| management of patients with mildly dysplastic cervical smears. | the management of patients with mild dysplasia on cervical smears remains controversial. recent studies have shown that many harbor high grade squamous intraepithelial lesions (hsil) and human papillomavirus (hpv) and warrant further evaluation rather than follow-up. this prospective study dealt with the evaluation of patients with mild dysplastic smears using colposcopy and directed biopsies with view to identifying hsil/hpv and treating them. among 100 cases studied, 61% revealed hsil/hpv; of ... | 1994 | 7932399 |
| allelic loss at chromosomes 3p, 8p, 13q, and 17p associated with poor prognosis in head and neck cancer. | little is known about the molecular genetic events that contribute to the pathogenesis of squamous cell carcinoma of the upper aerodigestive tract. previous molecular genetic studies have been limited to the identification of mutations of the p53 (also known as tp53) tumor suppressor gene, activation of a limited set of oncogenes, allelic loss at 3p and other locations, and occasional association with human papillomavirus infection. | 1994 | 7932807 |
| interaction of papillomaviruses with the cell surface. | to initiate an investigation of the initial step in papillomavirus infection, we have examined the interaction of bovine papillomavirus type 1 (bpv) virions with c127 cells by two assays, binding of radioiodinated bpv virions to cell monolayers and bpv-induced focal transformation. under physiological conditions, the labeled virions bound to the cell surface in a dose-dependent manner within 1 h. antibody studies indicated that the interaction was specific and related to infectivity: polyclonal ... | 1994 | 7933109 |
| transitional cell carcinoma of the bladder: failure to demonstrate human papillomavirus deoxyribonucleic acid by in situ hybridization and polymerase chain reaction. | human papillomaviruses have been implicated in the pathogenesis of a variety of malignancies, particularly those of the anogenital tract. some recent reports on the presence of human papillomavirus in bladder cancer have raised the possibility that it might be involved in the development of this malignancy as well. to study this concept, a series of 108 transitional cell carcinomas of the bladder were screened for the presence of human papillomavirus deoxyribonucleic acid (dna) by in situ hybrid ... | 1994 | 7933176 |
| localisation of hiv-1 to human papillomavirus associated cervical lesions. | | 1994 | 7934364 |
| relation between human papillomavirus type 16 and potential for progression of minor-grade cervical disease. | | 1994 | 7934482 |
| the retinoblastoma protein-binding region of simian virus 40 large t antigen alters cell cycle regulation in lenses of transgenic mice. | regulation of the cell cycle is a critical aspect of cellular proliferation, differentiation, and transformation. in many cell types, the differentiation process is accompanied by a loss of proliferative capability, so that terminally differentiated cells become postmitotic and no longer progress through the cell cycle. in the experiments described here, the ocular lens has been used as a system to examine the role of the retinoblastoma protein (prb) family in regulation of the cell cycle during ... | 1994 | 7935393 |
| a non-radioactive assay for the detection and quantitation of a dna binding protein. | | 1994 | 7937095 |
| nuclear localization and transforming activity of human papillomavirus type 16 e7-beta-galactosidase fusion protein: characterization of the nuclear localization sequence. | a mab 9f6 was capable of staining hpv16 e7 in a human cervical carcinoma line, caski, and rat 3y1 cells stably expressing hpv16 e7 gene. contrary to the current understanding of e7 as a nuclear protein, the site of staining was clearly cytoplasmic. the subcellular localization of e7 was further studied by using the beta-galactosidase (beta-gal) receptor method. a fusion protein composed of e7 and beta-gal was stably expressed in rat 3y1 cells. the beta-gal activity in these cells was detected mo ... | 1994 | 7941347 |
| transformation of common warts into squamous cell carcinoma on sun-exposed areas in an immunosuppressed patient. | we report one case of renal transplant recipient who developed widespread multiple verrucous skin lesions. histologic examination revealed typical warts and, only from several sites exposed to sun (hands and face), a development of dysplasia within the warts and a transformation of some of them toward infiltrating squamous cell carcinoma (scc). human papillomavirus (hpv) testing for dna by polymerase chain reaction dna amplification identified hpv type 1 in both warts and scc. our findings sugge ... | 1994 | 7949492 |
| determinants of human papillomavirus types 16 and 18 infections in the lower female genital tract in an italian population. | the prevalences of human papillomavirus (hpv) types 16 and 18 infection were determined by a non isotopic molecular hybridization assay on cervical scrapes from 738 women affected by gynaecological lesions different from malignancies. the correlation with known epidemiological risk factors for cervical neoplasia, such as sexual habits, smoking and pill use, was investigated. the overall hpv prevalence rate was 29.8% (220/738). viral dna sequences were detected in 26.9% (122/452) of morphological ... | 1994 | 7957325 |
| long-term genome stability and minimal genotypic and phenotypic alterations in hpv16 e7-, but not e6-, immortalized human uroepithelial cells. | parameters of genome instability and morphological alterations associated with cell transformation were studied in an isogeneic set of clonal human uroepithelial cell (huc) lines immortalized by the human papilloma virus 16 (hpv16) e6 and/or e7 gene(s). hpv16 e6 binds p53, leading to rapid degradation of p53, whereas e7 binds and alters prb and other proteins. we report that two independent e7-immortalized huc lines showed minimal phenotypic or genotypic alterations, except that both lines conta ... | 1994 | 7958891 |
| vaginal micropapillary lesions are not related to human papillomavirus infection: in situ hybridization and polymerase chain reaction detection techniques. | the objective of this study was to assess the human papillomavirus dna presence in vaginal papillary lesions, with particular regard to micropapillomatosis to better define their clinical significance. prospective study: the study population was composed of 62 women who were recruited consecutively from the colposcopy centre of the ancona university, department of obstetrics and gynecology, on the grounds of vaginal papillomatosis or/and typical acuminata warts. biopsies for routine histology, a ... | 1994 | 7959342 |
