| biosynthesis and catabolism of 6-deoxy l-talitol by klebsiella aerogenes mutants. | a mutant strain of klebsiella aerogenes was constructed and, when incubated anaerobically with l-fucose and glycerol, synthesized and excreted a novel methyl pentitol, 6-deoxy l-talitol. the mutant was constitutive for the synthesis of l-fucose isomerase but unable to synthesize l-fuculokinase activity. thus, it could convert the l-fucose to l-fuculose but was incapable of phosphorylating l-fuculose to l-fuculose 1-phosphate. the mutant was also constitutive for the synthesis of ribitol dehydrog ... | 1980 | 6988406 |
| [biosynthesis of iron-binding metabolites by klebsiella aerogenes]. | | 1980 | 6988484 |
| glucose phosphoenolpyruvate phosphotransferase activity and glucose uptake rate of klebsiella aerogenes growing in chemostat culture. | glucose-limited cultures of klebsiella aerogenes nctc 418 (and the supposedly identical strain ncib 418) possessed a glucose phosphoenolpyruvate (pep) phosphotransferase activity that varied markedly and progressively with growth rate, from more than 250 nmol min-1 (mg dry wt cells)-1 at d = 0.1 h-1 to less than 100 nmol min-1 (mg dry wt cells)-1 at d = 0.8 h-1. when relieved of the glucose limitation, substrate was used at a rate that bore no precise relationship to the cells' phosphotransferas ... | 1980 | 6989955 |
| labelling of the cyclitol permease in klebsiella aerogenes. | the protein component(s) of the cyclitol-transport system in klebsiella aerogenes has been labelled by using three different procedures. one method is based on differential alkylation with n-ethylmaleimide, and another on incorporation of amino acids during the induction process. a protein of mol.wt. 34 000 was labelled by both procedures; by alkylation with n-ethylmaleimide two other proteins of mol.wts. 55 000 and 67 000 were also labelled. the third uses diazotized [35s]sulphanilic acid after ... | 1980 | 6990918 |
| frequency of faecal klebsiella aerogenes in patients with ankylosing spondylitis and controls with respect to individual features of the disease. | a study of 59 patients with definite ankylosing spondylitis and 41 comparable hospital outpatients with fractures has been undertaken to determine if the presence of faecal klebsiella aerogenes is related to clinical activity of the spinal disease and its extraspinal features. the frequencies of fecal k. aerogenes were similar in both patients and controls and were not significantly related to spinal disease activity. careful inquiry about antibiotic treatment, dietary habits, and hospitalisatio ... | 1980 | 6992718 |
| influence of growth environment on the phosphoenolpyruvate: glucose phosphotransferase activities of escherichia coli and klebsiella aerogenes: a comparative study. | a consistent difference was found between glucose-limited cultures of escherichia coli and klebsiella aerogenes strains in the manner which their apparent cellular content of glucose:phosphoenolpyruvate phosphotransferase (glucose-pts) varied with growth rate. with the former strains, activity increased as a function of growth rate; in the latter it decreased. however, under glucose-sufficient conditions (potassium- or ammonia-limitation) both species behaved similarly; the glucose-pts activity ... | 1980 | 6992732 |
| [occurrence and importance of klebsiella aerogenes as a hospital germ]. | | 1980 | 6998145 |
| evaluation of the four-hour micro-id technique for direct identification of oxidase-negative, gram-negative rods from blood cultures. | a 4-h micro-id technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by api 20e and micro-id, using standardized inocula. a total of 127 patients (220 positive cultures) were studied. isolates included 96 escherichia coli, 46 klebsiella pneumoniae, 7 klebsiella oxytoca, 8 enterobacter aerogenes, 17 enterobacter cloacae, 19 serratia marcescens, 2 serratia liquefaciens, 8 prot ... | 1980 | 6999020 |
| distinctive electrophoretic patterns of esterases from klebsiella pneumoniae, k. oxytoca, enterobacter aerogenes and e. gergoviae. | esterases of 14 strains of klebsiella pneumoniae, 14 strains of k. oxytoca, 16 strains of enterobacter aerogenes and 16 strains of e. gergoviae were analysed by horizontal electrophoresis in polyacrylamide-agarose gel. four principal esterase bands (designated e1 to e4) and nine minor bands differing in their activity towards synthetic substrates and in their sensitivity to heat and to di-isofluoropropyl phosphate were defined. the comparative distribution of bands showed that the four species a ... | 1980 | 6999117 |
| minicircular cole1-related dna in strains of klebsiella aerogenes selected for fast growth on xylitol. | we have previously described a large family of mutants of klebsiella aerogene which were selected by continuous on xylitol and which superproduce ribitol dehydrogenase. one of these strains was found to harbour a high copy number 2.1 x 10(6) dalton plasmid. this plasmid is a deletion derivative of a low copy number 3.5 x 10(6) dalton plasmid present in the ancestral strain of k. aerogenes. however, since these plasmids do not contain the genes required for pentitol catabolism and some enzyme-sup ... | 1980 | 6999122 |
| immunoferrin labeling of respiratory nitrate reductase in membrane vesicles of bacillus licheniformis and klebsiella aerogenes. | the indirect immunoferritin labeling method was used to localize the membrane-bound respiratory nitrate reductase in membrane vesicles and protoplasts or sphereplasts of bacillus licheniformis and klebsiella aerogenes, respectively. for a comparison of the labeling of the various vesicle preparations, which differed not only in size but also in the percentage of inside-out orientation, a quantification of the results was needed to circumvent the problem of non-specifically bound ferritin. from t ... | 1980 | 7000023 |
| [diagnosis of fecal coliforms in dairy products by the temperature tests]. | the study concerned the formation of gas and acid in broth with lactose and glucose and brilliant green at 30,37 and 44 degrees c and of indole in peptone water at 30, 37 and 44 degrees c by 842 coliform strains isolated from 386 samples of dairy products (e. coli i--496, e. coli--51, citrob. freundi--87, citrob. intermedium--46, enterobacter aerogenes--98, enterobacter cloacae -- 32 and k1. aerogenes 32). it was found that eijkman's test (formation of gas and acid from lactose in broth with bri ... | 1980 | 7001729 |
| antigenic relationships between pore proteins of escherichia coli k12. | antisera were raised against the purified escherichia coli k12 outer membrane proteins ompa-, ompc- and ompf proteins and protein e. several immunological methods were used to investigate the specificity of the antisera and the immunological relationship between the major outer membrane proteins. although the antisera had been raised against highly purified proteins, several of them contained activity against lipopolysaccharide and lipoprotein due to minor impurities in the immunogens. the three ... | 1980 | 7002546 |
