| hygienic hand disinfection for the removal of epidemic vancomycin-resistant enterococcus faecium and gentamicin-resistant enterobacter cloacae. | an outbreak strain of enterococcus faecium, bearing plasmid-mediated vancomycin resistance, and an epidemic gentamicin-resistant, multiply-resistant strain of enterobacter cloacae, both survived well on fingertips of three volunteers for up to 30 minutes after inoculation. hand disinfection of inoculated fingers with 60% isopropyl alcohol, or with alcoholic chlorhexidine, reliably gave a 4 log10 reduction of both test organisms. enterobacter cloacae could not be detected in any finger washings, ... | 1991 | 1680903 |
| enterococcus faecium sensitive to teicoplanin but not vancomycin. | | 1991 | 1682372 |
| the fluoroquinolones exert a reduced rate of kill against enterococcus faecalis. | the bactericidal activities of ciprofloxacin, ofloxacin and dr-3355 have been investigated against enterococcus faecalis, staphylococcus aureus and staphylococcus epidermidis over 24 h. the three fluoroquinolones were found to be rapidly bactericidal against the staphylococci, killing over 99% of the bacteria during the first 3 h of exposure with a further reduction in viability of approximately one logarithm occurring over the next 21 h. in contrast, the fluoroquinolones displayed a much slower ... | 1991 | 1682462 |
| evidence for the genetic unrelatedness of nosocomial vancomycin-resistant enterococcus faecium strains in a pediatric hospital. | vancomycin-resistant enterococci are now found with increasing frequency. up to now, epidemiological studies of enterococci have been limited by the lack of convenient and accessible methods for comparing strains. in this study, we report an epidemiological investigation on 16 nosocomial vancomycin-resistant enterococcus faecium strains isolated from 15 patients in four different wards of a children's hospital over a period of 17 months. analysis of restriction fragment length polymorphism (rflp ... | 1991 | 1685496 |
| the killing activity of microwaves on some non-sporogenic and sporogenic medically important bacterial strains. | the killing activity of microwaves of 2450 mhz frequency and 325 w, 650 w and 1400 w power on some bacterial strains was investigated. vegetative strains of staphylococcus aureus, streptococcus pyogenes group a, escherichia coli, pseudomonas aeruginosa and enterococcus faecalis and spores of bacillus subtilis and bacillis stearothermophilus in aqueous suspensions were exposed to 325 w and 650 w waves for different lengths of time. enterococcus faecalis and spores of b. subtilis and b. stearother ... | 1991 | 1686036 |
| 16s rrna sequence determination for members of the genus carnobacterium and related lactic acid bacteria and description of vagococcus salmoninarum sp. nov. | the phylogenetic interrelationships of members of the genus carnobacterium and some atypical lactobacilli isolated from diseased salmonid fish were investigated by using reverse transcriptase sequencing of 16s rrna. the four species carnobacterium piscicola, carnobacterium divergens, carnobacterium gallinarum, and carnobacterium mobile exhibited a high degree of sequence similarity with each other (ca. 96 to 98%) and formed a phylogenetically coherent group that was quite distinct from all other ... | 1990 | 1697764 |
| antibacterial activity of phenethyl alcohol and resulting membrane alterations. | the antibacterial activity of phenethyl alcohol (pea) towards gram-negative (escherichia coli and pseudomonas aeruginosa) and gram-positive bacteria (staphylococcus aureus and enterococcus faecium) was investigated. this activity was expressed as ic (inhibitory concentration) and bc (bactericidal concentration). pea was bactericidal in the concentration range of 90 to 180 mm, these concentrations being 4- to 5-fold higher than the corresponding ic. the mechanism of action of pea upon the cell me ... | 1990 | 1697975 |
| [the detection of streptococcal cell-wall proteins that form complexes with human macroglobulins]. | the composition of the extracts of the cultures of individual streptococcal strains, studied by immunoblotting techniques, has been shown to contain proteins with a molecular weight of 70-80 kd. these proteins have pronounced affinity to human macroglobulins: alpha-macroglobulin, alpha-glycoprotein associated with pregnancy and protein a. the significance of this phenomenon on the cellular and somatic levels is discussed. | 1990 | 1700566 |
| induction of autolysis in enterococcus faecalis s-47 by peptide as-48. | in addition to its bactericidal mode of action, the peptide antibiotic as-48 exhibits a bacteriolytic effect on enterococcus faecalis s-47 that is associated with autolysin activation. bacteriolysis induced by the antibiotic can be modulated by addition of edta, divalent cations and autolysin activators (trypsin) or inhibitors (cardiolipin), suggesting that topologic regulation of the autolysins is involved in the process. in addition, inhibitors of protein and rna synthesis interfere markedly w ... | 1990 | 1700974 |
| permeation of bacterial cells, permeation of cytoplasmic and artificial membrane vesicles, and channel formation on lipid bilayers by peptide antibiotic as-48. | peptide as-48 induces ion permeation, which is accompanied by the collapse of the cytoplasmic membrane potential, in sensitive bacteria. active transport by cytoplasmic membrane vesicles is also impaired by as-48. at low concentrations, this peptide also causes permeability of liposomes to low-molecular-weight compounds without a requirement for a membrane potential. higher antibiotic concentrations induce severe disorganization, which is visualized under electron microscopy as aggregation and f ... | 1991 | 1702784 |
| phylogenetic analysis of streptococcus saccharolyticus based on 16s rrna sequencing. | reverse transcriptase sequencing of 16s rrna of streptococcus saccharolyticus was performed in order to determine the phylogenetic position of this organism. on the basis of the sequence data streptococcus saccharolyticus formed a distinct group with enterococcus faecalis (type species of the genus enterococcus) and other enterococcal species. streptococcus saccharolyticus was found to be only distantly related to members of the genus streptococcus sensu stricto. it is therefore proposed that st ... | 1990 | 1703505 |
| use of rapid, nonradioactive dna probes in culture confirmation tests to detect streptococcus agalactiae, haemophilus influenzae, and enterococcus spp. from pediatric patients with significant infections. | acridinium ester-labeled, chemiluminescent dna probe tests (accuprobe; gen-probe, inc.) for culture confirmation of streptococcus agalactiae, haemophilus influenzae, and enterococcus spp. were compared with conventional identification techniques. the probe is a dna oligomer that is a complementary to the rna of the target. the dna-rna hybrids are measured in a luminometer. the 40-min assay requires one reaction tube and the addition of three reagents. when two colonies were used to add a sample ... | 1991 | 1704385 |
