| human monoclonal antibodies to influenza virus: igg subclass and light chain distribution. | three adults and three children were immunized with inactivated or live attenuated influenza vaccines and 98 igg monoclonal antibodies derived from ebv immortalization of their blood lymphocytes were studied. all antibodies reacted specifically with influenza a h3n2 or h1n1 whole virus and 73 of 74 tested reacted with the purified ha glycoproteins. the majority (76%) of 77 monoclonal antibodies adequately tested by elisa or solid phase ria contained lambda light chains. elisa analysis of the igg ... | 1989 | 2545224 |
| epstein-barr virus: the hematologic and oncologic consequences of virus-host interaction. | varicella-zoster virus (vzv) and epstein-barr virus (ebv) are two of the human herpesviruses. the others include herpes simplex virus (hsv) type 1, hsv type 2, and cytomegalovirus (cmv). in a series of two articles, we review the clinical diseases caused by vzv and ebv infections; we pay particular attention to the manifestations of these two viral infections in immunosuppressed and immunocompromised patients. in addition to the clinical reviews, each of the two articles begins with a brief disc ... | 1989 | 2545365 |
| [a case of meningoencephalitis caused by persistent epstein-barr (e-b) virus infection]. | a 44-year-old man had several episodes of tingling sensation in his lower extremities, head and face over 1 year and presented mental disturbance 2 months prior to admission. he additionally suffered from dysuria and acute onset of gait disturbance 18 days before admission. physical and neurological examination revealed marked splenomegaly, stupor, abnormal behavior, spastic tetraparesis and sphincter disturbances, and meningoencephalitis was suspected. there was lymphocytosis in peripheral bloo ... | 1989 | 2545401 |
| herpesvirus infections in the immunocompromised host. clinical spectrum, diagnosis, management and prophylaxis. | | 1989 | 2545413 |
| identification of epstein-barr virus-infected cells in tonsils of acute infectious mononucleosis by in situ hybridization. | in situ hybridization with 35s-labeled epstein-barr virus (ebv) probes was applied to paraffin sections of tonsils from seven patients with clinical, serologic, and morphologic evidence of acute infectious mononucleosis. ebv genomes were demonstrated in activated lymphoid b blasts in the interfollicular and perifollicular zones in all these cases. however, in no case could ebv be identified in epithelial cells. these results are at variance with the current concept which attributes a central rol ... | 1989 | 2545594 |
| the transforming prototype of epstein-barr virus (b95-8) is also a lytic virus. | the b95-8 isolate of the epstein-barr virus (ebv) has been described as a non-lytic transforming virus. we have performed experiments in order to determine if the b95-8 ebv is capable of super-infecting and replicating in ebv-genome-positive non-producer lymphoblastoid cells. using concentrates of b95-8 ebv, prepared from 6 different b95-8 cell lines treated with 12-o-tetradecanoylphorbol-13-acetate (tpa), we demonstrated that virus concentrates could transform human or cotton-top tamarin b-lymp ... | 1989 | 2545637 |
| oral manifestations of hiv infection in 75 dutch patients. | seventy-five consecutive hiv-infected patients, including 33 aids cases were examined. one or more oral mucosal lesions were observed in 57 (76%); candidiasis was the most common finding (52%). others included hairy leukoplakia (16%), periodontal disease (16%) and kaposi's sarcoma (4%). diversity of study design and methods allowed no reliable comparison with other reports. | 1989 | 2545871 |
| induction of anti-ebna-1 protein by 12-o-tetradecanoylphorbol-13-acetate treatment of human lymphoblastoid cells. | binding of the epstein-barr virus (ebv) nuclear antigen (ebna-1) to bamhi-c dna was studied by affinity column chromatography followed by immunoblotting with human serum specific for ebna-1. two species of ebna-1 (68 and 70 kilodaltons) were identified in nuclear extracts of the ebv-positive burkitt's lymphoma cell line raji and not in nuclear extracts of the ebv-negative burkitt's lymphoma cell line bjab. both ebna-1s bound specifically to the region required for ebv plasmid dna maintenance (or ... | 1989 | 2545906 |
| production of human heterophile antibodies. iii. in vitro specific stimulation and immortalization of human lymphocytes. | the production of primary human specific antibody against an exogenous antigen by in vitro system was achieved in this study. human lymphocytes were prepared from the lymphocytes of human spleen (spl), tonsil (tl), and peripheral blood (pbl). these lymphocytes were stimulated by sheep red blood cells (srbc) co-cultured with the appropriate number of allogeneic lymphocytes or fractionated t cells. significant numbers of plaque forming cells (pfc) against srbc were obtained. a major population of ... | 1989 | 2545960 |
| [chronic fatigue syndrome associated with epstein-barr virus infection]. | epstein-barr virus (ebv) infection is ubiquitous and may result in multiple and widely different clinical features; the most common of these is infectious mononucleosis (im). recently, a group of patients has been included in the chronic ebv infection syndrome (ebvis), with a sustained nonspecific syndrome consisting of asthenia, anorexia, low grade fever and changes in mood, associated with a viral infection not necessarily caused by ebv; this has been called chronic fatigue syndrome (cfs). we ... | 1989 | 2545980 |
| transmission of epstein-barr virus by a transplanted kidney, with activation by okt3 antibody. | | 1989 | 2546300 |
| analysis of lymphoepithelioma and lymphoepithelioma-like carcinomas for epstein-barr viral genomes by in situ hybridization. | lymphoepithelioma of the nasopharynx has a strong association with epstein-barr virus (ebv). to test the hypothesis that lymphoepithelioma-like carcinomas occurring at other sites are also associated with ebv virus, we used in situ hybridization to analyze 20 cases of lymphoepithelioma and histologically similar lesions and five basaloid squamous cell carcinomas for evidence of ebv genomes. ebv genomes were demonstrated in six of six lymphoepitheliomas of the nasopharynx but in none of five basa ... | 1989 | 2546458 |
