| [a dry culture medium for determining the cellular composition of strains of the plague agent based on its requirements for calcium ions]. | | 1988 | 2463423 |
| molecular evolution of the human and simian immunodeficiency viruses. | molecular evolution and phylogeny of different human immunodeficiency virus type 1 (hiv1) strains, of a type 2 (hiv2) strain, and of two simian immunodeficiency viruses (sivagm and sivmac) have been studied by comparing the nucleotide sequences of the two regions of their pol genes which encode the reverse transcriptase (rt) and endonuclease/integrase (en). the analyses show that the different hiv 1s form one cluster (hiv1 group) and that the sivs and hiv2 form another (hiv2 group). when the ent ... | 1988 | 2464734 |
| [quantitative determination of specific plague fluorescent immunoglobulin activity using pulse flow cytofluorometry]. | | 1988 | 2466163 |
| the gene coding for the 190,000-dalton iron-regulated protein of yersinia species is present only in the highly pathogenic strains. | a genomic library containing dna fragments of 0.5 to 2 kilobase pairs in length from yersinia enterocolitica serovar o:8 was constructed in a bacteriophage lambda gt11 expression vector. mouse antibodies specific for the iron-regulated high-molecular-weight proteins (hmwps) were used to screen the library. two positive clones of 1 and 0.5 kilobase pairs, designated a13 and d7, respectively, were detected and isolated. they coded for beta-galactosidase fusion proteins of 151,000 and 138,000 dalto ... | 1989 | 2466794 |
| [the agar precipitation reaction as a method of selecting fraction-free strains of yersinia pestis]. | | 1989 | 2468040 |
| [a method of fixation and disinfection of microbial cells on magnetic sorbents]. | the 96% ethanol has been found fairly effective for the fixation and disinfection of yersinia pestis, vibrio cholerae, pseudomonas mallei, and pseudomonas pseudomallei after their contact with magnetic polyacrylamide sorbents containing specific immunoglobulins for ligands. such step is recommended for the quantitative immunofluorescence test employed for rapid analysis, diagnosis, and identification of the above microorganisms. | 1989 | 2474712 |
| [methodology of determining yersinia pestis antigens using dot-immunologic analysis]. | the technique of dot-immunoassay on nitrocellulose filters is described for the detection of the vital infection (vi) agents. the results are read visually (qualitatively) and instrumentally (quantitatively). the method is highly sensitive (3.2 +/- 0.8 ng/ml of fraction i) and specific, cost-effective, simple, and available for laboratories engaged in vi diagnosis. | 1989 | 2474713 |
| selective radioiodination of the apical and luminal cell surfaces: in vitro and in situ experiments on vascular endothelial cells with iodogen-coated sephadex. | a gentle and nonexpensive agent for selective radioiodination of the cell surface proteins was obtained by plating aliquots of iodogen on dried sephadex beads 50-60 microns in diameter. iodogen-coated sephadex inherits iodogen properties: it is stable and virtually insoluble in water, allowing rapid iodination of the cell surface proteins in the solid phase with 125i-. iodination is terminated by simply removing the beads. the agent was tested on bovine aortic endothelial cells in culture and on ... | 1989 | 2476041 |
| [isolation of high-affinity antibodies from hyperimmune serum]. | a group of high-affinity antibodies has been isolated from horse hyperimmune antiplague serum with the use of antigenic immunosorbent. high-affinity antibodies have proved to be homogeneous, which is indicated by the rectilinear character of the graph of the inverse values of the free antigen concentration. | 1989 | 2476900 |
| molecular analysis of lcrgvh, the v antigen operon of yersinia pestis. | the lcrgvh operon of plasmid pcd1 in yersinia pestis kim encodes the virulence-associated v antigen, the regulatory protein lcrh, and lcrg, a protein of undefined function. in this study we sequenced lcrgvh and analyzed it for transcription initiation sites. there were three open reading frames within the sequence, 288, 981, and 507 bases in length, which could encode proteins with molecular weights and isoelectric points corresponding to those of lcrg, lcrv (v antigen), and lcrh, respectively. ... | 1989 | 2477361 |
| differential immunohistochemical localization of cytokeratins and collagen types i and iii in experimentally-induced cirrhosis. | liver cirrhosis was induced in male wistar rats by subcutaneous injection (1 ml of 30 g/l) of an aqueous solution of thioacetamide. using the indirect immunoperoxidase technique, high molecular weight keratins were localized in bile ducts and ductules. low molecular weight cytokeratins were present in regenerating hepatocytes in active cirrhosis; bile ducts were unstained. these results suggest that cytokeratin staining may be useful in distinguishing bile duct epithelium and hepatocytes in hepa ... | 1989 | 2478687 |
| barium- and calcium-permeable channels open at negative membrane potentials in rat ventricular myocytes. | ca2+- and ba2+-permeable channel activity from adult rat ventricular myocytes, spontaneously appeared in the three single-channel recording configurations: cell-attached, and excised inside-out or outside-out membrane patches. single-channel activity was recorded at steady-state applied membrane potentials including the entire range of physiologic values, and displayed no "rundown" in excised patches. this activity occurred in irregular bursts separated by quiescent periods of 5 to 20 min in cel ... | 1989 | 2478710 |
| [immunoelectrophoretic analysis of the antigenic composition of yersinia]. | the antigenic composition of 24. y. pseudotuberculosis newly isolated and reference strains, 7 y. enterocolitica strains, as well as y. pestis vaccine strain ev, has been studied by the method of immunoelectrophoresis in agar. the antigenic composition of these bacteria has been found to be complicated and to comprise not less than 8-11 antigens, and among them nonspecific protein antigens common for enterobacteria, the common generic antigen, the antigen common with y. pestis, as well as o-anti ... | 1989 | 2480038 |
| the kinetics of human granulopoiesis following treatment with granulocyte colony-stimulating factor in vivo. | cell proliferation in the bone marrow and blood of two patients with metastatic breast cancer who were treated with granulocyte colony-stimulating factor was studied by using [3h]thymidine labeling and autoradiography. additionally, the fate of neutrophils labeled with 99mtc-hexamethylpropyleneamineoxime was observed following granulocyte colony-stimulating factor infusion. proliferation increased in all stages of granulopoiesis, but a significant amount of the increased production stemmed from ... | 1989 | 2480603 |
