| rickettsial antibody in southern france: antibodies to rickettsia conorii and coxiella burnetii among urban, suburban and semi-rural blood donors. | three hundred and twenty-five sera from blood donors in the south of france were examined by means of the indirect fluorescent antibody test. 18% of the sera had antibodies to rickettsia conorii, with a significantly higher prevalence (26%) in urban and suburban areas than in semi-rural areas (13 to 16%). this supports the view that in the south of france the highest prevalence of mediterranean spotted fever is suburban. 5% of the sera had antibodies to coxiella burnetti in this area, in which q ... | 1987 | 3445329 |
| [two-dimensional contrast echocardiographic assessment of the time course of regional ischemic myocardial function]. | the time course of percent fractional area change (%fac) of the ischemic left ventricular wall as identified by myocardial contrast echocardiography was assessed. two-dimensional echocardiograms of the left ventricular short axis at the level of the chordae tendineae were recorded in 16 anesthetized open-chest dogs. myocardial ischemia was produced by occluding the left circumflex coronary artery (lcx) for 30 min, and identified by myocardial contrast echocardiography using aortic root contrast ... | 1987 | 3448165 |
| candida krusei infectious arthritis. a rare complication of neutropenia. | candida krusei infections are increasing in neutropenic patients. this is the first report of a case of c. krusei arthritis in a neutropenic leukemic patient. the organism colonized the patient's respiratory tract and most likely seeded the right knee by hematogenous spread. knee swelling and tenderness were minimal. joint fluid gram stain and fungal smears did not show the organism despite positive results on cultures. with therapy, the joint fluid converted from neutrophilic predominance to ly ... | 1987 | 3479022 |
| muscle precursor cells invade and repopulate freeze-killed muscles. | a problem with the use of muscle grafting as a therapeutic procedure is to produce a graft functionally adequate to replace a muscle of complex architecture, such as a sphincter muscle. we thought it might be possible to use dead cadaver muscles, repopulated by the patient's own muscle precursor cells (mpc), to reconstruct muscles whose anatomy would be imposed by the framework of dead muscle and whose genetic constitution would be determined by the mpc. here we show, in the mouse, that an exten ... | 1987 | 3480896 |
| human gene mapping 9. paris conference (1987). ninth international workshop on human gene mapping. | | 1987 | 3484355 |
| energy-dependent uptake of n-methyl-4-phenylpyridinium, the neurotoxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, by mitochondria. | 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp), an impurity in certain batches of illicit heroin substitutes, is known to cause parkinsonian symptoms and degeneration of the nigrostriatal cells in drug abusers and primates. neurotoxicity depends on oxidation of mptp by monoamine oxidase in brain cells to the dihydropyridinium form, which is further oxidized to n-methyl-4-phenylpyridinium (mpp+), the 4-electron oxidation product. the latter is widely believed to be the compound responsible f ... | 1986 | 3486869 |
| relationships between pulmonary inflammation, plasma transudation, and oxygen metabolite secretion by alveolar macrophages. | we have previously shown that alveolar macrophages from normal rabbit lungs do not synthesize reactive oxygen intermediates unless first conditioned by culture in vitro in the presence of serum for 24 to 48 hr. this conditioning process is mediated by a serum constituent that partitions on gel exclusion columns with an apparent m.w. of 30,000 to 50,000 daltons. alveolar macrophage conditioning in vitro requires protein synthesis, is associated with the generation of membrane nadph oxidase activi ... | 1986 | 3486910 |
| corticotropin-releasing factor disrupts sensory responses of brain noradrenergic neurons. | in order to elucidate the possible role of noradrenergic neurons of the nucleus locus coeruleus (lc) in stress responses, the effects of corticotropin-releasing factor (crf) on lc neuronal activity were characterized. in haloth-aneanesthetized rats, intracerebroventricular administration of crf was found to have two distinct actions: a dose-dependent increase in spontaneous discharge activity was observed 3 min after peptide injection, with 1.0 and 3.0 micrograms crf increasing activity by 7 +/- ... | 1987 | 3492683 |
| accumulation of kupffer cells within lymphocyte aggregates in the livers of chickens injected with colloidal carbon and erythrocytes. | | 1987 | 3500351 |
| an unexpected q fever endocarditis. report of a case. | a man with blood culture negative endocarditis since 1983 received in october 1984 a mitral valve bioprosthesis. reintervention in april 1985 was performed because of a paravalvular leak. in september 1985, mitral insufficiency reappeared and high-titer phase ii q fever antibody was detected, which has since then persisted with concomitant high-titers of phase i antibody. in serum from 1983, phase ii antibody was detected on reexamination in september 1985. for unexplained reasons this antibody ... | 1987 | 3501654 |
| prognostic value of purified protein derivative skin test and granuloma formation in patients treated with intravesical bacillus calmette-guerin. | we evaluated the prognostic value of purified protein derivative skin test reactivity and a granulomatous response in intravesical bacillus calmette-guerin therapy. we treated 62 patients with intravesical bacillus calmette-guerin once a week for 6 weeks. purified protein derivative skin tests were performed before and after therapy. cold cup bladder biopsies were examined in a blind retrospective manner for the presence of granulomas 6 weeks after completion of therapy. a significant correlatio ... | 1986 | 3511285 |
