| turnover of messenger rna in transplantable hepatomas and host liver of rats. | the turnover of messenger rna (mrna) in two intrahepatically transplantable hepatoma (5123 and 19) and host livers of buffalo rats was evaluated with four different approaches. [14c]orotic acid incorporation into the rapidly labeled peak between 18s and 4s of total polyribosomal rna was measured. in vitro rnase assay of [14c]orotic acid-labeled mrna of polyribosomes was utilized. the decay of mrna as reflected by disaggregation of free and membrane-bound polyribosomes at intervals after actinomy ... | 1978 | 206354 |
| circular dichroism and circular polarization of luminescence of reduced nicotinamide adenine dinucleotide in solution and bound to dehydrogenases. | the cd (circular dichroism) and cpl (circular polarization of luminescence) spectra of nadph in aqueous solution were studied and found to be markedly different. the spectra were not affected by cleavage of the coenzyme molecule with phosphodiesterase. the differences are thus not due to the existence of extended and folded conformations of nadph and it is concluded that they originate in excited state conformational changes of the nicotinamide--ribose fragment. opposite signs of both the cd and ... | 1978 | 207311 |
| long-term cultivation of plasma cell leukemia cells and autologous lymphoblasts (lcl) in vitro: a comparative study. | two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (lcl). the 9-month-old plasma cell line showed the typical morphology of plasmoblasts. the cells neither had b- nor t-lymphocyte characterisitics, were ebv negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. the cytogenetic findings indicate a monoclon ... | 1978 | 207377 |
| isolation and characterization of a cytosol protein (64/7.2) present in large amounts in rapidly growing hepatomas. | protein 64/7.2 (molecular weight/isoelectric point) is present in the cytosol of several hepatomas including the novikoff hepatoma and morris hepatomas 9618a, 7794a, and 3924a, but it is not present in liver or 18-hr regenerating liver. quantitatively, its concentration was highest in novikoff hepatoma (150 microgram/g tissue) and morris hepatoma 3924a (550 microgram/g tissue), which are rapid-growing tumors, less in morris hepatoma 7794a (72 microgram/g tissue), which is of intermediate growth ... | 1978 | 207417 |
| second order kinetics of the reduction of cytochrome c2 by the ubiquinone cytochrome b-c2 oxidoreductase of rhodopseudomonas sphaeroides. | | 1978 | 207691 |
| effect of dietary cyclopropene fatty acids on the octadecenoates of individual lipid classes of rat liver and hepatoma. | the effect of dietary cyclopropene fatty acids on the concentration of octadecenoate chain positional isomers in individual lipid classes of normal liver, host liver, and hepatoma 7288ctc has been determined. the data revealed the following: (a) saturated and monoene fatty acid percentages of liver phosphatidylcholines and phosphatidylethanolamines were not affected, but the percentage of saturated fatty acids of the triglycerides and cholesteryl esters was increased while the monoene percentage ... | 1978 | 207942 |
| oral hydration rotavirus diarrhoea: a double blind comparison of sucrose with glucose electrolyte solution. | of 57 male children, aged 5 months to 2 1/2 years with rotavirus diarrhoea, 28 were given oral therapy with sucrose electrolyte solution and 29 were given glucose electrolyte solution in a randomised double-blind trial. all were rehydrated and remained so on oral therapy alone. these patients were compared with 44 children, also with rotavirus, who were treated only with intravenous hydration. the oral therapy and intravenous therapy groups did not differ clinically in the rate of rehydration or ... | 1978 | 209263 |
| operation of the glucose-fatty acid cycle after glycogenolysis induced by treatment with nicotinic acid. | | 1978 | 209743 |
| transfer of polymyxin resistance among enterobacteriaceae and vibrio cholerae. | | 1978 | 210123 |
| pituitary pigmentary hormones. relationship of melanocyte-stimulating hormone to lipotropic hormone. | pituitary control of pigmentation has known for more than 60 years. since 1969, beta-melanocyte-stimulating hormone (beta-msh) has been accepted as the main pituitary pigmentary hormone in man. its "constant companionship" with adrenocorticotrophic hormone (acth) has also been repeatedly demonstrated. current investigations challenge both of these concepts. human beta-msh immunoreactivity has been shown to be actually due to beta-lipotropic hormone (beta-lph), a larger molecule that within itsel ... | 1978 | 210301 |
| kinetic studies on oxidized and partially reduced cytochrome c oxidase. | 1. kinetic studies have been performed with beef-heart cytochrome c oxidase, with the enzyme either in its oxidized, resting state or pretreated anaerobically with different amounts of reduced cytochrome c. the techniques used for the study have been stopped-flow spectrophotometry and electron paramagnetic resonance (epr) spectroscopy. 2. the results show that the one-electron equivalent-reduced enzyme rapidly oxidizes one further equivalent of aerobically or anaerobically added ferrocytochrome ... | 1978 | 210804 |