| a switch from translational control to transcriptional control of protein synthesis in mid-exponential growth phase of bacterial cultures. specific radioimmune labelling of ribitol-dehydrogenase-synthesising polysomes from klebsiella aerogenes in the presence of heparin. | 1. we present evidence suggesting a sudden switch from translational control to transcriptional control of protein synthesis in mid-exponential growth of bacterial batch cultures. at a critical cell density a switch from large to small polysomes occurs during a short period of exponential growth. the profile of specific polysomes engaged in synthesis of a constitutive enzyme, ribitol dehydrogenase, changes at the same point but in an opposite way: a linear profile peaking at monosomes changes to ... | 1980 | 7002553 |
| purification and properties of aromatic amino acid aminotransferase from klebsiella aerogenes. | we describe the complete purification of aromatic aminotransferase i, the enzyme responsible for the ability of klebsiella aerogenes to use tryptophan and phenylalanine as sole sources of nitrogen, as well as the partial purification of aromatic aminotransferase iv. an examination of the properties of these enzymes revealed that aminotransferase i had much greater affinity for the aromatic amino acids than aminotransferase iv, explaining the essential role of aminotransferase i in the utilizatio ... | 1981 | 7007315 |
| studies on the induction and turnover of citrate-oxidizing capacity in klebsiella aerogenes using chemostat culture. | steady-state chemostat cultures of klebsiella aerogenes growing on a carbon-limited medium were challenged with pulse of carbon sources, and the resultant changes in dissolved oxygen tension were shown to reflect changes in respiration rate. these changes were used to study the kinetics of induction of citrate-oxidizing capacity, which is most probably limited by citrate permease, and the return to the preinduced state. previously unexposed cells showed a lag phase, the duration of which decreas ... | 1980 | 7014774 |
| characterization of a membrane-bound biotin-containing enzyme: oxaloacetate decarboxylase from klebsiella aerogenes. | oxaloacetate decarboxylase from klebsiella aerogenes is firmly bound to the cytoplasmic membrane, from which it can be solubilized with nonionic detergents. the solubilized enzyme behaved like the membrane-bound enzyme with respect to its inhibition by avidin and to the requirement of sodium ions for catalytic activity. the decarboxylase was purified 4.5-fold over the solubilized membrane extract by conventional means. dodecyl-sulfate disc-gel electrophoretic analysis indicated that the enzyme c ... | 1981 | 7016536 |
| effect of solar radiation and predacious microorganisms on survival of fecal and other bacteria. | the effect of solar radiation and predacious microorganisms on the survival of bacteria of fecal and plant origin was studied. the decline in the numbers of escherichia coli cells in estuarine water samples was found to be significantly greater in the presence of both naturally occurring microbial predators and solar radiation than when each of these factors was acting independently. the effect of solar radiation on microbial predators was negligible, whereas the susceptibility of bacteria to li ... | 1981 | 7020590 |
| microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salt/glucose media. 2. enthalpy of aerobic growth. | power-time traces for cells of k. aerogenes growing aerobically in batch culture with glucose limitation are reported and the results discussed with the corresponding mass balance data. enthalpy values during the consumption of glucose and production of cellular material are calculated during growth in glucose (concentration range 0.56-5.4 mmol dm-3). the efficiency of caloric conversion and energy requirements during growth are considered by two methods. the measured enthalpy (delta hgluc) is s ... | 1980 | 7022143 |
| multiple antibiotic resistance and capsular types of gentamicin-resistant klebsiella aerogenes. | | 1981 | 7024241 |
| gentamicin-resistant klebsiella aerogenes as a clinically-significant source of transferable antibiotic resistance. | | 1981 | 7024243 |
| isolation of iiiglc of the phosphoenolpyruvate-dependent glucose phosphotransferase system of salmonella typhimurium. | we report a procedure for the isolation of iiiglc of salmonella typhimurium, a protein component of the phosphoenolpyruvate-dependent sugar phosphotransferase system. iiiglc is a soluble protein with a molecular weight of 21,000, as determined by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the purified protein is involved in the phosphoenolpyruvate-dependent phosphorylation of methyl alpha-glucoside in vitro. its affinity for octyl-sepharose may be an indication ... | 1981 | 7026533 |
| microcalorimetric studies of klebsiella aerogenes grown in chemostat culture. 1 glucose-limited cultures. | the heat evolved during the aerobic growth of cells of k. aerogenes in continuous culture in carbon-limiting medium has been measured with a flow-microcalorimeter. problems associated with aeration, pump rates, temperature, etc, have been eliminated. the measured heat output depends on the time taken for the cells to reach the calorimeter from the chemostat, from such results the rate of heat production in the chemostat can be calculated. although the measured power output is independent of the ... | 1980 | 7026984 |
| coliform inhibition by bacteriocin-like substances in drinking water distribution systems. | bacterial isolates from an unchlorinated potable groundwater system and a chlorinated surface water system were screened by an agar overlay method for the ability to produce bacteriocin-like substances (bls) inhibitory to the growth of escherichia coli, klebsiella sp., and enterobacter aerogenes. the production of coliform-specific bls by noncoliform bacteria varied with the site and date of isolation as well as the genus of the producer strain. a total of 448 bacterial isolates were screened fr ... | 1981 | 7027953 |
| reconstitution of sodium transport from purified oxaloacetate decarboxylase and phospholipid vesicles. | the na+-pumping oxaloacetate decarboxylase, isolated from klebsiella aerogenes membranes, was incorporated into the membranes of lecithin liposomes. reconstitution of na+ transport in this artificial system was achieved by the detergent dilution method with octylglucoside as the detergent. an optimum na+ transport activity upon dilution was obtained in the presence of about 2.7% octylglucoside. with these reconstituted vesicles, a steady state internal na+ concentration was established by oxaloa ... | 1981 | 7028747 |
| the ability of strains of klebsiella aerogenes to survive on the hands. | the ability of klebsiella aerogenes strains isolated from outbreaks of infection, from sporadic infections and from the environment to survive on hands were compared. considered as groups, the outbreak strains survived best and the environmental strains least well. the possible importance of these observations in relation to outbreaks of klebsiella infection in hospitals and to heterogeneity of pathogenic potential and transmissibility in klebsiella aerogenes is discussed. | 1981 | 7031252 |
| microcalorimetric studies of klebsiella aerogenes grown in chemostat culture. 2 c-limited and c-sufficient cultures. | the enthalpy changes for the production of 1 g of cells during a doubling period, delta hg, for cells of k. aerogenes growing aerobically in carbon-limited or carbon-sufficient media in continuous culture have been measured. delta hg varies with the dilution rate, but at a fixed dilution rate delta hg is the same for growth in glucose, glycerol, pyruvate or acetate as the sole carbon and energy source; the molar growth yields are markedly different. delta hg values of cells growing in glucose-su ... | 1981 | 7031438 |
