| [systemic vasculitis combined with connective tissue involvement in a patient with yersiniosis]. | a clinico-morphological description is presented of a severe systemic disease in 36-year-old male, the trigger factor of which was yersinia enterocolitica. the patient developed a peculiar nosological variant of systemic necrotic vasculitis of infectious-allergic genesis with signs of nodular periarteritis in the intestine and kidneys in association with diffuse involvement of the connective tissue which resulted 6 months later in a dominating abdominal syndrome with profuse diarrhea, progressiv ... | 1991 | 1683069 |
| immunoglobulins of patients recovering from yersinia enterocolitica infections exhibit graves' disease-like activity in human thyroid membranes. | substantial evidence suggests a link between infections with yersinia enterocolitica (ye) and graves' disease. we have now examined the sera of 72 patients recovering from ye infection for immunoglobulins that interacted with the tsh receptor in human thyroid membranes. compared with controls, in concentrations between 1 and 4 mg/ml, patient igg produced a significant, concentration-dependent inhibition of tsh binding (p less than 0.001) and stimulation of adenylate cyclase activity (p less than ... | 1991 | 1688156 |
| nucleotide sequence of the yersinia enterocolitica ail gene and characterization of the ail protein product. | the ability to enter (invade) eucaryotic cells is a property common to many pathogenic bacteria. yersinia enterocolitica is a facultative intracellular pathogen whose primary site of multiplication is the reticuloendothelial system. in an effort to understand how y. enterocolitica crosses the intestinal epithelial cell layer, we previously reported the cloning of two loci from y. enterocolitica that individually conferred an invasive phenotype to the normally noninvasive escherichia coli hb101. ... | 1990 | 1688838 |
| hc-iga antibodies of different specificities are normally present in serum: quantitation by elisa and relationship to the major ig classes. | enzyme-linked immunosorbent assays (elisa) were developed for direct measurement of protein hc-iga complexes (hc-iga) in serum with antibody specificity for rabbit igg (rheumatoid factor (rf) activity), lipopolysaccharide from yersinia enterocolitica serotype o:3 (y3) and tetanus toxoid (tt). about 80% of patients with rheumatoid arthritis had increased concentrations of hc-iga-rf. the values were correlated with the concentrations of iga-rf and igm-rf. hc-iga anti-y3 was measured in 45 sera wit ... | 1990 | 1690557 |
| nonpathogenic isolates of yersinia enterocolitica do not contain functional inv-homologous sequences. | previous studies have demonstrated a correlation between the ability of isolates of yersinia enterocolitica to cause disease and to invade tissue culture cells in vitro. two genes, inv and ail, isolated from a pathogenic strain of y. enterocolitica have each been shown to confer this invasive phenotype upon escherichia coli. eighty pathogenic, invasive isolates studied by miller et al. (infect. immun. 57:121-131, 1989) contained sequences homologous to both of these genes. thirty-five nonpathoge ... | 1990 | 1690684 |
| identification of the integrin binding domain of the yersinia pseudotuberculosis invasin protein. | the invasin protein of the pathogenic yersinia pseudotuberculosis mediates entry of the bacterium into cultured mammalian cells by binding several beta 1 chain integrins. in this study, we identified the region of invasin responsible for cell recognition. thirty-two monoclonal antibodies directed against invasin were isolated, and of those, six blocked cell attachment to invasin. these six antibodies recognized epitopes within the last 192 amino acids of invasin. deletion mutants of invasin and ... | 1990 | 1693333 |
| [the structure of a repetitive unit of the glycerolphosphate- containing o-specific polysaccharide chain from yersinia kristensenii strain 103 (0:12,26) lipopolysaccharide]. | mild acid hydrolysis of the lipopolysaccharide from yersinia kristensenii strain 103 (0:12.26) afforded teichoic acid-like polysaccharide. from the results of methylation, dephosphorylation, partial smyth degradation, and 13c and 31p nmr data the structure of the repeating unit of the polysaccharide was deduced as follows: [formula: see text] the structure was confirmed by complete interpretation of polysaccharide 13c nmr spectrum. | 1990 | 1694078 |
| structural and functional similarities between the replication region of the yersinia virulence plasmid and the repfiia replicons. | we sequenced the minimum replication region of the virulence plasmid pyve439-80 from a serogroup o:9 yersinia enterocolitica. this sequence is 68% homologous on a 1,873-nucleotide stretch to the sequence of the repfiia replicon of the resistance plasmid r100. the sequence contains two open reading frames, repa and repb, encoding proteins of 33,478 and 9,568 daltons, respectively. the amino acid sequences of the two proteins are 77 and 55% identical, respectively, to proteins repa1 and repa2 of t ... | 1990 | 1694522 |
| the yersinia enterocolitica inv gene product is an outer membrane protein that shares epitopes with yersinia pseudotuberculosis invasin. | an essential virulence attribute for yersinia enterocolitica and yersinia pseudotuberculosis is the ability to invade the intestinal epithelium of mammals. the chromosomal invasin gene (inv) has been cloned from both of these yersinia species, and the y. pseudotuberculosis invasin has been well characterized (r. r. isberg, d. l. voorhis, and s. falkow, cell 50:769-778, 1987). here we constructed tnphoa translational fusions to the y. enterocolitica inv gene to identify, characterize, and localiz ... | 1990 | 1694524 |
| o-serotyping of yersinia ruckeri with special emphasis on european isolates. | yersinia ruckeri is the aetiological agent of enteric redmouth disease (erm), an acute to chronic bacteraemic infection of salmonid fish. the o-serotypes of 127 isolates of y. ruckeri obtained from europe (96 isolates), north america (23 isolates), australia (six isolates) and south africa (two isolates), as well as four reference strains, were determined by slide agglutination test and microplate agglutination assay. a serotyping scheme is proposed based on heat-stable o-antigens; the serotypes ... | 1990 | 1694607 |
| in vitro immunostimulation of rainbow trout (oncorhynchus mykiss) spleen cells with levamisole. | an in vitro immunization and cultivation method for fish spleen organ sections was used to investigate the effects of levamisole on the immune response. after 10 days of culture with either 50 micrograms/ml, 25 micrograms/ml, 5 micrograms/ml or no levamisole in the media, the nonspecific defense reactions were measured by determining the metabolic activity of neutrophils by using the nitroblue tetrazolium test, and phagocytic and adherence indexes by incubating the fish cells with suspensions of ... | 1990 | 1695158 |