| [control of anticoagulant therapy by using soluble thromboplastin]. | the characteristics of soluble thromboplastin, a new coagulant agent, were under study. this drug is a high-molecular lipoprotein complex of human cadaver brain, purified by gel chromatography. the new preparation has been standardized and compared to thromboplastin preparations manufactured in this country and abroad. soluble thromboplastin has been found fairly sensitive to reductions of the prothrombin factors levels, it may be used for monitoring the efficacy of indirect anticoagulant therap ... | 1989 | 2481064 |
| [natural infection by yersinia pestis, in fleas from plague foci in the northeast of brazil]. | three different containment transport processes of fleas were evaluated as an approach to the bacteriologic isolation of yersinia pestis. the three methods employed were: live fleas in glass tubes containing pieces of wrapped filter paper; dead fleas in saline solution; and macerated fleas in cary-blair culture medium. the two latter methods were almost equal and superior to the first method. a total of 29512 flea pools, from plaque foci in northeast brazil collected during 1966 to 1982 were eva ... | 1989 | 2487774 |
| [evaluation of 2 methods for studying slime production by coagulase-negative staphylococcus strains]. | the surface hydrophobicity and the slime production of 46 coagulase negative staphylococci isolated from the urine, blood and skin were evaluated. the hydrophobicity values were very heterogeneous and did not correlate with the species, origin or slime production. slime production was evaluated by the classical tube method and by a micromethod with spectrophotometric readings. there was a high correlation between both methods, but the results were more reproducible and quantifiable with the latt ... | 1989 | 2490721 |
| lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats. | | 1989 | 2492809 |
| endothelial cells from human umbilical vein secrete functional transcobalamin ii. | transcobalamin ii (tcii) is a cobalamin (cbl) binding protein in the plasma that mediates the cellular uptake of cbl. although the synthesis of tcii by a variety of cultured mammalian cells and by some isolated perfused organs has been reported, no single tissue has been identified as the source of tcii in vivo. in this study, we demonstrate that cultured human umbilical vein endothelial cells secrete a protein that binds cn[57co]cbl, elutes from a sephacryl s-200 column in the same position as ... | 1989 | 2493194 |
| experimental transmission of crimean-congo hemorrhagic fever virus by hyalomma truncatum koch. | larval hyalomma truncatum ticks were infected with crimean-congo hemorrhagic fever (cchf) virus by allowing them to engorge on viremic newborn mice. the overall tick infection rate was 4.4% (24/542). virus was detected in specimens for greater than or equal to 160 days postinfection. transstadial transmission to the adult tick stage was observed and horizontal transmission to a mammalian host was demonstrated. horizontal transmission of cchf virus to uninfected adult ticks occurred while feeding ... | 1989 | 2493203 |
| monitoring of intestinal transplant rejection. | our results show that maltase, sucrase, and lactase activity are present at a normal level in nonrejecting small bowel transplants after an initial postoperative decline. this confirms that the disaccharide absorbing capacity of these grafts is intact. in allogeneic bowel, however, the levels of maltase and sucrase decline as histologic rejection occurs. these results suggest that serial maltase, sucrase, and possibly lactase levels in allogeneic intestinal transplants may serve as a useful adju ... | 1989 | 2493205 |
| in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine. | in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ... | 1989 | 2493210 |
| nuclear membrane lipid peroxidation products bind to nuclear macromolecules. | ascorbate-fe2+-driven lipid peroxidation processes in isolated rat liver nuclei give rise to products that bind to dna and total nuclear proteins. this has been demonstrated by integrating [3h]arachidonic acid into the nuclear membranes. lipid peroxidation was estimated from the formation of 2-thiobarbituric acid chromophore, and from the relative distribution of 3h-peroxidation products between the lipidic fraction and the nonlipidic fraction of the nuclear suspensions during incubation. the am ... | 1989 | 2493218 |
| alexandre yersin: pursuer of plague. | | 1989 | 2494200 |
| chloride and osmotic water permeabilities of isolated rabbit renal papillary surface epithelium. | the papillary surface epithelium (pse) covers the mammalian renal papilla. it has been proposed that water or solute fluxes across the pse might result in changes in urine or medullary interstitial fluid composition. to study osmotic water and chloride permeabilities, the pse was dissected from underlying medullary tissue and mounted in a small ussing chamber. osmotic water permeability was low (14.2 +/- 3.0 microns/s) and was unaffected by 100 nm vasopressin added to the basolateral surface. in ... | 1989 | 2504054 |
| detection of neisseria meningitidis and yersinia pestis with a novel silicon-based sensor. | a light-addressable potentiometric (silicon) sensor was used in an immunofiltration procedure for the detection of pathogenic bacteria. yersinia pestis was detected by filtering the cells onto nitrocellulose membranes and then filtering anti-y. pestis mouse monoclonal antibody and anti-mouse immunoglobulin g-horseradish peroxidase conjugate. for neisseria meningitidis detection, mouse monoclonal antibody to the major outer membrane protein of this bacterium was coupled directly to horseradish pe ... | 1989 | 2504767 |
| [possible control of capsule formation and intracellular synthesis of envelope antigen by each different plasmid]. | variants which lacked capsular envelopes on their cell surface were isolated from the culture of a highly virulent yreka strain of yersinia pestis grown in the presence of acridine orange, ethidium bromide or sodium dodecyl sulfate at incompletely growth-inhibitory concentrations. the variant could be divided into two types on the basis of the presence and the absence of intracellular envelope antigen. both types of the variants lacked the 13 megadaltone (md) plasmid. thus, it may well be said t ... | 1989 | 2504836 |