| diagnosis of genital human papillomavirus lesions in the male. | | 1994 | 7959721 |
| pathogenetic significance of p53 and c-ki-ras gene mutations and human papillomavirus dna integration in adenocarcinoma of the uterine cervix and uterine isthmus. | the pathogenetic significance of p53 and c-ki-ras gene mutations and genomic integration of human papillomavirus (hpv) dna was examined in surgically resected specimens of adenocarcinomas of the uterine cervix and isthmus using polymerase chain reaction (pcr), single-strand-conformation polymorphism and southern blotting analysis. among 25 cervical adenocarcinomas, p53 gene mutations between exons 5 and 8 were detected in 32%, and the incidence of these mutations was higher in cases at advanced ... | 1994 | 7960232 |
| pcr in situ hybridisation detection of hpv 16 in fixed caski and fixed siha cell lines. | to investigate the feasibility of using fixed cells with the polymerase chain reaction (pcr) in situ hybridisation and to investigate possible reasons for reaction failure. | 1994 | 7962608 |
| prevalence of human papillomavirus types 16 and 18 in penile carcinoma: a study of 41 cases using pcr. | to determine retrospectively the prevalence of human papillomavirus (hpv) types 16 and 18 in penile carcinomas. | 1994 | 7962651 |
| epidemiology and partial nucleotide sequence of four novel genital human papillomaviruses. | polymerase chain reaction (pcr)-based genital human papillomavirus (hpv) detection methods that use consensus primers have enabled the broad-spectrum detection of most characterized hpv types. in addition, these techniques have allowed the identification of potentially novel viral sequences in clinical specimens. these methods were used to determine the partial l1 nucleotide sequence (the region generated by l1 consensus primers my09 and my11) of four novel viruses. the prevalence of these virus ... | 1994 | 7963700 |
| epithelial alterations adjacent to invasive squamous carcinoma of the vulva. | the slides of 64 vulvectomy specimens from vulvar squamous carcinoma were reviewed in order to study the histopathologic changes adjacent to the neoplasia. normal epithelium was found in 7 cases (11%) and epithelial alterations adjacent to carcinoma in 59 (89%). the epithelial alterations found were: nonneoplastic epithelial disorder (nndv) in 38 cases (59%) and vulvar intraepithelial neoplasia (vin) in 19 (30%). the distribution of nndv was: 20 cases of epithelial hyperplasia (eh) (31%), 6 of l ... | 1994 | 7966043 |
| treatment of urethral condylomata acuminata or flat condylomata with interferon-alpha 2a. | the effectiveness of intraurethral 5-fluorouracil and systemic administration of interferon-alpha 2a in the therapy of condylomata acuminata or flat condylomata of the urethra is assessed. from march 1986 to september 1991, 1,372 male sexual partners of women with condylomata acuminata, flat condylomata or cervical intraepithelial neoplasia underwent urethroscopy and brush cytological examination of the urethra. of these men 305 (22.23%) had intraepithelial human papillomavirus (confirmed cytolo ... | 1994 | 7966664 |
| human papillomavirus infections in children. | human papillomavirus infections in children, particularly when occurring as condylomata acuminata, present a difficult and often puzzling problem. the possibility that the lesions were acquired through sexual contact mandates a careful and thorough evaluation. even then, the source of the infection may be elusive because of a long latency between inoculation and the development of lesions, the secretive milieu of childhood sexual abuse, and lack of data about modes of transmission. new molecular ... | 1994 | 7970899 |
| transitional cell carcinoma of the bladder: evaluation of the role of human papillomaviruses. | the study evaluated the conflicting results of the role of human papillomavirus (hpv) in the development of bladder carcinoma. | 1994 | 7974942 |
| multinucleated atypia of the vulva. report of a distinct entity not associated with human papillomavirus. | the vulvar mucosa often demonstrates epithelial nuclear atypia in association with reactive and inflammatory conditions. these nuclear changes are usually mild and can be readily distinguished from vulvar intraepithelial neoplasia (vin) and human papillomavirus (hpv)-related lesions. in a recent survey of vulvar biopsies in reproductive-aged women, we identified 12 cases of an unusual pattern of atypia associated with multinucleated epithelial cells but lacking the usual stigmata of reactive cha ... | 1994 | 7977946 |
| analysis of short novel human papillomavirus sequences. | consensus primer mediated pcr protocols have the potential to amplify previously uncharacterized human papillomavirus (hpv) genotypes. in a study on 500 cervicovaginal samples, we amplified four sequences (l1ae 1 to l1ae 4) that failed to hybridize to any of the available hpv type-specific oligonucleotide probes. nucleotide sequencing revealed that the sequences were derived from the l1 region of hitherto unsequenced genotypes. comparison of phylogenetic trees based on the amplified l1 sequences ... | 1994 | 7980548 |
| evaluation of biohit hpv screening and typing kits in detection of human papillomavirus dna from lesions of anogenital tract. | the biohit hpv screening and typing kits for in situ hybridization of human papillomavirus (hpv) dna are now commercially available. the hpv screening kit contains a cocktail of hpv probes, and the typing kit contains separated hybridization probes for hpv 6, 11, 16, 18, 31, and 33. they were evaluated by comparison with an in situ hybridization (ish) method, using the pathogene hpv probes 6/11, 16/18, 31/33/51. one hundred anogenital biopsies from 78 women and 22 men were tested. among them, 43 ... | 1994 | 7981895 |
| oncogene activation: an informative marker for the human papillomavirus-lesions severity. | the aim of this study was the detection of c-ha-ras and c-myc activation and p21ras and p62myc expression in anogenital hpv-associated lesions. thirty-two women with cervical and vaginal lesions and forty men with anal or penile warts were enrolled for the study. biopsies were subjected to histological assessment, in situ hybridization with biotinylated probes, and immunohistochemistry. in both women and men, most of the biopsies showing no major histological abnormalities were negative for hpv ... | 1994 | 7982237 |