| lcrr, a low-ca2(+)-response locus with dual ca2(+)-dependent functions in yersinia pestis. | the low-ca2+ response (lcr) of yersinia includes a regulatory cascade and a set of virulence-related proteins, one of which is the v antigen. the regulatory genes modulate both bacterial growth and expression of the virulence-related proteins in response to temperature and the presence of ca2+ and nucleotides. in this study we defined a new lcr locus, lcrr, in yersinia pestis kim. an lcrr mutant, obtained by insertion mutagenesis, failed to grow at 37 degrees c whether ca2+ was present or not. h ... | 1990 | 1695896 |
| [cytopathogenic properties of yersinia pestis strains in an l-41 cell culture]. | l-41 cell monolayer grown in cultural microplates was infected with y. pestis strains of different virulence. the results evidenced a direct relationship between the strains' virulence for white mice and their ability to multiply in cellular system and to show cytopathogenic effect. | 1990 | 1695978 |
| [a liquid nutrient medium for isolating yersinia]. | | 1990 | 1695979 |
| production of a monoclonal antibody to vibrio cholerae non-o1 heat-stable enterotoxin (st) which is cross-reactive with yersinia enterocolitica st. | a monoclonal antibody (mab) against synthetic heat-stable enterotoxin of vibrio cholerae non-o1 (nag-st) was produced. the mab, namely, 2f, belonged to the immunoglobulin g1 class. ascitic fluid drawn from pristane-primed balb/c mice injected with a 2f-producing clone demonstrated anti-nag-st activity which could be detected in enzyme-linked immunosorbent assay even at a dilution of 1:128,000. fifty-fold-diluted ascitic fluid could completely neutralize the activity of nag-st (synthetic and nati ... | 1990 | 1696928 |
| [primary structure of the 5'-region of yersinia pestis 16s rrna]. | the sequence of 5'-region (16-296 n.) of 16s rrna of plague agent (yersinia pestis) was determined after sequencing of cloned cdna fragments complementary to this region. when compared to the same region of 16s rrna of escherichia coli and proteus vulgaris this region revealed 91.8% and 87.2% of homology, respectively. the sequences specific for y. pestis 16s rrna were localized and their secondary structure position was discussed. | 1990 | 1698255 |
| o-antigen biotin conjugates. preparation and use in direct competitive enzyme immunoassays. | bacterial o-antigens coupled to biotin were shown to function well as labelled antigens in direct enzyme immunoassay. o-polysaccharides released from lipopolysaccharides by mild acid hydrolysis were oxidized by sodium periodate at sites located within the lipopolysaccharide inner core region and the generated aldehyde groups were subjected to reductive amination with 1,3-diaminopropane to yield per-aminated o-polysaccharide derivatives. biotin was coupled to the introduced amino groups by way of ... | 1990 | 1698876 |
| a tetrapeptide shared by yersinia outer membrane protein yop1 and hla-b27. antibody response in patients with yersinia-triggered reactive arthritis. | | 1990 | 1701921 |
| nucleotide sequence of the yersinia pestis gene encoding f1 antigen and the primary structure of the protein. putative t and b cell epitopes. | the plasmid-located gene caf1 encoding the capsular antigen fraction 1 (f1) of yersinia pestis was cloned and sequenced. the gene codes for a 170 amino acid peptide with a deduced mr of 17.6 kda. the signal peptide sequence was highly homologous to the e. coli consensus signal sequence. the f1 was assumed to have beta-sheet structure for the most part. the region located between amino acids 100 and 150 was suggested to contain putative antigenic determinants and to stimulate t cells. | 1990 | 1702734 |
| [the pyrazinamidase activity of bacteria of the genus yersinia]. | a modified nutrient medium, made of reagents and standard media manufactured in this country, tried in practice, is suggested for yersinia pyrazinamidase test. tisamid (finland), a pyrazinamide antituberculosis agent was used as substrate. a positive test is characteristic of nonpathogenic and a negative one for pathogenic yersinia. the test and the medium may be effectively used in laboratory diagnostic studies for identification of yersinia. | 1990 | 1702871 |
| [a diagnostic serum with antibodies to virulent yersinia]. | a diagnostic agglutinating adsorbed rabbit serum with antibodies to yersinia strains containing virulence plasmid with molecular mass of 40-50 md was prepared. trials of this serum in agglutination test on the glass with 69 yersinia strains (y. enterocolitica, y. frederiksenii, y. intermedia, y. kristensenii, y. pseudotuberculosis) and with 42 other enterobacteriaceae strains have confirmed the specificity and sufficiently high activity of the agent in respect of virulent yersinia strains. exper ... | 1990 | 1704466 |
| [the antigenic relationship between brucella abortus, brucella melitensis and yersinia enterocolitica serotype 0:3 and 0:9]. | antigenic interrelationship between b. abortus, b. melitensis, y. enterocolitica serotype 0:3 and serotype 0:9 were investigated in hyperimmün sera obtained from rabbits, by using agglutination and agglutinin absorbtion tests. agglutination was determined at 1/2560 titer of b. abortus and b. melitensis hyperimmün sera when their own antigens were used. on the other hand, these sera agglutinated y. enterocolitica serotype 0:9 antigen at 1/1280 and 1/320 titer respectively. as it was seen that hig ... | 1990 | 1704479 |
| [quantitative determination of the carbohydrate determinants of o-antigens of yersinia pseudotuberculosis and pseudomonas fluorescens]. | interactions of o-specific polysaccharides, lps and lps-protein complex with specific antibodies isolated on a polysaccharide-substituted immunoadsorbent gel and monovalent fab-fragments of the antibodies were investigated. the association constants and quantity of fab-fragments bound to these antigens were established. the interaction of the lps and lps-protein complexes was shown to depend considerably on concentration of antigens, and only 10-15% of carbohydrate determinants are accessible fo ... | 1990 | 1704703 |
| tyrosine phosphate hydrolysis of host proteins by an essential yersinia virulence determinant. | the plasmid-encoded yoph protein is a protein-tyrosine phosphatase (ptpase; ec 3.1.3.48) that is essential for yersinia virulence. we have investigated the molecular basis for the role of ptpase activity in yersinia pathogenesis. allelic recombination was employed to introduce a defined mutation into the yoph plasmid gene. conversion of the essential cys-403 to ala in the catalytic domain of the protein abolished yoph ptpase activity and significantly reduced the virulence of yersinia pseudotube ... | 1991 | 1705028 |