| establishment and characterization of an epstein-barr virus transformed cell line with strong phagocytic activity. | an immunoglobulin m (igm)-positive cell line, ms 28, apparently spontaneously transformed by epstein-barr virus (ebv) was established from peripheral blood cells of a patient with immature myeloblastic leukemia. it has been characterized according to phenotype, cytochemistry, and membrane antigen pattern. the cell line expresses lymphoid markers like cd 19, cd 22, and cd 30 and synthesizes and secretes igm. monocyte markers cd 11c, cd 14, and cd 15 are absent. neither interleukin-1 (il-1), nor t ... | 1989 | 2546624 |
| macrophage colony-stimulating factor (csf-1) is expressed by spontaneously outgrown ebv-b cell lines and activated normal b lymphocytes. | human b lymphocytes activated by mitogens or infected by epstein barr virus (ebv) have previously been shown to release colony-stimulating activity (csa) supporting the growth of normal human bone marrow progenitors. we established five different human ebv-b cell lines spontaneously outgrown from nonmalignant peripheral blood cells and long-term bone marrow cultures. csa derived from all of these lines induces the growth of murine macrophage colonies, whereas virtually no human bone marrow cell ... | 1989 | 2546636 |
| [the level of antibodies to epstein-barr virus antigens in the blood serum of the inhabitants of southern siberia and the far east]. | antibodies to epstein-barr virus have been detected in sera from 1538 healthy adult persons living in the south of siberia and far east. it has been shown that the virus infectivity varied from 77% in russians of the yakutsk assr to 100% in orochi and russians residing in the territory of the gorno-altai autonomous province. antibody levels to the epstein-barr virus for inhabitants of the south regions were higher than those for inhabitants of the north regions. | 1989 | 2546730 |
| selective iga deficiency temporally associated with epstein-barr virus infection. | | 1989 | 2547051 |
| infectious mononucleosis and mononucleosis-like illnesses in children and adults in saudi arabia. | the diagnosis of heterophil antibody positive, heterophil negative infectious mononucleosis and mononucleosis-like illnesses as made in 58 children and adult patients with clinical features suggestive of the mononucleosis syndrome and significant number of atypical lymphocytes. epstein-barr virus (ebv) specific serological tests revealed that six children and 23 adults had primary heterophil positive ebv infections based on the detection of igm to eb-viral capsid antigens (igm-vca), presence of ... | 1989 | 2547087 |
| epidemiological studies of nasopharyngeal cancer in the guangzhou area, china. preliminary report. | in the city of guangzhou (canton) and its surroundings nasopharyngeal cancer (npc) constitutes about 32% of all cancer and is therefore the most common kind of cancer. three types of etiological factors seem to be involved: 1) a hereditary factor, 2) epstein-barr virus, and 3) external factors in food and water. an epidemiological study has been started in the guangzhou area with interviews of npc cases, matching controls and mothers of the cases and controls concerning especially food and envir ... | 1989 | 2547276 |
| the clinical spectrum, pathology, and clonal analysis of epstein-barr virus-associated lymphoproliferative disorders in heart-lung transplant recipients. | this study presents the clinical and laboratory observations on posttransplant lymphoproliferative disorders (ptlds) occurring in 5 of 53 heart-lung transplantation recipients. cervical lymph nodes, tonsils, lungs, and gastrointestinal tract were the common sites of involvement by ptlds. the histopathologic findings showed a spectrum of lymphoid and immunoblastic proliferation ranging from diffuse hyperplasia to malignant lymphoma, immunoblastic or large cell type. all cases were associated with ... | 1989 | 2547308 |
| ebv-dna in lymphoid tissue. | | 1989 | 2547311 |
| the epstein-barr virus origin of plasmid replication, orip, contains both the initiation and termination sites of dna replication. | epstein-barr virus (ebv) orip contains two components, a dyad symmetry element and a direct repeat element, that, in the presence of ebv nuclear antigen 1, are necessary and sufficient for plasmid replication. we have examined the replicative forms generated by ebv orip using 2d gel electrophoresis. the patterns obtained from an orip plasmid in a transfected cell line indicate that the site of initiation of dna replication is at or very near the dyad symmetry element, while the direct repeats co ... | 1989 | 2547525 |
| unusual precipitins in pathological human sera. | in conducting double diffusion gel precipitation tests, unexpected, strong precipitation reactions were noticed between a serum originating from a renal graft recipient and many pathological human sera. the highest frequency of positive reactions were produced by sera of patients with infectious mononucleosis (83%), chronic epstein-barr virus infections (72%), rheumatoid arthritis (57%), lepromatous leprosy (57%), and aids (44%). the precipitin in these pathological sera was identified as an ant ... | 1989 | 2547723 |
| il-4 induces lfa-1 and lfa-3 expression on burkitt's lymphoma cell lines. requirement of additional activation by phorbol myristate acetate for induction of homotypic cell adhesions. | lfa-1 and lfa-3 expression is absent or low on burkitt's lymphoma cell lines and low on the ebv-transformed b cell line ud61. incubation of cells of bl2 and of ud61 with various concentrations of il-4 resulted in induction of lfa-1 and lfa-3 expression in a dose dependent fashion. this effect was already observed after 16 h of incubation whereas maximal expression was obtained after 72 h. induction of lfa-1 and lfa-3 expression seemed to be specific for il-4, because il-1, il-2, il-3, ifn-alpha, ... | 1989 | 2547869 |
| chronic active epstein-barr virus (ebv) infection is associated with clonotypic intracellular terminal regions of the ebv. | | 1989 | 2547884 |