| intrageneric relationships of enterococci as determined by reverse transcriptase sequencing of small-subunit rrna. | the 16s ribosomal ribonucleic acid (rrna) sequences of eleven enterococcus species were determined by reverse transcription in an attempt to clarify their intrageneric relationships. comparative analysis of the sequence data revealed the presence of several species groups within the genus. the species e. avium, e. malodoratus, e. pseudoavium and e. raffinosus formed a distinct group as did e. durans, e. faecium, e. hirae and e. mundtii and the pair of species e. casseliflavus and e. gallinarum. ... | 1991 | 1712504 |
| relationship of the chemical structure and immunobiological activities of lipoteichoic acid from streptococcus faecalis (enterococcus hirae) atcc 9790. | two molecular species of lipoteichoic acid (lta 1 and lta 2) were isolated from whole cells of streptococcus faecalis (enterococcus hirae) atcc 9790 by hydrophobic chromatography on octyl sepharose cl-4b. chemical analysis revealed that lta 1 and lta 2 contained two and four acyl lipid anchors respectively. lta 1 was less active than lta 2 in inducing cytokines, except interleukin-1 (il-1), but their in vivo antitumour effects were similar. lta 2 was a potent inducer of tumour necrosis factor (t ... | 1991 | 1718341 |
| cloning, sequence and overexpression of nadh peroxidase from streptococcus faecalis 10c1. structural relationship with the flavoprotein disulfide reductases. | dna fragments encoding streptococcal nadh peroxidase (npxase) have been amplified, cloned and sequenced from the genome of streptococcus (enterococcus) faecalis 10c1 (atcc 11700). the npxase gene (npr) comprises 1341 base-pairs and is preceded by a typical ribosome binding site. upstream from the structural gene, putative -10 and -35 promoter regions have been identified, as has a possible factor-independent terminator that occurs in 3'-flanking sequences. the deduced relative molecular mass (mr ... | 1991 | 1719212 |
| use of low molecular mass rna profiles to identify lactic acid bacteria and related organisms associated with foods. | fourteen strains of lactic acid bacteria and species of brochothrix, carnobacterium, enterococcus, erysipelothrix, kurthia and listeria were examined using low molecular mass rna (5s rrna and trna) profiles. these profiles were developed on denaturing polyacrylamide gels. gel strengths between 9 and 14% were tested to improve resolution of distinct bands for densitometrical analysis. profiles generated on 12% gels proved to be the best for scanning. scans of class 2 trnas by densitometry showed ... | 1991 | 1723291 |
| [kinetics of bacterial wash-out in the haemonetics cell saver iii]. | autologous, outdated blood units were inoculated with four types of bacteria--pseudomonas aeruginosa, escherichia coli, streptococcus faecalis, staphylococcus aureus--in two concentrations (10e4, 10e6) to test the effect of the washing procedure (cell saver iii haemonetics) on the elimination of bacteria. the elimination rate ranged from 17% (streptococcus faec. 10e4) to 96% (pseudomonas aer. 10e6). these results confirm the routine use of wash centrifuge systems (type cell saver) to process she ... | 1991 | 1725651 |
| addition of cetyl pyridinium chloride to histological demineralizing agents to improve the demonstrability of gram-positive bacteria. | it has previously been shown experimentally using s. faecalis that the relative number of gram-positive bacteria is reduced by demineralizing agents. to achieve better bacterial preservation after demineralization, cetyl pyridinium chloride (cpc) and neutral formaldehyde solution (nf) were added, separately and in combination, to formic acid and edta. the number of s. faecalis organisms in suspension was determined using a haemocytometer, and the percentage of gram-positive bacteria was ascertai ... | 1991 | 1725981 |
| the cumulative effect of disinfection, storage, histological fixation and demineralization on number and staining ability of gram-positive bacteria. | it has previously been shown experimentally using s. faecalis that the relative number of blue-staining gram-positive bacteria was reduced by demineralizing agents as well as by disinfection (wijnbergen & van mullem 1987, van mullem & wijnbergen 1989). in this study the cumulative effect of disinfection, experimental period, fixation and demineralization was investigated. the use of additives to the fixative and the demineralizing agents to limit the loss of blue-staining bacteria was also studi ... | 1991 | 1725982 |
| randomized trial of meatal care with silver sulfadiazine cream for the prevention of catheter-associated bacteriuria. | a randomized, controlled, prospective clinical trial involving 696 hospitalized patients was undertaken to determine the effectiveness of 1% silver sulfadiazine cream applied twice daily to the urethral meatus in preventing transurethral catheter-associated bacteriuria. the overall incidence of bacteriuria was 11.4% (38/332) in the treated group and 13.2% (48/364) in the untreated group (p = .56; odds ratio, 0.85; 95% confidence interval, 0.53-1.37). cox proportional hazards analysis identified ... | 1992 | 1727882 |
| aggregation substance of enterococcus faecalis mediates adhesion to cultured renal tubular cells. | the sex pheromone system of enterococcus faecalis is a unique, highly efficient plasmid collection mechanism for this species. a crucial role in this system is played by an adhesin called aggregation substance which enables the cell-cell contact between donor and recipient strains. the existence of the amino acid motif arg-gly-asp-ser in the adhesin prompted us to look for a possible binding of e. faecalis cells expressing aggregation substance to eucaryotic cells. we were able to show that the ... | 1992 | 1729187 |
| isolation and characterisation of the pyruvate dehydrogenase complex of anaerobically grown enterococcus faecalis nctc 775. | in this contribution the isolation and some of the structural and kinetic properties of the pyruvate dehydrogenase complex (pdc) of anaerobically grown enterococcus faecalis are described. the complex closely resembles the pdc of other gram-positive bacteria and eukaryotes. it consists of four polypeptide chains with apparent molecular masses on sds/page of 97, 55, 42 and 36 kda, and these polypeptides could be assigned to dihydrolipoyl transacetylase (e2), lipoamide dehydrogenase (e3) and the t ... | 1992 | 1730230 |
| ["spontaneous" bacterial peritonitis]. | | 1992 | 1732842 |