| analysis of the v antigen lcrgvh-yopbd operon of yersinia pseudotuberculosis: evidence for a regulatory role of lcrh and lcrv. | virulent yersinia species possess a common plasmid that encodes essential virulence determinants (yops) which are regulated by the extracellular stimuli ca2+ and temperature. the v antigen operon was recently shown to be involved in the ca2(+)-regulated negative pathway (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). we show here that the v antigen-containing operon of yersinia pseudotuberculosis is a polycistronic operon having the gene order lcrgvh-yopbd. dna sequencing analys ... | 1991 | 1705541 |
| [studies on the serogroup of yersinia enterocolitica. ii. selection and establishment on serogroup reference strains of yersinia enterocolitica]. | since 1980, we have collected 1120 strains of yersinia enterocolitica, from the different parts of china. these strains have been obtained from various sources in man, animals and natural environment accompanied by their clinical or ecological information of yersinia enterocolitica. the results of our tests have shown that the 747 strains have exhibited the clinical morphological and biochemical characteristics of yersinia enterocolitica. through comparing under the same conditions, out of the 7 ... | 1991 | 1709767 |
| an o antigen can interfere with the function of the yersinia pseudotuberculosis invasin protein. | escherichia coli strains harbouring the yersinia pseudotuberculosis inv gene are able to enter cultured mammalial cells. we show here that this property is not shared by all enteric bacteria, since shigella flexneri 2a cured of its virulence-associated plasmid and harbouring the inv gene is unable to enter mammalian cells efficiently. mapping studies showed that the region of the chromosome responsible for this phenotype includes rfab, a locus involved in the production of o antigen. s. flexneri ... | 1991 | 1710312 |
| [the use of growth stimulants in the bacteriologic diagnosis of yersinia]. | the effects of k. s. karpuzidi's stimulants (0.5-1 percent) and of para-amino benzoic acid (0.1-05 mm) on the growth and multiplication of 3 yersinia pseudotuberculosis, 1 y. enterocolitica strains, and e. coli nonpathogenic strain were examined. karpuzidi's stimulant was found to selectively stimulate yersinia cell growth and inhibit escherichia development in conditions unfavorable for these bacteria. para-amino benzoic acid intensively stimulated the growth of both yersinia and escherichia. w ... | 1991 | 1710732 |
| [a differential diagnostic medium for the isolation of bacteria of the yersinia genus]. | a formula of a solid differentiating diagnostic media for the isolation of yersinia genus bacteria is suggested. the medium does not include components hard to get and is simple to prepare. no additional sterilization is necessary after the preparation, after the medium is poured into petri dishes, it is dried in the air. the medium inhibits the growth of cocci and gram-negative contaminant microflora. yersinia bacteria may be easily differentiated by the color and typical morphology of the colo ... | 1991 | 1710733 |
| intervening sequences (ivss) in the 23s ribosomal rna genes of pathogenic yersinia enterocolitica strains. the ivss in y. enterocolitica and salmonella typhimurium have a common origin. | the 23s ribosomal rna (rrna) was shown to be in two fragments in pathogenic yersinia enterocolitica. the cleavage site in the structural gene of the 23s rrna was occupied by an intervening sequence (ivs) of about 100 nucleotides, analogous to ivss found in salmonellae (burgin et al., 1990). nucleotide sequences of ivss of several y. enterocolitica strains revealed that the ivss of the highly virulent y. enterocolitica serotypes strains, and the ivs of salmonella typhimurium were about 90% simila ... | 1991 | 1710756 |
| from the food and drug administration. | | 1991 | 1711593 |
| eric sequences: a novel family of repetitive elements in the genomes of escherichia coli, salmonella typhimurium and other enterobacteria. | we describe a family of highly conserved, enterobacterial repetitive intergenic consensus (eric) sequences, 14 of which have been identified in escherichia coli and salmonella typhimurium and a further three in other enterobacterial species (yersinia pseudotuberculosis, klebsiella pneumoniae and vibrio cholerae). eric sequences are 126 bp long and appear to be restricted to transcribed regions of the genome, either in intergenic regions of polycistronic operons or in untranslated regions upstrea ... | 1991 | 1713281 |
| [monospecific agglutinating sera for the identification of yersinia strains]. | an original scheme of rabbit immunization with yersinia o-antigen is suggested. technology for preparation of monospecific yersinia sera to be used in drop agglutination test on the glass has been developed. field and laboratory trials evidence that the prepared sera are fit for serologic identification of y. enterocolitica. | 1991 | 1714999 |
| a ribosomal protein of yersinia pseudotuberculosis having partial epitope identity with hla-b27. | the phenotype hla-b27 is common in patients who develop reactive arthritis after having an infection. one hypothesis concerning the pathogenesis of reactive arthritis is that molecular mimicry between hla-b27 and certain bacterial components might be involved. it is known that an infection with yersinia is commonly associated with reactive arthritis in b27 positive patients. therefore, we were interested to investigate whether cross-reactivity between yersinia and hla-b27 exists. a gene library ... | 1990 | 1715196 |
| [a method of determining glucose-6-phosphate dehydrogenase in bacteria of the genus yersinia]. | a method for qualitative assessment of glucose-6-phosphate dehydrogenase in yersinia is suggested. the reaction mixture was prepared by mixing the ingredients in the ratios as follows: 10 microliters of 0.01 m glucose-6-phosphate, 10 microliters of 0.0075 m nadp, 30 microliters of 0.75 m tris hcl ph 7.8, 10 microliters of 0.2 m mgcl2, 20 microliters of distilled water. the reaction was triggered by adding 20 microliters of cell-free bacterial extract. after 110-130 min incubation 10-20 microlite ... | 1991 | 1715948 |
| [the development of a c1q-solid-phase immunoenzyme method for determining circulating immune complexes]. | the method of quantitative enzyme immunoassay (eia) for the determination of circulating immune complexes (cic) was developed on the basis of solid-phase human c1q. the calibration curve was plotted with the use of aggregated human gamma-globulin (ahgg), the optimum range of concentration being 15-500 microg/ml. in the process of approbation on clinical material the method revealed an elevated level of cic in the sera of patients in comparison with their level in the sera of healthy donors. out ... | 1991 | 1716037 |
| mapping and topographic localization of epitopes of the yersinia pseudotuberculosis invasin protein. | the yersinia pseudotuberculosis invasin protein is a 986-amino-acid outer membrane protein that promotes bacterial penetration into mammalian cells by binding to beta 1-chain integrin receptors. we previously showed that the integrin binding domain is encoded by the carboxyl-terminal 192 amino acids. to further investigate the structure of this protein, we characterized a set of 32 monoclonal antibodies (mabs) directed against invasin. invasin deletion derivatives and fusion proteins carrying di ... | 1991 | 1716609 |
