m. abscessus in aquarium fish. | | 2017 | 28699669 |
| fish pedicure-induced foot mycobacteriosis infection due to mycobacterium marinum: a first case report. | | 2017 | 28677579 |
| a small-molecule allosteric inhibitor of mycobacterium tuberculosis tryptophan synthase. | new antibiotics with novel targets are greatly needed. bacteria have numerous essential functions, but only a small fraction of such processes-primarily those involved in macromolecular synthesis-are inhibited by current drugs. targeting metabolic enzymes has been the focus of recent interest, but effective inhibitors have been difficult to identify. we describe a synthetic azetidine derivative, brd4592, that kills mycobacterium tuberculosis (mtb) through allosteric inhibition of tryptophan synt ... | 2017 | 28671682 |
| mycobacterium marinum and carpal tunnel syndrome: three case reports. | infection with mycobacterium marinum is often difficult to diagnose. infection with m. marinum in the upper extremity may involve the tendon sheaths, producing clinical manifestations such as tenosynovitis and symptoms of carpal tunnel syndrome. we report 3 cases of m. marinum infection of the hand associated with carpal tunnel syndrome during an outbreak in new york city's chinatown. a combination of carpal tunnel release, flexor tenosynovectomy, and appropriate antibiotics yielded complete res ... | 2017 | 28669531 |
| the brief case: a fishy tale prevents digital doom following polly's peck-the importance of pets in a comprehensive medical history. | | 2017 | 28646087 |
| outbreak of non-tuberculous mycobacteria skin or soft tissue infections associated with handling fish - new york city, 2013-2014. | mycobacterium marinum, a bacterium found in freshwater and saltwater, can infect persons with direct exposure to fish or aquariums. during december 2013, the new york city department of health and mental hygiene learned of four suspected or confirmed m. marinum skin or soft tissue infections (sstis) among persons who purchased whole fish from chinese markets. ninety-eight case-patients with non-tuberculous mycobacteria (ntm) sstis were identified with onset june 2013-march 2014. of these, 77 (79 ... | 2017 | 28629484 |
| β-ca-specific inhibitor dithiocarbamate fc14-584b: a novel antimycobacterial agent with potential to treat drug-resistant tuberculosis. | inhibition of novel biological pathways in mycobacterium tuberculosis (mtb) creates the potential for alternative approaches for treating drug-resistant tuberculosis. in vitro studies have shown that dithiocarbamate-derived β-carbonic anhydrase (β-ca) inhibitors fc14-594 a and fc14-584b effectively inhibit the activity of mtb β-ca enzymes. we screened the dithiocarbamates for toxicity, and studied the in vivo inhibitory effect of the least toxic inhibitor on m. marinum in a zebrafish model. in o ... | 2017 | 28629306 |
| ohmyungsamycins promote antimicrobial responses through autophagy activation via amp-activated protein kinase pathway. | the induction of host cell autophagy by various autophagy inducers contributes to the antimicrobial host defense against mycobacterium tuberculosis (mtb), a major pathogenic strain that causes human tuberculosis. in this study, we present a role for the newly identified cyclic peptides ohmyungsamycins (oms) a and b in the antimicrobial responses against mtb infections by activating autophagy in murine bone marrow-derived macrophages (bmdms). oms robustly activated autophagy, which was essentiall ... | 2017 | 28611371 |
| whib4 regulates the pe/ppe gene family and is essential for virulence of mycobacterium marinum. | during the course of infection, pathogenic mycobacteria including mycobacterium tuberculosis (m. tb) encounter host environments of variable oxygen tension, ranging from the hypoxic center of granulomas to the most oxygenated region in the lung cavities. mycobacterial responses to changes of oxygen tension are critically related to infection outcomes, such as latency and reactivation. whib4 is an iron-sulfur containing transcription factor that is highly sensitive to oxygen exposure. in this stu ... | 2017 | 28592799 |
| two cases of mycobacterium marinum infection on the upper limbs. | | 2017 | 28556948 |