| cloning and expression in escherichia coli k-12 of the genes for major outer membrane protein ompa from shigella dysenteriae, enterobacter aerogenes, and serratia marcescens. | the outer membranes of many gram-negative bacteria contain a major heat-modifiable protein which shows serological cross-reactivity with the ompa protein of escherichia coli k-12. using the cloned gene for the e. coli k12 protein as a dna-dna hybridization probe, we were able to identify the corresponding genes from shigella dysenteriae. enterobacter aerogenes, and serratia marcescens. these were cloned in a phage lambda vector, and their expression in e. coli k-12 was studied. all three ompa pr ... | 1982 | 7033204 |
| phenotypic variability of the sensitivity to cycloserine of klebsiella aerogenes nctc 418, growing in chemostat culture. | the susceptibility of klebsiella aerogenes to cycloserine varied according to the growth conditions. in batch culture, cells were less susceptible to the antibiotic when glycine was present in the medium, presumably due to competition between glycine and cycloserine for the uptake system by which glycine, d-alanine and cycloserine are transported into the cell. in the chemostat at average dilution rates, ammonia-limited cultures were more susceptible to the antibiotic than were glucose-limited c ... | 1981 | 7033466 |
| influence of diluents, media, and membrane filters on detection fo injured waterborne coliform bacteria. | pure cultures of escherichia coli, klebsiella pneumoniae, enterobacter aerogenes, and citrobacter freundii were injured ( greater than 90%) in water from a dead-end section of the bozeman, montana, distribution system. the effects of the following laboratory variables on the enumeration efficiency of injured and undamaged control cells were examined: (i) diluent composition, temperature, and time of exposure; (ii) media, using various formulations employed in enumerating gram-negative bacteria; ... | 1982 | 7034648 |
| the inhibition of bacterial cell growth by ketone bodies. | the effect of ketone bodies on the growth, in culture, of escherichia coli was investigated. both growth and glucose utilisation were inhibited in the presence of 20 mmol/l d-3-hydroxybutyrate. lower concentrations of d-3-hydroxybutyrate caused proportionally less inhibition of growth. acetoacetate also inhibited growth but other glycolytic inhibitors and chemical analogues of d-3-hydroxybutyrate either did not inhibit or proved to be too toxic for bacterial growth. citrate enhanced the ketone b ... | 1981 | 7034705 |
| acute anterior uveitis and klebsiella aerogenes: a casual relationship? | this study was undertaken to investigate the hypothesis that episodes of anterior uveitis in patients with the hla b27 antigen are triggered by the presence of klebsiella aerogenes in the gastrointestinal tract. forty patients were examined. while the recovery of klebsiella was greater in b27 positive patients with uveitis, the differences failed to reach statistical significance. the clinical state of patients with klebsiella aerogenes in their faeces did not differ from that in whom klebsiella ... | 1981 | 7034772 |
| an analysis of discoidin i binding sites in dictyostelium discoideum (nc4). | vegetative wild-type (strain nc4) d. discoideum cells and cells at the 10h stage of development (aggregation) were harvested in the presence of 0.5 m-galactose to remove any endogenous discoidin i already bound to the cell surface, and fixed with glutaraldehyde. affinity-purified 125i-labelled discoidin i bound to these fixed cells in a specific manner, greater than or equal to 95% of binding being inhibited by 0.5 m-galactose. binding of 125i-labelled discoidin i was essentially complete in 90 ... | 1981 | 7036990 |
| the role of biotin and sodium in the decarboxylation of oxaloacetate by the membrane-bound oxaloacetate decarboxylase from klebsiella aerogenes. | the biotin-containing oxaloacetate decarboxylase from klebsiella aerogenes catalyzed the na+-dependent decarboxylation of oxaloacetate to pyruvate and bicarbonate (or co2) but not the reversal of this reaction, not even in the presence of an oxaloacetate trapping system. the enzyme catalyzed an avidin-sensitive isotopic exchange between [1-14c]pyruvate and oxaloacetate, which indicated the intermediate formation of a carboxybiotin enzyme. sodium ions were not required for this partial reaction, ... | 1982 | 7037395 |
| the generation of an electrochemical gradient of sodium ions upon decarboxylation of oxaloacetate by the membrane-bound and na+-activated oxaloacetate decarboxylase from klebsiella aerogenes. | | 1982 | 7037396 |
| a sequential study of the relationship between faecal klebsiella aerogenes and the common clinical manifestations of ankylosing spondylitis. | an 8-month sequential study at 4-weekly intervals of faecal klebsiella aerogenes and clinical activity of ankylosing spondylitis is described. similar frequencies of faecal k. aerogenes were found in the 44 patients and 36 healthy controls studied. eighteen patients on 19 occasions had k. aerogenes cultured from their faeces, when the preceding specimen had been negative. six (31.6%) of these occasions were associated with a deterioration in clinical state compared with a similar deterioration a ... | 1982 | 7039521 |
| primary structure of the chromosomal origins (oric) of enterobacter aerogenes and klebsiella pneumoniae: comparisons and evolutionary relationships. | the nucleotide sequences of the enterobacter aerogenes and klebsiella pneumoniae dna replication origins (oric) were determined and compared with those of escherichia coli and salmonella typhimurium. four interrelated, 9-base-pair repeats were identified from the conserved regions within the minimal origin. evolutionary rates calculated from the minimal origin sequences yielded a quantitative phylogenic tree which agreed with the taxonomic classification of these genera. | 1982 | 7042695 |
| an epizootic of klebsiella aerogenes infection in laboratory rats. | klebsiella aerogenes was identified as the pathogen in an epizootic of single and multiple abscesses in the cervical, axillary and inguinal regions in laboratory rats. involvement of the salivary glands in the inflammatory tissue in some cases produced interlobular and interacinar fibrosis resembling "fibroadenosis'. similar lesions were reproduced with a strain of klebsiella aerogenes isolated from a diseased rat. oral carriage of the same strain was detected in the diseased rats, and in normal ... | 1981 | 7043077 |
| microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salts/glucose media. 3. the effect of growth temperature on energy conversion. | calorimetric and growth data are reported for the growth of k. aerogenes in glucose-limited media over the temperature range 25 degrees to 42 degrees c. rate constants for heat output, co2 evolution and biomass production are maximal at 37 degrees c; the rate constant for heat output is greater than that for biomass production at growth temperatures less than 37 degrees c. changes in the power-time profile indicate that different metabolic processes occur at the different temperatures even when ... | 1981 | 7043213 |