| evolution of the ferric enterobactin receptor in gram-negative bacteria. | using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of iron-deficient and replete cell envelopes, 59fe-siderophore uptake studies, and western immunoblots and cytofluorimetric analyses with monoclonal antibodies (mabs), we surveyed a panel of gram-negative bacteria to identify outer membrane proteins that are structurally related to the escherichia coli k-12 ferric enterobactin receptor, fepa. antibodies within the panel identified fepa epitopes that are conserved among the majority ... | 1991 | 1717434 |
| isolation of yersinia-specific t cell clones from the synovial membrane and synovial fluid of a patient with reactive arthritis. | synovial fluid (sf) mononuclear cells from patients with reactive arthritis (rea) proliferate in vitro when challenged with rea-associated bacteria, the maximal response being for the organism causing the triggering infection. we report the results of a study of the antigenic specificity of synovial t lymphocytes from an hla-b27 positive rea patient whose sf mononuclear cells responded preferentially to yersinia antigens. this is the first report of the isolation of yersinia-specific t cell clon ... | 1991 | 1718288 |
| the putative role of members of the cea-gene family (cea, nca an bgp) as ligands for the bacterial colonization of different human epithelial tissues. | immobilized purified cea (carcinoembryonic antigen), nca (non-specific crossreacting antigen) and bgp i (biliary glycoprotein i) bind strains of e. coli (including epec) and some salmonella species (including s. typhi, s. paratyphi a + b and s. java) while shigella-, yersinia- and bacteroides- strains showed no adhesion. the binding was of high avidity, heat sensitive, dose dependent, saturable and nearly completely abolished in the presence of 10 mm alpha-methylmannoside. from inhibition studie ... | 1991 | 1718301 |
| an extended antigenic scheme for yersinia pseudotuberculosis. | | 1991 | 1718658 |
| somatic and flagellar antigens of yersinia enterocolitica and related species. | | 1991 | 1718659 |
| rapid method for isolation and staining of bacterial lipopolysaccharide. | classically bacterial lipopolysaccharide (lps) purification and silver staining take several days. we designed a simple and fast method for lps isolation which when combined with silver staining using pharmacia phastsystem both can be completed in few hours. the purity of lps isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of lps patterns in gels. | 1991 | 1719357 |
| molecular epidemiology of yersinia enterocolitica o:3 infections: use of chromosomal dna restriction fragment length polymorphisms of rrna genes. | yersinia enterocolitica is a major enteric pathogen associated with a wide variety of clinical and immunologic manifestations, including transfusion-associated disease, from which there is a high mortality. although previously rare in the united states, in the late 1980s y. enterocolitica o:3 emerged as the predominant serotype in the united states, as it has been in canada, europe, and japan. epidemiologic investigation of this serogroup has been hampered by the limited availability of a phage ... | 1991 | 1723068 |
| microbial pathogenesis and tyrosine dephosphorylation: surprising 'bedfellows'. | the importance of parasite-directed phosphatases in such diseases as smallpox and the bubonic plague emphasizes the need to understand the molecular events associated with the normal function of protein tyrosine phosphatase in eukaryotic cells. | 1991 | 1723471 |
| surface-exposed antigenic determinants in outer membranes of wild yersinia pestis isolates. | immunogenic surface exposed envelope proteins of yersinia pestis strains were investigated with sds-page and immunoblots with antisera of immunized guinea pigs and convalescent patients. the sarkosyl-insoluble outer membrane proteins (omps) of three human isolates and one laboratory strain of y. pestis grown in different rich media to exponential or stationary phase, and cultivated at 28 degrees c or 37 degrees c, were assayed for the presence of immunogenic peptides. seven guinea-pig sera immun ... | 1991 | 1724154 |
| molecular cloning and genetic characterization of the rfb region from yersinia pseudotuberculosis serogroup iia, which determines the formation of the 3,6-dideoxyhexose abequose. | the rfb region of yersinia pseudotuberculosis serogroup iia has been cloned and expression of o antigen in escherichia coli k12 was demonstrated. transposon mutagenesis analysis confined the dna region required for o antigen expression to a 19.3 kb fragment, and the o antigen expressed was visualized by sds-page and silver staining. southern hybridization analysis demonstrated significant levels of similarity between the yersinia rfb region and the 3,6-dideoxyhexose pathway genes rfbf and rfbg, ... | 1991 | 1724263 |
| mechanism of yada-mediated serum resistance of yersinia enterocolitica serotype o3. | complement activation via the alternative pathway was analyzed with isogenic strains of yersinia enterocolitica serotype o3 differing in plasmid content (p- or p+ strains) or selective lack of yada expression (yada- strain). the p+ strain was serum resistant, even after antibody-enhanced complement activation. serum sensitivity was observed with the p- and yada- strains but was more pronounced in the p- strain. the p+ strain deposited less c5b-9(m) complexes on its surface than the p- and yada- ... | 1992 | 1729182 |
| the salmonella typhimurium virulence plasmid complement resistance gene rck is homologous to a family of virulence-related outer membrane protein genes, including pagc and ail. | a fragment of the salmonella typhimurium virulence plasmid containing the rck locus, when cloned in the recombinant cosmid pade016, was shown previously to confer high-level complement resistance on both rough and smooth escherichia coli, salmonella minnesota, and s. typhimurium and was associated with the production of an outer membrane protein. we determined the nucleotide sequence of the fragment containing the rck locus. mutations in the two major open reading frames confirmed that the compl ... | 1992 | 1729227 |
| pneumonia. patient profiles, choice of empiric therapy, and the place of third-generation cephalosporins. | choosing appropriate antimicrobial therapy for patients with pneumonia requires knowledge of the etiologic agents seen in specific kinds of patients at specific times and places. for community-acquired pneumonia, there is an important difference in the agents seen in the normal and the compromised host. the normal host most often presents with viral, mycoplasmal, or pneumococcal pneumonia. the exact place of chlamydia pneumoniae is still under study. a normal host who aspirates is at risk of ana ... | 1992 | 1730186 |