| surfactant-anti-surfactant immune complexes in infants with respiratory distress syndrome. | the authors sought to determine whether treatment of respiratory distress syndrome (rds) with human surfactant resulted in the formation of detectable circulating immune complexes. preterm infants with severe rds were divided into two groups: one group received human surfactant by intratracheal instillation and the other group did not. both groups received ventilatory management involving intermittent mandatory ventilation. plasma samples were drawn from these babies prior to treatment and at in ... | 1986 | 3511728 |
| immunoenzymatic test for q-fever. | an immunoenzymatic test using as antigen purified suspensions of coxiella burnetti coated by methylglyoxal on microtiter plates was developed. multiple testing of the same sera gave similar results: two dilutions of serum (1:400 and 1:1600) were used in routine tests. good agreement between the immunoenzymatic and the indirect immunofluorescent antibody tests was obtained for 41 of 50 sera examined. five sera negative by the immunofluorescent antibody test were positive by the immunoenzymatic te ... | 1986 | 3514100 |
| [seroprevalence of human q fever in switzerland]. | in current practice, only serology, by the titration of anti-coxiella burnetii antibodies, permits the diagnosis of acute or chronic q fever. new serological techniques have also made it possible to more accurately determine the number of people in a community who have been affected by this infectious agent. we have thus been able to compare the results of 1437 specimens from the whole of switzerland with 4009 taken in the valais. the analysis of these sera by indirect microimmunofluorescence no ... | 1986 | 3520805 |
| androgen resistance in man. | | 1986 | 3521221 |
| rickettsial perimyocarditis--a follow-up study. | sera and lymphocytes from a 37-year-old male patient with acute perimyocarditis during a q-fever endemic were analyzed for antibody and cell-mediated immune reactions and followed up 28 months later. circulating autoantibodies against myocardial tissue were assessed by indirect immunofluorescence. cytolysis of vital contracting rat cardiocytes, by antimyolemmal antibodies and complement, and lymphocytotoxicity, with and without the patient's serum, were evaluated and compared with the results ob ... | 1986 | 3522535 |
| effects of glucose, insulin and epinephrine on lactate metabolism by isolated adipocytes. | epididymal fat cells were incubated in vitro with either 2 mm or 6 mm [u-14c]lactate, and where appropriate, 6 mm glucose, 10 mu/ml insulin or 2 micrograms/ml epinephrine. increasing lactate concentration promoted lactate carbon incorporation into specific products, and the presence of glucose significantly enhanced this effect. significant lipogenic or lipolytic hormonal effects upon lactate metabolism were observed only in the presence of glucose, as insulin primarily increased lactate convers ... | 1986 | 3526480 |
| [tolerance and immunization trials with a commercial vaccine against chlamydia psittaci and coxiella burnetii]. | | 1986 | 3527655 |
| the carrier state: group b streptococcus. | | 1986 | 3528112 |
| insulin and glucose responses to glucose injection in fed and fasted obese and lean sheep. | effects of short-term fasting on the insulin and glucose responses to injected glucose were determined in obese (n = 6) and lean (n = 6) dorset ewes that were fed a maintenance level of energy intake. sheep were assigned by latin-square design to be fasted for 0 (fed), 12 or 24 h before glucose (350 mg/kg) was injected via jugular cannula at 2000 h with at least 7 d between successive tests. insulin and glucose were quantified in jugular plasma samples. pretreatment concentrations of insulin wer ... | 1986 | 3528428 |
| preferential alcoholic embryopathy among contiguous siblings of long-evans rats. | ethanol exposure alters sex steroidogenesis and sexually dimorphic behaviors in rodent offspring. contiguity to siblings of the same or opposite sex in utero also affects steroidogenesis and sexual dimorphism in rodents. the present study with long-evans rats shows that maternal exposure to ethanol during the critical period of rodent organogenesis preferentially affects body weights and increases malformations in offspring dependent on their in utero contiguity to siblings of the opposite sex. | 1986 | 3530015 |
| [immunofluorescence for the rapid diagnosis and complex study of various acute and chronic respiratory infections]. | direct and indirect immunofluorescence techniques were used to investigate the presence of some viral, chlamydial, rickettsia and mycoplasma antigens in the exfoliated nasal and pharyngeal epithelial cells from 156 patients with acute or chronic respiratory infections. positive results were found in 98 (62.82%) of them, parainfluenza (36.88%) and herpes (23.3%) antigens, being the most frequent. herpes, parainfluenza and adenoviral antigens were found the most frequently among patients with phar ... | 1986 | 3532518 |
| multiplication-stimulating activity-induced alkalinization of canine renal proximal tubular cells. | the actions of a variety of polypeptide growth factors on isolated cells are thought to be initiated by stimulation of na+-h+ exchange across the plasma membranes of the cells resulting in intracellular alkalinization. to determine whether insulin-like growth factors (igfs) exert actions through such a mechanism, we incubated suspensions of canine renal proximal tubular segments with insulin or igf i or with multiplication-stimulating activity (msa)/igf ii. changes in intracellular ph were detec ... | 1986 | 3533920 |
| propionibacterium acnes cerebral botryomycosis. the role of plastic embedding in the diagnosis of grain-producing infections. | botryomycosis is a chronic infection in which bacterial colonies, or "grains," occur in purulent exudate associated with draining sinus tracts or abscess formation. this condition usually is caused by staphylococcus and streptococcus, but other organisms occasionally have been implicated. this report describes the first case of botryomycosis caused by propionibacterium acnes, a diphtheroid that has been rarely associated with opportunistic disease. in this case, plastic embedding allowed the obs ... | 1986 | 3535486 |