| infections due to beta-lactamase-producing, high-level gentamicin-resistant enterococcus faecalis. | to investigate the risk factors, clinical features, molecular epidemiology, and treatment outcomes associated with an outbreak of infections due to beta-lactamase-producing, high-level gentamicin-resistant enterococcus faecalis. | 1992 | 1733382 |
| bacteriology of anal fistulae. | anal fistulae are said to arise from cryptoglandular infection of the anal glands, which lie within the intersphincteric space. the type and virulence of the micro-organism responsible may determine whether an anal fistula develops. the microbiology of chronic anal fistulae has not been reported previously. twenty-five consecutive anal fistulae were studied prospectively (eight intersphincteric fistulae, 12 trans-sphincteric fistulae, two suprasphincteric fistulae, one extrasphincteric fistula, ... | 1992 | 1737267 |
| enterococcus faecium strains susceptible to ampicillin. | | 1992 | 1737727 |
| the relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women. | lactobacilli provide an important microbial defense against genital colonization by pathogens. the role of hydrogen peroxide (h2o2) in the control of genital microflora was explored in a cross-sectional study of 275 women in the second trimester of pregnancy. vaginal cultures were obtained for detection of h2o2-positive and h2o2-negative lactobacilli and other members of the genital microflora. compared with women with h2o2-negative lactobacilli, women colonized by h2o2-positive lactobacilli wer ... | 1992 | 1738516 |
| active-site structural comparison of streptococcal nadh peroxidase and nadh oxidase. reconstitution with artificial flavins. | the apoproteins of the streptococcal nadh peroxidase (h2o2----2h2o) and nadh oxidase (o2----2h2o) stabilize the neutral forms of 6-hydroxy- and 6-mercapto-fad, respectively. the redox behavior of the 6-hydroxy-fad peroxidase closely mimics that of the native enzyme with both dithionite and nadh. both oxidase and peroxidase preferentially stabilize the n(1)-protonated p-quinonoid species of 8-mercapto-fad, and the 8-position of the bound flavin is accessible to solvent in both proteins. the 8-mer ... | 1992 | 1740431 |
| bacteriological nasal flora in newborns indicating health and/or development of infection. | during delivery, a fetus otherwise sterile during the intrauterine life, comes in contact with bacterial flora of the mother's birth canal, and then also with the ward personnel's skin and respiratory system flora. due to the absence of competitive bacteriological flora, the nasal cavity is gradually colonized by microorganisms, especially those with a capacity of adhesion to epithelial cells of respiratory nasal mucosa. bacteriological flora of the newborn's nasal flora was observed on days 1 a ... | 1991 | 1743875 |
| is primary sensitivity testing on urine samples valid? | one thousand eight hundred urine specimens were examined prospectively to determine the validity of primary sensitivity testing. microscopic criteria assessing pyuria and microorganisms were developed for predicting the presence of urinary tract infection and thus suitability for direct sensitivity testing. the criteria selected gave a positive predictive value of 74.6% and a negative predictive value of 99.5%. zone diameters by primary and standardized secondary methods were compared for each u ... | 1991 | 1745566 |
| [the effect of various culture parameters on the yield of restriction endonuclease sfa ni]. | a simple technique is proposed for testing the restriction endonuclease sfa ni activity in lysates of streptococcus faecalis cells. the technique was used to study the effect of inorganic phosphate and the growth phase on the enzyme yield. conditions were chosen that provide a high yield of the sfa ni activity and a significantly reduced level of nucleases in the cells. | 1991 | 1745646 |
| enterococcus hirae infection and focal necrosis of the brain of chicks. | | 1991 | 1746106 |
| enterococcus hirae in different animal species. | | 1991 | 1746125 |
| selection and application of streptococcus bovis as a silage inoculant. | three strains of streptococcus bovis, a homolactic bacterium capable of utilizing starch, were evaluated for growth kinetics and ability to decrease the ph of alfalfa silage. a selected strain was evaluated for its competitiveness as an inoculant with enterococcus faecium, an organism used in inoculants, and for its ability to enhance the effect of a commercial inoculant. testing was completed over three studies using wilted alfalfa (28 to 34% dry matter) ensiled into laboratory silos. treatment ... | 1991 | 1746960 |
| the enterococcus hirae r40 penicillin-binding protein 5 and the methicillin-resistant staphylococcus aureus penicillin-binding protein 2' are similar. | the penicillin-resistant enterococcus hirae r40 has a typical profile of membrane-bound penicillin-binding proteins (pbps) except that the 71 kda pbp5 of low penicillin affinity represents about 50% of all the pbps present. water-soluble tryptic-digest peptides were selectively produced from pbp5, their n-terminal regions were sequenced and synthetic oligonucleotides were used as primers to generate a 476 bp dna fragment by polymerase chain reaction. on the basis of these data, the pbp5-encoding ... | 1991 | 1747121 |
| cavernous sinus thrombosis following odontogenic and cervicofacial infection. | cavernous sinus thrombosis (cst) is rarely seen clinically as a complication of infectious processes since the discovery of penicillin. at the present time, dental abscess is an uncommon cause of cst. we now report our experiences with a 60-year-old diabetic male, who developed cst 38 days after extraction of an infected upper third molar tooth. the importance of eradicating regional cervicofacial foci of infection is stressed. | 1991 | 1747254 |
| in vitro activity of the novel cephalosporin gr69153. | the in vitro activity of gr69153 was compared to that of ceftazidime, ceftriaxone, imipenem and gentamicin against a total of 702 recent clinical isolates. mics were determined by a standard agar dilution procedure and two inocula (10(4) and 10(8) cfu) were used throughout. gr69153 inhibited 90% of isolates of escherichia coli, klebsiella pneumonia and proteus mirabilis at less than or equal to 0.25 mg/l and 90% of isolates of pseudomonas aeruginosa at less than or equal to 1 mg/l. citrobacter f ... | 1991 | 1748126 |
| evaluation of the improvement of cephems on the prophylaxis of pelvic infection after radical hysterectomy. | antibiotics, especially cephems, have been improved remarkably in the last 20 years. in order to evaluate the improvement of antibiotics used for the prophylaxis of infections after radical hysterectomy, two groups of patients (group a, 1978-1979, n = 54; group b, 1985-1987, n = 55) were examined in regard to bacteriological and clinical effects. in spite of the improvement of antibiotics, the positive rate of bacteriological analysis had increased from 44.4 to 76.4%. obvious decrease of gram-ne ... | 1991 | 1748317 |
