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electron microscopic study of membranes and walls of bacteria and changes occurring during growth initiation.thin sections of stationary-phase streptococcus lactis cells showed that the wall and membrane are 20 and 7 nm thick, respectively. whole cells were examined by negative staining with ammonium molybdate and by shadowing. on air-drying of whole cells, the membrane pulled away from the wall revealing adhesions between these organelles. adhesions could not be seen after subculture of the stationary-phase cells into complex media or into solutions containing glucose, kcl, and cacl(2) in tris(hydroxy ...19694180467
location of bacterial polysaccharide during various phases of growth.polysaccharide-containing material was located by histochemical staining on ultrathin sections of bacillus cereus and several clostridial species during various phases of growth. these components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. in b. cereus any carbohydrate-containing material present along the forespore membrane was too small to be detected by direct staining. however, in three clostridial species such material was ...19694182530
quantitative assay of a single proteolytic enzyme in a crude mixture of bacterial proteinases. 19694189511
the location of chemical components on ultrathin sections of bacillus cereus embedded in glycol methacrylate. 19694189833
association of the crystalline inclusion of bacillus thuringiensis with the exosporium.thin sections of sporulating cells of bacillus thuringiensis show a marked association between the crystalline inclusion and the exosporium.19704192566
spores of microorganisms. 23. interdependence of intra- and extra-cellular levels of calcium: its effect on the germination of bacterial spores in different media. 19704193500
the effect of fixation on the staining of the bacterial cross cell wall. 19704194094
kinetics of -lactamase inactivation of penicillins i: effect of side-chain structure, ionic strength, ph, and temperature. 19734196205
rna polymerase from vegetative cells and spores of bacillus cereus t. ii. peptide composition. 19734196221
penicillin-binding component of bacillus cereus.(14)c-penicillin is irreversibly bound by bacillus cereus 569. incubation of penicillin-treated cells in a cell wall digestion medium results in solubilization of approximately 60% of the irreversibly bound lable. the extent of the solubilization is the same when cells are prepared by either a cold or 37 c treatment procedure. however, spheroplasts prepared by the cold treatment are leaky. when the resulting spheroplasts are incubated in supplemented medium, reduced rates and levels of penicilli ...19734196243
outgrowth of bacillus cereus spores harboring bacteriophage cp-51 dna. i. initiation of bacteriophage development.time of initiation of bacteriophage cp-51 rna synthesis, inhibition of host dna synthesis, initiation of cp-51 dna synthesis, and release of phage was determined in infected bacillus cereus cells and outgrowing spores harboring phage dna. the development of phage in outgrowing spores is initiated 45 min after induction of spore germination and then proceeds at a rate similar to that observed in infected cells. although spore rna synthesis is inhibited by the presence of chloramphenicol or puromy ...19734196633
involvement of a carboxyl group in the active site of bacillus cereus 569-h penicillinse ( -lactamase i). 19734197157
inhibition of phosphoenolpyruvate:uridine diphosphate n-acetylglucosamine enolpyruvyltransferase by uridine diphosphate n-acetylmuramyl peptides. 19734197158
characterization and partial purification of -hydroxybutyrate dehydrogenase from sporulating cells of bacillus cereus t. 19734197242
effect of aflatoxins on microorganisms. 19724197365
[toxic properties of the metabolites of some saprophytic bacteria]. 19724197401
the effect of d-l-erythro- - -dihydroxybutyraldehyde (diba) on the spores of bacillus cereus: germination, stimulation and outgrowth inhibition. 19734197475
[study of the antibiotic activity of mycetes isolated from the soil of various regions of bulgaria. i]. 19724197602
induction of biohydrogenation of oleic acid in bacillus cereus by increase in temperature. 19734197961
extracellular factor synthesized by bacillus cereus which evokes a dermal reaction in guinea pigs.a guinea pig skin assay was developed as a measure of ileal loop fluid-inducing factor in the rabbit and possibly of the causative principle of bacillus cereus food poisoning. a green or bloody necrotic reaction was produced in the guinea pig skin by injection of culture filtrates from 21 of 24 b. cereus strains tested, and by no other bacillus species tested except for the closely related b. thuringiensis. the skin factor was synthesized and excreted by logarithmically growing cells, inactivate ...19734198101
environmental factors internal to the host that affect the sucess of microbial insecticides. 19734198128