| bacterial beta-lyase mediated cleavage and mutagenicity of cysteine conjugates derived from the nephrocarcinogenic alkenes trichloroethylene, tetrachloroethylene and hexachlorobutadiene. | the metabolism of beta-lyase and the mutagenicity of the synthetic cysteine conjugates s-1,2-dichlorovinylcysteine (dcvc), s-1,2,2-trichlorovinylcysteine (tcvc), s-1,2,3,4,4-pentachlorobuta-1,3-dienylcysteine (pcbc) and s-3-chloropropenylcysteine (cpc) were investigated in salmonella typhimurium strains ta100, ta2638 and ta98. the bacteria contained significantly higher concentrations of beta-lyase than mammalian subcellular fractions. bacterial 100,000 x g supernatants cleaved benzthiazolylcyst ... | 1986 | 3536138 |
| chlamydial infections. | | 1986 | 3536193 |
| studies on the mechanics of the normal human middle ear. | the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ... | 1986 | 3536194 |
| humoral immune response to q fever: enzyme-linked immunosorbent assay antibody response to coxiella burnetii in experimentally infected guinea pigs. | the response of guinea pigs experimentally infected with coxiella burnetii organisms, the etiologic agents of q fever, was obtained by the measurement of fever, circulating infectious c. burnetii cells, and anti-c. burnetii antibodies. the detection of antibodies by the enzyme-linked immunosorbent assay (elisa) and traditional methods against phase i whole cells, phase ii whole cells, and phase i lipopolysaccharide (lps-i) (a virulence marker for phase i cells) antigens in the serum samples of i ... | 1986 | 3537005 |
| colloidal gold-labeled insulin complex. characterization and binding to adipocytes. | biologically active insulin gold complex was used as an ultrastructural marker to study insulin binding sites, uptake, and internalization in isolated rat adipocytes. the preparation conditions for monodispersed particles, ca. 16 nm in diameter and loaded with approximately 100 insulin molecules, are reported. the complex is stable for at least six weeks. single particles or small clusters were scattered across the cell membrane. the distribution of unbound receptors seemed to be independent of ... | 1986 | 3539893 |
| biochemical and functional characterization of the rat liver glucose-transport system. comparisons with the adipocyte glucose-transport system. | the properties of the glucose-transport systems in rat adipocytes and hepatocytes were compared in cells prepared from the same animals. hormones and other agents which cause a large stimulation of 3-o-methylglucose transport in adipocytes were without acute effect in hepatocytes. hepatocytes displayed a lower affinity for 3-o-methylglucose (20 mm) and alternative substrates than adipocytes (6 mm), whereas inhibitor affinities were similar in both cell types. the concentration and distribution o ... | 1986 | 3548706 |
| serological diagnosis of q fever endocarditis. | the diagnosis of q fever endocarditis cannot be made by bacterial cultures and necessitates serological identification of specific antibodies to coxiella burnetii which stimulates mainly the production of anti-phase ii antibodies during the acute disease, but primarily anti-phase i antibodies in endocarditis. indirect microimmunofluorescence allows rapid detection of specific iga, igg and igm. the results of serological analyses of 191 acute cases of q fever were compared with those of 8 cases o ... | 1986 | 3549313 |
| q fever in the netherlands: a sero-epidemiological survey among human population groups from 1968 to 1983. | a sero-epidemiological survey, using an indirect immunofluorescence test for igg against coxiella burnetii (phase ii), was carried out in the netherlands. serum samples taken in 1968, 1975, 1979 and 1983 were tested. occupational groups with a supposedly high risk of infection (veterinarians, residents of dairy farms and taxidermists) showed a significantly higher percentage of seropositives than defined controls. the percentage of seropositive amateur wool spinners was significantly higher than ... | 1987 | 3549343 |
| limited in vitro multiplication of mycobacterium leprae: application to screening potential antileprosy compounds. | | 1986 | 3553808 |
| peripheral hepatic arterial embolization with crosslinked collagen fibers. | hepatic artery embolization with a nonimmunogenic, crosslinked microfibrillar collagen preparation (angiostat, collagen for embolization, target therapeutics, los angeles, ca) was studied in mongrel dogs. flow-directed technique was used to achieve complete distal arterial occlusion. serial liver function evaluation demonstrated marked alterations at 48 to 72 hours, partial correction at one week, and resolution of abnormalities by one month. restoration of large vessel blood flow was angiograph ... | 1987 | 3557884 |
| structure and biological relationships of coxiella burnetii lipopolysaccharides. | lipopolysaccharides (lpss) extracted from nine strains of coxiella burnetii were analyzed for chemical compositions, molecular heterogeneity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and lethal toxicities in galactosamine-sensitized mice. the structure of a unique disaccharide of hydrolyzed phase i lps was determined to be galactosaminuronyl-alpha (1-6)-glucosamine (galnu-alpha (1-6)-glcn, c12h22n2o10) with an mr of 354. the mr of lpss of c. burnetii intra- and interspecific ... | 1987 | 3558367 |
| isolation of lipopolysaccharide of coxiella burnetii & some of its effects on guineapigs. | | 1986 | 3570386 |
| comparative virulence of intra- and interstrain lipopolysaccharide variants of coxiella burnetii in the guinea pig model. | we compared the relative infectivity and virulence of lipopolysaccharide (lps) variants of the nine mile strain of coxiella burnetii with those of the priscilla strain, a representative of endocarditis-type strains. in agreement with results of previous studies, nine mile phase i (9mi/i) organisms were highly infectious, eliciting seroconversion and fever with inocula containing as few as four organisms. viable 9mi/i was recovered from the spleens of infected animals 30 days postinfection. nine ... | 1987 | 3570458 |