| [isolation of monoclonal antibodies for the identification of strains of the causative agent of plague that do not contain a capsular antigen]. | in most cases the immunological identification of y. pestis strains is based on the use of capsular antigen as an immunological marker. however, there are y. pestis strains without capsular antigen. for the immunological identification of such strains, homogeneous antigen with a molecular weight of 43 kd has been isolated and monoclonal antibodies to it have been obtained. the enzyme-linked immunosorbent assay, carried out with the use of these monoclonal antibodies and intended for the detectio ... | 1989 | 2511708 |
| direct activation of human polymorphonuclear leukocytes by pseudomonas aeruginosa. | brief exposure of polymorphonuclear leukocytes (pmns) to unopsonized pseudomonas aeruginosa resulted in significant enhancement of pmn adherence and 3h-deoxyglucose uptake, two important indicators of pmn activation. dose-response and time-course experiments show that maximal stimulation of pmns occurred at a pmn: bacteria ratio of 10:1 and exposure time of 10 min. the pmn-stimulatory capacity of p. aeruginosa was completely or partially abolished by treatment with formalin, heat, ultrasonicatio ... | 1989 | 2515158 |
| [studies of the antigenic structure of trichophyton equinum]. | in the studies on antigenic structure of t. equinum double diffusion precipitation test in agar gel and immunoelectrophoresis were used. while preparing precipitation sera in rabbits it was manifested that the best results are achieved by immunization with live antigen. in the group of metabolic antigens and water extracts of the strains of t. equinum which were tested, 10-11 serologically+ active fractions were found whereas in protein component only l1 fraction. in comparative studies based on ... | 1989 | 2518619 |
| [iron assimilation by yersinia pestis on iron-deficient media]. | the growth of plague bacteria may be limited by the level of iron concentration in the nutrient medium. the virulent strains of the plague microbe possess the more pronounced mechanism of iron assimilation as compared to the vaccine strain. the iron ions are extracted by the virulent and vaccine strains only under the cell surface contact with the iron-saturated transferrin. the iron-sorbing function is peculiar to the plague microbe cell walls which is pronounced more strongly in the virulent s ... | 1989 | 2524638 |
| the effect of cyclosporin a in murine visceral leishmaniasis. | leishmania donovani is an obligate intracellular protozoan parasite of macrophages whose control is effected by cellular mechanism. in order to determine the role of t-helper cells and their soluble mediators in the control of the infection, the effects of cyclosporin a (csa) were studied in strains of mice (c57l/j and c57bl/6j) known to be resistant and susceptible. mice treated with csa (200 mg/kg) exhibited as early as day 15 a significant increase in the level of their infection. the number ... | 1989 | 2525802 |
| a yersinia pestis-specific dna fragment encodes temperature-dependent coagulase and fibrinolysin-associated phenotypes. | the effect of temperature on coagulase and fibrinolysin expression (pla) by yersinia pestis has been implicated in the transmission of plague by fleas. in an attempt to improve our understanding of this process, we have cloned, sequenced and characterized the gene encoding the pla phenotypes in y. pestis, and examined its temperature-dependent regulation. the coding region for this gene overlaps a 900bp y. pestis-specific dna fragment that we have previously shown to be capable of detecting plag ... | 1989 | 2526282 |
| the role of thromboxane a2 in experimental and human models of chronic glomerular damage. | | 1989 | 2526467 |
| immunogenicity of a yeast-derived hepatitis b vaccine in hemodialysis patients. | in a multicenter study of hemodialysis patients in spain, the immunogenicity of a yeast-derived recombinant deoxyribonucleic acid hepatitis b vaccine was evaluated. two different vaccination schedules were examined: zero, one, two, six months and zero, one, two, 12 months. two different dose levels (20 micrograms and 40 micrograms) were also compared. no serious adverse effects were reported by any of the vaccinees; the most frequently reported reaction was soreness at the injection site. this s ... | 1989 | 2528295 |
| characterization of three functional sites in alpha beta 1 dr of drw13. all three sites are potentially involved in major histocompatibility complex-peptide interaction. | an hla-dr product encoded by the hla-drw13/dw19 haplotype has been identified as the hla class ii molecule involved in antigen presentation to several influenza-specific helper t cell clones. three different functional sites were identified on this molecule by comparing the structure of hla-dr products of known sequences and their ability to efficiently present foreign antigen to the t cell clones. these functional sites were mapped on the recently proposed three-dimensional structure of hla cla ... | 1989 | 2529124 |
| long-term analysis of data on the isolation of yersinia pestis cultures from rodents during epizootological examinations of natural foci in muyun-kum and eastern kizil-kum. | results are presented of a long-term examination and evaluation of data on the microbiological procedures of isolating yersinia pestis cultures from wild mammals and their association animal plague pathogenesis as suggested by investigations in the muyun-kum autonomous plague focus and the eastern kizil-kum mesofocus. 1772 yersinia pestits cultures were isolated largely from rhombomys opimus as the principle host over 23 years. the authors determined the frequency of pathological alterations in ... | 1989 | 2533934 |
| outer membrane peptides of yersinia pestis mediating siderophore-independent assimilation of iron. | it is established that wild-type cells of yersinia pestis absorb exogenous hemin or congo red and thus grow as pigmented colonies at 26 degrees c on media containing these chromatophores (pgm+). pgm+ isolated are known to possess a siderophore-independent mechanism of iron-transport (required for growth in iron-deficient medium) which is absent in avirulent pgm- mutants. production of the bacteriocin pesticin and linked invasins (pst+) is an additional defined virulence factor of yersiniae; muta ... | 1989 | 2535180 |
| correlation between selective inhibition of the cyclic nucleotide phosphodiesterases and the contractile activity in human pregnant myometrium near term. | the present study was carried out to determine the ability of various pharmacological agents to selectively inhibit each cytosolic form of phosphodiesterase isolated from the longitudinal layer of human myometria near term. among the drugs tested, zaprinast specifically inhibits the first form of pde which hydrolyses both substrates (camp and cgmp) and is stimulated by the ca2+-calmodulin complex. a second form of pde specific for camp hydrolysis and ca2+-calmodulin insensitive is only present d ... | 1989 | 2535936 |