| high recombination rate of an epstein-barr virus-simian virus 40 hybrid shuttle vector in human cells. | the stability of an epstein-barr virus (ebv)-simian virus 40 (sv40) hybrid shuttle vector, the p205-gti plasmid, was analyzed in human cells during ebv- or sv40-type replication mode. when the p205-gti plasmid was maintained as an episomal ebv vector in the human 293 cell line, no rearrangement was detected. to induce the sv40 replication mode, cells containing the episomal p205-gti plasmid were either transfected with vectors carrying the t antigen gene or infected with sv40. surprisingly, we o ... | 1989 | 2548000 |
| the epstein-barr virus bmlf1 promoter contains an enhancer element that is responsive to the bzlf1 and brlf1 transactivators. | we have previously shown that the epstein-barr virus (ebv) immediate-early gene product, bzlf1, can activate expression of the ebv bmlf1 immediate-early promoter in ebv-positive, but not ebv-negative, b cells, suggesting that the bzlf1 effect may be mediated through another ebv gene product (s. kenney, j. kamine, e. holley-guthrie, j.-c. lin, e.-c. mar, and j. s. pagano, j. virol. 63:1729-1736, 1989). here, we show that the ebv brlf1 immediate-early gene product transactivates the bmlf1 promoter ... | 1989 | 2548003 |
| [human herpesvirus 6 (hhv-6): incidence in healthy blood donors and in patients with acute infections caused by other herpes viruses]. | antibodies to hhv-6 were detected by immunofluorescence in 8.04% of 460 healthy blood donors in west austria. testing sera from patients with acute or reactivated infections with other herpesviruses, such as cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv) and varicella zoster virus (vzv) we observed a remarkably higher prevalence of antibodies to hhv-6 in patients with cmv infections (75%) and also in patients with ebv infections (50%). patients with hsv and vzv infec ... | 1989 | 2548350 |
| elevated iga antibodies to epstein-barr virus in children with chronic active epstein-barr virus infection. | anti-epstein-barr virus (ebv) antibodies were tested in 11 children with chronic active ebv infection. anti-virus capsid antigen (vca)-igg antibody titers ranged from 1:640 to 1:10,240. anti-vca-igm antibody was consistently positive in 5 of the 11 patients; anti-vca-iga antibody was consistently positive in 6 of the 10 patients; anti-early antigen (ea)-igg antibody was consistently positive in 10 of the 11 patients and anti-ea-iga antibody was consistently positive in 4 out of the 7 patients. a ... | 1989 | 2548373 |
| the establishment of epstein-barr virus nuclear antigen-positive (sp-50b) and epstein-barr virus nuclear antigen-negative (sp-53) cell lines with t(11;14)(q13;q32) chromosome abnormality from an intermediate lymphocytic lymphoma. | two lymphoma cell lines, sp-50b and sp-53, were established from peripheral blood of a 58-year-old woman with leukemic conversion of intermediate lymphocytic lymphoma. these cell lines grew in suspension with or without forming clumps of cells. sp-50b was morphologically similar to the common epstein-barr (eb) virus-transformed lymphoblastoid cell lines and was positive for eb virus nuclear antigen (ebna), whereas sp-53 closely resembled the patient's lymphoma cells and was negative for ebna. bo ... | 1989 | 2548699 |
| heterogeneity of o6-alkylguanine-dna alkyltransferase activity in peripheral blood lymphocytes: relationship between this activity in lymphocytes and in lymphoblastoid lines from normal controls and from patients with hodgkin's disease or non-hodgkin's lymphoma. | we determined o6-alkylguanine-dna alkyltransferase (agt) activity in the peripheral blood lymphocytes (pbls) of normal controls and patients with hodgkin's disease or non-hodgkin's lymphoma and compared these values with those of epstein-barr virus (ebv)-transformed cell lines prepared from the same pbl samples. pbls have an agt level characteristic of the individual from whom the cells were obtained. the agt activity of lymphoblastoid cell lines obtained from a control group of pbls was signifi ... | 1989 | 2548719 |
| [studies on epstein-barr virus strain differences with monoclonal antibodies]. | in order to differentiate a new ebv strain, h18, from other ebv strains, we prepared and screened by indirect immunofluorescence (if) viable cells and fixed cells of a series hybridoma lines that produce monoclonal antibodies against h18 ebv membrane antigen (ma). t1-11 clone was used for further study: t14-11 antibody neutralizes ebv in vitro and recognizes polypeptides p220, p175 and p140 from purified h18 ebv particles, as shown by the western blot technique. the presence of the three polypep ... | 1989 | 2548745 |
| [chronic fatigue symptoms--a new syndrome associated with persistent exhaustion]. | | 1989 | 2548825 |
| immune status towards epstein-barr virus in a group of sicilian children. | the prevalence of antibodies to epstein-barr virus-determined antigens was studied in 17 children with acute infectious mononucleosis (im) and in 263 children hospitalized for diseases unrelated to ebv infection. antibodies against epstein-barr viral capsid antigens (vca) were observed in 173 patients of the control group (66%), but 58 of them (33,5%) had not yet developed antibodies against epstein-barr virus-associated nuclear antigen (ebna). igm-specific antibodies were not found in any of th ... | 1989 | 2548891 |
| [a study on maternal humoral immune responses to villous trophoblast using epstein-barr virus]. | to determine whether or not maternal antibodies are generated during pregnancy, b lymphocytes from puerperal women were transformed using epstein-barr virus (ebv). four weeks after infection with ebv human immunoglobulin (ig) levels were measured in the culture supernatants by elisa. in most wells infected b lymphocytes produced a great amount of ig. the reaction of the culture supernatants from ig-produced wells with placental tissues of their own was examined by immunohistochemically (by an in ... | 1989 | 2549131 |
| comparison of gamma-radiation-induced accumulation of ataxia telangiectasia and control cells in g2 phase. | recent reports from a number of laboratories have linked radiosensitivity in ataxia telangiectasia (a-t) to a large and prolonged block of some cells in g2 phase. previous results from this laboratory, largely with one epstein-barr virus-transformed a-t lymphoblastoid cell line, presented evidence for a dramatic increase in the number of cells in g2 phase over controls during a 24-h period post irradiation. we describe here a study of the effect of gamma-radiation on g2 phase delay in several a- ... | 1989 | 2549409 |