electron microscopy of bacillus subtilis protoplast membrane after treatment with phospholipase a 2 and phospholipase c. 19734198136
[survival of some terrestrial microorganisms in space]. 19724198265
toxicity of metabolites produced by the "alternaria". 19734198474
tetracycline interference with the bactericidal effect of serum. 19734198604
purification and properties of an extracellular polysaccharide containing amino sugars formed by bacillus cereus. 19734198886
morphological abnormalities of fungi induced by volatile microbial metabolites. 19734199221
release and recovery of forespores from bacillus cereus.a method is described which makes possible the release of immature forespores from sporulating cells at specific stages of development, from the completion of stage iii through to mature spore formation. with the aid of zonal density gradient centrifugation, the method makes possible the recovery of quantities of forespores ample for biochemical and physical studies. with the capability to examine forespores and some mother cell components independently, we have established that several enzymes ...19734199507
exosporium and spore coat formation in bacillus cereus t.the exosporium of bacillus cereus t was first observed as a small lamella in the cytoplasm in proximity to the outer forespore membrane (ofsm) near the middle of the sporangium. serial sections, various staining methods, and enzyme treatments failed to show any connections between the small lamella and the ofsm. the advancing edge of the exosporium moved toward the polar end of the cell until the spore was completely enveloped. the middle coat was formed between the exosporium and the ofsm from ...19734199508
[peracute mastitis caused by bacillus cereus]. 19724199535
[the bacteriology of dehydrated vegetable products]. 19724199536
[studies on the activity of proteolytic enzymes in bacillus anthracis and bacillus cereus]. 19734199573
comparison of bacillus thuringiensis with other bacillus species based on enzymoserological examinations of their proteolytic enzymes. 19734199697
pharmacokinetic studies on minocycline in man. 19734199710
phospholipase c (bacillus cereus) acts only at the inner surface of the erythrocyte membrane. 19734199713
[various phenomena accompanying lethal shock due to bacillus cereus]. 19734199843
[the isoelectric point of extracellular proteases of certain bacterial species]. 19724200183
[ozone for disinfection of water contaminated with vegetative and spore forms of bacteria, fungi, and viruses]. 19734200343
destruction of psychrophilic strains of bacillus by chlorine. 19734200453
development of ultraviolet resistance in sporulating bacillus cereus t.ultraviolet (uv) resistance patterns of sporulating bacillus cereus were determined. six different categories of uv resistance were discernible as the organism progressed from exponential growth to the free, mature spore. light microscope observations allowed the assignment of certain sporulation stages to specific uv resistance categories. marked changes in uv resistance were associated with stages iii, mid-iv, and post-iv of sporulation. dipicolinic acid was shown to sensitize forespores to uv ...19734200858
carbon monoxide production from hydroxocobalamin by bacteria. 19734200896
advances in the treatment of infectious diseases. 19734201148
measurement of heat resistance parameters for spores isolated from canned products. 19734201180
sensitivity of three selected bacterial species to ozone.the minimal lethal concentration of ozone in water was determined for three bacterial species: escherichia coli, bacillus cereus, and bacillus megaterium. a contact period of 5 min was selected. the lethal threshold concentration for the cells of b. cereus was 0.12 mg/liter while that for e. coli and b. megaterium was 0.19 mg/liter. low concentrations of ozone were ineffective when organic matter was present to interfere with the action of ozone on the bacterial cells. also determined during the ...19734201643
germination of spores of bacillus cereus in milk and milk dialysates: effect of heat treatment. 19734201828
screening and isolation of penicillinase inhibitor, ka-107.it is known that penicillin resistance of bacteria is mainly caused by the inactivation of penicillin by penicillinase derived from such strains. we have developed a screening procedure for penicillinase inhibitors. several microorganisms were found to produce such inhibitors, and from the culture filtrate of streptomyces gedanensis atcc 4880 a penicillinase inhibitor, named ka-107, was isolated. the characteristics of this inhibitor were revealed by an in vitro test by using penicillinase deriv ...19734202340
in vitro stimulation of lecithin synthesis in rat liver mitochondria and microsomes after treatment with phospholipase c. 19734202546
differences in susceptibility to phospholipase c of free and membrane-bound phospholipids of mycoplasma hominis. 19734202552
analytical determination of the buoyant density of dna in acrylamide gels after preparative cscl gradient centrifugation. 19734202826