| human platelet aggregation by yersinia pseudotuberculosis is mediated by invasin. | plasmid-free strains of yersinia pseudotuberculosis induce aggregation of human platelets in vitro. it appears that this phenomenon is mediated by invasin (inv), a 103-kda outer membrane protein that permits bacteria to penetrate mammalian cells, since (i) an isogenic inv-deficient mutant failed to aggregate platelets compared with the parental strain; (ii) a monoclonal antibody directed against invasin inhibited platelet aggregation; (iii) inv+ escherichia coli hb101 promoted platelet aggregati ... | 1992 | 1730468 |
| binding of cultured mammalian cells to immobilized bacteria. | the invasin protein of yersinia pseudotuberculosis binds to integrin receptors on mammalian cells and promotes cellular penetration. we demonstrate here that the cell attachment activity of invasin can be detected in bacterial colonies that have been immobilized on filter membranes. invasin expressed in either escherichia coli k-12 or y. pseudotuberculosis mediated binding to membranes, and as few as 10(5) y. pseudotuberculosis resulted in detectable attachment of cultured epithelial cells. a si ... | 1992 | 1730504 |
| the invasin protein of yersinia enterocolitica: internalization of invasin-bearing bacteria by eukaryotic cells is associated with reorganization of the cytoskeleton. | yersinia enterocolitica, a facultative intracellular pathogen of mammals, readily enters (i.e., invades) cultured eukaryotic cells, a process that can be conferred by the cloned inv locus of the species. we have studied the mechanism by which the product of inv, a microbial outer membrane protein termed "invasin," mediates the internalization of bacteria by hep-2 cells and chicken embryo fibroblasts. invasin-bearing bacteria initially bound the filopodia and the leading edges of cultured cells. ... | 1992 | 1730744 |
| catecholamine induced growth of gram negative bacteria. | the addition of various catecholamines to cultures of gram negative bacteria resulted in dramatic increases in growth. the ability of norepinephrine, epinephrine, dopamine and dopa to enhance the growth of escherichia coli, yersinia enterocolitica and pseudomonas aeruginosa was observed to be dependent on the bacterium employed with each strain showing marked preference for one or more of the catecholamines. catecholamine induced increases in growth were confirmed by one or more of the following ... | 1992 | 1731173 |
| potential interpretational errors in the use of the polymerase chain reaction to detect yersinia in clinical specimens. | | 1992 | 1731811 |
| a lipoprotein of yersinia enterocolitica facilitates ferrioxamine uptake in escherichia coli. | a cloned fragment of yersinia enterocolitica dna complemented the defect in ferrioxamine b uptake of an escherichia coli fhue mutant lacking the outer membrane high-affinity transport protein fhue. subcloning revealed that a 13.7-kda outer membrane protein was required for complementation. the amino acid sequence deduced from the nucleotide sequence showed extensive homology to pcphi, an outer membrane lipoprotein of haemophilus influenzae. we therefore termed this protein pcpye. plasmid-encoded ... | 1992 | 1732192 |
| characterization of the caulobacter crescentus flbf promoter and identification of the inferred flbf product as a homolog of the lcrd protein from a yersinia enterocolitica virulence plasmid. | we have investigated the organization and expression of the caulobacter crescentus flbf gene because it occupies a high level in the flagellar gene regulatory hierarchy. the nucleotide sequence comprising the 3' end of the flao operon and the adjacent flbf promoter and structural gene was determined, and the organization of transcription units within this sequence was investigated. we located the 3' ends of the flao operon transcript by using a nuclease s1 protection assay, and the 5' end of the ... | 1992 | 1732219 |
| the expression of virulence genes in listeria monocytogenes is thermoregulated. | the expression of listeriolysin, a major virulence factor of the gram-positive facultative intracellular pathogen listeria monocytogenes, is positively regulated by a transcriptional activator, the prfa gene product. we had previously shown that mutations within the prfa gene lead to loss of listeriolysin production. in this communication, the regulation of expression of listeriolysin by a specific environmental condition, namely, temperature, was studied in wild-type strains of listeria monocyt ... | 1992 | 1732227 |
| isolation of yersinia enterocolitica serovar o8 from free-living small rodents in japan. | yersinia species were isolated from 65 of 223 free-living small mammals trapped in 10 regions on honshu island in japan. of the 65 strains isolated, 1 was yersinia enterocolitica serovar o3, 8 were y. enterocolitica o5, 6 were y. enterocolitica o8, 3 were y. enterocolitica o9, and 1 was yersinia pseudotuberculosis 4b. of the six y. enterocolitica o8 strains, five were positive for autoagglutination, ca2+ dependence, and the 45-mda virulence plasmid and showed high pathogenicity for mice. | 1992 | 1734061 |
| mucosal cell-mediated immunity to mycobacterial, enterobacterial and other microbial antigens in inflammatory bowel disease. | culture studies have suggested that mycobacterium paratuberculosis may play a role in the aetiology of crohn's disease. however, evidence of sensitization to mycobacterial antigens amongst patients with crohn's disease has not yet been adequately demonstrated. previous studies of cell-mediated immunity (cmi) in crohn's disease were restricted to responses of peripheral blood mononuclear cells (pbmc) to mycobacterial antigens. in this study we have investigated the proliferative responses of both ... | 1992 | 1735186 |
| [clinical findings and diagnosis of yersinia enterocolitica infections; a retrospective study in friesland]. | | 1992 | 1736136 |
| serum bactericidal activity for yersinia enterocolitica in hemodialysis patients: effects of iron overload and deferoxamine. | human serum has been shown to be bactericidal for most strains of yersinia enterocolitica. systemic y enterocolitica infections have been reported in iron-overloaded hemodialysis patients treated with deferoxamine. both iron and deferoxamine are known to enhance the growth of y enterocolitica. we inoculated sera from 12 hemodialysis patients whose serum ferritin levels ranged from 26 to 6,855 micrograms/ml (ng/ml), as well as three controls, with yersinia organisms. after latencies of 0 to 24 ho ... | 1992 | 1739096 |
| [molecular and cellular bases of shigella flexneri virulence]. | shigella flexneri, a gram negative bacillus, causes bacillary dysentery, an ulcerative disease of the human colon, by invading intestinal epithelial cells. entry into epithelial cells occurs via an induced phagocytic process which involves the actino-myosin complex. the host-cell receptor and the transmembrane signal which initiate reorganization of the cytoskeleton are under study. binding to integrins has recently been demonstrated in related models such as the entry of yersinia pseudotubercul ... | 1991 | 1742620 |