| [in situ hybridization in detecting virus infections in biopsies following orthotopic liver transplantation]. | using the in-situ dna hybridization we studied 62 liver biopsies from 34 patients for the presence of cmv-, ebv-, hbv-, and hsv-genome. in the first biopsies positivity-rates were as follows: cmv 62%, ebv 71%, hbv 15%, hsv 15%. in 6 cases a conversion from a negative intranuclear cmv-status to a positive status was seen correlating with an increase in serological titers and with clinical signs of viral infection. | 1989 | 2549570 |
| epstein-barr virus (ebv). vii. established lymphoid cell line (ivpat-88) obtained from synovial fluid of a patient with aseptic arthritis. | attempts have been made to culture mononuclear cells from synovial fluid of 8 patients with arthropathy, and have led to the development of the lymphoid cell line ivpat-88. cell line has been propagated by serial passages for more than 14 weeks in continuous culture. the cells grew as single, free-floating individuals, or in dense clumps without adherence to glass or plastic surface. all these cells were identified as altered lymphoblasts because of their growth pattern and uniform morphology, a ... | 1989 | 2549701 |
| agranulocytosis and anaemia induced by sulfametopyrazine in a sulfametopyrazine-trimethoprim combination. | we report a case of prolonged fever, agranulocytosis, and anaemia associated with the long acting sulphametopyrazine-trimethoprim combination (kelfiprim). a woman of 23 years took an overdose of 13 tablets over five days for presumed cystitis. one day after the last dose the patient developed fever and a generalised rash. the fever persisted and her previously normal leukocyte count decreased to 1.8 x 10(9)/1. after treatment with paracetamol the fever settled briefly, and then recurred for anot ... | 1989 | 2549841 |
| metabolism of pyrenedecanoic acid in epstein-barr virus-transformed lymphoid cell lines from normal subjects and from a patient with multisystemic lipid storage myopathy. | a lymphoid cell line has been established from a patient with multisystemic lipid storage myopathy and showed a major triacylglycerol storage, whereas the content of other neutral lipids and phospholipids was in the normal range. the metabolism of the triacylglycerols has been investigated in this lymphoid cell line from multisystemic lipid storage myopathy as well as in control cells through pulse-chase experiments using 10-(1-pyrene)decanoic acid (p10), a fluorescent fatty acid derivative, as ... | 1989 | 2550076 |
| [study on human monoclonal antibodies in hemorrhagic fever with renal syndrome virus]. | | 1989 | 2550116 |
| lysis of p3hr-1 cells induced to enter the viral cycle by antibody-dependent and independent immunological mechanisms. | the p3hr-1 burkitt lymphoma line carries the epstein-barr virus (ebv) genome and a small proportion of the cells (1-3%) enter the lytic cycle spontaneously. treatment with tpa and n-butyrate elevates considerably the number of virus-producing cells (25-35%). cells which enter the lytic cycle express the ebv early antigen ea, the viral capsid antigen vca, and the membrane antigen ma. antibodies against these antigens are present in ebv-immune human sera. the expression of virus envelope protein o ... | 1989 | 2550147 |
| efficient synthesis of influenza virus hemagglutinin in mammalian cells with an extrachromosomal epstein-barr virus vector. | the capability of an epstein-barr virus hybrid vector (ebv-cmv), containing the cytomegalovirus (cmv) immediate early enhancer and simian virus 40 promoter, to produce large amounts of authentic mammalian proteins was studied. the cdna of influenza virus hemagglutinin (ha), a cell surface glycoprotein, was inserted into this vector and the ebv-cmv-ha plasmid was transfected into two human and two monkey cell lines. southern-blot analysis revealed that the ebv-cmv-ha plasmid was maintained in ext ... | 1989 | 2550325 |
| replication of latent epstein-barr virus genomes in normal and malignant lymphoid cells. | dna replication of 2 human lymphoid cell lines (u296 and raji), latently infected with the epstein-barr virus, has been compared using a density transfer approach. typical of non-malignant lymphoblastoid cells, u296 cells divided once in bromodeoxyuridine-supplemented medium to form hybrid but not heavy-density host dna. replication of the intracellular epstein-barr virus dna was selectively inhibited in these cells with only 15% of the viral genomes duplicating once to form hybrid-density viral ... | 1989 | 2550376 |
| epstein-barr virus replication in interferon-treated cells. | the burkitt's lymphoma-derived cell line daudi is latently infected with the epstein-barr virus (ebv). these cells are very sensitive to the growth inhibitory and differentiation-inducing effects of human interferon-alpha (ifn) and we have examined the possibility that these responses are due to induction of ebv replication. our results indicate that only a very small proportion of cells (less than 0.3%) are induced by ifn treatment to express ebv lytic genes at the rna or protein level, whereas ... | 1989 | 2550580 |
| epstein-barr virus (ebv)-containing nasopharyngeal carcinoma cells express the b-cell activation antigen blast2/cd23 and low levels of the ebv receptor cr2. | anaplastic nasopharyngeal carcinoma (npc) cells invariably harbor the epstein-barr virus (ebv) genome, an association that is unique among human virus-associated cancers. although ebv is able to replicate in epithelial cells, results with expression of the ebv receptor (complement receptor type 2 [cr2]; also called cd21) in normal and malignant epithelial cells are conflicting. we grew five different ebv-associated npc tumors in nude mice, and by using a sensitive transcriptional assay, we detec ... | 1989 | 2550660 |
| in situ detection of epstein-barr virus markers in nasopharyngeal carcinoma patients. | cryosections of nasopharyngeal tissue from 9 patients suspected of having nasopharyngeal carcinoma (npc) were examined for the presence of epstein-barr virus (ebv) markers in situ to assess virus infection in the nasopharynx. viral dna, eb nuclear antigen, and/or early antigens (ea.d and ea.r) were detected in 5 npc specimens. ebv infection was not confined to the tumor areas of the biopsy specimens. lymphoid cells and nontumor areas of these specimens contained ebv markers. in addition, nasopha ... | 1989 | 2550863 |