| the origin of acth cells in rat anterior pituitary. | | 1977 | 211898 |
| [cytopathogenic activity of extracts e. histolytica trophozoites]. | previous studies have not shown any correlation between enzyme activities and pathogenicity of entamoeba histolytica. the activities analyzed were selected without having proved their involvement in the cytopathogenicity. we are using the reverse approach to study the cytopathogenic factors, i.e., to detect and characterize first the cytopathic effect of amebic extracts on animal cells, and subsequently to try to identify the components involved and to explore their correlation with amebic virul ... | 1978 | 211946 |
| comparison of interferon action in interferon resistant and sensitive l1210 cells. | translation inhibition, leu-trna aminoacylation and double-stranded rna and atp dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse l 1210 r and l 1210 s cells. no differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67k dalton phosphoprotein fraction in interferon-treated l 1210 s cell-free extracts. in non-responding cell-free lysates, the lack of sti ... | 1978 | 212514 |
| involvement of brain cyclic amp in the acute and chronic effects of morphine in the rat. | incubation of cerebral cortical slices of rat brain with 3h-adenosine in the presence of varying concentrations of morphine in vitro resulted in a dose-related increase in 3h-camp formation. in in vivo experiments, the rate of 3h-camp formation in cortical slices both 45 min and 4 h after the acute s.c. administration of a 10 mg/kg dose of morphine was significantly greater than that for saline-treated controls. a significant enhancement of cortical 3h-camp formation likewise became apparent 72 ... | 1978 | 212767 |
| evaluation of continuous labelling data by a practical mathematical method with application to a virus-induced sarcoma of the rat. | a simple mathematical function is established which renders the increase of the labelling index during continuous labelling of an exponentially growing tumor. by known values of proliferative fraction, mean cell cycle time and tumor doubling time, the mode and extent of cell loss is determined. three models for different modes of cell loss are introduced: model a for cell loss equally affecting proliferative and resting cells; model b for cell loss predominantly affecting one of the two cell com ... | 1978 | 212886 |
| [several features of the metabolism of the fast and slow muscles of emys orbicularis tortoises]. | in skeletal muscles of the tortoise e. orbicularis, studies have been made on the content of glycogen, lactic acid, on the activity of glucose-6-phosphatase and phosphorylase. histochemical studies were made on the lipid content. experiments were performed on fast and slow bundles from mm. iliofibularis, testo cervicalis and retractor capitis. for comparison, the same indices of carbohydrate metabolism were investigated in fast m. plantaris and slow m. soleus of rats. in rats, slow muscles exhib ... | 1978 | 213913 |
| inhibition by propranolol of bile acid- and pge1-stimulated camp and intestinal secretion. | three colonic and three ileal loops were prepared in six rabbits pretreated with propranolol (pr) 4 mg./kg. i.v. and in five untreated rabbits. in random order, 1 ml. of either deoxycholic acid (dca) 6 mm., prostaglandin e1 (pge1) 20 microgram./ml., or saline was placed in each colonic loop and 1 ml. of either cholera enterotoxin (ce) 10 microgram./ml., pge1 20 microgram./ml., or saline was placed in each ileal loop. in untreated animals, dca and pge1 in the colon and ce and pge1 in the ileum st ... | 1978 | 213967 |
| thyroid hormone action at the cell level (first of two parts). | | 1979 | 214707 |
| parainfluenza-3 virus: difference in capacity of neuraminidase-weak and strong strains to infect young calves and to elicit cellular immune response. | calves less than four weeks old could not be infected with a neuraminidase-weak strain of parainfluenza-3 virus (pi3) but were successfully infected with either of two neuraminidase-strong strains. the criteria for infection were virus excretion, cell-mediated and antibody-mediated immune responses. in the lymphocyte stimulation test, calves infected with the neuraminidase-strong pi3 strain tüb-e6 responded more strongly to antigen prepared from this strain than to antigen from the heterologous ... | 1978 | 214834 |
| intracellular metabolism of sendai virus nucleocapside. | | 1978 | 214959 |
| structural specificity of the nak-atpase inhibition by sanguinarine, an isoquinoline benzophenanthridien alkaloid. | | 1978 | 215160 |
| elisa: enzyme-linked immunosorbent assay. | similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. the end point is a color change that can be assessed by colorimetry or with the naked eye. various techniques of elisa are described, along with examples of current and potential clinical applications. | 1978 | 215505 |