| low cost device for electrotransformation and its application to the highly efficient transformation of escherichia coli and enterococcus faecalis. | a simple, low cost device for electrotransformation has been designed and constructed. the cost of the circuit was only $150. maximum field strength of 12,000 v/cm with an actual time constant up to 11 msec was obtained with a newly designed circuitry and a 0.1-cm electrode gap cuvette. eschericia coli strains dh1, dh5 alpha, and le392 were transformed at an efficiency of 10(9)/micrograms dna with plasmids puc18 and pbr322. e. faecalis strain og1x was transformed at an efficiency of 0.9 x 10(5)/ ... | 1991 | 1749819 |
| endovascular infections caused by enterococci highly resistant to ampicillin. | enterococci frequently cause endocarditis and are the most common gram-positive isolates in polymicrobial bacteremia. we report three cases of polymicrobial endovascular infections at a single institution during a 12-month period; the enterococcal isolates were highly resistant to penicillins. these cases comprised 18% of all enterococcal endovascular isolates during the same 12-month period. previous use of antibiotics, presence of endovascular catheters, and nosocomial acquisition of the organ ... | 1991 | 1749972 |
| bacteriological quality of potable water sources supplying morogoro municipality and its outskirts: a case study in tanzania. | bacteriological quality of potable water supplying morogoro municipality and its outskirts (population 135,000 people) was assessed by the determination of the most probable number (mpn) of faecal coliforms, escherichia coli, faecal streptococci and clostridium perfringens for a period of 6 months. river water and chlorinated pipeborne water were found contaminated with microorganisms in the order of 3.8 x 10(1) to 4.95 x 10(3); 3.2 x 10(1) to 4.5 x 10(3); 10(1) to 6.4 x 10(1) and 1.2 x 10(1) to ... | 1991 | 1752297 |
| streptococcus faecalis in neonatal infective endocarditis. | | 1991 | 1753124 |
| differentiation of lactococcus lactis and lactococcus garvieae from humans by comparison of whole-cell protein patterns. | we tested 12 reference and 24 clinical strains of lactococci for physiologic characteristics using a conventional test system, the gen-probe enterococcus 2 chemiluminescence assay (gen-probe inc., san diego, calif.), the rapid strep identification system (analytab products, plainview, n.y.), and whole-cell protein analysis. the gen-probe enterococcus 2 chemiluminescence assay for enterococcus identification was negative with all strains. neither the conventional test nor the rapid strep identifi ... | 1991 | 1757541 |
| dna fingerprinting of enterococcus faecium by pulsed-field gel electrophoresis may be a useful epidemiologic tool. | pulsed-field gel electrophoresis was used to compare 34 isolates of enterococcus faecium from six different geographic locations. this procedure generated an average of 13 discernible fragment bands per isolate (range, 10 to 19 fragment bands) of 34 to 485 kb. the resulting restriction endonuclease digestion patterns were quite heterogeneous and were able to differentiate 27 of 34 isolates from each other, as defined by one or more mismatched fragment bands. five patterns were shared by two or m ... | 1991 | 1757545 |
| evaluation of the vitek gps-ta card for laboratory detection of high-level gentamicin and streptomycin resistance in enterococci. | the vitek gps-ta card (vitek systems, hazelwood, mo.) was compared with single-concentration broth microdilution and disk diffusion methods using high-content disks for the detection of high-level resistance to gentamicin and streptomycin in 99 isolates of enterococci (81 enterococcus faecalis isolates and 18 enterococcus faecium isolates). the gps-ta card accurately detected high-level resistance to gentamicin, but not streptomycin, in e. faecalis. when streptomycin is being considered for ther ... | 1991 | 1757562 |
| characterization of yellow-pigmented enterococci from severe human infections. | four strains of yellow-pigmented enterococci that resembled the species enterococcus casseliflavus were isolated from patients who had undergone surgical treatment. they were substantially homologous in terms of biochemical properties, antibiotic susceptibilities, and plasmid dna profiles. yellow-pigmented enterococci could be another potentially important cause of nosocomial infection in surgical units. | 1991 | 1757566 |
| [vancomycin in 1991: current status and perspectives]. | vancomycin is a narrow-spectrum glycopeptide antibiotic which is primarily active against gram-positive organisms. bacterial resistance develops rarely due to its numerous modes of action. the mode of action of vancomycin involves the inhibition of peptidoglycan synthesis. vancomycin forms a stoichiometric complex with the peptidoglycan precursor udp-n-acetylmuramyl pentapeptide by forming hydrogen bonds. in patients with renal insufficiency vancomycin clearance is reduced and elimination half-l ... | 1991 | 1758723 |
| [the characteristics of staphylococcus aureus peptidoglycan in the spectrum of the reactivity of human lymphocytes to bacterial peptidoglycans]. | the sensitivity of lymphocytes of healthy persons to s. aureus peptidoglycan as compared with that to the polyclonal stimulator zymosan c3b and peptidoglycans of other bacteria (streptococcus faecalis, escherichia coli, bacterium bifidum) was analyzed with a test system permitting the determination of specific reactivity to peptidoglycans. the analysis showed that at the peak of luminol-dependent chemiluminescence (25-30 minutes) individual reactivity to s. aureus peptidoglycan varied within wid ... | 1991 | 1759526 |
| [clinical features of enterococcal septicemia and antimicrobial susceptibilities for clinical isolates of enterococci in nagoya university hospital]. | enterococci are important causative organisms of nosocomial infections as well as methicillin resistant staphylococcus aureus and pseudomonas aeruginosa. enterococcal bacteremia has been increased in nagoya university hospital from april 1983 to march 1990. we analyzed the clinical backgrounds, portal of entry and mortality for the patients with enterococcal septicemia. thirty-four cases with enterococcal septicemia (24 enterococcus faecalis, 10 enterococcus faecium) were subjected in this study ... | 1991 | 1761891 |
| [gram-positive cocci in the urinary tract. do they influence the postoperative course of transurethral prostatectomy?]. | the influence of gram-positive cocci on the postoperative course of transurethral prostatectomy was analysed on 463 patients. the half of isolated cocci was enterococcus, followed by s. epidermidis and s. aureus. the rates of postoperative urinary infection by gram-negative bacilli were nearly the same for patients with preoperative gram-positive cocci infection and those without any of bacteria (3/79 vs. 9/310). patients who developed new infection with gram-negative bacilli had fever, in contr ... | 1991 | 1762271 |