| possible involvement of epstein-barr virus (ebv) in polyclonal b-cell activation in sjögren's syndrome. | the following results suggest that ebv might be involved in the mechanism of polyclonal b-cell activation of sjögren's syndrome (ss). (1) the levels of serum anti-vca antibodies of both igg and igm class were significantly elevated in ss. (2) excretion of ebv from the oropharynx was frequently observed in ss. (3) spontaneously transformed b-cell lines producing a large amount of transforming ebv were established preferentially from ss. (4) an ebv-specific regulatory mechanism was impaired in ss. | 1989 | 2551309 |
| epstein-barr virus dna replication. | | 1989 | 2551584 |
| post-transcriptional mechanisms of deregulation of myc following conversion of a human b cell line by epstein-barr virus. | by utilizing an epstein-barr virus (ebv)-negative burkitt's lymphoma line (bjab) and several ebv-positive sub-lines derived from it by in vitro infection, it has been shown previously that the presence of the ebv genome in this b cell line is reversibly associated with deregulation of myc expression. specifically, the decline in the level of myc transcripts observed in the ebv-negative bjab line as cells approach stationary phase of growth is abrogated in the presence of the virus. in the studie ... | 1989 | 2551670 |
| immunological status to epstein-barr virus and cytomegalovirus in patients with genital condylomata. | serological patterns against epstein-barr virus (ebv) and cytomegalovirus (cmv) were determined in patients with genital condylomata (gc). the ig g antibody values to eb-induced virus capsidic antigens (vca), early antigens (ea) and ig m to vca were significantly higher in the study group than in the controls. moreover, the concomitant presence of ebv-ig g anti-vca greater than or equal to 1/320, ebv-ig g anti-ea greater than or equal to 1/20 and ebv-ig m anti vca greater than or equal to 1/20 w ... | 1989 | 2551722 |
| rearranged epstein-barr virus genomes and clonal origin in nasopharyngeal carcinoma. | epstein-barr virus (ebv) is known to be associated with two malignant diseases, nasopharyngeal carcinoma (npc) and endemic burkitt's lymphoma. in this study, the genomes of ebv in biopsy specimens from 4 npc patients in japan were analyzed using southern blot hybridization. the npc tissues of all examined cases contained rearranged ebv genomes whose bamhi h fragments were larger than those of prototype ebv genomes. one of them had a bamhi fragment containing contiguous sequences of bamhi y and h ... | 1989 | 2551868 |
| human herpesviruses: a consideration of the latent state. | | 1989 | 2552271 |
| [molecular mechanisms of establishment and disruption of epstein-barr virus latency]. | | 1989 | 2552500 |
| recent advances on epstein-barr virus infectious mononucleosis. | infectious mononucleosis (im) is an acute, self-limited lymphoproliferative disorder caused by ebv. the classic features consist of fever, malaise, easy fatigability, pharyngotonsillitis, cervical lymphadenopathy, splenomegaly, subclinical hepatitis, and atypical lymphocytosis. symptomatic im occurs in older children and adolescents, whereas the subclinical im is the rule in toddlers and young children. in general the prognosis is good, even in the more prolonged and serious cases. the laborator ... | 1989 | 2552512 |
| malaria, epstein-barr virus, and the genesis of lymphomas. | | 1989 | 2552758 |
| the genomes of the human herpesviruses: contents, relationships, and evolution. | | 1989 | 2552898 |
| telomeric associations in a lymphoblastoid cell line from a patient with b-cell follicular lymphoma. | we have established a new epstein-barr virus transformed cell line from a patient with b-cell follicular lymphoma. telomeric fusions were observed in several subclones, with the nonrandom involvement of chromosomes 1, 5, 12, and 17. centromeric staining with immunofluorescent anti-kinetochore antibodies was positive in both centromeres of the fused chromosomes, suggesting they were both active. unlike previously reported cases, we were unable to demonstrate telomeric fusions directly in cells fr ... | 1989 | 2553339 |
| hepatitis b virus surface antigen as a reporter of promoter activity. | the coding sequence for the hepatitis b virus surface antigen (hbsag) was used as a new reporter gene for studies on eukaryotic promoter activity and upstream regulatory sequences. the data observed in transfection assays were comparable to results obtained with conventional chloramphenicol acetyltransferase (cat) assays, as was demonstrated using various transcriptional regulation sequences. the expression of hbsag as a reporter protein offered some advantages: (i) in transient expression assay ... | 1989 | 2553536 |
| [malignant lymphoma which contained ebv dna and lacked ebna2 antibody]. | a case of non-hodgkin malignant lymphoma which contained ebv dna and ebna was reported. the serum of this patient showed high titer of vca, ea, and ebna1 antibody but lacked ebna2 antibody. the relation with ebv originated malignancy and defect of ebv specific antibody suggests a process of oncogenicity of this virus. | 1989 | 2554014 |
| the epstein-barr virus and nasopharyngeal carcinoma. | | 1989 | 2554089 |
| characteristics of epstein-barr virus-carrying lymphoblastoid cells derived from human tonsil: ebv production and autoantibody response. | tonsillar and peripheral blood lymphocytes from 20 patients with tonsillitis were examined for production of epstein-barr virus (ebv) and antinuclear antibodies within 2 weeks after ebv transformation. all established lymphoblastoid cell lines (lcls) contained more than 90% ebv nuclear antigen-positive cells. however, no lcls produced biologically active virus. on the other hand, 10 (50%) out of 20 tonsillar lcls and 4 (20%) out of 20 peripheral blood lcls produced autoantibodies. these results ... | 1989 | 2554232 |