| adeno-associated virus rep protein synthesis during productive infection. | adeno-associated virus (aav) rep proteins mediate viral dna replication and can regulate expression from aav genes. we studied the kinetics of synthesis of the four rep proteins, rep78, rep68, rep52, and rep40, during infection of human 293 or kb cells with aav and helper adenovirus by in vivo labeling with [35s]methionine, immunoprecipitation, and immunoblotting analyses. rep78 and rep52 were readily detected concomitantly with detection of viral monomer duplex dna replicating about 10 to 12 h ... | 1989 | 2536109 |
| bile acid profile in a dog with cholangiocarcinoma. | | 1989 | 2536512 |
| site specificity of the chorionic gonadotropin n-linked oligosaccharides in signal transduction. | the role of the human chorionic gonadotropin (hcg) n-linked oligosaccharides in receptor binding and signal transduction was analyzed using site-directed mutagenesis and transfection studies. hcg derivatives with alterations at individual glycosylation sites were expressed in chinese hamster ovary cells. receptor binding studies showed that absence of any or all of the hcg n-linked oligosaccharides had only a minor effect on the receptor affinity of the derivatives. similarly, absence of the n-l ... | 1989 | 2536708 |
| functional vip receptors in the human mucus-secreting colonic epithelial cell line cl.16e. | a polarized human clonal intestinal cell line exhibiting mucus secretion (cl.16e) was used to study the expression and function of vasoactive intestinal peptide (vip) receptors in mucus-secreting cells. cl.16e cells expressed one class of receptors with a kd of 0.13 nm and a capacity of 67 fmol/mg protein. covalent labeling of receptors followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a receptor protein with a mr of 63,000 in cl.16e cells. vip stimulated adenylate cyc ... | 1989 | 2538074 |
| mechanisms of inhibitory noradrenergic transmission in the rabbit facial vein. | in isolated buccal segment of the rabbit facial vein, electrical responses produced by perivascular nerve stimulation and exogenously applied noradrenaline (na) were recorded from the smooth muscle cells using microelectrode. perivascular nerve stimulation hyperpolarized the smooth muscle cell membrane. the hyperpolarization was converted to depolarization after application of the beta-adrenoceptor antagonist, propranolol, and the depolarization was blocked by alpha 2-adrenoceptor antagonists, y ... | 1989 | 2538798 |
| alloxan diabetogenicity: determinants of potentiation, protection and b-cell selectivity. | compounds with a reported in vivo and in vitro effect on the diabetogenicity of alloxan were studied with regard to the uptake of calcium in mouse islet mitochondria, with the aim of obtaining information on the susceptibility and selectivity of alloxan toxicity. a strong correlation was found between the uptake of calcium in mouse islet mitochondria, which is believed to be associated with the activation of oxidative enzymes involved in energy production and secretion of insulin, and the protec ... | 1989 | 2542107 |
| molecular cloning and heterologous expression of a klebsiella pneumoniae gene encoding alginate lyase. | the alginate lyase (aly; guluronate specific)-coding gene of klebsiella pneumoniae was cloned using the cosmid vector pmmb33, transduced into escherichia coli and expressed in this host. four aly-positive clones with unstable phenotypes were identified out of 700 kanamycin-resistant transductants. a stable derivative of one of the clones was studied further and contained 12.1-kb of insert dna. the aly-coding gene (aly), still partially under the control of its native promoter, was localised with ... | 1989 | 2542130 |
| structure of the human genomic glutathione s-transferase-pi gene. | the complete human genomic glutathione-s-transferase-pi gene (gst-pi) was isolated from a lambda charon 4a bacteriophage library which was screened by hybridization to a human gst-pi cdna. we have sequenced 4261 bp which include the entire gst-pi gene as well as over 1200 bp of the 5' and 200 bp of the 3' flanking regions. the gst-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. primer extension experiments identified four possible transcription start points closel ... | 1989 | 2542132 |
| relationship between calcium loading and impaired energy metabolism during na+, k+ pump inhibition and metabolic inhibition in cultured neonatal rat cardiac myocytes. | this study tested the hypothesis that the initiating mechanism is a major determinant of the response to calcium (ca) accumulation in myocardium. cultured neonatal rat ventriculocytes were exposed to na+, k+ pump inhibition with 1 mm ouabain and metabolic inhibition with 20 mm 2-deoxy-d-glucose and 1 mm cyanide (dog-cn) for up to 2 h. microspectrofluorometry of myocytes loaded with fura-2 showed that ouabain resulted in a relatively rapid increase in [ca2+]i up to 2-3 microm (two to threefold ab ... | 1989 | 2542375 |
| multiple cooperative interactions constrain bpv-1 e2 dependent activation of transcription. | transcription directed by the bpv-1 long control region (lcr) is conditional upon activation by the virally encoded e2 protein. within the 1.0 kb lcr there are five separate regions, a to e, that contain e2 responsive enhancers. the smallest functional region, a, is only 38 bp and contains two copies of the consensus sequence acc(n)6ggt that is known to function as an e2 binding site in vitro. we show that a pair of these constitutes a minimal functional e2 responsive enhancer element but that t ... | 1989 | 2542891 |
| sequences that promote formation of catenated intertwines during termination of dna replication. | the normal sequence at which sv40 dna replication terminates (ter) is unusual in that it promotes formation of catenated intertwines when two converging replication forks enter to complete replication (weaver et al., 1985). here we show that yeast centromeric sequences also exhibit this phenomenon. cen3 caused accumulation of late replicating intermediates and catenated dimers in plasmids replicating in mammalian cells, but only when it was located in the termination region (180 degrees from ori ... | 1989 | 2542894 |