| small and medium-angle x-ray analysis of bacterial lipoteichoic acid phase structure. | x-ray scattering analysis was performed on various types of bacterial lipoteichoic acid in solution. the x-ray data show that all samples investigated were characterized by a similar micellar ultrastructure (hydrophilic moiety on the outside) with a fatty acid chain conformation of the disordered, alpha-type at all temperatures between 5 degrees-53 degrees c. the size distribution of staphylococcus aureus lipoteichoic acid micelles was sufficiently homogeneous to determine their size and some re ... | 1991 | 1765082 |
| detection of penicillin-binding proteins immunologically related to penicillin-binding protein 5 of enterococcus hirae atcc 9790 in enterococcus faecium and enterococcus faecalis. | penicillin-binding protein (pbp) 5 of enterococcus hirae atcc 9790 belongs to the class of the high-molecular mass, low-affinity pbps which have been correlated with penicillin resistance in most enterococcus species. polyclonal antibodies were raised against pbp 5 and used to detect immunologically related membrane proteins in e. faecium and e. faecalis strains. several strains of both species were found to have a membrane protein of similar molecular mass to e. hirae pbp 5 which reacted with t ... | 1991 | 1769542 |
| the garrod lecture. the enterococcus: a classic example of the impact of antimicrobial resistance on therapeutic options. | | 1991 | 1769929 |
| rapid isolation and presumptive diagnosis of uropathogens by using membrane filtration and differential media. | random urine samples from hospitalized patients (n = 550) and seeded sterile filtered urine samples (n = 730) were used to test a membrane filtration technique, qualture (future medical technologies international, inc., west palm beach, fla.), for the detection and identification of uropathogens. results for each sample were compared with those obtained by the calibrated loop (0.01 ml) method to demonstrate the sensitivity of the method as a screening tool and the specificity of the presumptive ... | 1991 | 1774240 |
| recovery of vancomycin-resistant gram-positive cocci from pediatric liver transplant recipients. | between november 1988 and october 1989, 49 first-time pediatric liver transplant recipients at the children's hospital of pittsburgh were prospectively monitored for the presence of stool colonization and the development of disease caused by vancomycin-resistant gram-positive cocci (vrgpc). quantitative stool culturing was done on a weekly basis, and cultures were planted onto a selective medium for vrgpc. isolates for which the mic was greater than or equal to 8 were considered resistant to van ... | 1991 | 1774255 |
| epidemiology of an endemic strain of beta-lactamase-producing enterococcus faecalis. | three previously reported beta-lactamase-producing (bla+) enterococci with distinct but related antibiotic resistance phenotypes, plasmid profiles, and plasmid restriction endonuclease digestion patterns were isolated at the west haven veterans administration medical center in connecticut (wh245, wh257, wh571) in july 1986 and march 1987. in this study, we analyzed the whole-cell dna of these isolates by using pulsed-field gel electrophoresis of large chromosomal fragments generated by smai dige ... | 1991 | 1774257 |
| detection of high-level aminoglycoside resistance in enterococci other than enterococcus faecalis. | the ability of six screening methods to detect high-level aminoglycoside resistance in enterococcal species other than enterococcus faecalis was investigated. the 85 enterococcus isolates, which included 55 e. faecium, 11 e. gallinarum, 9 e. casseliflavus, 5 e. raffinosus, 4 e. avium, and 1 e. mundtii, were tested by using aminoglycoside-supplemented brain heart infusion agar (bhi), remel ef synergy quad plates, high-content aminoglycoside diffusion disks, standard (prepared in-house) microdilut ... | 1991 | 1774267 |
| inability of cephalothin testing to predict cefprozil susceptibility. | the 30-micrograms cefprozil disk and the cephalosporin class (30-micrograms cephalothin) disk were compared for their abilities to predict cefprozil susceptibility by agar disk diffusion testing. high error (5.02% major and 14.11% minor) rates were encountered with the cephalothin disk and were most frequently observed for escherichia coli and enterococcus faecalis. the cefprozil disks resulted in rates of 0.05% very major, 0% major, and 3.72% minor errors. thus, the 30-micrograms cefprozil disk ... | 1991 | 1774280 |
| ampicillin-resistant enterococcus raffinosus in an acute-care hospital: case-control study and antimicrobial susceptibilities. | a prospective study identified 9 (32%) of 28 ampicillin-resistant (mic greater than or equal to 16 micrograms/ml) enterococcus isolates as enterococcus raffinosus. a case-control study found no significant differences with respect to underlying diseases, catheterization, or surgery between patients with ampicillin-resistant e. raffinosus and those with ampicillin-susceptible enterococcus spp. prior treatment with antibiotics and prolonged hospitalization were more frequent among patients with am ... | 1991 | 1774284 |
| factors influencing determination of high-level aminoglycoside resistance in enterococcus faecalis. | the ability of seven methods to detect high-level gentamicin (58 strains) and streptomycin resistance (56 strains) among 107 enterococcus faecalis isolates was investigated at the university of chicago medical center and the university of nebraska medical center. methods included a standard agar screen plate, high-content disk diffusion, remel (lenexa, kans.) ef synergy quad plates, standard microdilution panels prepared in house, pasco mic gram-positive panels (difco laboratories, detroit, mich ... | 1991 | 1774318 |
| platelet aggregation by strains of enterococci. | the platelet aggregation capability of whole cells of enterococcus faecalis, e. faecium and e. avium was tested. the optimum ratios of bacteria to platelets in e. faecalis (strain smu-37), e. faecium (strain smu-138) and e. avium (strain smu-197) were 1.0, 1.2 and 2.0, respectively. during the platelet aggregation induced by the three strains of enterococci, 65-69% of total serotonin was released. the aggregation was totally inhibited by ethylenediaminetetraacetate (10 mm) and apyrase (1 mg/ml), ... | 1991 | 1775097 |
| prognosis of enterococcal endocarditis. | | 1991 | 1775863 |