| perianal condylomata acuminata. | perianal condylomata, a result of clinical infection with human papillomavirus, are an increasing problem. the warts lead to bleeding, itching, and discomfort in the anal region and also may be associated with anal canal neoplasia. treatment options are numerous and include chemical caustic agents, surgical ablative methods, and immunotherapy. a high rate of recurrence is encountered despite the best of efforts. | 1994 | 7985068 |
| human papilloma virus associated with solitary squamous papilloma complicated by bronchiectasis and bronchial stenosis. | a 28-year-old man presented with recurrent pneumonias for 6 years. chest radiograph and computed tomography showed localized bronchiectasis of the anterior segment of the left upper lobe. bronchoscopy showed bronchial stenosis without an endobronchial lesion. after 6 weeks of antibiotic treatment, the patient had a recurrent pneumonia and underwent left upper lobectomy that showed a solitary squamous papilloma. in situ hybridization studies of the papilloma were reactive for human papilloma viru ... | 1994 | 7988218 |
| [diagnosis of human papillomavirus infections in cervical cytology in the absence of classical signs]. | "nonclassic" cytologic signs (ncs) of infection by human papillomavirus (hpv) were analyzed in two groups of patients: a: (condylomata) 81 women with both condylomata confirmed by biopsy and a cervical smear not showing the "classic" signs of hpv infection, and b: (controls) 50 cervical smears diagnosed as negative. in group a, 96% showed nuclear hyperchromatism, 78% showed perinuclear halos, 77% showed clear cytoplasm, and 74% showed mild koilocytosis. in 89% of the condylomata cases at least f ... | 1993 | 7991848 |
| adjuvant interferon for anal condyloma. a prospective, randomized trial. | the aim of this study was to determine whether interferon combined with surgical excision and fulguration could reduce the unacceptably high rate of recurrence of anal condyloma seen after surgical extirpation. | 1994 | 7995154 |
| presence of antibodies to seven human papillomavirus type 16-derived peptides in cervical cancer patients and healthy controls. | sera from 133 cervical cancer patients and 154 healthy women (controls) from spain and colombia were tested in igg-specific elisas for the presence of antibodies against seven peptides derived from five open-reading frames of human papillomavirus (hpv) type 16. three of the peptides corresponded to overlapping regions of the n-terminal half of e7 protein; the other peptides corresponded to selected regions of e2, e4, l1, and l2 proteins. the prevalence of antibodies against e2 and e7 peptides wa ... | 1994 | 7995981 |
| the association of human papillomavirus with schneiderian papillomas: a dna in situ hybridization study. | human papillomavirus has been identified with dna hybridization techniques in schneiderian papillomas, though results reported in the literature have been conflicting. this study investigated 33 cases of schneiderian papilloma (26 inverted papillomas, seven fungiform papillomas). in situ hybridization using a cocktail of human papillomavirus (hpv) probes 6/11, 16/18, 31/33/35 failed to identify hpv genome in any of the inverted papillomas. this is contrasted by the detection of hpv 6/11 in six o ... | 1994 | 7996631 |
| human papillomavirus vulvitis: a new disease or an unfortunate mistake? | to determine whether human papillomavirus (hpv) was responsible for symptoms in women with vulvar pruritus, pain and superficial dyspareunia who had been referred with a diagnosis of hpv vulvar disease made on clinical and/or colposcopic and/or histological grounds. | 1994 | 7999731 |
| expression and localization of human papillomavirus type 16 e6 and e7 open reading frame proteins in human epidermal keratinocyte. | over 60 different types of human papillomavirus (hpv) have been identified, and they are classified into high and low risk groups based on the risk for malignant progression of hpv associated lesions. hpvs belonging to a high risk group have been shown to express two major transforming proteins, e6 and e7. with respect to the transforming activity of these proteins, many investigators have reported the location of these proteins in the cell, but their results are still controversial. in the pres ... | 1994 | 8009891 |
| coevolution of papillomaviruses with human populations. | the human papillomavirus types that are causally linked to genital cancer originated in ancient times in prehuman primates. the molecular diversity of viral isolates reflects the african origins and the subsequent worldwide spread of human races. the lack of transmission of papillomaviruses between species may support a gradual mode of molecular evolution. | 1994 | 8012758 |
| childhood condyloma accuminatum: association with genital and cutaneous human papillomavirus. | | 1994 | 8018171 |
| human papillomavirus in laryngeal and hypopharyngeal carcinomas. relationship to survival. | to determine the relationship between human papillomavirus (hpv) dna detection in upper aerodigestive tract malignancies and patient outcome. | 1994 | 8018327 |
| cancer associated human papillomaviruses: perinatal transmission and persistence. | to demonstrate the perinatal transmission and persistence of the cancer associated human papillomavirus types 16, 18, 31 and 33. | 1994 | 8018641 |
| human papillomavirus (hpv) type 18 e7 protein is a short-lived steroid-inducible phosphoprotein in hpv-transformed cell lines. | we used a capture elisa to quantify the e7 protein of human papillomavirus type 18 (hpv-18). in hela cells, which express low levels of immunoreactive e7 protein (ie7), ie7 had a mean half-life of 13.5 min. in hpv-18 e7 recombinant baculovirus (e7rec bv)-infected sf21 cells, which express higher levels of e7, the half-life of ie7 was much longer (90 min and > 24 h, with two different e7rec bvs). for two transformed human cervical cell lines expressing hpv-18 e7, exposure of the cells to hydrocor ... | 1994 | 8021595 |