| beta-adrenergic reactivity in conscious doca-salt hypertensive rats. | the endocrine (plasma renin activity, insulin and adh) and hemodynamic responses (heart rate and mean arterial pressure) to isoprenaline infusion were examined in conscious deoxycorticosterone-salt hypertensive rats (ds) and compared with uninephrectomized-salt control rats (us). a dose-related rise in plasma renin activity and plasma insulin values was found in us rats, while no change in either parameter was observed in ds rats after 30 min of isoprenaline infusion. adh was not increased in us ... | 1989 | 2543000 |
| mammalian topoisomerase ii activity is modulated by the dna minor groove binder distamycin in simian virus 40 dna. | dna topoisomerases ii are nuclear enzymes that have been identified recently as targets for some of the most active anticancer drugs. antitumor topoisomerase ii inhibitors such as teniposide (vm-26) produce enzyme-induced dna cleavage and inhibition of enzyme activity. by adding to such reactions distamycin, a compound whose effects on dna have been extensively characterized, we investigated the effects of drug binding upon topoisomerase ii-mediated dna cleavage induced by vm-26. we have found a ... | 1989 | 2544590 |
| electrophysiological properties of isolated rat liver cells. | the electrophysiological properties of isolated rat liver cells were studied using the patch clamp method in whole-cell configuration. the membrane potential in isolated hepatocytes was -42 +/- 7 mv (n = 20). the input resistance (rin) and the time constant (tau m) were 51 +/- 17 m (the range of 34 to 180 m omega) (n = 20) and 4.2 +/- 1.0 msec (the range of 3 to 16.5 ms) (n = 20). assuming that the specific membrane capacitance is 1 microf/cm2, the membrane resistance and membrane capacitance we ... | 1989 | 2544611 |
| identification of additional virulence determinants on the pyv plasmid of yersinia enterocolitica w227. | this paper describes the mutagenesis of the pyv plasmid from yersinia enterocolitica w22703 (serotype o:9) with tn2507, a new element generating operon fusions. analysis of the mutants allowed the identification of an additional yop protein called yop20 and the mapping of yop20, yop44, yop48, and lcrv, the gene encoding the v antigen. the last gene appeared to be part of an operon that also may contain yop37 and yop44. at 37 degrees c, mutants affected in this operon grew poorly, irrespective of ... | 1989 | 2545628 |
| differential effects of the destruction of leydig cells by administration of ethane dimethane sulphonate to postnatal rats. | ethane dimethane sulphonate (eds) is a cytotoxic drug that selectively destroys leydig cells in adult testes. this study has examined the effect of a single injection of eds on the leydig cell populations present in the testes of rats aged 5, 10, or 20 days. microscopic examination of the tissue demonstrated that the fetal leydig cell population was destroyed at all ages, but that subsequent development of the adult population of leydig cells was not affected. whilst the destruction of the fetal ... | 1989 | 2546615 |
| a hyper-recombination mutation in s. cerevisiae identifies a novel eukaryotic topoisomerase. | a hyper-recombination mutation was isolated that causes an increase in recombination between short repeated delta sequences surrounding the sup4-omicron gene in s. cerevisiae. the wild-type copy of this gene was cloned by complementation of one of its pleiotropic phenotypes, slow growth. dna sequence of the clone revealed a 656 amino acid open reading frame capable of encoding a protein homologous to the bacterial type i topoisomerase. no homology was detected with previously identified eukaryot ... | 1989 | 2546682 |
| retinoic acid enhances vip receptor expression and responsiveness in human neuroblastoma cell, sh-sy5y. | retinoic acid (ra) induces partial differentiation of neuroblastoma (nb) cells in vitro. in the human nb line, sh-sy5y (a neuroblastic subclone of sk-n-sh), ra was previously shown to enhance the stimulatory (pge1) and inhibitory (opioid) regulation of adenylyl cyclase. since these cells are also sensitive to camp stimulation by vasoactive intestinal peptide (vip), we have tested the effects of ra on vip receptor expression and function. pretreatment of sh-sy5y cells with 10 microm ra over 6 day ... | 1989 | 2546814 |
| regulation of hepatic expression of igf i and fetal igf binding protein mrna in streptozotocin-diabetic rats. | hepatic mrna levels of insulin-like growth factor i (igf i) and of the fetal, nonglycosylated 32 kda igf-binding protein (bp) were analysed in diabetic, diabetic insulin- and igf i-treated rats as well as in age-matched, healthy control animals. igf imrna levels are reduced in diabetic rats and increased by insulin treatment. in contrast, the infusion of igf i does not significantly upregulate igf i mrna levels. fetal igf bp mrna expression is very low in healthy control animals, but high levels ... | 1989 | 2546824 |
| does dietary fibre stimulate intestinal epithelial cell proliferation in germ free rats? | the aim of the present experiment was to investigate the role of hind gut fermentation in the proliferative response of the intestinal epithelium to dietary fibre. we have previously shown that refeeding starved rats with an elemental diet supplemented with fermentable dietary fibre (but not inert bulk) is capable of stimulating intestinal epithelial cell proliferation throughout the gastrointestinal tract. three groups of 10 germ free (gf) rats and three groups of 10 conventional (cv) rats, wer ... | 1989 | 2546871 |
| pseudomonas elastase acts as a virulence factor in burned hosts by hageman factor-dependent activation of the host kinin cascade. | purified pseudomonas elastase injected subcutaneously into the skin of an evans blue dye-injected (intravenously) guinea pig caused dye leakage similar to that observed when histamine or bradykinin was injected in the same animal. the histamine-induced dye leakage was ablated in antihistamine-treated guinea pigs, but elastase- and bradykinin-induced dye leakages were not. local injections of specific inhibitors of the host hageman factor-dependent bradykinin-generating pathway given immediately ... | 1989 | 2553607 |
| hand-to-hand transmission of herpes simplex virus type 1. | droplets of tissue culture fluid containing herpes simplex virus type 1 were placed on the palm of the hand. each 0.01 ml droplet was taken from a stock virus suspension with a titre of 10(7.5) tcid50/0.1 ml. at 0, 15, 30, 60 and 120 min a droplet was firmly touched with the middle finger of the right hand, after which, attempts were made to recover virus from the finger. at 0 min, when the virus-containing droplet was in a liquid state, there was a 100% rate of virus recovery. by 15 min the dro ... | 1989 | 2554099 |