kinetics of beta-lactamase inactivation of penicillins. ii. effect of competitive inhibitors. 19734203115
scanning electron microscopy of bacterial colonies.a technique is described for observing bacterial colony growth. bacillus cereus, b. subtilis, and b. cereus var. mycoides were grown on strips of dialysis membrane layered on nutrient agar. microcolonies of the organisms on strips were fixed in formalin vapor in situ; the strips then were removed from the agar and secured to scanning microscope specimen stubs without markedly disturbing the cellular arrangement. scanning electron micrographs clearly depict morphology of individual cells, as well ...19734203336
[effect of water activity on the development of microorganisms in fine-pored systems (kaolinite)]. 19734203670
thin layer chromatography methods for detecting hippurate hydrolase activity among various bacteria (pseudomonas, bacillus, enterobacteriaceae). 19734203919
comparative immunological and electrophoretic studies on ribosomal proteins of bacillaceae. 19734203929
comparison of ribosomal rna-binding protein "l2" isolated from different bacterial species. 19734203930
synthesis and antimicrobial properties of 17beta-amino-4-aza-5alpha-androstane and derivatives. 19744204343
unsaturated fatty acid synthesis is not required for induction of lactose transport in escherichia coli. 19744204551
[live bac. cereus in some food products]. 19734204644
biosynthesis of the crystal protein of bacillus thuringiensis var. tolworth. 2. on the relation of transcriptional and translational events in the growth cycle. 19734204719
time course of purine nucleoside phosphorylase occurrence in sporulation of bacillus cereus.purine nucleoside phosphorylase (ec 2.4.2.1) from bacillus cereus t was examined at hourly intervals during growth and sporulation. the enzyme has maximal activity in extracts prepared from cells during stages i and ii. the activity during exponential growth is only 6.6% of the maximum and that in free spores is only 3.3%. conservation of the purine nucleoside phosphorylase during sporulation is apparent as shown by the gradual increase in heat resistance.19724204910
isotopic study of control of the lysine biosynthetic pathway during sporulation of bacillus cereus.the extent of incorporation of aspartate into dipicolinic acid and into various amino compounds was determined in bacillus cereus at various times before, during, and near the end of synthesis of dipicolinic acid. the purpose of this study was to gain further information on the in vivo control of the biosynthesis of amino acids derived from aspartate. control of the lysine biosynthetic pathway was of particular interest with regard to sporulation, owing to the important role of diaminopimelate a ...19724204913
[fixation with tapo in ultrastructural study of bacterial cells. i. cell wall of bacillus cereus in ultra-thin sections]. 19724204943
[chemical and physico-chemical properties of the exo-enterotoxin of bacillus cereus]. 19734204956
association of bacillus cereus with food poisoning in dogs. 19744205050
chemical composition and ultrastructure of native and reaggregated membranes from protoplasts of bacillus cereus.conditions were defined for producing protoplasts with lysozyme and isolating the protoplast membranes from cells of bacillus cereus t harvested late in the exponential growth phase just before sporogenesis. the membranes contained approximately 60% protein, 30% lipid, 6% carbohydrate, and 1% ribonucleic acid. seventeen proteins were distinguished by molecular size in the membrane solubilized with sodium dodecyl sulfate, and 12 in that with phenol and acetic acid. the lipid fraction consisted of ...19744205199
[asimple apparatus for measuring the efficiency of quick acting disinfectants and sterilizing solutions. application to the action of sodium hypochlorite against bacterial spores]. 19734205286
[bacterial food poisoning caused by bac. cereus]. 19734205334
[the variability of actinomyces aureoverticillus 1306]. 19734205356
improved procedures for the determination of oxytetracycline in milk, milk products; chicken muscle, liver; and eggs. 19724205446
letter: bacillus cereus endocarditis in an addict. 19744205842
characterisation & identification of two aerobic spore forming bacteria producing extracellular glutamic acid & valine respectively. 19734205940
a cryptic prophage carried by bacillus cereus. 19734205982
the relative susceptibility of four cephalosporins to a beta-lactamase from bacillus cereus.the effects of purified beta-lactamase from bacillus cereus on the activity of four cephalosporins are described. drug inactivation was measured spectrophotometrically and confirmed by microbiological assay and by microbiological techniques involving determinations of minimum inhibitory concentrations and growth curves of staphylococcus aureus nctc 6571 (oxford) in broth containing drug +/- enzyme. cephaloridine was the most sensitive of the four cephalosporins, followed by cephalothin and the s ...19734206088