| detection of mouse mammary tumor virus rna in balb/c tumor cell lines of nonviral etiologies. | a complementary dna (cdna) probe to mouse mammary tumor virus (mmtv) rna was synthesized using calf thymus dna oligonucleotides as a random primer. this probe was then used to study the expression of mmtv rna in cell lines from balb/c tumors induced in vivo either spontaneously or in response to viral, chemical, or hormonal stimuli. the cdna had a length of approximately 400 to 500 nucleotides and specifically hybridized to mmtv rna and balb/c lactating mammary gland rna, but not to moloney leuk ... | 1978 | 215778 |
| codistribution of pericellular matrix proteins in cultured fibroblasts and loss in transformation: fibronectin and procollagen. | antibodies to fibronectin and to distinct types of procollagens and collagens were used in immunofluorescent staining to localize these proteins in cell cultures. normal human skin or lung fibroblasts produced a fibrillar pericellular matrix in which fibronectin and procollagen (types i and iii) showed extensive codistribution. fibronectin and procollagen were synthesized by the same cells as judged by double-stain immunofluorescence. pericellular procollagen was specifically digested with colla ... | 1978 | 217006 |
| [the surface structure of human serum lipoproteins]. | | 1978 | 217189 |
| effects of exogenous prostaglandin e1 on human plasma norepinephrine level and dopamine-beta-hydrolyxase activity. | effects of exogenous pg e1 on the level of human plasma norepinephrine (ne), dopamine-beta-hydroxylase (dbh) activity, camp, cgmp, free fatty acids (ffa) as well as pulse rate and blood pressure were studied. significant decreases of blood pressure and increases of pulse rate were observed after 20 min of the infusion of 0.05 microgram/kg/min of pg e1. the level of plasma ne increased by 174% (p less than 0.005) after the infusion. however, the increase of dbh activity was not significant. there ... | 1978 | 217212 |
| specificity of action of neuraminidase, according to its bacteriological origin. | neuraminidase from vibrio cholerae selectively cleaves sialic acid from fsh, but leaves the lh sialic acid, not influencing the biological activity of the latter hormone. on the other hand, neuraminidase from clostridium perfringens does not possess this specific action and destroys the biological activity of lh as is suggested by parlow's oaad-test. | 1978 | 217679 |
| in vivo suppression of allograft rejection by cyclic amp increasing agents. | this study was undertaken to elucidate the possibilities of modulating the survival of allogeneic tissue grafts by administration of cyclic amp-increasing agents to the graft recipients. heterotopic grafting of split mouse hearts across a strong h-2 barrier was used. it was found that vibrio cholerae enterotoxin, which activates adenylate cyclase in mammalian cells, gave a marked prolongation of the graft survival time when given shortly before (but not after) transplantation. also the administr ... | 1979 | 217841 |
| effects of prostaglandins on excitatory transmission in isolated canine tracheal muscle. | | 1978 | 218032 |
| effect of osteotropic hormones on cartilage metabolism. | | 1979 | 218877 |
| modulation of hexose uptake and insulin action by cell membrane fluidity. the effects of temperature on membrane fluidity, insulin action, and insulin binding. | | 1979 | 218948 |
| enhancement in ia-motoneuron synaptic transmission caudal to chronic spinal cord transection. | | 1979 | 219161 |
| 3-substituted pyrazole derivatives as inhibitors and inactivators of liver alcohol dehydrogenase. | 3-substituted pyrazoles, hoch2 (1), hoch2ch2 (2), hoch2ch2ch2 (3), clch2 (4), clch2ch2 (5), clch2ch2ch2 (6), and ch3co (7), were synthesized and evaluated in vitro on horse liver alcohol dehydrogenase for their potential as inhibitors of ethanol metabolism. 1 to 6 bound to the enzyme-nad+ complex with dissociation constants of 40 to 200 microm, much higher than the constants for the corresponding 4-substituted pyrazoles, but with the same absorption maximum at 295 nm. 4 inactivated the enzyme wi ... | 1979 | 219196 |
| nucleosomal structure of epstein-barr virus dna in transformed cell lines. | micrococcal nuclease digestion was used to analyze epstein-barr virus (ebv) dna structure in nuclei of transformed cells. digests of virus-producing (p3hr-1), non-virus-producing (raji), and superinfected rajii cell nuclei were fractionated by electrophoresis on agarose gels, transferred to nitrocellulose, and hybridized to 32p-labeled ebv dna. the viral dna of raji nuclei produced a series of bands on electrophoresis whose lengths were integral multiples of a unit size, which was the same as th ... | 1979 | 219253 |
| herpes simplex virus infections. | | 1979 | 219403 |
| cytomegalovirus infection in cancer patients. | | 1978 | 219406 |
| effect on platelet activity of inhibition of adenylate cyclase. | | 1978 | 219558 |