| effect of a noncompetitive antagonist (mk-801) of nmda receptors on convulsions and brain amino acid level in e1 mice. | anticonvulsant action of mk-801, a novel noncompetitive antagonist of n-methyl-d-aspartate (nmda) receptor, was examined in genetically epileptic e1 mice. systemic injection of mk-801 (0.1-1.0 mg/kg) potently suppressed generalized tonic-clonic convulsions of in a dose-dependent manner (ed50, 0.17 mg/kg). this anticonvulsant effect of mk-801 appeared at a dose which did not induce any obvious behavioral changes. following the administration of a fully anticonvulsant dose of mk-801 (1 mg/kg), ami ... | 1989 | 2554174 |
| human ht-29 colon carcinoma cells contain muscarinic m3 receptors coupled to phosphoinositide metabolism. | five different muscarinic receptor subtypes can be distinguished by the differences in their amino acid sequence, the coupled signal transduction system, pharmacological binding properties and activation of ionic fluxes. the present study served to characterize the binding profile of muscarinic receptors in human colon carcinoma cells (ht-29) using selective muscarinic antagonists. the affinities of the compounds were compared with their potency to inhibit cholinergically-activated phosphoinosit ... | 1989 | 2555210 |
| [assessment of diagnostic prospects for a method of immunoenzyme analysis during mass testing of feces of patients for the purpose of detecting rotavirus antigens]. | | 1989 | 2555970 |
| effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets. | the influence of glucocorticoid administration and limited nursing on piglet carbohydrase enzyme development and subsequent growth was examined in three experiments using 371 piglets. treatments in the first two experiments were formed by the factorial arrangement of hydrocortisone (-hyd or +hyd) and limited nursing (-ln or +ln) imposed form d 14 to weaning (d 28). hydrocortisone was replaced by adrenocorticotropic hormone (acth) in the third experiment. growth rates were severely depressed by h ... | 1989 | 2556355 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| cryotherapy and co2-laser vaporization in the treatment of cervical and vaginal human papillomavirus (hpv) infections. | a series of 119 women with human papillomavirus (hpv) infections of the uterine cervix and/or vagina were included in the present study, where the efficacy of cryotherapy and co2-laser vaporization was assessed after a mean follow-up of 14 months (sd 6 months) after treatment, as related to the natural history of the disease. routine papanicolaou (pap) smears with hpv-induced changes were the basis for patient recruitment. patients with cervical hpv lesions (hpv-ncin, hpv-cin i or ii) were rando ... | 1989 | 2561042 |
| effect of endogenous opioid peptides on trh release from rat stomach in vitro. | the effect of endogenous opioid peptides (beta-endorphin, leucine-enkephalin, dynorphin 1-13 on the release of thyrotropin releasing hormone (trh) from the rat stomach in vitro was studied. the rat stomach was incubated in the medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) (medium) and the amount of trh released into the medium was measured by radioimmunoassay. the release of trh from the rat stomach was inhibited significantly in a dose-related manner with the addition of endogenous opioid pe ... | 1989 | 2565806 |
| start and end of the effects of terfenadine and astemizole on histamine-induced wheals in human skin. | a study was made of effects of two antihistamines, terfenadine (60 mg twice daily) and astemizole (10 mg once daily) on wheals induced by histamine dihydrochloride (10 mg/ml) in the prick test on the upper back of 15 healthy students. the suppressive effects of terfenadine on the histamine wheal appeared earlier (2 h), and disappeared earlier (within 1 day) than those of astemizole (3 days and 28 days, respectively). no difference between the maximal effects of the two drugs was seen. | 1989 | 2566243 |
| the cd2 antigen associates with the t-cell antigen receptor cd3 antigen complex on the surface of human t lymphocytes. | t lymphocytes can be activated by various stimuli directed either against the t-cell antigen receptor-cd3 antigen complex (ti-cd3) or the cd2 molecule; see ref. 1 for a review. activation signals generated by antigen binding to the antigen-specific alpha/beta heterodimer (ti) are thought to be transduced via the invariant cd3 gamma, epsilon and delta chains, and the associated zeta and eta subunits. the physiological role of the interaction of cd2 with its homologous cell-surface associated liga ... | 1989 | 2567497 |
| cloning and structural characterization of the genes coding for adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii. | the structural genes coding for both subunits of adenosylcobalamin-dependent methylmalonyl-coa mutase from the gram-positive bacterium propionibacterium shermanii have been cloned, with the use of synthetic oligonucleotides as primary hybridization probes. the genes are closely linked and are transcribed in the same direction. nucleotide sequence analysis of 4.5 kb of dna encompassing both genes allowed us to infer the complete amino acid sequence of the two subunits: the beta-subunit is the pro ... | 1989 | 2569861 |
| gut perforation after infection with herpes simplex virus and epstein-barr virus. | | 1989 | 2570271 |
| caffeine toxicity is inversely related to dna repair in simian virus 40-transformed xeroderma pigmentosum cells irradiated with ultraviolet light. | human cells transformed by simian virus 40 (sv40) are more sensitive to killing by ultraviolet light when grown in caffeine after irradiation. the degree of sensitization at 2 mm caffeine (expressed as the ratio of the 37% survival dose for control cells divided by the 37% survival dose for cells grown in caffeine, i.e., the dose modification factor) was approximately 1.9 in transformed normal cells and 3.8-5.8 in excision-defective xeroderma pigmentosum (xp) groups a, c, and d cells. a large do ... | 1989 | 2570469 |
| vigabatrin therapy in patient with succinic semialdehyde dehydrogenase deficiency. | | 1989 | 2572834 |
| modulation of transmitter and hormone release by multiple neuronal opioid receptors. | | 1989 | 2573137 |
| leukotriene metabolism and hypersensitivity in man and mouse. | the relative rates of cysteinyl-leukotriene metabolism were analyzed in fresh human and mouse serum. human serum contained higher gamma-glutamyl-transpeptidase activity than mouse serum, and a higher percentage of the metabolized leukotriene c4 was recovered as leukotriene d4 in the human serum than in the mouse serum. the results suggest that the patterns of metabolism of the cysteinyl-leukotrienes could be an important factor in determining the relative sensitivity of an animal to the developm ... | 1989 | 2573348 |