| involvement of human papillomavirus type 20 in epidermodysplasia verruciformis skin carcinogenesis. | the involvement of human papillomavirus (hpv) in skin carcinogenesis in a patient with epidermodysplasia verruciformis was studied. this patient had disseminated pityriasis versicolor-like lesions, flat warts, and a malignant skin carcinoma. hpv types 3, 17, 20 (hpv-20), and 38 were isolated and molecularly cloned from the benign skin lesions of this patient. of these hpvs, only hpv-20 was detected in the malignant skin carcinoma. transcripts of hpv-20 were also expressed in the carcinoma. these ... | 1994 | 8027315 |
| nucleotide sequence and phylogenetic classification of human papillomavirus type 59. | the complete nucleotide sequence of the hpv 59 dna genome, isolated from a vulvar intraepithelial neoplasia, was determined. it consists of 7896 nucleotides. a comparative analysis of this sequence with the sequences of other hpv types revealed the closest homology to hpv 18 (71%), hpv 45 (70%), and hpv 39 (69%). phylogenetic analysis of the complete l1 orfs of hpv 59 and other papillomaviruses exclusively groups all hpvs which have been detected in mucosal lesions into one major branch. this ma ... | 1994 | 8030272 |
| the human papillomavirus type 16 e5 gene cooperates with the e7 gene to stimulate proliferation of primary cells and increases viral gene expression. | the e5 gene from hpv-16 has recently been shown to stimulate anchorage-independent growth of murine 3t3 cells and this phenotype was enhanced in the presence of epidermal growth factor (egf). since egf is capable of stimulating cellular signal transduction, we have compared levels of egf-induced c-fos and c-jun mrna in e5-expressing 3t3 cells. we present data showing that the expression of c-fos and c-jun was higher in e5-expressing 3t3 cells than in control cells. complexes of c-fos/c-jun const ... | 1994 | 8030286 |
| the association of human papillomavirus infection with balanoposthitis: a description of five cases with proposals for treatment. | the features and clinical course of chronic balanitis in 5 patients are presented. in each case, histological examination of a cutaneous biopsy sample showed pronounced features of human papillomavirus (hpv) infection. in addition, hpv dna was demonstrated in each biopsy specimen by a polymerase chain reaction and was found to be type 6 by southern blot hybridization in 4 of the cases. although the association of histological features of hpv infection with balanitis does not prove that hpv is ca ... | 1994 | 8031918 |
| [detection of human papilloma virus dna in tissue sections of cervical carcinoma by in situ hybridization with digoxigenin labelled dna probes]. | formalin fixed, paraffin embedded tissues from 48 cases of squamous carcinoma of the cervix were examined for the presence of human papilloma virus (hpv) dna by in situ hybridization using digoxigenin labelled hpv 6, 11, 16 and 18 dna probes respectively. hpv dna was found in 23 of the tumors (47.92%). hpv 16 dna in 21 tumors (43.75%), hpv 18 dna in four tumors (8.33%), no tumors hybridized to either hpv 6 dna or hpv 11 dna. hpv dna was mainly observed in the nuclei of koilocytic cells and carci ... | 1993 | 8033277 |
| complete reconstitution of conjugation and subsequent degradation of the tumor suppressor protein p53 by purified components of the ubiquitin proteolytic system. | the wild-type tumor suppressor protein p53 is a short-lived protein that plays important roles in regulation of cell cycle, differentiation, and survival. mutations that inactivate or alter the tumor suppressor activity of the protein seem to be the most common genetic change in human cancer and are frequently associated with changes in its stability. the ubiquitin system has been implicated in the degradation of p53 both in vivo and in vitro. a mutant cell line that harbors a thermolabile ubiqu ... | 1994 | 8034027 |
| the inter-relationship of herpes virus, papilloma 16/18 virus infection and pap smear pathology in israeli women. | a previous survey of the seroprevalence of the herpes virus type 2 (hsv-2) infection in israel provided us with an opportunity to study a) the prevalence of human papillomavirus (hpv) 16/18 in selected groups of women; b) the correlation between the infection with hsv-2, hpv-16/18 and papanicolaou (pap) pathology; and c) to identify groups of women who might benefit from routine pap screening. four different population groups of women aged 17-60 years were studied: groups 1 and 2 comprised healt ... | 1994 | 8034488 |
| persistence of type-specific human papillomavirus infection among cytologically normal women. | | 1994 | 8035046 |
| repeatability of interview-derived information on sexual history: a study in women. | we assessed the repeatability of interview-derived information on age at first sexual intercourse and number of sexual partners in women participating in an ongoing prospective study of genital human papillomavirus infection in toronto, canada. of the 100 study participants invited to attend for re-interview twice during their first year in the study, 74 attended for the first follow-up interview about 5 months after recruitment, and 28 of these 74 subjects attended for a second interview about ... | 1994 | 8038255 |
| the value of primary colposcopy in genitourinary medicine--a six year review. | to evaluate the effectiveness of primary colposcopy in the genitourinary medicine setting by comparing the cervical cytology and punch biopsy results for women identified as having an abnormal cervical transformation zone. | 1994 | 8039784 |
| development of human papillomavirus-associated buschke-löwenstein penile carcinoma during cyclosporine therapy for generalized pustular psoriasis. | | 1994 | 8040428 |
| toward objective quality assurance in cervical cytopathology. correlation of cytopathologic diagnoses with detection of high-risk human papillomavirus types. | using the bethesda system, five pathologists independently diagnosed 200 smears that originally had been classified as "atypical," and the results were correlated with concurrent detection of human papillomavirus (hpv) dna by southern analysis and by polymerase chain reaction amplification. the smears were reclassified as benign reactive changes (negative), atypical squamous cells of undetermined significance, or squamous intraepithelial lesion (sil). exact five-way cytologic agreement was achie ... | 1994 | 8042586 |