| characterization of mycobacterium marinum infections in zebrafish wounds and sinus tracts. | the impaired healing of mycobacterium tuberculosis-infected wounds is a clinical challenge, and the mechanisms involved are still not clear. the zebrafish model of mycobacterium marinum infection has provided surprising insights into the pathogenesis of tuberculosis in humans. similarly, the major principles and phases of cutaneous wound healing are conserved among adult mammals and adult zebrafish. here, we injected mycobacterium marinum into the dorsal muscles of adult zebrafish and observed t ... | 2017 | 28466489 |
| mycobacterium marinum antagonistically induces an autophagic response while repressing the autophagic flux in a torc1- and esx-1-dependent manner. | autophagy is a eukaryotic catabolic process also participating in cell-autonomous defence. infected host cells generate double-membrane autophagosomes that mature in autolysosomes to engulf, kill and digest cytoplasmic pathogens. however, several bacteria subvert autophagy and benefit from its machinery and functions. monitoring infection stages by genetics, pharmacology and microscopy, we demonstrate that the esx-1 secretion system of mycobacterium marinum, a close relative to m. tuberculosis, ... | 2017 | 28414774 |
| clinical implications for the timely diagnosis of mycobacterium marinum in the age of biologic therapy: a case report and review of the literature. | mycobacterium marinum infections typically present as cutaneous nodular lesions with a sporotrichoid lymphatic spread on extensor surfaces of extremities. the natural history of this infection can be altered if the host is immunosuppressed, leading to disseminated presentations. a detailed exposure history and high degree of suspicion for this indolent pathogen are often required for the correct diagnosis of this disease. we present a case of a 67-year-old male misdiagnosed with seronegative rhe ... | 2017 | 28392952 |
| mycobacterium marinum infection of the hand in an immunocompromised aquarium hobbyist. | m. marinum, a nontuberculous mycobacterium, is a rare human pathogen widely distributed in the aquatic environment. in the previous century, epidemics took place due to inadequately chlorinated swimming pool water. nowadays the majority of infections are acquired through contact of previously damaged skin with contaminated fish tank water. we present a case of m. marinum infection of the hand in an aquarium hobbyist which stayed unrecognized for 2 years. after confirming the correct diagnosis, t ... | 2017 | 28511753 |
| cyclophostin and cyclipostins analogs, new promising molecules to treat mycobacterial-related diseases. | the progression of mycobacterial diseases requires the development of new therapeutics. here, we evaluated the efficacy and selectivity of a panel of cyclophostin and cyclipostins analogs (cycs) against various bacteria and mycobacteria. the activity of these 26 cycs was first assayed using the agar plate method. compounds exhibiting a 50-100% growth inhibition rate were then selected to determine their mic using the rema assay. the best drug candidate was further tested against mycobacterial cl ... | 2017 | 29241819 |
| structural and functional characterisation of the cytochrome p450 enzyme cyp268a2 from mycobacterium marinum. | members of the cytochrome p450 monooxygenase family cyp268 are found across a broad range of mycobacterium species including the pathogens mycobacterium avium, m. colombiense,m. kansasii and mmarinum cyp268a2, from m. marinum , which is the first member of this family to be studied, was purified and characterised. cyp268a2 was found to bind a variety of substrates with high affinity, including branched and straight-chain fatty acids (c10-c12), acetate esters, and aromatic compounds. the enzyme w ... | 2018 | 29343612 |
| mycobacterium marinum infection in zebrafish and microglia imitates the early stage of tuberculous meningitis. | mycobacterium tuberculosis (m. tuberculosis) invading and activating microglia causes the most serious subtypes of tuberculosis called tubercular meningitis. however, the developmental process of tubercular meningitis, especially the early phase, is poorly understood due to lacking well-established and well-accepted visible models in vitro and in vivo. here, consistent with one recent report, we found mycobacterium marinum (m. marinum) invade the zebrafish brain and subsequently cause granuloma- ... | 2018 | 29352446 |