| expression in escherichia coli k12 of the cloned genes for a major outer membrane protein (ompa protein) from shigella dysenteriae, enterobacter aerogenes and serratia marcescens. | | 1982 | 7044211 |
| the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract. | the development of the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract was studied in 31 infants during the first 10 days of life. specimens were collected from the umbilicus, mouth and gastro-intestinal tract on the pre-operative day, at operation and on post-operative days 1, 2, 3, 5, 7 and 10. bacteria were isolated semi-quantitatively on a variety of plain and selective media and identified by conventional methods.staphylococcus albus was the predomin ... | 1982 | 7057028 |
| inhibition of chorismate mutase activity of chorismate mutase-prephenate dehydrogenase from aerobacter aerogenes. | inhibition data (i50 values) have been obtained for inhibitors of the chorismate mutase activity of chorismate mutase-prephenate dehydrogenase from aerobacter aerogenes. several 1-substituted adamantane derivatives were investigated; the order of decreasing inhibitory activity with the various substituents was -po32- much greater than -p(och3)o2- greater than -co2- greater than -ch2co2- greater than -so2- greater than so3-. 3-chloroadamantane-1-acetic acid was slightly less effective than adaman ... | 1982 | 7093222 |
| unilateral palpable purpura. a manifestation of septic emboli from an infected aortofemoral bypass graft eroding the jejunum. | a 48-year-old man had a palpable purpuric eruption of the right lower extremity several years after a right aortofemoral bypass graft for obstructive atherosclerotic disease. histologic examination revealed a leukocytoclastic angitis, and belated tissue gram's stains disclosed clumps of gram-negative organisms. the focus for the unilateral purpura proved to be an erosion of the jejunum by the bypass that yielded enterobacter aerogenes on culture, as did one preoperative blood sample. | 1981 | 7259223 |
| in vitro synergism of fr-31564, a new phosphonic acid antibiotic. | against most test strains of gram-negative bacilli, the in vitro effect of fr-31564 together with beta-lactam antibiotics or trimethoprim was strongly synergistic; with tetracycline and nalidixic acid the effect was additive; and with gentamicin and sulfamethoxazole the effect was additive or antagonistic. fr-31564 was markedly synergistic with beta-lactam antibiotics against beta-lactam antibiotic-resistant gram-negative bacilli such as klebsiella pneumoniae, enterobacter aerogenes, e. cloacae, ... | 1981 | 7287590 |
| structure of the capsular polysaccharide of klebsiella type 73 (enterobacter aerogenes). | the capsular polysaccharide from klebsiella type 73 (enterobacter aerogenes) was found to contain equimolar amounts of d-galactose, d-glucose, l-rhamnose, and d-glucuronic acid. acid hydrolysis of the polysaccharide gave one aldobio-and one aldotrio-uronic acid, whose structures were established by acid hydrolysis and by methylation analysis. the anomeric configurations of the different sugar residues were determined from the specific rotations of the polysaccharide and the aldobio-and aldotrio- ... | 1981 | 7317881 |
| [sensibility of rabbits to treatment with ampicillin and gentamycin (author's transl)]. | ampicillin administered to rabbits (20 mg/kg per day) over a period of three days subsequently provoked a death rate of 40%. no deaths were observed after treatment with gentamycin (10 mg/kg per day) or with a combination of ampicillin and gentamycin. on the fourth day weight loss in the three treated groups was 12%, 11% and 9%, respectively, compared to controls. at the same time food intake in the treated groups was only 15% to 20% of the amount consumed by controls. in the caecum, ampicillin ... | 1981 | 7342822 |
| fermentation of polysaccharides by klebsielleae and other facultative bacilli. | fermentations of 10 polysaccharides by species of the family enterobacteriaceae were examined. algin, guar, karaya, xanthan, and xylan were not fermented by any of the strains tested. most of the activity was found in the tribe klebsielleae. klebsiella oxytoca fermented amylopectin (97% of the strains studied), carrageenan (100%), inulin (68%), polypectate (100%), and tragacanth (100%). klebsiella pneumoniae fermented amylopectin (91%), carrageenan (100%), and tragacanth (86%). carrageenan was a ... | 1980 | 7396489 |
| bactericidal killing activities of cefepime, ceftazidime, cefotaxime, and ceftriaxone against staphylococcus aureus and beta-lactamase-producing strains of enterobacter aerogenes and klebsiella pneumoniae in an in vitro infection model. | cefepime (cp) is a new injectable cephalosporin with a broad spectrum of activity and stability against common chromosomally and plasmid-mediated beta-lactamases. the bactericidal activities of cp, ceftazidime (cz), cefotaxime (ctx), and ceftriaxone (cax) against reference and clinical strains of staphylococcus aureus, an isogenic pair of enterobacter aerogenes strains (wild type and a cz-resistant derepressed mutant), and a klebsiella pneumoniae isolate possessing a tem-10 beta-lactamase were i ... | 1995 | 7486916 |
| bacterial agents causing chronic suppurative otitis media. | ear swabs from 350 patients with chronic otitis media attending different orthorhinolaryngological clinics at different hospitals and health centres in benin city and ekpoma in edo state were screened for the presence of bacterial agents of chronic otitis media. results revealed the presence of 19 different species indicating polymicrobial infections. species isolated comprised staphylococcus aureus (33.6%), pseudomonas aeruginosa (19.3%), proteus mirabilis (17%), alcaligenes faecalis (6.2%) and ... | 1995 | 7498006 |
| cloning and characterization of a tryptophanase gene from enterobacter aerogenes sm-18. | a tryptophanase gene from enterobacter aerogenes sm-18 was cloned and sequenced. the structural gene for tryptophanase, tnaa, consisted of 1389 bp encoding 462 amino acid residues, and its nucleotide sequence and deduced amino acid sequence showed significant homology to those of tnaa from escherichia coli k12. a short open reading frame consisting of 31 amino acid residues was found upstream of tnaa, and it showed some similarity to the e. coli tnac gene known to be a cis-acting regulatory elem ... | 1993 | 7510326 |
| prolonged stability of r plasmids kr61 and kr61-a in salmonella host. | the r plasmids, kr61 and kr61-a, that were originally isolated from a clinical strain of aerobacter aerogenes in 1971 and determined resistance to kanamycin (km), neomycin (nm), streptomycin (sm), tetracycline (tc); and ampicillin (ap) respectively were found stable in salmonella typhimurium lt2 even after 22 years of cultivation on antibiotic free media. kr61, carrying resistance to kmnmsmtc, not only maintained all its resistances but also maintained its conjugal transferability (rtf) as indic ... | 1995 | 7558189 |