| allelotype analysis of cervical carcinoma. | to identify the genetic events which may play a role in the development of cervical carcinoma, we performed a detailed allelotype analysis utilizing dna from 53 primary tumors and corresponding normal cells and 57 polymorphic probes mapped to each of the chromosomal arms, excluding the short arms of the acrocentric chromosomes. loss of heterozygosity (loh) of > 25% was observed at sites on 11 chromosomal arms, which included 1q (26%), 3p (35%), 3q (31%), 4q (46%), 5p (53%), 5q (38%), 6p (28%), 1 ... | 1994 | 8044799 |
| hpv-16 e6 and e7 genes, like sv40 early region genes, are insufficient for immortalization of human mesothelial and bronchial epithelial cells. | simian virus 40 (sv40)-transformed human cells usually have a period of increased proliferative potential that terminates in culture crisis, after which immortalization may or may not occur. this study addresses the question of whether human papillomavirus (hpv)-transformed cells may also undergo crisis. two types of human epithelial cells were transfected with either hpv-16 e6 and e7 genes or sv40 early region genes, and all transfectants underwent at least 20 more population doublings than the ... | 1994 | 8050498 |
| lack of synergistic association between human papillomavirus and ras gene point mutation in laryngeal carcinomas. | the spectrum of human papillomavirus (hpv) subtypes in laryngeal carcinomas was investigated by combined polymerase chain reaction (pcr) and restriction fragment length polymorphism analysis. hpv dna was detected in 11 (24%) of 45 cases, including hpv 16 in 9 cases and hpv 18 in 2 cases. other hpv subtypes commonly found in the female genital organs were not detected. in addition, the point mutation of codons 12 and 13 in c-ki-ras-2, c-ha-ras-1, and n-ras genes was studied by the pcr-single-stra ... | 1994 | 8052064 |
| functions of human papillomavirus e6 and e7 oncoproteins. | the identification of certain human papillomaviruses as human tumour viruses has been paralleled by our understanding of how the viral oncoproteins function. the virally encoded e6 and e7 proteins act, in part, to abrogate the activities of the tumour suppressor proteins p53 and prb. the interaction between these viral proteins and regulators of cell growth offers targets for future therapeutic developments. | 1994 | 8055181 |
| [typification of a subgroup of cervical papilloma by in situ hybridization. preliminary study]. | the purpose of this study was to analyze 19 patients infected with human papilloma virus (hpv), in order to determine the most frequent subtype. subjects were patients of the national institute of perinatology (inper), between 1991-1992. all the patients had positive cytology, cervical biopsy was done through colposcopy control. sample characteristics were: mean age 29 years, married; most of them multiparous (9/19) and 7 were monogamous. six out of 12 cytologies were positive for hpv, 12 out of ... | 1994 | 8056363 |
| the ability of human papillomavirus e6 proteins to target p53 for degradation in vivo correlates with their ability to abrogate actinomycin d-induced growth arrest. | functional p53 protein is associated with the ability of cells to arrest in g1 after dna damage. the e6 protein of cancer-associated human papillomavirus type 16 (hpv-16) binds to p53 and targets its degradation through the ubiquitin pathway. to determine whether the ability of e6 to interact with p53 leads to a disruption of cell cycle control, mutated e6 proteins were tested for p53 binding and p53 degradation targeting in vitro, the ability to reduce intracellular p53 levels in vivo, and the ... | 1994 | 8057451 |
| functional studies of e7 proteins from different hpv types. | we have performed comparative studies on the e7 proteins from malignant and non-malignant human papillomavirus types hpv 1, 6, 11, 16, 18, 33). gst/e7 fusion proteins from all these hpv types associate with rb1, p107 and the cyclin a/cdk2 complex. as has been shown for rb1, the association with p107 and cyclin a was weaker for the 'low risk' hpv6 and 11 e7 proteins as compared to 'high risk' hpv16, 18 and 33 e7 proteins. in contrast the e7 protein of the benign type hpv1 bound rb1, p107 and cycl ... | 1994 | 8058327 |
| interaction of p53 with mdm2 is independent of e6 and does not mediate wild type transformation suppressor function. | using a new series of p53 mutants targeting the conserved regions we have analysed the relationship of various activities of the protein. mdm-2 and human papillomavirus (hpv) e6, two proteins which interact with and abrogate p53 function, were shown to bind independently. deletion of the conserved regions of the protein in which most of the naturally occurring mutations are found (boxes ii-v) abrogated transcriptional activity and the ability to interact with e6, supporting the importance of thi ... | 1994 | 8058335 |
| patients as subjects for research: ethical dilemmas for the primary care clinician-investigator. | past studies suggested an association between human papillomavirus (hpv) and cervical intraepithelial neoplasia (cin). in 1987, university of north carolina (unc) hospitals family practice center clinicians were approached for a control population to study this association. | 1994 | 8059623 |
| proliferative verrucous leukoplakia and verrucous hyperplasia. | proliferative verrucous leukoplakia and hyperplasia are irreversible clinicopathologic lesions of the oral cavity and upper respiratory tract with considerable potential for evolving into verrucous or other forms of squamous cell carcinoma. proliferative verrucous leukoplakia is a disease of the oral cavity in which verrucous hyperplasia is a part of its developmental spectrum. verrucous hyperplasia, at other head and neck mucosal sites, may be associated with papillomas or be a de novo lesion. ... | 1994 | 8060064 |
| human papillomavirus-related serological markers of invasive cervical carcinoma in brazil. | masked sera from 194 cases and 217 controls participating in a case-control study of cervical cancer in brazil were examined for antibodies to human papillomavirus (hpv) 16 e6 and e7 by radioimmunoprecipitation assay. radiolabeled full-length e6 and e7 proteins expressed by in vitro transcription and translation in rabbit reticulocyte lysate were used as antigens. the antibody prevalences in cases and controls were: 54.1% versus 6% for e6; 30.4% versus 4.6% for e7; 63.4% versus 10.1% for either ... | 1994 | 8061584 |