| redistribution of microtubules and pericentriolar material during the development of polarity in mouse blastomeres. | the distribution of microtubules and microtubule organizing centers (mtocs) during the development of cell polarity in eight-cell mouse blastomeres was studied by immunofluorescence and immunoelectron microscopy using monoclonal anti-tubulin antibodies and an anti-pericentriolar material (pcm) serum. in early eight-cell blastomeres microtubules were found mainly around the nucleus and in the cell cortex, whereas pcm foci were observed dispersed in the cytoplasm. during the eight-cell stage, micr ... | 1987 | 3571331 |
| conditioned taste aversion in vasopressin-deficient rats (brattleboro strain). | brattleboro rats are homozygous for diabetes insipidus (di), lacking the ability to synthesize vasopressin. previous studies reported learning deficits in di rats on passive avoidance tasks using footshock. other studies, however, could not replicate these results. in two experiments, we studied the learning of di and control long evans (le) rats in a different avoidance paradigm: conditioned taste aversion (cta). in the first experiment a mild cta to saccharin was established gradually using lo ... | 1987 | 3575495 |
| [serologic reactions in humans during the outbreak of q fever]. | | 1986 | 3575758 |
| [2 foci of q fever in humans, sheep and cattle in the olsztyn province]. | | 1986 | 3575759 |
| expression of cdnas for acetylcholine receptor subunits in the yeast cell plasma membrane. | yeast cells transformed with a plasmid containing cdna encoding the alpha or delta subunit of the torpedo californica acetylcholine receptor synthesize a protein. the expected molecular mass, antigenic specificity, and ligand-binding properties (in the case of the alpha subunit) of the subunits in yeast are similar to those of the subunits in t. californica membranes. the subunits are inserted into the yeast plasma membrane, demonstrating for the first time that yeast has the apparatus to expres ... | 1986 | 3579969 |
| expression of cdnas for acetylcholine receptor subunits in the yeast cell plasma membrane. | yeast cells transformed with a plasmid containing cdna encoding the alpha or delta subunit of the torpedo californica acetylcholine receptor synthesize a protein. the expected molecular mass, antigenic specificity, and ligand-binding properties (in the case of the alpha subunit) of the subunits in yeast are similar to those of the subunits in t. californica membranes. the subunits are inserted into the yeast plasma membrane, demonstrating for the first time that yeast has the apparatus to expres ... | 1986 | 3579969 |
| effects of nonsteroidal antiinflammatory agents (nsaias) on wound healing. | | 1986 | 3583327 |
| an outbreak of q fever affecting postal workers in oxfordshire. | twenty-five people in oxfordshire were found to have had clinical illness due to q fever in the 3 months from april to june 1983. twelve cases were diagnosed through the routine laboratory diagnostic service. five of these were postmen, four of whom worked in a sorting office where an outbreak of illness similar to influenza had been noted by the occupational health nurse. thirteen cases were diagnosed by active case-finding in this sorting office but investigation failed to define the source of ... | 1987 | 3585037 |
| analysis of q fever in uruguay. | the first outbreak of q fever in uruguay occurred in 1956. the infection, which is caused by coxiella burnetti, occurs in sheep, cattle, swine, and horses, but not in fowl or guinea pig. most of the cases studied have been traced to cattle. fourteen outbreaks of this disease were studied between 1975 and 1985. all of the 1,358 clinically suspected cases (814 serologically confirmed) reported in uruguay occurred in workers at meat-processing plants. diagnoses of cases and serologic surveys were m ... | 1987 | 3589335 |
| perivascular and tissue po2 in contracting rat spinotrapezius muscle. | this study evaluated the possibility that during skeletal muscle contractions tissue o2 tension (po2) around arterioles and venules decreases substantially more than in the middle of the capillary bed and thereby influences functional hyperemia. periarteriolar [h+] and [k+] were also measured because most large arterioles are in close proximity to venules such that the biochemical status of the periarteriolar tissue could be influenced by a large decrease in o2 availability in the annulet of tis ... | 1987 | 3591968 |
| atypical pneumonia: recognition and treatment. | while the term "atypical pneumonia" has been in use for many years, it cannot in fact be defined. however, there is a persuasive reason to retain the clinical use of the term, and that is to provide a guide for the clinician in the choice of empirical antibiotic therapy for patients with acute pneumonia. atypical pneumonia, then, is a descriptive term for a common clinical syndrome. provided certain clinicoepidemiological groups are excluded, the most common infectious causes of this syndrome ar ... | 1987 | 3600471 |
| can calcium antagonists affect the hemostatic mechanism and promote rebleeding? an experimental study. | calcium antagonists are currently under investigation as potential beneficial drugs in the prevention of cerebral vasospasm. however, some calcium antagonists have the propensity to inhibit platelet aggregation. this study investigated the danger posed by the administration of calcium antagonists for recurrent hemorrhage in a simulated rat model of subarachnoid hemorrhage. we found that verapamil, a calcium antagonist with antiplatelet activity, lowers clot resistance and may promote rebleeding. | 1987 | 3601019 |
| the pathology of q fever hepatitis. a case diagnosed by liver biopsy. | a 52-year-old woman with fever and low grade hepatitis had q fever, a diagnosis made by the characteristic granulomas, containing fibrin and vacuoles, on liver biopsy and confirmed by serology. fibrin was demonstrated in the granulomas but coxiella burnetii antigens were not. the literature on the histopathology of the liver in both acute q fever and q fever endocarditis shows that none of the 220 cases of q fever endocarditis, with one possible exception, had the characteristic granulomas of ac ... | 1987 | 3611692 |