| activation of transcription initiation from the nac promoter of klebsiella aerogenes. | the nac gene of klebsiella aerogenes encodes a bifunctional transcription factor that activates or represses the expression of several operons under conditions of nitrogen limitation. in experiments with purified components, transcription from the nac promoter was initiated by sigma 54 rna polymerase and was activated by the phosphorylated form of nitrogen regulator i (nri) (ntrc). the activation of the nac promoter required a higher concentration of nri approximately p than did the activation o ... | 1995 | 7559338 |
| repression of the klebsiella aerogenes nac promoter. | transcription of the nitrogen-regulated nac promoter of klebsiella aerogenes requires sigma54 rna polymerase, is activated by the phosphorylated form of the transcription factor nitrogen regulator i (nri) (ntrc), and is repressed by the product of the nac gene, nac. nac protects a large portion of the nac control region, extending from positions -130 to -70, from digestion by dnase i. this site(s) lies immediately upstream from the site at which sigma 54 rna polymerase binds, is downstream of a ... | 1995 | 7559339 |
| molecular biology of microbial ureases. | urease (urea amidohydrolase; ec 3.5.1.5) catalyzes the hydrolysis of urea to yield ammonia and carbamate. the latter compound spontaneously decomposes to yield another molecule of ammonia and carbonic acid. the urease phenotype is widely distributed across the bacterial kingdom, and the gene clusters encoding this enzyme have been cloned from numerous bacterial species. the complete nucleotide sequence, ranging from 5.15 to 6.45 kb, has been determined for five species including bacillus sp. str ... | 1995 | 7565414 |
| ceftibuten: a review of antimicrobial activity, spectrum and other microbiologic features. | ceftibuten is a new, orally administered cephalosporin with exceptional beta-lactamase stability and potency against commonly isolated gram-negative pathogens. more than 90% of recent enterobacteriaceae clinical isolates were inhibited by < or = 8 micrograms/ml of ceftibuten. in only five enteric species (citrobacter freundii, enterobacter aerogenes, enterobacter cloacae, morganella morganii, serratia marcescens) were more than 15% of strains resistant (minimal inhibitory concentrations (mic, wi ... | 1995 | 7567314 |
| membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry. | the ionophore, valinomycin, was investigated as a possible means of bacterial viability assessment using the fluorescent membrane potential dye rhodamine 123. membrane hyperpolarisation in escherichia coli, pseudomonas fluorescens, enterobacter aerogenes and arthrobacter globiformis was examined during exponential growth and during stress by brief starvation in a high sodium, low potassium buffer using flow cytometric analysis of rhodamine 123 uptake. dye uptake was variable both between species ... | 1995 | 7590182 |
| a klebsiella aerogenes moaef operon is controlled by the positive moar regulator of the monoamine regulon. | a 30-kda protein accumulated upon induction by a high concentration of tyramine or dopamine in cells of klebsiella aerogenes (ka). these cells carried a plasmid (pas123) that included the arylsulfatase operon (atsba). deletion analysis showed that the region essential for induction of the 30-kda protein was located within a 2.0-kb cloned segment downstream of the atsba operon. the nucleotide (nt) sequence of the 2.0-kb fragment revealed two open reading frames (orfs), moae and moaf. transcriptio ... | 1995 | 7590328 |
| molecular cloning, mapping, and regulation of pho regulon genes for phosphonate breakdown by the phosphonatase pathway of salmonella typhimurium lt2. | two pathways exist for cleavage of the carbon-phosphorus (c-p) bond of phosphonates, the c-p lyase and the phosphonatase pathways. it was previously demonstrated that escherichia coli carries genes (named phn) only for the c-p lyase pathway and that enterobacter aerogenes carries genes for both pathways (k.-s. lee, w. w. metcalf, and b. l. wanner, j. bacteriol. 174:2501-2510, 1992). in contrast, here it is shown that salmonella typhimurium lt2 carries genes only for the phosphonatase pathway. ge ... | 1995 | 7592415 |
| multicentre comparative study on the antibacterial activity of fk-037, a new parenteral cephalosporin. | the in vitro antibacterial activity of fk-037, a new parenteral cephalosporin structurally related to cefpirome and cefepime, was compared with that of cefotaxime, ceftazidime, aztreonam, cefpirome, cefepime, imipenem and meropenem against 1,837 clinical isolates obtained from three spanish hospitals. fk-037 inhibited 90% of enterobacteriaceae isolates at < or = 0.25 microgram/ml, with the exception of enterobacter aerogenes (mic90 1 microgram/ml), enterobacter cloacae and citrobacter freundii ( ... | 1995 | 7614970 |
| decrease in culturability of vibrio cholerae caused by glucose. | the culturability of vibrio cholerae o1 serotype inaba strain 569b was decreased by the addition of glucose to cell suspensions in starvation media. a similar effect was observed with sucrose, maltose, and fructose. we term this inhibitory effect glucose shock. it was not observed with arabinose or xylose or with carboxylates, such as acetate and pyruvate. no acidification of the medium occurred in the presence of these carbohydrates. glucose shock was prevented by the addition of nitrogen or ph ... | 1995 | 7618870 |
| characterization of nosocomial strains of enterobacter aerogenes by arbitrarily primed-pcr analysis and ribotyping. | to study the spread of strains of enterobacter aerogenes in our hospital in 1992 and 1993 by using two genotypic markers, and to evaluate these methods for the epidemiological investigation of this species. | 1995 | 7636170 |
| control of infection with multiple antibiotic resistant bacteria in a hospital renal unit: the value of plasmid characterization. | an outbreak of infections due to multiple antibiotic-resistant bacteria took place over a period of approximately 18 months in a renal unit. strains of escherichia coli, enterobacter aerogenes, klebsiella pneumoniae, citrobacter spp. and pseudomonas spp. were involved, and a variety of antibiotic resistances was encountered. closely related plasmids encoding resistance to aztreonam, ceftazidime and piperacillin, possibly derived from an archetypal plasmid of 105 kb were found in the majority of ... | 1995 | 7641839 |
| activation of the escherichia coli lacz promoter by the klebsiella aerogenes nitrogen assimilation control protein (nac), a lysr family transcription factor. | a chimeric promoter with the nitrogen assimilation control protein binding site from hutup of klebsiella aerogenes fused to the lacz core promoter from escherichia coli was built and cloned in a lacz reporter plasmid. this construct showed a 14-fold increase of beta-galactosidase activity upon nitrogen limitation. primer extension experiments showed that the nitrogen assimilation control protein activates laczp1 in a position-dependent manner. | 1995 | 7642513 |
| identification of a global repressor gene, rsma, of erwinia carotovora subsp. carotovora that controls extracellular enzymes, n-(3-oxohexanoyl)-l-homoserine lactone, and pathogenicity in soft-rotting erwinia spp. | the production of extracellular enzymes such as pectate lyase (pel), polygalacturonase (peh), cellulase (cel), and protease (prt) is activated by the cell density (quorum)-sensing signal, n-(3-oxohexanoyl)-l-homoserine lactone (hsl); plant signals; and aep genes during postexponential growth of erwinia carotovora subsp. carotovora 71. studies with mutants of e. carotovora subsp. carotovora 71 derepressed in exoenzyme production led to the identification of a negative regulator gene, rsma (rsm, r ... | 1995 | 7665490 |