| presence of human papillomavirus dna in amniotic fluids of pregnant women with cervical lesions. | amniotic fluids from 37 pregnant women with cervical lesions indicative of human papillomavirus (hpv) infection, detected by histology and slot hybridization, were collected at different times during pregnancy and assayed for hpv using the polymerase chain reaction. hpv dna amplification was obtained in 24 of 37 samples using general primers. of the 24 positive cases, 13, 5, and 0 were of hpv types 16, 18, and 33, respectively, as determined by using specific anticontamination primers. a correla ... | 1994 | 8063239 |
| retinoic acid suppresses human papillomavirus type 16 (hpv16)-mediated transformation of human keratinocytes and inhibits the expression of the hpv16 oncogenes. | we have used a model system of normal hkc and hkc immortalized by transfection with hpv16 dna (hkc/hpv16) to investigate the effect of ra on the growth of hkc/hpv16 and the expression of the hpv16 oncogenes e6 and e7. these studies found that hkc/hpv16 are about 100-fold more sensitive than normal hkc to growth inhibition by ra in both clonal and mass culture growth assays. the precursor to ra, retinol, was also found to be a more potent inhibitor of growth of hkc/hpv16 than normal hkc while bet ... | 1994 | 8067286 |
| genital human papillomavirus infections in young women with vulvar and vestibular papillomatosis. | possible involvement of human papillomaviruses (hpv) in the development of vulvar and vestibular papillomatosis was investigated by using pcr to determine whether hpv dna was present in lesions. fourteen of 272 (5.1%) young women studied were found on gross and histological examination to have vulvar or vestibular papillomatosis. hpv dna sequences were detected in cervicovaginal lavage specimens of 2 of 14 (14.3%) papillomatosis patients and 1 of 17 (5.9%) matched individuals in the control grou ... | 1994 | 8070461 |
| diet and genital warts: a case-control study. | clinical observations support a substantial role for impaired immunity in the development of human papillomavirus (hpv) infections. intake of vitamins, especially vitamins a and c, and alcohol consumption have been reported to influence immune response. | 1994 | 8073343 |
| lethal asphyxiating juvenile laryngeal papillomatosis. a case report with human papillomavirus in situ hybridization analysis. | chronic, progressive, and recurrent laryngeal papillomatosis is an uncommon problem that primarily occurs in children and has been shown (in the pediatric age group) to be caused by human papillomavirus (hpv) types 6 and/or 11. juvenile laryngeal papillomatosis is hallmarked by recurrences, necessitating repeated surgical procedures to maintain airway patency, and is rarely encountered cause of sudden and unexpected death. this case describes a 22-month-old girl who had been treated for laryngea ... | 1994 | 8074107 |
| detection of high-risk human papillomavirus in the cervix and semen of sex partners. | to investigate the possibility of sexual transmission of human papillomavirus (hpv), 53 married couples were examined for the presence of hpv-16 and -18 dnas in the uterine cervix and semen using the polymerase chain reaction method. twenty-three of the 53 women and 12 of the 53 male partners were positive for hpv-16 dna. no hpv-18 dna was detected in samples from any of the partners. in 27 pairs, both partners were negative for hpv dna in cervix or semen; in the remaining 26 pairs, at least 1 o ... | 1994 | 8077728 |
| serological differentiation of human papillomavirus types 11, 16 and 18 using recombinant virus-like particles. | the l1 major capsid protein-coding sequences of human papillomavirus (hpv) types 11, 16 and 18 were expressed in the baculovirus system. virus-like particles (vlps) were purified from recombinant-infected spodoptera frugiperda sf9 cells and cell-free culture supernatants. rabbits immunized with purified vlps developed antibodies that reacted only with the specific vlp type used as the immunogen. in addition, rabbit antibodies raised against infectious hpv-11 virions only reacted with hpv-11 l1 v ... | 1994 | 8077946 |
| the cellular dna polymerase alpha-primase is required for papillomavirus dna replication and associates with the viral e1 helicase. | persistent infection by papillomaviruses involves the maintenance of viral dna as a nuclear plasmid, the replication of which requires host dna polymerases. the role of the cellular dna polymerase alpha-primase holoenzyme was probed by using soluble extracts from rodent cells that replicate bovine papilloma virus 1 and human papilloma virus 6b dna in the presence of the viral e1 helicase and the e2 transcription factor. monoclonal antibodies directed against the catalytic 180-kda subunit of poly ... | 1994 | 8078945 |
| bacterial vaginosis and cervical intraepithelial neoplasia. | in an attempt to investigate an association between the finding of clue cells in papanicolaou-stained (pap) smears and cervical intraepithelial neoplasia (cin), a total of 6150 smears from 1976 were re-investigated. | 1994 | 8079612 |
| penile intraepithelial neoplasia. specific clinical features correlate with histologic and virologic findings. | to evaluate the existence of the morphologic features specific for penile intraepithelial neoplasia (pin), 1000 male sexual partners of women with genital condyloma or intraepithelial neoplasia were studied. | 1994 | 8082079 |
| human papillomavirus in pterygium. | between august 1991 and 1992 consecutive pterygium cases and eight normal conjunctival tissues were examined for the presence of polyclonal human papilloma virus (hpv) antigen by the avidin-biotin-complex technique and 16 of the pterygium cases and 25 pc of the control conjunctivas revealed hpv positivity. in six of the hpv positive pterygium tissues, there were also an epithelial proliferation. in conclusion, hpv infection was not endemic, but sporadic in cukurova region, and it may be contribu ... | 1994 | 8082148 |
| subclinical human papillomavirus infection in a high-risk population. | human papillomavirus (hpv) infection, which has been linked to cervical dysplasia and carcinoma, causes one of the most frequently diagnosed sexually transmitted diseases (stds) in the united states. to determine the prevalence of subclinical hpv infection, to detect any association with dysplasia, and to investigate risk factors, the author studied 249 sexually active adolescent girls and young women in a high-risk population by questionnaire, pelvic examination, cytology, and dot blot nucleic ... | 1994 | 8083064 |