| etiology and management of diarrhoeal diseases in karachi. | the incidence of diarrhoeal disease are very high in our population and bacterial etiology amounts to 43.55% of all cases. pathogen such as aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica and campylobacter are also present in significant numbers, they make up 50% of bacterial causes. all enteric pathogens can be easily isolated on commonly used media and could be precisely identified by simple biochemical tests. | 1991 | 1744967 |
| relationship between clinical and milk isolates of yersinia enterocolitica. | in the summer of 1987-1988, an outbreak of 11 cases of yersinia enterocolitica enteritis caused by 2 serogroups (0:3, 0:6,30) occurred prompting an investigation into possible environmental sources. symptoms were present for a mean of 9 days and occurred in 2 distinct age groups--toddlers (7) who presented with diarrhea, and young adults (4), 3 of whom presented clinically with appendicitis. in a survey of 39 randomly chosen pasteurized milk samples, 9 were positive for growth of y. enterocoliti ... | 1991 | 1745567 |
| the role of yersinia enterocolitica in appendicitis in the southwestern united states. | infection with yersinia enterocolitica had been associated with acute appendicitis in approximately six per cent of patients in northern european countries. however, the incidence of y. enterocolitica in patients with appendicitis in this country is uncertain. therefore, this study was undertaken to ascertain whether y. enterocolitica is a possible infectious agent in appendicitis in the southwestern united states. fifty prospective patients (35 men and 15 women) with an average age of 22.3 year ... | 1991 | 1746791 |
| [isolation of yersinia, campylobacter, plesiomonas and aeromonas from environmental water and fresh water fishes]. | yersinia, campylobacter, plesiomonas and aeromonas are known causative agents in waterborne diseases. for about 10 years, outbreak of diarrhea has been observed, especially among children, in the mountain areas of okayama. y. pseudotuberculosis recently isolated from non-chlorinated drinking water sources such as mountain streams and wells has been suspected to be the causative bacteria of the disease. attempts were made to isolate yersinia and campylobacter from water samples from rivers in rur ... | 1991 | 1747520 |
| molecular mimickry between hla b27 and yersinia, salmonella, shigella and klebsiella within the same region of hla alpha 1-helix. | two new examples of amino acid homology between hla b27 and microbes triggering hla b27-associated diseases are described. an outer membrane protein yada (yersinia adhesin, previously called yop1) of yersinia enterocolitica and y. pseudotuberculosis shares a linear tetrapeptide with hla b27. a cationic outer membrane protein omph of salmonella typhimurium shares homology with five amino acids of hla b27 in a non-linear fashion. the four amino acids of yada are also notably included in the hexape ... | 1991 | 1747948 |
| clinical, clinicopathologic, and pathologic features of plague in cats: 119 cases (1977-1988). | the clinical, clinicopathologic, and pathologic features of 119 cases of plague in cats from 1977 to 1988 in new mexico were reviewed. fifty-three percent were bubonic, 10% were pneumonic, 8% were septicemic, and 29% with neither buboes nor pneumonia were unclassified (but presumed septicemic). three quarters of the lymphadenopathy was submandibular, and almost half of this was bilateral. one third of all cats had the triad of lethargy, anorexia, and fever in addition to buboes; one quarter had ... | 1991 | 1752774 |
| susceptibility of wild mice to yersinia pseudotuberculosis and yersinia enterocolitica. | laboratory-born progeny and adult populations of old world woods mice (apodemus speciosus) trapped in a certain area where yersinia pseudotuberculosis was prevalent among wild animals, were challenged with y. pseudotuberculosis and yersinia enterocolitica to determine the ecology of yersinia in wild mice. the laboratory-born progeny population was associated with infection with y. pseudotuberculosis but not with y. enterocolitica serotype o:3 and was found to be nearly 200 times more susceptible ... | 1991 | 1755925 |
| the nucleotide and deduced amino acid sequence of the cationic 19 kda outer membrane protein omph of yersinia pseudotuberculosis. | the omph proteins of enteric bacteria are recently described, small (16 kda), cationic outer membrane proteins. because a yersinia pseudotuberculosis cell envelope protein of this size has been found to cross-react serologically with the human histocompatibility antigen hla-b27 (b*2701), the sequence of y. pseudotuberculosis omph was determined by sequencing the gene region which encodes mature omph. a protein consisting of 143 amino acid residues was found. it was 96% homologous with the omph o ... | 1991 | 1756172 |
| a wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaa gene of yersinia enterocolitica. | a new suicide vector (pkng101) that facilitates the positive selection of double recombination events in gram-bacteria has been developed. it contains a conditional origin of replication (orir6k), the strab genes encoding the streptomycin phosphotransferase (smr), an origin of transfer (mobrk2), the sacb gene mediating sucrose sensitivity, and multiple cloning sites. it was used to mutate the blaa gene of yersinia enterocolitica, by marker-exchange mutagenesis. to do this, we have first cloned i ... | 1991 | 1756974 |
| [bilateral chorioretinitis after infection with yersinia enterocolitica]. | we report a case of severe, bilateral panuveitis with disseminated chorioretinis. with systemic steroid therapy the inflammation resolved within 4 weeks, resulting in chorioretinal scarring. because the agglutination titer against yersinia enterocolitica type 3 was increased to 6 times over normal, we consider yersinia to be the most probable cause of the panuveitis. concomitantly, serology revealed evidence for recent infection with mumps and shigella. both can cause anterior uveitis. therefore ... | 1991 | 1757029 |
| yersinia enterocolitica isolated from two cohorts of young children in santiago, chile: incidence of and lack of correlation between illness and proposed virulence factors. | yersinia enterocolitica was isolated from children in two cohorts in santiago, chile. in a cohort containing a cross section of children aged 0 to 4 years, y. enterocolitica was isolated from stool samples of 1.1% of children with diarrhea and 0.2% of age-matched control children. in a subgroup of this cohort from which weekly stool samples were obtained from all children irrespective of clinical status, 6% of children had asymptomatic yersinia infections. in a birth cohort (with a greater repre ... | 1991 | 1757549 |