| surveillance of gram-negative bacteria for pseudomonas aeruginosa lipoprotein i using a monoclonal antibody. | we prepared an igg1 murine monoclonal antibody, ps2, that recognized the 8-kda outer membrane lipoprotein i of pseudomonas aeruginosa. the biodot-immunoblot technique was used to test 470 bacterial isolates for their reactivity with mab ps2. ps2 recognized 298 of 300 p. aeruginosa clinical isolates for a sensitivity of 99.3%. ps2 reacted with all fisher-devlin immunotype and international antigenic type p. aeruginosa strains. forty-seven isolates of other pseudomonas species were tested and only ... | 1993 | 7689535 |
| demonstration of the indolepyruvate decarboxylase gene homologue in different auxin-producing species of the enterobacteriaceae. | different enterobacteriaceae were assayed for their ability to produce the plant hormone indole-3-acetate with the aim to study the distribution of the indole-3-pyruvate pathway, which is known to be involved in the production of indole-3-acetate in a root-associated enterobacter cloacae strain. other e. cloacae strains, and also enterobacter agglomerans strains, pantoea agglomerans, klebsiella aerogenes, and klebsiella oxytoca were found to convert tryptophan into indole-3-acetate. as it was al ... | 1994 | 7704833 |
| random mutagenesis of pullulanase from klebsiella aerogenes for studies of the structure and function of the enzyme. | to study the structure and function of pullulanase from klebsiella aerogenes, a method involving random mutagenesis of the entire gene for pullulanase was used. out of 50,000 clones screened at high temperature, seven genes for mutant proteins were identified by dna sequencing. the amino acid substitutions in the seven mutant proteins were clustered on the nh2-terminal side of the four conserved regions found in alpha-amylases. these mutant pullulanases were classified into two types: those whos ... | 1994 | 7706211 |
| energy-dispersive x-ray analysis of the extracellular cadmium sulfide crystallites of klebsiella aerogenes. | klebsiella aerogenes forms electron-dense particles on the cell surface in response to the presence of cadmium ions in the growth medium. these particles ranged from 20 to 200 nm in size, and quantitative energy dispersive x-ray analysis established that they comprise cadmium and sulfur in a 1:1 ratio. this observation leads to the conclusion that the particles are cadmium sulfide crystallites. a combination of atomic absorption spectroscopy, inductively coupled plasma mass spectrometry, and aci ... | 1995 | 7710328 |
| evidence for the presence of urease apoprotein complexes containing ured, uref, and ureg in cells that are competent for in vivo enzyme activation. | in vivo activation of klebsiella aerogenes urease, a nickel-containing enzyme, requires the presence of functional ured, uref, and ureg accessory proteins and is further facilitated by uree. these accessory proteins are proposed to be involved in metallocenter assembly (m. h. lee, s. b. mulrooney, m. j. renner, y. markowicz, and r. p. hausinger, j. bacteriol. 174:4324-4330, 1992). a series of three ured-urease apoprotein complexes are present in cells that express ured at high levels, and these ... | 1995 | 7721685 |
| molecular epidemiology of infections due to enterobacter aerogenes: identification of hospital outbreak-associated strains by molecular techniques. | molecular techniques were used to study the epidemiology of infections due to enterobacter aerogenes in a tertiary-care hospital. sixty-two clinical isolates were collected from 43 patients over 3 months. restriction endonuclease analysis (rea) of chromosomal dna and repetitive-element polymerase chain reaction (rep-pcr) with primers based on repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (eric) bacterial dna sequences were used for genomic fingerprin ... | 1995 | 7727676 |
| cloning and nucleotide sequence of the signal peptidase ii (lsp)-gene from staphylococcus carnosus. | staphylococcus carnosus tm300 is able to synthesize at least seven lipoproteins with molecular masses between 15 and 45 kda; the proteins are located in the membrane fraction. it can be concluded that this strain also posesses the enzymes involved in lipoprotein modification and prolipoprotein signal peptidase (signal peptidase ii) processing. the gene encoding the prolipoprotein signal peptidase, lsp, from staphylococcus carnosus tm300 was cloned in escherichia coli and sequenced. the deduced a ... | 1995 | 7729667 |
| a histidine decarboxylase gene encoded by the vibrio anguillarum plasmid pjm1 is essential for virulence: histamine is a precursor in the biosynthesis of anguibactin. | we have identified and sequenced an hdc gene in the vibrio anguillarum plasmid pjm1 which encodes a histidine decarboxylase enzyme and is an essential component for the biosynthesis of anguibactin. the open reading frame corresponds to a protein of 386 amino acids with a calculated molecular mass of 44,259.69 da. the amino acid sequence has extensive homology with the pyridoxal-p-dependent histidine decarboxylases of morganella morganii, klebsiella planticola, and enterobacter aerogenes. tn3-hoh ... | 1995 | 7752899 |
| the crystal structure of urease from klebsiella aerogenes. | the crystal structure of urease from klebsiella aerogenes has been determined at 2.2 a resolution and refined to an r factor of 18.2 percent. the enzyme contains four structural domains: three with novel folds playing structural roles, and an (alpha beta)8 barrel domain, which contains the bi-nickel center. the two active site nickels are 3.5 a apart. one nickel ion is coordinated by three ligands (with low occupancy of a fourth ligand) and the second is coordinated by five ligands. a carbamylat ... | 1995 | 7754395 |
| direct sequencing of superoxide dismutase genes from two bacterial strains amplified by polymerase chain reaction. | the nucleotides of the mn-superoxide dismutase (sod) gene of bacillus circulans and the fe-sod gene of aerobacter aerogenes were sequenced by pcr. these sod genes were specifically amplified by using oligonucleotide primers corresponding to the amino-terminal amino acid sequences and the antisense strand primer corresponding to the common amino acid sequence near the carboxyl-terminus among various mn- and fe-sods thus far sequenced. the pcr products amplified from b. circulans and a. aerogenes ... | 1993 | 7764218 |
| purification, characterization, and crystallization of monoamine oxidase from escherichia coli k-12. | the gene for monoamine oxidase (mao) was cloned from an escherichia coli genomic library and mao was overproduced in the periplasmic space. the enzyme was purified to homogeneity by preparation of a periplasmic fraction, followed by ammonium sulfate fractionation and deae-cellulose column chromatography. crystals were obtained by the hanging drop method using sodium citrate as a precipitant. the enzyme was found to be a dimer of identical subunits with a molecular weight of 80,000, and showed th ... | 1994 | 7765483 |