| prevalence of hpv dna in cervical specimens in women with renal transplants: a comparison with dialysis-dependent patients and patients with renal impairment. | the aim of this study was to assess the prevalence of human papillomavirus (hpv) dna in women who had received a renal transplant and to compare this with two control groups. women who had a functioning renal transplant for greater than 6 months (n = 69) were compared with women on maintenance dialysis (n = 89) and women with impaired renal function (creatinine 0.15-0.39 mmol/l) (n = 22). women were excluded if they had had a hysterectomy, were older than 65 years, or were not yet sexually activ ... | 1994 | 8084456 |
| [condyloma acuminatum in female urethra: a case report]. | a case of female intraurethral condyloma acuminata is present in this report. a 52-year-old woman visited our hospital with the chief complaint of miction pain and bloody secretions. thirty seven years previously, she had had a pudental tumor resected which was diagnosed as condyloma acuminata. physical examination revealed two child-finger-head sized, polypoid tumors which arose from about 5 mm inside the external urethral orifice. laboratory findings were negative for rapid plasma reagin card ... | 1994 | 8085526 |
| expression of transforming growth factor-beta 1 and -beta 2 in human papillomavirus (hpv)-associated lesions of the uterine cervix. | human papillomaviruses (hpv) are implicated in the multistep process of cervical carcinogenesis. transforming growth factor beta(tgf-beta) inhibits the proliferation of epithelial cells, and it has also been found to inhibit hpv gene expression in nontumorigenic epithelial cell lines. in the present study, we examined the expression of tgf-beta 1 and tgf-beta 2 protein immunohistochemically (ihc) in a series of 95 hpv-positive and hpv-negative lesions of the uterine cervix, with special emphasis ... | 1994 | 8088612 |
| human papillomavirus detection by hybrid capture and its possible clinical use. | to determine the sensitivity of the hybrid capture method for human papillomavirus (hpv) detection and potential clinical uses as a screening method for the identification of cervical intraepithelial neoplasia. | 1994 | 8089224 |
| [application of antisense oligonucleotides into treatment of human cervical carcinoma]. | antisense oligodeoxynucleotides (oligomers) have critical roles for drugs targeted specifically against oncogenes. we obtained evidence for the inhibitory effects of oligomers to the e6/e7 region of human papilloma virus (hpv) types 16 and 18 on cervical epithelial cells. these cells contained phk16 cells that was the human keratinocytes immortalized by hpv16 full genome transfection, and c4ii cells that was the cervical carcinoma cells harboring hpv 18 genome. the oligomers greatly reduced both ... | 1994 | 8089611 |
| the p53 tumor suppressor protein: meeting review. | | 1993 | 8096197 |
| human papillomavirus and cervical carcinoma in china and taiwan. | | 1993 | 8105200 |
| borderline nuclear abnormality in cervical smears: a cytological review of 200 cases with histological correlation. | the cervical cytology and histology specimens from 200 patients referred to colposcopy with borderline nuclear abnormality were reviewed. human papillomavirus (hpv)-associated changes were identified in 103 of 200 (53%) referral smears and in 139 of 150 (91%) biopsy specimens. cervical intraepithelial neoplasia (cin) was less frequently diagnosed on review compared with the original histopathology reports (30.7% vs 45.4%); the discrepancy was largely attributable to a lower incidence of cini. th ... | 1993 | 8110973 |
| parathyroid hormone-related protein expression in gynecic squamous carcinoma cells. | the regulation of parathyroid hormone-related protein (pth-rp) mrna levels and immunoreactive (ir)pth-rp formation by peptide growth factors, particularly transforming growth factor-beta (tgf-beta) and epidermal growth factor (egf), in squamous cell carcinomas of gynecologic origin is largely unknown. | 1994 | 8111720 |
| multiple plantar epidermoid cysts harboring carcinoembryonic antigen and human papillomavirus dna sequences. | | 1994 | 8113467 |
| functional oligomerization of purified human papillomavirus types 16 and 6b e7 proteins expressed in escherichia coli. | purified non-fused soluble human papillomavirus type 16 and 6b e7 proteins expressed in escherichia coli were found to form oligomers. for both proteins, several degrees of oligomerization were demonstrated by gel filtration, dynamic laser light scattering and scanning electron microscopy. oligomerization was dependent on the concentration of e7 protein. oligomerized e7 proteins were able to bind the retinoblastoma gene product prb and stimulated dna synthesis when introduced into cells. | 1994 | 8113748 |
| [dna identification of human papillomavirus in anogenital condyloma of a male population]. | human papillomaviruses (hpv) are the cause of benign human anogenital lesions where hpv 6 and hpv 11 are most commonly found. conversely, hpv 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. in order to analyze the prevalence of specific hpv types in an argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. these lesions were localized in different regions of the male ... | 1993 | 8114626 |
| transitional cell carcinoma of fossa navicularis in a man with three other synchronous malignancies. | transitional cell carcinoma arising from the anterior portion of the male urethra is rare, with less than 10 cases reported in the literature. our patient had a high-grade, highly invasive transitional cell carcinoma originating in the fossa navicularis and extending proximally along the corpus spongiosum, the corpora cavernosa, and blood and lymphatic vessels. in addition, the patient had a concurrent low-grade prostatic adenocarcinoma, a large basal cell carcinoma of the nose, and a squamous c ... | 1994 | 8116126 |