| enterococci highly resistant to penicillin: characterizing isolates from singapore hospitals. | enterococci are increasing in importance as nosocomial pathogens and causes of severe sepsis in immunocompromised patients. from september to november 1989, a survey of 898 enterococcal isolates showed that 52 had acquired high-level resistance to penicillin and ampicillin (mic greater than 100 mg/l). these were all enterococcus faecium, did not produce beta-lactamase and showed high-level resistance to gentamicin and streptomycin as well. the majority were urinary isolates, but a few caused bac ... | 1991 | 1776005 |
| purification, characterization, and biological effects of a second bacteriocin from enterococcus faecalis ssp. liquefaciens s-48 and its mutant strain b-48-28. | enterococcus faecalis ssp. liquefaciens s-48 (producer of the peptide antibiotic as-48) and its mutant b-48-28 (as-48-) secrete the bacteriocin bc-48. this substance has been purified to homogeneity from culture supernatants of strain b-48-28; it consists of a protein (80 kda) stable from ph. 5.5 to 9.0 and sensitive to temperatures above 45 degrees c and to proteases. its inhibitory spectrum is restricted to strains of enterococcus faecalis. bc-48 inhibits protein synthesis but does not affect ... | 1991 | 1777854 |
| enterococcus faecalis: specific and non-specific interactions with human polymorphonuclear leukocytes. | in previous studies we have demonstrated that the ability of enterococcus faecalis to adhere to and to be internalized in human urinary tract epithelial cells, girardi heart cells and human polymorphonuclear leukocytes (pmns), was dependent on whether the strain had been isolated from urinary tract infections (uti) or endocarditis (en) respectively. these properties were further modified by growth of the organism in human serum. in the present report, using competition assays we show that adhesi ... | 1991 | 1778438 |
| factors influencing the synthesis of an extracellular proteinase by enterococcus faecalis subsp. liquefaciens. | studies were conducted on optimum temperature, ph and the requirement for an energy source, amino acids, casein, zn2+ and ca2+ during the synthesis of an extracellular acid proteinase by enterococcus faecalis var. liquefaciens. synthesis was monitored using cells grown to mid-logarithmic phase and resuspended at high density in fresh growth medium. proteinase production was optimal at 30 degrees c and ph 7.0. proteinase synthesis, being energy-dependent, occurred only in glycolysing cells. the s ... | 1991 | 1780008 |
| interpretive criteria of antimicrobial disk susceptibility tests with flomoxef. | 320 recently isolated pathogens, 20 strains from each of 16 species, were investigated using mueller-hinton agar and din as well as nccls standards. the geometric mean of the agar dilution mics of flomoxef were 0.44 mg/l for staphylococcus aureus, 0.05 mg/l (klebsiella oxytoca) to 12.6 mg/l (enterobacter spp.) for enterobacteriaceae, 33.1 mg/l for acinetobacter anitratus, 64 mg/l for enterococcus faecalis, and more than 256 mg/l for pseudomonas aeruginosa. for disk susceptibility testing of flom ... | 1991 | 1783442 |
| in vitro activity of flomoxef and cefazolin in combination with vancomycin. | 207 clinical isolates from strains of patients from the university children's hospital of kiel were investigated for their in vitro activity with the agar dilution method against flomoxef and cefazolin (alone and partially in combination with vancomycin). staphylococci were also tested with other cephalosporins (cefoxitin, cefamandole, cefotaxime, cefotetan and latamoxef). flomoxef and cefazolin always acted more vigorously on staphylococci than the other cephalosporins. resistance of staphyloco ... | 1991 | 1783443 |
| the in vitro activity of flomoxef compared to four other cephalosporins and imipenem. | the antibacterial activity of the oxacephalosporin flomoxef was evaluated in comparison to cefpirome, cefuzoname, cefotaxime, ceftazidime, and imipenem against fresh clinical isolates. flomoxef is an antibiotic with strong antibacterial activity against staphylococci including methicillin-resistant strains and streptococci with the exception of enterococcus faecalis and enterococcus faecium. it is very active against gram-negative cocci and rods including gram-positive and gram-negative anaerobe ... | 1991 | 1783444 |
| [bacterial meningitis in the elderly with neurosurgical procedures]. | bacterial meningitis is one of complications in the elderly with neurosurgical procedures. in an attempt to find the clinical features of this complication we analyzed 10 cases, which were found in 30 cases of the bacterial meningitis in tokyo metropolitan geriatric hospital from 1972 to 1989. the patients were 4 males and 6 females, 52-86 years old (the mean, 69). while 2 enterococcus species were isolated after craniotomy, staphylococci were common pathogens (4 s. aureus, 4 s. epidermidis and ... | 1991 | 1783805 |
| [bacteriological studies on pharyngeal and tracheal colonization in patients of operations and patients in icu]. | bacterial colonization of the pharynx before and one week after operation, and contamination of the trachea during intubation in 7 patients who underwent operations were investigated. and bacterial colonization of pharynx, tracheas, and mechanical-ventilators in 7 icu patients was investigated. flora of palms of 10 icu personnel was also examined: 1) oral bacteria began to be found in the trachea several hours after intubation. 2) in oropharyngeal flora, one week after operation, neisseria and a ... | 1991 | 1783808 |
| application of the polymerase chain reaction to study the m protein(-like) gene family in beta-hemolytic streptococci. | evaluation of homologous regions of published m protein (emm) gene sequences from group a streptococci (gas; streptococcus pyogenes) was used to design three primer pairs for polymerase chain reaction (pcr) and three oligonucleotide probe sequences internal to the amplified products. one set of primers and corresponding probe should detect and lead to amplification of emm(-like) genes of virtually every type ("all m"), another ("sor-m") should only amplify emm(-like) genes from gas negative for ... | 1991 | 1784271 |
| [bacteriological quality of groundwaters in cemeteries]. | groundwater samples collected by piezometers from three cemeteries in geologically distinct areas of s. paulo and santos, brazil, were analysed in order to determine their hygienic and sanitary conditions. fecal coliformes, fecal streptococci, sulfite reducer clostridia and salmonella were searched for the purpose of evaluating sanitary conditions, and total coliforms, heterotrophic bacteria, proteolitic and lipolitic microorganisms for evaluating hygienic conditions. in some samples, nitrate le ... | 1991 | 1784962 |