| [pathomorphological studies of aborted fetuses from sheep infected with r. burneti]. | serologic investigations were carried out with 441 samples of blood sera from ewes, coupled with histopathologic studies of 18 aborted fetuses. positive serologic q fever results were obtained by the cf-test with 14.4 per cent of the sheep. abortions were ascertained with 4 to 8 per cent of the animals per flock in the final month of pregnancy. nonviable lambs were also obtained that died in the course of the first hours after lambing. the gross lesions observed consisted in the swelling and hyp ... | 1987 | 3617464 |
| [acid-base equilibrium in calves with enteritis]. | studied were the indices of the acid-alkaline balance and the lactate level in a total of 19 black-and-white calves manifesting clinical forms of enteritis as well as in 10 clinically normal calves that belonged to the same breed. in most of the calves affected with enteritis the blood ph values proved essentially lower than those found in normal calves. differences were established between the normal and the diseased animals with regard to abe, hco-3, and pco2, the abe values with some of the a ... | 1987 | 3617465 |
| effects of methylprednisolone on collagen biosynthesis in healing acute myocardial infarction. | | 1987 | 3618516 |
| enhanced 2-deoxyglucose incorporation in peripheral nerve during ischemia. | we examined the effect of acute ischemia on peripheral nerve uptake of the glucose analog 2-deoxyglucose (2-dg). endoneurial 2-dg incorporation was uniform at rest, but increased focally in areas subjected to moderate levels of ischemia which were not severe enough to impair nerve conduction. we believe these data are indicative of increased endoneurial glucose metabolism probably reflecting a compensatory shift to less efficient anaerobic glycolysis. this mechanism may in part account for perip ... | 1987 | 3620935 |
| [preliminary study on the identification of coxiella burnetii isolated in china by pyrolysis gas chromatography]. | | 1987 | 3630146 |
| [on man and nursing. social and ethnological aspects of human musicality]. | | 1987 | 3648346 |
| q fever inflammatory pseudotumor of the lung. | a 36-year-old man presented with malaise, myalgia, fever, and weight loss. liver function tests were moderately abnormal and chest x-ray film revealed an irregular mass in the right lower lobe. bronchoscopy with cytology was not helpful. serology revealed markedly elevated titers to q fever. the mass was presumed to represent the presence of a q fever pseudotumor. the patient responded to therapy with resolution of symptoms and chest x-ray film and decrease in titers to q fever. | 1987 | 3652767 |
| antigen-specific circulating immune complexes in coxiella burnetii-infected guinea pigs. | an antigen-specific conglutinin-binding assay was developed with artificial immune complexes of coxiella burnetii and an enzyme-linked immunosorbent assay for the detection of circulating immune complexes. sera from guinea pigs infected with c. burnetii were examined by this assay, and the percentages of infected guinea pigs with c. burnetii antigen-specific circulating immune complexes were 71, 93, 74, 65, 49, and 27%, respectively, from the first to sixth week after infection. c. burnetii anti ... | 1987 | 3653345 |
| factors affecting control of fannia spp. (diptera: muscidae) with cyromazine feed-through on caged-layer facilities in southern california. | | 1987 | 3655087 |
| animal models in q fever: pathological responses of inbred mice to phase i coxiella burnetii. | the susceptibility of inbred strains of mice to infection by phase i coxiella burnetii, the aetiological agent of q fever, was investigated by evaluating morbidity, mortality, antibody production and in vitro proliferative responses of splenic lymphocytes. among the 47 strains of mice tested for morbidity and mortality to c. burnetii infection, 33 were resistant, 10 were of intermediate sensitivity, and four were sensitive. a/j mice exhibited the highest mortality, and surviving mice of this str ... | 1987 | 3655728 |
| isoperiodic bursting by magnocellular neuroendocrine cells in the rat hypothalamic slice. | 1. recruitment of magnocellular neuroendocrine cells (m.n.c.s) to a repetitive burst pattern (phasic firing) is associated with increased vasopressin secretion from neurohypophysial terminals in the intact animal. based on invertebrate studies, bursts of action potentials can arise in two distinct ways: as an intrinsic property of the recorded cell or as an emergent property of synaptic interactions. 2. the majority of phasic m.n.c.s in the hypothalamic slice preparation display an endogenous pa ... | 1987 | 3656153 |
| [the nature of forces stabilizing nucleosome structure. dissociation of histone octamers from dna]. | we have used the measurements of the histone fluorescence parameters to study the influence of the ionic strength on histone-dna and histone-histone interactions in reconstructed nucleosomes. the ionic strength increase lead to the two-stage nucleosome dissociation. the dimer h2a-h2b dissociates at the first stage and the tetramer (h3-h4)2 at the second one. the dimer h2a-h2b dissociation from nucleosome is a two-stage process also. the ionic bonds between (h2a-h2b) histone dimer and dna break a ... | 1987 | 3657773 |
| analysis of caprine igg1 and igg2 subclass responses to chlamydia psittaci infection and vaccination. | enzyme-linked immunosorbent assays (elisas) specific for caprine igg(h+l), igg1 and igg2 were developed and evaluated for serodiagnosis of chlamydia psittaci infections in a tunisian goat flock with currently occurring chlamydial abortions and a clinically inapparent goat flock of an animal research facility. additionally, elisas were applied to record the igg1 and igg2 dynamics of four goats vaccinated with inactivated chlamydia psittaci and coxiella burnetii. for screening purposes, the igg(h+ ... | 1987 | 3660580 |