| yersinia enterocolitica: a frequent seasonal stool isolate from children at an urban hospital in the southeast united states. | from 1 december 1988 through 28 february 1991, 7,290 rectal swab specimens received in our laboratory were screened for yersinia enterocolitica. a total of 76 patients had y. enterocolitica isolated from their stool samples. of these patients, 59 (77.6%) were 12 months old or younger. y. enterocolitica was second only to salmonella spp. in this age group. routine screening for y. enterocolitica may be warranted in hospitals serving large pediatric populations. | 1991 | 1757561 |
| yersiniosis in free-ranging muskoxen on banks island, northwest territories, canada. | sixty-seven muskox (ovibos moschatus) carcasses, 53 skeletal remains and two sick muskoxen were seen during an aerial survey of the thomsen river region, northern banks island, northwest territories, canada in late july 1986. complete necropsies were performed on 29 muskoxen estimated to have died within the previous 3 to 5 days. twenty were diagnosed with acute yersiniosis due to yersinia pseudotuberculosis serotype 1b and were in excellent body condition. a diagnosis could not be made on seven ... | 1991 | 1758017 |
| [design of a species-specific dna probe based on the pfra plasmid of the plague pathogen]. | the recombinant plasmid pbs1 carrying a 2 kb salgi fragment of yersinia pestis pfra plasmid was constructed by insertion of the fragment into a vector plasmid pbr327. salgi-bspri 400 bp subfragment was recloned into a pbr322 vector plasmid. open reading frame was found in the fragment by dna sequencing technique. the subfragment designated f1-probe permits one to identify specifically the yersinia pestis strains harbouring pfra plasmid, thus, differing them from closely related yersiniea and oth ... | 1991 | 1758469 |
| [the cultural properties and virulence of yersinia]. | study of the cultivation properties of 82 enterobacterial strains has revealed that the colonies of virulent y. enterocolitica (serovars o3, o9) and y. pseudotuberculosis (serovar i) are temperature-sensitive. this sign, closely connected with the presence and expression of the virulence plasmid with a molecular weight of 44-48 md, is not characteristic of other strains. virulent yersinia grown in nutrient agar for 48 hours at 37 degrees c form colonies which are smaller in diameter than those f ... | 1991 | 1759512 |
| [an experimental study of yersinia in plants]. | y. enterocolitica and y. pseudotuberculosis have been experimentally found to penetrate into plants (cabbage, salad, pea, oat) from infected soil and water and to remain in different plant organs (roots, seeds, leaves) up to 30 days (the term of observation). the processes of s-r dissociation in yersinia cultures isolated from plants, as well as changes in some enzymatic properties, have been established. in the test on infusoria the cytotoxic action of strains isolated from plants has been note ... | 1991 | 1759521 |
| gastroenterological emergencies in the tropics. | significant differences exist in the prevalence of most gastroenterological emergencies in tropical compared with temperate countries. both ethnic and environmental (often clearly defined geographically) factors are relevant. the major oesophageal lesions which can present acutely in tropical countries are varices and carcinoma; bleeding and obstruction are important sequelae. peptic ulcer disease (and its complications), often associated (not necessarily causally) with helicobacter pylori infec ... | 1991 | 1764626 |
| a survey of acute and chronic disease associated with yersinia enterocolitica infection. a norwegian 10-year follow-up study on 458 hospitalized patients. | the aim of the present study was to further elucidate acute and chronic manifestations of yersinia enterocolitica infection. during the period 1974-83, 458 hospitalized patients were diagnosed by antibody response and/or isolation of the microorganism. 64 patients had suffered from chronic conditions as rheumatic disease, inflammatory bowel disease, hepatitis, nephritis or thyroid disease for some time. acute hepatic, renal, cardiac, pulmonary, pancreatic or neurologic involvement were observed ... | 1991 | 1767249 |
| biotype and antibiotic sensitivity of 100 clinical isolates of yersinia enterocolitica. | one hundred clinical isolates of yersinia enterocolitica were biotyped, serotyped and tested for in-vitro sensitivity to seven beta-lactam antibiotics and six other antibiotics. twenty-four of the isolates belonged to niléhn's and wauters' biotype 1a, 12 to biotype 3 and 64 to biotype 4. no biotypes 1b or 2 strains were found. all isolates were sensitive to chloramphenicol, ciprofloxacin, gentamicin, tetracycline, trimethoprim and all but one to sulphafurazole. all were resistant to ampicillin w ... | 1991 | 1769931 |
| a study of the beta-lactamases of 100 clinical isolates of yersinia enterocolitica. | the intracellular beta-lactamases of 100 distinct clinical isolates of yersinia enterocolitica were examined, to investigate the effect of selected beta-lactam agents on the enzymes. the enzyme inhibition profiles obtained were specific to each of the three biotypes 1a, 3 and 4. biotype 4 strains elaborated only one beta-lactamase with a pi of 8.3 corresponding to the carbenicillin-hydrolysing beta-lactamase enzyme a of cornelis & abraham. biotype 3 strains also produced a single beta-lactamase ... | 1991 | 1769940 |
| virulence and serum-resistance in different clonal groups and serotypes of yersinia ruckeri. | the virulence in rainbow trout (oncorhynchus mykiss) of 32 isolates of yersinia ruckeri, representing a range of biotypes, serotypes, and omp-types, was examined. virulence was assayed in fish of average weight 7.7 g by bath challenge for 1 h with approximately 5 x 10(7) cells per ml. two of the six serotype o1 clonal groups of y. ruckeri, clones 2 and 5, were virulent, whereas the other four clonal groups, clones 1, 3, 4 and 6, as well as all serotype o2, o5, o6 and o7 isolates examined, were a ... | 1991 | 1771752 |
| [microbiologic findings in protracted and chronic enterocolitis]. | novel diagnostic methods (immunoblotting, immunofluorescence test) have been proved to be very sensitive and specific for detecting enteropathogenic bacilli. 28 patients with chronic enterocolitis were investigated. in 50% (14/28) of these patients yersinia enterocolitica could be visualized in biopsies by immunofluorescence. the serum of 8 patients also contained iga-antibodies against plasmid encoded released proteins of y. enterocolitica. this paper reports evidence for persistence of yersini ... | 1991 | 1771939 |
| determination of oxygen radical production in spondyloarthropathies by whole blood chemiluminescence. | oxygen derived free radicals are considered to play an important part in the development of inflammation. a whole blood chemiluminescence assay was used to study n-formyl-methionyl-leucyl-phenylalanine induced oxygen radical production in subjects with ankylosing spondylitis or previous yersinia arthritis. in luminol enhanced chemiluminescence, the subjects with previous yersinia arthritis showed significantly increased initial activation (at one minute), whereas the subjects with ankylosing spo ... | 1991 | 1772293 |