| prevalence of antibodies to equine viruses in the netherlands. | the prevalence of antibodies to various viruses was investigated in a series of serum samples collected from horses in the netherlands between 1963 and 1966 and from 1972 onwards. neutralizing antibodies to equine rhinopneumonitis virus, equine arteritis virus and to equine rhinovirus types 1 and 2 were detected in respectively 76%, 14%, 66% and 59% of the equine serum samples tested. the observed incidence of serum samples positive to equine adenovirus in the complement fixation test was 39%. p ... | 1979 | 219560 |
| occurrence of hbsag, anti-hbs, the anti-cmv following the transfusion of blood products. | a retrospective study of the serum of 104 patients treated at chelsea naval hospital between 1969 and 1972 was done. the donor blood products were not tested for the hepatitis b antigen before transfusion. the incidence of hepatitis b antigen following transfusion was about 2.8 per cent. the incidence of antibody to hbsag prior to transfusion was 16 per cent, and about 27 per cent of the patients developed antibody to hbsag following transfusion. the incidence of antibody to cytomegalovirus was ... | 1979 | 219565 |
| serum antibodies to some oncogenic viruses in the child. | the presence of complement fixing antibodies to four viruses with oncogenic potential for animals was studied in children (including neonates and infants). antibodies to herpes, adenovirus and avian "gs" sarcoma-leukosis antigens were detected, but there were no antibodies to sv-40. positivity percentages varied with age and with the antigen used. possible explanations of the appearance of these antibodies in the child are provided. | 1978 | 219589 |
| thyroglobulin interactions with thyroid plasma membranes. the existence of specific receptors and their potential role. | thyroglobulin binds to isolated thyroid plasma membrane preparations. binding is ph- and temperature-dependent with 10-fold better binding at ph 5.0 and 37 degrees c than at 0 degrees c and ph 6.0 through ph 7.5. binding is, however, maximal in 90 min at all ph values and temperatures examined. although salts can inhibit or enhance thyroglobulin binding depending on the temperature or ph, conditions approaching those of the physiological state are not inhibitory; physiological conditions do inhi ... | 1979 | 221458 |
| enzymic activity of cholera toxin. ii. relationships to proteolytic processing, disulfide bond reduction, and subunit composition. | cholera toxin containing intact a chain (mr = 29,000) was isolated, and its enzymic properties were characterized. the "unnicked" form of the toxin, produced by a protease-deficient, hypertoxinogenic mutant of vibrio cholerae 569b, had greatly reduced activity in catalyzing the nad+-glycohydrolase and adp-ribosyltransferase reactions as compared to the naturally nicked form commonly isolated. in the latter, the intact a chain has been cleaved by bacterial proteases to yield disulfide-linked a1 a ... | 1979 | 221485 |
| nad-dependent adp-ribosylation of arginine and proteins by escherichia coli heat-labile enterotoxin. | escherichia coli heat-labile enterotoxin (labile toxin, lt) catalyzed the hydrolysis of nad to adp-ribose and nicotinamide and the adp-ribosylation of arginine (moss, j., and richardson, s.h. (1978) j. clin. invest. 62, 281-285). analysis of the product of the adp-ribosylation of arginine by nuclear magnetic resonance spectroscopy indicated that the reaction was stereospecific and resulted in the formation of alpha-adp-ribosyl-l-arginine. this reaction product rapidly anomerized to yield a mixtu ... | 1979 | 221495 |
| secretory granules of the duodenal enterochromaffin cells of the cat. | | 1979 | 222959 |
| shigella infection of henle intestinal epithelial cells: role of the host cell. | the process of henle 407 embryonic intestinal epithelial cell infection by shigella flexneri 2a m42-43 was studied in an in vitro model system. the role of the henle cell was assessed. it was established that entry of s. flexneri into cells was suppressed by reagents which inhibit uptake of particles by phagocytic cells. the compounds tested included cytochalasin b, dibutyryl-cyclic adenosine monophosphate, choleragen (vibrio cholera enterotoxin), iodoacetate, and dinitrophenol. cytochalasin b i ... | 1979 | 223988 |
| characterization of membrane-bound nicotinamide adenine dinucleotide glycohydrolase of vibrio cholerae. | | 1979 | 225325 |
| reversible binding of vibrio cholerae toxin on nad-sepharose. | when a crude or partially purified culture of vibrio cholerae is passed through a column of nad-sepharose, the skin toxic material is bound to the gel. choleragen may be recovered by increasing the ionic strength of the elution buffer, as shown by the skin test, immunodiffusion against anti-choleragen antiserum and polyacrylamide gel electrophoresis. affinity chromatography on nad-sepharose may be helpful for the concentration and purification of choleragen. | 1978 | 226497 |