| is the epstein-barr virus involved in hodgkin's disease? | ebv genomes, in clonal episomal form, were detected in 7 out of 17 cases of hodgkin's disease (hd) and in a single case of non-hodgkin's lymphoma which occurred in a patient after therapeutic treatment for hd. the experimental data presented imply that a clonal cell population, harboring the ebv genome, must be present in ebv-positive hd. in light of this finding we are attempting to reconsider the abundant literature on this lymphoproliferative disorder, and suggest a reevaluation of the possib ... | 1989 | 2554550 |
| [biology of the epstein-barr virus]. | epstein-barr virus (ebv) displays several biological properties which distinguish it from any other human herpesvirus. the most important of these is that it infects and immortalizes human b lymphocytes, both in vitro and in vivo. these indefinitely growing cells consistently express viral proteins in the nucleus or in the plasma membrane; at least one of these is required for immortalization. in severely immunocompromised hosts such as transplant recipients and aids patients, ebv induces b-cell ... | 1989 | 2554764 |
| [macrophage activation syndrome linked to epstein-barr virus]. | epstein-barr virus infection is one of the etiologies that should be discussed in patients with macrophage activation syndrome. we report a case that is consistent with this diagnosis. the role of the epstein-barr virus in the etiologic diagnosis of vahs (virus-associated hemophagocytic syndrome) is specified. in pediatric patients with vahs, the other causes of macrophage activation should also be discussed. | 1989 | 2554773 |
| a transforming function of the barf1 gene encoded by epstein-barr virus. | we report a new rodent cell-transforming gene, presumably involved in viral replication, encoded by epstein-barr virus. we previously showed that the corresponding open reading frame barf1 is transcribed before the onset of viral dna synthesis, and translated into a 33 kd early polypeptide (p33). here we show that recombinant plasmids containing the barf1 induce morphological change, anchorage-independent growth and tumorigenic transformation of established mouse fibroblast lines. the barf1-tran ... | 1989 | 2555151 |
| molecular cloning of human uracil-dna glycosylase, a highly conserved dna repair enzyme. | uracil-dna glycosylase is the dna repair enzyme responsible for the removal of uracil from dna, and it is present in all organisms investigated. here we report on the cloning and sequencing of a cdna encoding the human uracil-dna glycosylase. the sequences of uracil-dna glycosylases from yeast, escherichia coli, herpes simplex virus type 1 and 2, and homologous genes from varicella-zoster and epstein-barr viruses are known. it is shown in this report that the predicted amino acid sequence of the ... | 1989 | 2555154 |
| [kawasaki disease associated with epstein-barr virus]. | we describe a 14-month-old child who for 3 weeks had high fever and developed lesions of the mucous membranes, enlarged lymph nodes, and an erythematous rash. kawasaki disease was diagnosed and there was clear evidence for infection with epstein-barr virus. thus it is possible that this virus is one of the etiological factors in the development of kawasaki disease. | 1989 | 2555288 |
| hydrodynamic, electron microscopic, and ligand-binding analysis of the epstein-barr virus/c3dg receptor (cr2). | the interaction of the epstein-barr virus/45-kda proteolytic fragment of c3 (c3dg) receptor (cr2) with its viral ligand, the epstein-barr virus glycoprotein gp350/220, initiates the sequence of events leading to virus internalization and b lymphocyte transformation. soluble recombinant receptor (rcr2) and gp350/220 as well as the natural ligand, c3dg, were subjected to a number of analytical techniques including gel permeation chromatography, density gradient ultracentrifugation, circular dichro ... | 1989 | 2555366 |
| the terminal protein gene 2 of epstein-barr virus is transcribed from a bidirectional latent promoter region. | the intact terminal protein genes (tp1 and tp2) of epstein-barr virus (ebv) are created upon infection by circularization of the linear viral genome at its terminal repeats. the structure of the 1.7 kb tp2 latent mrna has been determined by cdna analysis and northern blotting, revealing its close relation to tp1 mrna. the 1.7 kb transcript is expressed from a different promoter and has a different 5' exon from tp1 but is also spliced across the terminal repeats. the last eight exons are common t ... | 1989 | 2555438 |
| seroepidemiologic studies of cytomegalovirus and epstein-barr virus infections in relation to human immunodeficiency virus type 1 infection in selected recipient populations. transfusion safety study group. | antibodies to human cytomegalovirus (cmv) and epstein-barr virus (ebv) were evaluated among 1,171 persons with and without antibodies to human immunodeficiency virus type 1 (anti-hiv-1). these included 97 blood donors, 577 persons given blood components or products, and 497 controls. a significantly higher proportion of anti-hiv-1 positive than -negative donors were anti-cmv-positive, a finding associated with homosexual contact among some of the former. among subjects with treated clotting diso ... | 1989 | 2555471 |
| the epstein-barr virus (ebv) early protein eb2 is a posttranscriptional activator expressed under the control of ebv transcription factors eb1 and r. | from the cloning and characterization of cdnas, we found that the epstein-barr virus (ebv) open reading frame (orf) bmlf1-bslf2 coding for the early protein eb2 is present in several mrnas generated by alternative splicing and expressed in the leftward direction from two promoters pm and pm1. the pm promoter controls the expression of two abundant mrna species of 1.9 and 2 kilobases (kb), whereas the pm1 promoter controls the expression of at least three mrnas 3.6, 4.0, and 4.4 kb long. the pm p ... | 1989 | 2555554 |
| intravenous immunoglobulin treatment of epstein-barr virus-related idiopathic thrombocytopenic purpura. | | 1989 | 2556233 |
| epstein-barr virus nuclear protein 2 is a key determinant of lymphocyte transformation. | epstein-barr virus (ebv) efficiently transforms b lymphocytes to perpetual proliferation. the ebv laboratory strain p3hr-1 is transformation-incompetent and lacks a dna segment that includes the ebv nuclear antigen 2 (ebna-2) gene and a portion of the ebna leader protein (ebna-lp) gene. these two genes are expressed in transformed b lymphocytes. recombinant transformation-competent ebvs were produced by transfecting p3hr-1-infected cells with a cosmid containing the dna deleted in p3hr-1. deleti ... | 1989 | 2556717 |