[submicroscopic structure of the bacterial cell wall (review of the literature)]. 19734206610
[bacillus cereus as the cause of bovine mastitis. i. report on the occurrence of b-cereus mastitis and on studies concerning infection of sheep and cows with the isolated strain]. 19734206759
antimicrobial activity of sodium copper chlorophyllin. 19744206818
use of ultraviolet radiation to locate dipicolinic acid in bacillus cereus spores.a novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (dpa) in spores. ultraviolet (uv) irradiation is shown to cause cross-linkage of dpa to spore proteins which have the characteristics of membrane proteins. we suggest that dpa resides in the inner forespore membrane (ifsm) and spore cytoplasm (i.e., the spore protoplast). only that portion of the dpa in the vicinity of the ifsm appeared to form uv-induced dpa-protein adducts.19744206870
phospholipid metabolism and membrane synthesis during sporulation in bacillus megaterium.in view of previously published reports of localized membrane growth in exponentially growing bacillus megaterium and in sporulating bacillus cereus, an attempt was made to describe phospholipid metabolism and the topology of membrane synthesis during sporulation in b. megaterium. the cells were pulsed with radioactive glycerol or acetate at the time of septum formation, and the specific activity of the lipid fraction was measured at various times through the free spore stage. the bulk of the ma ...19744206872
[enterotoxins produced by staphylococci and bacillus cereus]. 19734206898
early biochemical events occurring after infection of bacillus cereus 569-sp1 with bacteriophage gsw.after infection of bacillus cereus 569-sp1 with the 5-hydroxymethyluracil-containing phage gsw, new dttpase, dutpase, and dump-hydroxymethylase activities appear. no significant changes in activities of other pyrimidine ribonucleoside or 2'-deoxyribonucleoside triphosphate nucleotidohydrolases were detected. dutp and dump inhibit the dttpase activity, whereas dttp failed to inhibit dutpase activity. the k(m) value for the substrate dutp is 10(-4) m and for dttp is 4.85 x 10(-4) m. thymidylate sy ...19744206941
effects of concentration of volatile metabolites from bacteria and germinating seeds on fungi in the presence of selective absorbents. 19744207172
on earlier states of the biochemical system. 19744207200
[bovine mastitis due to bacillus cereus. ii. antibiogram and pathogenicity tests of the isolated strain]. 19744207432
[the concentration of inclusion endotoxin in cultures of entomopathogenic crystal forming bacilli]. 19734207538
two outbreaks of bacillus cereus food poisoning in canada. 19744207768
mesosomes in bacillus cereus 569 and the production of extra membranes by treatment with actinomycin-d. 19744207871
simple photometric assay of beta-lactamase activity.a simple photometric assay of beta-lactamase activity was developed. the method is based on a decrease in optical density at 620 nm caused by the formation of a penicilloic acid-iodine complex. the enzymatic reaction is instantaneously stopped by the addition of a concentrated iodine-tungstate solution. data showing the time and concentration dependence of the reaction are presented. by varying both the time of the assay and the concentration of the enzyme, substrates of widely different v(max) ...19724207957
effect of oxygen on heme and cytochrome content in some facultative bacteria. 19734208579
a method of freeze-drying and fixing bacterial cultures for electron microscopy. 19734208668
[electrokinetic properties of the cells of microorganisms and their taxonomy]. 19734208671
[methods of working with bacterial microcultures]. 19734208673
[newly discovered sugars in the specific polysaccharides of bacteria]. 19724208820
novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.a new cephalosporin with a highly reactive beta-lactam ring was found to give an immediate color change in the presence of beta-lactamases from many bacteria, including staphylococci, bacillus species, enterobacteriaceae, and pseudomonas. the reaction is confined to organisms producing beta-lactamases, but it is sufficiently sensitive to indicate the presence of this enzyme is small amounts in strains previously considered not to produce it. the compound has an unusual ultraviolet spectrum, and ...19724208895
effect of glucose on the biosynthesis of the membranes of bacillus. 19744209038
[experiments and considerations on the sterilization of thermolabile materials with peracetic acid]. 19744209084
control of cell division in bacteria. 19744209200
evidence for membrane cholesterol as the common binding site for cereolysin, streptolysin o and saponin. 19744209248
letter: four minor antibiotics from macarbomycins. 19734209530
microcalorimetry applied to certain species of bacteria growing in sterilized separated milk. 19744209942
contamination of blood cultures from a commercial beta-lactamase preparations. 19744210011
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