| [vulvovaginitis in premenarche girls. a preliminary study]. | the study population consisted of 832 premenarcheal girls. vaginal cultures are performed on 40 premenarcheal girls suffering from vulvovaginitis. all were less than 7 years old. this patients were seen in primary cares. in 23 cases (57.5%) e. coli was isolated, enterococcus (30%), mixed flora (10%) and g. vaginalis (2.5%). common clinical characteristics were pruritus (97.5%), vaginal discharge (67.5%). dysuria and abdominal pain constituted accompanying symptoms. only in one case masturbation ... | 1991 | 1785745 |
| the bacteriology of cholelithiasis--china versus japan. | cholelithiasis differs considerably from area to area in the world. calcium bilirubinate stones or brown pigment stones are said to predominate in the orient, however, this situation may differ within the orient. in order to compare cases in china and japan, 102 consecutive cases of cholelithiasis operated on in shenyang, china were analyzed for the composition of gallstones and bacterial species isolated from bile in relation to the location and composition of gallstones. in shenyang, calcium b ... | 1991 | 1787607 |
| enterococci highly resistant to penicillin: characterizing isolates from singapore hospitals. | | 1991 | 1788591 |
| enterococcal endocarditis causing heart failure in broilers. | | 1991 | 1788921 |
| growth inhibition of listeria spp. on camembert cheese by bacteria producing inhibitory substances. | bacterial strains exhibiting antimicrobial activity towards other bacteria are quite common in nature. during the past few years several genera have been shown to exert inhibitory action against listeria. spp. in the present work strains of enterococcus, lactobacillus and lactococcus were tested for their influence on the development of listeria spp. on camembert cheese. partial or complete inhibition of growth of listeria spp. was observed using various inhibitory bacteria. complete inhibition ... | 1991 | 1790105 |
| in vitro activity of the new glycopeptide ly264826 versus vancomycin against clinical isolates of enterococci. | | 1991 | 1794373 |
| serum dependent expression of enterococcus faecalis adhesins involved in the colonization of heart cells. | our previous studies have shown that the adhesive ability of enterococcus faecalis is dependent on the strain and is further modified by growth in serum. the data reported here demonstrate that e. faecalis adherence is mediated by carbohydrate residues present on the bacterial cell surface. some of these (d-mannose and d-glucose) are expressed by strains isolated from both urinary tract infections (uti) and endocarditis (en) when the cells are grown in brain-heart infusion broth (bhib), and medi ... | 1991 | 1795630 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ... | 1991 | 1798067 |
| cefpodoxime: comparative antibacterial activity, influence of growth conditions, and bactericidal activity. | the antimicrobial activity of cefpodoxime, the active metabolite of the new cephalosporin ester cefpodoxime proxetil, in comparison to cefixime, cefotiam, cefuroxime, and cefotaxime was determined against a broad spectrum of freshly isolated gram-positive and gram-negative bacterial strains. cefpodoxime was demonstrated to be inhibitory at concentrations of less than or equal to 1 mg/l against 90% of strains of moraxella catarrhalis, haemophilus influenzae, escherichia coli (beta-lactamase- nega ... | 1991 | 1800379 |
| time-kill kinetic studies of ampicillin/sulbactam for beta-lactamase-producing enterococci. | beta-lactamase-producing (bla+) enterococci have now been reported from several geographic areas. most of these strains also demonstrate high-level aminoglycoside resistance, making therapy of serious infections due to bla+ enterococci difficult. using time-kill kinetic studies, we evaluated the activity of ampicillin-sulbactam (am/sb) against five clinical bla+ enterococcus faecalis isolates from three geographically distinct areas. am at fourfold minimum inhibitory concentrations (mic) concent ... | 1991 | 1802536 |
| increasing resistance to beta-lactam antibiotics among clinical isolates of enterococcus faecium: a 22-year review at one institution. | to identify any change in the antibiotic resistance of enterococcus faecium, we examined the antibiotic susceptibilities of clinical strains (n = 84) isolated at one institution during the 22 years since 1968. a significant increase in resistance to penicillin was observed during the study period: the mics of penicillin for 50 and 90% of isolates tested were 16 and 64 micrograms/ml, respectively, from 1969 to 1988 (n = 48; geometric mean mic, 14 micrograms/ml) , whereas they were 256 and 512 mic ... | 1991 | 1803989 |
| crossover assessment of serum bactericidal activity and pharmacokinetics of ciprofloxacin alone and in combination in healthy elderly volunteers. | to better define the pharmacokinetics and serum bactericidal activity (sba) of ciprofloxacin and other antimicrobial agents in the elderly, six healthy (greater than 65 years) volunteers with normal renal function were given ciprofloxacin alone orally, ciprofloxacin plus rifampin orally, ciprofloxacin plus clindamycin orally, rifampin alone orally (three volunteers), and, for comparison of sba against gram-positive cocci, vancomycin intravenously. mean peak ciprofloxacin concentrations and other ... | 1991 | 1804008 |
| interaction of imipenem with erythromycin and tetracycline assessed by microdilution checkerboard techniques. | microdilution methodology was used to study the interaction of imipenem with erythromycin and tetracycline, a combination therapy that might be used for the treatment of serious pelvic inflammatory disease. the combination of imipenem and erythromycin showed no antagonism for escherichia coli and haemophilus influenzae but was antagonistic for staphylococcus aureus, enterococcus faecalis, and group b streptococci; the combination of imipenem and tetracycline was antagonistic for all strains exce ... | 1991 | 1804015 |
| [lactic acid production and urease activity in strains of enterococcus faecium found in the rumen and their genetic stability]. | lactic acid production, urease activity and genetic stability were investigated in five selected rumen strains of enterococcus faecium. the average value of urease activity in the tested strains was 16.5 +/- 0.953 nkat per ml, two strains were urease-negative. the values of e. faecium strains produced lactic acid ranged from 1.087 +/- 0.134 to 1.787 +/- 0.213 mmol per 1 l. cultivation of the strains in ethidium bromide (eb) eliminated urease activity of these strains already in the first subcult ... | 1991 | 1807029 |
| [phlegmon of the mouth floor with extremely severe course]. | a case of the 38-years old patient with a severe streptococcal phlegmon fundi oris and secondary deficiency of gamma-globulins was reported. the patient was successfully treated by surgery, proper antibiotico-therapy and immunoregulation. | 1991 | 1808588 |