| characterization of human ifn-gamma response using somatic cell hybrids of hematopoietic and nonhematopoietic origin. | a panel of 27 rodent-human somatic cell hybrids composed of cells of hematopoietic (nonadherent cells) and nonhematopoietic origin (adherent cells) was used to identify the chromosomes involved in the biological response to human ifn-gamma (hu-ifn-gamma). we found that the stimulation of class-i histocompatibility antigen expression correlates with the presence of human chromosomes 6 and 21 in adherent cell hybrids, while human chromosome 6 alone is sufficient in nonadherent hybrids. scatchard a ... | 1989 | 2556800 |
| size and stability of the epstein-barr virus major internal repeat (ir-1) in burkitt's lymphoma and lymphoblastoid cell lines. | we have used field inversion gel electrophoresis to survey ebv strains for the size of the major internal repeat, ir-1, and estimate the number of 3.1-kb repeat units present. the b95-8 strain of ebv was estimated to contain 8.6 repeats. the repeat number varies considerably among naturally occurring isolates around a mean of six repeats. some cell lines harbored multiple viral genomes with differing numbers of repeats and our results suggest that the repeat number in ir-1 is more likely to chan ... | 1989 | 2556840 |
| disparate effects of two herpesvirus [corrected] immediate-early gene trans-activators on the hiv-1 ltr. | the bmlf1 region of the epstein-barr virus (ebv) genome and the immediate-early (ie) region of human cytomegalovirus (hcmv) both encode proteins which can trans-activate heterologous promoter/chloramphenicol acetyl transferase (cat) constructs, including a human immunodeficiency virus type-1 promoter/cat construct. we demonstrate here that this trans-activation by the ebv bmlf1 gene product, which we have previously shown to be largely post-transcriptional, is reporter gene dependent. in contras ... | 1989 | 2556854 |
| [immunological aspects in anti-hiv seronegative drug addicts. correlation with the duration of heroin addiction and other viral infections]. | in order to determine whether the immunological abnormalities described in intravenous drug addicts (ida), are due to hiv infection, other viral infections or to the abuse of narcotic drugs, we studied the t lymphocyte subsets and serological markers of infection with hepatitis b and delta virus, cytomegalovirus and epstein barr virus, in 49 ida. the immunological and serological features of ida were compared with the control group, made up of 20 healthy subjects. in intravenous drug abusers we ... | 1989 | 2557564 |
| cpg island clones from a deletion encompassing the gene for adenomatous polyposis coli. | adenomatous polyposis coli (apc), a dominantly inherited disorder, has been mapped to chromosome 5q15-q21 by family linkage studies. cells from patients with deletions in this region, in one case associated with polyposis in a family, have been used to construct human hamster hybrid cell lines that retain either the normal or deleted chromosome 5. these lines have been used to identify markers from the region of the polyposis gene obtained by cloning the ends of 0.5- to 2-megabase bsshii fragmen ... | 1989 | 2557613 |
| an ochre mutation in the vitamin d receptor gene causes hereditary 1,25-dihydroxyvitamin d3-resistant rickets in three families. | hereditary 1,25-dihydroxyvitamin d3-resistant rickets is a rare autosomal-recessive disease resulting from target-organ resistance to the action of the active hormonal form of vitamin d. four affected children from three related families with the classical syndrome of hereditary 1,25-dihydroxyvitamin d3-resistant rickets and the absence of detectable binding to the vitamin d receptor (vdr) in cultured fibroblasts or lymphoblasts were examined for genetic abnormalities in the vdr gene. genomic dn ... | 1989 | 2557627 |
| transforming activity of nasopharyngeal carcinoma dna detectable in mouse jb6 cells. | a jb6 mouse epidermal recipient cell line has been used to detect nasopharyngeal carcinoma (npc) dna-associated transforming activity that is not detectable in the nih 3t3 focus assay. npc dna showed both transforming activity and activity for transferring sensitivity to tumor-promoter-induced neoplastic transformation, assayed in 2 different variants of mouse jb6 cells. comparison of dnas from various npc sources that did or did not harbor ebv dna and that varied in degree of differentiation sh ... | 1989 | 2558076 |
| volatile nitrosamine levels and genotoxicity of food samples from high-risk areas for nasopharyngeal carcinoma before and after nitrosation. | traditional life-style, especially food habits, infection by epstein-barr virus (ebv) and genetic factors, have been associated with an increased risk of nasopharyngeal carcinoma (npc). n-nitroso compounds and other carcinogens either present in food or formed endogenously, as well as food constituents that activate ebv, have been suspected as etiological factors in npc pathogenesis. for their characterization preserved food items, frequently consumed in npc endemic areas in tunisia, south china ... | 1989 | 2558079 |
| explants of human oral epithelium exposed to viruses and cancer chemotherapeutics. | cultures of proliferating epithelial cells were established from explants of normal human oral epithelium from healthy young volunteers. the epithelial cells were found permissive for herpes simplex virus type 1 and type 2, coxsackie virus a-4 and a-16, adenovirus type 5, measles vaccine, rubella and influenza type a virus-. medium from deae-pretreated epithelial cultures infected with two subtypes of human immunodeficiency virus-1 showed an increasing content of virusprotein with time by antige ... | 1989 | 2558179 |
| recurrent/persistent pneumonia in a 3 1/2-year-old-girl due to acquired immune deficiency syndrome. | | 1989 | 2558921 |
| isolation of igg islet cell autoantibody-producing b lymphocytes from the peripheral blood of type 1 diabetic patients and an ica-positive non-diabetic individual. | | 1989 | 2559015 |