| in vitro susceptibility of coxiella burnetii to antibiotics, including several quinolones. | antibiotic susceptibility testing of the rickettsial q fever agent coxiella burnetii was performed by using persistently infected l929 fibroblast cells. the efficacies of a variety of antibiotics with different metabolic targets were tested and compared. the most effective antibiotics in bringing about the elimination of the parasite from infected cells included several quinolone compounds and rifampin. of the quinolone compounds tested, difloxacin (a-56619) was the most effective, followed by c ... | 1987 | 3662472 |
| effects of temperature upon the cell-free translation system from coxiella burnetii. | the rate and extent of coliphage q beta rna translation by cell-free extracts prepared from coxiella burnetii were studied. when translations were conducted at temperatures elevated above 37 degrees c, both polypeptide elongation and frequency of initiation were by comparison increased. the ratios of products synthesized from the polycistronic phage mrna also changed upon increases in translation temperature, especially at 45 degrees c. although the organism is a moderate acidophile, initiation ... | 1987 | 3664368 |
| micromethod for the determination of cefpiramide in human plasma and urine by high-performance liquid chromatography using automated column switching. | | 1987 | 3667797 |
| lipid laden macrophages in synovial fluid: a late finding in traumatic arthritis. | a 66-year-old man presented with arthritis of the right knee of 3 months' duration. cytologic examination of synovial fluid (sf) showed numerous lipid laden macrophages. radiographs and a nuclear scintigram established the presence of a fracture of the lateral tibial plateau. the diagnostic significance of lipid laden macrophages in sf is reviewed. | 1987 | 3668993 |
| [evaluation of the results of serologic studies of persons from a focus of q fever]. | | 1987 | 3669802 |
| [electron microscopic methods for the rapid diagnosis of q fever in animals]. | methods have been worked out for the electron-microscopic diagnosis of q-fever. a characteristic feature with them is the use of clinical, pathological, and experimental material that is investigated through negative contrast and ultra thin sections for the detection of rickettsial cells and inclusions. it is underlined that the methods referred to are readily applicable, economic, highly effective, and hazardless. they are described for the first time and could be adopted into the diagnostic an ... | 1987 | 3672902 |
| stereoselective hydrolysis of soman in human plasma and serum. | the contribution of various human serum and plasma fractions to the total hydrolysis rate constants of the four isomers of soman is studied. spontaneous hydrolysis (as measured in buffer) occurs at a faster rate for the c(+)p(+)- and c(-)p(-)-isomers. a stereoselectively catalyzed hydrolysis of soman occurs in serum fractions iv and v (albumin). in fraction v the c(+)p(+)- and c(-)p(-)-isomers are hydrolyzed at a faster rate than their respective epimers, while in fraction iv-1 a stereoselective ... | 1987 | 3675614 |
| a gdp-fucose:[gal beta 1----4]glcnac alpha 1----3-fucosyltransferase activity is correlated with the presence of human chromosome 11 and the expression of the lex, ley, and sialyl-lex antigens in human-mouse cell hybrids. | by fusion of human leukocytes and cells of the murine myeloid cell line wehi-tg, we produced human-mouse myeloid cell hybrids. hybrids which contain human chromosome 11 have been demonstrated to express the myeloid-associated carbohydrate antigen lex (geurts van kessel, a. h. m., tetteroo, p. a. t., von dem borne, a. e. g. kr., hagemeijer, a., and bootsma, d. (1983) proc. natl. acad. sci. u. s. a. 80, 3748-3752). in this paper, we report that the hybrids that contain chromosome 11 also expressed ... | 1987 | 3680240 |
| effect of verapamil on cholesteryl ester hydrolysis and reesterification in macrophages. | the macrophage-like cell line, j774, and its variant, ct2, were used to study the effect of verapamil on metabolism of esterified cholesterol. the cells had been labelled with 3h-cholesterol for 24 hours and thereafter acetylated low density lipoprotein (ldl) or d less than 1.019 g/ml fraction of hypercholesterolemic rabbit plasma was added. after an additional 24 hours, 60% or 40% of the label, respectively, was recovered in esterified cholesterol in control dishes, but only 2% to 15%, in the p ... | 1987 | 3689204 |
| [change in tissue glycogen reserves in tumor bearers as a reflection of a hypoglycemic stress syndrome]. | glycogen content in the brain, liver and skeletal muscles of rats bearing ascite zajdela hepatoma (azh) and solid 27 hepatoma (27-h) has been studied. serum glucose levels directly correlated with liver glycogen reserves. in the terminal stage of tumor growth depletion of liver glycogen was observed, while the stores of muscle glycogen did not diminish. within 1-4 days (azh) and 15-30 days (27-h) after implantation the stores even exceeded those of control healthy rats. in the terminal stage, in ... | 1987 | 3689968 |
| intracellular recordings from pineal cells in tissue culture: membrane properties and response to norepinephrine. | membrane properties and responsiveness to norepinephrine were studied in pinealocytes in tissue culture using both conventional and whole-cell patch-clamp techniques. action potentials could be recorded in normal and tetrodotoxin-containing saline. both outward and inward rectification were prominent. under voltage-clamp, depolarizing voltage steps evoked time-dependent outward currents showing no inactivation over time. hyperpolarizing steps evoked slowly developing inward currents which did no ... | 1986 | 3697732 |
| dehydration increases the electrically evoked dopamine release from the neural and intermediate lobes of the rat hypophysis. | the effect of dehydration on dopamine (da) release from the neural and intermediate lobes of the rat pituitary gland after electrical stimulation of the pituitary stalk was studied in vitro. the amount of da released from pituitary lobes of dehydrated rats (no water for 72 h), in which the hormone secretion from the hypophysis, especially the neural lobe, is known to be greatly enhanced, was significantly higher when compared with that from control tissues. in control and dehydrated rats the evo ... | 1986 | 3713989 |