| bacteria in the apical 5 mm of infected root canals. | ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ... | 1991 | 1809801 |
| antimicrobial activity of isepamicin (sch21420, 1-n-hapa gentamicin b) combinations with cefotaxime, ceftazidime, ceftriaxone, ciprofloxacin, imipenem, mezlocillin and piperacillin tested against gentamicin-resistant and susceptible gram-negative bacilli and enterococci. | isepamicin, formerly sch21420 or 1-n-hapa gentamicin b, is an aminoglycoside that was tested alone or in combination with one of seven broad spectrum drugs against 80 clinical isolates. half of the strains were gentamicin-resistant but only one isolate (1.3%) was resistant to isepamicin. the broadest spectrum comparison drugs tested alone (ciprofloxacin at 3.8% resistance and imipenem at 5.0% resistance) were associated with the lowest synergy rates when combined with isepamicin. the rank order ... | 1991 | 1809808 |
| distribution, antibiotic susceptibility and tolerance of bacterial isolates in culture-positive cases of endocarditis in the netherlands. | during a two-year period data were collected nationwide in the netherlands on 438 episodes of bacterial endocarditis (be) in 432 patients. of the strains isolated in these patients 419 were available for analysis. of these, 326 were isolated in native valve endocarditis (nve) and 93 in prosthetic valve endocarditis (pve). viridans streptococci, staphylococci and enterococci together constituted 87% of the isolates. more than 46% of the viridans streptococci consisted of streptococcus sanguis. en ... | 1991 | 1810724 |
| species identification and antibiotic resistance patterns of the enterococci. | one hundred and forty isolates were identified using the gpi card (vitek systems) and a conventional biochemical scheme. agar dilution susceptibility testing was performed and isolates were screened for beta-lactamase production. the gpi card was accurate in identifying enterococcus faecalis, but discrepant results were obtained for seven (29.1%) of the 24 non-enterococcus faecalis isolates. ampicillin resistance was present in 2.9% of isolates and there were no beta-lactamase producers. there w ... | 1991 | 1810727 |
| antimicrobial activity of ru29246 (hr916 metabolite) compared with four other oral beta-lactams tested against more than 5000 clinical isolates. | the activity of ru29246, the active metabolite of the oral cephalosporin ester hr916, was compared in a multicenter study with that of the four oral beta-lactam antibiotics cephalexin, cefaclor, cefixime and amoxicillin/clavulanate (amoxicillin/ca). ru29246 was generally 2- to 8-fold more active than the other oral cephalosporins and comparable to amoxicillin/ca against staphylococci, and was the most active cephalosporin against group b streptococci. all four cephalosporins were ineffective aga ... | 1991 | 1810737 |
| comparative in vitro activity of the new glycopeptide sk&f 104662 against problematic gram-positive bacteria. | the in vitro activity of the new glycopeptide antibiotic sk&f 104662 against problematic gram-positive microorganisms was determined using the agar dilution technique. for comparison, ampicillin, oxacillin, vancomycin, erythromycin and gentamicin were tested. sk&f 104662 was found to have high activity against staphylococcus aureus, coagulase-negative staphylococci (both methicillin sensitive and resistant strains), enterococcus faecalis, enterococcus faecium, listeria monocytogenes, corynebacte ... | 1991 | 1810738 |
| antibacterial activity of ciprofloxacin administered intravenously in a pharmacodynamic in vitro model. | the antibacterial activity of ciprofloxacin against gram-positive and gram-negative bacteria was evaluated in an in vitro model by simulating its pharmacokinetics following a single 100 or 200 mg administration intravenously. the one compartment open model described by grasso and coworkers was used for these experiments. ciprofloxacin concentrations in the model were reproduced by calculations based on the concentration-time curve in human volunteers as reported by wise and others. the maximum r ... | 1991 | 1813438 |
| susceptibility of enterococcus faecalis to twelve antibiotics, time-kill assays, and high-level aminoglycoside resistance in a university hospital in argentina. | a total of 201 enterococcus faecalis strains isolated from different body sites were tested to (i) establish their antibiotic susceptibility pattern; (ii) determine the percentage of strains highly resistant (mic greater than 2,000 micrograms/ml) to five aminoglycosides and (iii) know if the combination of penicillin or ampicillin plus an aminoglycoside is reliably synergistic for the strains with low-level resistance (mics ranged from the break point of susceptibility for each aminoglycoside to ... | 1991 | 1815269 |
| [the antimicrobial efficiency of aqueous solutions of calcium hydroxide on streptococcus mutans, streptococcus faecalis and candida albicans, in vitro]. | calcium hydroxide preparations ca(oh)2 are used as water solutions, putly, liner, cement or artificial material. the therapeutic effect of each medicaments depends on physical and chemical characteristic of additional components. the aim of the paper is to research the sensitivity of streptococcus faecalis and candida albicans to different water solution concentrations of calcium hydroxide. as solid plates, bacto-mitis-salivarius agar was used for s. mutans, torlak blood agar for s. faecalis and ... | 1991 | 1819950 |
| extracellular proteinase from enterococcus faecalis subsp. liquefaciens. i. growth and extracellular proteinase production under different culture conditions. | growth and extracellular proteinase production by enterococcus faecalis subsp. liquefaciens was studied on several culture media and under different incubation conditions. the organisms grew well and developed extracellular proteinase activity on proteinaceous media, but when it grew on collins basal medium (lacking of protein), growth was poor and proteinase activity was not detected. the activation energy for growth was estimated to be 116 kj/mol, the optimum being at 37 degrees c. proteinase ... | 1991 | 1821866 |
| extracellular proteinase from enterococcus faecalis subsp. liquefaciens. ii. partial purification and some technological important properties. | an extracellular proteinase from enterococcus faecalis subsp. liquefaciens has been purified 780-fold by a method including gel filtration on sephadex g-50 and affinity chromatography with gramicidin j as ligand. approximately 15% of the original enzyme activity was recovered. a purification of 14,800-fold, with 11.4% yield, may be reached using chromatofocusing as final step in the purification procedure. the molar mass of the enzyme has been estimated to be approximately 30 kda by sephadex gel ... | 1991 | 1821867 |
| [the na(+)-atpase and na+ circulation of streptococcus faecalis]. | | 1991 | 1828479 |