| structure-activity relationships as a response to the pharmacological differences in beta-receptor ligands. | with a few notable exceptions, beta-receptor ligands (agonists and antagonists) belong to the aryl- or heteroaryl-ethanolamine series and to the aryl- or heteroaryl-oxypropanolamine series. structure-activity relationships for beta-adrenergic agonists show that a secondary amine in the phenylethanolamine side chain ending is essential for receptor stimulation. the 3,4-dihydroxyphenyl groups may be replaced by "phenol equivalents" (-ch2oh, -nhconh2, -choh, -nhso2ch3). in contrast, substitution at ... | 1989 | 2573372 |
| long-range mapping in the research and diagnosis of genetic disease. | this paper reviews current genetic and molecular biological methods that may be used in the so-called "reverse genetics" approach. these methods are the mapping, isolation, and study of the chromosomal dna containing a previously unidentified gene responsible for a genetic disease, beginning with its chromosomal localization. in principle, the reverse genetics methodology follows the same path for different diseases studied. an overall outline of the steps to be undertaken is given and discussed ... | 1989 | 2576651 |
| characterization of rat cerebral cortical beta adrenoceptor subtypes using (-)-[125i]-iodocyanopindolol. | (-)-[125i]-iodocyanopindolol [-)icyp), used to characterize beta adrenoceptors on membrane preparations from rat cerebral cortex, was shown to have affinity for both beta adrenoceptors and serotonin receptors. therefore, 10 microm serotonin was added to the assays to prevent (-)icyp binding to serotonin receptors. under these conditions, (-)icyp binding to the cortical membrane preparation was reversible and saturable, and the association reaction was very slow. the dissociation reaction was als ... | 1989 | 2577008 |
| functional differentiation of cell types of cortical collecting duct. | interference-contrast and fluorescent microscopy were used to differentiate between the two cell types--principal cells (pc) and intercalated cells (ic)--of the isolated perfused cortical collecting duct of the rabbit. using hoffman modulation contrast optics, two types of cell outlines could be identified: "hexagonal" and "circular" profiles. to characterize the cell types further, the binding of fluorescein-labeled peanut lectin, which has been shown to be specific for the luminal cell membran ... | 1985 | 2579583 |
| all hepatocytes are involved in the expression of the albumin gene in the normal adult rat: a demonstration by in situ hybridization and immunoperoxidase techniques. | immunoperoxidase techniques have yielded conflicting results concerning the percentage of hepatocytes engaged in albumin production in normal adult rats. in addition, the question of whether functional differences in the synthesis of plasma proteins exist within the hepatic lobule remains to be determined. to clarify these questions, we have searched for gene albumin transcripts by in situ hybridization, and for the corresponding protein by immunoperoxidase, on adjacent liver sections. we observ ... | 1985 | 2579741 |
| ion selectivity of gram-negative bacterial porins. | twelve different porins from the gram-negative bacteria escherichia coli, salmonella typhimurium, pseudomonas aeruginosa, and yersinia pestis were reconstituted into lipid bilayer membranes. most of the porins, except outer membrane protein p, formed large, water-filled, ion-permeable channels with a single-channel conductance between 1.5 and 6 ns in 1 m kcl. the ions used for probing the pore structure had the same relative mobilities while moving through the porin pore as they did while moving ... | 1985 | 2580824 |
| [basic somatic antigen or antigen obtained from yersinia pestis by the boivin-mesrobeanu method]. | | 1985 | 2581401 |
| expression of an hla-bw6-related specificity by the hla-cw3 molecule. | radioimmunoassay of hla-transformed mouse l cells expressing a3, a24, b7, or cw3 hla class i molecules with a set of monomorphic monoclonal antibodies distinguishes between a3-a24 and b7-cw3 patterns of reactivity. analyses with bw6-specific monoclonal antibodies and a human alloantiserum demonstrate the expression by the hla-cw3 molecules of a bw6 public specificity related to but not identical with that expressed by the hla-b7 molecules. exon-shuffling experiments and inhibition studies of mon ... | 1985 | 2581891 |
| an evaluation of the combined use of nuclear magnetic resonance and distance geometry for the determination of protein conformations in solution. | an evaluation of the potential of nuclear magnetic resonance (n.m.r.) as a means of determining polypeptide conformation in solution is performed with the aid of a new distance geometry program which is capable of computing complete spatial structures for small proteins from n.m.r. data. ten sets of geometric constraints which simulate the results available from n.m.r. experiments of varying precision and completeness were extracted from the crystal structure of the basic pancreatic trypsin inhi ... | 1985 | 2582141 |
| [use of hemin as a stimulator of the growth of the plague microbe]. | | 1985 | 2582200 |
| [the significance of detecting corynebacterium equi in the conjunctiva of children in cairo]. | the probable correlations between certain corynebacterium are discussed. carpenter et al. (1976) describes a corynebacterium equi-pneumonia in a patient with hodgkin's disease; gardner et. al. (1976) also reports on a case of pneumonia. golub et. al. (1967) demonstrates a lung abscess resulting from corynebacterium equi. williams et. al. (1971) demonstrates a thoracic infection in an immunosuppressed patient. although, our findings have been demonstrated only in conjunctivitis of children, hithe ... | 1989 | 2583507 |
| staphylococcal arthritis--effects of superoxide dismutase on infected knee joints of rabbits. | the effect of sod on staphylococcal arthritis has not been successfully evaluated to date. we developed an animal model to investigate the correlation. using 16 rabbits divided into four groups, we injected two groups with staphylococcus aureus and the other two with nacl. one group in each was also injected with sod. the presence of sod activity in untreated and infected knee joints of rabbits over a period of 72 hours showed no significant difference. tba-reactive substances (tbars) measured i ... | 1989 | 2583550 |