| using rt qpcr for quantifying mycobacteria marinum from in vitro and in vivo samples. | mycobacterium marinum, the causative agent of fish tuberculosis, is rarely a human pathogen causing a chronic skin infection. it is now wildely used as a model system in animal models, especially in zebra fish model, to study the pathology of tuberculosis and as a means of screening new anti-tuberculosis agent. to facilitate such research, quantifying the viable count of m. marinum bacteria is a crucial step. the main approach used currently is still by counting the number of colony forming unit ... | 2018 | 29322466 |
| survey on medicinal plants traditionally used in senegal for the treatment of tuberculosis (tb) and assessment of their antimycobacterial activity. | in west africa, populations are used to taking traditional medicine as a first aid against common health problems. in this aspect, many plants are claimed to be effective in the treatment of tuberculosis (tb), which according to the world health organization (who) remains one of the world's deadliest communicable diseases. | 2017 | 29289797 |
| cell envelope stress in mycobacteria is regulated by the novel signal transduction atpase inir in response to trehalose. | the cell envelope of mycobacteria is a highly unique and complex structure that is functionally equivalent to that of gram-negative bacteria to protect the bacterial cell. defects in the integrity or assembly of this cell envelope must be sensed to allow the induction of stress response systems. the promoter that is specifically and most strongly induced upon exposure to ethambutol and isoniazid, first line drugs that affect cell envelope biogenesis, is the inibac promoter. in this study, we set ... | 2017 | 29281637 |
| acute infection due to mycobacterium marinum in a patient with ulcerative colitis and metabolic syndrome on infliximab therapy. | | 2017 | 29258704 |
| buruli ulcer, a prototype for ecosystem-related infection, caused by mycobacterium ulcerans. | buruli ulcer is a noncontagious disabling cutaneous and subcutaneous mycobacteriosis reported by 33 countries in africa, asia, oceania, and south america. the causative agent, mycobacterium ulcerans, derives from mycobacterium marinum by genomic reduction and acquisition of a plasmid-borne, nonribosomal cytotoxin mycolactone, the major virulence factor. m. ulcerans-specific sequences have been readily detected in aquatic environments in food chains involving small mammals. skin contamination com ... | 2018 | 29237707 |
| mycobacterium marinum remains an unrecognized cause of indolent skin infections. | we identified 5 patients who had cutaneous lesions with cultures that yielded mycobacterium marinum. it was discovered that all 5 patients had a home aquarium, and infection was preceded by trauma to the hand. however, the association between the development of the infection and exposure of the trauma site to the aquarium was not initially established until repeated questioning was performed. skin biopsies or incision and drainage were performed for all patients, and the diagnosis was establishe ... | 2017 | 29232419 |
| [mycobacterium marinum infection: report of a case]. | | 2017 | 29224287 |
| priming of innate antimycobacterial immunity by heat-killed listeria monocytogenes induces sterilizing response in the adult zebrafish tuberculosis model. | mycobacterium tuberculosis remains one of the most problematic infectious agents due to its highly developed mechanisms to evade host immune responses combined with the increasing emergence of antibiotic resistance. host-directed therapies aiming at optimal modulation of immune responses to improve bacterial eradication or to limit excessive inflammation are a new strategy for the treatment of tuberculosis. in this study, we have established a zebrafish-mycobacterium marinum natural host-pathoge ... | 2017 | 29208761 |
| eczema-like fish tank granuloma: a new clinical presentation of mycobacterium marinum infection. | | 2017 | 29194791 |