| the nitrogen assimilation control protein, nac, is a dna binding transcription activator in klebsiella aerogenes. | a 32-kda polypeptide corresponding to nac, the product of the klebsiella aerogenes nac gene, was overexpressed from a plasmid carrying a tac'-'nac operon fusion and purified to near homogeneity by taking advantage of its unusual solubility properties. nac was able to shift the electrophoretic migration of dna fragments carrying the nac-sensitive promoters hutup, putpp1, and uredp. the interaction between nac and hutup was localized to a 26-bp region centered approximately 64 bp upstream of the h ... | 1995 | 7768865 |
| [resistance to imipenem in enterobacter aerogenes]. | in june, 1993, an enterobacter aerogenes strain was isolated in the hospital de la creu roja from hospitalet de llobregat (barcelona), which was resistant against all beta-lactams, including imipenem. since is unusual in enterobacter sp. to isolate imipenem resistant strains, we decided to study its resistance mechanism. | 1995 | 7779892 |
| significance of antibiotics resistance amongst clinical bacterial isolates in lagos. | in vitro susceptibility of several strains of six different species of clinical facultative pathogens involved in nosocomial infections in our hospital was investigated by a series of disc diffusion, broth dilution and chequerboard titration testing. with disc diffusion method all the test strains, except streptococcus pyogenes, were resistant to penicillin. 46% of the klebsiella aerogenes and 73% of the pseudomonas strains were generally resistant to cefotaxime. the minimum inhibitory concentra ... | 1994 | 7803333 |
| the urease structural gene urea in rhizobium meliloti is preceded by an open reading frame necessary for urease activity. | an open reading frame (orf1) located upstream of the urease structural gene urea in rhizobium meliloti strain ak631 was cloned and characterized by dna sequencing. comparison of the amino acid sequence revealed partial homology with the urease accessory gene ured of klebsiella aerogenes and proteus mirabilis. mutational analysis of orf1 showed that the gene is necessary for urease activity. its function is still unknown. | 1994 | 7813887 |
| detection of resistance due to inducible beta-lactamase in enterobacter aerogenes and enterobacter cloacae. | thirty-six of 36 strains of enterobacter cloacae and e. aerogenes with inducible beta-lactamase developed resistance when cefoxitin (inducer) was added to cefuroxime disks. constitutive beta-lactamase producers (n = 23) were all resistant to cefuroxime. cefuroxime resistance correlated with the amount of induced or constitutive beta-lactamase. cefuroxime was a better indicator of induced resistance than cefamandole, cefazolin, cephalothin, ceftriaxone, cefotaxime, ticarcillin with or without cla ... | 1994 | 7814486 |
| development of a continuous coupled enzymatic assay for myo-inositol monophosphatase. | myo-inositol monophosphatase, an enzyme purified from brain tissues, catalyses the dephosphorylation of myo-inositol 1-phosphate. this enzyme has become the subject of intense research interest since myo-inositol is needed for the resynthesis of phosphatidylinositol in cell membranes. since phosphate contamination has always been a problem for the assay of this enzyme activity, we have developed a coupled enzymatic assay for detecting the activity of the phosphatase with no interference by the p ... | 1994 | 7836662 |
| in vitro activity of cp-99,219, a novel 7-(3-azabicyclo[3.1.0]hexyl) naphthyridone antimicrobial. | the in vitro activity of cp-99,219 was compared with that of ciprofloxacin and sparfloxacin against 814 clinical bacterial isolates using a microdilution method with brain-heart infusion broth. cp-99,219 was the most potent agent tested against methicillin-resistant, ciprofloxacin-susceptible staphylocci (minimum inhibitory concentration [mic]90 < or = 0.25 microgram/ml). cp-99,219 was 32-fold and fourfold more potent than ciprofloxacin and sparfloxacin, respectively, against streptococcus pneum ... | 1994 | 7851087 |
| requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter. | assembly of protein metallocenters is not well understood. urease offers a tractable system for examination of this process. formation of the urease metallocenter in vivo is known to require four accessory proteins: ured, postulated to be a urease-specific molecular chaperone; uree, a nickel(ii)-binding protein; and uref and ureg, of unknown function. activation of purified klebsiella aerogenes urease apoprotein was accomplished in vitro by providing carbon dioxide (half-maximal activation at ap ... | 1995 | 7855593 |
| degradation of propanil by bacterial isolates and mixed populations from a pristine lake. | the microbial transformation rates of propanil, a commonly used herbicide, were investigated using water from a pristine lake in northeast georgia. microbial degradation rates were measured using natural water microflora, the natural water microflora amended with five bacterial species (aerobacter aerogenes, aeromonas hydrophila, acinetobacter calcoaceticus, proteus mirabilis, and aeromonas salmonicida) isolated from the same lake, and the five isolates individually. transformation rate constant ... | 1995 | 7874462 |
| the amino acid sequence of lrp is highly conserved in four enteric microorganisms. | lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in escherichia coli (j. m. calvo and r. g. matthews, microbiol. rev. 58:466-490, 1994). the lrp genes from three other enteric microorganisms, enterobacter aerogenes, klebsiella aerogenes, and salmonella typhimurium, were cloned and sequenced. an analysis of these sequences and of the previously determined sequence from e. coli indicated that the vast majority of changes were synonymous rather than nonsynonymous chan ... | 1995 | 7883720 |
| the alcaligenes eutrophus protein hoxn mediates nickel transport in escherichia coli. | hoxn, an integral membrane protein with seven transmembrane helices and a molecular mass of 33.1 kda, is involved in high-affinity nickel transport in alcaligenes eutrophus h16. from genetic analyses, it has been concluded that hoxn is a single-component ion carrier. to investigate this assumption, hoxn was introduced into escherichia coli. the recombinant strain showed significantly enhanced nickel uptake in a short-interval assay. likewise, growth in the presence of 63nicl2 yielded a more than ... | 1995 | 7896709 |
| the occurrence of duplicate lysyl-trna synthetase gene homologs in escherichia coli and other procaryotes. | the lysyl-trna synthetase (lysrs) system of escherichia coli k-12 consists of two genes, lyss, which is constitutive, and lysu, which is inducible. it is of importance to know how extensively the two-gene lysrs system is distributed in procaryotes, in particular, among members of the family enterobacteriaceae. to this end, the enterics e. coli k-12 and b; e. coli reference collection (ecor) isolates ec2, ec49, ec65, and ec68; shigella flexneri; salmonella typhimurium; klebsiella pneumoniae; ente ... | 1995 | 7896714 |
| in vitro activation of urease apoprotein and role of ured as a chaperone required for nickel metallocenter assembly. | the formation of active urease in klebsiella aerogenes requires the presence of three structural genes for the apoprotein (urea, ureb, and urec), as well as four accessory genes (ured, uree, uref, and ureg) that are involved in functional assembly of the metallocenter in this nickel-containing enzyme. slow and partial activation of urease apoprotein was observed after addition of nickel ion to extracts of escherichia coli cells bearing a plasmid containing the k. aerogenes urease gene cluster or ... | 1994 | 7909161 |