| use of inversion spin transfer to monitor creatine kinase kinetics in rat skeletal muscle in vivo. | a simple multipulse sequence has been used to monitor creatine kinase kinetics in rat skeletal muscle in vivo. using these procedures, the forward (atp synthesis) and reverse fluxes (phosphocreatine synthesis) have been calculated to be 8.98 +/- 0.6 and 10.7 +/- 0.8 mumoles/g wet wt/s (n = 5) respectively. these results suggest that in resting skeletal muscle most of the gamma atp observed in 31p nmr spectra is cytosolic and rapidly exchanging with phosphocreatine. the high flux rates reflect th ... | 1986 | 3718523 |
| morphogenesis of sclerotome and neural crest in avian embryos. in vivo and in vitro studies on the role of notochordal extracellular material. | the distribution of sclerotome and neural crest cells of avian embryos was studied by light and electron microscopy. sclerotome cells radiated from the somites towards the notochord, to occupy the perichordal space. neural crest cells, at least initially, also entered cell-free spaces. at the cranial somitic levels they moved chiefly dorsal to the somites, favouring the rostral part of each somite. these cells did not approach the perichordal space. more caudally (i.e. trunk levels), neural cres ... | 1986 | 3719666 |
| tachycardic and hypertensive effects of centrally administered clonidine in conscious rats. | the centrally mediated cardiovascular changes induced by clonidine were studied in conscious rats. clonidine administered intracerebroventricularly (i.c.v.), and intravenously (i.v.) caused hypotension following an initial pressor response. i.v. clonidine caused significant greater hypotension than i.c.v. clonidine (30 micrograms/kg; p less than 0.05). with the 30 micrograms/kg i.c.v. dose, a tachycardia was observed in all rats following initial transient bradycardia. no tachycardia was observe ... | 1986 | 3720002 |
| aortic changes induced by hypercholesterolemia and hypercalcemia in rats. | the effects of vitamin d and cholesterol-rich diets on rat aortas were examined. examination by electron microscopy showed a widening of the subendothelial space and the presence in this space of amorphous substances as early as the second week into mild hypercholesterolemia. after further time elapsed, basement membrane-like substances, microfilaments, migration of mononuclear cells, and modified smooth muscle cells were seen in the subendothelial space. in the vitamin d group, the main electro ... | 1986 | 3720918 |
| kawasaki syndrome: a controlled study of an outbreak in wisconsin. | the etiology of kawasaki syndrome remains unestablished, although a possible role has been suggested for exposure to the application of carpet shampoo, house dust mites, and rickettsial infection. during an outbreak of 20 cases of kawasaki syndrome that occurred in southeastern wisconsin from november 1982 through march 1983, a case-control study was done of 15 cases and 30 matched controls. the study included questionnaire administration, dust collection from homes, and serum specimen collectio ... | 1986 | 3728445 |
| q fever endocarditis. | q fever endocarditis, which is seen most often in great britain and australia, has been rarely observed in the united states. a patient with an eight month febrile illness who had signs and symptoms of endocarditis and serologic studies diagnostic of q fever endocarditis is reported. a history of extensive travel makes it unclear where he originally contracted the disease. q fever endocarditis is probably underdiagnosed and should be looked for in any case of culture negative endocarditis or chr ... | 1986 | 3728557 |
| q fever vaccination of sheep: challenge of immunity in ewes. | adult ewes (17 months of age) were vaccinated against coxiella burnetii, using a formalin-inactivated whole cell (wc) phase i henzerling strain vaccine or a chloroform methanol residue (cmr) vaccine. nineteen pregnant ewes were placed in 3 categories [(i) unvaccinated, (ii) wc vaccine, and (iii) cmr vaccine] and were challenge exposed at approximately the 100th day of gestation with 210,000 plaque-forming units of c burnetii inoculated subcutaneously. shedding of rickettsiae was measurably reduc ... | 1986 | 3729123 |
| environmental risk assessment of industrial facilities: techniques, regulatory initiatives and insurance. | most companies that manufacture, handle or dispose of chemicals or petroleum products have the potential to cause environmental impairment, especially by longterm or gradual release of materials into the environment. this impairment creates potential liabilities that result from numerous regulations as well as from common law. one way to identify these potential environmental liabilities and exposures is through environmental risk assessment. the primary focus of an environmental risk assessment ... | 1986 | 3738490 |
| [analysis of the layer distribution of transcallosal responses in the sensorimotor cortex of kittens in the 1st month of life]. | in experiments on 23 kittens ageing between 1-30 days, anaesthetized by barbiturate and immobilized by diplacine, studies have been made on the distribution of transcallosal responses (tcrs) along the whole diameter of the sensorimotor cortex. in order to obtain more accurate data on localization of the sources and drains of extracellular current vertical component, a method of double graphic differentiation was used. it was shown that in 1-2-day kittens tcrs have a positive oscillation form thr ... | 1986 | 3739466 |
| encephalitis caused by coxiella burnetii. | acute infection with coxiella burnetii usually results in a self-limited illness, but it can occasionally cause chronic endocarditis or hepatitis. headache is a common presenting symptom of acute infection with this agent, but specific neurological abnormalities are rare. we report the case of a patient with acute q fever that caused frank encephalitis. we also review the literature on central nervous system disease attributable to c. burnetii. | 1986 | 3740816 |
| development of the apical ectodermal ridge and changes in the subepidermal mesenchyme in mouse limb buds. | | 1986 | 3749201 |