| hpv detection and p53 alteration in squamous cell verrucous malignancies of the lower genital tract. | we examined five cases of verrucous carcinoma (vc) and two cases of giant condyloma of buschke-löwenstein (gcbl) associated with invasive squamous cell carcinoma (iscc), by immunocytochemistry and molecular techniques. neither human papillomavirus (hpv) footprints nor p53-altered expression and/or mutation were observed among the cases of vc. by contrast, both cases of gcbl with iscc turned out to be hpv 6 or 11 positive, showed overexpression of p53 and, one of the two, a mutation in the nucleo ... | 1993 | 8118602 |
| cervical koilocytosis and high risk hpv types: the benefit of laser vaporization. | forty-six patients with biopsy-proven cervical koilocytosis, which in 19 cases was associated with cin i, were randomized to treatment by cervical laser vaporization or to follow-up without therapy. the polymerase chain reaction was used to examine the paraffin-embedded cervical biopsies for human papillomavirus (hpv) types 6, 11, 16, 18 and 33 by polymerase chain reaction and molecular hybridization technique. twenty biopsies contained hpv dna, in 17 cases of the putatively oncogenic types hpv ... | 1993 | 8119458 |
| human papilloma virus types in routine cytological screening and at colposcopic examinations. | certain types of human papillomaviruses (hpv) play a crucial role in the development of anogenital cellular dysplasia and cancer. we have searched for a broad spectrum of hpv-types by pcr in cervical cell samples from 230 women aged 20-29 years enrolled at routine gynecological health control and 506 women referred to colposcopy due to suspected cytological changes. thirteen percent of the health control women had hpv dna of identified types. half of the colposcopy patients showed benign histolo ... | 1993 | 8119475 |
| human papillomavirus and endocervical adenocarcinoma. | | 1994 | 8119727 |
| risk of genital human papillomavirus infection in women with human immunodeficiency virus-induced immunosuppression. | this study examined the risk factors for genital human papillomavirus (hpv) infection, including hiv-related immunosuppression as well as demographic and behavioral factors. subjects were 207 women with known hiv serologic status, the majority of whom were intravenous drug users recruited from a methadone treatment program in the bronx, new york. a structured questionnaire interview, cervico-vaginal lavage, and cd4 cell measurements were obtained. hpv genomes in the cervico-vaginal cells were de ... | 1994 | 8119767 |
| human papillomavirus type 6 associated buschke-loewenstein tumor (giant condyloma acuminatum). | the term "buschke-loewenstein tumor" has been used for tumors with exophytic, wart-like excrescences in the anogenital region. this broadly includes giant condyloma acuminatum and verrucous carcinoma. we report a 44-year-old japanese man with a giant condyloma acuminatum buschke-loewenstein tumor on the right femoinguinal area. the lesion was a huge grape-like nodule composed of multiple small nodules, reaching 135 x 65 x 40 mm in size. histologically, it showed a regularly acanthotic papilloma ... | 1993 | 8120240 |
| genital herpes simplex virus and human papillomavirus infection. | | 1994 | 8123227 |
| retention of low copy number human papillomavirus dna in cultured cutaneous and mucosal wart keratinocytes. | cultured wart keratinocytes have previously been described as having a limited proclivity to maintain episomal human papillomavirus (hpv) dna. to investigate the nature of episome loss, and to determine keratinocyte-specific factors involved in it, we have examined a large series of anogenital and oral wart keratinocyte cultures, tracing episomal copy number with culture passage. we report that a higher proportion of oral wart keratinocytes maintain episomal hpv dna to first passage (70% compare ... | 1994 | 8126448 |
| carcinoma in situ of the penis. | bowen's disease, erythroplasia of queyrat and bowenoid papulosis are uncommon disorders of the anogenital skin that may be confused with a variety of other lesions. while all appear histologically as carcinoma in situ and are strongly associated with human papillomavirus infection, only bowen's disease and erythroplasia of queyrat have been demonstrated to lead to the development of invasive squamous cell carcinoma. in contrast, bowenoid papulosis has a completely benign course with no present e ... | 1994 | 8126803 |
| urethral meatal warts in men: results of urethroscopy and biopsy. | human papillomavirus is a common sexually transmitted infection associated with cervical dysplasia. we investigated the extent of urethral human papillomavirus infection by performing urethrocystoscopy on 90 men who presented to a sexually transmitted disease clinic with external meatal warts. a total of 83 men underwent biopsies and in 49 human papillomavirus typing was done. of 90 men with external meatal warts 75 (83.3%) had no other warts, while 15 (16.7%) also had more proximal lesions. onl ... | 1994 | 8126814 |
| detection of human papillomavirus dna and state of p53 gene in japanese penile cancer. | the frequency of integration with human papillomavirus (hpv) and its genotypes in japanese penile cancer was examined with relation to p53 gene mutations using polymerase chain reaction amplification. tissues were obtained from 13 patients (eight from freshly frozen and five from paraffin-embedded samples). hpv dna was detected in seven out of the 13 (54%), and their genotypes were type 16 in four, type 31 in one and type 33 in two cases. neither hpv-detected nor -undetected tissues showed mutat ... | 1994 | 8126914 |
| regulation of early gene expression of human papillomavirus type 16 by inflammatory cytokines. | the host's immune reaction against human papillomavirus (hpv) infection remains poorly understood. inflammatory cytokines undoubtedly play a key role through activating and coordinating the immune response. however, their direct interactions with the hpv genes remain unclear. in the present study, the effects of various inflammatory cytokines on hpv16 gene expression were investigated. in a cat assay, tumor necrosis factor (tnf) alpha and interleukin-1 (il-1) alpha were shown to repress hpv16 ea ... | 1994 | 8128617 |
| presence of human papillomavirus infection of the uterine cervix as determined by different detection methods in a low-risk community-based population. | to evaluate the effectiveness of various screening tests for detecting genital human papillomavirus (hpv) in a community-based population and to determine the prevalence of cervical lesions on colposcopically directed biopsies in patients found to have hpv by any screening test. | 1993 | 8130906 |