| neuraminidase inhibition by chemically sulphated glycopeptides. | chemically sulphated glycopeptides (derived from pig duodenal mucosa) inhibited clostridium perfringens neuraminidase (ec 3.2.1.18) activity in a ph-dependent manner. analysis of inhibition kinetics data indicated that, although the enzyme inhibition could not be categorized into any of the classical types of inhibition, it could be interpreted as a function of the size and shape of the substrates used. the enzyme activity was inhibited by 86% and 40% when tested with bovine submaxillary-gland m ... | 1979 | 227363 |
| [effect of vibrio cholerae filtrate on the adenyl cyclase system in vitro]. | an in vitro model using homogenates of the rat intestine and liver for studying the v. cholerae culture filtrate effect on the adenylate cyclase system is proposed. optimal conditions for the adenylate cyclase functioning have been investigated for this model. it was shown that v. cholerae filtrate induces a stable activation of adenylate cyclase and does not change the activity of cyclic amp phosphodiesterase. it was also established that the activity of phosphodiesterase in the intestinal homo ... | 1979 | 227494 |
| identification of the cyclic amp-dependent protein kinase responsible for endogenous phosphorylation of substrate proteins in synaptic membrane fraction from rat brain. | | 1979 | 227891 |
| cyclic adenosine monophosphate response to prostaglandin e2 on subpopulations of human lymphocytes. | receptors for prostaglandin e2 or histamine were measured on subpopulations of human lymphocytes, using the cyclic amp increase after exposure to prostaglandin or histamine as an indicator for the presence of receptors. the cyclic amp response to prostaglandin e2 was similar in unfractionated lymphocytes and the t-enriched and t-depleted fractions. within the t-enriched population, t cells bearing a receptor for the fc portion of igg (t gamma-cells) had a 27.4-fold rise in cyclic amp after expos ... | 1979 | 227981 |
| escherichia coli heat-labile enterotoxin: dna-directed in vitro synthesis and structure. | escherichia coli heat-labile enterotoxin was synthesized in a cell-free system directed by dna of the plasmid p307. synthesis of the toxin, assayed by the elongation induced in chinese hamster ovary cells, was strongly stimulated by cyclic amp and occurred at physiological levels of mg2+ only when the polyamine spermidine was present. activity was abolished by heat and antisera prepared against the enterotoxins of both e. coli p263 and vibrio cholera. tritium-labeled enterotoxin was purified by ... | 1979 | 228267 |
| [atypical immunoproliferative processes. proposed nosographic classification, clinico-pathological forms, pathogenetic theories and relations with malignant lymphomas]. | | 1979 | 228362 |
| isolation of foot-and-mouth disease virus messenger rna from membrane-bound polyribosomes and characterization of its 5' and 3' termini. | | 1979 | 228483 |
| serological markers for hepatitis types a and b among u.s. arym soldiers, germany. | viral hepatitis rates among u.s. army soldiers in europe have been found to be two to three times higher than corresponding rates for soldiers stationed in the u.s. sera from 89 per cent of a representative army unit with 865 members and a known hepatitis problem were tested for hbsag, anti-hbs, anti-hbc, and anti-ha. the prevalence of hb markers was 20 per cent, and hepatitis a antibody was present in 25 per cent. a six-month follow-up, conducted on 260 individuals initially negative for all fo ... | 1979 | 228562 |
| camp inhibits the in vitro assembly of microtubules. | | 1979 | 228610 |
| protein phosphorylation in human peripheral blood lymphocytes. subcellular distribution and partial characterization of adenosine 3':5'-cyclic monophosphate-dependent protein kinase. | cytoplasmic and membrane fractions prepared from human peripheral-blood lymphocytes both contained cyclic amp-dependent protein kinase activity and endogenous protein kinase substrates. protein kinase activity in the particulate fractions was not eluted with 0.25 m-nacl, suggesting that it was not derived from non-specifically absorbed soluble cytoplasmic protein kinase. nor was the particulate protein kinase activity eluted by treatment with cyclic amp, suggesting that the catalytic subunit is ... | 1979 | 228656 |
| regulation of the in vitro anamnestic antibody response by cyclic amp. iii. cholera enterotoxin induces lymph node cells to release soluble factor(s) which enhance(s) antibody synthesis by antigen-treated lymph node cells. | | 1979 | 228867 |
| desialized low-density lipoprotein regulates cholesterol metabolism in receptor-deficient fibroblasts. | | 1979 | 228933 |
| effects of several preoperative medications on fat cell lipolysis, and activity of adipose tissue cyclic amp phosphodiesterase. | effects were examined of atropine, diazepam, pethidene, promethazine, scopolamine, omnopon and papaverine on basal and noradrenaline-stimulated lipolysis in rat isolated fat cells and on rat adipose tissue cyclic amp phosphodiesterase activity. papaverine at high concentration (1 mm) inhibited both basal and hormone-stimulated lipolysis, whereas diazepam enhanced basal lipolysis. at a 'clinical dose', omnopon increased both basal and noradrenaline-stimulated lipolysis. adipose tissue camp phosph ... | 1979 | 228965 |