| [toxicity of mercury from yeast biomasses in albino rats]. | | 1986 | 3751187 |
| [reactogenic properties and immunogenicity of measles and mumps vaccines when used by different procedures]. | in the controlled epidemiological trial the reactogenic and immunogenic properties of live measles vaccine (lmv) and live parotitis vaccine (lpv) were evaluated in identical groups of children immunized in accordance with different schedules: only lmv or lpv, the injection of these preparations mixed in one syringe or with the use of separate syringes, or at an interval of 3 months. the highest number of febrile reactions, rash and catarrhal symptoms was registered among children immunized with ... | 1986 | 3751401 |
| [reactogenic properties and immunogenicity of measles and mumps vaccines when used by different procedures]. | in the controlled epidemiological trial the reactogenic and immunogenic properties of live measles vaccine (lmv) and live parotitis vaccine (lpv) were evaluated in identical groups of children immunized in accordance with different schedules: only lmv or lpv, the injection of these preparations mixed in one syringe or with the use of separate syringes, or at an interval of 3 months. the highest number of febrile reactions, rash and catarrhal symptoms was registered among children immunized with ... | 1986 | 3751401 |
| the effect of arylazido aminopropionyl atp (anapp3) on inhibition of pelvic nerve evoked contractions of the cat urinary bladder. | previously reported work has provided evidence that arylazido aminopropionyl atp (anapp3), a p2-receptor antagonist, blocks the effects of atp and other purine analogs, in the urinary bladder of the cat. this antagonism appeared limited to those effects mediated by p2-receptors, such as contraction of the urinary bladder. however, it was noted that anapp3 did alter the effects of adenosine, mediated through p1-receptors, in some preliminary experiments. a series of experiments was undertaken to ... | 1986 | 3753940 |
| [study of alveolar macrophages stimulated with zymosan in vivo using fluorescent probes]. | zymosan granules injected intravenously (100 mg per kg of body weight) to wistar rats induced strong stimulation of mononuclear phagocyte system. on day 5 zymosan-induced recruitment of monocyte-like cells with high consuming and bactericidal activity into lung spaces could be observed. using fluorescent probes, it was discovered that the size of alveolar cell membranes in zymosan-stimulated or non-stimulated animals was similar, while the viscosity of membrane lipids of alveolar macrophages was ... | 1986 | 3756335 |
| quantitative autoradiographic localization of the dopamine transport complex in the rat brain: use of a highly selective radioligand: [3h]gbr 12935. | | 1986 | 3758162 |
| structures of neorustmicins b, c and d new congeners of rustmicin and neorustmicin a. | | 1986 | 3759645 |
| [acute campylobacter jejuni enterocolitis in children under 2 years of age]. | | 1986 | 3764191 |
| differences in igg1 and igg2 responses of cattle infected with coxiella burnetii and following vaccination. | vaccination of 115 cattle with a formalin-inactivated coxiella burnetii (phase ii) vaccine selectively induced a highly significant igg2 response detectable by enzyme-linked immunosorbent assay. high igg2 and negative or cut off igg1 levels could be detected in uninfected cattle, whereas significant igg1 but lower igg2 levels were associated with c. burnetii infections. | 1986 | 3769444 |
| a proposed model to explain persistent infection of host cells with coxiella burnetii. | l929 mouse fibroblast cells and j774 macrophage-like cells are both susceptible to persistent infection with the q fever agent coxiella burnetti. previously this laboratory has shown that persistently infected cell populations multiply with unaltered generation times or cell cycle progression. it has also been reported by others and us that highly infected cells typically exhibit one large parasite-containing vacuole. we now report that lightly and heavily infected cells are capable of division ... | 1986 | 3772338 |
| coxiella burnetii infection of a vascular prosthesis. | | 1986 | 3773962 |
| endosulfan induced inhibition of 3h-5-hydroxytryptamine uptake in platelets. | endosulfan, a chlorinated pesticide, is widely used to control various insect pests. rats exposed to 1 mg and 3 mg endosulfan/kg for periods of 10, 30, and 60 days showed significant (p less than 0.05) inhibition of [3h]5-hydroxytryptamine (5-ht) uptake by platelet-rich plasma (prp) in an ex vivo study. rats treated with endosulfan (1 and 3 mg/kg) up to 60 days elucidated a marked inhibition of adp-induced aggregatory responses of the platelets. incubation of prp with 10 microm and 100 microm en ... | 1986 | 3775804 |
| comparative study of expression of hemagglutinins, hemolysins, and enterotoxins by clinical and environmental isolates of non-o1 vibrio cholerae in relation to their enteropathogenicity. | a comparative study was undertaken of clinical and environmental isolates of non-o1 vibrio cholerae with respect to their hemagglutinating, hemolytic, enterotoxigenic, and enteropathogenic activities. cell-associated hemagglutinin titers of the clinical and environmental isolates did not differ much, although the clinical isolates displayed higher cell-free hemagglutinin titers compared with those of environmental isolates. culture supernatants of 61.5% (24 of 39) of clinical isolates showed hem ... | 1986 | 3777932 |
| identification of phase-specific antigenic fractions of coxiella burnetti by enzyme-linked immunosorbent assay. | antigenic fractions of coxiella burnetii phase variants were identified with an enzyme-linked immunosorbent assay (elisa). immune sera from guinea pigs immunized with formalin-inactivated phase i or phase ii whole cells were used to measure the antigenic activity of whole cells and various soluble and particulate preparations. phase-specific antigens of c. burnetii whole cells and fractions were compared by dose-response curves at different (antigen and antibody) dilutions. water-soluble extract ... | 1986 | 3782458 |