| severe inhibition of lipooligosaccharide synthesis induces tlr2-dependent elimination of mycobacterium marinum from thp1-derived macrophages. | although mycobacterial glycolipids are among the first-line molecules involved in host-pathogen interactions, their contribution in virulence remains incomplete. mycobacterium marinum is a waterborne pathogen of fish and other ectotherms, closely related to mycobacterium tuberculosis. since it causes tuberculosis-like systemic infection it is widely used as a model organism for studying the pathogenesis of tuberculosis. it is also an occasional opportunistic human pathogen. the m. marinum surfac ... | 2017 | 29183333 |
| whib6 regulation of esx-1 gene expression is controlled by a negative feedback loop in mycobacterium marinum. | esx (esat-6 system) export systems play diverse roles across mycobacterial species. interestingly, genetic disruption of esx systems in different species does not result in an accumulation of protein substrates in the mycobacterial cell. however, the mechanisms underlying this observation are elusive. we hypothesized that the levels of esx substrates were regulated by a feedback-control mechanism, linking the levels of substrates to the secretory status of esx systems. to test this hypothesis, w ... | 2017 | 29180415 |
| intra-amoebal killing of mycobacterium ulcerans by acanthamoeba griffini: a co-culture model. | mycobacterium ulcerans, a decaying mycobacterium marinum derivative is responsible for buruli ulcer, a notifiable non-contagious disabling infection highly prevalent in some west african countries. aquatic environments are suspected to host m. ulcerans, however, the exact reservoirs remain unknown. while m. marinum was found to resist amoebal microbicidal activities, this remains unknown for m. ulcerans. in this study m. ulcerans was co-cultured with the moderately halophile acanthamoeba griffin ... | 2017 | 29155010 |
| [skin infection due to mycobacterium marinum]. | | 2017 | 29132844 |
| two cases of occupationally acquired mycobacterium marinum infection in chinese restaurant workers in melbourne, australia. | | 2017 | 29076528 |
| local hyperthermia therapy for refractory cutaneous mycobacterium marinum infection. | | 2017 | 29028142 |
| an unusual cutaneous infection caused by mycobacterium marinum. | introduction.mycobacterium marinum is a non-tubercular mycobacterium residing in fresh or salt water (in tropical or temperate areas); it is a fish and human pathogen, and in immunocompromised patients can cause severe cutaneous and subcutaneous infections. case presentation. a 46-year-old white man who underwent immunosuppressive therapy was admitted to our department in may 2016 for skin lesions previously diagnosed as 'unusual erysipelas'. we rejected the hypothesis of erysipelas, due to the ... | 2017 | 29026615 |
| partners in crime: phenolic glycolipids and macrophages. | two recent articles advance our understanding of mycobacterial pathogenesis, revealing key roles for bacterially derived phenolic glycolipids (pgls). in leprosy, mycobacterium leprae pgl-1 uniquely subverts local macrophages to produce neurotoxic nitric oxide (no), leading to nerve demyelination. in a related model, mycobacterium marinum pgl stimulates the recruitment of growth-conducive monocytes to sites of initial infection as an early immune evasion strategy. | 2017 | 28969977 |
| a fluorescence-based reporter for monitoring expression of mycobacterial cytochrome bd in response to antibacterials and during infection. | cytochrome bd is a component of the oxidative phosphorylation pathway in many gram-positive and gram-negative bacteria. next to its role as a terminal oxidase in the respiratory chain this enzyme plays an important role as a survival factor in the bacterial stress response. in mycobacterium tuberculosis and related mycobacterial strains, cytochrome bd is an important component of the defense system against antibacterial drugs. in this report we describe and evaluate an mcherry-based fluorescent ... | 2017 | 28878275 |
| rational design of drug-like compounds targeting mycobacterium marinum melf protein. | the mycobacterial mel2 locus (mycobacterial enhanced infection locus, rv1936-1941) is mycobacterium marinum and m. tuberculosis specific, which can withstand reactive oxygen species (ros) and reactive nitrogen species (rns) induced stress. a library of over a million compounds was screened using in silico virtual ligand screening (vls) to identify inhibitors against the modeled structure of melf protein expressed by melf of mel2 locus so that m. marinum's ability to withstand ros/rns stress coul ... | 2017 | 28873466 |