| factors involved in skin carcinogenesis. | a personal account of skin carcinogenesis is given based on forty years of investigation of normal and pathologic anatomy of the human skin. symbiosis of acral portions of adnexa and of melanocytes with epidermal keratinocytes is stressed, and perversion of symbiosis to parasitism in tumor formation is discussed. the role of the basement membrane as a combined effort of, and filter between, ectoderm and mesoderm is stressed, and the nature of basal cell epithelioma as the least differentiated me ... | 1979 | 229133 |
| autoradiographic visualization of beta-adrenergic receptors in normal and denervated skeletal muscle. | autoradiographic localization of beta-adrenergic receptors in rat skeletal muscle in vivo was achieved utilizing [125i]-iodohydroxybenzylpindolol, a potent beta-adrenergic blocker with high affinity and specificity for those receptors. in normal muscle the beta-adrenergic receptors were localized mainly to blood vessels, arterioles greater than venules, with much less concentration of grains over the fascicles of muscle fibers. one week after denervation there was an increase in binding both to ... | 1979 | 229156 |
| effect of lipid extraction, adrenalectomy, and cortisol upon free radical formation by rat lung microsomes. | | 1979 | 229351 |
| [heterosynaptic interactions in fields ca1 and ca3 of the hippocampus in vitro]. | heterosynaptic interactions between the perforant path and schaffer's collaterals in the field ca1 and between the perforant path and dentate mossy fibres in the field ca3 were investigated in guinea pig hippocampal slices. using the method of paired stimuli, space-time summation (for 20--50 ms) was observed in both systems with stimuli sub-threshold for spike generation. spike responses of the neurons to testing stimulation of afferents synapsing upon the terminal parts of apical dendrites (per ... | 1979 | 229431 |
| immunoreactivity for beta-endorphin in lh-rh neurons of the fetal human hypothalamus. | a peptide immunochemically related to beta-endorphin was detected in some lh-rh neurons of the fetal human hypothalamus by comparison of adjacent sections stained for beta-endorphin and for lh-rh. in the same section, by successive staining and after antibody elution, both peptides were again revealed in the same neuron. the significance of the presence of the beta-endorphin-like material in lh-rh neurons is discussed. | 1979 | 229958 |
| [site specific mutagenesis (author's transl)]. | | 1979 | 230015 |
| the presence and cleavage of interpeptide disulfide bonds in viral glycoproteins. | | 1979 | 230184 |
| effects of cytoskeletal perturbant drugs on ecto 5'-nucleotidase, a concanavalin a receptor. | differences in cell morphology, concanavalin a-induced receptor redistributions, and the cooperativity of the inhibition of 5'-nucleotidase (ampase) by concanavalin a (con a) have been investigated in ascites sublines of the 13762 rat mammary adenocarcinoma cells treated with microfilament- and microtubule-perturbing drugs. by scanning electron microscopy mat-c1 cells exhibit a highly irregular surface, covered with microvilli extending as branched structures from the cell body. mat-a, mat-b, an ... | 1979 | 230191 |
| [occurrence of nag vibrios in berlin waters]. | | 1979 | 232335 |
| dynamic and static components in the myogenic control of vascular tone in cat skeletal muscle. | | 1979 | 232614 |
| high-affinity binding processes for gaba, glycine, and beta-alanine in synaptosome-enriched fractions of rats cns. | a bicuculline-sensitive component of the binding of gaba and strychnine-sensitive components of the binding of glycine and beta alanine to synaptosome-enriched fractions of rat cns have been demonstrated in the presence of physiological concentrations of na+ and other inorganic ions. these processes might be related to synaptic receptors for these inhibitory amino-acids. | 1979 | 232720 |
| [hygienic status of small streams on the periphery of rome. iii. research on pathogenic microorganisms]. | | 1979 | 233380 |
| therapy in an intracerebral murine glioma model, using bacillus calmette-guérin, neuraminidase-treated tumor cells, and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea. | the s.c.-propagated murine glioma, gl-26, was established in tissue culture. the tissue culture line, with a doubling time of 36 hr, was used as the common source for all tumor cells. suspensions of the tumor cells were transplanted intracerebrally in mice to produce an anaplastic ependymoblastoma. in vitro 51-cr cytotoxicity assays did not detect any cellular immunity against gl-26 tumor cells in animals bearing either s.c. or i.c. tumors, indicating that the tumor itself is not highly immunoge ... | 1975 | 234790 |