| [production of human monoclonal auto-antibodies which recognize skin components]. | using human myeloma cell line, licr-lon-hmy2, and human lymphocytes, we have produced human monoclonal auto-antibodies against skin-components by human-human hybridoma technique. resulting monoclonal antibodies are classified as follows; 1. antibodies which recognize cytoplasmic filamentous antigen(s) in epithelial and non-epithelial cells, which are presumably keratin cross-reacting with other classes of intermediate filament(s), 2. antibody to keratinocyte cellular membrane which is specific t ... | 1989 | 2585779 |
| [nuclear dna cytofluorometry of normal human laryngeal epithelia and squamous cell carcinoma]. | normal human laryngeal epithelia and laryngeal squamous cell carcinoma were assayed by feulgen dna cytofluorometry using free cell nuclei isolated from carnoy-fixed, paraffin-embedded specimens. in all of the 12 normal specimens, the epithelium showed typical diploid cell clones with low proliferative activity. polyploid cells were seen in only two specimens from subjects aged 61 and 69 years respectively, and the number of polyploid cells seen in these two specimens was only two. fifteen cancer ... | 1989 | 2593035 |
| [dna analysis for the kininogen gene of patients with kininogen deficiency in japan]. | the kininogen (kgn) gene status was examined in 4 families with both high molecular weight (hmw) and low molecular weight (lmw) kgm deficiency and one family with only hmw-kgn deficiency reported in japan. no abnormal hmw-kgn or lmw-kgn was detected in those with these deficiencies by immunoblot analysis using monoclonal antibodies (hkg-h12, hkg-l7, hkg-l17) for human hmw-kgn. hmw-dna prepared from peripheral blood leucocytes was digested with endonuclease, ecori, bam hi, hind iii, sca i, bg1ii, ... | 1989 | 2593235 |
| an original inflow cannula for paracorporeal assist devices. | cannulae for paracorporeal ventricular assist devices have been clinically shown to be limiting factors in optimal circulatory support. a new inflow cannula has been manufactured according to an original silicone rubber molding process, permitting a hemocompatible inner surface. the cannula is 200 mm in length, with a 10 mm internal diameter. the wall is 3 mm thick and reinforced by a stainless steel spiral spring embedded in the silicone. an external ring permits adequate interface between the ... | 1989 | 2597500 |
| testicular feminization in a cat. | | 1989 | 2599922 |
| differential blocking effects of atropine and gallamine on negative chronotropic and dromotropic responses to vagus stimulation in anesthetized dogs. | the negative dromotropic response to cervical vagus stimulation was inhibited less than the negative chronotropic response to cervical vagus stimulation by the same dose of atropine in neurally decentralized, anesthetized dogs. atropine and gallamine bind different muscarinic binding sites. we thus investigated the blocking effects of atropine and gallamine on the negative chronotropic and dromotropic responses to stimulation of the vagus nerves. we stimulated the intracardiac vagus nerves to th ... | 1989 | 2600814 |
| electrophoretic characterisation of the outer membrane proteins of yersinia pestis isolated in north-east brazil. | the outer membrane proteins of 38 yersinia pestis isolates from all known plague foci of north-east brazil were analysed by sds-page. approximately 20 bands were consistently found in all strains analysed and 11 were selected for comparative studies. although qualitative differences among the electrophoretic profiles of outer membrane proteins of wild y. pestis isolates were not observed, quantitative alterations were clearly noted for most of these proteins. no particular quantitative alteratio ... | 1989 | 2606164 |
| rapid methods for purification of human recombinant interleukin-5 (il-5) using the anti-murine il-5 antibody-coupled immunoaffinity column. | a large scale production of human recombinant il-5 (hil-5) was performed by way of recombinant dna technology. in this study, we transfected chinese hamster ovary cells with pdkcr-hil-5gene-dhfr plasmid and selected a cell line, with the use of methotrexate, producing large amounts of hil-5. the recombinant hil-5 thus obtained induced igm production of murine b cell leukemia bcl1, and its activity was inhibited by tb13 anti-mouse il-5 monoclonal antibody. hil-5 could be purified from the cell-fr ... | 1989 | 2607155 |
| reciprocal inhibition following lesions of the spinal cord in man. | 1. reciprocal inhibition was studied in normal subjects and patients with spinal cord lesions by stimulating the posterior tibial nerve below the threshold of the soleus alpha-motoneurone axons and recording the changes in firing probability of single tibialis anterior motor units activated by voluntary contraction. a short-latency (about 35 ms) period of decreased firing probability was assumed to represent reciprocal inhibition. 2. for a given stimulus intensity this inhibition was greater in ... | 1989 | 2607428 |
| [the participation of the adrenergic receptors of the peritoneal leukocytes from white mice in the mechanism of the cellular control of the action of yersinia pestis adenylate cyclase]. | y. pestis extracellular adenylate cyclase suppresses the oxidation metabolism of peritoneal leukocytes in white mice. the character of the modulating action of the enzyme in its interaction with the target cell infers the participation of adrenergic receptors. | 1989 | 2609813 |
| three dimensional observations of the palisade-shaped nerve endings of normal hair of rat's snout. | the nerve endings of normal hair of the rat's snout, partially digested with trypsin and hydrochloric acid, were studied by scanning electron microscopy. each lanceolate structure measured ca. 10 microns in length and was arranged around the hair follicle. these palisade-shaped nerve endings were situated almost beneath the sebaceous glands, ran upward, parallel to the axis of the hair follicle, and terminated in pointed shape. 2 kinds of cells, teloglia cell type i showing flat profile, and tel ... | 1989 | 2610376 |
| regional difference in airway epithelial response to neutrophil elastase: tracheal secretory cells discharge and recover in hamsters that develop bronchial secretory-cell metaplasia. | human neutrophil elastase (hne) causes secretory granule discharge and conversion of many clara cells to mucous cells in hamster bronchi. we investigated whether the trachea responds to hne in a similar manner because of its abundance of clara cells. by light microscopy, the tracheal epithelium of animals exposed to a single intratracheal injection of hne was normal at 21 days, although bronchial secretory-cell metaplasia (scm) was present. an ultrastructural differential cell count showed no in ... | 1989 | 2612449 |