| what's new in human herpesvirus-6? clinical immunopathology of the hhv-6 infection. | human herpesvirus-6 (hhv-6), formerly known as human b-lymphotropic virus (hblv), was first isolated in 1986 from patients with lymphoproliferative disorders and aids. antibody prevalence against hhv-6 varies between about 60-80% indicating a widespread latent infection. although hhv-6 infects in vivo primarily t-lymphocytes, it is associated with similar diseases as in infection with epstein-barr virus (ebv), a clearly b-lymphotropic virus. reactivation of latent hhv-6 infection in patients wit ... | 1989 | 2559396 |
| characterization of a 75-kda epstein-barr virus capsid protein using a new monoclonal antibody h250. | a monoclonal antibody (mab) designated h250, directed against an epstein-barr virus (ebv) capsid antigen, was obtained following immunization of balb/c mice with naked particles from the producer cell line b95.8. this antigen was present in the producer lines b95.8, p3hr1, m81, ri and ca, and absent from the non-producer lines bjab, raji and 1022. h250 did not inhibit the transformation of cord blood lymphocytes by the b95.8 virus, nor did it inhibit ea induction on raji cells by the p3hr1 virus ... | 1989 | 2559442 |
| cell-mediated immunity in infectious mononucleosis. | | 1989 | 2559613 |
| epstein-barr-virus-related post-bone-marrow-transplant lymphoproliferative disease: association with cytomegalovirus infection and down syndrome donor marrow. | we describe the development of epstein-barr-virus (ebv)-related lymphoproliferative disease (lpd) in the recipient of a histocompatible bone marrow transplant (bmt). although this rare complication is more common in recipients of mismatched bone marrow, several distinguishing features of our case may have contributed to the development of lpd in the recipient of a matched bone marrow transplant. the patient had received marrow from a sibling with trisomy 21, a syndrome associated with variable c ... | 1989 | 2559622 |
| [optic neuropathy in purtilo's syndrome]. | | 1989 | 2559817 |
| production of carbohydrate-specific human monoclonal antibodies in vitro. | | 1989 | 2560123 |
| hairy leukoplakia, ebv: an oral history of aids. | | 1989 | 2561057 |
| immunotherapy and enhanced antibody-dependent cell-mediated cytotoxicity using virally-infected target cells. | we examined the ability of in vitro addition of interleukin-2 (il-2) to differentially enhance antibody-dependent cell mediated cytotoxicity (adcc) utilizing cultured epstein-barr virus infected cells and gammaglobulin (sandoglobulin). we found significant enhancement of adcc when il-2 was added. chronic epstein-barr virus or chronic fatigue syndrome patients in a therapeutic gammaglobulin program may benefit from il-2 given in vivo. | 1989 | 2561291 |
| sjögren's syndrome. | | 1989 | 2561729 |
| [cluster headache, epstein-barr and burkitt's lymphoma virus infections]. | thirteen patients affected by burkitt's lymphoma were studied. six out of them had cluster headache associated with burkitt's lymphoma, and in this group we observed the absence of hla-b14 antigen. the presence of hla-b14 antigen in the remaining seven patients, confirm the hypothesis of a protective role of this antigen against cluster headache. | 1989 | 2561749 |
| a kinetic analysis of lymphocyte 5'-nucleotidase in b-cell clones from control subjects and patients with primary hypogammaglobulinaemia. | the affinity of lymphocyte 5'-nucleotidase (5nt) for its substrate adenosine monophosphate (amp) was studied in epstein-barr virus immortalized b-lymphocyte clones from healthy controls and patients with primary hypogammaglobulinaemia. km values for amp for 5nt in most of the patient clones were found to be significantly increased compared to b-cell clones from normal subjects, whereas vmax values were within the normal range. | 1989 | 2561998 |
| forward and reverse changes in ig/myc translocation carrying tumors. | translocation of the c-myc protooncogene to an immunoglobulin locus is a rate-limiting step in the genesis of three b cell derived tumors: burkitt lymphoma (bl) in humans, mouse plasmacytoma (mpc), and rat immunocytoma (ric). the translocation appears as a rate-limiting step in the genesis of all three tumors. its consequences have been best analyzed in bl. they involve a non-immunological and an immunological component. the former acts by preventing the b cell from leaving the cycling compartme ... | 1989 | 2562178 |
| evidence for differential expression of cd45 isoforms by precursors for memory-dependent and independent cytotoxic responses: human cd8 memory ctlp selectively express cd45ro (uchl1). | on the basis of differential cd45 expression, human t cells can be separated into approximately reciprocal populations. the mab uchl1 detects a 180 kd molecular mass isoform, cd45r0. the cd45ra cluster of mabs reacts with cd45 isoforms of 205 and 220 kd molecular mass. these reagents subdivide both cd4 and cd8 t cell populations. cd4 t cells that proliferate in response to memory-dependent (recall) antigens have been shown to selectively express cd45r0. we extend these observations to a model fo ... | 1989 | 2562244 |
| molecular biology of lymphoid malignancies. | | 1989 | 2562284 |
| [evaluation of serological criteria for the diagnosis of infectious mononucleosis]. | we have evaluated the application for the diagnosis of infectious mononucleosis of several serologic criteria, including measurement of concentrations of igm against viral capsid antigen (vca) of epstein-bar virus (ebv) by elisa, detection of heterophil antibodies and the presence of anti-vca igg in absence of igg against nuclear antigen of ebv (ebna). such criteria were evaluated by using a board of sera obtained from cases who had clinically been diagnosed of infectious mononucleosis and ident ... | 1989 | 2562304 |
| role of different lymphocyte subsets in human anti-viral t cell cultures. | we have systematically studied uncloned human cell lines derived from anti-influenza a virus or anti-epstein-barr virus (ebv) bulk cultures, or from cultures highly enriched for cd4+ or cd8+ lymphocytes. the most noteworthy results are the following: (1) anti-viral bulk cultures consisted of more than 90% of cd8+ cells in all cases. in contrast, anti-hla cell lines are composed of approximately 50% cd8+ and 50% cd4+ cells. all of the cd8+ and cd4+ cells present in the culture were also 4b4+/2h4- ... | 1989 | 2562930 |