| microbial interference with host defence mechanisms. | | 1975 | 235083 |
| natural antibodies in healthy adults. | the serum of 100 adults living in budapest was examined for isohaemagglutinin titre with haemaglutination, for staphylococcal-antitoxin titre with haemolysis inhibition and for bacterial antibody titre against 17 different groups of bacteria with passive haemagglutination. antibody levels in males, except for certain bacterial antibodies, were somewhat lower than in females. the antibody titres, especially in men, decreased gradually from 20 to 50 years of age and were usually lower in rh negati ... | 1975 | 235827 |
| resolution, purification and characterization of the orthophosphate releasing activities from fracture callus calcifying cartilage. | callus calcifying cartilage alkaline phosphatase was resolved by deae-cellulose column chromatography into two distinct phsophatase activities. the phosphatase activity which was eluted first from the column, (phosphatase i), was active towards a variety of phosphate esters, sodium pyrophosphatase and several linear polyphosphates, while the second phosphatase activity , (phosphatase ii), was active toward simple phosphate esters but not towards sodium pyrophosphate and linear oligo or polyphosp ... | 1975 | 238599 |
| purification and characterization of two activities of the intracellular dextransucrase from leuconostoc mesenteroides nrrl b-1299. | dextransucrase (sucrose: 1,6-alpha-d-glucan 6-alpha-glucosyltransferase ec 2.4.1.5) activity of leuconostoc mesenteroides nrrl b-1299 cells has been purified by adsorption on hydroxyapatite, followed by chromatographies on deae-cellulose and deae-sephadex. the enzyme activity was readily separated into two principal forms of the enzyme, i and ii, by deae-cellulose chromatography. both enzymes i and ii were purified to an electrophoretically homogeneous state in which the relative enzyme activiti ... | 1975 | 238637 |
| intestinal villus and crypt cell responses to cholera toxin. | adenylate cyclase activity was measured in rat small intestinal villus and crypt cells after in vivo and in vitro exposure to cholera toxin. the increase in intestinal adenylate cyclase induced by cholera toxin in vivo appeared to be largely confined to the villus cell with the largest increase observed for upper villus cells. crypt cell adenylate cyclase was not responsive to cholera toxin. no response could be demonstrated for isolated villus or crypt cells incubated with cholera toxin in vitr ... | 1975 | 238900 |
| methods for analyzing percentage lipid of ground beef and beef-soy blends. | | 1975 | 239047 |
| factors influencing the excretion and relative physiological availability of pethidine in man. | factors influencing the urinary excretion of pethidine, norpethidine and pethidine n-oxide have been examined. the proportion of a dose of pethidine excreted unchanged or as norpethidine depends on the urinary ph and the route of administration. older people appear to metabolize more of the drug and therefore excrete less unchanged pethidine. the rate of excretion of pethidine in acid urine is directly proportional to the plasma pethidine concentration and under these conditions the relative phy ... | 1975 | 239115 |
| the role of dopamine and noradrenaline in temperature control of normal and reserpine-pretreated mice. | drugs with the common property of stimulating dopamine receptors, have been tested for their effects on core temperature in control and reserpine-pretreated mice. apomorphine, amantadine, amphetamine, l-dopa and atropine all produced a fall in mouse oesophageal temperature, their efficacy correlating with their ability to activate central dopamine receptors. amphetamine and l-dopa had a biphasic effect the initial fall being followed by a rise. in reserpine-pretreated mice only amphetamine, apom ... | 1975 | 239116 |
| cholinergic modification of abnormal involuntary movements induced in the guinea-pig by intrastriatal dopamine. | | 1975 | 239121 |
| the alpha-adrenoceptor blocking effects of a new benzodioxane. | | 1975 | 239130 |
| [clinical effects of lividomycin on respiratory tract infections, primarily wet cases of bronchiectasis (author's transl)]. | patients with various respiratory infections, primarily wet cases of bronchiectasis, were treated with lividomycin (lvdm) at a dose of 1 g/day for about a week. the results obtained are as follows: 1. of the 15 patients with refractory bronchiectasis who had previously been treated with various other antibiotics, 11 patients (73.3%) responded to lvdm. although there was no patient who responded to lvdm excellently, good cures were achieved in 5 pateints and fair cures in 6 patients. 2. in bronc ... | 1975 | 239263 |
| glutathione conjugates as metabolites of benz[a]anthracene. | 1. [3h]benz[a]anthracene is converted into water-soluble metabolites by microsomal plus soluble fractions of rat-liver in the presence of nadph and glutathione. chromatography on sephadex g25 gave four radioactive peaks; the first contained hydrocarbon or hydrocarbon derivatives bound to soluble protein while the other three peaks contained glutathione conjugates of hydrocarbon metabolites. 2. conjugates formed when either of the benz[a]anthracene metabolites, 5,6-dihydro-5,6-dihydroxybenz[a]ant ... | 1975 | 239491 |