| [bacteriologic monitoring of 1,000 units of human packed red blood cells for yersinia enterocolitica]. | several reports of yersinia enterocolitica (ye) post-transfusion septicaemia have been recently published. in order to study the prevalence of ye contamination of packed red blood cells (prbc) we performed a post-transfusion control on 1,000 prbc units. we did not find any ye contamination. this is in accordance with the rarity of the reported cases. however as they are very serious, a prevention is necessary. as classical bacteriological techniques are not feasible, the only possibility is to d ... | 1991 | 1772523 |
| isolation of yersinia enterocolitica serogroup o:8, biogroup 1b. | | 1991 | 1774289 |
| [structure of the o-specific polysaccharide chain of yersinia aldovae lipopolysaccharide]. | o-specific polysaccharide has been isolated on mild hydrolysis of lipopolysaccharide from yersinia aldovae and shown to consist of 2-acetamido-2-deoxy-d-glucose, d-glucose, 2-acetamido-2-deoxy-d-galactose, and 3,6-dideoxy-3- [(r)-3-hydroxybutyramido]-d-galactose in molar ratio 2:2:1:1. acid hydrolysis, methylation, solvolysis with anhydrous hydrogen fluoride, 1h and 13c nmr studies indicated the polysaccharide to be composed of hexasaccharide repeating units of the following structure: [formula ... | 1991 | 1776968 |
| occurrence of yersinia spp. in foods in brazil. | over the past 9 years, 468 bacterial strains isolated from raw and pasteurized milk, beef and pork, bovine and chicken liver, chicken heart, gizzards and lung sausage, hamburger, cheese and lettuce in different regions of the state of são paulo and in the city of rio de janeiro were received by the reference laboratory for yersinia in brazil. all were confirmed to be yersinia spp. the 468 yersinia isolates were grouped as 184 strains because some of the bacteria isolated from the same food sampl ... | 1991 | 1777388 |
| survival and injury of escherichia coli, campylobacter jejuni, and yersinia enterocolitica in stream water. | experiments were done to describe the survival and injury of three strains each of enteropathogenic escherichia coli, yersinia enterocolitica, and campylobacter jejuni in agricultural surface water. bacterial suspensions within membrane diffusion chambers were immersed in two large vessels of stream water that were held at 6 and 16 degrees c and changed daily. the results of daily plate counts, using selective and nonselective media, revealed some variation among strains and genera. injury incre ... | 1991 | 1777856 |
| [indicators of oxygen-dependent metabolism of peripheral blood neutrophils in the differential diagnosis of bacterial and viral infections]. | oxygen-dependent metabolism of peripheral neutrophils was compared for viral hepatitis and acute yersiniosis patients. the metabolism inhibition was seen in both the diseases with lower values for viral hepatitis. neutrophil function was dependent on the disease gravity. its recovery went in parallel with other clinical and laboratory signs of convalescence. a luminol-dependent chemiluminescence test is proposed for differential diagnosis of viral hepatitis with jaundices in generalized bacteria ... | 1991 | 1780777 |
| yersinia in effluents from the food-processing industry. | yersinia enterocolitica and yersinia pseudotuberculosis are current sources of pathogenic strains in humans and animals. yersiniae infections occur throughout the world, but are most prevalent in regions with moderate and subtropical climates. in australia, central europe and north america, cases of human infections with yersinia enterocolitica now rank in third place. the food-processing industry may influence the epidemiological situation in different ways. effluents which contaminate the envi ... | 1991 | 1782430 |
| recognition by peptide mapping of three different structural groups of outer membrane protein yop-1 of yersinia enterocolitica and yersinia pseudotuberculosis. | the structural relation of yop-1 of "european" and "american" yersinia enterocolitica serotypes o:3, o:9, o:5,27, and o:8 and o:20, respectively, and y. pseudotuberculosis serotypes i, ii, and iii was compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis and peptide mapping using staphylococcus aureus protease v8. apparent molecular weights of yop-1 ranged from 206,000 (o:3) to approx. 180,000 (o:8). according to their respective peptide maps yop-1 of the "european" and "american ... | 1991 | 1784270 |
| [protein expression of yersinia pestis during changes due to long-term cultivation in vivo]. | the electrophoretic study of yersinia pestis proteins made possible to find the significant modification of yersinia pestis polypeptide specters when the bacteria were cultivated in semi-penetrable cells implanted into the guinea pigs peritoneum. the proteinogramms of the isolates from the implanted cells lacked the stained bands characteristic of yersinia pestis cells grown in vitro and contained the new polypeptides absent from the bacteria grown on the hottinger agar plates. the difference wa ... | 1991 | 1784300 |
| [yersinia enterocolitica bacteremia and hemochromatosis]. | | 1991 | 1784788 |
| [cloning and detailed mapping of the fra-ymt region of the yersinia pestis pfra plasmid]. | the genetical libraries of the pfra plasmid of yersinia pestis genes were obtained by insertion into the psti, salgi, ecori, xhoi restriction sites of the cosmid vector phc79. the immunochemical analysis of the recombinant clones has revealed the clones synthesizing the antigen fl (fraction i) and mouse toxin (ymt--yersinia pestis murine toxin). the restriction analysis of the plasmids from antigen synthesizing clones has permitted to construct the detailed physical map of the fra-ymt region of ... | 1991 | 1787840 |
| [the role of a plasmid with a molecular weight of 82 md in the modulation of the action of yersinia pseudotuberculosis on the proliferative activity of murine lymphoid cells]. | | 1991 | 1787842 |
| [the gene identification of bacterial species and serovariants by the polymerase chain reaction with universal oligonucleotides: the reidentification of earlier isolated strains of yersinia pseudotuberculosis]. | genetic analysis of 19 standard strains belonging to 6 yersinia species (y. pestis, y. pseudotuberculosis, y. enterocolitica, y. kirstensenii, y. frederiksenii, y. intermedia) revealed that gene typing by the method of polymerase chain reaction (pcr) with the use of universal primers permitted the identification of species in bacterial cultures by pcr patterns and the determination of y. pseudotuberculosis serovars within 4 hours. by this method 23 y. pseudotuberculosis strains (serovar 1), earl ... | 1991 | 1789029 |
| microinjection of the yersinia yope cytotoxin in mammalian cells induces actin microfilament disruption. | | 1991 | 1794475 |