| cloning, sequence and characterization of the polyphosphate kinase-encoding gene (ppk) of klebsiella aerogenes. | polyphosphate kinase (ppk) catalyzes the formation of polyphosphate (polyp). the ppk-encoding gene (ppk) has been cloned from klebsiella aerogenes atcc9621. the gene possessed an open reading frame of 2055 bp capable of encoding a putative polypeptide with a deduced m(r) of 80,157. this polypeptide showed 93% similarity to the escherichia coli ppk. the nucleotide sequence of the promoter region of k. aerogenes ppk differed from that of the previously sequenced e. coli ppk. a putative pho box seq ... | 1993 | 7916727 |
| chromate reductase activity of enterobacter aerogenes is induced by nitrite. | a chromate resistant mutant of enterobacter aerogenes manifested its chromate resistance only under aerobic conditions. both parent and mutant showed substantial levels of anaerobic chromate reductase activity when grown on glycerol plus fumarate. the chromate reductase was further induced by growth in the presence of nitrite but was repressed by nitrate. the chromate reductase activity paralleled that of the formate-linked nitrite reductase. there was no significant difference in chromate reduc ... | 1994 | 7988866 |
| consecutive mutations leading to the emergence in vivo of imipenem resistance in a clinical strain of enterobacter aerogenes. | three consecutive isolates of enterobacter aerogenes were obtained from the blood cultures of a hospitalised patient who was receiving antibiotic therapy. the initial isolate possessed an inducible cephalosporinase and was susceptible to third-generation cephalosporins. after ceftazidime treatment, a second isolate resistant to this antibiotic and characterised by stable overproduction of the chromosomal beta-lactamase was obtained, and therapy was altered to a new combination which included imi ... | 1994 | 8006932 |
| cloning and characterization of a tryptophanase gene from enterobacter aerogenes sm-18. | | 1994 | 8012613 |
| production of polyhydroxyalkanoates in sucrose-utilizing recombinant escherichia coli and klebsiella strains. | the cloned poly-3-hydroxybutyrate (phb) synthesis pathway from alcaligenes eutrophus has been introduced into sucrose-utilizing strains of escherichia coli, klebsiella aerogenes, and klebsiella oxytoca. the plasmid-borne genes were well expressed in these environments and were able to mediate the production of significant amounts of phb when the bacteria were grown with sucrose as the sole carbon source. the molecular weight of the phb polymer made in k. aerogenes and e. coli was approximately 1 ... | 1994 | 8017916 |
| evaluation of rapid one system for identification of 379 strains in the family enterobacteriaceae and oxidase-negative, gram-negative nonfermenters. | the ability of the rapid one system (innovative diagnostic systems, inc., norcross, ga.) to identify 364 strains in the family enterobacteriaceae and 15 oxidase-negative, gram-negative, nonfermentative rods was evaluated. kits were inoculated with no. 2 mcfarland standard suspensions, and reactions were interpreted after 4 h of incubation at 35 degrees c. overall, the method correctly identified (to the species level or to the genus level for salmonellas and non-shigella sonnei shigella species) ... | 1994 | 8027345 |
| chiral inversion of drug: role of intestinal bacteria in the stereoselective sulphoxide reduction of flosequinan. | chiral inversion at a sulphoxide position of flosequinan enantiomers [(+/-)-7-fluoro-1-methyl-3-methylsulphinyl-4-quinolone] occurred in conventional rats but not in either germ-free rats or rats treated with antibiotics after an oral administration of each enantiomer. thus, it was postulated that the chiral inversion occurred by mechanisms mediated by intestinal bacteria. the intestinal content isolated from conventional rats reduced r(+)- and s(-)-flosequinan to produce the sulphide, while int ... | 1994 | 8053920 |
| antimicrobial activity of essential oil from schinus molle linn. | the essential oil from the fresh leaves of schinus molle isolated by hydrodistillation was tested for antibacterial activity using the hole plate diffusion method and for antifungal activity using the mycelium or single cell growth inhibition method. results obtained showed that the volatile oil exhibited significant activity against the following bacterial species: klebsiella pneumoniae, alcaligenes faecalis, pseudomonas aeruginosa, leuconostoc cremoris, enterobacter aerogenes, proteus vulgaris ... | 1993 | 8055554 |
| on the evolution of arginases and related enzymes. | sequence analysis of the arginase/agmatine ureohydrolase family, important enzymes in arginine/agmatine metabolism and the urea cycle, reveals the similarity of arginases to formiminoglutamate hydrolase (hutg) in klebsiella aerogenes and to a previously unidentified open reading frame adjacent to the hmf locus of the archaebacterium methanothermus fervidus. the gene structure and distribution of these homologous proteins across primary kingdoms suggest that this family is another example of a pr ... | 1994 | 8064866 |
| roles of catabolite activator protein sites centered at -81.5 and -41.5 in the activation of the klebsiella aerogenes histidine utilization operon hutuh. | the klebsiella aerogenes hutuh operon is preceded by a promoter region, hut(p), that contains two divergent promoters (hutup and pc) which overlap and are alternately expressed. in the absence of the catabolite gene activator protein-cyclic amp (cap-camp) complex, pc is predominantly expressed while hutup is largely repressed. cap-camp has the dual effect of repressing transcription from pc while simultaneously activating transcription from hutup. dna deletion mutations in this region were used ... | 1994 | 8071230 |
| identification of the hutuh operator (hutuo) from klebsiella aerogenes by dna deletion analysis. | expression of klebsiella aerogenes histidine utilization operons hutuh and hutig is negatively regulated by the product of hutc. multiple copies of the hutuh promoter region [hut(p)] present in trans were able to titrate the limited amount of host-encoded hut repressor (hutc). thus, the hut(p) region contains a specific binding site for hutc. to identify dna sequences required for hutc titration, we constructed and characterized a set of 40 left-entering and 28 right-entering deletions within a ... | 1994 | 8071231 |
| [comparative study of single strand conformation polymorphism of 4.5s rna gene in enterobacteria]. | a recently developed technique, non-isotopic single strand conformation polymorphism analysis (pcr-sscp), was applied to study the conserved feature of 4.5s rna gene in enterobacteria. the 4.5s rna gene was amplified by the polymerase chain reaction, using the template dna extracted respectively from five strains of escherichia coli and three strains of different genera in enterobacteriaceae, i.e. proteus vulgaris, serratia marcescens and enterobacter aerogenes. the pcr products were then carrie ... | 1994 | 8073757 |