| boric acid tolerant vibrio cholerae: biological and physical properties. | two boric acid tolerant variants were developed by passage in boric acid containing media from a mouse virulent parent vibrio cholerae strain. the variants were stable, exhibited increased acid resistance, were less virulent to mice, but protected against challenge with the mouse virulent parent strain. | 1975 | 239521 |
| new concepts in meat processing. | | 1975 | 239547 |
| properties of the cholera phage pl 163/10. | vibrio cholerae phage pl 163/10, belonging to mukherjee's group i, gave clear plaques with surrounding halos of overall diameters varying between 1 to 4 mm when plated on a lawn of host v. cholerae ogawa 154. it was fairly stable in the ph range 6-11. its thermal inactivation was characterised by half lives of 39, 12, 4.5 and 1.0 minutes at 55, 60, 65 and 70 degree c respectively. the thermodynamic parameters deltah, deltaf and deltas were determined at these temperatures. the phange was resista ... | 1975 | 239574 |
| the "otitis-prone" condition. | we studied the incidence of exudative otitis media in 488 patients followed up from birth. forty-nine percent (240) of the patients had their initial episode of otitis media in the first year of life and only 12% (56) in the second year of life. thereafter, the incidence decreased steadily. patients having six or more episodes of otitis media before age 6 were termed "otitis prone". fifty-seven such patients were observed and this condition was found to be significantly related to the onset of o ... | 1975 | 239591 |
| method for detecting small numbers of vibrio cholerae in very polluted substrates. | a method is presented for the indirect detection of vibrio cholerae by the multiplication of two specific bacteriophages: phih74/64 for el-tor vibrios, and phage group iv (mukerjee) for classical vibrios. the product to be examined is seeded in alkaline tryptone water for enrichment, as in the classical method, and is then incubated for 6 h at 37 c. thereafter, a loopful is transferred to each of two nutrient broth (ph 9) tubes. one of these receives a drop of phage phih74/64; the other receives ... | 1975 | 239631 |
| [characteristics of a mycobacterium mucosum culture oxidizing cholic acid]. | some physiologo-morphological and biochemical characteristics were studied with the culture of mycobacterium mucosum 1210 which can assimilate cholic acid as a sole source of carbon. this culture is capable of spontaneous variability. four variants of the culture were obtained which possessed different rates of the transformation of cholic acid. the variant which formed slimy colonies of pale-yellow colour was the most active. some morphological modifications of the cells were investigated in th ... | 1975 | 240106 |
| [michaelis constants of neuraminidases of pathogenic and apathogenic microorganisms (author's transl)]. | the km-values of neuraminidases from different pathogenic and apathogenic microorganisms have been determined on low and high molecular substrates. the substrate specificity and the affinity to the different types of substrates in relation to the pathogenicity of the microorganisms are discussed. | 1975 | 241169 |
| aryl hydrocarbon hydroxylase in liver nuclei of c3h/he and dba/2 mice. | aryl hydrocarbon hydroxylase activity in liver nuclei from c3h/he mice is apparently increased by the administration of 3-methylcholanthrene, but the enzyme activity from the dba/2 mice is not. on the other hand, by treatment of the mice with phenobarbital sodium, the increased activity in liver nuclei was observed in both strains of mice. there are approximately the same levels of the apparent km for benzo[alpha]pyrene in liver nuclei from both strains of mice even after treatment with 3-methyl ... | 1975 | 241681 |
| assay and properties of glutathione-s-benzo(a)pyrene-4,5-oxide transferase. | | 1975 | 242265 |
| [ergonomy and nursing care: how to chose adequate equipment]. | | 1977 | 242442 |
| vibrio cholerae hybrid sex factor that contains ampicillin transposon tn1. | the ampicillin resistance transposon tn1 was translocated from the r plasmid rp4 to the vibrio cholerae conjugative plasmid, p. the hybrid sex factor p::tn1 was highly transmissible and expressed the biological activities of the p factor. in addition, p::tn1 facilitated transfer of rp4 to v. cholerae recipients. physical studies of p::tn1 indicated that the tn1 transposon was added to the otherwise unaltered p plasmid. | 1979 | 253004 |
| [human science. awareness (2). awareness and psychophysiological problems]. | | 1979 | 259791 |
| intestinal parasitism of bulls on growth performance tests in arkansas. | | 1979 | 260548 |
| use of oral vaccines in attempts to prevent swine dysentery. | | 1979 | 260870 |